BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP025268	801,562	C→A	L54I (CTT→ATT)	ybhJ →	hydratase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP025268	801,562	0	C	A	99.3%	1709.9 / NA	451	L54I (CTT→ATT)	ybhJ	hydratase
Reads supporting (aligned to +/- strand): ref base C (1/2); new base A (153/295); total (154/297)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 457 total aligned reads displayed.

GCGTGTTTCTCGCCAGTAATAACGAAATAATTGCCGAAGAACATTTCACCGGCGAAATTAAAAAAGAAGAAGCCAAAAAAGGCACTATTGCCTGGTCTATTCTCTCTTCGCATAATACGTCCGGAAATATGGATAAACTTAAAATTAAGTTTGATTCATTAGCCTCTCACGATATTACCTTTGTTGGTATTGTACAGACCGCTAAAGCGTCCGGTATGGAACGTTT‑CCCGCTGCCGTATGTGCTGACCAACTGCCATAACTCACTCTGCGCCGTCGG  >  NZ_CP025268/801425‑801701

gCGTGTTTCTCGCCAGTAATAACGAAATAATTGCCGAAGAACATTTCACCGGCGAAATTAAAAAAGAAGAAGCCAAAAAAGGCACTATTGCCTGGTCTATTCTCTCTTCGCATAATACGTCCGGAAATATGGATAAAAtt                                                                                                                                            >  1:1665432/1‑140 (MQ=255)

gCGTGTTTCTCGCCAGTAATAACGAAATAATTGCCGAAGAACATTTCACCGGCGAAATTAAAAAAGAAGAAGCCAAAAAAGGCACTATTGCCTGGTCTATTCTCTCTTCGCATAATACGTCCGGAAATATGGATAAAAtt                                                                                                                                            <  2:3800199/140‑1 (MQ=255)

 cGTGTTTCTCGCCAGTAATAACGAAATANTTGCCGAAGAACATTTCACCGGCGAAATTAAAAAAGAAGAAGCCAAAAAAGGCACTATTGCCTGGTCTATTCTCTCTTCGCATAATACGTCCGGAAATATGGATAAAATTa                                                                                                                                           <  1:5553377/140‑1 (MQ=255)

    gtTTCTCGCCAGTAATAACGAAATAATTGCCGAAGAACATTTCACCGGCGAAATTAAAAAAGAAGAAGCCAAAAAAGGCACTATTGCCTGGTCTATTCTCTCTTCGCATAATACGTCCGGAAATATGGATAAAATTaaaa                                                                                                                                        >  2:4465623/1‑140 (MQ=255)

      ttCTCGCCAGTAATAACGAAATAATTGCCGAAGAACATTTCACCGGCGAAATTAAAAAAGAAGAAGCCAAAAAAGGCACTATTGCCTGGTCTATTCTCTCTTCGCATAATACGTCCGGAAATATGGATaaaattaaaatt                                                                                                                                      <  1:7323931/140‑1 (MQ=255)

      ttCTCGCCAGTAATAACGAAATAATTGCCGAAGAACATTTCACCGGAGAAATTAAAAAAGAAGAAGCCAAAAAAGGCACTATTGCCTGGTCTATTCTCTCTTCGCATAATACGTCCGGAAATATGGATaaaattaaaatt                                                                                                                                      <  1:5166290/140‑1 (MQ=255)

             cAGTAATAACGAAATAATTGCCGAAGAACATTTCACCGGCGAAATTAAAAAAGAAGAAGCCAAAAAAGGCACTATTGCCTGGTCTATTCTCTCTTCGCATAATACGTCCGGAAATATGGATAAAATTAAAATTAAGTTTg                                                                                                                               <  1:4682592/140‑1 (MQ=255)

             cAGTAATAACGAAATAATTGCCGAAGAACATTTCACCGGCGAAATTAAAAAAGAAGAAGCCAAAAAAGGCACTATTGCCTGGTCTATTCTCTCTTCGCATAATACGTCCGGAAATATGGATAAAATTAAAATTAAGTTTg                                                                                                                               <  1:587513/140‑1 (MQ=255)

              aGTAATAACGAAATAATTGCCGAAGAACATTTCACCGGCGAAATTAAAAAAGAAGAAGCCAAAAAAGGCACTATTGCCTGGTCTATTCTCTCTTCGCATAATACGTCCGGAAATATGGATAAAATTAAAATTAAGTTTGa                                                                                                                              <  1:2009229/140‑1 (MQ=255)

                  ataaCGAAATAATTGCCGAAGAACATTTCACCGGCGAAATTAAAAAAGAAGAAGCCAAAAAAGGCACTATTGCCTGGTCTATTCTCTCTTCGCATAATACGTCCGGAAATATGGATAAAATTAAAATTAAGTTTGATTCa                                                                                                                          <  2:9285263/140‑1 (MQ=255)

