BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP025268	1,913,492	+T	coding (88/144 nt)	mgrB ←	PhoP/PhoQ regulator MgrB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP025268	1,913,492	1	.	T	99.4%	2013.1 / 1.0	524	coding (88/144 nt)	mgrB	PhoP/PhoQ regulator MgrB
Reads supporting (aligned to +/- strand): ref base . (2/1); new base T (295/226); total (297/227)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 543 total aligned reads displayed.

TTCGTTCATTTCACCACCTCTGAGTAGAGGCGCTATTCTACCACTGCTGGAGAGGAAGAAAATCTAGTGCTGAAAAAATGATATCACCACGGGATAAACTGGTTAATGGCACAAATTCCGCTGAAAAATTGTACATCCTG‑ATCGCACATCATGTTGAATACCTGCGCCCAAAGCAGCAAGCAAGCCAACACCACGACAACCAGAACGACCCATCGAAACTTTTTCACTCCAATCTCCGTTTCACCTACCTTATGTCATTAAACTAGCATGCGT  >  NZ_CP025268/1913353‑1913625

     tCATTTCACCACCTCTGAGTAGAGGCGCTATTCTACCACTGCTGGAGAGGAAGAAAATCTAGTGCTGAAAAAATGATATCACCACGGGATAAACTGGTTAATGGCACAAATTCCGCTGAAAAATTGTACATACTgtatcg                                                                                                                                   <  2:7420074/140‑6 (MQ=255)

      cATTTCACCACCTCTGAGTAGAGGCGCTATTCTACCACTGCTGGAGAGGAAGAAAATCTAGTGCTGAAAAAATGATATCACCACGGGATAAACTGGTTAATGGCACAAATTCCGCTGAAAAATTGTACATCCTGTATCGc                                                                                                                                  <  2:9253465/140‑1 (MQ=255)

         ttCACCACCTCTGAGTAGAGGCGCTATTCTACCACTGCTGGAGAGGAAGAAAATCTAGTGCTGAAAAAATGATATCACCACGGGATAAACTGGTTAATGGCACAAATTCCGCTGAAAAATTGTACATCCTGTATCGcaca                                                                                                                               >  1:3954934/1‑140 (MQ=255)

            accaccTCTGAGTAGAGGCGCTATTCTACCACTGCTGGAGAGGAAGAAAATCTAGTGCTGAAAAAATGATATCACCACGGGATAAACTGGTTAATGGCACAAATTCCGCTGAAAAATTGTACATCCTGTATCGCAcatca                                                                                                                            <  1:4219490/140‑1 (MQ=255)

            accaccTCTGAGTAGAGGCGCTATTCTACCACTGCTGGAGAGGAAGAAAATCTAGTGCTGAAAAAATGATATCACCACGGGATAAACTGGTTAATGGCACAAATTCCGCTGAAAAATTGTACATCCTGTATCGCAcatca                                                                                                                            <  1:4759035/140‑1 (MQ=255)

                      aGTAGAGGCGCTATTCTACCACTGCTGGAGAGGAAGAAAATCTAGTGCTGAAAAAATGATATCACCACGGGATAAACTGGTTAATGGCACAAATTCCGCTGAAAAATTGTACATCCTGTATCGCACATCATGTTGAATAc                                                                                                                  >  2:6091847/1‑140 (MQ=255)

                      aGTAGAGGCGCTATTCTACCACTGCTGGAGAGGAAGAAAATCTAGTGCTGAAAAAATGATATCACCACGGGATAAACTGGTTAATGGCACAAATTCCGCTGAAAAATTGTACATCCTGTATCGCACATCATGTTGAATAc                                                                                                                  >  1:5700248/1‑140 (MQ=255)

                      aGTAGAGGCGCTATTCTACCACTGCTGGAGAGGAAGAAAATCTAGTGCTGAAAAAATGATATCACCACGGGATAAACTGGTTAATGGCACAAATTCCGCTGAAAAAATGTACATCCTGTATCGCACATCATGTTGAATAc                                                                                                                  >  1:2758728/1‑140 (MQ=255)

                           agGCGCTATTCTACCACTGGTGGAGAGGAAGAAAATCTAGTGCTGAAAAAATGATATCACCACGGGATAAACTGGTTAATGGCACAAATTCCGCTGAAAAATTGTACATCCTGTATCGCACATCATGTTGAATACCTgcg                                                                                                             >  1:2970698/1‑140 (MQ=255)

                           agGCGCTATTCTACCACTGCTGGAGAGGAAGAAAATCTAGTGCTGAAAAAATGATATCACCACGGGATAAACTGGTTAATGGCACAAATTCCGCTGAAAAATTGTACATCCTGTATCGCACATCATGTTGAATACCTgcg                                                                                                             >  1:6023757/1‑140 (MQ=255)

