BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP025268	745,775	A→G	264 (TAA→TAG) A348A (GCT→GCC)	nei → abrB ←	endonuclease VIII AbrB family transcriptional regulator

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP025268	745,775	0	A	G	99.5%	2003.7 / NA	580	264 (TAA→TAG) A348A (GCT→GCC)	nei abrB	endonuclease VIII AbrB family transcriptional regulator
Reads supporting (aligned to +/- strand): ref base A (0/0); major base G (273/304); minor base T (1/2); total (274/306)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 580 total aligned reads displayed.

ATAAGCATCATGGGGCGCTGTTTCGCTTTAAGGTTTTTCATCGAGATGGCGAACCGTGCGAACGTTGTGGCAGCATCATTGAGAAAACCACGCTGTCATCTCGCCCGTTTTACTGGTGCCCTGGCTGCCAGCATTAAGCCGACCGCTTCGGCGCATAGGTTGAAATAAACCGCGCAATGGCAGGCCCTGTCAGCAAAATACTGAACAGGCGTAGGGTTTGCATCGCCATAATGAGCGCCATATCGGCATTGCTCCCTGCGGCGATGACCG  >  NZ_CP025268/745639‑745908

aTAAGCATCATGGGGCGCTGTTTCGCTTTAAGGTTTTTCATCGAGATGGCGAACCGTGCGAACGTTGTGGCAGCATCATTGAGAAAACCACGCTGTCATCTCGCCCGTTTTACTGGTGCCCTGGCTGCCAGCACTAGGcc                                                                                                                                    >  2:8537851/1‑140 (MQ=255)

   aGCATCATGGGGCGCTGTTTCGCTTTAAGGTTTTTCATCGAGATGGCGAACCGTGCGAACGTTGTGGCAGCATCATTGAGAAAACCACGCTGTCATCTCGCCCGTTTTACTGGTGCCCTGGCTGCCAGCACTAGGCCGAc                                                                                                                                 >  1:8935161/1‑140 (MQ=255)

   aGCATCATGGGGCGCTGTTTCGCTTTAAGGTTTTTCATCGAGATGGCGAACCGTGCGAACGTTGTGGCAGCATCATTGAGAAAACCACGCTGTCATCTCGCCCGTTTTACTGGTGCCCTGGCTGCCAGCACTAGGCCGAc                                                                                                                                 <  2:4931465/140‑1 (MQ=255)

     catcatGGGGCGCTGTTTCGCTTTAAGGTTTTTCATCGAGATGGCGAACCGTGCGAACGTTGTGGCAGCATCATTGAGAAAACCACGCTGTCATCTCGCCCGTTTTACTGGTGCCCTGGCTGCCAGCACTAGGCCGACCg                                                                                                                               <  2:5882151/140‑1 (MQ=255)

       tcatGGGGCGCTGTTTCGCTTTAAGGTTTTTCATCGAGATGGCGAACCGTGCGAACGTTGTGGCAGCATCATTGAGAAAACCACGCTGTCATCTCGCCCGTTTTACTGGTGCCCTGGCTGCCAGCACTAGGCCGACCGCt                                                                                                                             >  1:799161/1‑140 (MQ=255)

        catGGGGCGCTGTTTCGCTTTAAGGTTTTTCATCGAGATGGCGAACCGTGCGAACGTTGTGGCAGCATCATTGAGAAAACCACGCTGTCATCTCGCCCGTTTTACTGGTGCCCTGGCTGCCAGCACTAGGCCGACCGCtt                                                                                                                            >  2:3018234/1‑140 (MQ=255)

        catGGGGCGCTGTTTCGCTTTAAGGTTTTTCATCGAGATGGCGAACCGTGCGAACGTTGTGGCAGCATCATTGAGAAAACCACGCTGTCATCTCGCCCGTTTTACTGGTGCCCTGGCTGCCAGCACTAGGCCGACCGCtt                                                                                                                            >  2:4692135/1‑140 (MQ=255)

         atGGGGCGCTGTTTCGCTTTAAGGTTTTTCATCGAGATGGCGAACCGTGCGAACGTTGTGGCAGCATCATTGAGAAAACCACGCTGTCATCTCGCCCGTTTTACTGGTGCCCTGGCTGCCAGCACTAGGCCGACCGCtgc                                                                                                                           >  2:6145067/1‑138 (MQ=255)

         atGGGGCGCTGTTTCGCTTTAAGGTTTTTCATCGAGATGGCGAACCGTGCGAACGTTGTGGCAGCATCATTGAGAAAACCACGCTGTCATCTCGCCCGTTTTACTGGTGCCCTGGCTGCCAGCACTAGGCCGACCGCTTc                                                                                                                           >  2:1466895/1‑140 (MQ=255)

         atGGGGCGCTGTTTCGCTTTAAGGTTTTTCATCGAGATGGCGAACCGTGCGAACGTTGTGGCAGCATCATTGAGAAAACCACGCTGTCATCTCGCCCGTTTTACTGGTGCCCTGGCTGCCAGCACTAGGCCGACCGCTTc                                                                                                                           >  1:9521193/1‑140 (MQ=255)

