BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP025268	2,292,596	(C)_6→7	pseudogene (361/2531 nt)	yejO ←	autotransporter YejO

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP025268	2,292,590	1	.	C	96.5%	1611.2 / 46.7	495	pseudogene (367/2531 nt)	yejO	autotransporter YejO
Reads supporting (aligned to +/- strand): ref base . (9/8); new base C (186/290); total (196/299)
Fisher's exact test for biased strand distribution p-value = 3.14e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 538 total aligned reads displayed.

ACCGAGACGGTTTGTTCCGAATACCTCCATGGCCCGCGTGGTTGTTTTAATAGCACCGCCTGCTTTCTGATCTACCGCAAATGCAAATCCCCCCTCTTTAACCTCCAGTAGTCCTCCCTTTTCGATAGCGACAAAGAC‑GG‑C‑C‑CCCC‑ATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCTGCGCCATAAACCTGTAGCACACCATTATTGAGCGTAGTCTGGTTTGCCGCGCCACCCATTTGAACAATTTGATAACCGT  >  NZ_CP025268/2292451‑2292724

aCCGAGACGGTTTGTTCCGAATACCTCCATGGCCCGCGTGGTTGTTTTAATAGCACCGCCTGCTTTCTGATCTACCGCAAATGCAAATCCCCCCTCTTTAACCTCCAGTAGTCCTCCCTTTTCGATAGCGACAAAGAC‑gg                                                                                                                                            >  2:2609585/1‑140 (MQ=255)

aCCGAGACGGTTTGTTCCGAATACCTCCATGGCCCGCGTGGTTGTTTTAATAGCACCGCCTGCTTTCTGATCTACCGCAAATGCAAATCCCCCCTCTTTAACCTCCAGTAGTCCTCCCTTTTCGATAGCGACAAAGAC‑gg                                                                                                                                            <  1:97352/140‑1 (MQ=255)

aCCGAGACGGTTTGTTCCGAATACCTCCATGGCCCGCGTGGTTGTTTTAATAGCACCGCCTGCTTTCTGATCTACCGCAAATGCAAATCCCCCCTCTTTAACCTCCAGTAGTCCTCCCTTTTCGATAGCGACAAAGAC‑gg                                                                                                                                            >  2:6095374/1‑140 (MQ=255)

    agaCGGTTTGTTCCGAATACCTCCATGGCCCGCGTGGTTGTTTTAATAGCACCGCCTGCTTTCTGATCTACCGCAAATGCAAATCCCCCCTCTTTAACCTCCAGTAGTCCTCCCTTTTCGATAGCGACAAAGAC‑GG‑c‑c‑cc                                                                                                                                     >  1:4689837/1‑140 (MQ=255)

          tttGTTCCGAATACCTCCATGGCCCGCGTGGTTGTTTTAATAGCACCGCCTGCTTTCTGATCTACCGCAAATGCAAATCCCCCCTCTTTAACCTCCAGTCGTCCTCCCTTTTCGATAGCGACAAAGAC‑GG‑c‑c‑cccccatc                                                                                                                               >  1:1372015/1‑136 (MQ=255)

          tttGTTCCGAATACCTCCATGGCCCGCGTGGTTGTTTTAATAGCACCGCCTGCTTTCTGATCTACCGCAAATGCAAATCCCCCCTCTTTAACCTCCAGTAGTCCTCCCTTTTCGATAGCGACAAAGAC‑GG‑c‑c‑cccccatc                                                                                                                               >  2:6535941/1‑136 (MQ=255)

          tttGTTCCGAATACCTCCATGGCCCGCGTGGTTGTTTTAATAGCACCGCCTGCTTTCTGATCTACCGCAAATGCAAATCCCCCCTCTTTAACCTCCAGTAGTCATCCCTTTTCGATAGCGACAAAGAC‑GG‑c‑c‑cccccatc                                                                                                                               >  1:8596181/1‑136 (MQ=255)

           ttGTTCCGAATACCTCCATGGCCCGCGTGGTTGTTTTAATAGCACCGCCTGCTTTCTGATCTACCGCAAATGCAAATCCCCCCTCTTTAACCTCCAGTAGTCCTCCCTTTTCGATAGCGACAAAGAC‑GG‑c‑c‑cccccatct                                                                                                                              <  1:7757758/140‑6 (MQ=255)

               tCCGAATACCTCCATGGCCCGCGTGGTTGTTTTCATAGCACCGCCTGCTTTCTGATCTACCGCCAAGGCAATTCCCCCATCGTTGACGTACTGTAGTCCTCCCATTTCGAGCGCGAGAAAGTC‑GG‑C‑CGCCCC‑CTCttgat                                                                                                                          >  2:4859320/1‑137 (MQ=255)

                   aaTACCTCCATGGCCCGCGTGGTTGTTTTAATAGCACCGCCTGCTTTCTGATCTACCGCAAATGCAAATCCCCCCTCTTTAACCTCCAGTAGTCCTCCCTTTTCGATAGCGACAAAGAC‑GGCC‑C‑CCCC‑ATCTTTTTCAAc                                                                                                                      >  2:2757141/1‑140 (MQ=255)