                           agGCGCTATTCTACCACTGCTGGAGAGGAAGAAAATCTAGTGCTGAAAAAATGATATCACCACGGGATAAACTGGTTAATGGCACAAATTCCGCTGAAAAATTGTACATCCTGTATCGCACATCATGTTGAATACCTgcg                                                                                                             >  1:3579460/1‑140 (MQ=255)

                           agGCGCTATTCTACCACTGCTGGAGAGGAAGAAAATCTAGTGCTGAAAAAATGATATCACCACGGGATAAACTGGTTAATGGCACAAATTCCGCAGAAAAATTGTACATCCTGTATCGCACATCATGTTGAATACCTgcg                                                                                                             >  2:5972588/1‑140 (MQ=255)

                           agGCGCTATTCTACCACTGCGGGAGAGGAAGAAAATCAAGTGCTGAAAAAATGATATCACCACGGGATAAACTGGTTAATGGCACAAATTCCGCTGAAAAATTGTACATCCTGTCTCGCACATCAGGTTGAATACCTgcg                                                                                                             >  2:2273040/1‑140 (MQ=255)

                           agGCGCTATTCTACCAAAGCTGGAGAGGAAGAAAATCAAGTGCTTAAAAAATTATATCACCACGGGATAAAATGGTTAATGGCACACATTCCGCTGAAAAATTGTACAACCTGTATAGCACATCATGTTGAATACCTgcg                                                                                                             >  1:2798340/1‑140 (MQ=255)

                             gcgcTATTCTACCACTGCTGGAGAGGAAGAAAATCTAGTGCTGAAAAAATGTTATCACCACGGGATTAACTGGTTAATGGCACAAATTCCGCTGAAAAATTGTACATCCTGTATCGCACATCATGTTGAATACCTGCGcc                                                                                                           <  2:445381/140‑1 (MQ=255)

                             gcgcTATTCTACCACTGCTGGAGAGGAAGAAAATCTAGTGCTGAAAAAATGATATCACCACGGGATAAACTGGTTAATGGCACAAATTCCGCTGAAAAATTGTACATCCTGTATCGCACATCATGTTGAATACCTGCGcc                                                                                                           >  2:3978904/1‑140 (MQ=255)

                               gcTATTCTACCACTGCTGGAGAGGAAGAAAATCTAGTGCTGAAAAAATGATATCACCACGGGATAAACTGGTTAATGGCACAAAGTCCGCTGAAAAATTGTACATCCTGTATCGCACATCATGTTGAATACCTGCGCCCa                                                                                                         <  2:3064664/140‑1 (MQ=255)

                                cTATTCTACCACTGCTGTAGAGGAAGAAAATCTAGTGCTGAAAAAATGATATCACCACGGGATAAACTGGTTAATGGCACAAATTCCGCTGAAAAATTGTACATCCTGTATCGCACATCAAGTTGAATACCTGCGCCCaa                                                                                                        >  2:161565/1‑140 (MQ=255)

                                cTATTCTACCACTGCTGGAGAGGACGAAAATCTAGTGCTGAAAAAATGATATCACCACGGGATAAACTGGTTAATGGCACAAATTCCGCGGAAAAATTGTTCATCCTGTATCGCACATCATGTTGAATACCTGCGCCCaa                                                                                                        >  1:9157455/1‑140 (MQ=255)

                                cTATTCTACCACTGCTGGAGAGGAAGAAAATCTAGTGCTGAAAAAATGATATCACCACGGGATAATCTGGTTAATGGCACAAATTCCGCTGAAAAATTGTACATCCTGTATCGCACATCATGTTGAATACCTGCGCCCaa                                                                                                        >  1:7981229/1‑140 (MQ=255)

                                cTATTCTACCACTGCTGGAGAGGAAGAAAATCTAGTGCTGAAAAAATGATATCACCACGGGATAAACTGGTTAATGGCACAAATTCCGCTGAAAAATTGTACATCCTGTATCGCACATCATGTTGAATACCTGCGCCCaa                                                                                                        >  2:2199966/1‑140 (MQ=255)

                                  aTTCTACCACTGCTGGAGAGGAAGAAAATCTAGTGCTGAAAAAATGATATCACCACGGGATAAACTGGTTAATTGCACAAATTCCGCTGAAAAATTGTACATCCAGTATCGCACATCATGTTGAATACCTGCGCCCAAag                                                                                                      >  1:7253987/1‑140 (MQ=255)

                                        ccACTGCTGGAGAGGAAGAAAATCTAGTGCTGAAAAAATGATATCACCACGGGATAAACTGGTTAATGGCACAAATTCCGCTGAAAAATTGTACATCCTGTATCGCACATCATGTTGAATACCTGCGCCCAAAGCagcaa                                                                                                >  1:1864836/1‑140 (MQ=255)

