BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP025268	2,466,923	(C)_8→9	intergenic (+242/‑123)	fadL → / → CXP41_RS12955	long‑chain fatty acid transporter FadL/protein YfdF

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP025268	2,466,915	1	.	C	93.1%	818.5 / 58.7	274	intergenic (+234/‑131)	fadL/CXP41_RS12955	long‑chain fatty acid transporter FadL/protein YfdF
Reads supporting (aligned to +/- strand): ref base . (1/18); new base C (43/212); total (44/230)
Fisher's exact test for biased strand distribution p-value = 3.28e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 345 total aligned reads displayed.

TTTGCAAACAGCCTGTTGGCAGCTTGCCCTTTCAAATCAATAAGCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATAC‑T‑‑C‑C‑CC‑C‑CC‑‑CTGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGATAGTCTATTTCATTAGGTAATATATATTTGTAACAAATCAATCAAAATGGAATAAAATCATG  >  NZ_CP025268/2466777‑2467048

  tGCAAACAGCCTGTTGGCAGCTTGCCCTTTCAAATCAATACGCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATAC‑T‑‑c‑c‑c                                                                                                                                        <  1:602554/140‑1 (MQ=255)

  tGCAAACAGCCTGTTGGCAGCTTGCCCTTTCAAATCAATAAGCGGTATCCACAGACAAACCTGAAGGGAAATGCATATTTTCAGGCGTACTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATAC‑T‑‑c‑c‑c                                                                                                                                        <  2:218548/140‑1 (MQ=255)

  tGCAAACAGCCTGTTGGCAGCTTGCCCTTTCAAATCAATAAGCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATAC‑T‑‑c‑c‑c                                                                                                                                        <  1:7452630/140‑1 (MQ=255)

  tGCAAACAGCCTGTTGGCAGCTTGCCCTTTCAAATCAATAAGCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATAC‑T‑‑c‑c‑c                                                                                                                                        <  1:3886389/140‑1 (MQ=255)

  agCACACAGCCTGTTGGCAGCTTGCCCTTTCAAATCAATAACCGGTAACCACAGCCAAACCTGAAGGGAAAGGCATATGTTCAGGCGTTCTGCTCGTCCTACACAAAGAGTTTACTTTTCTGCATTTCCAGGATAC‑T‑‑c‑c‑c                                                                                                                                        <  1:5410712/139‑1 (MQ=255)

    cAAACAGCCTGTTGGCAGCTTGCCCTTTCAAATCAATAAGCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATAC‑T‑‑c‑c‑cc‑c                                                                                                                                     <  1:1293403/140‑1 (MQ=255)

    cAAACAGCCTGTTGGCAGCTTGCCCTTTCAAATCAATAAGCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATAC‑T‑‑c‑c‑cc‑c                                                                                                                                     <  1:8892317/140‑1 (MQ=255)

      caCAGCCTGTTGGCAGCTTGCCCTTTCAAATCAATAAGCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATAC‑T‑‑c‑c‑cc‑c‑cc                                                                                                                                  <  1:5849906/139‑1 (MQ=255)

      aaCAGCCTGTTGGCAGCTTGCCCTTTCAAATCAATAAGCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCGGGATAC‑T‑‑c‑c‑cc‑c‑cc                                                                                                                                  <  2:1416055/140‑1 (MQ=255)

      aaCAGCCTGTTGGCAGCTTGCCCTTTCAAATCAATAAGCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATAC‑T‑‑c‑c‑cc‑c‑cc                                                                                                                                  <  2:5134540/140‑1 (MQ=255)

      aaCAGCCTGTTGGCAGCTTGCCCTTTCAAATCAATAAGCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATAC‑T‑‑c‑c‑cc‑c‑cc                                                                                                                                  <  1:840125/140‑1 (MQ=255)

      aaCAGCCTGTTGGCAGCTTGCCCTTTCAAATCAATAAGCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATAC‑T‑‑c‑c‑cc‑c‑cc                                                                                                                                  <  1:4620742/140‑1 (MQ=255)

      aaCAGACTGTTGGCAGCTTGCCCTTTCAAATCAATAAGCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATAC‑T‑‑c‑c‑cc‑c‑cc                                                                                                                                  <  2:8390448/140‑1 (MQ=255)

        ccgCCTGTTGGCAGCTTGCCCTTTCAAATCAATAAGCGGTATCCACAGACAAACCTGCAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATAC‑T‑‑c‑c‑cc‑c‑cc‑‑cc                                                                                                                              <  2:9287520/138‑2 (MQ=255)