                   taaCGAAATAATTGCCGAAGAACATTTCACCGGCGAAATTAAAAAAGAAGAAGCCAAAAAAGGCACTATTGCCTGGTCTATTCTCTCTTCGCATAATACGTCCGGAAATATGGATAAAATTAAAATTAAGTTTGATTCAt                                                                                                                         <  1:7984957/140‑1 (MQ=255)

                    aaCGAAATAATTGCCGAAGAACATTTCACCGGCGAAATTAAAAAAGAAGAAGCCAAAAAAGGCACTATTGCCTGGTCTATTCTCTCTTCGCATAATACGTCCGGAAATATGGATAAAATTAAAATTAAGTTTGATTCAtt                                                                                                                        <  1:1344224/140‑1 (MQ=255)

                      cGAAATAATTGCCGAAGAACATTTCACCGGCGAAATTAAAAAAGAAGAAGCCAAAAAAGGCACTATTGCCTGGTCTATTCTCTCTTCGCATAATACGTCCGGAAATATGGATAAAATTAAAATTAAGTTTGATTCATTAg                                                                                                                      <  2:7209988/140‑1 (MQ=255)

                       gAAATAATTGCCGAAGAACATTTCACCGGCGAAATTAAAAAAGAAGAAGCCAAAAAAGGCACTATTGCCTGGTCTATTCTCTCTTCGCATAATACGTCCGGAAATATGGATAAAATTAAAATTAAGTTTGATTCATTAGc                                                                                                                     <  2:4266326/140‑1 (MQ=255)

                          ataatTGCCGAAGAACATTTCACCGTCGAAATTAAAAAAGAAGAAGCCAAAAAAGGCACTATTGCCTGGTCTATTCTCTCTTCGCATAATAAGTCCTGAAATATGGATAAAATTAAAATTAAGTTTTATTCATTAGCctc                                                                                                                  >  2:2481870/1‑140 (MQ=255)

                           taatTGCCGAAGAACATTTCACCGGCGAAATTAAAAAAGAAGAAGCCAAAAAAGGCACTATTGCCTGGTCTATTCTCTCTTCGCATAATACGTCCGGAAATATGGATAAAATTAAAATTAAGTTTGATTCATTAGCctct                                                                                                                 <  1:6393911/140‑1 (MQ=255)

                           taagTGCCGAAGAACATTTCACCGGCGAAATTAAAAAAGAAGAAGCCAAAAAAGGCACTATTGCCTGGTCTATTCTCTCTTCGCATAATACGTCCGGAAATATGGATAAAATTAAAATTAAGTTTGATTCATTAGCctct                                                                                                                 <  2:3031849/140‑1 (MQ=255)

                            aatTGCCGAAGAACATTTCACCGGCGAAATTAAAAAAGAAGAAGCCAAAAAAGGCACTATTGCCTGGTCTATTCTCTCTTCGCATAATACGTCCGGAAATATGGATAAAATTAAAATTAAGTTTGATTCATTAGCCtctc                                                                                                                <  2:4704672/140‑1 (MQ=255)

                            aatTGCCGAAGAACATTTCACCGGCGAAATTAAAAAAGAAGAAGCCAAAAAAGGCACTATTGCCTGGTCTATTCTCTCTTCGCATAATACGTCCGGAAATATGGATAAAATTAAAATTAAGTTTGATTCATTAGCCtctc                                                                                                                <  2:809787/140‑1 (MQ=255)

                             atTGCCGAAGAACATTTCACCGGCGAAATTAAAAAAGAAGAAGCCAAAAAAGGCACTATTGCCTGGTCTATTCTCTCTTCGCATAATACGTCCGGAAATATGGATAAAATTAAAATTAAGTTTGATTCATTAGCCTCTCa                                                                                                               <  2:1472313/140‑1 (MQ=255)

                             atTGCCGAAGAACATTTCACCGGCGAAATTAAAAAAGAAGAAGCCAAAAAAGGCACTATTGCCTGGTCTATTCTCTCTTCGCATAATACGTCCGGAAATATGGATAAAATTAAAATTAAGTTTGATTCATTAGCCTCTCa                                                                                                               <  2:138082/140‑1 (MQ=255)

                              ttGCCGAAGAACATTTCACCGGCGAAATTAAAAAAGAAGAAGCCAAAAAAGGCACTATTGCCTGGTCTATTCTCTCTTCGCATAATACGTCCGGAAATATGGATAAAATTAAAATTAAGTTTGATTCATTAGCCTCTCAc                                                                                                              >  1:3311928/1‑140 (MQ=255)

                                gCCGAAGAACATTTCACCGGCGAAATTAAAAAAGAAGAAGCCAAAAAAGGCACTATTGCCTGGTCTATTCTCTCTTCGCATAATACGTCCGGAAATATGGATAAAATTAAAATTAAGTTTGATTCATTAGCCTCTCACGa                                                                                                            <  1:43556/140‑1 (MQ=255)

