BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP025268	541,580	G→A	A215T (GCG→ACG)	glxK →	glycerate 3‑kinase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP025268	541,580	0	G	A	99.3%	2018.1 / NA	547	A215T (GCG→ACG)	glxK	glycerate 3‑kinase
Reads supporting (aligned to +/- strand): ref base G (0/0); major base A (258/284); minor base C (1/3); total (259/288)
Fisher's exact test for biased strand distribution p-value = 6.26e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 554 total aligned reads displayed.

CGCGCGTGGCAAGCATTGAGATGGATGAATGCGATCCGCGTCTGGCGAATTGCCATATTGAAGTAGCATGTGACGTTGATAACCCGCTGGTAGGGGCACGCGGCGCGGCGGCGGTGTTTGGCCCGCAAAAAGGGGCAGCGCCGGAGATGGTCGAAGAACTTGAACAGGGGCTGCAAAATTACGCCCGTGTTTTACAACAGCAAACTGAAATTAATGTCTGCCAGATGGCGGGCGGCGGCGCTGCGGGCGGTATGGGTATTGCGGCGGCGGTA  >  NZ_CP025268/541443‑541714

cccccgaggCAAGCCTAGAGAAGCATGAATGCGATCCGCGTCTGGCGAATTTCCATATTGAAGCAGCATGTGACGTTGATAACCCGCTGGTAGGTGCACGCGGCGCGGCGGCGGTGTTTGGCCCGCAAAAAGGGGCAacg                                                                                                                                      <  1:7715084/133‑1 (MQ=255)

              aTTGAGATGGATGAATGCGATCCGCGTCTGGCGAATTGCCATATTGAAGTAGCATGTGACGTTGATAACCCGCTGGTAGGGGCACGCGGCGCGGCGGCGGTGTTTGGCCCGCAAAAAGGGGCAACGCCGGAGATGGTCga                                                                                                                        <  1:4814883/140‑1 (MQ=255)

              aTTGAGATGGAAGAATGCGATCCGCGTCTGGCGAATTGCCATATTGAAGTAGCATGTGACGTTGATAACCCGCTGGTAGGGGCACGCGGCGCGGCGGCGGTGTTTGGCCCGCAAAAAGGGGCAACGCCGGAGATGGTCga                                                                                                                        <  1:4682904/140‑1 (MQ=255)

               ttGAGATGGATGAATGCGATCCGCGTCTGGCGTATTGCCATATTGAAGTAGCATGTGACGTTGATAACCCGCTGGTAGGGGCACGCGGCGCGGCGGCGGTGTTTGGCCCGCAAAAAGGGGCAACGCCGGAGATGGTCgaa                                                                                                                       <  2:911471/140‑1 (MQ=255)

               ttGAGATGGATGAATGCGATCCGCGTCTGGCGAATTGCCATATTGAAGTAGCATGTGACGTTGATAACCCGCTGGTAGGGGCACGCGGCGCGGCGGCGGTGTTTGGCCCGCAAAATGGGGCAACGCCGGAGATGGTCgaa                                                                                                                       >  2:4019501/1‑140 (MQ=255)

                tGAGATGGATGAATGCGATCCGCGTCTGGCGAATTGCCATATTGAAGTAGCATGTGACGTTGATAACCCGCTGGTAGGGGCACGCGGCGCGGCGGCGGTGTTTGGCCCGCAAACAGGGGCAACGCCGGAGATGGTCgaag                                                                                                                      <  1:9159970/140‑1 (MQ=255)

                tGAGATGGATGAATGCGATCCGCGTCTGGCGAATTGCCATATTGAAGGAGCATGTGACGTTGATAACCCGCTGGTAGGGGCACGCGGCGCGGCGGCGGTGTTTGGCCCGCAAAAAGGGGCAACGCCGGAGATGGTCgaag                                                                                                                      <  2:2827850/140‑1 (MQ=255)

                 gagaTGGATGAATGCGATCCGCGTCTGGCGAATTGCCATATTGAAGTAGCATGTGACGTTGATCACCCGCTGGTAGGGGCACGCGGCGCGGCGGCGGTGTTTGGCCCGCAAAAAGGGGCAACGCCGGAGATGGTCgaaga                                                                                                                     <  1:8239999/140‑1 (MQ=255)

                 gagaTGGATGAATGCGATCCGCGTCTGGCGAATTGCCATATTGAAGTAGCATGTGACGTTGATAACCCGCTGGTAGGGGCACGCGGCGCGGCGGCGGTGTTTGGCCCGCAAAAAGGGGCAACGCCGGAGATGGTCgaaga                                                                                                                     >  1:2076452/1‑140 (MQ=255)

                       gctgAATGCGATCCGCGTCTGGCGAATTGCCAGAGTGAAGTAGCATGTGACGTTGAGAACCCGCTGGTAGGGGCCCGCGGCGCGGCGGCGGTGTTTGGCCCGCAAAAAGGGGCAACGCCGGAGAGGGTCGACGAACTTGa                                                                                                               >  2:5396172/3‑140 (MQ=255)

                       gatgAATGCGATCCGCGTCTGGCGAATTGCCATATTGAAGTAGCATGTGACGTTGATAACCCGCTGGTAGGGGCACGCGGCGCGGCGGCGGTGTTTGGCCCGCAAAAAGGGGCAACGCCGGAGATGGTCGAAGAACTTGa                                                                                                               >  1:4607234/1‑140 (MQ=255)