        cAGCCTGTTGGCAGCTTGCCCTTTCACATCAATCAGCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATAC‑T‑‑c‑c‑cc‑c‑cc‑‑cc                                                                                                                              <  1:103377/140‑2 (MQ=255)

        cAGCCTGTTGGCAGCTTGCCCTTTCAACTCAATCCGCGGTATCCCCAGACAACCCTGAAGGGCAAGGCATATTTTCAGGCGGTCTGCTCGTACTTCTCAAATATTTTACTTTTCTGCATTGCCAGGATAC‑T‑‑c‑c‑cc‑c‑cc‑‑cc                                                                                                                              <  2:1051415/140‑2 (MQ=255)

        cAGCCTGTTGGCAGCTTGCCCTTTCAAATCAATAAGCGGTATCCACAGACAAACCTGAAGGGAAATGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATAC‑T‑‑c‑c‑cc‑c‑cc‑‑cc                                                                                                                              <  2:1763390/140‑2 (MQ=255)

        cAGCCTGTTGGCAGCTTGCCCTTTCAAATCAATAAGCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATAC‑T‑‑c‑c‑cc‑c‑cc‑‑cc                                                                                                                              <  2:7230201/140‑2 (MQ=255)

        cAGCCTGTAGGCAGCTTGCCCTTTCAAATCAATAAGCGGTATCCACAGACAAACCTGCAGTGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATAC‑T‑‑c‑c‑cc‑c‑cc‑‑cc                                                                                                                              <  2:7831212/140‑2 (MQ=255)

         aGCCTGTTGGCAGCTTGCCCTTTCAAATCAATAAGCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATAC‑T‑‑c‑c‑cc‑c‑cc‑‑ccg                                                                                                                             >  1:6866050/1‑138 (MQ=255)

           ccTGTTGGCAGCTTGCCCTTTCAAATCAATACGCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATAC‑T‑‑c‑c‑cc‑c‑cc‑‑cctgg                                                                                                                           <  1:4377624/140‑5 (MQ=255)

           ccTGTTGGCAGCTTGCCCTTTCAAATCAATAAGCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGTATAC‑T‑‑c‑c‑cc‑c‑cc‑‑cctgg                                                                                                                           <  2:4100931/140‑5 (MQ=255)

               ttGGCAGCTTGCCCTTTCAAATCAATAAGCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATAC‑TC‑C‑C‑CC‑C‑CC‑‑CTGGCTAt                                                                                                                        <  2:7964550/140‑1 (MQ=255)

                 ggCAGCTTGCCCTTTCAAATCAATAAGCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATAC‑TC‑C‑C‑CC‑C‑CC‑‑CTGGCTATtg                                                                                                                      <  1:2989099/140‑1 (MQ=255)

                   catcTTGACCTTTCAAATCAATAATCGTAATCAACAGACAAACCAGAAGGGAAAGGCATATTTTCACGCGTTCTGCTCGTCCTTATCAAAGAGTTTACTTTTATGCATTTCCAGGATAC‑TC‑C‑C‑CC‑C‑CC‑‑CTGGCTATtgtg                                                                                                                    <  2:9110237/137‑1 (MQ=255)

                   cAGCTTGCCCTTTCAAATCAATAAGCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATAC‑TC‑C‑C‑CC‑C‑CC‑‑CTGGCTATtgtg                                                                                                                    <  1:6520804/140‑1 (MQ=255)

                   cAGCTTGCCCTTTCAAATCAATAAGCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATAC‑TC‑C‑C‑CC‑C‑CC‑‑CTGGCTATtgtg                                                                                                                    <  1:3307806/140‑1 (MQ=255)

                   cAGCTTGCCCTTTCAAATCAATAAGCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATAC‑TC‑C‑C‑CC‑C‑CC‑‑CTGGCTATtgtg                                                                                                                    <  1:5884782/140‑1 (MQ=255)

                   cAGCTTGCCCTTTCAAATCAATAAGCGGTATCCAAAGCCAAAACTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTATCAAAGAGTTTACTTTTCTGCATTTCCAGGATAC‑T‑‑CAC‑AA‑C‑CC‑‑CTTGCTATtgtt                                                                                                                    <  2:3336702/140‑2 (MQ=255)

                     gCTTGCCCTTTCAAATCAATAAGCGGTATCCACATACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATAC‑TC‑C‑C‑CC‑C‑CC‑‑CTGGCTATTGTgcg                                                                                                                  <  2:4338634/140‑1 (MQ=255)

                     gCTTGCCCTTTCAAATCAATAAGCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATAC‑TC‑C‑C‑CC‑C‑CC‑‑CTGGCTATTGTgcg                                                                                                                  <  2:5426672/140‑1 (MQ=255)