                                         cACTGCTGGAGAGGAAGAAAATCTAGTGCTGAAAAAATGATATCACCACGGGATAAACTGGTTAATGGCACAAATTCCGCTGAAAAATTGTACATCCTGTATCGCACATCATGTTGAATACCTGCGCCCAAAGCagcaag                                                                                               <  2:75144/140‑1 (MQ=255)

                                         cACTGCTGGAGAGGAAGAAAATCTAGTGCTGAAAAAATGATATCACCACGGGATAAACTGGTTAATGGCACAAATTCCGCTGAAAAATTGTACATCCTGTATCGCACATCATGTTGAATACCTGCGCCCAAAGCagcaag                                                                                               <  2:4617529/140‑1 (MQ=255)

                                         cACTGCTGGAGAGGAAGAAAATCTAGTGCTGAAAAAATGATATCACCACGGGATAAACTGGTTAATGGCACAAATTCCGCTGAAAAATTGTACATCCTGTATCGCACATCATGTTGAATACCTGCGCCCAAAGCagcaag                                                                                               <  1:6710841/140‑1 (MQ=255)

                                         cACTGCTGGAGAGGAAGAAAATCTAGTGCTGAAAAAATGATATCACCACGGGATAAACTGGTTAATGGCACAAATTCCGCTGAAAAATTGTACATCCTGTATCGCACATCATGTTGAATACCTGCGCCCAAAGCagcaag                                                                                               <  1:1759300/140‑1 (MQ=255)

                                         cACTGCTGGAGAGGAAGAAAATCTAGTGCTGAAAAAATGATATCACCACGGGATAAACTGGTTAATGGCACAAATTCCGCTGAAAAATTGTACATCCTGTATCGCACATCATGTTGAATACCTGCGCCCAAAGCagcaag                                                                                               <  2:7065543/140‑1 (MQ=255)

                                           ctgctgGAGAGGAAGAAAATCTAGTGCTGAAAAAATGATATCACCACGGGATAAACTGGTTAATGGCACAAATTCCGCTGAAAAATTGTACATCCTGTATCGCACATCATGTTGAATACCTGCGCCCAAAGCagcaagca                                                                                             >  2:2070985/1‑140 (MQ=255)

                                           ctgctgGAGAGGAAGAAAATCTAGTGCTGAAAAAATGATATCACCACGGGATAAACTGGTTAATGGCACAAATTCCGCTGAAAAATTGTACATCCTGTATCGCACATCATGTTGAATACCTGCGCCCAAAGCagcaagca                                                                                             >  1:7599355/1‑140 (MQ=255)

                                             gctgGAGAGGAAGACAATCTAGTGCTGAAAAAATGATATCACCACGGGATAAACTGGTTAATGGCACAAATTCCGCTGAAAAATTGTACATCCTGTATCGCACATCATGTTGAATACCTGCGCCCAAAGCAGcaagcaag                                                                                           >  2:577747/1‑140 (MQ=255)

                                             gctgGAGAGGAAGAAAATCTAGTGCTGAAAAAATGATATCACCACGGGATAAACTGGTTAATGGCACAAATTCCGCTGAAAAATTGTACATCCTGTATCGc                                                                                                                                  <  2:1567143/101‑1 (MQ=255)

                                             gctgGAGAGGAAGAAAATCTAGTGCTGAAAAAATGATATCACCACGGGATAAACTGGTTAATGGCACAAATTCCGCTGAAAAATTGTACATCCTGTATCGCACATCATGTTGAATACCTGCGCCCAAAGCAGcaagcaag                                                                                           >  2:7713303/1‑140 (MQ=255)

                                             gctgGAGAGGAAGAAAATCTAGTGCTGAAAAAATGATATCACCACGGGATAAACTGGTTAATGGCACAAATTCCGCTGAAAAATTGTACATCCTGTATCGCACATCATGTTGAATACCTGCGCCCAAAGCAGcaagcaag                                                                                           >  1:3107826/1‑140 (MQ=255)

                                             gctgGAGAGGAAGAAAATCTAGTGCTGAAAAAATGATATCACCACGGGATAAACTGGTTAATGGCACAAATTCCGCTGAAAAATTGTACATCCTGTATCGCACATCATGTTGAATACCTGCGCCCAAAGCAGcaagcaag                                                                                           >  1:8478795/1‑140 (MQ=255)

                                             gctgGAGAGGAAGAAAATCTAGTGCTGAAAAAATGATATCACCACGGGATAAACTGGTTAATGGCACAAATTCCGCTGAAAAATTGTACATCCTGTATCGCACATCATGTTGAATACCTGCGCCCAAAGCAGcaagcaag                                                                                           >  2:5085545/1‑140 (MQ=255)