                        cTCCATGGCCCGCGTGGTTGTTTTAATAGCACCGCCTGCTTTCTGATCTACCGCAAATGCAAATCCCCCCTCTTTAACCTCCAGTAGTCCTCCCTTTTCGATAGCGACAAAGAC‑GGCC‑C‑CCCC‑ATCTTTTTCAACGATTa                                                                                                                 >  1:6759562/1‑140 (MQ=255)

                        cTCCATGGCCCGCGTGGTTGTTTTAATAGCACCGCCTGCTTTCTGATCTACCGCAAATGCAAATCCCCCCTCTTTAACCTCCAGTAGTCCTCCCTTTTCGATAGCGACAAAGAC‑GGCC‑C‑CCCC‑ATCTTTTTCAACGATTa                                                                                                                 >  2:4988890/1‑140 (MQ=255)

                        cTCCATGGCCCGCGTGGTTGTTTTAATAGCACCGCCTGCTTTCTGAGCTAGCGCAAATTCAATTCCCCCCTCTATAACCTCAAGTAGTCCTCCGTTGTCGATGTCGACATAGAC‑GG‑C‑CACCCC‑AGCTTTTTCCACGAttt                                                                                                                 >  2:1262272/1‑139 (MQ=255)

                           cATGGCCCGCGTGGTTGTTTTAATAGCACCGCCTGCTTTCTGATCTACCGCACATGCAAATCCCCCCTCTTTAACCTCCAGTAGTCCTCCCTTTTCGATAGCGACAAAGAC‑GGCC‑C‑CCCC‑ATCTTTTTCAACGATTAAgc                                                                                                              >  1:3321151/1‑140 (MQ=255)

                              ggCCCGCGTGGTTGTTTTAATAGCACCGCCTGCTTTCTGATCTACCGCAAATGCAAATCCCCCCTCTTTAACCTCCAGTAGTCCTCCCTTTTCGATAGCGACAAAGAC‑GGCC‑C‑CCCC‑ATCTTTTTCAACGATTAAGcgcc                                                                                                           <  2:8172077/140‑1 (MQ=255)

                              ggCCCGCGTGGTTGTTTTAATAGCACCGCCTGCTTTCTGATCTACCGCAAATGCAAATCCCCCCTCTTTAACCTCCAGTAGTCCTCCCTTTTCGATAGCGACAAAGAC‑GGCC‑C‑CCCC‑ATCTTTGTCAACGATTAAGcgcc                                                                                                           <  2:8799820/140‑1 (MQ=255)

                              cgCCCGCGTGGTCGCAATACTCTCACCGCCTGCCAGCATATCAACCCCAAAGGCACATCCCCCCTCTATAACCTCCAGTACCCCTCCCTTCTCGATAGCGACAAAGAC‑GGCC‑C‑CCCC‑AACTGGTTCAACGATTAAGcgcc                                                                                                           <  2:5933182/139‑1 (MQ=255)

                                cccGCGTGGTTGTTTTAATAGCACCGCCTGCTTTCTGATCTACCGCAAATGCAAATCCCCCCTCTTTAACCTCCAGTAGTCCTCCCTTTTCGATAGCGACAAAGAC‑GGCC‑C‑CCCC‑ATCTTTTTCAACGATTAAGcgcccg                                                                                                         >  1:7262020/1‑140 (MQ=255)

                                    cgTGGTTGTTTTAATAGCACCGCCTGCTTTCTGATCTACCGCAAATGCAAATCCCCCCTCTTTAACCTCCAGTAGTCCTCCCTTTTCGATAGCGACAAAGAC‑GGCC‑C‑CCCC‑ATCTTTTTCAACGATTAAGCGCCCGCCtt                                                                                                     >  1:2461896/1‑140 (MQ=255)