                              ttCAAATCAATAAGCGGTATCCACAGNCAAACCTGCAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATAC‑TC‑C‑C‑CC‑C‑CC‑‑CTGGCTATTNTGCGCTCATacac                                                                                                         <  1:5540786/140‑1 (MQ=255)

                               tCAAATCAATACGCGGAATCCACAGACAACCCTCAAGGGCAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATAC‑T‑‑CAC‑CC‑C‑CC‑‑CTGGCTATTGTGCGCTCATACACt                                                                                                        <  2:3171828/140‑1 (MQ=255)

                               tCAAATCAATAAGCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATAC‑TC‑C‑C‑CC‑C‑CC‑‑CTGGCTATTGTGCGCTCATACACt                                                                                                        <  1:1776347/140‑1 (MQ=255)

                               tCAAATCAATAAGCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATAC‑TC‑C‑C‑CC‑C‑CC‑‑CTGGCTATTGTGCGCTCATACACt                                                                                                        <  1:7214539/140‑1 (MQ=255)

                               acacaTCAATAAGCGGCATCCACACCCAAACCTGAAGGGAAAGGCTTATTTTCAGCCGTACTGCACTTACGTCTCAAAGAGTTTACTTGTCTGCAAGTCCATGCTAC‑T‑‑C‑CACC‑C‑CC‑‑GTGGCTATTGTGCGCTCATACACt                                                                                                        <  2:7316936/136‑1 (MQ=255)

                                cAAATCAATCAGCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATAC‑TC‑C‑C‑CC‑C‑CC‑‑CTGGCTATTGTGCGCTCATACACTc                                                                                                       <  2:4800814/140‑1 (MQ=255)

                                cAAATCAATAAGCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATAC‑TC‑C‑C‑CC‑C‑CC‑‑CTGGCTATTGTGCGCTCATACACTc                                                                                                       <  2:4155677/140‑1 (MQ=255)

                                cAAATCAATAAGCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATAC‑TC‑C‑C‑CC‑C‑CC‑‑CTGGCTATTGTGCGCTCATACACTc                                                                                                       <  1:7972205/140‑1 (MQ=255)

                                cAAATCAATAAGCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATAC‑TC‑C‑C‑CC‑C‑CC‑‑CTGGCTATTGTGCGCTCATACACTc                                                                                                       <  2:2765615/140‑1 (MQ=255)

                                cAAATCAAGAAGCGGTATCCACAGACAACCCTGAAGGGAAAGGCATATTTTGAGGCGTTCTGCTCGTCCTTCTAAAAGCGGTTACTTTTCTGCATTTCCAGGATAC‑TC‑C‑C‑CC‑C‑CC‑‑CTGGCTATTGTGCGCTCATACACTc                                                                                                       <  2:6022019/140‑1 (MQ=255)

                                     cAATAAGCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGATTTTACTTTTCTGCATTTCCAGGATAC‑TC‑C‑C‑CC‑C‑CC‑‑CTGGCTAATGTGCGCTCATACACTCAAAtt                                                                                                  >  1:2822932/1‑140 (MQ=255)

                                           gCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCTTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATAC‑TC‑C‑C‑CC‑C‑CC‑‑CTGGCTATTGTGCGCTCATACACTCAAATTAAAGAt                                                                                            <  1:2368635/140‑1 (MQ=255)

                                           gCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGTGTTTACTTTTCTGCATTTCCAGGATAC‑TC‑C‑C‑CC‑C‑CC‑‑CTGGCTATTGTGCGCTCATACACTCAAATTAAAGAt                                                                                            <  1:738834/140‑1 (MQ=255)

                                           gCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATAC‑TC‑C‑C‑CC‑C‑CC‑‑CTGGCTATTGTGCGCTCATACACTCAAATTAAAGAt                                                                                            <  2:4571442/140‑1 (MQ=255)

                                           gCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATAC‑TC‑C‑C‑CC‑C‑CC‑‑CTGGCTATTGTGCGCTCATACACTCAAATTAAAGAt                                                                                            <  1:9524807/140‑1 (MQ=255)