                                             gctgGAGAGGAAGAAAATCTAGTGCTGAAAAAATGATATCACCACGGGATAAACTGGTTAATGGCACAAATTCCGCTGAAAAATTGTACATCCTGTATCGCACATCATGTTGAATACCTGCGCCCAAAGCAGcaagcaag                                                                                           >  2:8457138/1‑140 (MQ=255)

                                             gctgGAGAGGAAGAAAATCTAGTGCTGAAAAAATGATATCACCACGGGATAAACTGGTTAATGGCACAAATTCCGCTGAAAAATTGTACATCCTGTATCGCACATCATGTTGAATACCTGCGCCCAAAGCAGcaagcaag                                                                                           >  2:1263935/1‑140 (MQ=255)

                                             gctgGAGAGGAAGAAAATCTAGTGCTGAAAAAATGATATCACCACGGGATAAACTGGTTAATGGCACAAATTCCGCTGAAAAATTGTACATCCTGTATCGCACATCATGTTGAATACCTGCGCCCAAAGCAGcaagcaag                                                                                           >  1:4107419/1‑140 (MQ=255)

                                             gctgGAGAGGAAGAAAATCTAGTGCTGAAAAAATGATATCACCACGGGATAAACTGGTTAATGGCACAAATTCCGCTGAAAAATTGTACATCCTGTATCGCACATCATGGTGAATACCTGCGCCCAAAGCAGcaagcaag                                                                                           >  2:3793422/1‑140 (MQ=255)

                                             gctgGAGAGGAAGAAAATCTAGTGATGAAAAAATGATATCACCACGGGATTATCTGGTTATTGGCACAAATTCCGGTGAAAGGTTGTTCATCCTGTTTGGCTCATCTTGTTGATTACCTGCGCCCAAAGCTGctagcaag                                                                                           >  1:1480256/1‑140 (MQ=255)

                                              ctgGAGAGGAAGAAAATCTAGTGCTGAAAAAATGATATCACCACGGGATAAACTGGTTAATGGCACAAATTCCGCTGAAAAATTGTACATCCTGTATCGCACATCATGTTGAATACCTGCGCCCAAAGCAGCaagcaagc                                                                                          >  2:8020661/1‑140 (MQ=255)

                                               tgGAGAGGAAGAAAATCTAGTGCTGAAAAAATGATATCACCACGGGATAAACTGGTTAATGGCACAAATTCCGCTGAAAAATTGTACATCCTGTATCGCACATCATGTTGAATACCTGCGCCCAAAGCAGCAAGCAAGcc                                                                                         <  1:3325852/140‑1 (MQ=255)

                                                 gagaGGAAGAAAATCTAGTGCTGAAAAAATGATATCACCACGGGATAAACTGGTTAATGGCACAAATTCCGCTGAAAAATTGTACATCCTGTATCGCACATCATGTTGAATACCTGCGCCCAAAGCAGCAAGCAAGCCaa                                                                                       <  2:102189/140‑1 (MQ=255)

                                                  agagGAAGAAAATCTAGTGCTGAAAAAATGATAACACCACGGGATAAACTGGTTAAAGGCACAAATTCCGCTGAAAAATTGTACATCCTGTATCGCACATCATGTTGAATACCTGAGCCCAAAGCAGCAACCAAGCCAac                                                                                      >  2:6398236/1‑140 (MQ=255)

                                                        agatAATCTAGTGCTGAAAAAATGATATCACCACGGGATAAACTGGTTAATGGCACAAATTCCGCTGAAAATTTGTACATCCTGTATCGCACATCATGTTGAATACCTGCGCCCAAAGCAGCAAGCAAGCCAACACCACg                                                                                <  2:561686/140‑1 (MQ=255)

                                                        agaaAATCTAGTGCTGAAAATATGATATCACCACGGGATAAACTGGTTTATGGCACAAATTCCGCTGAAAAATTGTACATCCTGTATCGCACATCATGTTGAATACCTGCGCCCAAAGCAGCAAGCAAGCCAACACCACg                                                                                >  2:2677824/1‑140 (MQ=255)

                                                        agaaAATCTAGTGCTGAAAAAATGATATCACCACGGGATAAACTGGTTAATGGCACAAATTCCGCTGAAAAATTGTACATCCTGTATCGCACATCATGTTGAATACCTGCGCCCAAAGCAGCAAGCAAGCCAACACCACg                                                                                <  2:2323140/140‑1 (MQ=255)

                                                        agaaAATCTAGTGCTGAAAAAATGATATCACCACGGGATAAACTGGTTAATGGCACAAATTCCGCTGAAAAATTGTACATCCTGTATCGCACATCATGTTGAATACCTGCGCCCAAAGCAGCAAGCAAGCCAACACCACg                                                                                <  2:1838803/140‑1 (MQ=255)

