Predicted mutation
evidence seq id position mutation annotation gene description
RA NZ_CP025268 75,278 G→A R12C (CGC→TGC)  thiB ← thiamine ABC transporter substrate binding subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP02526875,2780GA99.7% 1380.1 / NA 358R12C (CGC→TGC) thiBthiamine ABC transporter substrate binding subunit
Reads supporting (aligned to +/- strand):  ref base G (1/0);  new base A (219/137);  total (220/138)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 364 total aligned reads displayed.

AGCGCCACCAGTTTCAGTTCGCAATTACAGTCGGCTTCAAAGGCTTTTTTAACCACCGGACCAGGCCCCCAGTCGGCGGCGAAGGAATCGTAGGTATAAACAGTCAGAACGGGTTTAGCGAAAACGGGCGCTGTGCGCAGCAACAGCAGGGGCAGACATTTTTTTAACACTTTGCACCTCAAAAAAGAGTGGCAAAGGACTTGAGAAGGAGCCTCAAATCCCTTCGCCGGCGTTATCCGGATCAGGTTCGACGGGTATTTTCTCAGCGCACGC  >  NZ_CP025268/75142‑75414
                                                                                                                                        |                                                                                                                                        
  cgcCACCAGTTTCAGTTCGCAATTACAGTCGGCTTCAAAGGCTTTTTTAACCACCGGACCAGGCCCCCAGTCGGCGGCGAAGGAATCGTAGGTATAAACAGTCAGAACGGGTTTAGCGAAAACGGGCGCTGTGCacagca                                                                                                                                     <  1:87898/140‑1 (MQ=255)
  cgcCACCAGTGTCAGTTCGCAATTACAGGCGGCTTCAAAGGCTTTTTTCACCACCGGACCAGGCCCCCAGTCGGCGGCGAAGGAATCGTAGGTATAAACAGTCAGAACGGGTTTAGCGAAAACGGGCGCTGTGCacagca                                                                                                                                     <  1:9505272/140‑1 (MQ=255)
    ccaccaGTTTCAGTTCGCAATTACAGTCGGCTTCAAAGGCTTTTTTAACCACCGGACCAGGCCCCCAGTCGGCGGCGAAGGAATCGTAGGTATAAACAGTCAGAACGGGTTTAGCGAAAACGGGCGCTGTGCACAGCAAc                                                                                                                                   <  2:5308545/140‑1 (MQ=255)
      cccaGTTTCAGTTCGCAATTACAGTCGGCTTCAAAGGCTTTTTTAACCACCGGACCAGGCCCCCAGTCGGCGGCGAAGGAATCGTAGGTATAAACAGTCAGAACGGGTTTAGCGAAAACGGGCGCTGTGCACAGCAAcag                                                                                                                                 <  1:5615291/139‑1 (MQ=255)
      accaGTTTCAGTTCGCAATTACAGTCGGCTTCAAAGGCTTTTTTAACCACCGGACCAGGCCCCCAGTCGGCGGCGAAGGAATCGTAGGTATAAACAGTCAGAACGGGTTTAGCGAACACGGGCGCTGTGCACAGCAAcag                                                                                                                                 >  1:7431541/1‑140 (MQ=255)
      accaGTTTCAGTTCGCAATTACAGTCGGCTTCAAAGGCTTTTTTAACCACCGGACCAGGCCCCCAGTCGGCGGCGAAGGAATCGTAGGTATAAACAGTCAGAACGGGTTTAGCGAAAACGGGCGCTGTGCACAGCAAcag                                                                                                                                 >  2:7033058/1‑140 (MQ=255)
      accaGTTTCAGTTCGCAATTACAGTCGGCTTAAAAGGCTTTTTTAACCTCCGGACCATGCCCCCAGTCGGCGGCGAAGGACTCGTCGGTCTACACCGTCAGACCGGGTTTAGGGATAGCGGGGGCTGTGCGCCGCGAcag                                                                                                                                 >  2:9050503/1‑140 (MQ=255)
      accaCTTTCAGTTCGCAATGACAGTCGGCTTCAAAGGCTATTTTAACCACCCGACCAGGCCCCCAGACGGCGGCGAAGGAATCGTAGGTATAAACAGTCAGAACGGGTTTAGCGAAAACGGGCGCTGTGCACAGCAAcag                                                                                                                                 <  1:8203589/140‑1 (MQ=255)
                gTTCGCAATTACAGTCGGCTTCAAAGGCTTTTTTAACCACCGGACCAGGCCCCCAGTCGGCGGCGAAGGAATCGTAGGTATAAACAGTCAGAACGGGTTTAGCGAAAACGGGCGCTGTGCACAGCAACAGCAGGGGCAGa                                                                                                                       >  1:8039008/1‑140 (MQ=255)
                   cGCAATTACAGTCGGCTTCAAAGGCTTTTTTAACCACCGGACCAGGCCCCCAGTCGGCGGCGAAGGAATCGTAGGTATAAACAGTCAGAACGGGTTTAGCGAAAACGGGCGCTGTGCACAGCAACAGCAGGGGCAGACAt                                                                                                                    <  2:2959578/140‑1 (MQ=255)
