BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP025268	1,075,358	A→G	58 (TAA→TAG)	ymdF →	general stress protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP025268	1,075,358	0	A	G	99.8%	1515.5 / NA	444	58 (TAA→TAG)	ymdF	general stress protein
Reads supporting (aligned to +/- strand): ref base A (0/0); major base G (186/257); minor base T (0/1); total (186/258)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 444 total aligned reads displayed.

GAAGACCGCGAAAGAGCATCAGAAGCAGGTAAAAAAGGTGGACAGCACAGCGGGGGTAATTTCAAGAATGACCCGCAGCGCGCATCTGAAGCAGGTAAAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCGACAACTAACCGGGCTAATCAATGACG‑AAT‑CATTTTTG‑‑CTGTAGCTCGTCAAAAAGCCATCACCGCCGGTTACCCGGTGGTTGATACTGATGACAAATGTAAGCTTGCCTGATGCGCGATGCTTATCAGGCCTACCAGAAGA  >  NZ_CP025268/1075221‑1075491

   gaCCGCGACAGAGCCTCAGAAGCAGGTACAAAAGGTGGACAGCACCGCGGGGGTAATTTCAAGAATGACCCGCAGCGCGCATCTGACGCAGGTAACATAGGTGGTAAGAGCAGTCACGGCAAAAGCGACAACTAGCCggg                                                                                                                                      <  2:8176391/140‑1 (MQ=255)

   gaCCGCGAAAGAGCATCAGAAGCAGGTAAAAAAGGTGGACAGCACAGCGGGGGTAATTTCAAGAATGACCCGCAGCGCGCATCTGAAGCAGGTAAAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCGACAACTAGCCggg                                                                                                                                      <  2:1139177/140‑1 (MQ=255)

   caCCGCGACAGAGCCTCAGAAGCAGGTAAAAACGGTGGACCGCACAGCGGGGGTAATTTCAAGAAAGACCCGCAGCGCGCATCTGACGCAGGTAAAAAAGGTGGTAAGAGCAGTCACGGCAAACGCGACAACTAGCCggg                                                                                                                                      <  2:6672320/139‑1 (MQ=255)

    aCCGCGAAAGATCATCAGAAGCAGGTAAAAAAGGTGGACAGCACAGCGGGGGTAATTTCAAGAATGACCCGCAGCGCGCATCTGAAGCAGGTAAAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCGACAACTAGCCGGGc                                                                                                                                     >  2:1032398/1‑140 (MQ=255)

      cgcgAAAGAGCATCAGAAGCAGGTAAAAAAGGTGGACAGCACAGCGGGGGTAATTTCAAGAATGACCCGCAGCGCGCATCTGAAGCAGGTAAAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCGACAACTAGCCGGGCtc                                                                                                                                   >  2:3146748/1‑139 (MQ=255)

      cgcgAAAGAGCATCAGAAGCAGGTAAAAAAGGTGGACAGCACAGCGGGGGTAATTTCAAGAATGACCCGCAGCGCGCATCTGAAGCAGGTAAAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCGACAACTAGCCGGGCTa                                                                                                                                   >  1:1687694/1‑140 (MQ=255)

      cgcgAAAGAGCATCAGAAGCAGGTAAAAAAGGTGGACAGCACAGCGGGGGTAATTTCAAGAATGACCCGCAGCGCGCATCTGAAGCAGGTAAAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCGACAACTAGCCGGGCTa                                                                                                                                   >  1:4574316/1‑140 (MQ=255)

      cgcgAAAGAGCATCAGAAGCAGGTAAAAAAGGTGGACAGCACAGCGGGGGTAATTTCAAGAATGACCCGCAGCGCGCATCTGAAGCAGGTAAAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCGACAACTAGCCGGGCTa                                                                                                                                   >  1:2726849/1‑140 (MQ=255)

                cATCAGAAGCAGGTAAAAAAGGTGGACAGCACAGCGGGGGTAATTTCAAGAATGACCCGCAGCGCGCATCTGAAGCAGGTAAAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCGACAACTAGCCGGGCTAATCAATGACg                                                                                                                         >  2:5884375/1‑140 (MQ=255)

