BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP025268	1,873,836	(T)_5→6	pseudogene (67/1283 nt)	yeaH →	YeaH/YhbH family protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP025268	1,873,831	1	.	T	95.7%	1785.4 / 62.5	515	pseudogene (62/1283 nt)	yeaH	YeaH/YhbH family protein
Reads supporting (aligned to +/- strand): ref base . (13/9); new base T (308/181); total (321/194)
Fisher's exact test for biased strand distribution p-value = 8.22e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 577 total aligned reads displayed.

CAACGACAGCGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGCATACAGTTGGCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGC‑G‑TTT‑T‑T‑ACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATTAATAAGCGTTCGGTGACTGACGTCGACAGCGGCGAATCCGTATCCATTCCCACGGAAGATATTAGCGAACCGATGTTTCATC  >  NZ_CP025268/1873692‑1873964

     aCAGCGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGCATACAGTTGGCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGC‑G‑ttt‑t‑t                                                                                                                                   >  2:7870787/1‑140 (MQ=255)

     aCAGCGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGCATACAGTTGGCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGC‑G‑ttt‑t‑t                                                                                                                                   >  1:8365296/1‑140 (MQ=255)

      cAGCGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGCATACAGTTGGCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGTCCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGC‑G‑ttt‑t‑tt                                                                                                                                  >  1:6816714/1‑139 (MQ=255)

       aGCGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGCATACAGTTGGCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGC‑G‑ttt‑t‑tta                                                                                                                                 <  1:4860349/140‑3 (MQ=255)

       aGCGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGCATACAGTTGGCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGC‑G‑ttt‑t‑tta                                                                                                                                 <  1:6844408/140‑3 (MQ=255)

       aGCGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGCATACAGTTGGCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGC‑G‑ttt‑t‑tta                                                                                                                                 <  1:3532765/140‑3 (MQ=255)

       aGCGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGCATACAGTTGGCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGC‑G‑ttt‑t‑tta                                                                                                                                 <  1:7999625/140‑3 (MQ=255)

         cGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGCATACAGTTGGCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGC‑G‑ttt‑t‑ttacg                                                                                                                               >  1:2993628/1‑136 (MQ=255)

           aaCCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGCATACAGTTGGCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGAATGGTGAATCGCCAGC‑GTTTT‑T‑T‑ACGcc                                                                                                                             >  1:1467661/1‑140 (MQ=255)

            aCCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGCATACAGTTGGCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGC‑GTTTT‑T‑T‑ACGCCg                                                                                                                            <  1:5854050/140‑1 (MQ=255)

                 gggCCTGAGAAGCGGCAACACAGGCGTAGCATACAGTTGGCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGC‑GTTTT‑T‑T‑ACGCCGTtata                                                                                                                       >  1:6974213/1‑140 (MQ=255)

                 gggCCTGAGAAGCGGCAACACAGGCGTAGCATACAGTTGGCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGC‑GTTTT‑T‑T‑ACGCCGTtata                                                                                                                       >  1:6525452/1‑140 (MQ=255)

                     cTGAGAAGCGGCAACACAGGCGTAGCATACAGTTGGCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGC‑GTTTT‑T‑T‑ACGCCGTTATAAAgc                                                                                                                   <  2:1988268/140‑1 (MQ=255)

                     cTGAGAAGCGGCAACACAGGCGTAGCATACAGTTGGCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGC‑GTTTT‑T‑T‑ACGCCGTTATAAAgc                                                                                                                   <  1:1004138/140‑1 (MQ=255)

                       gagaAGCGGCAACACAGGCGTAGCATACAGTTGGCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGC‑GTTTT‑T‑T‑ACGCCGTTATAAAgcgc                                                                                                                 <  1:4163898/140‑1 (MQ=255)

                        agaAGCGGCAACACAGGCGTAGCATACAGTTGGCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGC‑GTTTT‑T‑T‑ACGCCGTTATAAAGCGCa                                                                                                                <  2:1389640/140‑1 (MQ=255)