                        atgAATGCGATCCGCGTCTGGCGAATTGCCATATTGAAGTAGCATGTGACGTTGATAACCCGCTGGTAGGGGCACGCGGCGCGGCGGCGGTGTTTGGCCCGCAAAAAGGGGCAACGCCGGAGATGGTCGAAGTACTTGaa                                                                                                              >  1:1384022/1‑140 (MQ=255)

                        atgAATGCGATCCGCGTCTGGCGAATTGCCATATTGAAGTAGCATGTGACGTTGATAACCCGCTGGTAGGGGCACGCGGCGCGGCGGCGGTGTTTGGCCCGCAAAAAGGGGCAACGCCGGAGATGGTCGAAGAACTTGac                                                                                                              >  2:3795399/1‑139 (MQ=255)

                         tgAATGCGCTCCGCGTCTGGCGAATTGCCATATTGAAGTAGCATGTGACGTTGATAACCCGCTGGTAGGGGCACGCGGCGCGGCGGCGGTGTTTGGCCCGCAAAAAGGGGCAACGCCGGAGATGGTCGAAGAACTTGAAc                                                                                                             <  1:8387013/140‑1 (MQ=255)

                            aTGCGATCCGCGTCTGGCGAATTGCCATATTGAAGTAGCATGTGACGTTGATAACCCGCTGGTAGGGGCACGCGGCGCGGCGGCGGTGTTTGGCCCGCAAAAAGGGGCAACGCCGGAGATGGTCGAAGAACTTGAACAgg                                                                                                          >  1:8118454/1‑140 (MQ=255)

                              gCGATCCGCGTCTGGCGAATTGCCATATTGAAGTAGCATGTGACGTTGATAACCCGCTGGTAGGGGCACGCGGCGCGGCGGCGGTGTTTGGCCCGCAAAAAGGGGCAACGCCGGAGATGGTCGAAGAACTTGAACAgggg                                                                                                        <  1:9025413/140‑1 (MQ=255)

                                 aTCCGCGTCTGGCGAATTGCCATATTGAAGAAGCATGTGACGTTGATAACCCGCTGGTAGGGGCACGCGGCGCGGCGGCGGTGTTTGGCCCGCAAAAAGGGGCAACGCCGGAGATGGTCGAAGAACTTGAACAGGGGCTg                                                                                                     >  1:5848625/1‑140 (MQ=255)

                                  tCCGCGTCTGGCGAATTGCCATATTGTATTAGCATGTGACGTTGATAACCCGCTGGTAGGGGCACGCGGCGCGGCGGCGGTGTTTGGCCCGCAAAAAGGGGCAACGCCGGAGATGGTCGAAGAACTTGAACAGGGGCTGc                                                                                                    <  1:5552255/140‑1 (MQ=255)

                                  tCCGCGTCTGGCGAATTGCCATATTGAAGTAGCATGTGACGTTGATAACCCGCTGGTAGGGGCACGCGGAGCGGCGGCGGTGTTTGGCCCGCAAAAAGGGGCAACGCCGGAGATGGTCGAAGAACTTGAACAGGGGCTGc                                                                                                    <  2:2546776/140‑1 (MQ=255)

                                   ccGCGTCTGGCGAATTGCCATATTGAAGTAGCATGTGACGTTGATAACCCGCTGGTAGGGGCACGCGGCGCGGCGGCGGTGTTTGGCCCGCAAAAAGGGGCAACGCCGGAGATGGTCGAAGAACTTGAACAGGGGCTGCa                                                                                                   <  1:92846/140‑1 (MQ=255)

                                   ccGCGTCTGGCGAATTGCCATATTGAAGTAGCATGTGACGTTGATAACCCGCTGGTAGGGGCACGCGGCGCGGCGGCGGTGTTTGGCCCGCAAAAAGGGGCAACGCCGGAGATGGTCGAAGAACTTGAACAGGGGCTGCa                                                                                                   <  1:7874821/140‑1 (MQ=255)

                                    cgcgTCTGGCGAATTGCCATATTGAAGTAGCATGTGACGTTGATAACCCGCTGGTAGGGGCACGCGGCGCGGCGGCGGTGTTTGGCCCGCAAAAAGGGGCAACGCCGGAGATGGTCGAAGAACTTGAACAGGGGCTGCaa                                                                                                  >  2:4207002/1‑140 (MQ=255)

                                      cgTCTGGCGAATTGCCATATTGAAGTAGCATGTGACGTTGATAACCCGCTGGTAGGGGCACGCGGCGCGGCGGCGGTGTTTGGCCCGCAAAAAGGGGCAACGCCGGAGGTGGTCGAAGAACTTGAACAGGGGCTGCaaaa                                                                                                >  1:7543047/1‑140 (MQ=255)