                                    cgTGGTTGTTTTAATAGCACCGCCTGCTTTCTGATCTACCGCAAATGCAAATCCCCCCTCTTTAACCTCCAGTAGTCCTCCCTTTTCGATAGCGACAAAGAC‑GGCC‑C‑CCCC‑ATCTTTTTCAACGATTAAGCGCCCGCCtt                                                                                                     >  2:7585605/1‑140 (MQ=255)

                                    cgTGGTTGTTTTAATAGCACCGCCTGCTTTCTGATCTACCGCAAATGCAAATCCCCCCTCTTTAACCTCCAGTAGTCCTCCCTTTTCGATAGCGACAAAGAC‑GGCC‑C‑CCCC‑ATCTTTTTCAACGATTAAGCGCCCGCCtt                                                                                                     >  2:7952475/1‑140 (MQ=255)

                                    cgTGGTTGTTTTAATAGCACCGCCTGCTTTCTGATCTACCGCAAATGCAAATCCCCCCTCTTTAACCTCCAGTAGTCCTCCCTTTTCGATAGCGACAAAGAC‑GGCC‑C‑CCCC‑ATCTTTTTCAACGATTAAGCGCCCGCCtg                                                                                                     >  1:2376138/1‑139 (MQ=255)

                                    cgTGGTTGTTTTAATAGCACCGCCTGCTTTCTGATCTACCGCAAATGCAAATCCCCCCTCTTTAACCTCCAGTAGTCCTCCCTTTTCGATAGCGACAAAGAC‑GGAC‑C‑CCCC‑ATCTTTTTCAACGATTAAGCGCCCGCCtt                                                                                                     >  1:3474592/1‑140 (MQ=255)

                                    cgTGGTTGTTTTAATAGCACCGCCTGCTTTCTGATCTACCGCAAATGCAAATCCCCCCTCTTTAACCTCCAGTAGTCCTCCCTTTTCGATAGCGACAAAGAC‑GG‑CAC‑CCCC‑ATCTTTTTCAACGATTAAGCGCCCGCCtt                                                                                                     >  2:5498988/1‑140 (MQ=255)

                                     gTGGTTGTTTTAATAGCACCGCCTGCTTTCTGATCTACCGCAAATGCAAATCCCCCCTCTTTAACCTCCAGTAGTCCTCCCTTTTCGATAGCGACAAAGAC‑GGCC‑C‑CCCC‑ATCTTTTTCAACGATTAAGCGCCCGCCttt                                                                                                    <  1:5357458/140‑1 (MQ=255)

                                       ggTTGTTTTAATAGCACCGCCTGCTTTCTGATCTACCGCAAATGCAAATCCCCCCTCTTTCACCTCCAGTAGTCCTCCCTTTTCGATAGCGACAAAGAC‑GGCC‑C‑CCCC‑ATCTTTTTCAACGATTAAGCGCCCGCCTTTaa                                                                                                  <  1:8393073/140‑1 (MQ=255)

                                        gttgttTTAATAGCACCGCCTGCTTTCTGATCTACCGCAAATGCAACTCCCCCCTCTTTAACCTCCAGTAGTCCTCCCTTTTCGATAGCGACAAAGAC‑GGCC‑C‑CCCC‑ATCTTTTTCAACGATTAAGCGCCCGCCTTTAAt                                                                                                 <  1:5921400/140‑1 (MQ=255)

                                                   aGCACCGCCTGCTTTCTGATCTACCGCAAATGCAAATCCCCCCTCTTTAACCTCCAGTAGTCCTCCCTTTTCGATAGCGACAAAGAC‑GGCC‑C‑CCCC‑ATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCAt                                                                                      >  2:4896513/1‑140 (MQ=255)

                                                   aGCACCGCCTGCTTTCTGATCTACCGCAAATGCAAATCCCCCCTCGTTAACCTCCAGTAGTCCTCCCTTTTCGATAGCGACAAAGAC‑GGCC‑C‑CCCC‑ATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCAt                                                                                      >  1:4581816/1‑140 (MQ=255)

                                                     cACCGCCTGCTTTCTGATCTACCGCAAATGCAAATCCCCCCTCTTTAACCTCCAGTAGTCCTCCCTTTTCGATAGCGACAAAGAC‑GGCC‑C‑CCCC‑ATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTc                                                                                    >  1:202239/1‑140 (MQ=255)

                                                       ccGCCTGCTTTCTGATCTACCGCAAATGCAAATCCCCCCTCTTTAACCTCCAGTAGTCCTCCCTGTTCGATAGCGACAAAGAC‑GGCC‑C‑CCCC‑ATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTCTCATTCGc                                                                                  >  2:220300/1‑140 (MQ=255)