                                           gCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATAC‑TC‑C‑C‑CC‑C‑CC‑‑CTGGCTATTGTGCGCTCATACACTCAAATTAAAGAt                                                                                            <  1:4484908/140‑1 (MQ=255)

                                           gCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATAC‑TC‑C‑C‑CC‑C‑CC‑‑CTGGCTATTGTGCGCTCATACACTCAAATTAAAGAt                                                                                            <  2:5296721/140‑1 (MQ=255)

                                           gCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATAC‑TC‑C‑C‑CC‑C‑CC‑‑CTGGCTATTGTGCGCTCATACACTCAAATTAAAGAt                                                                                            <  1:2657022/140‑1 (MQ=255)

                                           gCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATAC‑TC‑C‑C‑CC‑C‑CC‑‑CTGGCTATTGTGCGCTCATACACTCAAATTAAAGAt                                                                                            <  2:8560345/140‑1 (MQ=255)

                                           gCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATAC‑TC‑C‑C‑CC‑C‑CC‑‑CTGGCTATTGTGCGCTCATACACTCAAATTAAAGAt                                                                                            <  1:3470105/140‑1 (MQ=255)

                                           gCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTACTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATAC‑T‑‑C‑C‑CCAC‑CC‑‑CTGGCTATTGTGCGCTCATACACTCAAATTAAAGAt                                                                                            <  2:2078743/140‑1 (MQ=255)

                                           gCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTGTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTCCATTTCCAGGATAC‑TC‑C‑C‑CC‑C‑CC‑‑CTGGCTATTGTGCGCTCAGACACTCAAATTAAAGAt                                                                                            <  1:2082413/140‑1 (MQ=255)

                                           gCGGTATCCACAGACAAACCAGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATAC‑TC‑C‑C‑CC‑C‑CC‑‑CTGGCTATTGTGCGCTCATACACTCAAATTAAAGAt                                                                                            <  1:5673195/140‑1 (MQ=255)

                                              ggaTCCACAGACAAACCTGAAGGGAAACGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATAC‑TC‑C‑C‑CC‑C‑CC‑‑CAGGCTATTGTGCGCTCATACACTCAAATTAAAGATAgg                                                                                         <  1:9463006/138‑1 (MQ=255)

                                               tATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATAC‑TC‑C‑C‑CC‑C‑CC‑‑CTGGCTATTGTGCGCTCATACACTCAAATTAAAGAt                                                                                            <  2:4306195/136‑1 (MQ=255)

                                                aTCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATAC‑TC‑C‑C‑CC‑C‑CC‑‑CTGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGtt                                                                                       <  1:6403908/140‑1 (MQ=255)

                                                aTCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATAC‑TC‑C‑C‑CC‑C‑CC‑‑CTGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGtt                                                                                       <  2:4713840/140‑1 (MQ=255)

                                                aTCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATAC‑TC‑C‑C‑CC‑C‑CC‑‑CTGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGtt                                                                                       <  2:3961459/140‑1 (MQ=255)

                                                aTCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATAC‑TC‑C‑C‑CC‑C‑CC‑‑CTGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGtt                                                                                       <  1:6667521/140‑1 (MQ=255)

                                                 tCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATAC‑T‑‑c‑c‑cc‑c‑cc                                                                                                                                  >  2:3074687/1‑97 (MQ=255)

                                                 tCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATAC‑T‑‑C‑C‑CC‑C‑CC‑‑CTGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCt                                                                                     >  2:1998730/1‑140 (MQ=255)

                                                      aGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATAC‑TC‑C‑C‑CC‑C‑CC‑‑CTGGGTATTGTGCGCTCATACACTCAATTTAAAGATAGGTTCtaaat                                                                                 >  1:378269/1‑140 (MQ=255)

                                                      aGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATAC‑TC‑C‑C‑CC‑C‑CC‑‑CTGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCtaaat                                                                                 >  1:3636088/1‑140 (MQ=255)

                                                             ccTGTAGGGTTAGGCATATTTTCAGGCGTTCTGCTCGTACTTCAAAAAGAGTTTACTTTTCTGCATTTCCAGGTTAA‑T‑‑C‑CACC‑C‑CC‑‑CTGGCTATTGTGCGCTCATACACTCAAATTAAAGATTGTTTCTAAATAAATGAg                                                                          <  2:6906058/140‑1 (MQ=255)