                                            gCGAATTGCCATATTGAAGTAGCATGTGACGTTGATAACCCGCTGGTAGGGGCACGCGGCGCGGCGGCGGTGTTTGGCCCGCAAAAAGGGGCAACGCCGGAGATGGTCGAAGAACTTGAACAGGGGCTGCAAAATTACGc                                                                                          >  2:585231/1‑140 (MQ=255)

                                            gCGAATTGCCATATTGAAGTAGCATGTGACGATGATAACCCGCTGGTAGGGGCACGCGGCGCGGCGGCGGTGTTTGGCCCGCAAAAAGGGGCAACGCCGGAGATGGTCGAAGAACTTGAACAGGGGCTGCAAAATTACGc                                                                                          <  2:8973488/140‑1 (MQ=255)

                                             cgcaTTGCCAGATTGAAGTAGCATGTGACGTTGATAACCCGCTGGTAGGGGCACGCGGCGCGGCGGCGGTGTTTGGCCCGCAAAAAGGGGCAACGCCGGAGATGGTCGAAGAACTTGAACAGGGGCTGCAAAATTACGcc                                                                                         <  2:8571182/137‑1 (MQ=255)

                                               acttGCCATATTGAAGTAGCATGTGACGTTGATAACCCGCTGGTAGGGGCACGCGGCGCGGCGGCGGTGTTTGGCCCGCAAAAAGGGGCAACGCCGGAGATGGTCGAAGAACTTGAACAGGGGCTGCAAAATTACGCCCg                                                                                       <  1:9451903/138‑1 (MQ=255)

                                                 ttGCCATATTGAAGTAGCATGTGACGTTGTTAACCCGCGGGTAGGGGCACGCGGCGCGGCGGCGGTGTTTGGCCCGCAAAAAGGGGCAACGCCGGAGATGGTCGAAGAACTTGAACAGGGGCTGCAAAATTACGCCCgtg                                                                                     <  2:1765408/140‑1 (MQ=255)

                                                  tGCCATATTGAAGTAGCATGTGACGTTGATAACCCGCTGGTAGGGGCACGCGGCGCGGCGGCGGTGTTTGGCCCGCAAAAAGGGGCAACGCCGGAGATGGTCGAAGAACTTGAACAGGGGCTGCAAAATTACGCCCgtgt                                                                                    <  1:8726824/140‑1 (MQ=255)

                                                        atTGCAGGAGCATGTGACGTTGATAACCCGCTGGTAGGGGCACGCGGCGCGGCGGCGGTGTTTGGCCCGCAAAAAGGGGCAACGCCGGAGATGGTCGAAGAACTTGAACAGGGGCTGCAAAATTACGCCCGTGTTTTaca                                                                              <  1:2429257/140‑1 (MQ=255)

                                                        atTGAAGTAGCATGTGACGTTGATAACCCGCTGGTAGGGGCACGCGGCGCGGCGGCGGTGTTTGGCCCGCAAAAAGGGGCAACGCCGGAGATGGTCGAAGAACTTGAACAGGGGCTGCAAAATTACGCCCGTGTTTTaca                                                                              <  1:3383718/140‑1 (MQ=255)

                                                              gTAGCATGTGACGTTGATAACCCGCTGGTAGGGGCACGCGGCGCGGCGGCGGTGTTTGGCCCGCAAAAAGGGGCAACGCCGGAGATGGTCGAAGAACTTGAACAGGGGCTGCAAAATTACGCCCGTGTTTTACAACAGCa                                                                        >  1:8895403/1‑140 (MQ=255)

                                                                 gcttgtgTCGTTGATAACCCGCTGGGAGGGGCACGCGGCGCGGCGGCGGTGTTTGGCCAGCATATAGGGGCAACGCCGGAGATGGTCGATGAACTTGAACAGGGGCTGCAAAATTACGCCCGTGTTTTACAACATCAAAc                                                                     <  2:6311809/137‑1 (MQ=255)

                                                                 gCATGTGACGTTGATAACCCGCTGGTAGGGGCACGCGGCGCGGCGGCGGTGTTTGGCCCGCAAAAAGGGGCAACGCCGGAGATGGTCGAAGAACTTGAACAGGGGCTGCAAAATTACGCCCGTGTTTTACAACAGCAAAc                                                                     <  2:5715106/140‑1 (MQ=255)

                                                                 gCATGTGACGTTGATAACCCGCTGGTAGGGGCACGCGGCGCGGCGGCGGTGTTTGGCCCGCAAAAAGGGGCAACGCCGGAGATGGTCGAAGAACTTGAACAGGGGCTGCAAAATTACGCCCGTGTTTTACAACAGCAAAc                                                                     >  1:2742650/1‑140 (MQ=255)

                                                                 gCATGTGACGTTGATAACCCGCTGGTAGGGGCACGCGGCGCGGCGGCGGTGTTTGGCCCGCAAAAAGGGGCAACGCCGGAGATGGTCGAAGAACTTGAACAGGGGCTGCAAAATTACGCCCGTGTTTTACAACAGCAAAc                                                                     >  1:2965827/1‑140 (MQ=255)