         atGGGGCGCTGTTTCGCTTTAAGGTTTTTCATCGAGATGGCGAACCGTGCGAACGTTGTGGCAGCATCATTGAGAAAACCACGCTGTCATCTCGCCCGTTTTACTGGTGCCCTGGCTGCCAGCACTAGGCCGACCGCTTc                                                                                                                           >  1:5340076/1‑140 (MQ=255)

         atGGGGCGCTGTTTCGCTTTAAGGTTTTTCATCGAGATGGCGAACCGTGCGAACGTTGTGGCAGCATCATTGAGAAAACCACGCTGTCATCTCGCCCGTTTTACTGGTGCCCTGGCTGCCAGCACTAGGCCGACCGCTTc                                                                                                                           >  1:4171726/1‑140 (MQ=255)

         atGGGGCGCTGTTTCGCTTTAAGGTTTTTCATCGAGATGGCGAACCGTGCGAACGTTGTGGCAGCATCATTGAGAAAACCACGCTGTCATCTCGCCCGTTTTACTGGTGCCCTGGCTGCCAGCACTAGGCCGACCGCTTc                                                                                                                           >  1:4015510/1‑140 (MQ=255)

            gggCGCTGTTTCGCTTTAAGGTTTTTCATCGAGATGGCGAACCGTGCGAACGTTGTGGCAGCATCATTGAGAAAACCACGCTGTCATCTCGCCCGTTTTACTGGTGCCCTGGCTGCCAGCACTAGGCCGACCGCTTCGgc                                                                                                                        >  2:1306376/1‑140 (MQ=255)

               cgcTGTTTCGCTTTAAGGTTTTTCATCGAGATGGCGAACCGTGCGAACGTTGTGGCAGCATCATTGAGAAAACCACGCTGTCATCTCGCCCGTTTTTCTGGTGCCCTGGCTGCCAGCACTAGGCCGACCGCTTCGGCGCa                                                                                                                     >  1:6016030/1‑140 (MQ=255)

               cgcTGTTTCGCTTTAAGGTTTTTCATCGAGATGGCGAACCGTGCGAACGTTGTGGCAGCATCATTGAGAAAACCACGCTGTCATCTCGCCCGTTTTACTGGTGCCCTGTCTGCCAGCACTAGGCCGACCGCTTCGGCGCa                                                                                                                     <  2:6945851/140‑1 (MQ=255)

               cgcTGTTTCGCTTTAAGGTTTTTCATCGAGATGGCGAACCGTGCGAACGTTGTGGCAGCATCATTGAGAAAACCACGCTGTCATCTCGCCCGTTTTACTGGTGCCCTGGCTGCCAGCACTAGGCCGACCGCTTCGGCGCa                                                                                                                     >  1:8825619/1‑140 (MQ=255)

               cgcTGTTTCGCTTTAAGGTTTTTCATCGAGATGGCGAACCGTGCGAACGTTGTGGCAGCATCATTGAGAAAACCACGCTGTCATCTCGCCCGTTTTACTGGTGCCCTGGCTGCCAGCACTAGGCCGACCGCTTCGGCGCa                                                                                                                     >  1:5674358/1‑140 (MQ=255)

               cgcTGTTTCGCTTTAAGGTTTTTCATCGAGATGGCGAACCGTGCGAACGTGGTGGCAGCATCATTGAGAAAACCACGCTGTCATCTCGCCCGTTTTACTGGTGCCCTGGCTGCCAGCACTAGGCCGACCGCTTCGGCGCa                                                                                                                     <  2:5231651/140‑1 (MQ=255)

                gcTGTTTCGCTTTAAGGTTTTTCATCGAGATGGCGAACCGTGCGAACGTTGTGGCAGCATCATTGAGAAAACCACGCTGTCATCTCGCCCGTTTTACTGGTGCCCTGGCTGCCAGCACTAGGCCGACCGCTTCGGCGCAt                                                                                                                    >  1:8592845/1‑140 (MQ=255)

                 cTGTTTCGCTTTAAGGTTTTTCATCGAGATGGCGAACCGTGCGAACGTTGTGGCAGCATCATTGAGAAAACCACGCTGTCATCTCGCCCGTTTTACTGGTGCCCTGGCTGCCAGCACTAGGCCGACCGCTTCGGCGCATa                                                                                                                   <  2:3273118/140‑1 (MQ=255)

                   gTTTCGCTTTAAGGTTTTTCATCGAGATGGCGAACCGTGCGAACGTTGTGGCAGCATCATTGAGAAAACCACGCTGTCATCTCGCCCGTTTTACTGGTGCCCTGGCTGCCAGCACTAGGCCGACCGCTTCGGCGCATAgg                                                                                                                 <  2:3418146/140‑1 (MQ=255)

                   gTTTCGCTTTAAGGTTTTTCATCGAGATGGCGAACCGTGCGAACGTTGTGGCAGCATCATTGAGAAAACCACGCTGTCATCTCGCCCGTTTTACTGGTGCCCTGGCTGCCAGCACTAGGCCGACCGCTTCGGCGCATAgg                                                                                                                 <  1:3609136/140‑1 (MQ=255)