                                 ccGAAGAACATTTCACCGGCGAATTTAAAAAAGAAGAAGCCAAAAAAGGCACTATTGCCTGGTCTATTCTCTCTTCGCATAATACGTCCGGAAATATGGATAAAATTAAAATTAAGTTTGATTCATTAGCCTCTCACGat                                                                                                           >  1:2354869/1‑140 (MQ=255)

                                  cGAAGAACATTTCACCGGCGAAATTAAAAAAGAAGACGCCAAAAAAGGCACTATTGCCTGGTCTATTCTCTCTTCGCATAATACGTCCGGAAATATGGATAAAATTAAAATTAAGTTTGATTCATTAGCCTCTCACGata                                                                                                          <  2:5558144/140‑1 (MQ=255)

                                   gaagaaCATTTCACCGGCGAAATGAAACAAGAAGAAGCCAAAAAAGGCACTATTGCCTGGTCTATTCTCTCTTCGCATAATACGTCCGGAAATATGGATAAAATTAAAATTAAGTTTGATTCATTAGCCTCTCACGatat                                                                                                         <  1:5640566/140‑1 (MQ=255)

                                    aagaaCATTTCACCGGCGAAATTAAAAAAGAAGAAGCCAAAAAAGGCACTATTGCCTGGTCTATTCTCTCTTCGCATAATACGTCCGGAAATATGGATAAAATTAAAATTAAGTTTGATTCATTAGCCTCTCACGATAtt                                                                                                        <  1:7184342/140‑1 (MQ=255)

                                      gaaCATTTCACCGGCGAAATTAAAAAAGAAGAAGCCAAAAAAGGCACTATTGCCTGGTCTATTCTCTCTTCGCATAATACGTCCGGAAATATGGATAAAATTAAAATTAAGTTTGATTCATTAGCCTCTCACGATATTAc                                                                                                      <  1:3985512/140‑1 (MQ=255)

                                       aaCATTTCACCTGCGAAATTAAAAAAGAAGAAGCCAAAAAAGGCACTATTGCCTGGTCTATTCTCTCTTCGCATAATACGTCCGGAAATATGGATAAAATTAAAATTAAGTTTGATTCATTAGCCTCTCACGATATTAcc                                                                                                     <  2:6756506/140‑1 (MQ=255)

                                       aaCATTTCACCGGCGAAATTAAAAAAGAAGAAGCCAAAAAAGGCACTATTGCCTGGTCTATTCTCTCTTCGCATAATACGTCCGGAAATATGGATAAAATTAAAATTAAGTTTGATTCATTAGCCTCTCACGATATTAcc                                                                                                     <  1:3116222/140‑1 (MQ=255)

                                            ttCACCGGCGAAATTAAAAAAGAAGAAGCCAAAAAAGGCACTATTGCCTGGTCTATTCTCTCTTCGCATAATACGTCCGGAAATATGGATAAAATTAAAATTAAGTTTGATTCATTAGCCTCTCACGATATTACCTttgt                                                                                                <  2:4939459/140‑1 (MQ=255)

                                            ttCACCGGCGAAATTAAAAAAGAAGAAGCCAAAAAAGGCACTATTGCCTGGTCTATTCTCTCTTCGCATAATACGTCCGGAAATATGGATAAAATTAAAATTAAGTTTGATTCATTAGCCTCTCACGATATTACCTttgt                                                                                                <  1:8923771/140‑1 (MQ=255)

                                            ttCACCGGCGAAATTAAAAAAGAAGAAGCCAAAAAAGGCACTATTGCCTGGTCTATTCTCTCTTCGCATAATACGTCCGGAAATATGGATAAAATTAAAATTAAGTTTGATTCATTAGCCTCTCACGATATTACCTttgt                                                                                                <  2:2738458/140‑1 (MQ=255)

                                            ttCACCGGCGAAATTAAAAAAGAAGAAGCCAAAAAAGGCACTATTGCCTGGTCTATTCTCTCTTCGCATAATACGTCCGGAAATATGGATAAAATTAAAATTAAGTTTGATTCATTAGCCTCTCACGATATTACCTttgt                                                                                                <  2:3254303/140‑1 (MQ=255)

                                            ttCACCGGCGAAATTAAAAAAGAAGAAGCCAAAAAAGGCACTATTGCCTGGTCTATTCTCTCTTCGCATAATACGTCCGGAAATATGGATAAAATTAAAATTAAGTTTGATTCATTAGCCTCTCACGATATTACCTttgt                                                                                                <  2:3458510/140‑1 (MQ=255)

                                            ttCACCGGCGAAATTAAAAAAGAAGAAGCCAAAAAAGGCACTATTGCCTGGTCTATTCTCTCTTCGCATAATACGTCCGGAAATATGGATAAAATTAAAATTAAGTTTGATTCATTAGCCTCTCACGATATTACCTttgt                                                                                                <  1:3744681/140‑1 (MQ=255)