                                                        agaaAATCTAGTGCTGAAAAAATGATATCACCACGGGATAAACAGGTTAATGGCACAAATTCCGCTGAAAAATTGTACATCCTGTATCGCACATCATGTTGAATACCTGCGCCCAAAGCAGCAAGCAAGCCAACACCACg                                                                                >  2:5458542/1‑140 (MQ=255)

                                                                 aGTGCTGAAAAAATGATATCACCACGGGATAAACTGGTTAATGGCACAAATTCCGCTGAAAAATTGTACATCCTGTATCGCACATCATGTTGAATACCTGCGCCCAAAGCAGCAAGCAAGCCAACACCACGACAACCAGa                                                                       >  1:3807894/1‑140 (MQ=255)

                                                                  gTGCTGAAAAAATGATATCACCACGGGATAAACTGGTTAATGGCACAAATTCCGCTGAAAAATTGTACATCCTGTATCGCACATCATGTTGAATACCTGCGCCCAAAGCAGCAAGCAAGCCAACACCACGACAACCAGaa                                                                      >  2:8255603/1‑140 (MQ=255)

                                                                   tGCTGAAAAAATGATATCACCACGGGATAAACTGGTTAATGGCACAAATTCCGCTGAAAAATTGTACATCCTGTATCGCACATCATGTTGAATACCTGCGCCCAAAGCAGCAAGCAAGCCAACACCACGACAACCAGAAc                                                                     >  1:5021535/1‑140 (MQ=255)

                                                                     cTGAAAAAATGATATCACCACGGGATAAACTGGTTAATGGCACAAATTCCGCTGAAAAATTGTACATCCTGTATCGCACATCATGTTGAATACCTGCGCCCAAAGCAGCAAGCAAGCCAACACCACGACAACCAGAACGa                                                                   <  1:7086134/140‑1 (MQ=255)

                                                                                    caccacGGGATAAACTGGTTAATGGCACAAATTCCGCTGAAAAATTGTACATCCTGTATCGCACATCATGTTGAATACCTGCGCCCAAAGCAGCAAGCAAGCCAACACCACGACAACCAGAACGACCCATCGAAACtttt                                                    >  1:6785648/1‑140 (MQ=255)

                                                                                       cacGGGATAAACTGGTTAATGGCACAAATTCCGCTGAAAAATTGTACATCCTGTATCGCACATCATGTTGAATACCTGCGCCCAAAGCAGCAAGCACGCCAACACCACGACAACCAGAAAGACCCATCGAAACTTTTTCa                                                 >  1:4390848/1‑140 (MQ=255)

                                                                                         cGGGATAAACTGGTTAATGGCACAAATGNCGCTGAAAAATTGTACATCCTGTATCGCACATCATGTTGAATACCTGCGCCCAAAGCAGCAAGCAAGCCAACACCACGACAACCAGAACGACCCATCGAAACTTTTTCACt                                               <  1:5563141/140‑1 (MQ=255)

                                                                                          gggATAAACTTGTTAATGGCACAAATTCCGCTGAAAAATTGTACATCCTGTATCGCACATCATGTTGAATACCTGCGCCCAAAGCAGCAAGCAAGCCAACACCACGACAACCAGAACGACCCATCGAAACTTTTTCACTc                                              <  2:2518651/140‑1 (MQ=255)

                                                                                            gATAAACTGGTTAATGGCACAAATTCCGCTGAAAAATTGTACATCCTGTATCGCACATCATGTTGAATACCTGCGCCCAAAGCAGCAAGCAAGCCAACACCACGACAACCAGAACGACCCATCGAAACTTTTTCACTCCa                                            >  1:837779/1‑140 (MQ=255)

                                                                                            gATAAACTGGTTAATGGCACAAATTCCGCTGAAAAATTGTACATCCTGTATCGCACATCATGTTGAATACCTGCGCCCAAAGCAGCAAGCAAGCCAACACCACGACAACCAGAACGACCCATCGAAACTTTTTCACTCCa                                            >  1:241559/1‑140 (MQ=255)

                                                                                              tAAACTGGTTAATGGCACAAATTCCGCTGAAAAATTGTACATCCTGTATCGCACATCATGTTGAATACCTGCGCCCCAAGCAGCAAGCAAGCCAACACCACGACAACCAGAACGACCCATCGAAACTTTTTCACTCCAAt                                          <  2:3676047/140‑1 (MQ=255)

                                                                                                aaCTGGTTAATGGCACAAATTCCGCTGAAAAATTGTACATCCTGTATCGCACATCATGTTGAATACCTGCGCCCAAAGCAGCAAGCAAGCCAACACCACGACAACCAGAACGACCCATCGAAACTTTTTCACTCCAAtct                                        >  2:8601226/1‑140 (MQ=255)