                                                             ccTGAAGGGTAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGATTTTACTTTTCTGCATTTCCTGGATAC‑TC‑C‑C‑CC‑C‑CC‑‑CTGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAg                                                                          <  1:5061113/140‑1 (MQ=255)

                                                             ccTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATAC‑TC‑C‑C‑CC‑C‑CC‑‑CTGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAg                                                                          <  2:6460094/140‑1 (MQ=255)

                                                             ccTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATAC‑TC‑C‑C‑CC‑C‑CC‑‑CTGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAg                                                                          <  1:810660/140‑1 (MQ=255)

                                                             ccTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATAC‑TC‑C‑C‑CC‑C‑CC‑‑CTGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAg                                                                          <  2:1318186/140‑1 (MQ=255)

                                                              cTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATAC‑TCCC‑C‑CC‑C‑CC‑‑CTGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAg                                                                          <  1:3127951/140‑1 (MQ=255)

                                                                  aGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATAC‑TC‑C‑C‑CC‑C‑CC‑‑CTGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGATCGttt                                                                     >  1:9297974/1‑140 (MQ=255)

                                                                       aaGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCCGGATAC‑TC‑C‑C‑CC‑C‑CC‑‑CTGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGa                                                                <  1:7322333/140‑1 (MQ=255)

                                                                       aaGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCCGGATAC‑TC‑C‑C‑CC‑C‑CC‑‑CTGGCTATTGTGCGCGCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGa                                                                <  2:9330456/140‑1 (MQ=255)

                                                                       aaGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATAC‑TC‑C‑C‑CC‑C‑CC‑‑CTGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGa                                                                <  2:1169439/140‑1 (MQ=255)

                                                                       aaGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATAC‑TC‑C‑C‑CC‑C‑CC‑‑CTGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGa                                                                <  2:1524614/140‑1 (MQ=255)

                                                                       aaGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATAC‑TC‑C‑C‑CC‑C‑CC‑‑CTGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGa                                                                <  1:7136562/140‑1 (MQ=255)

                                                                           cATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATAC‑TC‑C‑C‑CC‑C‑CC‑‑CTGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGATAGt                                                            <  2:882993/140‑1 (MQ=255)

                                                                              atTTTCAGGCGTTCTGCTCTTCCTTCTCAATTAGTTTAATTTTATGCATTTCAAGTATAC‑TC‑C‑C‑CC‑C‑CC‑‑CTGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGATAGTCTa                                                         <  2:3560185/140‑1 (MQ=255)

                                                                              atTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATAC‑TC‑C‑C‑CC‑C‑CC‑‑CTGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGTTAGTCTa                                                         >  1:2909204/1‑140 (MQ=255)

                                                                              atTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATAC‑TC‑C‑C‑CC‑C‑CC‑‑CTGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGATAGTCTa                                                         <  1:3081045/140‑1 (MQ=255)

                                                                                   cagtagTTCTGCTAGTCATTCTCAAATATTTTACTTTTGTTAATTTAGAGTTTAC‑T‑‑C‑C‑CC‑CACC‑‑CTCGCTATTGTGCGCTCATTCACTCTAATTAAATATAGGTTCTAATTAAATGTGCGATTTTTGATAGTCTATTTCa                                                    <  2:5822436/135‑1 (MQ=255)

                                                                                   cAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTCCTTTTCTGCATTTCCATGATAC‑TC‑C‑C‑CC‑C‑CC‑‑CTGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGATAGTCTATTTCa                                                    <  2:7677992/140‑1 (MQ=255)

                                                                                   cAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATAC‑TC‑C‑C‑CC‑C‑CC‑‑CTGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGATAGTCTATTTCa                                                    <  1:4078612/140‑1 (MQ=255)

                                                                                   cAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATAC‑TC‑C‑C‑CC‑C‑CC‑‑CTGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGATAGTCTATTTCa                                                    <  1:5223047/140‑1 (MQ=255)

                                                                                   cAGGCGTTCTGCTCGTCCCACTCAAAGAGTTTACTTTTCTGCATTTCCAGGATAC‑TC‑C‑C‑CC‑C‑CC‑‑CTGGCTATTGTGCGCTCATACACTCAAATTACAGATAGGTTCTAAATAAATGAGCGTTTTTTGATAGTCTATTTCa                                                    <  1:3671253/140‑1 (MQ=255)