                                            ttCACCGGCGAAATTAAAAAAGAAGAAGCCAAAAAAGGCACTATTGCCTGGTCTATTCTCTCTTCGCATAATACGTCCGGAAATATGGATAAAATTAAAATTAAGTTTGATTCATTAGCCTCTCACGATATTACCTttgt                                                                                                <  1:4137158/140‑1 (MQ=255)

                                            ttCACCGGCGAAATTAAAAAAGAAGAAGCCAAAAAAGGCACTATTGCCTGGTCTATTCTCTCTTCGCATAATACGTCCGGAAATATGGATAAAATTAAAATTAAGTTTGATTCATTAGCCTCTCACGATATTACCTttgt                                                                                                <  2:9099385/140‑1 (MQ=255)

                                              cACCGGCGAAATTAAAAAAGAAGAAGCCAAAAAAGGCACTATTGCCTGGTCTATTCTCTCTTCGCATAATACGTCCGGAAATATGGATAAAATTAAAATTAAGTTTGATTCATTAGCCTCTCACGATATTACCTttgttg                                                                                              <  1:815818/140‑1 (MQ=255)

                                                 cGGCGAAATTAAAAAAGAAGAAGCCAAAAAAGGCACTATTGCCTGGTCTATTCTCTCTTCGCATAATACGTCCGGAAATATGGATAAAATTAAAATTAAGTTTGATTCATTAGCCTCTCACGATATTACCTTTGTTGGTa                                                                                           <  1:2702012/140‑1 (MQ=255)

                                                 cGGCGAAATTAAAAAAGAAGAAGCCAAAAAAGGCACTATTGCCTGGTCTATTCTCTCTTCGCATAATACGTCCGGAAATATGGATAAAATTAAAATTAAGTTTGATTCATTAGCCTCTCACGATATTACCTTTGTTGGTa                                                                                           <  2:5102994/140‑1 (MQ=255)

                                                 cGGCGAAATTAAAAAAGAAGAAGCCAAAAAAGGCACTATTGCCTGGTCTATTCTCTCTTCGCATAATACGTCCGGAAATATGGATAAAATTAAAATTAAGTTTGATTCATTAGCCTCTCACGATATTACCTTTGTTGGTa                                                                                           <  2:1150989/140‑1 (MQ=255)

                                                 cGGCGAAATTAAAAAAGAAGAAGCCAAAAAAGGCACTATTGCCTGGTCTATTCTCTCTTCGCATAATACGTCCGGAAATATGGATAAAATTAAAATTAAGATTGATTCATTAGCCTCTCACGATATTACCTTTGTTGGTa                                                                                           <  1:7000253/140‑1 (MQ=255)

                                                 cGGAGAAATTAAAAAAGAAGAAGCCAAAAAAGTCACTATTGCCTGGTCTATTCTCTATTCGCATAATACGTCCGGAAATATGGATAAAATTAAAATTAAGTTTGATTCATTAGCCTCTCACGATATTACCTTTGTTGGTa                                                                                           <  2:5433551/140‑1 (MQ=255)

                                                    cGAAATTAAAAAAGAAGAAGCCAAAAAAGGCACTATTGCCTGGTCTATTCTCTCTTCGCATAATACGTCCGGAAATATGGATAAAATTAAAATTAAGTTTGATTCATTAGCCTCTCACGATATTACCTTTGTTGGTATTg                                                                                        >  1:4884899/1‑140 (MQ=255)

                                                        aTTAAAAAAGAAGAAGCCAAAACAGGCACTATTGCCTGGTCTATTCTCTCTTCGCATAATACGTCCGGAAATATGGATAAAATTAAAATTAAGTTTGATTCATTAGCCTCTCACGATATTACCTTTGTTGGTATTGTACa                                                                                    <  1:8508061/140‑1 (MQ=255)

                                                        aTTAAAAAAGAAGAAGCCAAAAAAGGCACTATTGCCTGGTCTATTCTCTCTTCGCATAATACGTCCGGAAATATGGATAAAATTAAAATTAAGTTTGATTCATTAGCCTCTCACGATATTACCTTTGTTGGTATTGTACa                                                                                    <  1:671110/140‑1 (MQ=255)

                                                        aTTAAAAAAGAAGAAGCCAAAAAAGGCACTATTGCCTGGTCTATTCTCTCTTCGCATAATACGTCCGGAAATATGGATAAAATTAAAATTAAGTTTGATTCATTAGCCTCTCACGATATTACCTTTGTTGGTATTGTACa                                                                                    <  2:2241472/140‑1 (MQ=255)

                                                                 gaagaagCCAAAAAAGGCACTATTGCCTGGTCTATTCTCTCTTCGCATAATACGTCCGGAAATATGGATAAAATTAAAATTAAGTTTGATTCATTAGCCTCTCACGATATTACCTTTGTTGGTATTGTACAGACCGCTaa                                                                           <  1:305370/140‑1 (MQ=255)

                                                                   agaagCCAAAAAAGGCACTATTGCCTGGTCTATTCTCTCTTCGCATAATACGTCCGGAAATATGGATAAAATTAAAATTAAGTTTGATTCATTAGCCTCTCACGATATTACCTTTGTTGGTATTGTACAGACCGCTAAAg                                                                         >  1:8645036/1‑140 (MQ=255)

