Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP025268164,3950AT21.9% 281.3 / 17.2 145intergenic (+30/‑166)hrpB/mrcBATP‑dependent helicase HrpB/bifunctional glycosyl transferase/transpeptidase
Reads supporting (aligned to +/- strand):  ref base A (55/52);  new base T (0/30);  total (56/89)
Fisher's exact test for biased strand distribution p-value = 1.87e-08
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

Only 100 of 159 total aligned reads displayed.

GAGCGTACCGTGAGGTGCAAAAAGAGATGAAAGGGCGTTATCCCAAACATG‑TCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCA‑CCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAG‑CACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATCGGGCTTTT  >  NZ_CP025268/164256‑164534
                                                                                                                                            |                                                                                                                                             
gAGCGTACCGTGAGGTGCAAAATGAGATGAAAGGGCGTTATCCCAAACATG‑TCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGgaga                                                                                                                                               >  1:360151/1‑140 (MQ=255)
gAGCGTACCGTGAGGTGCAAAAAGAGATGAAAGGGCGTTATCCCAAACATG‑TCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGgaga                                                                                                                                               >  1:6993165/1‑140 (MQ=255)
gAGCGTACCGTGAGGTGCAAAAAGAGATGAAAGGGCGTTATCCCAAACATG‑TCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGgaga                                                                                                                                               <  1:1696743/140‑1 (MQ=255)
gAGCGTACCGTGAGGTGCAAAAAGAGATGAAAGGGCGTTATCCCAAACATG‑TCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGgaga                                                                                                                                               >  2:6350316/1‑140 (MQ=255)
gAGCGTACCGTGAGGTGCAAAAAGAGATGAAAGGGCGTTATCCCAAACATG‑TCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCACTCTCCCTGTGGgaga                                                                                                                                               >  1:3794820/1‑140 (MQ=255)
 aGCGTACCGTGAGGTGCAAAAAGAGATGAAAGGGCGTTATCCCAAACATG‑TCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGagag                                                                                                                                              >  1:1571479/1‑140 (MQ=255)
   cGTACCGTGAGGTGCAAAAAGAGATGAAAGGGCGTTATCCCACACATG‑TCTGGCCGGACGACCCGGCAAATCCTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAggg                                                                                                                                            <  2:7467622/140‑1 (MQ=255)
   cGTACCGTGAGGTGCAAAAAGAGATGAAAGGGCGTTATCCCAAACATG‑TCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGACAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAggg                                                                                                                                            >  1:5944518/1‑140 (MQ=255)
   cGTACCGTGAGGTGCAAAAAGAGATGAAAGGGCGTTATCCCAAACATG‑TCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAACAAGTAGTCGTACGGGTGGTTTTCTCCCTCTCCCTGTGGGAGAggg                                                                                                                                            >  2:5819129/1‑140 (MQ=255)
   cGTACCGTGAGGTGCAAAAAGAGATGAAAGGGCGTTATCCCAAACATG‑TCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGTGAggg                                                                                                                                            >  1:6666832/1‑140 (MQ=255)
   cGTACCGTGAGGTGCAAAAAGAGATGAAAGGGCGTTATCCCAAACATG‑TCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAggg                                                                                                                                            <  2:198319/140‑1 (MQ=255)
   cGTACCGTGAGGTGCAAAAAGAGATGAAAGGGCGTTATCCCAAACATG‑TCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAggg                                                                                                                                            <  2:198259/140‑1 (MQ=255)
   cGTACCGTGAGGTGCAAAAAGAGATGAAAGGGCGTTATCCCAAACATG‑TCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAggg                                                                                                                                            >  1:411685/1‑140 (MQ=255)
   cGTACCGTGAGGTGCAAAAAGAGATGAAAGGGCGTTATCCCAAACATG‑TCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAggg                                                                                                                                            <  2:9106654/140‑1 (MQ=255)
    gTACCGTGAGTTGCAAAAAGACATGAAAGGGCGTTAACCCAAACATG‑TCTGGCCGGACGACCCGGAAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGc                                                                                                                                           <  1:1339928/140‑1 (MQ=255)