                 aTCAGAAGCAGGTAAAAAAGGTGGACAGCACAGCGGGGGTAATTTCAAGAATGACCCGCAGCGCGCATCTGAAGCAGGTAAAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCGACAACTAGCCGGGCTAATCAATGACG‑a                                                                                                                       <  2:4721451/140‑1 (MQ=255)

                  tCAGAAGCAGGTAAAAAAGGTGGACAGCACAGCGGGGGTAATTTCAAGAATGACCCGCAGCGCGCATCTGAAGCAGGTAAAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCGACAACTAGCCGGGCTAATCACTGACG‑aa                                                                                                                      >  1:5362123/1‑140 (MQ=255)

                  tCAGAAGCAGGTAAAAAAGGTGGACAGCACAGCGGGGGTAATTTCAAGAATGACCCGCAGCGCGCATCTGAAGCAGGTAAAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCGACAACTAGCCGGGCTAATCAATGACG‑aa                                                                                                                      >  2:2799959/1‑140 (MQ=255)

                   cAGAAGCAGGTAAAAAAGGTGGACAGCACAGCGGGGGTAATTTCAAGAATGACCCGCAGCGCGCATCTGAAGCAGGTAAAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCGACAACTAGCCGGGCTAATCAATGACG‑AAt                                                                                                                     >  2:4319260/1‑140 (MQ=255)

                         cAGGTAAAAAAGGTGGACAGCACAGCGGGGGTAATTTCAAGAATGACCCGCAGCGCGCATCTGAAGCAGGTAAAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCGACAACTAGCCGGGCTAATCAATGACG‑AATGCAttt                                                                                                               <  1:2065031/140‑1 (MQ=255)

                         cAGGTAAAAAAGGTGGACAGCACAGCGGGGGTAATTTCAAGAATGACCCGCAGCGCGCATCTGAAGCAGGTAAAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCGACAACTAGCCGGGCTAATCAATGACG‑AATGCAttt                                                                                                               <  2:5127931/140‑1 (MQ=255)

                          aGGTAAAAAAGGTGGACAGCACAGCGGGGGTAATTTCAAGAATGACCCGCAGCGCGCATCTGAAGCAGGTACAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCGACAACTAGCCGGGCTAATCAATGACG‑AATGCAtttt                                                                                                              >  2:8959882/1‑140 (MQ=255)

                            gTAAAAAAGGTGGACAGCACAGCGGGGGTAATTTCAAGAATGACCCGCAGCGCGCATCTGAAGCAGGTAAAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCGACAACTAGCCGGGCTAATCAATGACG‑AATGCATTTTTg                                                                                                            >  2:1929656/1‑140 (MQ=255)

                                aaaaGGTGGACAGCACAGCGGGGGTAATTTCAAGAATGACCCGCAGCGCGCATCTGAAGCAGGTAAAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCGACAACTAGCCGGGCTAATCAATGACG‑AATGCATTTTTgtctg                                                                                                        >  1:3095723/1‑136 (MQ=255)

                                      tGGACAGCACAGCGGGGGTAATTTCAAGAATGACCCGCAGCGCGCATCTGAAGCAGGTAAAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCGACAACTAGCCGGGCTAATCAATGACG‑AATGCATTTTTGT‑CTGTAGCTc                                                                                                 >  2:6362283/1‑140 (MQ=255)

                                       ggACAGCACAGCGGGGGTAATTTCAAGAATGACCCGCAGCGCGCATCTGAAGCAGGTAAAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCGACAACTAGCCGGGCTAATCAATGACG‑AATGCATTTTTGT‑CTGTAGCTCg                                                                                                >  2:334280/1‑140 (MQ=255)

                                           agcacagcGGGGGTAATTTCAAGAATGACCCGCAGCGCGCATCTGAAGCAGGTAAAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCGACAACTAGCCGGGCTAATCAATGACG‑AATGCATTTTTGT‑CTGTAGCTCGTCaa                                                                                            <  2:5340413/140‑1 (MQ=255)