                                                                       gCCAAAAAAGGCACTATTGCCTGGTCTATTCTCTCTTCGCATAATACGTCCGGAAATATGGATAAAATTAAAATTAAGTTTGATTCATTAGCCTCTCACGATATTACCTTTGTTGGTATTGTACAGACCGCTAAAGCGTc                                                                     <  1:4483683/140‑1 (MQ=255)

                                                                            aaaaGGCACTATTGCCTGGTCTATTCTCTCTTCGCATAATACGTCCGGAAATATGGATAAAATTAAAATTAAGTTTGATTCATTAGCCTCTCACGATATTACCTTTGTTGGTATTGTACAGACCGCTAAAGCGTCCGGTa                                                                >  2:8194361/1‑140 (MQ=255)

                                                                             aaaGGCACTATTGCCTGGTCTATTCTCTCTTCGCATAATACGTCCGGAAATATGGATAAAATTAAAATTAAGTTTGATTCATTAGCCTCTCACGATATTACCTTTGTTGGTATTGTACAGACCGCTAAAGCGTCCGGTAt                                                               <  2:8783817/140‑1 (MQ=255)

                                                                                 gCACTATTGCCTTGTCTATTCTCTCTTCGCATAATACGGCCGGAAATATGGATAAAATTAAAATTAAGTTTGATTCATTAGCCTCTCACGATATTACCTTTGTTGGTATTGTACAGACCGCTAAAGCGTCCGGTATGGaa                                                           <  2:6394539/140‑1 (MQ=255)

                                                                                  cACTATTGCCTGGTCTATTCTCTCTTCGCATAATACGTCCGGAAATATGGATAAAATTAAAATTAAGTTTGATTCATTAGCCTCTCACGATATTACCGTTGTTGGTATTGTACAGACCGCTAAAGCGTCCGGTATGGAAc                                                          >  2:1290716/1‑140 (MQ=255)

                                                                                   aCTATTGCCTGGTCTATTCTCTCTTCGCATAATACGTCCGGAAATATGGATAAAATTAAAATTAAGTTTGATTCATTAGCCTCTCACGATATTACCTTTGTTGGTATTGTACAGACCGCTAAAGCGTCCGGTATGGAACg                                                         <  2:465657/140‑1 (MQ=255)

                                                                                          ccTGGTCTATTCTCTCTTCGCATAATACGTCCGGAAATATGGATAAAATTAAAATTAAGTTTGATTCATTAGCCTCTCACGATATTACCTTTGTTGGTATTGTACAGACCGCTAAAGCGTCCGGTATGGAACGTTT‑CCCg                                                 <  2:7056943/140‑1 (MQ=255)

                                                                                          ccTGGTCTATTCTCTCTTCGCATAATACGTCCGGAAATATGGATAAAATTAAAATTAAGTTTGATTCATTAGCCTCTCACGATATTACCTTTGTTGGTATTGTACAGACCGCTAAAGCGTCCGGTATGGAACGTTT‑CCCg                                                 <  1:2901264/140‑1 (MQ=255)

                                                                                          ccTGGTCTATTCTCTCGTCGCATAATACGTCCGGAAATATGGATAAAATTAAAATTAAGTTTGATTCATTAGCCTCTCACGATATTACCTTTGTTGGTATTGTACAGACCGCTAAAGCGTCCGGTATGGAACGTTT‑CCCg                                                 <  1:6504416/140‑1 (MQ=255)

                                                                                            tGGTCTATTCTCTCTTCGTATAATACGTCCGGAAATATGGATAAAATTAAAATTAAGTTTGATTCATTAGCCTCTCACGATATTACCTTTGTTGGTATTGTACAGACCGCTAAAGCGTCCGGTATGGAACGTTT‑CCCGCt                                               <  2:4866466/140‑1 (MQ=255)

                                                                                            tGGTCTATTCTCTCTTCGCATAATACGTCCGGAAATATGGATAAAATTAAAATTAAGTTTGATTCATTAGCCTCTCACGATATTACCTTTGTTGGTATTGTACAGACCGCTAAAGCGTCCGGTATGGAACGTTT‑CCCGCt                                               <  1:2192056/140‑1 (MQ=255)

                                                                                            tGGTCTATTCTCTCTTCGCATAATACGTCCGGAAATATGGATAAAATTAAAATTAAGTTTGATTCATTAGCCTCTCACGATATTACCTTTGTTGGTATTGTACAGACCGCTAAAGCGTCCGGTATGGAACGTTT‑CCCGCt                                               <  1:6084179/140‑1 (MQ=255)

                                                                                            tGGTCTATTCTCTCTTCGCATAATACGTCCGGAAATATGGATAAAATTAAAATTAAGATTGATTCATTAGCCTCTCACGATATTACCTTTGTTGGTATTGTACAGACCGCTAAAGCGTCCGGTATGGAACGTTT‑CCCGCt                                               <  2:2094096/140‑1 (MQ=255)