                                                                 gCATGTGACGTTGATAACCCGCTGGTAGGGGCACGCGGCGCGGCGGCGGTGTTTGGCCCGCAAAAAGGGGCAACGCCGGAGATGGTCGAAGAACTTGAACAGGGGCTGCAAAATTACGCCCGTGTTTTACAACAGCAAAc                                                                     <  1:3984361/140‑1 (MQ=255)

                                                                 gCATGTGACGTTGATAACCCGCTGGTAGGGGCACGCGGCGCGGCGGCGGTGTTTGGCCCGCAAAAAGGGGCAACGCAGGAGATGGTCGAAGAACTTGAACAGGGGCTGCAAAATTACGCCCGTGTTTTACAACAGCAAAc                                                                     <  2:3234738/140‑1 (MQ=255)

                                                                  cATGTGACGTTGATAACCCGCTGGTAGGGGCACGCGGCGCGGCGGCGGTGTTTGGCCCGCAAAAAGGGGCAACGCCGGAGATGGTCGAAGAACTTGAACAGGGGCTGCAAAATTACGCCCGTGTTTTACAACAGCAAACt                                                                    >  2:1595195/1‑140 (MQ=255)

                                                                  cATGTGACGTTGATAACCCGCTGGTAGGGGCACGCGGCGCGGCGGCGGTGTTTGGCCCGCAAAAAGGGGCAACGCCGGAGATGGTCGAAGAACTTGAACAGGGGCTGCAAAATTACGCCCGAGTATTACAACAGCAAACt                                                                    >  1:6044361/1‑140 (MQ=255)

                                                                   aTGTGACGTTGATAACCCGCTGGTAGGGGCACGCGGCGCGGCGGCGGTGTTTGGCCCGCAAAAAGGGGCAACGCCGGAGATGGTCGAAGAACTTGAACAGGGGCTGCAAAATTACGCCCGTGTTTTACAACAGCAAACTg                                                                   >  1:740306/1‑140 (MQ=255)

                                                                   aTGTGACGTTGATAACCCGCTGGTAGGGGCACGCGGCGCGGCGGCGGTGTTTGGCCCGCAAAAAGGGGCAACGCCGGAGATGGTCGAAGAACTTGAACAGGGGCTGCAAAATAACGCCCGTGATTTACAACAGCAAACTg                                                                   >  1:6295647/1‑140 (MQ=255)

                                                                      tgACGTTGATAACCCGCTGGTAGGGGCACGCGGCGCGGCGGCGGTGTTTGGCCCGCAAAAAGGGGCAACGCCGGAGATGGTCGAAGAACTTGAACAGGGGCTGCAAAATTACGCCCGTGTTTTACAACAGCAAACTGaaa                                                                >  2:195786/1‑140 (MQ=255)

                                                                      tgACGTTGATAACCCGCTGGTAGGGGCACGCGGCGCGGCGGCGGTGTTTGGCCCGCAAAAAGGGGCAACGCCGGAGATGGTCGAAGAACTTGAACAGGGGCTGCAAAATTACGCCCGTGTTTTACAACAGCAAACTGaaa                                                                >  2:7699684/1‑140 (MQ=255)

                                                                      tgACGTTGATAACCCGCTGGTAGGGGCACGCGGCGCGGCGGCGGTGTTTGGCCCGCAAAAAGGGGCAACGCCGGAGATGGTCGAAGAACTTGAACAGGGGCTGCAAAATTACGCCCGTGTTTTACAACAGCAAACTGaaa                                                                >  1:4325848/1‑140 (MQ=255)

                                                                         cGTTGATAACCCGCTGGTAGGGGCACGCGGCGCGGCGGCGGTGTTTGGCCCGCAAAAAGGGGCAACGCCGGAGATGGTCGAAGAACTTGAACAGGGGCTGCAAAATTACGCCCGTGTTTTACAACAGCAAACTGAAATTa                                                             <  2:6808637/140‑1 (MQ=255)

                                                                           ttGATAACCCGCTGGTAGGGGCACGCGGCGCGGCGGCGGTGTTTGGCCCGCAAAAAGGGGCAACGCCGGAGATGGTCGAAGAACTTGAACAGGGGCTGCAAAATTACGCCCGTGTTTTACAACAGCAAACTGAAATTAAt                                                           >  1:7233281/1‑140 (MQ=255)

                                                                           ttGATAACCCGCTGGTAGGGGCACGCGGCGCGGCGGCGGTGTTTGGCCCGCAAAAAGGGGCAACGCCGGAGATGGTCGAAGAACTTGAACAGGGGCTGCAAAATTACGCCCGTGTTTTACAACAGCAAACTGAAATTAAt                                                           <  1:4522314/140‑1 (MQ=255)

                                                                            tGATAACCCGCTGGTAGGGGCACGCGGCGCGGCGGCGGTGTTTGGCCCGCAAAAAGGGGCAACGCCGGAGATGGTCGAAGAACTTGAACAGGGGCTGCAAAATTACGCCCGTGTTTTACAACAGCAAACTGAAATTAATg                                                          >  1:2287941/1‑140 (MQ=255)