                   gTTTCGCTTTAAGGTTTTTCATCGAGATGGCGAACCGTGCGAACGTTGTGGCAGCATCATTGAGAAAACCACGCTGTCATCTCGCCCGTTTTACTGGTGCCCTGGCTGCCAGCACTAGGCCGACCGCTTCGGCGCATAgg                                                                                                                 <  1:6770848/140‑1 (MQ=255)

                   gTTTCGCTTTAAGGTTTTTCATCGAGATGGCGAACCGTGCGAACGTTGTGGCAGCATCATTGAGAAAACCACGCTGTCATCTCGCCCGTTTTACTGGTGCCCTGGCTGCCAGCACTAGGCCGACCGCTTCGGCGCATAgg                                                                                                                 <  2:4823780/140‑1 (MQ=255)

                    tttCGCTTTAAGGTTTTTCATCGAGATGGCGAACCGTGCGAACGTTGTGGCAGCATCATTGAGAAAACCACGCTGTCATCTCGCCCGTTTTACTGGTGCCCTGGCTGCCAGCACTAGGCCGACCGCTTCGGCGCATAGGt                                                                                                                >  2:7482731/1‑140 (MQ=255)

                    ttgcGCTTTAAGGTTTTTCATCGAGATGGCGAACCGTGCGAACGTTGTGGCAGCATCATTGAGAAAACCACGCTGTCATCTCGCCCGTTTTACTGGTGCCCTGGCTGCCAGCACTAGGCCGACCGCTTCGGCGCATAGGt                                                                                                                >  2:4050331/4‑140 (MQ=255)

                         cTTTAAGGTTTTTCATCGAGATGGCGAACCGTGCGAACGTTGTGGCAGCATCATTGAGAAAACCACGCTGTCATCTCGCCCGTTTTACTGGTGCCCTGGCTGCCAGCACTAGGCCGACCGCTTCGGCGCATAGGTTGaaa                                                                                                           >  1:3271273/1‑140 (MQ=255)

                         cTTTAAGGTTTTTCATCGAGATGGCGAACCGTGCGAACGTTGTGGCAGCATCATTGAGAAAACCACGCTGTCATCTCGCCCGTTTTACTGGTGCCCTGGCTGCCAGCACTAGGCCGACCGCTTCGGCGCATAGGTTGaaa                                                                                                           >  1:775202/1‑140 (MQ=255)

                            tAAGGTTTTTCATCGAGATGGCGAACCGTGCGAACGTTGTGGCAGCATCATTGAGAAAACCACGCTGTCATCTCGCCCGTTTTACTGGTGCCCTGGCTGCCAGCACTAGGCCGACCGCTTCGGCGCATAGGTTGAAATaa                                                                                                        >  1:1567574/1‑140 (MQ=255)

                              aGGTTTTTCATCGAGATGGCGAACCGTGCGAACGTTGTGGCAGCATCATTGAGAAAACCACGCTGTCATCTCGCCCGTTTTACTGGTGCCCTGGCTGCCAGCACTAGGCCGACCGCTTCGGCGCATAGGTTGAAATAAAc                                                                                                      >  1:1005465/1‑140 (MQ=255)

                                   tttCATCGAGATGGCGAACCGTGCGAACGTTGTGGCAGCATCATTGAGAAAACCACGCTGTCATCTCGCCCGTTTTACTGGTGCCCTGGCTGCCAGCACTAGGCCGACCGCTTCGGCGCATAGGTTGAAATAAACCgcgc                                                                                                 <  2:8715943/140‑1 (MQ=255)

                                   tttCATCGAGATGGCGAACCGTGCGAACGTTGTGGCAGCATCATTGAGAAAACCACGCTGTCATCTCGCCCGTTTTACTGGTGCCCTGGCTGCCAGCACTAGGCCGACCGCTTCGGCGCATAGGTTGAAATAAACCgcgc                                                                                                 >  2:2490516/1‑140 (MQ=255)

                                        tCGAGATGGCGAACCGTGCGAACGTTGTGGCAGCATCATTGAGAAAACCACGCTGTCATCTCGCCCGTTTTACTGGTGCCCTGGCTGCCAGCACTAGGCCGACCGCTTCGGCGCATAGGTTGAAATAAACCGCGCAATgg                                                                                            <  1:5273255/140‑1 (MQ=255)

                                                gCGAACCGTGCGAACGTTGTGGCAGCATCATTGAGAAAACCACGCTGTCATCTCGCCCGTTTTACTGGTGCCCTGGCTGCCAGCACTAGGCCGACCGCTTCGGCGCATAGGTTGAAATAAACCGCGCAATGGCAGGCCCt                                                                                    <  2:6300370/140‑1 (MQ=255)

                                                  gAACCGTGCGAACGTTGTGGCAGCATCATTGAGAAAACCACGCTGTCATCTCGCCCGTTTTACTGGTGCCCTGGCTGCCAGCACTAGGCCGACCGCTTCGGCGCATAGGTTGAAATAAACCGCGCAATGGCAGGCCCTGt                                                                                  <  2:792549/140‑1 (MQ=255)