                                             cacagcGGGGGTAATTTCAAGAATGACCCGCAGCGCGCATCTGAAGCAGGTAAAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCGACAACTAGCCGGGCTAATCAATGACG‑AATGCATTTTTGT‑CTGTAGCTCGTCaaaa                                                                                          >  2:9233029/1‑140 (MQ=255)

                                             cacagcGGGGGTAATTTCAAGAATGACCCGCAGCGCGCATCTGAAGCAGGTAAAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCGACAACTAGCCGGGCTAATCAATGACG‑AATGCATTTTTGT‑CTGTAGCTCGTCaaaa                                                                                          >  1:7091357/1‑140 (MQ=255)

                                             cacagcGGGGGTAATTTCAAGAATGACCCGCAGCGCGCATCTGAAGCAGGTAAAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCGACAACTAGCCGGGCTAATCAATGACG‑AATGCATTTTTGT‑CTGTAGCTCGTCaaaa                                                                                          >  2:6120311/1‑140 (MQ=255)

                                              acagcGGGGGTAATTTCAAGAATGACCCGCAGCGCGCATCTGAAGCAGGTAAAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCGACAACTAGCCGGGCTAATCAATGACG‑AATGCATTTTTGT‑CTGTAGCTCGTCaaaaa                                                                                         <  2:4086800/140‑1 (MQ=255)

                                                agcGGGGGTAATTTCAAGAATGACCCGCAGCGCGCATCTGAAGCAGGTAAAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCGACAACTAGCCGGGCTAATCAATGACG‑AATGCATTTTTGT‑CTGTAGCTCGTCAAAAAGc                                                                                       >  2:362210/1‑140 (MQ=255)

                                                  cGGGGGTAATTTCAAGGATGACCCGCAGCGCGCATCTGAAGCAGGTAAAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCGACAACTAGCCGGGCTAATCAATGACG‑AATGCATTTTTGT‑CTGTAGCTCGTCAAAAAGCCa                                                                                     >  2:1452295/1‑140 (MQ=255)

                                                       gTAATTTCAAGAATGACCCGCAGCGCGCGTCTGAAGCAGGTAAAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCGACAACTAGCCGGGCTAAACAATGACG‑AATGCATTTTTGT‑CTGTAGCTCGTCAAAAAGCCATCAcc                                                                                <  1:7966400/140‑1 (MQ=255)

                                                       gTAATTTCAAGAATGACCCGCAGCGCGCATCTGAAGCAGGTAAAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCGACAACTAGCCGGGCTAATCAATGACG‑AATGCATTTTTGT‑CTGTAGCTCGTCAAAAAGCCATCAcc                                                                                >  2:421904/1‑140 (MQ=255)

                                                       gTAATTTCAAGAATGACCCGCAGCGCGCATCTGAAGCAGGTAAAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCGACAACTAGCCGGGCTAATCAATGACG‑AATGCATTTTTGT‑CTGTAGCTCGTCAAAAAGCCATCAcc                                                                                >  1:3925870/1‑140 (MQ=255)

                                                       gTAATTTCAAGAATGACCCGCAGCGCGCATCTGAAGCAGGTAAAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCGACAACTAGCCGGGCTAATCAATGACG‑AATGCATTTTTGT‑CTGTAGCTCGTCAAAAAGCCATCAcc                                                                                >  1:8746301/1‑140 (MQ=255)

                                                             tCAAGAATGACCCGCAGCGCGCATCTGAAGCAGGTAAAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCGACAACTAGCCGGGCTAATCAATGACG‑AATGCATTTTTGT‑CTGTAGCTCGTCAAAAAGCCATCACCGCCGGt                                                                          <  2:4545164/140‑1 (MQ=255)

                                                             gccaGAATTCCCCGCAGCTCCCATCTGAAGCCGGTAACAAAGGTGGTAAGAGCAGTCACGGCAAAAGCCACAACTCTCCGGGCTCATCCATGACG‑AATGCATTTTTGT‑CTGTAGCTCGTCAAAAAGCCATCACCGCCGGt                                                                          <  2:6415145/137‑1 (MQ=255)