                        agaAGCGGCAACACAGGCGTAGCATACAGTTGGCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGC‑GTTTT‑T‑T‑ACGCCGTTATAAAGCGCa                                                                                                                >  1:5211436/1‑140 (MQ=255)

                            gCGGCAACACAGGCGTAGCATACAGTTGGCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGC‑GTTTT‑T‑T‑ACGCCGTTATAAAGCGCAAAtt                                                                                                            >  1:2783155/1‑140 (MQ=255)

                               gCAACACAGGCGTAGCATACAGTTGGCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGC‑GTTTT‑T‑T‑ACGCCGTTATAAAGCGCAAATTaaa                                                                                                         <  1:2209563/140‑1 (MQ=255)

                               gCAACACAGGCGTAGCATACAGTTGGCAAATGTAGTACGGGGGGCATATGACCTGGTTTATGGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGC‑GTTTT‑T‑T‑ACGCCGTTATAAAGCGCAAATTaaa                                                                                                         <  2:8970452/140‑1 (MQ=255)

                                   cacaGGCGTAGCATACAGTTGGCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGC‑GTTTT‑T‑T‑ACGCCGTTATAAAGCGCAAATTAAACAGt                                                                                                     <  2:6398476/140‑1 (MQ=255)

                                    acaGGCGTAGCATACAGTTGGCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGC‑GTTTT‑T‑T‑ACGCCGTTATAAAGCGCAAATTAAACAGTc                                                                                                    <  1:111122/140‑1 (MQ=255)

                                     caGGCGTAGCATACAGTTGGCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGC‑GTTTT‑T‑T‑ACGCCGTTATAAAGCGCAAATTAAACAGTCg                                                                                                   <  2:94240/140‑1 (MQ=255)

                                       ggCGTAGCATACAGTTGGCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGC‑GTTTT‑T‑T‑ACGCCGTTATAAAGCGCAAATTAAACAGTCGAt                                                                                                 >  1:2314050/1‑140 (MQ=255)

                                           tAGCATACAGTTGGCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGC‑GTTTT‑T‑T‑ACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTcc                                                                                             <  1:4597627/140‑1 (MQ=255)

                                           tAGCATACAGTTGGCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGC‑GTTTT‑T‑T‑ACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTcc                                                                                             <  2:3908154/140‑1 (MQ=255)

                                             gCATACAGTTGGCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCTTCTTAACGTCAAAAACAAAAGCATGTTGAATCGCCAGCTT‑TTT‑T‑T‑ACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGa                                                                                           <  2:7246324/140‑1 (MQ=255)

                                             gCATACAGTTGGCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGC‑GTTTT‑T‑T‑ACGCCGTTATAAAGCGCACATTAAACAGTCGATCTCCGa                                                                                           >  1:5896537/1‑140 (MQ=255)

                                             gCATACAGTAGGCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGC‑GTTTT‑T‑T‑ACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGa                                                                                           <  1:7051209/140‑1 (MQ=255)

                                              cATACAGTTGGCAAATGTAGTACGGGGGTCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGC‑GTTTT‑T‑T‑AAGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAg                                                                                          >  2:2075241/1‑140 (MQ=255)

                                               aTACTGTTGTCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGC‑GTTTT‑T‑T‑ACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAgg                                                                                         <  2:539603/140‑1 (MQ=255)

                                               aTACAGTTGGCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGC‑GTTTT‑T‑T‑ACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAgg                                                                                         <  1:6163857/140‑1 (MQ=255)

                                               aTACAGTTGGCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGC‑GTTTT‑T‑T‑ACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAgg                                                                                         <  1:7195448/140‑1 (MQ=255)

                                                tACAGTTGGCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGC‑GTTTT‑T‑T‑ACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGc                                                                                        >  1:4766441/1‑140 (MQ=255)

                                                tACAGTTGGCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGC‑GTTTT‑T‑T‑ACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGc                                                                                        >  1:1942114/1‑140 (MQ=255)