                                                     ccGTGCGAACGTTGTGGCAGCATCATTGAGAAAACCACGCTGTCATCTCGCCCGTTTTACTGGTGCCCTGGCTGCCAGCACTAGGCCGACCGCTTCGGCGCATAGGTTGTAATAATCCGCGCAATGGCAGGCCCTGTCAg                                                                               >  2:8310698/1‑140 (MQ=255)

                                                       gTGCGAACGTTGTGGCAGCATCATTGAGAAAACCACGCTGTCATCTCGCCCGTTTTACTGGTGCCCTGGCTGCCAGCACTAGGCCGACCGCTTCGGCGCATAGGTTGAAATAAACCGCGCAATGGCAGGCCCTGTCAGCa                                                                             <  2:3371254/140‑1 (MQ=255)

                                                       gTGCGAACGTTGTGGCAGCATCATTGAGAAAACCACGCTGTCATCTCGCCCGTTTTACTGGTGCCCTGGCTGCCAGCACTAGGCCGACCGCTTCGGCGCATAGGTTGAAATAAACCGCGCAATGGCAGGCCCTGTCAGCa                                                                             <  1:7324641/140‑1 (MQ=255)

                                                       gTGCGAACGTTGTGGCAGCATCATTGAGAAAACCACGCTGTCATCTCGCCCGTTTTACTGGTGCCCTGGCTGCCAGCACTAGGCCGACCGCTTCGGCGCATAGGTTGAAATAAACCGCGCAATGGCAGGCCCTGTCAGCa                                                                             <  1:3154598/140‑1 (MQ=255)

                                                       gTGCGAACGTTGTGGCAGCATCATTGAGAAAACCACGCTGTCATCTCGCCCGTTTTACTGGTGCCCTGGCTGCCAGCACTAGGCCGACCGCTTCGGCGCATAGGTTGAAATAAACCGCGCAATGGCAGGCCCTGTCAGCa                                                                             <  2:2289246/140‑1 (MQ=255)

                                                        tGCGAACGTTGTGGCAGCATCATTGAGAAAACCACGCTGTCATCTCGCCCGTTTTACTGGTGCCCTGGCTGCCAGCTCTAGGCCGACCGCTTCGGCGCATAGGTTGAAATAAACCGCGCAATGGCAGGCCCTGTCAGCaa                                                                            >  2:2769069/1‑140 (MQ=255)

                                                            aaCGTTGTGGCAGCATCATTGAGAAAACCACGCTGTCATCTCGCCCGTTTTACTGGTGCCCTGGCTGCCAGCACTAGGCCGACCGCTTCGGCGCATAGGTTGAAATAAACCGCGCAATGGCAGGCCCTGTCAGCAAAATa                                                                        <  1:3039391/140‑1 (MQ=255)

                                                            aaCGTTGTGGCAGCATCATTGAGAAAACCACGCTGTCATCTCGCCCGTTTTACTGGTGCCCAGGCTGCCAGCACTAGGCCGACCGCTTCGGCGCATAGGATGAAATAAACCGCGCAATGGCAGGCCCTGTCAGCAAAATa                                                                        <  2:216954/140‑1 (MQ=255)

                                                                ttGTGGCAGCATCATTGAGAAAACCACGCTGTCATCTCGCCCGTTTTACTGGTGCCCTGGCTGCCAGCACTAGGCCGACCGCTTCGGCGCATAGGTTGAAATAAACCGCGCAATGGCAGGCCCTGTCAGCAAAATACTGa                                                                    >  2:930204/1‑140 (MQ=255)

                                                                ttGTGGCAGCATCATTGAGAAAACCACGCTGTCATCTCGCCCGTTTTACTGGTGCCCTGGCTGCCAGCACTAGGCCGACCGCTTCGGCGCATAGGTTGAAATAAACCGCGCAATGGCAGGCCCTGTCAGCAAAATACTGa                                                                    >  1:3982436/1‑140 (MQ=255)

                                                                 tgtgGCAGCATCATTGAGAAAACCACGCTGTCATCTCGCCCGTTTTACTTGTGCCCTGGCTGCCAGCACTAGGCCGACCGCTTCGGCGCATAGGTTGAAATAAACCGCGCAATGGCAGGACATGTCAGCAAAATACTGaa                                                                   <  1:493489/140‑1 (MQ=255)

                                                                 tgtgGCAGCATCATTGAGAAAACCACGCTGTCATCTCGCCCGTTTTACTGGTGCCCTGGCTGCCAGCACTAGGCCGACCGCTTCGGCGCATAGGTTGAAATAAACCGCGCAATGGCAGGCCCTGTCAGCAAAATACTGaa                                                                   <  1:6496325/140‑1 (MQ=255)

                                                                 tgtgGCAGCATCATTGAGAAAACCACGCTGTCATCTCGCCCGTTTTACTGGTGCCCTGGCTGCCAGCACTAGGCCGACCGCTTCGGCGCATAGGTTGAAATAAACCGCGCAATGGCAGGCCCTGTCAGCAAAATACTGaa                                                                   <  1:9047526/140‑1 (MQ=255)