                                                        cGCCTGCTTTCTGATCTACCGCAAATGCAAATCCCCCCTCTTTAACCTCCAGTAGTCCTCCCTTTTCGATAGCGACAAAGAC‑GGCC‑C‑CCCC‑ATCTTTTTCAACGATTAAGCGCCCGCCTTTATTCGTGGTATCAATCGCt                                                                                 >  2:1593380/1‑140 (MQ=255)

                                                        cGCCTGCTTTCTGATCTACCGCAAATGCAAATCCCCCCTCTTTAACCTCCAGTAGTCCTCCCTTTTCGATAGCGACAAAGAC‑GGCC‑C‑CCCC‑ATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCt                                                                                 <  2:1724739/140‑1 (MQ=255)

                                                         gCCTGCTTTCTGATCTACCGCAAATGCAAATCCCCCCTCTTTAACCTCCAGTAGTCCTCCCTTTTCGATAGCGACAAAGAC‑GGCC‑C‑CCCC‑ATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCTg                                                                                <  1:4349051/140‑1 (MQ=255)

                                                          ccTGCTTTCTGATCTACCGCAAATGCAAATCCCCCCTCTTTAACCTCCAGTAGTCCTCCCTTTTCGATAGCGACAAAGAC‑GGCC‑C‑CCCC‑ATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCTgc                                                                               >  1:9432183/1‑140 (MQ=255)

                                                          ccTGCTTTCTGATCTACCGCAAATGCAAATCCCCCCTCTTTAACCTCCAGTAGTCCTCCCTTTTCGATAGCGACAAAGAC‑GGCC‑C‑CCCC‑ATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCTgc                                                                               >  2:1491518/1‑140 (MQ=255)

                                                          ccTGCTTTCTGATCTACCGCAAATGCAAATCCCCCCTCTTTAACCTCCAGTAGTCCTCCCTGTTCGATAGCGACAAAGAC‑GGCC‑C‑CCCC‑ATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCTgc                                                                               >  1:3009802/1‑140 (MQ=255)

                                                          ccTGCTTTCTGATCTACCGCAAATGCAAATCCCCCCTCTTTAACCTCCAGTAGGCCTCCCTTTTCGATAGCGACAAAGAC‑GGCC‑C‑CCCC‑ATCTTTTTCAACGATGAAGCACCCGCCTTTAATCGTGGTAGCATTCGCTgc                                                                               >  1:540313/1‑140 (MQ=255)

                                                              cTTTCTGATCTACCGCAAATGCAATTCCCCCCTCTTTAACCTCCAGTAGTCCTCCCTTTTCGATAGCGACAAAGAC‑GGCC‑C‑CCCC‑ATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCTGCGCCa                                                                           >  1:3897648/1‑140 (MQ=255)

                                                              cTTTCTGATCTACCGCAAATGCAAATCCCCCCTCTTTCACCTCCAGTAGTCCTCCCTTTTCGATAGCGACAAAGAC‑GGCC‑C‑CCCC‑ATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCTGCGCCa                                                                           <  2:9504343/140‑1 (MQ=255)

                                                                 tCTGATCTACCGCAACTGCAAATCCCCCNTCTGTCACCTCCCGTAGTCCTCCCTTTTCGATAGCGACAAAGACTGC‑C‑C‑CCCC‑ATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCTGCGCCATaa                                                                        <  1:5571826/140‑1 (MQ=255)

                                                                   tGATCTCCCGCACATGCAACGCCCCCCGCTTTCACCTCCAGTAGTCCGCCCTCTTCGAGCGCGACAAAGAC‑GGCC‑C‑CCCC‑AGCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCTGCGCCATAAAc                                                                      <  2:5128356/140‑1 (MQ=255)

                                                                   tGATCTACCGCAAATGCAAATCCCCCCTCTTTAACCTCCAGTAGTCCTCCCTTTTCGATAGCGACAAAGAC‑GGCC‑C‑CCCC‑ATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCTGCGCCATAAAc                                                                      >  1:5741241/1‑140 (MQ=255)

                                                                    gATCTACCGCAAATGCAAATCCCCCCTCTTTAACCTCCAGTAGTCCTCCCTTTTCGATAGCGACAAAGAC‑GGCC‑C‑CCCC‑ATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCTGCGCCATAAAcc                                                                     >  1:2833016/1‑140 (MQ=255)

                                                                      tCTACCGCAAATGCAAATCCCCCCTCTTTAACCTCCAGTAGTCCTCCCTTTTCGAGAGCGACAAAGAC‑GGCC‑C‑CCCC‑ATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCTGCGCCATAAACCTg                                                                   <  2:2042444/140‑1 (MQ=255)