                   cGCAATTACAGTCGGCTTCAAAGGCTTTTTTAACCACCGGACCAGGCCCCCAGTCGGCGGCGAAGGAATCGTAGGTATAAACAGTCAGAACGGGTTTAGCGAAAACGGGCGCTGTGCACAGCAACAGCAGGGGCAGACAt                                                                                                                    <  2:3148071/140‑1 (MQ=255)
                   cGCAATTACAGTCGGCTTCAAAGGCTTTTTTAACCACCGGACCAGGCCCCCAGTCGGCGGCGAAGGAATCGTAGGTATAAACAGTCAGAACGGGTTTAGCGAAAACGGGCGCTGTGCACAGCAACAGCAGGGGCAGACAt                                                                                                                    <  2:4806869/140‑1 (MQ=255)
                     cAATTACAGTCGGCTTCAAAGGCTTTTTTAACCACCGGACCAGGCCCCCAGTCGGCGGCGAAGGAATCGTAGGTATAAACAGTCAGAACGGGTTTAGCGAAATCGGGCGCTGTGCACAGCAACAGCAGGGGCAGACAttt                                                                                                                  >  1:9200777/1‑140 (MQ=255)
                     cAATTACAGTCGGCTTCAAAGGCTTTTTTAACCACCGGACCAGGCCCCCAGTCGGCGGCGAAGGAATCGTAGGTATAAACAGTCAGAACGGGTTTAGCGAAAACGGGCGCTGTGCACAGCAACAGCAGGGGCAGACAttt                                                                                                                  >  1:4435052/1‑140 (MQ=255)
                          aCAGTCGGCTTCAAAGGCTTTTTTAACCACCGGACCAGGCCCCCAGTCGGCGGCGAAGGAATCGTAGGTATAAACAGTCAGAACGGGTTTAGCGAAAACGGGCGCTGTGCACAGCAACAGCAGGGGCAGACATTTTTTTa                                                                                                             <  1:1018216/140‑1 (MQ=255)
                                  cTTCAAAGGCTTTTTTAACCACCGGACCAGGCCCCCAGTCGGCGGCGAAGGAATCGTAGGTATAAACAGTCAGAACGGGTTTAGCGAAAACGGGCGCTGTGCACAGCAACAGCAGGGGCAGACATTTTTTTAACACTTTg                                                                                                     >  2:4335563/1‑140 (MQ=255)
                                  cTTCAAAGGCTTTTTTAACCACCGGACCAGGCCCCCAGTCGGCGGCGAAGGAATCGTAGGTATAAACAGTCAGAACGGGTTTAGCGAAAACGGGCGCTGTGCACAGCAACAGCAGGGGCAGACATTTTTTTAACACTTTg                                                                                                     <  1:4282396/140‑1 (MQ=255)
                                  cTTCAAAGGCTTTTTTAACCACCGGACCAGGCCCCCAGTCGGCGGCGAAGGAATCGTAGGTATAAACAGTCAGAACGGGTTTAGCGAAAACGGGCGCTGTGCACAGCAACAGCAGGGGCAGACATTTTTTTAACACTTTg                                                                                                     <  1:4808697/140‑1 (MQ=255)
                                    tCAAAGGCTTTTTTAACCACCGGACCAGGCCCCCAGTCGGCGGCGAAGGAATCGTAGGTATAAACAGTCAGAACGGGTTTAGCGAAAACGGGCGCTGTGCACAGCAACAGCAGGGGCAGACATTTTTTTAACACTTTGCa                                                                                                   <  2:5618051/140‑1 (MQ=255)
                                      aaaGGCTTTTTTAACCACCGGACCAGGCCCCCAGTCGGCGGCGAAGGAATCGTAGGTATAAACAGTCAGAACGGGTTTAGCGAAAACGGGCGCTGTGCACAGCAACAGCAGGGGCAGACATTTTTTTAACACTTTGCAcc                                                                                                 <  2:1423876/140‑1 (MQ=255)
                                        aGGCTTTTTTAACCACCGGACCAGGCCCCCAGTCGGCGGCGAAGGAATCGTAGGTATAAACAGTCAGAACGGGTTTAGCGAAAACGGGCGCTGTGCACAGCAACAGCAGGGGCAGACATTTTTTTAACACTTTGCACCTc                                                                                               >  2:6663635/1‑140 (MQ=255)
                                          gCTTTTTTAACCACCGGACCAGGCCCCCAGTCGGCGGCGAAGGAATCGTAGGTATAAACAGTCAGAACGGGTTTAGCGAAAACGGGCGCTGTGCACAGCAACAGCAGGGGCAGACATTTTTTTAACACTTTGCACCTCaa                                                                                             >  1:6364996/1‑140 (MQ=255)
                                            ttttttAACCACCGGACCAGGCCCCCAGTCGGCGGCGAAGGACTCGTAGGTATAAACAGTCAGAACGGGTTTAGCGAAAACGGGCGCTGTGCACAGCAACAGCAGGGGCAGACATTTTTTTAACACTTTGCACCTCaaaa                                                                                           >  2:3644530/1‑140 (MQ=255)