                                                                                                aaCTGGTTAATGGCACAAATTCCGCTGAAAAATTGTACATCCTGTATCGCACATCATGTTGAATACCTGCGCCCAAAGCAGCAAGCAAGCCAACACCACGACAACCAGAACGACCCATCGAAACTTTTTCACTCCAAtct                                        >  2:2931885/1‑140 (MQ=255)

                                                                                                 aCTGGTTAATGGCACAAATTCCGCTGAAAAATTGTACATCCTGTATCGCACATCATGTTGAATACCTGCGCCCAAAGCAGCAAGCAAGCCAACACCACGACAACCAGAACGACCCATCGAAACTTTTTCACTCCAAtctc                                       >  1:7770300/1‑140 (MQ=255)

                                                                                                   tGGTTAATGGCACAAATTCCGCTGAAAAATTGTACATCCTGTATCGCACATCATGTTGAATACCTGCGCCCAAAGCAGCAAGCAAGCCAACACCACGACAACCAGAACGACCCATCGAAACTTTTTCACTCCAATCTCCg                                     <  2:4806733/140‑1 (MQ=255)

                                                                                                   tGGTTAATGGCACAAATTACGATGAAAAATTGTACATCCTGTATCTCACTTCTTGTTGAATACCTGCGCCCAAAGCAGCAAGCAAGCCAACACCACGACATCCAGAACGACCCTTCGAAACTTTTTCACTCCAATCTCCg                                     <  2:3214431/140‑1 (MQ=255)

                                                                                                     gagaaTGGCACAAATTCCGCTGAAAAATTGTACATCCTGTATCGCACATCATGTTGAATACCTGCGCCCAAAGCAGCAAGCAAGCCAACACCACGACAACCAGAACGACCCATCGAAACTTTTTCACTCCAATCTCCGtt                                   <  2:7910107/137‑1 (MQ=255)

                                                                                                      ttAATGGCACAAATTCCGCTGAAAAATTGTACATCCTGTATCGCACATCATGTTGAATACCTGCGCCCAAAGCagcaag                                                                                               >  1:4654935/1‑79 (MQ=255)

                                                                                                      ttAATGGCACAAATTCCGCTGAAAAATTGTACATCCTGTATCGCACATCATGTTGAATACCTGCGCCCAAAGCAGCAAGCAAGCCAAcaccac                                                                                 >  2:4078806/1‑93 (MQ=255)

                                                                                                      ttAATGGCACAAATTCCGCTGAAAAATTGTACATCCTGTATCGCACATCATGTTGAATACCTGCGCCCAAAGCAGCAAGCAAGCCAACACCACGACAACCAGAACGACCCATCGAAACTTTTTCACTCCAATCTCCGttt                                  >  1:1202222/1‑140 (MQ=255)

                                                                                                      ttAATGGCACAAATTCCGCTGAAAAATTGTACATCCTGTATCGCACATCATGTTGAATACCTGCGCCCAAAGCAGCAAGCAAGCCAACACCACGACAACCAGAACGACCCATCGAAACTTTTTCACTCCAATCTCCGttt                                  >  1:6457076/1‑140 (MQ=255)

                                                                                                      atAATGGCACAAATTCCGCTGAAAAAGAGTACATCCTGTAGCGCACCTCATGTTGAATACCTGAGAGCAACGCAGCAAGCAAGCCAAAACCAAGTCAAGCCGACCGAGCGCTCGAGGCTGATGCTCTCCTATCTCCgggc                                  >  2:6965899/2‑137 (MQ=255)

                                                                                                         aTGGCACAAATTCCGCTGAAAAATTGTACATCCTGTATCGCACATCATGTTGAATACCTGCGCCCAAAGCAGCAAGCAAGCCAACACCACGACAACCAGAACGACCCATCGAAACTTTTTCACTCCAATCTCCGTTTCac                               <  1:9344602/140‑1 (MQ=255)

                                                                                                         aTGGCACAAATTCCGCTGAAAAATTGTACATCCTGTATCGCACATCATGTTGAATACCTGCGCCCAAAGCAGCAAGCAAGCCAACACCACGACAACCAGAACGACCCATCGAAACTTTTTCACTCCAATCTCCGTTTCac                               <  2:8819189/140‑1 (MQ=255)

                                                                                                          tGGCACAAATTCCGCTGAAAAATTGTACATCCTGTATCGCACATCATGTTGAATACCTGCGCCCAAAGCAGCAAGCAAGCCAACACCACGACAACCAGAACGACCCATCGAAACTTTTTCACTCCAATCTCCGTTTCacc                              <  2:3321753/140‑1 (MQ=255)

                                                                                                          tGGCACAAATTCCGCTGAAAAATTGTACATCCTGTATCGCACATCATGTTGAATACCTGCGCCCAAAGCAGCAAGCAAGCCAACACCACGACAACCAGAACGACCCATCGAAACTTTTTCACTCCAATCTCCGTTTCacc                              <  1:5431698/140‑1 (MQ=255)