    gTACCGTGAGGTGCAAACAGAGATGAACGGGCGTTATCCCAAACATG‑TCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGc                                                                                                                                           <  1:9337299/140‑1 (MQ=255)
    gTACCGTGAGGTGCAAAAAGAGATGAAAGGGCGTTATCCCAAACATG‑TCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGc                                                                                                                                           <  2:1132618/140‑1 (MQ=255)
    gTACCGTGAGGTGCAAAAAGAGATGAAAGGGCGTTATCCCAAACATG‑TCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGc                                                                                                                                           <  2:4107113/140‑1 (MQ=255)
    gTACCGTGAGGTGCAAAAAGAGATGAAAGGGCGTTATCCCAAACATG‑TCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGc                                                                                                                                           <  1:5157382/140‑1 (MQ=255)
     tACCGTGAGGTGCAAAAAGAGATGAAAGGGCGTTATCCCAAACATG‑TCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGcc                                                                                                                                          >  2:4341033/1‑140 (MQ=255)
     tACCGTGAGGGGCAAAAAGAGAGGATAGGGCGTTCTCCCAAACATG‑TGTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAGAAAGTATTCGTCAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGcc                                                                                                                                          >  2:3422858/1‑140 (MQ=255)
      aCCGTGAGGTGCAAAAAGCGCTGAAAGGGCGTTATCCCAAACATG‑TCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCg                                                                                                                                         <  2:218385/140‑1 (MQ=255)
        cGTGAGGTGCAAAAAGAGATGAAAGGGCGTAATCCCAAACATG‑TCTGGCCGGACGACCCGGCAAATCCTGCACCGCCGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCCCCCTGTGGGAGAGGGCCggg                                                                                                                                       <  1:7879296/140‑1 (MQ=255)
        cGTGAGGTGAAAAAAGAGATGAAAGGGCGTTATCCCAAACATG‑TCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAATTTTTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCggg                                                                                                                                       <  2:1098566/140‑1 (MQ=255)
          tGAGGTGCAAAAAGAGATGAAAGGGCGTTATCCCAAACATG‑TCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGt                                                                                                                                     >  2:1606299/1‑140 (MQ=255)
             ggTGCAAAAAGAGATGAAAGGGCGTTATCCCAAACATG‑TCTGGCCGGCCGACCCGGCACATACTGCACCGACGCGACGGACGAAATAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTgtg                                                                                                                                  >  1:7475695/1‑138 (MQ=255)
               tGCAAAAAGAGATGAAAGGGCGTTATCCCAAACATG‑TCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAggg                                                                                                                                >  1:3308537/1‑140 (MQ=255)
               tGCAAAAAGAGATGAAAGGGCGTTATCCCAAACATG‑TCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAggg                                                                                                                                >  1:9535989/1‑140 (MQ=255)
                 cAAAAAGAGATGAAAGGGCGTTATCCCAAACATG‑TCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCa                                                                                                                              >  2:3644641/1‑140 (MQ=255)
                  aaaaaGAGATGAAAGGGCGTTATCCCAAACATG‑TCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCAt                                                                                                                             >  2:6778418/1‑140 (MQ=255)
                  aaaaaGAGATGAAAGGGCGTTATCCCAAACATG‑TCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCAt                                                                                                                             >  1:4775702/1‑140 (MQ=255)
                         gaTGAAAGGGCGTTATCCCAAACATG‑TCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTATGGTTGGTTTTCTCCCTCTCCCTGGGGGAGAGGGCCGGGGTGAGGGCATCAgcgcg                                                                                                                      <  2:9436684/140‑1 (MQ=255)
                         gaTGAAAGGGCGTTATCCCAAACATG‑TCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCCTCAgcgcg                                                                                                                      >  1:2242652/1‑140 (MQ=255)