                                                                             gATAACCCGCTGGTAGGGGCACGCGGCGCGGCGGCGGTGTTTGGCCCGCAAAAAGGGGCAACGCCGGAGATGGTCGAAGAACTTGAACAGGGGCTGCAAAATTACGCCCGTGTTTTACAACAGCAAACTGAAATTAATGt                                                         >  1:581377/1‑140 (MQ=255)

                                                                              aTAACCCGCTGGTAGGGGCACGCGGCGCGGCGGCGGTGTTTGGCCCGCAAAAAGGGGCAACTCCGGAGATGGTCGAAGAACTTGAACAGGGGCTGCAAAATTACGCCCGTGTTTTACAACAGCAAACTGAAATTAATGTc                                                        >  2:6046240/1‑140 (MQ=255)

                                                                              aTAACCCGCTGGTAGGGGCACGCGGCGCGGCGGCGGTGTTTGGCCCGCAAAAAGGGGCAACGCCGGAGATGGTCGAAGAACTTGAACAGGGGCTGCAAAATTACGCCCGTGTTTTACAACAGCAAACTGAAATTAATGTc                                                        >  2:8011313/1‑140 (MQ=255)

                                                                              aTAACCCGCTGGTAGGGGCACGCGGCGCGGCGGCGGTGTTTGGCCCGCAAAAAGGGGCAACGCCGGAGATGGTCGAAGAACTTGAACAGGGGCTGCAAAATTACGCCCGTGTTTTACAACAGCAAACTGAAATTAATGTc                                                        >  1:1111090/1‑140 (MQ=255)

                                                                                aaCCCGCTGGTAGGGGCACGCGGCGCGGCGGCGGTGTTTGGCCCGCAAAAAGGGGCAACGCCGGAGATGGTCGAAGAACTTGAACAGGGGCTGCAAAATTACGCCCGTGTTTTACAACAGCAAACTGAAATTAATGTCTg                                                      >  1:104962/1‑140 (MQ=255)

                                                                                 aCCCGCTGGTAGGGGCACGCGGCGCGGCGGCGGTGTTTGGCCCGCAAAAAGGGGCAACGCCGGAGATGGTCGAAGAACTTGAACAGGGGCTGCAAAATTACGCCCGTGTTTTACAACAGCAAACTGAAATTAATGTCTGc                                                     <  1:2574326/140‑1 (MQ=255)

                                                                                 aCCCGCTGGTAGGGGCACGCGGCGCGGCGGCGGTGTTTGGCCCGCAAAAAGGGGCAACGCCGGAGATGGTCGAAGAACTTGAACAGGGGCTGCAAAATTACGCCCGTGTTTTACAACAGCAAACTGAAATTAATGTCTGc                                                     <  2:2024721/140‑1 (MQ=255)

                                                                                   ccGCTGGTAGGGGCACGCGGCGCGGCGGCGGTGTTTGGCCCGCAAAAAGGGGCAACGCCGGAGATGGTCGAAGAACTTGAACAGGGGCTGCAAAATTACGCCCGTGTTTTACAACAGCAAACTGAAATTAATGTCTGCCa                                                   >  1:6478671/1‑140 (MQ=255)

                                                                                   ccGCTGGTAGGGGCACGCGGCGCGGCGGCGGTGTTTGGCCCGCAAAAAGGGGCAACGCCGGAGATGGTCGAAGAACTTGAACAGGGGCTGCAAAATTACGCCCGTGTTTTACAACAGCAAACTGAAAGTAATGTCAGCCa                                                   >  1:3672350/1‑140 (MQ=255)

                                                                                     gCTGGTAGGGGCACGCGGCGCGGCGGCGGTGTTTGGCCCGCAAAAAGGGGCAACGCCGGAGATGGTCGAAGAACTTGAACAGGGGCTGCAAAATTACGCCCGTGTTTTACAACAGCAAACTGAAATTAATGTCTGCCAGa                                                 >  2:7088039/1‑140 (MQ=255)

                                                                                     gCTGGTAGGGGCACGCGGCGCGGCGGCGGTGTTTGGCCCGCAAAAAGGGGCAACGCCGGAGATGGTCGAAGAACTTGAACAGGGGCTGCAAAATTACGCCCGTGTTTTACAACAGCAAACTGAAATTAATGTCTGCCAGa                                                 >  1:3258617/1‑140 (MQ=255)

                                                                                     gCTGGTAGGGGCACGCGGCGCGGCGGCGGTGTTTGGCCCGCAAAAAGGGGCAACGCCGGAGATGGTCGAAGAACTTGAACAGGGGCTGCAAAATTACGCCCGTGTTTTACAACAGCAAACTGAAATTAATGTCTGCCAGa                                                 >  1:6673103/1‑140 (MQ=255)

                                                                                      cTGGTAGGGGCACGCGGCGCGGCGGCGGTGTTTGGCCCGCAAAAAGGGGCAACGCCGGAGATGGTCGAAGACCTTGAACAGGGGCTGCAAAATTACGCCCGTGTTTTACAACAGCAAACTGAAATTAATGTCTGCCAGAt                                                >  2:1341300/1‑140 (MQ=255)