                                                 tAACCACCGGACCAGGCCCCCAGTCGGCGGCGAAGGAATCGTAGGTATAAACAGTCAGAACGGGTTTAGCGAAAACGGGCGCTGTGCACAGCAACAGCAGGGGCAGACATTTTTTTAACACTTTGCACCTCAAAAAagag                                                                                      <  1:3125311/140‑1 (MQ=255)
                                                 tAACCACCGGACCAGGCCCCCAGTCGGCGGCGAAGGAATCGTAGGTATAAACAGTCAGAACGGGTTTAGCGAAAACGGGCGCTGTGCACAGCAACAGCAGGGGCAGACATTTTTTTAACACTTTGCACCTCAAAAAagag                                                                                      <  2:759307/140‑1 (MQ=255)
                                                      accGGACCAGGCCCCCAGTCGGCGGCGAAGGAATCGTAGGTATAAACAGTCAGAACGGGTTTATCGAAAACGGGCGCTGTGCACAGCAACAGCAGGGGCAGACATTTTTTTCACACTTTGCACCTCAAAAAAGAGTGGCa                                                                                 <  2:5759472/140‑1 (MQ=255)
                                                        cGGACCAGGCCCCCAGTCGGCGGCGAAGGACTCGTAGGTATACACAGTCAGAACGGGTTTAGCGAAAACGGGCGCTGTGCAGAGCAACAGCAGGGGCAGACATTTTTTTCACACTTTGCACCTCCCCACAGAGTGGCaga                                                                               >  2:6699908/1‑138 (MQ=255)
                                                         ggACCAGGCCCCCAGGAGGCGGCGAAGGATTAGTAGGTATAAACAGTCAGAAAGGGTTTAGCTAAAACGGGCGCTGTGCACTTCTACAGCAGTGGCATAAATTTTTTTAACACTTTGCACCTCAAAAAAGAGTGGCaatg                                                                              <  2:3032976/140‑3 (MQ=255)
                                                          gACCAGGCCCCCAGTCGGCGGCGAAGGAATCGTAGGTATAAACAGTCAGAACGGGTTTAGCGAAAACGGGCGCTGTGCACAGCAACAGCAGGGGCAGACATTTTTTTAACACTTTGCACCTCAAAAAAGAGTGGCAAAgg                                                                             <  1:192710/140‑1 (MQ=255)
                                                          gACCAGGCCCCCAGTCGGCGGCGAAGGAATCGTAGGTATAAACAGTCAGAACGGGTTTAGCGAAAACGGGCGCTGTGCACAGCAACAGCAGGGGCAGACATTTTTTTAACACTTTGCACCTCAAAAAAGAGTGGCAAAgg                                                                             <  1:4021171/140‑1 (MQ=255)
                                                           aCCAGGCCCCCAGTCGGCGGCGAAGGAATCGTAGGTATAAACAGTCAGAACGGGTTTAGCGAAAACGGGCGCTGTGCACAGCAACAGCAGGGGCAGACATTTTTTTAACACTTTGCACCTCAAAAAAGAGTGGCAAAGGa                                                                            <  1:2769814/140‑1 (MQ=255)
                                                               ggCCCCCAGTCGGCGGCGAAGGTATCGTAGGTAAAAACAGTCAGAACGGGTTTAGCGAAAACGGGCGCTGTGCACAGCAACAGCAGGGGCAGACATTTTTTTAACACTTTGCACCTCAAAATAGAGTGGCAAGGGACGTg                                                                        >  1:7840072/1‑140 (MQ=255)
                                                               ggCCCCCAGTCGGCGGCGAAGGAATCGTAGGTCTAAACAGTCAGAACGGGTTTAGCGAAAACGGGCGCTGTGCACAGCAACAGCAGGGGCAGACATTTTTTTAACACTTTGCACCTCAAAAAAGAGTGGCAAAGGACTTg                                                                        >  1:7723809/1‑140 (MQ=255)
                                                               ggCCCCCAGTCGGCGGCGAAGGAATCGTAGGTATAAACAGTCAGAACGGGTTTAGCGAAAACGGGCGCTGTGCACAGCAACAGCAGGGGCAGACATTTTTTTAACACTTTGCACCTCAAAAAAGAGTGGCAAAGGACTTg                                                                        >  2:2034815/1‑140 (MQ=255)
                                                               ggCCCCCAGTCGGCGGCGAAGGAATCGTAGGTATAAACAGTCAGAACGGGTTTAGCGAAAACGGGCGCTGTGCACAGCAACAGCAGGGGCAGACATTTTTTTAACACTTTGCACCTCAAAAAAGAGTGGCAAAGGACTTg                                                                        >  1:4569833/1‑140 (MQ=255)
                                                               ggCCCCCAGTCGGCGGCGAAGGAATCGTAGGTATAAACAGTCAGAACGGGTTTAGCGAAAACGGGCGCTGTGCACAGCAACAGCAGGGGCAGACATTTTTTTAACACTTTGCACCTCAAAAAAGAGTGGCAAAGGACTTg                                                                        >  1:110980/1‑140 (MQ=255)