                         caTGACAGTGCGTTAACCCAAACATG‑TCTGGCCGGCCGACCCGGCAAATACTGCACCGACGCGACGGACGAAACTGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGATAGGGCCGGGGTGAGGGCATCAgcgcg                                                                                                                      <  2:7764391/139‑1 (MQ=37)
                           ttaatgggCTTTATCCCAAACTTG‑TCTGGCCGGACGACCCGCAAAAAACTGCACCGACGCTCCGGACTAAAAATTATTCTTATGGTTGTTTTACTCCCACCCCCTGTGGTTGAGGGCGGGGGTGAGGGCCTCAGCGCGCa                                                                                                                    <  1:9534743/135‑1 (MQ=255)
                                  gCGTTATCCCAAACATG‑TCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTgcac                                                                                                             >  2:8271946/1‑140 (MQ=255)
                                   cGTTATCCCAAACATG‑TCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGTACGAAAATGTTTTCTTATGGTTGGTTTTCTCCCTCTCCGTGTGGGTGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTtcacc                                                                                                            <  2:450103/140‑1 (MQ=38)
                                   cGTTATCCCAAACATG‑TCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCCTCAGCGCGCACGTtaacc                                                                                                            >  2:1619423/1‑140 (MQ=255)
                                    gTTATCCCAAACATG‑TCTGGCCGGACGACCCGGCAATTACTGCACCGACGCGACGGACTAAAATGTATTGTTATGGTTGTTTTTCTCCCTCTCCCTGTGGGAGTGGGCCGGGGTGTGGGCATCAGCGCGCACGTtcaccc                                                                                                           <  2:6761712/140‑1 (MQ=37)
                                    gTTATCCCAAACATG‑TCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTgcaccc                                                                                                           >  2:8882368/1‑140 (MQ=255)
                                     ttATCCCAAACATG‑ACGGCCCGGACGACCCGGCAATTACTCCACCTACGGGACGGAGGAAAAAGTATGCTTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGTGGGCGGGGTTGAGGGCCTCAGCGCGCACGTtcaccct                                                                                                          <  2:9523359/140‑1 (MQ=25)
                                     ttATCCCAAAAATG‑TCTGGCCGGACTACCCGGCAAATACGGCACCGACGCTCCGGACGAAAAAGTATTCTTATGGTTGGTTTTCTCCCTCTCCCTTTGGGTGAGGGCGGGGGTGAGGGCATCAGCGCGCACGTtcaccct                                                                                                          <  2:5125683/140‑1 (MQ=37)
                                      ttatccAAACATGTTCGGGCCGTACCACCCGGCAAATACTGAACCGACGCGAGGTACTAAAATGTATTCTTATGTTTGTTTTTCTCCCACTCCCTGGGGTTGAGGGCGGGGGTTAGGGCATCAGCGCGCACGTtcaccct                                                                                                          <  2:491066/136‑1 (MQ=255)
                                         cccAAACATG‑TCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACTGACTAAAAATTTTTCGTATGGTTGGTTTTCTCCCTCTCCGTGTGGGAGAGGGCCGGGGTGAGGGCCTCAGCGCGCACGTtcaccctca‑cc                                                                                                     <  2:7788594/140‑1 (MQ=38)
                                             caCATG‑GCGGGCCGGCCGCCCCGCCAAATACGCCCCCCACGCGCCGGACGAAAACGTTTTCGTAAGGTTGTTTTTCCCCCTCACCCTGGGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCA‑CCCTaa                                                                                                 <  1:7136215/139‑1 (MQ=21)
                                             aaCATG‑TCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCACA‑ACCTaa                                                                                                 >  2:4844058/1‑140 (MQ=255)
                                                ttG‑TCGGGCCGTACGACCCGGAAAATACTGCACCGACGCGACGGACGAAAAATTATTCTTATGGTTGGTTTAATCCCTCTCCCTGTGGTAGTGGGCCGGGTTTAGGGCATCAGCGCGCACGTTCACCCTCA‑CCCTAAccc                                                                                              <  2:325526/139‑1 (MQ=255)
                                                aTG‑TCTGGCCGGACCACCCGGCAAATACTGCCCCGACGCGCCGGACCAAACAGTATTCTAACGGTTGGTTTTCTCCCTCTCCCTGTGGGTGTGGGCCGGGGTGCGGGCCTCAGCGCGCACGTTCACCCTCA‑CCCTAAccc                                                                                              <  1:1755168/140‑1 (MQ=37)
                                                  g‑TCTGGCCTGACGCCCCGGAAAAACCTGCCCCGCCGCGACGGACGAAAAATTATTCTTAAGGTTGGTTTTCTCCCTCTCCATGTGGGAGCGGGCCGGGGTGAGGGCCTCAGCGCGCACGTTCACCCTCA‑CCCTAACCctc                                                                                            <  2:6328455/140‑1 (MQ=255)
                                                  g‑TCTGGCCGTACGCCCCGGCAATTAATGCACCGACGCGACGGACGAAAAAGTTTTCGTAAGGTTGTTTTTCTCCCTATCACTGGGGGTGTGGGCGGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCA‑CCCTAACCctc                                                                                            <  2:3141088/140‑1 (MQ=255)