                                                 aCAGTTGGCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGC‑GTTTT‑T‑T‑ACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGcc                                                                                       <  2:3089837/140‑1 (MQ=255)

                                                 aCAGTAGGCAACAGTAGGACGGCGGGCATATGACCTGGTTTATTGACCGGCGTCTGACCGGCAAAAACAAACCCATGGAGAATCGCCAGC‑G‑TTT‑T‑TAACGCCGTTATAACGCGCAAATTAAACAGTCGATCTCCGAGGcc                                                                                       <  2:8196257/140‑1 (MQ=255)

                                                  cAGTTGGCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAATCAAAAGCATGGTGAATCGCCAGC‑GTTTT‑T‑T‑ACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCa                                                                                      >  2:7002742/1‑140 (MQ=255)

                                                  cAGTTGGCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGC‑GTTTT‑T‑T‑ACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCa                                                                                      >  1:3886673/1‑140 (MQ=255)

                                                  cAGTTGGCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGC‑GTTTT‑T‑T‑ACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCa                                                                                      >  1:6289471/1‑140 (MQ=255)

                                                  cAGTTGGCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGC‑GTTTT‑T‑T‑ACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCa                                                                                      >  1:9414615/1‑140 (MQ=255)

                                                  cAGTTGGCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGC‑GTTTT‑T‑T‑ACGCCGGTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCa                                                                                      >  2:5817982/1‑140 (MQ=255)

                                                     ttGGCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGC‑GTTTT‑T‑T‑ACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATta                                                                                   >  2:9341326/1‑140 (MQ=255)

                                                      tGGCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGC‑GTTTT‑T‑T‑ACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATtaa                                                                                  >  1:6655145/1‑140 (MQ=255)

                                                      tGGCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGC‑GTTTT‑T‑T‑ACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATtaa                                                                                  <  1:157418/140‑1 (MQ=255)

                                                       ggCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGC‑GTTTT‑T‑T‑ACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATtaat                                                                                 >  1:4968398/1‑140 (MQ=255)

                                                       ggCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGC‑GTTTT‑T‑T‑ACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATtaat                                                                                 >  2:1531522/1‑140 (MQ=255)

                                                       ggCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGC‑GTTTT‑T‑T‑ACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATtaat                                                                                 >  2:1149064/1‑140 (MQ=255)

                                                       ggCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGC‑GTTTT‑T‑T‑ACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATtaat                                                                                 >  1:8926199/1‑140 (MQ=255)

                                                        gCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGC‑GTTTT‑T‑T‑ACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATtaata                                                                                >  2:450620/1‑140 (MQ=255)

                                                        gCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGC‑GTTTT‑T‑T‑ACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATtaata                                                                                >  2:1656255/1‑140 (MQ=255)

                                                             tGTAGGACGGGGGGCATATGACCTGTTTTATTGACCGGCGTCTGAACGGCAAAAACAATAGCATGGTGTCTCGCCAGC‑G‑TTT‑T‑TAAGGACGTTCTTATGCGCATATTACACAGGCGATCTCCGGGGGCATtagtagtggg                                                                           >  2:6797320/1‑135 (MQ=255)

                                                               tagtaCGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGC‑GTTTT‑T‑T‑ACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATTAATAAGCGTTc                                                                         >  1:9238932/1‑140 (MQ=255)

                                                               tagtaCGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGC‑GTTTT‑T‑T‑ACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATTAATAAGCGTTc                                                                         <  2:6313809/140‑1 (MQ=255)

                                                                  taCGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGC‑GTTTT‑T‑T‑ACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATTAATAAGCGTTCGGt                                                                      >  2:3512244/1‑140 (MQ=255)

                                                                  taCGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGC‑GTTTT‑T‑T‑ACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATTAATAAGCGTTCGGt                                                                      >  2:3409230/1‑140 (MQ=255)