                                                                                               tCTATTCTCTCTTCGCATACTACGTCCGGAAATATGGATAAAATTAAAATTAAGTTTGATTCAGTAGCCTCTCACGATCTTACCTTTGTTGGTATTGTACAGACCGCTAAAGCGTCCGGTATGGAACGTTT‑CCCGCTgac                                            >  2:5860682/1‑138 (MQ=255)

                                                                                               tCTATTCTCTCTTCGCATAATACGTCCGGAAATCTGGATAAAATTAAAATTAAGTTTGATTCAGTAGCCTCTCACGATCTTACCTTTGTTGGTATTGTACCGACCGATAAAGCGTCCGGTATGGAACGTTA‑CCCGCTGcc                                            >  2:5633001/1‑140 (MQ=255)

                                                                                               tCTATTCTCTCTTCGCATAATACGTCCGGAAATATGGATAAAATTAAAATTAAGTTTGATTCATTAGCCTCTCACGATATTACCTTTGTTGGTATTGTACAGACCGCTAAAGCGTCCGGTATGGAACGTTT‑CCCGCTGcc                                            >  2:3640137/1‑140 (MQ=255)

                                                                                               tCTATTCTCTCTTCGCATAATACGTCCGGAAATATGGATAAAATTAAAATTAAGTTTGATTCATTAGCCTCTCACGATATTACCTTTGTTGGTATTGTACAGACCGCTAAAGCGTCCGGTATGGAACGTTT‑CCCGCTGcc                                            >  2:6128161/1‑140 (MQ=255)

                                                                                               tCTATTCTCTCTTCGCATAATACGTCCGGAAATATGGATAAAATTAAAATTAAGTTTGATTCATTAGCCTCTCACGATATTACCTTTGTTGGTATTGTACAGACCGCTAAAGCGTCCGGTATGGAACGTTT‑CCCGCTGcc                                            >  2:6543492/1‑140 (MQ=255)

                                                                                               tCTATTCTCTCTTCGCATAATACGTCCGGAAATATGGATAAAATTAAAATTAAGTTTGATTCATTAGCCTCTCACGATATTACCTTTGTTGGTATTGTACAGACCGCTAAAGCGTCCGGTATGGAACGTTT‑CCCGCTGcc                                            >  2:1841110/1‑140 (MQ=255)

                                                                                               tCTATTCTCTCTTCGCATAATACGTCCGGAAATATGGATAAAATTAAAATTAAGTTTGATTCATTAGCCTCTCACGATATTACCTTTGTTGGTATTGTACAGACCGCTAAAGCGTCCGGTATGGAACGTTT‑CCCGCTGcc                                            >  2:1966194/1‑140 (MQ=255)

                                                                                                cTATTCTCTCTTCGCATAATACGTCCGGAAATATGGATAAAATTAAAATTAAGTTTGATTCATTAGCCTCTCACGATATTACCTTTGTTGGTATTGTACAGACCGCTAAAGCGTCCGGTATGGAACGTTT‑CCCGCTGCCg                                           <  2:4123702/140‑1 (MQ=255)

                                                                                                 tcttCACTCTTAGCATACTACGTCTGTAAACATTGATCACATGCATCTTAAGTATGATTCATTAGCCTCTCACGATATTACCATTCATGGCATTGAACGGACCGCAAAAGCGTCCGGTATGTATCGTCA‑CCCGCCGCCGt                                          <  2:8010061/138‑1 (MQ=255)

                                                                                                 tATTCTCTCTTCGCATAATACGTCCGGAAATATGGATAAAATTAAAATTAAGTTTGATTCATTAGCCTCTCACGATATTACCTTTGTTGGTATTGTACAGACCGCTAAAGCGTCCGGTATGGAACGTTT‑CCCGCt                                               >  2:3836256/1‑135 (MQ=255)

                                                                                                 tATTCTCTCTTCGCATAATACGTCCGGAAATATGGATAAAATTAAAATTAAGTTTGATTCATTAGCCTCTCACGATATTACCTTTGTTGGTATTGTACAGACCGCTAAAGCGTCCGGTATGGAACGTTT‑CCCGCTGCCGt                                          <  1:1154052/140‑1 (MQ=255)

                                                                                                        tctTCGCATAATACGTCCGGAAATATGGATAAAATGAAATTTAAGTGTGGTTCATTGGCGTCTCACGATCTTCCCTTTGTTGGTATTGTTCAGACCGCTCATGCGTCGGGTATGGATGGGTT‑CCCGCTGCCGTGTGGGCt                                   >  1:6809604/1‑140 (MQ=255)

                                                                                                         ctTCGCATAATACGTCCGGAAATATGGATAAAATTAAAATTAAGTTTGATTCATTAGCCTCTCACGATATTACCTTTGTTGGTCTTGTACAGACCGCTAAAGCGTCCGGTATGGAACGTTT‑CCCGCTGCCGTATGTGCTg                                  >  2:4197701/1‑140 (MQ=255)