                                                                                     ggCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATAC‑TC‑C‑C‑CC‑C‑CC‑‑CTGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGATAGTCTATTTCAtt                                                  <  2:5117701/140‑1 (MQ=255)

                                                                                     ggCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATAC‑TC‑C‑C‑CC‑C‑CC‑‑CTGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGATAGTCTATTTCAtt                                                  <  2:5645149/140‑1 (MQ=255)

                                                                                     ggCGTTCTGCTCGTCCTTCTAAAAGAGTTTACTTTTCTGCATTTCCAGGATAC‑TC‑C‑C‑CC‑C‑CC‑‑CTGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGATAGTCTATTTCAtt                                                  <  2:2224300/140‑1 (MQ=255)

                                                                                      gCGTTCTGCTCGTCCTTCTCACAGAGTTTACATATCTGCATATCCACGATACGC‑‑C‑C‑CC‑C‑CC‑‑CTCGCTATTGTGCGCTCCTACACTCAAATTAAAGATAGGTACTAACAAAATGAGCGTTTTTTGATAGACTATTTCTTTa                                                 <  2:8210938/140‑1 (MQ=255)

                                                                                         ttCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATAC‑TC‑C‑C‑CC‑C‑CC‑‑CTGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGATAGTCTATTTCATTAGGt                                              >  2:7520607/1‑140 (MQ=255)

                                                                                           cTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATAC‑TC‑C‑C‑CC‑C‑CC‑‑CTGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGATAGTCTATTTCATTAGGTaa                                            >  2:9396550/1‑140 (MQ=255)

                                                                                              cTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATAC‑TC‑C‑C‑CC‑C‑CC‑‑CTGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGATAGTCTATTTCATTAGGTAatat                                         <  2:679837/140‑1 (MQ=255)

                                                                                                   ccTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATAC‑TC‑C‑C‑CC‑C‑CC‑‑CTGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGATAGTATATTTCATTAGGTACTATATAtt                                    >  1:8754154/1‑140 (MQ=255)

                                                                                                      tctcAAAGAGTTTACTTTTCTGCATTTCCAGGATAC‑TC‑C‑C‑CC‑C‑CC‑‑CTGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGATAGTCTATTTCATTAGGTAATATATATTTGt                                 <  1:2579800/140‑1 (MQ=255)

                                                                                                        tcAAAGAGTTTACTTTTCTGCATTTCCAGGATAC‑TC‑C‑C‑CC‑C‑CC‑‑CTGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGATAGTCTATTTCATTAGGTAATATATATTTGtta                               >  1:7723758/1‑138 (MQ=255)

                                                                                                                      tttCTGCAATTCCAGGATAC‑T‑‑C‑C‑CC‑CNCC‑‑CTGGCTATGGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAAGAACTGAGCGTTTTTTGATAGTCTATTTCATTAGGTAATATATAATTGTAACAAATCAATCaaaa                 <  1:6350879/140‑1 (MQ=255)

                                                                                                                          tGCATTTCCAGGATAC‑TC‑C‑C‑CC‑C‑CC‑‑CTGGCTATTGTGCGCTCATACACTCAAATTAAAGAt                                                                                            >  2:3743500/1‑61 (MQ=255)

                                                                                                                                 ccAGGATAC‑T‑‑C‑C‑CC‑C‑CCCACTGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGATAGTCTATTTCATTAGGTAATATATATTTGTAACAAATCAATCAAAATGGAATaaaa       <  2:1889170/140‑1 (MQ=255)

                                                                                                                                   aGGATAC‑TC‑C‑C‑CC‑C‑CC‑‑CTGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGATAGTCTATTTCATTAGGTAATATATATTTGTAACAAATCAATCAAAATGGAATAAAATCa    <  2:6094668/140‑1 (MQ=255)

                                                                                                                                     gATAC‑TC‑C‑C‑CC‑C‑CC‑‑CTGGCTATTTTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGATAGTCTATTTCATTAGGTAATATATTTTTGTAACAAATCAATCAAAATGGAATAAAATCATg  <  2:9536822/140‑1 (MQ=255)

TTTGCAAACAGCCTGTTGGCAGCTTGCCCTTTCAAATCAATAAGCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATAC‑T‑‑C‑C‑CC‑C‑CC‑‑CTGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGATAGTCTATTTCATTAGGTAATATATATTTGTAACAAATCAATCAAAATGGAATAAAATCATG  >  NZ_CP025268/2466777‑2467048

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`