                                                  g‑TCTGGCCGGCCGACCCGTAAAATACTGCACCGACGGGACGGACTAAAATTTATTCTTAAGGTTGTTTTTCCCCCTCGCCGTGTGTGTGTGGGCCGGGGTTTGGGCATCAGCGCGCACGTTCACCCTCA‑CCCTAACCctc                                                                                            <  1:4415548/140‑1 (MQ=255)
                                                  g‑TCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCCTCAGCGCGCACGTTCACCCTCA‑ACCTAAcccac                                                                                            >  2:8661109/1‑138 (MQ=255)
                                                  g‑TCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCCCGTTCACCCTCA‑CCCTAACCctc                                                                                            <  2:6823984/140‑1 (MQ=255)
                                                  g‑TCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCACA‑CCCTaaacaac                                                                                            >  1:8842247/1‑135 (MQ=255)
                                                  g‑TCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTGGACCCACACCCCTAAccac                                                                                             >  2:1896566/1‑138 (MQ=37)
                                                  g‑TCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTGCACCCACA‑CCCAAAcacac                                                                                            >  1:3282152/1‑136 (MQ=255)
                                                  g‑TCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCGGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCA‑CCCTAACCctc                                                                                            <  2:1502792/140‑1 (MQ=255)
                                                  g‑TCTGGCCGGACGACCCGGCAAAAACCGCACCGACGCGACGTACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCA‑CCCTAACCctc                                                                                            <  2:1297604/140‑1 (MQ=255)
                                                  g‑TCTGGCAGGACTACCGGGAAATTACTGAACCGACGCGAGGGACTAAAATGTTTTCGTAAGGTTGTTTTTCTCCCTCTCCCTTTGGGTGAGGGCCGGGGTTAGGGCATCATCGCGCACGTTCACCCTCA‑CCCTAACCctc                                                                                            <  2:6161372/140‑1 (MQ=255)
                                                  g‑TCGGGCCGGACGACCCGGCAAATACTGCCCCTCCGCGACGTACGAAAAAGTATTCGTATGGTTGGTTTTCTCCCACTCCCTGTGGGAGTGGGCGGGGGTGAGGCCCTCAGCGCGCACGTTCACCCTCA‑CCCTAACCctc                                                                                            <  2:8072903/140‑1 (MQ=255)
                                                  g‑TCGGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCA‑CCCTAACCctc                                                                                            <  2:7963158/140‑1 (MQ=255)
                                                   ctCTGGCCGGACGCCCCGGCAAATACTCCCCCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCGCCCACTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCA‑CCCTAACCctc                                                                                            <  2:6675294/139‑1 (MQ=38)
                                                     cTGGCCGGACTCCCCGGCAAACAAGGCCCCTACGCGACGGACCAAAATTTATTCGTACGGTTGGTTTCCACCCACCCCCTGTGGGTGAGGGCCGGGGTGTGGGCCTCAGCGCGCACGTTCACCCTCA‑CCCTAACCCtctc                                                                                          <  1:8884042/140‑1 (MQ=255)
                                                     cTGGCCGGACGCCCCGGCAAATACTGCACCTACGCGACGGACGAAAATGTATTCGTAAGGTTGGTTTTCTCCATCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCA‑CCCTAACCCtctc                                                                                          <  2:8692516/140‑1 (MQ=255)
                                                      tGGCCGGACGACCCGGCAAATACGGCACCGACGCGACGGACGAAAAATTATACGAAAGGTTGGTTTTCTCCCTCCCCCTGTGGGAGAGGGCCGGGGTGAGGGCCTCAGCGCGCACGTTCACCCTCA‑CCCTAACCCTCTcc                                                                                         <  2:5440367/140‑1 (MQ=38)
                                                      ggcccGTACGACCCGGCAAATACTCCACCGACCCGCCGGACTAAAAAGTTTTCGTAAGGTTGGTTTTCCCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCA‑CCCTAACCCTCTcc                                                                                         <  2:7654062/137‑1 (MQ=37)
                                                        gCCGGACGACCCGGCAAATACTGCCCCGACGCGACGGACGAAAATGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGTGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCA‑CCCTAACCCTCTCCCt                                                                                       <  2:9110734/140‑1 (MQ=255)
                                                        gCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCCCGTTCAACCTCA‑CCCAAACCCACACCCt                                                                                       >  2:3432910/1‑140 (MQ=38)