                                                              cAAGAATGACCCGCAGCGCGCATCTGAAGCAGGTAAAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCGACAACTAGCCGGGCTAATCAATGACG‑AATGCATTTTTGT‑CTGTAGCTCGTCAAAAAGCCATCACCGCCGGtt                                                                         >  2:4970704/1‑140 (MQ=255)

                                                               aaGAATGACCCGCAGCGCGCATCTGAAGCAGGTAAAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCGACAACTAGCCGGGCTAATCAATGACG‑AATGCATTTTTGT‑CTGTAGCTCGTCAAAAAGCCATCACCGCCGGTTa                                                                        >  2:4659307/1‑140 (MQ=255)

                                                                 gAATGACCCGCAGCGCGCATCTGAAGCAGGTAAAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCGACAACTAGCCGGGCTAATCAATGACG‑AATGCATTTTTGT‑CTGTAGCTCGTCAAAAAGCCATCACCGCCGGTTAcc                                                                      <  1:8464646/140‑1 (MQ=255)

                                                                 gAATGACCCGCAGCGCGCATCTGAAGCAGGTAAAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCGACAACTAGCCGGGCTAATCAATGACG‑AATGCATTATTGT‑CTGTAGCTCGTCAAAAAGCCATCACCGCCGGTTAcc                                                                      <  2:8557786/140‑1 (MQ=255)

                                                                   aggACCCGCAGCGCGCATCTGAAGCAGGTAAAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCGACAACTAGCCGGGCTAATCAATGACG‑AATGCATTTTTGT‑CTGTAGCTCGTCAAAAAGCCATCACCGCCGGTTACCCg                                                                    <  2:8830782/138‑1 (MQ=255)

                                                                   aTGACCCGCAGCGCGCATCTGAAGCAGGTACAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCGACAACTAGCCGGGCTAATCAATGACG‑AATGCATTTTTGT‑CTGTAGCTCGTCAAAAAGCCATCACCGCCGGTTACCCg                                                                    <  2:2401116/140‑1 (MQ=255)

                                                                   aTGACCCGCAGCGCGCATCTGAAGCAGGTAAAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCGACAACTAGCCGGGCTAATCAATGACG‑AATGCATTTTTGT‑CTGTAGCTCGTCAAAAAGCCATCACCGCCGGTTACCCg                                                                    <  1:508948/140‑1 (MQ=255)

                                                                   aTGACCCGCAGCGCGCATCTGAAGCAGGTAAAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCGACAACTAGCCGGGCTAATCAATGACG‑AATGCATTTTTGT‑CTGTAGCTCGTCAAAAAGCCATCACCGCCGGTTACCCg                                                                    <  1:3509980/140‑1 (MQ=255)

                                                                   aTGACCCGCAGCGCGCATCTGAAGCAGGTAAAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCGACAACTAGCCGGGCTAATCAATGACG‑AATGCATTTTTGT‑CTGTAGCTCGTCAAAAAGCCATCACCGCCGGTTACCCg                                                                    <  2:2679487/140‑1 (MQ=255)

                                                                   aTGACCCGCAGCGCGCATCTGAAGCAGGTAAAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCGACAACTAGCCGGGCTAATCAATGACG‑AATGCATTTTTGT‑CTGTAGCTCGTCAAAAAGCCATCACCGCCGGTTACCCg                                                                    <  1:7371314/140‑1 (MQ=255)

                                                                   aTGACCCGCAGCGCGCATCTGAAGCAGGTAAAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCGACAACTAGCCGGGCTAATCAATGACG‑AATGCATTTTTGT‑CTGTAGCTCGTCAAAAAGCCATCACCGCCGGTTACCCg                                                                    <  2:5819514/140‑1 (MQ=255)

                                                                    tGACCCGCAGCGCGCATCTGAAGCAGGTAAAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCGACAACTAGCCGGGCTAATCAATGACG‑AATGCATTTTTGT‑CTGTAGCTCGTCAAAAAGCCATCACCGCCGGTTACCCgg                                                                   <  2:2054928/140‑1 (MQ=255)