                                                                 tgtgGCAGCATCATTGAGAAAACCACGCTGTCATCTCGCCCGTTTTACTGGTGCCCTGGCTGCCAGCACTAGGCCGACCGCTTCGGCGCATAGGTTGAAATAAACCGCGCAATGGCAGGCCCTGTCAGCAAAATACTGaa                                                                   <  1:2379909/140‑1 (MQ=255)

                                                                 tgtgGCAGCATCATTGAGAAAACCACGCTGTCATCTCGCCCGTTTTACTGGTGCCCTGGCTGCCAGCACTAGGCCGACCGCTTCGGCGCATAGGTTGAAATAAACCGCGCAATGGCAGGCCCTGTCAGCAAAATACTGaa                                                                   <  2:8109484/140‑1 (MQ=255)

                                                                 tgtgGCAGCATCATTGAGAAAACCACGCTGTCATCTCGCCCGTTTTACTGGTGCCCTGGCTGCCAGCACTAGGCCGACCGCTTCGGCGCATAGGTTGAAATAAACCGCGCAATGGCAGGCCCTGTCAGCAAAATACTGaa                                                                   <  2:6600454/140‑1 (MQ=255)

                                                                 tgtgGCAGCATCATTGAGAAAACCACGCTGTCATCTCGCCCGTTTTACTGGTGCCCTGGCTGCCAGCACTAGGCCGACCGCTTCGGCGCATAGGTTGAAATAAACCGCGCAATGGCAGGCCCTGTCAGCAAAATACTGaa                                                                   <  1:2497215/140‑1 (MQ=255)

                                                                 tgtgGCAGCATCATTGAGAAAACCACGCTGTCATCTCGCCCGTTTTACTGGTGCCCTGGCTGCCAGCACTAGGCCGACCGCTTCGGCGCATAGGTTGAAATAAACCGCGCAATGGCAGGCCCTGTCAGCAAAATACTGaa                                                                   <  1:4345373/140‑1 (MQ=255)

                                                                 tgtgGCAGCATCATTGAGAAAACCACGCTGTCATCTCGCCCGTTTTACTGGTGCCCTGGCTGCCAGCACTAGGCCGACCGCTTCGGCGCATAGGATGAAATAAACCGCGCAATGGCAGGCCCTGTCAGCAAAATACTGaa                                                                   <  2:8024007/140‑1 (MQ=255)

                                                                 tgtgGCAGCATCATTGAGAAAACCACGCGGACATCGCGCCCGTTTTACTGGTGCCCTGGCTGCCAGCACTAGGCCGACCGCTTCGGCGCATAGGTTGAAATAAACCGCGCAATGGCAGGCCCTGTCAGCAAAATACGGaa                                                                   <  1:6200011/140‑1 (MQ=255)

                                                                   tgGCAGCATCATTGAGAAAACCACGCTGTCATCTCGCCCGTTTTACTGGTGCCCTGGCTGCCAGCACTAGGCCGACCGCTTCGGCGCATAGGTTGAAATAAACCGCGCAATGGCAGGCCCTGTCAGCAAAATACTGAACa                                                                 >  2:1074479/1‑140 (MQ=255)

                                                                        gcaTCATTGAGAAAACCACGCTGTCATCTCGCCCGTTTTACTGGTGCCCTGGCTGCCAGCACTAGGCCGACCGCTTCGGCGCATAGGTTGAAATAAACCGCGCAATGGCAGGCCCTGTCAGCAAAATACTGAACAGGCGt                                                            <  2:5658695/140‑1 (MQ=255)

                                                                        gcaTCATTGAGAAAACCACGCTGTCATCTCGCCCGTTTTACTGGTGCCCTGGCTGCCAGCACTAGGCCGACCGCTTCGGCGCATAGGTTGAAATAAACCGCGCAATGGCAGGCCCTGTCAGCAAAATACTGAACAGGCGt                                                            <  1:8054947/140‑1 (MQ=255)

                                                                        gcaTCATGGCGAACACCACGCTGTCATCTCGCCCGTTCTACTGGTGCCCTGGCTGCCAGCACAAGGCCGACCGCTTCGGCGCATAGGTTGAAATACACCGCGCAATGGCAGGCCCTGTCAGCAAAATACTGAACAGGCGt                                                            <  1:2835571/140‑1 (MQ=255)

                                                                           tcatTGAGAAAACCACGCTGTCATCTCGCCCGTTTTACTGGTGCCCTGGCTGCCAGCACTAGGCCGACCGCTTCGGCGCATAGGTTGAAATAAACCGCGCAATGGCAGGCCCTGTCAGCAAAATACTGaa                                                                   <  2:9013176/130‑1 (MQ=255)

                                                                                  gaAAACCACGCTGTCATCTCGCCCGTTTTACTGGTGCCCTGGCTGCCAGCACTAGGCCGACCGCTTCGGCGCATAGGTTGAAATAAACCGCGCAATGGCAGGCCCTGTCAGCAAAATACTGAACAGGCGTAGGGTTTGCa                                                  <  2:4490224/140‑1 (MQ=255)