                                                                         aCCGCAAATGCAAATCCCCCCTCTTTAACCTCCAGTAGTCCTCCCTTTTCGATAGCGACAAAGAC‑GGCC‑C‑CCCC‑ATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCTGCGCCATAAACCTGTAg                                                                <  1:1203956/140‑1 (MQ=255)

                                                                         aCCGCAAATGCAAATCCCCCCTCTTTAACCTCCAGTAGTCCTCCCTTTTCGATAGCGACAAAGAC‑GGCC‑C‑CCCC‑ATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCTGCGCCATAAACCTGTAg                                                                <  1:3501474/140‑1 (MQ=255)

                                                                         aCCGCAAATGCAAATCCCCCCTCTTTAACCTCCAGTAGTCCTCCCTTTTCGATAGCGACAAAGAC‑GGCC‑C‑CCCC‑ATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCTGCGCCATAAACCTGTAg                                                                <  1:1517736/140‑1 (MQ=255)

                                                                           cgaaaattcaaatCCCCCCTCTTTAACCTCCAGTAGTCCTCCCTTGTCGATAGCGACAAAGAC‑GG‑C‑C‑CCCCAATCTTTTTCAACTATTAAGCGCCCGCATTTAATCGTGGTATCATTCGCTCCGCCATAACCCTTTAGcc                                                              <  2:4185150/137‑2 (MQ=255)

                                                                           cGCAAATGCAACTCCCCCCTCTTTAACCTCCAGTAGTCCTCCCTTTTCGATAGCGACAAAGAC‑GGCC‑C‑CCCC‑ATCTTTTTCAACGATTATGCGCCCGCCTTTAATCGTGGTATCATTCGCTGCGCCATAAACCTGTAGca                                                              <  2:7198588/140‑1 (MQ=255)

                                                                           cGCAAATGCAAATCCCCCCTCTTTCACCTCCAGTAGTCCTCCCTTTTCGATAGCGACAAAGAC‑GGCC‑C‑CCCC‑ATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCTGCGCCATAAACCTGTAGca                                                              <  2:704175/140‑1 (MQ=255)

                                                                           cGCAAATGCAAATCCCCCCTCTTTAACCTCCAGTAGTCCTCCCTTTTCGATAGCGACAAAGAC‑GGCC‑C‑CCCC‑ATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCTGCGCCATAAACCTGTAGca                                                              <  1:7552622/140‑1 (MQ=255)

                                                                           cGCAAATGCAAATCCCCCCTCTTTAACCTCCAGTAGTCCTCCCTTTTCGATAGCGACAAAGAC‑GGCC‑C‑CCCC‑ATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCTGCGCCATAAACCTGTAGca                                                              <  2:3835254/140‑1 (MQ=255)

                                                                           cGCAAATGCAAATCCCCCCTCTTTAACCTCCAGTAGTCCTCCCTTTTCGATAGCGACAAAGAC‑GGCC‑C‑CCCC‑ATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCTGCGCCATAAACCTGTAGca                                                              <  1:3763767/140‑1 (MQ=255)

                                                                           cGCAAATGCAAATCCCCCCTCTTTAACCTCCAGTAGTCCTCCCTTTTCGATAGCGACAAAGAC‑GGCC‑C‑CCCC‑ATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCTGCGCCATAAACCTGTAGca                                                              <  2:3418918/140‑1 (MQ=255)

                                                                           cGCAAATGCAAATCCCCCCTCTTTAACCTCCAGTAGTCCTCCCTTTTCGATAGCGACAAAGAC‑GGCC‑C‑CCCC‑ATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCTGCGCCATAAACCTGTAGca                                                              <  2:3261866/140‑1 (MQ=255)

                                                                           cGCAAATGCAAATCCCCCCTCTTTAACCTCCAGTAGTCCTCCCTTTTCGATAGCGACAAAGAC‑GGCC‑C‑CCCC‑ATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCTGCGCCATAAACCTGTAGca                                                              <  1:4507081/140‑1 (MQ=255)

                                                                           cGCAAAGGCAAATCCCCCCTCTTTAACCTCCAGTAGTCCTCCCTTTTCGAGAGCGACAAAGAC‑GGCC‑C‑CCCC‑ATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCTGCGCCATAAACCTGTAGca                                                              <  2:6832946/140‑1 (MQ=255)

                                                                             caaatgcaaatCCCCCCTCTTTAACCTCCAGTAGTCCTCCCTTTTCGATAGCGACAAAGAC‑GGCC‑C‑CCCC‑ATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCTGCGCCATAAACCTGTAGcaca                                                            >  1:178875/1‑140 (MQ=255)