                                                                                                         ctTCGCATAATACGTCCGGAAATATGGATAAAATTAAAATTAAGTTTGATTCATTAGCCTCTCACGATATTACCTTTGTTGGTATTGTACAGACCGCTAAAGCGTCCGGTATGGAACGTTT‑CCCGCTGCCGTATGTGCTg                                  >  2:5305209/1‑140 (MQ=255)

                                                                                                         ctTCGCATAATACGTCCGGAAATATGGAAAAAATTAAAATTAAGTTTGATTCATTAGCCTCTCACGATATTACCTTTGTTGGTATTGTACAGACCGCTAAAGCGTCCGGTATGGAACGTTT‑CCCGCTGCCGTATGTGCTg                                  >  1:983503/1‑140 (MQ=255)

                                                                                                           tCGCATAATACGTCCGGAAATATGGATAAAATTAAAATTAAGTTTGATTCATTAGCCTCTCACGATATTACCTTTGTTGGTATTGTACAGACCGCTAAAGCGTCCGGTATGGAACGTTT‑CCCGCTGCCGTATGTGCTGAc                                >  1:2585544/1‑140 (MQ=255)

                                                                                                           tCGCATAATACGTCCGGAAATATGGATAAAATTAAAATTAAGTTTGATTCATTAGCCTCTCACGATATTACCTTTGTTGGTATTGTACAGACCGCTAAAGCGTCCGGTATGGAACGTTT‑CCCGCTGCCGTATGTGCTGAc                                >  1:873426/1‑140 (MQ=255)

                                                                                                             gCATAATACGTCCGGAAATATGGATAAAATTAAAATTAAGTTTGATTCATTAGCCTCTCACGATATTACCTTTGTTGGTATTGTACAGACCGCTAAGGCGTCCGGTATGGAACGTTT‑CCCGCTGCCGTATGTGCTGACCa                              >  2:7753200/1‑140 (MQ=255)

                                                                                                             gCATAATACGTCCGGAAATATGGATAAAATTAAAATTAAGTTTGATTCATTAGCCTCTCACGATATTACCTTTGTTGGTATTGTACAGACCGCTAAAGCGTCCGGTATGGAACGTTT‑CCCGCTGCCGTATGTGCTGACCa                              <  1:1093782/140‑1 (MQ=255)

                                                                                                              cATAAGACGTCCGGAACTATGGATAAAATGAAAATTAAGTGTGAGTCATTAGCCTCTCACGATATTACCTTTGTTGGTATTGTACAGACCGCTAAAGCGTCCGGTATGGAACGTTT‑CCCGCTGCCGTATGTGCTGACCaa                             <  2:6990614/140‑1 (MQ=255)

                                                                                                               ctcataCGGCCGGAAAAATGGATAAAATTAAAATTAATTTTGATTCATGAGCCTCTCACGATATTACCTTTGTTGGTATTGTACAGACCGCTAACGCGTCCGGTATGGAACGGGT‑CCCGCAGCCGTATGTGATGACCAAc                            <  2:5672659/137‑1 (MQ=255)

                                                                                                               ataataCGTCCGGAAATCTGGATAAAATTAAAATTAAGTTTGATTCATTAGCCTCTCACGATATTACCTTTGTTGGTATTGTACAGACCGCTAAAGCGTCCGGTATGGAACGTTT‑CCCGCTGCCGTATGTGCTGACCAAc                            <  2:7102784/140‑1 (MQ=255)

                                                                                                               ataataCGTCCGGAAATATGGATAAAATTAAAATTAAGTTTGATTCATTAGCCTCTCACGATATTACCTTTGTTGGTATTGTACAGACCGCTAAAGCGTCCGGTATGGAACGTTT‑CCCGCTGCCGTATGTGCTGACCAAc                            <  1:5068321/140‑1 (MQ=255)

                                                                                                                  ataCGTCCGGAAATATGGATAAAATTAAAATTAAGTTTGATTCATTAGCCTCTCACGATATTACCTTTGTTGGTCTTGTACAGACCGCTAAAGCGTCCGGTATGGAACGTTT‑CCCGCTGCCGTAGGTGCTGGCCAACTGc                         >  1:9230427/1‑140 (MQ=255)

                                                                                                                  ataCGTCCGGAAATATGGATAAAATTAAAATTAAGTTTGATTCATTAGCCTCTCACGATATTACCTTTGTTGGTATTGTACAGACCGCTAAAGCGTCCGGTATGGAACGTTT‑CCCGCTGCCGTATGTGCTGACCAACTGc                         >  1:3353752/1‑140 (MQ=255)

                                                                                                                   taCGTCCGGAAATATGGATAAAATTAAAATTAAGTTTGATTCATTAGCCTCTCACGATATTACCTTTGTTGGTATTGTACAGACCGCTAAAGCGTCCGGTATGGAACGTTT‑CCCGCTGCCGTATGTGCTGACCAACTGcc                        <  1:7756069/140‑1 (MQ=255)