                                                                                  gaAAACCACGCTGTCATCTCGCCCGTTTTACTGGTGCCCTGGCTGCCAGCACTAGGCCGACCGCTTCGGCGCATAGGTTGAAATAAACCGCGCAATGGCAGGCCCTGTCAGCAAAATACTGAACAGGCGTAGGGTTTGCa                                                  <  2:9386706/140‑1 (MQ=255)

                                                                                  gaAAACCACGCTGTCATCTCGCCCGTTTTACTGGTGCCCTGGCTGCCAGCACTAGGCCGACCGCTTCGGCGCATAGGTTGAAATAAACCGCGCAATGGCAGGCCCTGTCAGCAAAATACTGAACAGGCGTAGGGTTTGCa                                                  <  1:4080332/140‑1 (MQ=255)

                                                                                  gaAAACCACGCTGTCATCTCGCCCGTTTTACTGGTGCCCTGGCTGCCAGCACTAGGCCGACCGCTTCGGCGCATAGGTTGAAATAAACCGCGCAATGGCAGGCCCTGTCAGCAAAATACTGAACAGGCGTAGGGTTTGCa                                                  <  1:8516050/140‑1 (MQ=255)

                                                                                  gaAAACCACGCTGTCATCTCGCCCGTTTTACTGGTGCCCTGGCTGCCAGCACTAGGCCGACCGCTTCGGCGCATAGGTTGAAATAAACCGCGCAATGGCAGGCCCTGTCAGCAAAATACTGAACAGGCGTAGGGTTTGCa                                                  <  1:6339303/140‑1 (MQ=255)

                                                                                  gaAAACCACGCTGTCATCTCGCCCGTTTTACTGGTGCCCGGGCTGCCAGCACTAGGCCGACCGCTTCGGCGCATAGGTTGAAATAAACCGCGCAATGGCAGGCCCTGTCAGCAAAATACTGAACAGGCGTAGGGTTTGCa                                                  <  1:7259093/140‑1 (MQ=255)

                                                                                   aaaaCCACGCTGTCATCTCGCCCGTTTTACTGGTGCCCTGGCTGCCAGCACTAGGCCGACCGCTTCGGCGCATAGGTTGAAATAAACCGCGCAATGGCAGGCCCTGTCAGCAAAATACTGAACAGGCGTAGGGTTTGCAt                                                 >  1:141700/1‑140 (MQ=255)

                                                                                   aaaaCCACGCTGTCATCTCGCCCGTTTTACTGGTGCCCTGGCTGCCAGCACTAGGCCGACCGCTTCGGCGCATAGGTTGAAATAAACCGCGCAATGGCAGGCCCTGTCAGCAAAATACTGAACAGGCGTAGGGTTTGCAt                                                 >  1:4687600/1‑140 (MQ=255)

                                                                                           gCTGTCATCTCGCCCGTTTTACTGGTGCCCTGGCTGCCAGCACTAGGCCGACCGCTTCGGCGCATAGGTTGAAATAAACCGCGCAATGGCAGGCCCTGTCAGCAAAATACTGAACAGGCGGAGGGTTTGCATCGCCATaa                                         >  1:3817189/1‑140 (MQ=255)

                                                                                             tGTCATCTCGCCCGTTTTACTGGTGCCCTGGCTGCCAGCACTAGGCCGACCGCTTCGGCGCATAGGTTGAAATAAACCGCGCAATGGCAGGCCCTGTCAGCAAAATACTGAACAGGCGTAGGGTTTGCATCGCCATAATg                                       >  1:6989445/1‑140 (MQ=255)

                                                                                               tCATCTCGCCCGTTTTACTGGTGCCCTGGCTGCCAGCACTAGGCCGACCGCTTCGGCGCATAGGTTGAAATAAACCGCGCAATGGCAGGCCCTGTCAGCAAAATACTGAACAGGCGTAGGGTTTGCATCGCCATAATGAg                                     <  2:3783112/140‑1 (MQ=255)

                                                                                               tCATCTCGCCCGTTTTACTGGTGCCCTGGCTGCCAGCACTAGGCCGACCGCTTCGGCGCATAGGTTGAAATAAACCGCGCAATGGCAGGCCCTGTCAGCAAAATACTGAACAGGCGTAGGGTTTGCATCGCCATAATGAg                                     <  1:7901736/140‑1 (MQ=255)

                                                                                               tCATCTCGCCCGTTTTACTGGTGCCCTGGCTGCCAGCACTAGGCCGACCGCTTCGGCGCATAGGTTGAAATAAACCGCGCAATGGCAGGCCCTGTCAGCAAAATACTGAACAGGCGTAGGGTTTGCATCGCCATAATGAg                                     <  1:45811/140‑1 (MQ=255)

                                                                                               tCATCTCGCCCGTTTGACAGGTGCCCTGCCTGCCAGCACGAGGCCGACCGCTTCGGCGCATAGGTGGAAATAAACCGCGCAATGGCAGGCCCTGTCAGCAAAATACTGAACAGGCGTAGGGTTTGCATCGCCATAATGAg                                     <  2:6825504/140‑1 (MQ=255)