                                                                                                          tGGCACAAATTCCGCTGAAAAATTGTACATCCTGTATCGCACATCATGTTGAATACCTGCGCCCAAAGCAGCAAGCAAGCCAACACCACGACAACCAGAACGACCCATCGAAACTTTTTCACTCCAATCTCCGTTTCacc                              >  1:4506655/1‑140 (MQ=255)

                                                                                                           ggCACAAATTCCGCTGAAAAATTGTACATCCTGTATCGCACATCATGTTGAATACCTGCGCCCAAAGCAGCAAGCAAGCCAACACCACGACAACCAGAACGACCCATCGAAACTTTTTCACTCCAATCTCCGTTTCacct                             >  1:4837216/1‑140 (MQ=255)

                                                                                                           ggCACAAATTCCGCTGAAAAATTGTACATCCTGTATCGCACATCATGTTGAATACCTGCGCCCAAAGCAGCAAGCAAGCCAACACCACGACAACCAGAACGACCCATCGAAACTTTTTCACTCCAATCTCCGTTTCacct                             >  1:3662157/1‑140 (MQ=255)

                                                                                                           ggCACAAATTCCGCTGAAAAATTGTACATCCTGTATCGCACATCATGTTGAATACCTGCGCCCAAAGCAGCAAGCAAGCCAACACCACGACAACCAGAACGACCCATCGAAACTTTTTCACTCCAATCTCCGTTTCacct                             >  2:6488919/1‑140 (MQ=255)

                                                                                                             cacaAATTCCGCTGAAAAATTGTACATCCTGTATCGCACATCATGTTGAATACCTGCGCCCAAAGCAGCAAGCAAGCCAACACCACGACAACCAGAACGACCCATCGAAACTTTTTCACTCCAATCTCCGTTTCacctac                           >  1:750401/1‑140 (MQ=255)

                                                                                                             cacaAATTCCGCTGAAAAATTGTACATCCTGTATCGCACATCATGTTGAATACCTGCGCCCAAAGCAGCAAGCAAGCCAACACCACGACAACCAGAACGACCCATCGAAACTTTTTCACTCCAATCTCCGTTTCacctac                           >  1:6964669/1‑140 (MQ=255)

                                                                                                             cacaAATTCCGCTGAAAAATTGTACATCCTGTATCGCACATCATGTTGAATACCTGCGCCCAAAGCAGCAAGCAAGCCAACACCACGACAACCAGAACGACCCATCGAAACTTTTTCACTCCAATCTCCGTTTCacctac                           >  1:261683/1‑140 (MQ=255)

                                                                                                               caAATTCCGCTGAAAAATTGTACATCCTGTATCGCACATCATGTTGAATACCTGCGCCCAAAGCAGCAAGCAAGCCAACACCACGACAACCAGAACGACCCATCGAAACTTTTTCACTCCAATCTCCGTTTCacctacct                         >  2:7299483/1‑140 (MQ=255)

                                                                                                               caAATTCCGCTGAAAAATTGTACATCCTGTATCGCACATCATGTTGAATACCTGCGCCCAAAGCAGCAAGCAAGCCAACACCACGACAACCAGAACGACCCATCGAAACTTTTTCACTCCAATCTCCGTTTCacctacct                         >  2:4252342/1‑140 (MQ=255)

                                                                                                                  aTTCCGCTGAAAAATTGTACATCCTGTATCGCACATCATGTTGAATACCTGCGCCCAAAGCAGCAAGCAAGCCAACACCACGACAACCAGAACGACCCATCGAAACTTTTTCACTCCAATCTCCGTTTCACCTACCTTAt                      <  1:8122492/140‑1 (MQ=255)

                                                                                                                  aTTCCGCTGAAAAATTGTACATCCTGTATCGCACATCATGTTGAATACCTGCGCCCAAAGCAGCAAGCAAGCCAACACCACGACAACCAGAACGACCCATCGAAACTTTTTCACTCCAATCTCCGTTTCACCTACCTTAt                      <  1:6091847/140‑1 (MQ=255)

                                                                                                                    tCCGCTGAAAAATTGTACATCCTGTATCGCACATCATGTTGAATACCTGCGCCCAAAGCAGCAAGCAAGCCAACACCACGACAACCAGAACGACCCATCGAAACTTTTTCACTCCAATCTCCGTTTCACCTACCTTATGt                    >  2:9414510/1‑140 (MQ=255)

                                                                                                                    tCCGCTGAAAAATTGTACATCCTGTATCGCACATCATGTTGAATACCTGCGCCCAAAGCAGCAAGCAAGCCAACACCACGACAACCAGAACGACCCATCGAAACTTTTTCACTCCAATCTCCGTTTCACCTACCGTATGt                    >  1:891706/1‑140 (MQ=255)