                                                                                                                   taCGTCCGGAAATATGGATAAAATTAAAATTAAGTTTGATTCATTAGCCTCTCACGATATTACCTTTGTTGGTATTGTACAGACCGCTAAAGCGTCCGGTATGGAACGTTT‑CCCGCTGCCGTATGTGCTGACCAACTGcc                        <  2:1657478/140‑1 (MQ=255)

                                                                                                                   taCGTCCGGAAATATCGATAAAATTAAAAGTAAGTTAGATTCATTAGCCACTCACGATAGTACCTTTGTTGGTATTGTACCGACCGCTAAAGCGACCGGTATGGAACGTTT‑CCCGCTGCCGTATGTGCTGACCAACTGcc                        <  1:3617569/140‑1 (MQ=255)

                                                                                                                        ccGGAAATATGGATAAAATTAAAATTAAGTTTGATTCATTAGCCTCTCACGATATTACCTTTGTTGGTATTGTACAGACCGCTAAAGCGTCCGGTATGGAACGTTT‑CCCGCTGCCGTATGTGCTGACCAACTGCCATAac                   >  2:8880950/1‑140 (MQ=255)

                                                                                                                          ggAAATATGGATAAAATTACAATTAAGTTTGATTCATTAGCCTCTCACGATATTACCTTTGTTGGTATTGTACAGACCGCTAAAGCGTCCGGTATGGAACGTTT‑CCCGCTGCCGTATGTGCTGACCAACTGCCATAactc                 <  1:8423482/140‑1 (MQ=255)

                                                                                                                          ggAAATATGGATAAAATTAAAATTAAGTTTGATTCATTAGCCTCTCACGATATTACCTTTGTTGGTATTGTACAGACCGCTAAAGCGTCCGGTATGGAACGTTT‑CCCGCTGCCGTATGTGCTGACCAACTGCCATAactc                 <  1:9360907/140‑1 (MQ=255)

                                                                                                                           gAAATATGGATAAAATTAAAATTAAGTTTGATTCATTAGCCTCTCACGATATTACCTTTGTTGGTATTGTACAGACCGCTAAAGCGTCCGGTATGGAACGTTTCCCCGATGCCGTATCTGATGACCAACTGCCTTAactc                 >  2:7611708/1‑140 (MQ=255)

                                                                                                                           gAAATATGGATAAAATTAAAATTAAGTTTGATTCATTAGCCTCTCACGATATTACCTTTGTTGGTATTGTACAGACCGCTAAAGCGTCCGGTATGGAACGTTT‑CCCGCTGCCGTATGTGCTGACCAACTGCCATAactca                >  1:5271922/1‑140 (MQ=255)

                                                                                                                           gAAATATGGATAAAATTAAAATTAAGTTTGATTCATTAGCCTCTCACGATATTACCTTTGTTGGTATTGTACAGACCGCTAAAGCGTCCGGTATGGAACGTTT‑CCCGCTGCCGTATGTGCTGACCAACTGCCATAactca                >  1:2504666/1‑140 (MQ=255)

                                                                                                                             aaTATGGATAAAATTAAAATTAAGTTTGATTCATTAGCCTCTCACGATATTACCTTTGTTGGTATTGTACAGACCGCTAAAGCGTCCGGTATGGAACGTTT‑CCCGCTGCCGTATGTGCTGACCaa                             >  2:8723538/1‑125 (MQ=255)

                                                                                                                                atGGATAAAATTAAAATTAAGTTTGATTCATTAGCCTCTCACGATATTACCTTTGTTGGGATTGTACAGACCGCTAAAGCGTCCGGTATGGAACGTTT‑CCCGCTGCCGTATGTGCTGACCAACTGCCATAACTCACTCTg           >  2:8215313/1‑140 (MQ=255)

                                                                                                                                  tgATAAAATTAAAATTAAGTTTGATTCATTAGCCTCTCACGATATTACCTTTGTTGGTATTGTACAGACCGCTAAAGCGTCCGGTATGGAACGTTT‑CCCGCTGCCGTATGTGCTGACCAACTGCCATAACTCACTCTgcg         <  2:4057556/139‑1 (MQ=255)

GCGTGTTTCTCGCCAGTAATAACGAAATAATTGCCGAAGAACATTTCACCGGCGAAATTAAAAAAGAAGAAGCCAAAAAAGGCACTATTGCCTGGTCTATTCTCTCTTCGCATAATACGTCCGGAAATATGGATAAACTTAAAATTAAGTTTGATTCATTAGCCTCTCACGATATTACCTTTGTTGGTATTGTACAGACCGCTAAAGCGTCCGGTATGGAACGTTT‑CCCGCTGCCGTATGTGCTGACCAACTGCCATAACTCACTCTGCGCCGTCGG  >  NZ_CP025268/801425‑801701

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`