                                                                                                cATCTCGCCCGTTTTACTGGTGCCCTGGCTGCCAGCACTAGGCCGACCGCTTCGGCGCATAGGTTGAAATAAACCGCGCAATGGCAGGCCCTGTCAGCAAAATACTGAACAGGCGTAGGGTTTGCATCGCCATAATGAgc                                    <  1:8143509/140‑1 (MQ=255)

                                                                                                cATCTCGCCCGTTTTACTGGTGCCCTGGCTGCCAGCACTAGGCCGACCGCTTCGGCGCATAGGTTGAAATAAACCGCGCAATGGCAGGCCCTGTCAGCAAAATACTGAACAGGCGTAGGGTTTGCATCGCCATAATGAgc                                    >  2:5458833/1‑140 (MQ=255)

                                                                                                cATCTCGCCCGTTTTACTGGTGCCCTGGCTGCCAGCACTAGGCCGACCGCTTCGGCGCATAGGTTGAAATAAACCGCGCAATGGCAGGCCCTGTCAGCAAAATACTGAACAGGCGTAGGGTTTGCATCGCCATAATGAgc                                    <  2:8907198/140‑1 (MQ=255)

                                                                                                       cccGTTTTACTGGTGCCCTGGCTGCCAGCACTAGGCCGACCGCTTCGGCGCATAGGTTGAAATAAACCGCGCACTGGCAGGCCCTGTCAGCAAAATACGGAACAGGCGGAGGGTTTGCATCGCCATAATGAGCGCCctat                             >  2:3131202/1‑140 (MQ=255)

                                                                                                       cccGTTTTACTGGTGCCCTGGCTGCCAGCACTAGGCCGACCGCTTCGGCGCATAGGTTGAAATAAACCGCGCAATGGCAGGCCCTGTCAGCAAAATACTGAACAGGCGTAGGGTTTGCATCGCCATAATGAgcgc                                  >  1:1763026/1‑135 (MQ=255)

                                                                                                       cccGTTTTACTGGTGCCCTGGCTGCCAGCACTAGGCCGACCGCTTCGGCGCATAGGTTGAAATAAACCGCGCAATGGCAGGCCCTGTCAGCAAAATACTGAACAGGCGTAGGGTTTGCATCGCCATAATGAgcgc                                  <  2:1763026/135‑1 (MQ=255)

                                                                                                       cccGTTTTACTGGTGCCCTGGCTGCCAGCACTAGGCCGACCGCTTCGGCGCATAGGTTGAAATAAACCGCGCAATGGCAGGCCCTGTCAGCAAAATACTGAACAGGCGTAGGGTTTGCATCGCCATAATGAGCGCCatat                             >  1:5147378/1‑140 (MQ=255)

                                                                                                       cccGTTTTACTGGTGCCCTGGCTGCCAGCACTAGGCCGACCGCTTCGGCGCATAGGTTGAAATAAACCGCGCAATGGCAGGCCCTGTCAGCAAAATACTGAACAGGCGTAGGGTTTGCATCGCCATAATGAGCGCCatat                             <  1:2766353/140‑1 (MQ=255)

                                                                                                       cccGTTTTACTGGTGCCCTGGCTGCCAGCACTAGGCCGACCGCTTCGGCGCATAGGTTGAAATAAACCGCGCAATGGCAGGCCCTGTCAGCAAAATACTGAACAGGCGTAGGGTTTGCATCGCCATAATGAGCGCCatat                             <  2:1600910/140‑1 (MQ=255)

                                                                                                               aCTGGTGCCCAGGCGGCCAGCCCTCGGCCGCCCGCGTCGGCGCAGCGGTTGAAATAAACCGCGCCATGGCAGGCCCTGTCAGCAAAATACTGAACAGGCGTAGGGTGTGCATCGCCATAATGAGCGCCATATCGGCATTg                     <  2:7272643/140‑1 (MQ=255)

                                                                                                                 tGGTGCCCTGGCTGCCAGCACTAGGCCGACCGCTTCGGCGCATAGCTTGAAAAAAACCGCGCAATGGCAGGCCCTGTCAGCAAAATACTGAACAGGCGTAGTGTTTGCATCGCCATAATGAGCGCCATATCGGCATTGCt                   >  2:7676086/1‑140 (MQ=255)

                                                                                                                  ggTGCCCTGGCTGCCAGCACTAGGCCGACCGCTTCGGCGCATAGGTTGAAATAAACCGCGCAATGGCAGGCCCTGTCAGCAAAATACTGAACAGGCGTAGGGTTTGCATCGCCATAATGAGCGCCATATCGGCATTGCTc                  >  1:7602918/1‑140 (MQ=255)

                                                                                                                     gCCCTGGCTGCCAGCACTAGGCCGACCGCTTCGGCGCATAGGTTGAAATAAACCGCGCAATGGCAGGCCCTGTCAGCAAAATACTGAACAGGCGTAGGGTTTGCATCGCCATAATGAGCGCCATATCGGCATTGCTCCCt               >  1:942516/1‑140 (MQ=255)