                                                               ggCCCCCAGTCGGCGGCGAAGGAATCGTAGGTATAAACAGTCAGAACGGGTTTAGCGAAAACGGGCGCTGTGCACAGCAACAGCAGGGGCAGACATTTTTTTAACACTTTGCACCTCAAAAAAGAGAGGCAAAGGACTTg                                                                        >  1:7913577/1‑140 (MQ=255)
                                                                  ccccAGTCGGCGGCGAAGGAATCGTAGGTATAAACAGTCAGAACGGGTTTAGCGAAAACGGGCGCTGTGCACAGCAACAGCAGGGGCAGACATTTTTTTAACACTTTGCACCTCAAAAAAGAGTGGCAAAGGACTTgaga                                                                     >  2:7767873/1‑140 (MQ=255)
                                                                    ccAGTCGGCGGCGAAGGAATCGTAGGTATAAACAGTCAGAACGGGTTTAGCGACAACGGGCGCTGTGCACAGCAACAGCAGGGGCAGACATTTTTTTAACACTTTGCACCTCAAAAAAGAGTGGCAAAGGACTTGAGAAg                                                                   >  1:1317651/1‑140 (MQ=255)
                                                                    ccAGTCGGCGGCGAAGGAATCGTAGGTATAAACAGTCAGAACGGGTTTAGCGAAAACGGGCGCTGTGCACAGCAACAGCAGGGGCAGACATTTTTTTAACACTTTGCACCTCAAAAAAGAGTGGCAAAGGACTTGAGAAg                                                                   >  1:6245028/1‑140 (MQ=255)
                                                                     cAGTCGGCGGCGAAGGAATCGTAGGTATAAACAGTCAGAACGGGTTTAGCGAAAACGGGCGCTGTGCACAGCAACAGCAGGGGCAGACATTTTTTTAACACTTTGCACCTCAAAAAAGAGTGGCAAAGGACTTGAGAAgg                                                                  >  2:7208488/1‑140 (MQ=255)
                                                                     cAGTCGGCGGCGAAGGAATCGTAGGTATAAACAGTCAGAACGGGTTTAGCGAAAACGGGCGCTGTGCACAGCAACAGCAGGGGCAGACATTTTTTTAACACTTTGCACCTCAAAAAAGAGTGGCAAAGGACTTGAGAAgg                                                                  >  2:6487985/1‑140 (MQ=255)
                                                                      aGTCGGCGGCGAAGGAATCGTAGGTATAAACAGTCAGAACGGGTTTAGCGAAAACGGGCGCTGTGCACAGCAACAGCAGGGGCAGACATTTTTTTAACACTTTGCACCTCAAAAAAGAGTGGCAAAGGACTTGAGAAGGa                                                                 <  2:7505050/140‑1 (MQ=255)
                                                                          ggcggcGAAGGAATCGTAGGTATAAACAGTCAGAACGGGTTTAGCGAAAACGGGCGCGGTGCACAGCAACAGCAGGGGCAGACATTTTTTTAACACTTTGCACCTCACAAAAGAGTGGCAAAGGACTTGAGAAGGAGCCt                                                             >  2:3499792/1‑140 (MQ=255)
                                                                             ggcgAAGGAATCGTAGGTATAAACAGTCAGAACGGGTTTAGCGAAAACGGGCGCTGTGCACAGCAACAGCAGGGGCAGACATTTTTTTAACACTTTGCACCTCAAAAAAGAGTGGCAAAGGACTTGAGAAGGAGCCTCaa                                                          >  2:2016155/1‑140 (MQ=255)
                                                                             ggcgAAGGAATCGTAGGTAGACACAGTCAGAACGGGTTTAGCGAAAACGGGCGCTGTGCACAGCAACAGCAGGGGCAGACATTTATTTTACACTTTGCTCCTCAAAATTGTGTGGCAAAGTACTTGAGAAGGAGCCTCaa                                                          >  2:8894466/1‑140 (MQ=255)
                                                                                  aGGAATCGTAGGTATAAACAGTCAGAACGGGTTTAGCGAAAACGGGCGCTGTGCACAGCAACAGCAGGGGCAGACATTTTTTTAACACTTTGCACCTCAAAAAAGAGTGGCAAAGGACTTGAGAAGGAGCCTCAAATccc                                                     >  2:7669020/1‑140 (MQ=255)
                                                                                  aGGAATCGTAGGTATAAACAGTCAGAACGGGTTTAGCGAAAACGGGCGCTGTGCACAGCAACAGCAGGGGCAGACATTTTTTTAACACTTTGCACCTCAAAAAAGAGTGGCAAAGGACTTGAGAAGGAGCCTCAAATccc                                                     >  2:6952165/1‑140 (MQ=255)
                                                                                      aTCGTAGGTATAAACAGTCAGAACGGGTTTAGCGAAAACGGGCGCTGTGCACAGCAACAGCAGGGGCAGACATTTTTTTAACACTTTGCACCTCAAAAAAGAGTGGCAAAGGACTTGAGAAGGAGCCTCAAATCCCTTCg                                                 <  2:5437655/140‑1 (MQ=255)