                                                                    cGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGC‑GTTTT‑T‑T‑ACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATTAATAAGCGTTCGGtga                                                                    <  2:7569503/140‑1 (MQ=255)

                                                                      gggggCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGC‑GTTTT‑T‑T‑ACGCCGTTATAAAGCGCAAATTAAACAGTCTATCTCCGAGGCCATTAATAAGCGTTCGGtgact                                                                  <  1:3734308/140‑1 (MQ=255)

                                                                      gggggCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGC‑GTTTT‑T‑T‑ACGCCGTTATAAAGCGCAAATTAAACAGTCGATATCCGAGGCCATTAATAAGCGTTCGGtgact                                                                  >  2:9095552/1‑140 (MQ=255)

                                                                       ggggCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGC‑GTTTT‑T‑T‑ACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATTAATAAGCGTTCGGtgactg                                                                 >  1:3999935/1‑140 (MQ=255)

                                                                       ggggCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGC‑GTTTT‑T‑T‑ACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATTAATAAGCGTTCGGtgactg                                                                 >  1:8800970/1‑140 (MQ=255)

                                                                       ggggCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGC‑GTTTT‑T‑T‑ACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATTAATAAGCGTTCGGtgactg                                                                 >  1:3189649/1‑140 (MQ=255)

                                                                       ggggCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGC‑GTTTT‑T‑T‑ACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATTAATAAGCGTTCGGtgactg                                                                 >  2:525600/1‑140 (MQ=255)

                                                                       ggggCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGC‑GTTTT‑T‑T‑ACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATTAATAAGCGTTCGGtgactg                                                                 >  2:4015594/1‑140 (MQ=255)

                                                                       ggggCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGC‑GTTTT‑T‑T‑ACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCAGTAGTAAGCGGTCGGtgacgg                                                                 >  1:6391096/1‑138 (MQ=255)

                                                                         ggCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGC‑GTTTT‑T‑T‑ACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCAGTAATAAGCGTTCGGtgactgac                                                               >  1:267399/1‑140 (MQ=255)

                                                                          gCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGC‑GTTTT‑T‑T‑ACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATTAATAAGCGTTCGGTGACTGACg                                                              >  2:1003375/1‑140 (MQ=255)

                                                                           cctatGCCCTGGTTTATGGACCGGCGTCTGAACGGCATAAACAAAAGCATGGTTAATCGCCAGC‑G‑TTT‑T‑TGACGCCGTTATCAAGCGCTATTTATACAGTCTATCTCCGAGGCCATTAAGAAGCGTTCGGTGACTGACGt                                                             >  2:3630369/3‑140 (MQ=255)

                                                                              atGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGC‑GTTTT‑T‑T‑ACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATTAATAAGCGTTCGGTGACTGACGTCGa                                                          >  2:2232712/1‑140 (MQ=255)

                                                                                   cTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGC‑GTTTT‑T‑T‑ACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATTAATAAGCGTTCGGTGACTGACGTCGACAgcg                                                     >  2:4781818/1‑140 (MQ=255)

                                                                                     ggTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGC‑GTTTT‑T‑T‑ACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATTAATAAGCGTTCGGTGACTGACGTCGACAgcggc                                                   >  2:7462407/1‑140 (MQ=255)

                                                                                     ggTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGC‑GTTTT‑T‑T‑ACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATTAATAAGCGTTCGGTGACTGACGTCGACAgcggc                                                   >  2:46983/1‑140 (MQ=255)

                                                                                     ggTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGC‑GTTTT‑T‑T‑ACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATTAATAAGCGTTCGGTGACTGACGTCGACAgcggc                                                   >  2:6578139/1‑140 (MQ=255)

                                                                                     ggTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGC‑G‑TTT‑TGT‑ACGCCGTTATAAAGAGCAAATTAAAAAGTCGATCTCCGAGGCCAGTAATAAGCTTTCGGTGACTGACTTCGACAgcggc                                                   >  2:4404416/1‑140 (MQ=255)