                                                                                        ggTAGGGGCACGCGGCGCGGCGGCGGTGGTTGGCCCGCAAAAAGGGGCAACGCCGGAGATGGTCGAAGAACTGGAACAGGGGCTGCAAAATTACGCCCGTGTTTTACAACAGCAAACTGAAATTAATGTCTGCCAGATgg                                              >  2:4575343/1‑140 (MQ=255)

                                                                                          tAGGGGCACGCGGCGCGGCGGCGGTGTTTGGCCCGCAAAAAGGGGCAACGCCGGAGATGGTCGAAGAACTTGAACAGGGGCTGCAAAATTACGCCCGTGTTTTACAACAGCAAACTGAAATTAATGTCTGCCTGATggcg                                            <  1:8804459/140‑1 (MQ=255)

                                                                                            ggggCACGCGGCGCGGCGGCGGTGTTTGGCCCGCAAAAAGGGGCAACGCCGGAGATGGTCGAAGAACTTGAACAGGGGCTGCAAAATTACGCCCGTGTTTTACAACAGCAAACTGAAATTAATGTCTGCCAGATggcggg                                          <  1:1745642/140‑1 (MQ=255)

                                                                                            ggggCACGCGGCGCGGCGGCGGTGTTTGGCCCGCAAAAAGGGGCAACGCCGGAGATGGTCGAAGAACTTGAACAGGGGCTGCAAAATTACGCCCGTGTTTTACAACAGCAAACTGAAATTAATGTCTGCCAGATggcggg                                          <  2:2300860/140‑1 (MQ=255)

                                                                                            ggggCACGCGGCGCGGCGGCGGTGTTTGGCCCGCAAAAAGGGGCAACGCCGGAGATGGTCGAAGAACTTGAACAGGGGCTGCAAAATTACGCCCGTGTTTTACAACAGCAAACTGAAATTAATGTCTGCCAGATggcggg                                          <  1:3421745/140‑1 (MQ=255)

                                                                                             gggCACGCGGCGCGGCGGCGGTGTTTGGCCCGCAAAAAGGGGCAACGCCGGAGATGGTCGAAGAACTTGAACAGGGGCTGCAAAATTACGCCCGTGTTTTACAACAGCAAACTGAAATTAATGTCTGCCAGATggcgggc                                         >  1:8676215/1‑140 (MQ=255)

                                                                                               gCACGCGGCGCGGCGGCGGTGTTTGGCCCGCAAAAAGGGGCAACGCCGGAGATGGTCGAAGAACTTGAACAGGGGCTGCAAAATTACGCCCGTGTTTTACACCAGCAAACTGAAATTAATGTCTGCCAGATGgcgggcgg                                       >  2:3016763/1‑140 (MQ=255)

                                                                                                 aCGCGGCGCGGCGGCGGTGTTTGGCCCGCAAAAAGGGGCAACGCCGGAGAGGGTCGAAGAACTTGAACAGGGGCTGCAAAATTACGCCCGTGTTTTACAACAGCAAACTGAAATTAATGTCTGCCAGATGGCGggcggcg                                     <  1:1907260/140‑1 (MQ=255)

                                                                                                   gcggcgcggcGGCGGTGTTTGGCCCGCAAAAAGGGGCAACGCCGGAGATGGTCGAAGAACTTGAACAGGGGCTGCAAAATTACGCCCGTGTTTTACAACAGCAAACTGAAATTAATGTCTGCCAGATGGCGggcggcggc                                   >  1:373687/1‑140 (MQ=255)

                                                                                                   gcggcgcggcGGCGGTGTTTGGCCCGCAAAAAGGGGCAACGCCGGAGATGGTCGAAGAACTTGAACAGGGGCTGCAAAATTACGCCCGTGTTTTACAACAGCAAACTGAAATTAATGTCTGCCAGATGGCGggcggcggc                                   >  2:506362/1‑140 (MQ=255)

                                                                                                   gcggcgcggcGGCGGTGTTTGGCCCGCAAAAAGGGGCAACGCCGGAGATGGTCGAAGAACTTGAACAGGGGCTGCAAAATTACGCCCGTGTTTTACAACAGCAAACTGAAATTAATGTCTGCCAGATGGCGggcggcggc                                   >  1:5362263/1‑140 (MQ=255)

                                                                                                         cgggggcggTGTTTGGCGCTCAAACAGGGTCAACGCCGGAGTTGGTCGAAGACCTTGAACAGGGGCTGAAACATTTCGCCCGTTTTTACCAACAGCACACTGAATTTAAGGTCTGCCAGATGGCGGGCTGCGGCTCTtcg                             <  2:9328651/140‑1 (MQ=255)

                                                                                                         cggcggcggTGTTTGGCCCGCAAAAAGGGGCAACGCCGGAGATGGTCGAAGAACTTGAACAGGGGCTGCAAAATTACGCCCGTGTTTTACAACAGCAAACTGAAATTAATGTCTGCCAGATGGCGGGCGGCGGCGCTgcg                             >  1:8006714/1‑140 (MQ=255)