                                                                               aatgcaaatCCCCCCTCTTTAACCTCCAGTAGTCCTCCCTTTTCGATAGCGACAAAGAC‑GGCC‑C‑CCCC‑ATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCTGCGCCATAAACCTGTAGCACAcc                                                          >  1:669502/1‑140 (MQ=255)

                                                                                atgcaaatCCCCCCTCTTTAACCTCCAGTAGTCCTCCCTTTTCGATAGCGACAAAGAC‑GGCC‑C‑CCCC‑ATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCTGCGCCATAAACCTGTAGCACACCa                                                         <  1:892975/140‑1 (MQ=255)

                                                                                atgcaaatCCCCCCTCTTTAACCTCCAGTAGTCCTCCCTTTTCGATAGCGACAAAGAC‑GGCC‑C‑CCCC‑ATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCTGCGCCATAAACCTGTAGCACACCa                                                         <  2:4760832/140‑1 (MQ=255)

                                                                                atgcaaatCCCCCCTCCTTACCCTCCAGTAGTCCTCCCTTTTCGATAGCGACAAAGAC‑GGCC‑C‑CCCC‑ATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCTGCGCCATAAACCTGTAGCACACCa                                                         <  2:9028545/140‑1 (MQ=255)

                                                                                       tCCCCCCTCTTTAACCTCCAGTAGTCCTCCCTTTTCGATAGCGACAAAGAC‑GGCC‑C‑CCCC‑ATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCTGCGCCATAAACCTGTAGCACACCATTATTGa                                                  <  1:2936884/140‑1 (MQ=255)

                                                                                        ccccccTCTTTAACCTCCAGTAGTCCTCCCTTTTCGATAGCGACAAAGAC‑GGCC‑C‑CCCC‑ATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCTGCGCCATAAACCTGTAGCACACCATTATTGAg                                                 <  1:4878129/140‑1 (MQ=255)

                                                                                          ccccTCTTTACCCTCCAGTAGTCCTCCCTTTTCGATAGCGACAAAGAC‑GGCC‑C‑CCCC‑ATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCTGCGCCATAAACCTGTAGCACACCATTATTGAGCg                                               <  2:4022375/140‑1 (MQ=255)

                                                                                          ccccTCTTTAACCTCCAGTAGTCCTCCCATTGCGATAGCGACACAGAC‑GGCC‑C‑CCCC‑ATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATGCGCTGCGCCATAAACCTGTAGCACACCATTCTTGAGCg                                               <  2:5632290/140‑1 (MQ=255)

                                                                                            ccTCTTTAACCTCCAGTAGTCCTCCCTTTTCGATAGCGACAAAGAC‑GGCC‑C‑CCCC‑ATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCTGCGCCATAAACCTGTAGCACACCATTATTGAGCGTa                                             >  2:6401988/1‑140 (MQ=255)

                                                                                            ccTCTTTAACCTCCAGTAGTCCTCCCTTTTCGATAGCGACAAAGAC‑GGCC‑C‑CCCC‑ATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCTGCGCCATAAACCTGTAGCACACCATTATTGAGCGTa                                             >  2:9267557/1‑140 (MQ=255)

                                                                                                    aCCTCCAGTAGTCCTCCCTTTTCGATAGCGACAAAGAC‑GGCC‑C‑CCCC‑ATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCTGCGCCATAAACCTGTAGCACACCATTATTGAGCGTAGTCTGGtt                                     >  2:1122023/1‑140 (MQ=255)

                                                                                                      cTCCAGTAGTCCTCCCTTTTCGATAGCGACAAAGAC‑GGCC‑C‑CCCC‑ATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCTGCGCCATAAACCTGTAGCACACCATTATTGAGCGTAGTCTGGTTTg                                   <  2:6001445/140‑1 (MQ=255)

                                                                                                         cAGTAGTCCTCCCTTTTCGATAGCGACAAAGAC‑GGCC‑C‑CCCC‑ATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCTGCGCCATAAACCTGTAGCACACCATTATTGAGCGTAGTCTGGTTTGCcg                                <  2:4458081/140‑1 (MQ=255)

                                                                                                         cAGTAGTCCTCCCTTTTCGATAGCGACAAAGAC‑GGCC‑C‑CCCC‑ATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCTGCGCCATAAACCTGTAGCACACCATTATTGAGCGTAGTCTGGTTTGCcg                                >  2:834276/1‑140 (MQ=255)