                                                                                      gTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGC‑GTTTT‑T‑T‑ACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATTAATAAGCGTTCGGTGACTGACGTCGACAgcggcg                                                  <  2:2841214/140‑1 (MQ=255)

                                                                                      gTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGC‑GTTTT‑T‑T‑ACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATTAATAAGCGTTCGGTGACTGACGTCGACAgcggcg                                                  <  1:334504/140‑1 (MQ=255)

                                                                                      gTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGC‑GTTTT‑T‑T‑ACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATTAATAAGCGTTCGGTGACTGACGTCGACAgcggcg                                                  <  2:8342939/140‑1 (MQ=255)

                                                                                          aTTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGC‑GTTTT‑T‑T‑ACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATTAATAAGCGTTCGGTGACTGACGTCGACAGCGGCGAATc                                              >  1:741904/1‑140 (MQ=255)

                                                                                           ttGACCGGCGTCTGAACGACAAAAACAAAAGCATGGTGAATCGCCAGC‑GTTTT‑T‑T‑ACGCCGTTATAACGCGCAAATTAAACAGTCGATCTCCGAGGCCCTTAATAAGCGTTCGGTGCCTGACGTCGACAGCGGCGAATcc                                             >  2:4882051/1‑140 (MQ=255)

                                                                                            tGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGC‑GTTTT‑T‑T‑ACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATTAATAAGCGTTCGGTGACTGACGTCGACAGCGGCGAATCCg                                            >  2:3774535/1‑140 (MQ=255)

                                                                                                cGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGC‑GTTTT‑T‑T‑ACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATTAATAAGCGTTCGGTGACTGACGTCGACAGCGGCGAATCCGTATc                                        >  1:7785787/1‑140 (MQ=255)

                                                                                                cGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGC‑GTTTT‑T‑T‑ACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATTAATAAGCGTTCGGTGACTGACGTCGACAGCGGCGAATCCGTATc                                        >  1:1855545/1‑140 (MQ=255)

                                                                                                     tCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGC‑GTTTT‑T‑T‑ACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATTAATAAGCGTTCGGTGACTGACGTCGACAGCGGCGAATCCGTATCCATTc                                   >  2:8759754/1‑140 (MQ=255)

                                                                                                     tCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGC‑GTTTT‑T‑T‑ACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATTAATAAGCGTTCGGTGACTGACGTCGACAGCGGCGAATCCGTATCCATTc                                   >  2:6144084/1‑140 (MQ=255)

                                                                                                          aCGGCAAAAACAAAAGCATGGTGAATCGCCAGC‑GTTTT‑T‑T‑ACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATTAATAAGCGTTCGGTGACTGACGTCGACAGCGGCGAATCCGTATCCATTCCCACg                              <  1:757301/140‑1 (MQ=255)

                                                                                                          aCGGCAAAAACAAAAGCATGGTGAATCGCCAGC‑GTTTT‑T‑T‑ACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATTAATAAGCGTTCGGTGACTGACGTCGACAGCGGCGAATCCGTATCCATTCCCACg                              <  2:2783155/140‑1 (MQ=255)

                                                                                                            ggCAAAAACAAAAGCATGGTGAATCGCCAGC‑GTTTT‑T‑T‑ACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATTAATAAGCGTTCGGTGACTGACGTCGACAGCGGCGAATCCGTATCCATTCCCACGGa                            <  1:8160581/140‑1 (MQ=255)

                                                                                                             gCAAAATCAAAAGCATGGAGAATCGCCACC‑G‑TTTAT‑T‑ACGCCGTTATAAAGCGCAACTTAAACAGGCGCACTCCGAGGCCATTAAAACGCGAACGGTGACAGACGTCGACAGCGGCGAATCCGTATCCATTCCCACGGaa                           <  2:4174146/140‑1 (MQ=255)

                                                                                                             gCAAAAACAAAAGCATGGTGAATCGCCAGC‑GTTTT‑T‑T‑ACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATTAATAAGCGTTCGGTGACTGACGTCGACAGCGGCGAATCCGTATCCATTCCCACGGaa                           >  1:9311838/1‑140 (MQ=255)