                                                                                       tCGTAGGTATAAACAGTCAGAACGGGTTTAGCGAAAACGGGCGCTGTGCACAGCAACAGCAGGGGCAGACATTTTTTTAACACTTTGCACCTCAAAAAAGAGTGGCAAAGGACTTGAGAAGGAGCCTCAAATCCCTTCGc                                                <  1:3959066/140‑1 (MQ=255)
                                                                                       tCGTAGGTATAAACAGTCAGAACGGGTTTAGCGAAAACGGGCGCTGTGCACAGCAACAGCAGGGGCAGACATTTTTTTAACACTTTGCACCTCAAAAAAGAGTGGCAAAGGACTTGAGAAGGAGCCTCAAATCCCTTCGc                                                >  2:6131177/1‑140 (MQ=255)
                                                                                       tCGTAGGTATAAACAGTCAGAACGGGTTTAGCGAAAACGGGCGCTGTGCACAGCAACAGCAGGGGCAGACATTTTTTTAACACTTTGCACCTCAAAAAAGAGTGGCAAAGGACTTGAGAAGGAGCCTCAAATCCCTTCGc                                                >  2:5944533/1‑140 (MQ=255)
                                                                                       tCGTAGGTATAAACAGTCAGAACGGGTTGAGCGAAAACGGGCGCGGTGCACAGCAACAGCAGGGGCAGACATTTTTTTAACCCTTTGCACCTCAACAAAGAGTGGCAAAGGACTTGAGAAGGAGCCTCAAATCCCTTCGc                                                <  2:7405694/140‑1 (MQ=255)
                                                                                         gTAGGTATAAACAGTCAGAACGGGTTTAGCGAAAACGGGCGCTGTGCACAGCAACAGCAGGGGCAGACATTTTTTTGACACTTTGCACCTCAAAAAAGAGTGGCAAAGGACTTGAGAAGGAGCCTCAAATCCCTTCGCCg                                              >  2:4747025/1‑140 (MQ=255)
                                                                                            ggTATAAACAGTCAGAACGGGTTTAGCGAAAACGGGCGCTGTGCACAGCAACAGCAGGGGCAGACATTTTTTTAACACTTTGCACCTCAAAAAAGAGTGGCAAAGGACTTGAGAAGGAGCCTCAAATCCCTTCGCCGGCg                                           >  2:5106636/1‑140 (MQ=255)
                                                                                            ggTATAAACAGTCAGAACGGGTTTAGCGAAAACGGGCGCTGTGCACAGCAACAGCAGGGGCAAACATTTTTTTAACACTTTGCACCTCAAAAAAGAGTGGCAAAGGACTTGAGAAGGAGCCTCAAATCCCTTCGCCGGCg                                           <  2:3706009/140‑1 (MQ=255)
                                                                                              tataAACAGTCAGAACGGGTTTAGCGAAAACGGGCGCTGTGCACAGCAACAGCAGGGGCAGACATTTTTTTAACACTTTGCACCTCAAAAAAGAGTGGCAAAGGACTTGAGAAGGAGCCTCAAATCCCTTCGCCGGCGtt                                         >  1:908212/1‑140 (MQ=255)
                                                                                              tataAACAGTCAGAACGGGTTTAGCGAAAACGGGCGCTGTGCACAGCAACAGCAGGGGAAGTCATTTTTTTAACACTTTGCTCCTCAAAAAAGAGTGGCTATGGACTTGAGAAGGTGACTCAAATCGCTTCGCCGGCGtt                                         >  2:1762729/1‑140 (MQ=255)
                                                                                               ataAACAGTCAGAACGGGTTTAGCGAAAACGGGCGCTGTGCACAGCAACAGCAGGGGCAGACATTTTTTTAACACTTTGCACCTCAAAAAAGAGTGGCAAAGGACTTGATAAGGAGCCTCAAATCCCTTCGCCGGCGTTa                                        >  1:8743611/1‑140 (MQ=255)
                                                                                               ataAACAGTCAGAACGGGTTTAGCGAAAACGGGCGCTGTGCACAGCAACAGCAGGGGCAGACATTTTTTTAACACTTTGCACCTCAAAAAAGAGTGGCAAAGGACTTGAGAAGGAGCCTCAAATCCCTTCGCCGGCGGTa                                        >  2:6203798/1‑140 (MQ=255)
                                                                                                taAACAGTCAGAACGGGTTTAGCGAAAACGGGCGCTGTGCACAGCAACAGCAGGGGCAGACATTTTTTTAACACTTTGCACCTCAAAAAAGAGTGGCAAAGGACTTGAGAAGGAGCCTCAAATCCCTTCGCCGGCGTTAt                                       <  2:7962240/140‑1 (MQ=255)
                                                                                                 aaaCAGTCAGAACGTGTTTAGCGAAAACGGTCGCTGTGCACAGCAACAGCAGGGGCAGACATTTTTTTAACACTTTGCACCTCAAAAAAGAGTGGCAAAGGACTTGAGAAGGAGCCTCAAATCCCTTCGCCGGCGTTATc                                      >  2:4946571/1‑140 (MQ=255)