                                                                                                          agtagtCCTCCCTTTTCGATAGCGACAAAGAC‑GGCC‑C‑CCCC‑ATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCTGCGCCATAAACCTGTAGCACACCATTATTGAGCGTAGTCTGGTTTGCcgc                               >  1:2147369/1‑140 (MQ=255)

                                                                                                          agtagtCCTCCCTTTTCGATAGCGACAAAGAC‑GGCC‑C‑CCCC‑ATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCTGCGCCATAAACCTGTAGCACACCATTATTGAGCGTAGTCTGGTTTGCcgc                               >  1:166588/1‑140 (MQ=255)

                                                                                                          agtagtCCTCCCTTTTCGATAGCGACAAAGAC‑GGCC‑C‑CCCC‑ATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCTGCGCCATAAACCTGTAGCACACCATTATTGAGCGTAGTCTGGTTTGCcgc                               >  1:2622712/1‑140 (MQ=255)

                                                                                                          agtagtCCTCCCTTTTCGATAGCGACAAAGAC‑GGCC‑C‑CCCC‑ATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCTGCGCCATAAACCTGTAGCACACCATTATTGAGCGTAGTCTGGTTTGCcgc                               >  2:8507000/1‑140 (MQ=255)

                                                                                                              gtCCTCCCTTTTCGATAGCGACAAAGAC‑GGCC‑C‑CCCC‑ATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCTGCGCCATAAACCTGTAGCACACCATTATTGAGCGTAGTCTGGTTTGCCGCGCCa                           <  1:6017651/140‑1 (MQ=255)

                                                                                                              gtCCTCCCTTTTCGATAGCGACAAAGAC‑GGCC‑C‑CCCC‑ATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCTGCGCCATAAACCTGTAGCACACCATTATTGAGCGTAGTCTGGTTTGCCGCGCCa                           <  1:700664/140‑1 (MQ=255)

                                                                                                              gtCCTCACTTTTCGATCGCGACAAAGAC‑GGCC‑C‑CCCC‑CTCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCAGTCGCTGCGCCATAAACCTGTAGCACACCATTATTGAGCGTAGTCTGGTTTGACGCGCCa                           <  2:6211156/140‑1 (MQ=255)

                                                                                                              gtCCACCCATTTCGATACCGACAAAGAC‑GGCC‑C‑CCCC‑CACTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCTGCGCCATAAACCTGTAGCACACCATTATTGAGCGTAGTCTGGTTTGCCGCGCCa                           <  1:9119386/140‑1 (MQ=255)

                                                                                                                cctccCTTTTCGATAGCGACAAAGAC‑GGCC‑C‑CCCC‑ATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCTGCGCCATAAACCTGTAGCACACCATTATTGAGCGTAGTCTGGTTTGCCGCGCCAcc                         >  2:188077/1‑140 (MQ=255)

                                                                                                                 ctccCTTTTCGATAGCGACAAAGAC‑GGCC‑C‑CCCC‑ATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCTGCGCCATAAACCTGTAGCACACCATTATTGAGCGTAGTCTGGTTTGCCGCGCCAccc                        >  1:9221612/1‑140 (MQ=255)

                                                                                                                 ctccCTTTTCGATAGCGACAAAGAC‑GGCC‑C‑CCCC‑ATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCTGCGCCATAAACCTGTAGCACACCATTATTGAGCGTAGTCTGGTTTGCCGCGCCAccc                        >  1:3166741/1‑140 (MQ=255)

                                                                                                                 ctccCTTTTCGATAGCGACAAAGAC‑GGCC‑C‑CCCC‑ATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCTGCGCCATAAACCTGTAGCACACCAGTATTGAGCGTTGTCTGGTTTGCCGCGCCAccc                        >  1:8031361/1‑140 (MQ=255)

                                                                                                                   cccTTTTCGATAGCGACAAAGAC‑GGCC‑C‑CCCC‑ATCTTTTTCAACGAATAAGCGCCCGCCTTTAATCGTGGTATCTTTCGCTGCGCCATAAACCTGTAGCACCCCATTATTGAGCGTAGTCTGGTTTGCCGCGCCACCCAt                      >  2:4689676/1‑140 (MQ=255)

                                                                                                                      ttttCGATAGCGACAAAGAC‑GGCC‑C‑CCCC‑ATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCTGCGCCATAAACCTGTAGCACACCATTATTGAGCGTAGTCTGGTTTGCCGCGCCACCCATTTg                   >  2:2976348/1‑140 (MQ=255)