                                                                                                                     gCCCTGGCTGCCAGCACTAGGCCGACCGCTTCGGCGCATAGGTTGAAATAAACCGCGCAATGGCAGGCCCTGTCAGCAAAATACTGAACAGGCGTAGGGTTTGCATCGCCATAATGAGCGCCATATCGGCATTGCTCCCt               >  1:8335549/1‑140 (MQ=255)

                                                                                                                     gCCCTGGCTGCCAGCACTAGGCCGACCGCTTCGGCGCATAGGTTGAAATAAACCGCGCAATGGCAGGCCCTGTCAGCAAAATACTGAACAGGCGTAGGGTTTGCATCGCCATAATGAGCGCCATATCGGCATTGCTCCCt               >  1:4490663/1‑140 (MQ=255)

                                                                                                                      cccTGGCTGCCAGCACTAGGCCGACCGCTTCGGCGCATAGGTTGAAATAAACCGCGCAATGGCAGGCCCTGTCAGCAAAATACTGAACAGGCGTAGGGTTTGCATCGCCATAATGAGCGCCATATCGGCATTGCTCCCTg              >  2:5332912/1‑140 (MQ=255)

                                                                                                                      cccTGGCTGCCAGCACTAGGCCGACCGCTTCGGCGCATAGGTTGAAATAAACCGCGCAATGGCAGGCCCTGTCAGCAAAATACTGAACAGGCGTAGGGTTTGCATCGCCATAATGAGCGCCATATCGGCATTGCTCCCTg              >  2:4277917/1‑140 (MQ=255)

                                                                                                                      cccTGGCTGCCAGCACTAGGCCGACCGCTTCGGCGCATAGGTTGAAATAAACCGCGCAATGGCAGGCCCTGTCAGCAAAATACTGAACAGGCGTAGGGTTTGCATCGCCATAATGAGCGCCATATCGGCATTGCTCCCTg              >  2:3616962/1‑140 (MQ=255)

                                                                                                                      cccTGGCTGCCAGCACTAGGCCGACCGCTTCGGCGCATAGGTTGAAATAAACCGCGCAATGGCAGGCCCTGTCAGCAAAATACTGAACAGGCGTAGGGTTTGCATCGCCATAATGAGCGCCATATCGGCATTGCTCCCTg              >  1:1215284/1‑140 (MQ=255)

                                                                                                                           gCTGCCAGCACTAGGCCGACCGCGTCGGCGCATAGGTTGAAATAAACCGCGCAATGGCAGGCCCTGTCAGCAAAATACTGAACAGGCGTAGGGTTTGCATCGCCATAATGAGCGCCATATCGGCATTGCTCCCTgcggcg         <  2:4356119/140‑1 (MQ=255)

                                                                                                                             tGCCAGCACTAGGCCGACCGCTTCGGCGCATAGGTTGAAATAAACCGCGCAATGGCAGGCCCTGTCAGCAAAATACTGAACAGGCGTAGGGTTTGCATCGCCATAATGAGCGCCATATCGGCATTGCTCCCTGCGGCGAt       <  1:6120112/140‑1 (MQ=255)

                                                                                                                              gCCAGCACTAGGCCGACCGCTTCGGCGCATAGGTTGAAATAAACCGCGCAATGGCAGGCCCTGTCAGCAAAATACTGAACAGGCGTAGGGTTTGCATCGCCATAATGAGCGCCATATCGGCATTGCTCCCTGCGGCGATg      >  1:2089407/1‑140 (MQ=255)

                                                                                                                               ccAGCACTAGGCCGACCGCTTCGGCGCATAGGTTGAAATAAACCGCGCAATGGCAGGCCCTGTCAGCAAAATACTGAACAGGCGTAGGGTTTGCATCGCCATAATGAGCGCCATATCGGCATTGCTCCCTGCGGCGATGa     >  2:3984240/1‑140 (MQ=255)

                                                                                                                                cAGCACTAGGCCGACCGCTTCGGCGCNTAGGTTGAAATAAACCGCGCAATGGCAGGCCCTGTCAGCAAAATACTGAACAGGCGTAGGGTTTGCATCGCCATAATGAGCGCCATATCGGCATTGCTCNCTGCGGCGATGAc    <  1:5534192/140‑1 (MQ=255)

                                                                                                                                cAGCACTAGGCCGACCGCTTCGGCGCATAGGTTGAAATAAACCGCGCAATGGCAGGCCCTGTCAGCAAAATACTGAACAGGCGTAGGGTTTGCATCGCCATAATGAGCGCCATATCGGCATTGCTCCCTGCGGCGATGAc    <  2:199447/140‑1 (MQ=255)

ATAAGCATCATGGGGCGCTGTTTCGCTTTAAGGTTTTTCATCGAGATGGCGAACCGTGCGAACGTTGTGGCAGCATCATTGAGAAAACCACGCTGTCATCTCGCCCGTTTTACTGGTGCCCTGGCTGCCAGCATTAAGCCGACCGCTTCGGCGCATAGGTTGAAATAAACCGCGCAATGGCAGGCCCTGTCAGCAAAATACTGAACAGGCGTAGGGTTTGCATCGCCATAATGAGCGCCATATCGGCATTGCTCCCTGCGGCGATGACCG  >  NZ_CP025268/745639‑745908

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`