                                                                                                 aaaCAGTCAGAACGGGTTTAGCGACAACGGGCGCTGTGCCCAGCAACAGCAGGGGCAGACATTTTTTTAACACTTTGCACCTCAAAAAAGAGTGGCAAAGGACTTGAGAAGGAGCCTCAAATCCCTTCGCCGGCGTTATc                                      <  2:9329646/140‑1 (MQ=255)
                                                                                                 aaaCAGTCAGAACGGGTTTAGCGAAAACGGGCGCTGTGCACAGCAACAGCAGGGGCAGACATTTTTTTAACACTTTGCACCTCAAAAAAGAGTGGCAAAGGACTTGAGAAGGAGCCTCAAATCCCTTCGCCGGCGTTATc                                      <  2:8147738/140‑1 (MQ=255)
                                                                                                  aaCAGTCAGAACGGGTTTAGCGAAAACGGGCGCTGTGCACAGCAACAGCAGGGGCAGACATTTTTTTAACACTTTGCACCTCAATAAAGAGTGGCAAAGGACTTGAGAAGGAGCCTCAAATCCCTTCGCCGGCGTTATcc                                     >  2:1302845/1‑140 (MQ=255)
                                                                                                      gTCAGAACGGGTTTAGCGAAAACGGGCGCTGTGCACAGCAACAGCAGGGGCAGACATTTTTTTAACACTTTGCACCTCAAAAAAGAGTGGCAAAGGACTTGAGACGGAGCCTCAAATCCCTTCGCCGGCGTTATCCGGAt                                 <  2:2756383/140‑1 (MQ=255)
                                                                                                       tCAGAACGGGTTTAGCGACAACGGGCGCTGTGCACAGCAACAGCAGGGGCCGACATTTTTTTAACACTTTGCACCTCAAAAAAGAGTGGCAAAGGACTTGAGAAGGAGCCTCAAATCCCTTCGCCGGCGTTATCCGGATc                                >  1:5794891/1‑140 (MQ=255)
                                                                                                       tCAGAACGGGTTTAGCGAACACGGGCGCTGTGCACAGCAACAGCAGGGGCAGACATTTTTTTAACACTTTGCACCTCAAAAAAGAGTGGCAAAGGACTTGAGAAGGAGCCTCAAATCCCTTCGCCGGCGTTATCCGGATc                                >  1:2129957/1‑140 (MQ=255)
                                                                                                       tCAGAACGGGTTTAGCGAAAACGGGCGCTGTGCACAGCAACAGCAGGGGCAGACATTTTTTTAACACTTTGCACCTCAAAAAAGAGTGGCAAAGGACTTGAGAAGGAGCCTCAAATCCCTTCGCCGGCGTTATCCGGATc                                >  1:381163/1‑140 (MQ=255)
                                                                                                       tCAGAACGGGTTTAGCGAAAACGGGCGCTGTGCACAGCAACAGCAGGGGCAGACATTTTTTTAACACTTTGCACCTCAAAAAAGAGTGGCAAAGGACTTGAGAAGGAGCCTCAAATCCCTTCGCCGGCGTTATCCGGATc                                >  1:7144538/1‑140 (MQ=255)
                                                                                                       tCAGAACGGGTTTAGCGAAAACGGGCGCTGTGCACAGCAACAGCAGGGGCAGACATTTTTTTAACACTTTGCACCTCAAAAAAGAGTGGCAAAGGACTTGAGAAGGAGCCTCAAATCCCTTCGCCGGCGTTATCCGGATc                                >  1:263952/1‑140 (MQ=255)
                                                                                                       tCAGAACGGGTTTAGCGAAAACGGGCGCTGTGCACAGCAACAGCAGGGGCAGACATTTTTTTAACACTTTGCACCTCAAAAAAGAGTGGCAAAGGACTTGAGAAGGAGCCTCAAATCCCTTCGCCGGCGTTATCCGGATc                                >  1:933419/1‑140 (MQ=255)
                                                                                                       tCAGAACGGGTTTAGCGAAAACGGGCGCTGTGCACAGCAACAGCAGGGGCAGACATTTTTTTAACACTTTGCACCTCAAAAAAGAGTGGCAAAGGACTTGAGAAGGAGCCTCAAATCCCTTCGCCGGCGTTATCCGGATc                                >  1:1469906/1‑140 (MQ=255)
                                                                                                       tCAGAACGGGTTTAGCGAAAACGGGCGCTGTGCACAGCAACAGCAGGGGCAGACATTTTTTTAACACTTTGCACCTCAAAAAAGAGTGGCAAAGGACTTGAGAAGGAGCCTCAAATCCCTGCGCCGGCGTGATCCGGATc                                >  1:828023/1‑140 (MQ=255)
                                                                                                       tCAGAACGGGTTTAGCGAAAACGGGCGCTGTGCACAGCAACAGCAGGGGCAGACATTTTTTTAACACTTTGCACCTCAAAAAAGAGTGGCAAAGGACTTGAGAAGGAGCCTCAAATCCCGTCGCCGGCGTTATCCGGagc                                >  2:4635208/1‑138 (MQ=255)
                                                                                                       tCAGAACGGGTTTAGCGAAAACGGGCGCTGTGCACAGCAACAGCAGGGGAAGACATTTTTTTAACACTTTGCACCTCAAAAAAGAGTGGCAAAGGACTTGAGAAGGAGCCTCAAATCCCTTCGCCGGCGATATCCGGATc                                >  2:9302915/1‑140 (MQ=255)