                                                            gacgacCCGGCAAATACTGCACCGCCGCGACGGACGAAAATGAATTCTTATGGTTGTTTTACTCCCTCTCCCTGTGGGAGAGGGCGGGGGTGAGGGCCTCAGCGCGCACGTTCACCCTCA‑CCCTAACCCTCTCCCTCAAg                                                                                   <  1:8006664/140‑1 (MQ=37)
                                                             acgacCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCCTCAGCGCGCACGGGCACCCACA‑CCCAAAACCACTCCCACAAgg                                                                                  >  2:3984711/1‑140 (MQ=37)
                                                                acCCGGCAAATACTGCACCGACGCGACGGACTAAAATGTATTCGTACGGTTGGTTTTCTCCCTCTCCCTGTGGTTGTGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCA‑CCCTAACCCTCTCCCTCAAGGgag                                                                               <  1:6778418/140‑1 (MQ=38)
                                                                acCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCCGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCA‑CCCTAACCCTCTCCCTCAAGGgag                                                                               <  2:6163290/140‑1 (MQ=255)
                                                                  ccGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTGGTCCCTAC‑CCCGAACCCGCAACCACCAGGgagtg                                                                             >  1:8629025/1‑138 (MQ=25)
                                                                     gCAAATACGGCACCGCCGCGCCGCACGAAACCGTATTGGTCCGGTTGGTTGACGCCCTCCCCGTGTGGGTGCGGGCGGGGGTGCGGGCCTCAGCGCGCACGTTCACCCTCA‑CCCTAACCCTCTCCCTCAAGGGAGAgggt                                                                          <  1:8658306/140‑2 (MQ=255)
                                                                       aaaTACTCCACCCACGCTACGTAATAAAAATTATTCTTTATTTTTTTTTTCCCCCACACCCTGTGGTAGAGGGCCGGGGTGAGGCCCTCAGCGCGCACGTTCACCCTCA‑CCCTAACCCTCTCCCTCAAGGGAGAGGGGAc                                                                        <  2:968640/140‑1 (MQ=255)
                                                                           aCTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGGTCACCCACA‑CCCAAAACCACAACCACAAAGGGGAGGGGGCCgct                                                                    >  2:1269538/1‑138 (MQ=25)
                                                                               cACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTGTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCA‑CCCTAACCctc                                                                                            >  1:8423967/1‑112 (MQ=255)
                                                                               cACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTGTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCA‑CCCTAACCctc                                                                                            <  2:8423967/112‑1 (MQ=255)
                                                                               cACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCCTCAGCGCGCACGGGGACCCGCA‑CCCCAACCCACTCCCGCAAGGGGGGGGGGGCCgcgcggg                                                                >  2:47856/1‑134 (MQ=25)
                                                                               cACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTGGGCCCACC‑CCCGAACCCGCTACCACCATGGGGGGGGGGCCGGACGAg                                                                >  1:9186307/1‑140 (MQ=18)
                                                                                 ccGACGCGACGGACTAAAAAGTATTCGTAAGTTTGGTTTTCTCCATCTCCCTGTGGGAGAGGGCCGGGGTTAGGGCATCAGCGCGCACGTTCACCCTCA‑CCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAG‑ca                                                             <  2:5736552/140‑1 (MQ=255)
                                                                                   gacgcgacgGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTGGCCCCGCA‑CCCTAAACCACAACCACAACGGGGGGGGGGCCCAAAGAG‑caca                                                           >  2:2087384/1‑140 (MQ=18)
                                                                                       cgacgGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGGTCCCCCACA‑CCCAAAACCTCTCCCTCAAGGGAGAGGGGACCGATCGAG‑CCCaaaat                                                       >  2:2606996/1‑138 (MQ=37)
                                                                                         acggacgAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCA‑CCCTAACCctc                                                                                            <  2:3656338/102‑1 (MQ=255)
                                                                                           gcaccaaaaaGTTTTCGTATGGTGGGTTTTCCCCCCCTCCGTGTGGGTGTGGCCGGGGGTGCGGGCATCAGCGCGCACGTTCACCCTCA‑CCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAG‑CACAAATTTTga                                                   <  1:9300413/135‑1 (MQ=37)
                                                                                            gacgAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCA‑CCCAAAACCACACCCACAAGGGAGAGGGGCCCGATCGAGCCCCAAAttttag                                                   >  2:7521523/1‑138 (MQ=25)