                                                                                                          gtcggctgTGTTTGGCCCGCAAAAAGGTGCAACGCCGTAGATGGTCGAAGTAGTTGTACAGGGGCTGCTAACTTTCGCCCGTGTTTTACAACAGCAAACTGAAATTTAGGTCTTCCTGATGGCGGGCGGCGGCGCTgcgg                            <  2:7550833/138‑1 (MQ=255)

                                                                                                          ggcggcggTGTTTGGCCCGCAAAAAGGGGCAACGCCGGAGATGGTCGAAGAACTTGAACAGGGGCTGCAAAATTACGCCCGTGTTTTACAACAGCAAACTGAAATTAATGTCTGCCAGATGGCGGGCGGCGGCGCTgcgg                            <  2:5217794/140‑1 (MQ=255)

                                                                                                          ggcggcggTGTTTGGCCCGCAAAAAGGGGCAACGCCGGAGATGGTCGAAGAACTTGAACAGGGGCTGCAAAATTACGCCCGTGTTTTACAACAGCAAACTGAAATTAATGTCTGCCAGATGGCGGGCGGCGGCGCTgcgg                            <  2:8751188/140‑1 (MQ=255)

                                                                                                           gcggcggTGTTTGGCCCGCCAACAGGGGCAACGCCGGAGATGGTCGAAGAACTTGAACAGGGGCTGCAAAATTACGCCCGTGTTGTACAACAGCAAACTGAACTTAATGTCTGCCAGATGGCGGGCGGCGGCGCTgcggg                           <  2:4957497/140‑1 (MQ=255)

                                                                                                           gcggcggTGTTTGGCCCGCAAAAAGGGGCAACGCCGGAGATGGTCGAAGAACTTGAACAGGGGCTGCAAAATTACGCCCGTGTTTTACAACAGCAAACTGAAATTAATGTCTGCCAGATGGCGGGCGGCGGCGCTgcggg                           <  2:3908781/140‑1 (MQ=255)

                                                                                                           gcggcggTGTTTGGCCCGCAAAAAGGGGCAACGCCGGAGATGGTCGAAGAACTTGAACAGGGGCTGCAAAATTACGCCCGTGTTTTACAACAGCAAACTGAAATTAATGTCTGCCAGATGGCGGGCGGCGGCGCTgcggg                           <  1:4207002/140‑1 (MQ=255)

                                                                                                           gcggcggTGTGTGGCCCGCAAAAAGGGGCAACGCCGGAGAGGGTCGAAGAACTTGAACAGGGGCTGCAAAATTACGCCCGTGTTTTACAACAGCAAACTGAAATTAAGGTCTGCCAGATGGCGGGCGGCGGCGCTgcggg                           <  1:8594316/140‑1 (MQ=255)

                                                                                                           gcggcggTGATTGGCCCGCAAAAAGGGGCAACGCCGGAGATGGTCGAAGAACTTGAACAGGGGCTGCAAAATTACGCCCGTGTTTTACAACAGCAAACTGAAATTAATGTCTGCCAGATGGCGGGCGGCGGCGCTgcggg                           <  1:5094389/140‑1 (MQ=255)

                                                                                                            cggcggTGTTTGGCCCGCTAATAGGGGCAACGCCGGAGATGGTCGAAGAACTTGAACAGGGGCTGCAAAATTCCGCCCGTGTTTTACAACAGCAAACTGAAATTAATGTCTGCCAGATGGCGGGCGGCGGCGCTgcgggc                          <  1:851481/140‑1 (MQ=255)

                                                                                                            cggcggTGTTTGGCCCGCAAAAAGTGGCAACGCCGGAGATTGTCTAAGAACTTGAACAGGGGCTGCAAAATTTCGCCCGTGTTTTACAACATCATACTGAAATTAATGTCTGCCAGATGGCGGGCGGCGGCGCTgcgggc                          <  2:3353843/140‑1 (MQ=255)

                                                                                                            cggcggTGTTTGGCCCGCAAAAAGGGGCAACGCCGGAGATGGTCGAAGAACTTGAACAGGGGCTGCAAAATTACGCCCGTGTTTTACAACAGCAAACTGAACTTAATGTCTGCCAGATGGCGGGCGGCGGCGCTgcgggc                          <  2:8263903/140‑1 (MQ=255)

                                                                                                            cggcggTGTTTGGCCCGCAAAAAGGGGCAACGCCGGAGATGGTCGAAGAACTTGAACAGGGGCTGCAAAATTACGCCCGTGTTTTACAACAGCAAACTGAAATTAATGTCTGCCAGATGGCGGGCGGCGGCGCTgcgggc                          <  2:1125648/140‑1 (MQ=255)

                                                                                                            cggcggTGTTTGGCCCGCAAAAAGGGGCAACGCCGGAGATGGTCGAAGAACTTGAACAGGGGCTGCAAAATTACGCCCGTGTTTTACAACAGCAAACTGAAATTAATGTCTGCCAGATGGCGGGCGGCGGCGCTgcgggc                          <  2:4375907/140‑1 (MQ=255)

                                                                                                            cggcggTGTTTGGCCCGCAAAAAGGGGCAACGCCGGAGATGGTCGAAGAACTTGAACAGGGGCTGCAAAATTACGCCCGTGTTTTACAACAGCAAACTGAAATTAATGTCTGCCAGATGGCGGGCGGCGGCGCTgcgggc                          <  2:1265527/140‑1 (MQ=255)