                                                                                                        cAGAACGGGTTTAGCGAAAACGGGCGCTGTGCACAGCAACAGCAGGGGCAGACATTTTTTTAACACTTTGCACCTCAAAAAAGAGTGGCAAAGGACTTGAGAAGGAGCCTCAAATCCCTTCGCCGGCGTTATCCGGATCa                               >  1:5489092/1‑140 (MQ=255)
                                                                                                        cAGAACGGGTTTAGCGAAAACGGGCGCTGTGCACAGCAACAGCAGGGGCAGACATTTTTTTAACACTTTGCACCTCAAAAAAGAGTGGCAAAGGACTTGAGAAGGAGCCTCAAATCCCTTCGCCGGCGTTATCCGGATCa                               >  2:3868310/1‑140 (MQ=255)
                                                                                                           aaCGGGTTTAGCGAAAACGGGCGCTGTGCACAGCAACAGCAGGGGCAGACATTTTTTTAACACTTTGCACCTCAAAAAAGAGTGGCAAAGGACTTGAGAAGGAGCCTCAAATCCCTTCGCCGGCGTTATCCGGATCAGGt                            <  1:6592463/140‑1 (MQ=255)
                                                                                                               ggTTTAGCGAAAACGGGCGCTGTGCACAGCAACAGCAGGGGCAGACATTTTTTTAACACTTTGCACCTCAAAAAAGAGTGGCAAAGGACTTGAGAAGGAGCCTCAAATCCCTTCGCCGGCGTTATCCGGATCAGGTTCGa                        <  1:7108913/140‑1 (MQ=255)
                                                                                                               ggTTTAGCGAAAACGGGCGCTGTGCACAGCAACAGCAGGGGCAGACATTTTTTTAACACTTTGCACCTCAAAAAAGAGTGGCAAAGGACTTGAGAAGGAGCCTCAAATCCCTTCGCCGGCGTTATCCGGATCAGGTTCGa                        <  2:4422840/140‑1 (MQ=255)
                                                                                                               ggTTTAGCGAAAACGGGCGCTGTGCACAGCAACAGCAGGGGCAGACATTTTTTTAACACTTTGCACCTCAAAAAAGAGTGGCAAAGGACTTGAGAAGGAGCCTCAAATCCCTTCGCCGGCGTTATCCGGATCAGGGTCGa                        >  1:6124322/1‑140 (MQ=255)
                                                                                                                 tttAGCGAAAACGGGCGCTGTGCACAGCAACAGCAGGGGCAGACATTTTTTTAACACTTTGCACCTCAAAAAAGAGTGGCAAAGGACTTGAGAAGGAGCCTCAAATCCCTTCGCCGGCGTTATCCGGATCAGGTTCGACg                      <  1:6985952/140‑1 (MQ=255)
                                                                                                                 tttAGCGAAAACGGGCGCTGTGCACAGCAACAGCAGGGGCAGACATTTTTTTAACACTTTGCACCTCAAAAAAGAGTGGCAAAGGACTTGAGAAGGAGCCTCAAATCCCTTCGCCGGCGTTATCCGGATCAGGTTCGACg                      >  2:8593677/1‑140 (MQ=255)
                                                                                                                 tttAGCGAAAACGGGCGCTGTGCACAGCAACAGCAGGGGCAGACATTTTTTTAACACTTTGCACCTCAAAAAAGAGTGGCAAAGGACTTGAGAAGGAGCCTCAAATCCCTTCGCCGGCGTTATCCGGATCAGGTTCGACg                      <  1:8650214/140‑1 (MQ=255)
                                                                                                                     gCGAAAACGGGCGCTGTGCACAGCAACAGCAGGGGCAGACATTTTTTTAACACTTTGCACCTCAAAAAAGAGTGGCAAAGGACTTGAGAAGGAGCCTCAAATCCCTTCGCCGGCGTTATCCGGATCAGGTTCGACGGGTa                  <  1:5305347/140‑1 (MQ=255)
                                                                                                                          aaCGGGCGCTGTGCACAGCAACAGCAGGGGCAGACATTTTTTTAACACTTTGCACCTCAAAAAAGAGTGGCAAAGGACTTGAGAAGGAGCCTCAAATCCCTTCGCCGGCGTTATCCGGATCAGGTTCGACGGGTATTTtc             >  2:519194/1‑140 (MQ=255)
                                                                                                                          aaCGGGCGCTGTGCACAGCAACAGCAGGGGCAGACATTTTTTTAACACTTTGCACCTCAAAAAAGAGTGGCAAAGGACTTGAGAAGGAGCCTCAAATCCCTTCGCCGGCGTTATCCGGATCAGGTTCGACGGGTATTTtc             >  2:8390645/1‑140 (MQ=255)
                                                                                                                          aaCGGGCGCTGTGCACAGCAACAGCAGGGGCAGACATTTTTTTAACACTTTGCACCTCAAAAAAGAGTGGCAAAGGACTTGAGAAGGAGCCTCAAATCCCTTCGCCGGCGTTATCCGGATCAGGTTCGACGGGTATTTtc             >  2:8820212/1‑140 (MQ=255)