                                                                                                                      ttttCGATAGCGACAAAGAC‑GGCC‑C‑CCCC‑ATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCTGCGCCATAAACCTGTAGCACACCATTATTGAGCGTAGTCTGGTTTGCCGCGCCACCCATTTg                   <  2:2520547/140‑1 (MQ=255)

                                                                                                                         tCGATAGCGACAAAGAC‑GGCC‑C‑CCCC‑ATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCTGCGCCATAAACCTGTAGCACACCATTATTGAGCGTAGTCTGGTTTGCCGCGCCACCCATTTGAAc                <  1:4988890/140‑1 (MQ=255)

                                                                                                                              aGCGACAAAGAC‑GGCC‑C‑CCCC‑ATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCTGCGCCATAAACCTGTAGCACACCAGTATTGAGCGTAGTCTGGTTTGCCGCGCCACCCATTTGAACAAttt           >  1:4113734/1‑140 (MQ=255)

                                                                                                                                 gACAAAGAC‑GGCC‑C‑CCCC‑ATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCTGCGCCATAAACCTGTAGCACACCATTATTGAGCGTAGTCTGGTTTGCCGCGCCACCCATTTGAACAATTTGAt        <  1:4207877/140‑1 (MQ=255)

                                                                                                                                  aCAAAGAC‑GGCC‑C‑CCCC‑ATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCTGCGCCATAAACCTGTAGCACACCATTATTGAGCGTAGTCTGGTTTGCCGCGCCACCCATTTGAACAATTTGATa       >  2:5249724/1‑140 (MQ=255)

                                                                                                                                  aCAAAGAC‑GGCC‑C‑CCCC‑ATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCTGCGCCATAAACCTGTAGCACACCATTATTGAGCGTAGTCTGGTTTGCCGCGCCACCCATTTGAACAATTTGATa       >  2:4332124/1‑140 (MQ=255)

                                                                                                                                      aGAC‑GGCC‑C‑CCCC‑ATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCTGCGCCATAAACCTGTAGCACACCATTATTGAGCGTAGTCTGGTTTGCCGCGCCACCCATTTGAACAATTTGATAACCg   <  1:1122023/140‑1 (MQ=255)

                                                                                                                                      aGAC‑GGCC‑C‑CCCC‑ATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCTGCGCCATAAACCTGTAGCACACCATTATTGAGCGTAGTCTGGTTTGCCGCGCCACCCATTTGAACAATTTGATAACCg   <  2:1372015/140‑1 (MQ=255)

                                                                                                                                      aGAC‑GGCC‑C‑CCCC‑ATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCTGCGCCATAAACCTGTAGCACACCATTATTGAGCGTAGTCTGGTTTGCCGCGCCACCCATTTGAACAATTTGATAACCg   <  1:6570875/140‑1 (MQ=255)

                                                                                                                                      aGAC‑GGCC‑C‑CCCC‑ATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCTGCGCCATAAACCTGTAGCACACCATTATTGAGCGTAGTCTGGTTTGCCGCGCCACCCATTTGAACAATTTGATAACCg   <  2:3974732/140‑1 (MQ=255)

                                                                                                                                      aGAC‑GGCC‑C‑CCCC‑ATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCTGCGCCATAAACCTGTAGCACACCATTATTGAGCGTAGTCTGGTTTGCCGCGCCACCCATTTGAACAATTTGATAACCg   <  1:4013541/140‑1 (MQ=255)

                                                                                                                                      aGAC‑GGCC‑C‑CCCC‑ATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCAGTCGCTGCGCCATAAACCTGTAGCACACCATTATTGAGCGTAGTCTGGTTTGCCGCGCCACCCATTTGAACAATTTGATAACCg   <  2:7763710/140‑1 (MQ=255)

                                                                                                                                      aGAC‑GGCC‑C‑CCCC‑AGCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCTGCGCCATAAACCTGTAGCACACCATGATTGAGCGTAGTCTGGGTTGCCGCGCCACCCATTTGAACAATTTGATACCCg   <  2:2372971/140‑1 (MQ=255)

ACCGAGACGGTTTGTTCCGAATACCTCCATGGCCCGCGTGGTTGTTTTAATAGCACCGCCTGCTTTCTGATCTACCGCAAATGCAAATCCCCCCTCTTTAACCTCCAGTAGTCCTCCCTTTTCGATAGCGACAAAGAC‑GG‑C‑C‑CCCC‑ATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCTGCGCCATAAACCTGTAGCACACCATTATTGAGCGTAGTCTGGTTTGCCGCGCCACCCATTTGAACAATTTGATAACCGT  >  NZ_CP025268/2292451‑2292724

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`