                                                                                                  aaaGTATTCGTATGTTTGGTTTTCTCCATCACCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCA‑CCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAG‑CACAAATTTTGAGAGatat                                            <  1:4427619/140‑1 (MQ=255)
                                                                                                   atgttttCGTTTGGTTGGTTTTCTCCCTCTCCCTGTGGGAGTGGGCGGGGGTGAGGGAATCAGCGCGCACGTTCACCCTCA‑CCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAG‑CACAAATTTTGAGAGATAtc                                           <  2:5522861/135‑1 (MQ=38)
                                                                                                   aaGTATTCGTATGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCA‑CCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAG‑CACAAATTTTGAGAGATAtc                                           <  2:541073/140‑1 (MQ=255)
                                                                                                       aTTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCAGCCGCGCGCACGTTCACCCAAA‑CCCAAACCCACACCCACAAGGGAGGGGGGACCGATAGAG‑CCCAAAATTTTAGAGTTAtattat                                       >  2:2999443/1‑138 (MQ=21)
                                                                                                        tacttatggttggttTTCGCCCTCTCCGTGTGGGTGTGGGCGGGGGTGCGGGCCTCAGCGCGCACGTTCACCCTCA‑CCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAG‑CACAAATTTTGAGAGATAtcttctt                                      <  1:4414417/133‑1 (MQ=38)
                                                                                                         tCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCCTCAGCGCGCACGTTCACCCTCA‑CCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAG‑CACAAATTTTGAGAGATAtcttcttc                                     <  2:2647235/140‑1 (MQ=255)
                                                                                                         tCGTAAGGTGGGTTCTCTCCCACACCCAGTGGGAGAGGGCCGGGGTGAGGGCAACAGCGCGCACGTTCACCCTCA‑CCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAG‑CACAAATTTTGAGAGATAtcttcttc                                     <  2:2032571/140‑1 (MQ=39)
                                                                                                             ctgttgtgttTCCCCCCCCGCCGTGTGGGTGTGGGCGGGGGTGCGGGCCTCAGCGCGCACGTTCACCCTCA‑CCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAG‑CACAAATTTTGAGAGATATCTTCTTCTGTc                                 <  1:2359967/133‑1 (MQ=37)
                                                                                                              tggttggttTCCTCCCTCGCCGTGGGGGTGGGGGCGGGGGTGCGGGCCTCAGCGCGCACGTTCACCCTCA‑CCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAG‑CACAAATTTTTAGAGATATCTTCTTCTGTCt                                <  1:207551/139‑1 (MQ=38)
                                                                                                                        tctccctctcccTTTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCA‑CCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAG‑CACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACagaa                      <  2:8222269/140‑1 (MQ=255)
                                                                                                                              gcgccctGTGGGAGTGGGCGGGGGTGAGGCCATCAGCGCGCACGTTCACCCTCA‑CCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAG‑CACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAg                <  1:116776/137‑1 (MQ=255)
                                                                                                                              cctccgtGGGGGAGCGGGCGGGGGTGNGGGCCTCAGCGCGCACGTTCACCCTCA‑CCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAG‑CACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAg                <  1:6350316/139‑1 (MQ=39)
                                                                                                                                   ctGTGGCAGTGGGCGGGGGTGAGGCCCTCAGCGCGCACGGTCACCCTCA‑CCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAG‑CACAAATTTTGAGAGATATCTTCTCCTGTCTTGTAACAGAAGAACAGAACAt           <  1:1130039/140‑1 (MQ=38)
                                                                                                                                           gagGGCCGGGGTGAGGGCCTCAGCGCGCACGTTCACCCTCA‑CCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAG‑CACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATCGGGCttt   <  2:6895244/140‑1 (MQ=255)
                                                                                                                                            |                                                                                                                                             
GAGCGTACCGTGAGGTGCAAAAAGAGATGAAAGGGCGTTATCCCAAACATG‑TCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCA‑CCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAG‑CACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATCGGGCTTTT  >  NZ_CP025268/164256‑164534

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