                                                                                                                          aaCGGGCGCTGTGCACAGCAACAGCAGGGGCAGACATTTTTTTAACACTTTGCACCTCAAAAAAGAGTGGCAAAGGACTTGAGAAGGAGCCTCAAATCCCTTCGCCGGCGTTATCCGGATCAGGTTCGACGGGTATTTtc             >  2:3989574/1‑140 (MQ=255)
                                                                                                                          aaCGGGCGCTGTGCACAGCAACAGCAGGGGCAGACATTTTTTTAACACTTTGCACCTCAAAAAAGAGTGGCAAAGGACTTGAGAAGGAGCCTCAAATCCCTTCGCCGGCGTTATCCGGATCAGGTGCGACGGGGAGTTtc             >  1:1833726/1‑140 (MQ=255)
                                                                                                                              ggCGCTGTGCACAGCAACAGCATGTGAATACATTTTTTTAACACTTTGCAACTAAAAAAAGAGTGGCAAAGGACTTGAGAAGGAGCCTCAAATACATTCGCCGGCTTTATCAGGATCAGGTTCGACGTGTATTTTCTCAg         >  1:7250274/1‑140 (MQ=255)
                                                                                                                               gcgcTGTGCACAGCAACAGCAGGGGCAGACATTTTTTTAACACTTTGCACCTCAAAAAAGAGTGGCAAAGGACTTGAGAAGGAGCCTCAAATCCCTTCGCCGGCGTTATCCGGATCAGGTTCGACGGGTATTTTCTCAgc        >  2:229462/1‑140 (MQ=255)
                                                                                                                                cgcTGTGCACAGCAACAGCAGGGGCAGACATTTTTTTAACACTTTGCACCTCAAAAAAGAGTGGCAAAGGACTTGAGAAGGAGCCTCAAATCCCTTCGCCGGCGTTATCCGGATCAGGTTCGACGGGTATTTTCTCAgcg       <  1:6747561/140‑1 (MQ=255)
                                                                                                                                cgcTGTGCACAGCAACAGCAGGGGCAGACATTTTTTTAACACTTTGCACCTCAAAAAAGAGTGGCAAAGGACTTGAGAAGGAGCCTCAAATCCCTTCGCCGGCGTTATCCGGATCAGGTTCGACGGGTATTTTCTCAgcg       <  1:373534/140‑1 (MQ=255)
                                                                                                                                cgcTGTGCACAGCAACAGCAGGGGCAGACATTTTTTTAACACTTTGCACCTCAAAAAAGAGTGGCAAAGGACTTGAGAAGGAGCCTCAAATCCCTTCGCCGGCGTTATCCGGATCAGGTTCGACGGGTATTTTCTCAgcg       <  1:5287266/140‑1 (MQ=255)
                                                                                                                                 gcTGTGCACAGCAACAGCAGGGGCAGACATTTTTTTAACACTTTGCACCTCAAAAAAGAGTGGCAAAGGACTTGAGAAGGAGCCTCAAATCCCTTCGCCGGCGTTATCCGGATCAGGTTCGACGGGTATTTTCTCAgcgc      <  1:236449/140‑1 (MQ=255)
                                                                                                                                  cTGTGCACAGCAACAGCAGGGGCAGACATTTTTTTAACACTTTGCACCTCAAAAACGAGTGGCAAAGGACTTGAGAAGGAGCCTCAAATCCCTTCGCCGGCGTTATCCGGATCAGGTTCGACGGGTATTTTCTCAGCGCa     <  2:588205/140‑1 (MQ=255)
                                                                                                                                    gtgCACAGCAACAGCAGGGGCAGACATTTTTTTAACACTTTGCACCTCAAAAAAGAGTGGCAAAGGACTTGAGAAGGAGCCTCAAATCCCTTCGCCGGCGTTATCCGGATCAGGTTCGACGGGTATTTTCTCAGCGCacg   <  2:9148493/140‑1 (MQ=255)
                                                                                                                                     tgCACAGCAACAGCAGGGGCAGACATTTTTTTAACACTTTGCACCTCAAAAAAGAGTGGCAAAGGACTTGAGAAGGAGCCTCAAATCCCTTCGCCGGCGTTATCCGGATCAGGTTCGACGGGTATTTTCTCAGCGCacgc  <  2:2498163/140‑1 (MQ=255)
                                                                                                                                        |                                                                                                                                        
AGCGCCACCAGTTTCAGTTCGCAATTACAGTCGGCTTCAAAGGCTTTTTTAACCACCGGACCAGGCCCCCAGTCGGCGGCGAAGGAATCGTAGGTATAAACAGTCAGAACGGGTTTAGCGAAAACGGGCGCTGTGCGCAGCAACAGCAGGGGCAGACATTTTTTTAACACTTTGCACCTCAAAAAAGAGTGGCAAAGGACTTGAGAAGGAGCCTCAAATCCCTTCGCCGGCGTTATCCGGATCAGGTTCGACGGGTATTTTCTCAGCGCACGC  >  NZ_CP025268/75142‑75414

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