                                                                                                                      aaaGCATGGTGAATCGCCAGC‑GTTTT‑T‑T‑ACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATTAATAAGCGTTCGGTGACTGACGTCGACAGCGGCGAATCCGTATCCATTCCCACGGAAGATATTAGc                  >  2:3235527/1‑140 (MQ=255)

                                                                                                                           aTGGTGAATCGCCAGC‑GTTTT‑T‑T‑ACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATTAATAAGCGTTCGGTGACTGACGTCGACAGCGGCGAATCCGTATCCATTCCCACGGAAGATATTAGCGAAcc             <  2:7902981/140‑1 (MQ=255)

                                                                                                                            tGGTGAATCGCCAGC‑GTTTT‑T‑T‑ACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATTAATAAGCGTTCGGTGACTGACGTCGACAGCGGCGAATCCGTATCCATTCCCACGGAAGATATTAGCGAACCg            <  1:8619917/140‑1 (MQ=255)

                                                                                                                              gagAATCGCCAGC‑GTTTT‑T‑T‑ACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATTAATAAGCGTTCGGTGACTGACGTCGACAGCGGCGAATCCGTATCCATTCCCACGGAAGATATTAGCGAACCGAt          <  1:9125559/138‑1 (MQ=255)

                                                                                                                              gTGAATCGCCAGC‑GTTTT‑T‑T‑ACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATTAATAAGCGTTCGGTGACTGACGTCGACAGCGGCGAATCCGTATCCATTCCCACGGAAGATATTAGCGAACCGAt          >  1:3649545/1‑140 (MQ=255)

                                                                                                                              ctGAATCGCCAGC‑GTTTT‑T‑T‑ACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATTAATAAGCGTTCGGTGACTGACGTCGACAGCGGCGAATCCGTATCCATTCCCACGGAAGATATTAGCGAACCGAt          <  2:5660701/139‑1 (MQ=255)

                                                                                                                                gAATCGCCAGC‑GTTTT‑T‑T‑ACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATTAATAAGCGTTCGGTGACTGACGTCGACAGCGGCGAATCCGTATCCATTCCCACGGAAGATATTAGCGAACCGATGt        <  1:2544743/140‑1 (MQ=255)

                                                                                                                                 aaTCGCCAGC‑GTTTT‑T‑T‑ACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATTAATAAGCGTTCGGTGACTGACGTCGACAGCGGCGAATCCGTATCCATTCCCACGGAAGATATTAGCGAACCGATGtt       >  2:2474779/1‑140 (MQ=255)

                                                                                                                                   tCGCCAGC‑GTTTT‑T‑T‑ACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATTAATAAGCGTTCGGTGACTGACGTCGACAGCGGCGAATCCGTATCCATTCCCACGGACGATATTAGCGAACCGATGTTtc     >  1:559632/1‑140 (MQ=255)

                                                                                                                                      ccAGC‑GTTTT‑T‑T‑ACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATTAATACGCGTTCGGTGACTGACGTCGACAGCGGCGAATCCGTATCCATTCCCACGGAAGATATTAGCGAACCGATGTTtcatc  <  1:4301467/140‑1 (MQ=255)

                                                                                                                                      ccAGC‑GTTTT‑T‑T‑ACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATTAATAAGCGTTCGGTGACTGACGTCGACAGCGGCGAATCCGTATCCATTCCCACGGAAGATATTAGCGAACCGATGTTtcatc  <  2:2421128/140‑1 (MQ=255)

CAACGACAGCGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGCATACAGTTGGCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGC‑G‑TTT‑T‑T‑ACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATTAATAAGCGTTCGGTGACTGACGTCGACAGCGGCGAATCCGTATCCATTCCCACGGAAGATATTAGCGAACCGATGTTTCATC  >  NZ_CP025268/1873692‑1873964

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`