                                                                                                            cggcggTGTTTGGCCCGCAAAAAGGGGCAACGCCGGAGATGGTCGAAGAACTTGAACAGGGGCTGCAAAATTACGCCCGTGTTTTACAACAGCAAACTGAAATTAATGTCTGCCAGATGGCGGGCGGCGGCGCTgcgggc                          <  1:6957635/140‑1 (MQ=255)

                                                                                                               cggTGTTTGGCCCGCAAAAAGGGGCAACGCCGGAGATGGTCGAAGAACTTGAACAGGGGCTGCAAAATTACGCCCGTGTTTTACAACAGCAAACTGAAATTAATGTCTGCCAGATGGCGGGCGGCGGCGCTGCGGGCGGt                       >  1:6805710/1‑140 (MQ=255)

                                                                                                                ggTGTTTGGCCCGCAAAAAGGGGCAACGCCGGAGATGGTCGAAGAACTTGAACAGGGGCTGCAAAATTACGCCCGTGTTTTACAACAGCAAACTGAAATTAATGTCTGCCAGATGGCGGGCGGCGGCGCTGCGGGCGGTa                      <  2:8895403/140‑1 (MQ=255)

                                                                                                                 gtgtTTGGCCCGCAAATAGGGGCAACGCCGGAGATGGTCGAAGAACTTGAACAGGGGCTGCATTATTACGCCCGTGTTTTACAACAGCAATCTGACATTAATGTCTGCCAGATGGCGGGCGGCGGCGCTGCGGGCGGTAt                     <  1:6930593/140‑1 (MQ=255)

                                                                                                                            gCAAAAAGGGGCAACGCCGGAGATGGTCGAAGAACTTGAACAGGGGCTGCAAAATTACGCCCGTGTTTTACAACAGCAAACTGAAATTAATGTCTGCCAGATGGCGGGCGGCGGCGCTGCGGGCGGTATGGGTATTgcgg          >  1:1201173/1‑140 (MQ=255)

                                                                                                                            gCAAAAAGGGGCAACGCCGGAGATGGTCGAAGAACTAGAACCGGGGCTGCAAAATTACGCCCGTGTTTTACAACAGCAAACTGAAATTAATGTCTGCCAGATGGCGGGCGGCGGCGCTGCGGGCGGTATGGGTATTgcgg          <  2:7821095/140‑1 (MQ=255)

                                                                                                                               acccggggCCACGCCGGAGATGGTCGAAGAACTTGAACCGGGGCTGCAAAATTACGCCCGGGTTATACAACAGCAACCTGAAATTAATGTCTTCCAGATGGCGGGCGGCGGCGCTGCGGGCGGTATGGGTATTgcggcgg       <  2:3673/136‑1 (MQ=255)

                                                                                                                               aaaaGGGGCAACGCCGGAGATGGTCGAAGAACTTGAACAGGGGCTGCAAAATTACGCCCGTGTTTTACAACAGCAAACTGAAATTAATGTCTGCCAGATGGCGGGCGGCGGCGCTGCGGGCGGTATGGGTATTgcggcgg       <  2:265705/140‑1 (MQ=255)

                                                                                                                               aaaaGGGGCAACGCCGGAGATGGTCGAAGAACTTGAACAGGGGCTGCAAAATTACGCCCGTGTTTTACAACAGCAAACTGAAATTAATGTCTGCCAGATGGCGGGCGGCGGCGCTGCGGGCGGTATGGGTATTgcggcgg       <  2:5280172/140‑1 (MQ=255)

                                                                                                                                    gggCAACGCCGGAGATGGTCGAAGAACTTGAACAGGGGCTGCAAAATTACGCCCGTGTTTTACAACAGCAAACTGAAATTAATGTCTGCCAGATGGCGGGCGGCGGCGCTGCGGGCGGTATGGGTATTGCGGCGGCGGTa  >  2:6588196/1‑140 (MQ=255)

                                                                                                                                    gggCAACGCCGGAGATGGTCGAAGAACTTGAACAGGGGCTGCAAAATTACGCCCGTGTTTTACAACAGCAAACTGAAATTAATGTCTGCCAGATGGCGGGCGGCGGCGCTGCGGGCGGTATGGGTATTGCGGCGGCGGTa  <  2:4684604/140‑1 (MQ=255)

CGCGCGTGGCAAGCATTGAGATGGATGAATGCGATCCGCGTCTGGCGAATTGCCATATTGAAGTAGCATGTGACGTTGATAACCCGCTGGTAGGGGCACGCGGCGCGGCGGCGGTGTTTGGCCCGCAAAAAGGGGCAGCGCCGGAGATGGTCGAAGAACTTGAACAGGGGCTGCAAAATTACGCCCGTGTTTTACAACAGCAAACTGAAATTAATGTCTGCCAGATGGCGGGCGGCGGCGCTGCGGGCGGTATGGGTATTGCGGCGGCGGTA  >  NZ_CP025268/541443‑541714

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`