                                                                                                                      cgcgGAAAAATTGTACATCCTGTATCGCACATCATGTTGAATACCTGCGCCCAAAGCAGCAAGCAAGCCAACACCACGACAACCAGAACGACCCATCGAAACTTTTTCACTCCAATCTCCGTTTCACCTACCTTATGTCa                  <  1:9491502/140‑1 (MQ=255)

                                                                                                                      cGCTGAAAAATTGTACATCCTGTATCGCACATCATGTTGAATACCTGCGCCCAAAGCAGCAAGCAAGCCAACACCACGACAACCAGAACGACCCATCGAAACTTTTTCACTCCAATCTCCGTTTCACCTACCTTATGTCa                  <  1:2070985/140‑1 (MQ=255)

                                                                                                                      cGCTGAAAAATTGTACATCCTGTATCGCACATCATGTTGAATACCTGCGCCCAAAGCAGCAAGCAAGCCAACACCACGACAACAAGAACGACCCATCGAAACTTATTCACTCCAATCTCCGTTTCACCTACCTTATGTCa                  >  2:7586229/1‑140 (MQ=255)

                                                                                                                           aaaaaTTGTACATCCTGTATCGCACATCATGTTGAATACCTGCGCCCAAAGCAGCAAGCAAGCCAACACCACGACAACCAGAACGACCCATCGAAACTTTTTCACTCCAATCTCCGTTTCACCTACCTTATGTCATTaaa             <  2:1448733/140‑1 (MQ=255)

                                                                                                                           aaaaaTTGTACATCCTGTATCGCACATCATGTTGAATACCTGCGCCCAAAGCAGCAAGCAAGCCAACACCACGACAACCAGAACGACCCATCGAAACTTTTTCACTCCAATCTCCGTTTCACCTACCTTATGTCATTaaa             <  1:2206958/140‑1 (MQ=255)

                                                                                                                               aTTGTACCGCCTGTATCGCACATCCTGGTGAAGACAGGCGCCCACAGCAGCAACCAAGCCACCACCCCGACAACCACACCGACCCATCGAAACTTATTCACTCCAAGCTCCGGTTCACCTACCTTATGTCATTAAACtcg         <  2:6647124/140‑3 (MQ=255)

                                                                                                                                ttGTACATCCTGTATCGCACATCATGTTGAATACCTGCGCCCAAAGCAGCAAGCAAGCCAACACCACGACAACCAGAACGACCCATCGAAACTTTTTCACTCCAATCTCCGTTTCACCTACCTTATGTCATTAAACTAGc        <  1:5201268/140‑1 (MQ=255)

                                                                                                                                 tGTACATCCTGTATCGCACATCATGTTGAATACCTGCGCCCAAAGCAGCAAGCAAGCCAACACCACGACAACCAGAACGACCCATCGAAACTTTTTCACTCCAATCTCCGTTTCACCTACCTTATGTCATTAAACTAGCa       >  1:628723/1‑140 (MQ=255)

                                                                                                                                   tACATCCTGTATCGCACATCATGTTGAATACCTGCGCCCAAAGCAGCAAGCAAGCCAACACCACGACAACCAGAACGACCCATCGAAACTTTTTCACTCCAATCTCCGTTTCACCTACCTTATGTCATTAAACTAGCATg     >  1:2891674/1‑140 (MQ=255)

                                                                                                                                     cATCCTGTATCGCACATCATGTTGAATACCTGCGCCCAAAGCAGCAAGCAAGCCAACACCACGACAACCAGAACGACCCATCGAAACTTTTTCACTCCAATCTCCGTTTCACCTACCTTATGTCATTAAACTAGCATGCg   <  1:1015355/140‑1 (MQ=255)

                                                                                                                                      aTCCTGTATCGCACATCATGTTGAATACCTGCGCCCAAAGCAGCAAGCAAGCCAACACCACGACAACCAGAACGACCCATCGAAACTTTTTCACTCCAATCTCCGTTTCACCTACCTTATGTCATTAAACTAGCATGCgt  <  2:3408874/140‑1 (MQ=255)

TTCGTTCATTTCACCACCTCTGAGTAGAGGCGCTATTCTACCACTGCTGGAGAGGAAGAAAATCTAGTGCTGAAAAAATGATATCACCACGGGATAAACTGGTTAATGGCACAAATTCCGCTGAAAAATTGTACATCCTG‑ATCGCACATCATGTTGAATACCTGCGCCCAAAGCAGCAAGCAAGCCAACACCACGACAACCAGAACGACCCATCGAAACTTTTTCACTCCAATCTCCGTTTCACCTACCTTATGTCATTAAACTAGCATGCGT  >  NZ_CP025268/1913353‑1913625

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`