                                                                      aCCCGCAGCGCGCATCTGAAGCAGGTAAAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCGACAACTAGCCGGGCTAATCAATGACG‑AATGCATTTTTGT‑CTGTAGCTCGTCAAAAAGCCATCACCGCCGGTTACCCggtg                                                                 >  2:6950664/1‑140 (MQ=255)

                                                                       cccGCAGCGCGCATCTGAAGCAGGTAAAAAAGGTGGTAAGAGCAGTCACGGCAACAGCGACACCTAGCCGGGCTAATCAATGACG‑AATGCATTTTTGT‑CTGTAGCTCGTCAAAAAGCCATCACCGCCGGTTACCCggtgg                                                                <  2:7782981/140‑1 (MQ=255)

                                                                        ccGCAGCGCGCATCTGAAGCAGGTAAAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCGACAACTAGCCGGGCTAATCAATGACG‑AATGCATTTTTGT‑CTGTAGCTCGTCAAAAAGCCATCACCGCCGGTTACCCggtggt                                                               <  2:7571578/140‑1 (MQ=255)

                                                                        ccGCAGCGCGCATCTGAAGCAGGTAAAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCGACAACTAGCCGGGCTAATCAATGACG‑AATGCATTTTTGT‑CTGTAGCTCGTCAAAAAGCCATCACCGCCGGTTACCCggtggt                                                               <  2:9299553/140‑1 (MQ=255)

                                                                           cAGCGCGCATCTGAAGCAGGTAAAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCGACAACTAGCCGGGCTAATCAATGACG‑AATGCATTTTTGT‑CTGTAGCTCGTCAAAAAGCCATCACCGCCGGTTACCCGGTGGTTGa                                                            <  2:3935209/140‑1 (MQ=255)

                                                                                cgcATCTGAAGCAGGTAAAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCGACAACTAGCCGGGCTAATCAATGACG‑AATGCATTTTTGT‑CTGTAGCTCGTCAAAAAGCCATCACCGCCGGTTACCCGGTGGTTGATACtg                                                       <  1:1266857/140‑1 (MQ=255)

                                                                                cgcATCTGAAGCAGGTAAAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCGACAACTAGCCGGGCTAATCAATGACG‑AATGCATTTTTGT‑CTGTAGCTCGTCAAAAAGCCATCACCGCCGGTTACCCGGTGGTTGATACtg                                                       >  2:596100/1‑140 (MQ=255)

                                                                                cgcATCTGAAGCAGGTAAAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCGACAACTAGCCGGGCTAATCAATGACG‑AATGCATTTTTGT‑CTGTAGCTCGTCAAAAAGCCATCACCGCCGGTTACCCGGTGGTTGATACtg                                                       >  2:3383520/1‑140 (MQ=255)

                                                                                 gcATCTGAAGCAGGTAAAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCGACAACTAGCCGGGCTAATCAATGACG‑AATGCATTTTTGT‑CTGTAGCTCGTCAAAAAGCCATCACCGCCGGTTACCCGGTGGTTGATACtga                                                      <  1:6658464/140‑1 (MQ=255)

                                                                                   aTCTGAAGCAGGTAAAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCGACAACTAGCCGGGCTAATCAATGACG‑AATGCATTTTTGT‑CTGTAGCTCGTCAAAAAGCCATCACCGCCGGTTACCCGGTGGTTGATACtgatg                                                    >  1:4078145/1‑140 (MQ=255)

                                                                                   aTCTGAAGCAGGTAAAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCGACAACTAGCCGGGCTAATCAATGACG‑AATGCATTTTTGT‑CTGTAGCTCGTCAAAAAGCCATCACCGCCGGTTACCCGGTGGTTGATACtgatg                                                    >  2:2330721/1‑140 (MQ=255)

                                                                                    tCTGAAGCAGGTAAAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCGACAACTAGCCGGGCTAATCAATGACG‑AATGCATTTTTGT‑CTGTAGCTCGTCAAAAAGCCATCACCGCCGGTTACCCGGTGGTTGATACtgatga                                                   <  2:2902270/140‑1 (MQ=255)

                                                                                     cTGAAGCAGGTAAAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCGACAACTAGCCGGGCTAATCAATGACG‑AATGCATTTTTGT‑CTGTAGCTCGTCAAAAAGCCATCACCGCCGGTTACCCGGTGGTTGATACTGATGAc                                                  <  1:9354516/140‑1 (MQ=255)

                                                                                     cTGAAGCAGGTAAAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCGACAACTAGCCGGGCTAATCAATGACG‑AATGCATTTTTGT‑CTGTAGCTCGTCAAAAAGCCATCACCGCCGGTTACCCGGTGGTTGATACTGATGAc                                                  <  1:2411776/140‑1 (MQ=255)

                                                                                     cTGAAGCAGGTAAAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCGACAACTAGCCGGGCTAATCAATGACG‑AATGCATTTTTGT‑CTGTAGCTCGTCAAAAAGCCATCACCGCCGGTTACCCGGTGGTTGATACTGATGAc                                                  <  1:8689377/140‑1 (MQ=255)

                                                                                        aaGCAGGTAAAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCGACAACTAGCCGGGCTAATCAATGACG‑AATGCATTTTTGT‑CTGTAGCTCGTCAAAAAGCCATCACCGCCGGTTACCCGGTGGTTGATACTGATGACaaa                                               >  1:1006613/1‑140 (MQ=255)

                                                                                             ggTAAAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCGACAACTAGCCGGGCTAATCAATGACG‑AATGCATTTTTGT‑CTGTAGCTCGTCAAAAAGCCATCACCGCCGGTTACCCGGTGGTTGATACTGATGCCAAATGTaa                                          >  1:6329341/1‑140 (MQ=255)

                                                                                             ggTAAAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCGACAACTAGCCGGGCTAATCAATGACG‑AATGCATTTTTGT‑CTGTAGCTCGTCAAAAAGCCATCACCGCCGGTTACCCGGTGGTTGATACTGATGACAAATGTaa                                          >  2:8453982/1‑140 (MQ=255)

                                                                                             ggTAAAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCGACAACTAGCCGGGCTAATCAATGACG‑AATGCATTTTTGT‑CTGTAGCTCGTCAAAAAGCCATCACCGCCGGTTACCCGGTGGTTGATACTGATGACAAATGTaa                                          >  1:4733802/1‑140 (MQ=255)

                                                                                             ggTAAAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCGACAACTAGCCGGGCTAATCAATGACG‑AATGCATTTTTGT‑CTGTAGCTCGTCAAAAAGCCATCACCGCCGGTTACCCGGTGGTTGATACTGATGACAAATGTaa                                          >  1:6078512/1‑140 (MQ=255)

                                                                                             ggTAAAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCGACAACTAGCCGGGCTAATCAATGACG‑AATGCATTTTTGT‑CTGTAGCTCGTCAAAAAGCCATCACCGCCGGTTACCCGGTGGTTGATACTGAAGACcaaaggaa                                          >  1:91001/1‑135 (MQ=255)

                                                                                              gTAAAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCGACAACTAGCCGGGCTAATCAATGACG‑AATGCATTTTTGT‑CTGTAGCTCGTCAAAAAGCCATCACCGCCGGTTACCCGGTGGTTGATACTGATGACAAATGTAAg                                         <  1:6950664/140‑1 (MQ=255)

                                                                                              gTAAAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCGACAACTAGCCGGGCTAATCAATGACG‑AATGCATTTTTGT‑CTGTAGCTCGTCAAAAAGCCATCACCGCCGGTTACCCGGTGGTTGATACTGATGACAAATGTAAg                                         <  1:1543447/140‑1 (MQ=255)

                                                                                              gTAAAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCGACAACTAGCCGGGCTAATCAATGACG‑AATGCATTTTTGT‑CTGTAGCTCGTCAAAAAGCCATCACCGCCGGTTACCCGGTGGTTGATACTGATGACAAATGTAAg                                         >  1:7260181/1‑140 (MQ=255)

                                                                                               tAAAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCGACAACTAGCCGGGCTAATCAATGACG‑AATGCATTTTTGT‑CTGTAGCTCGTCAAAAAGCCATCACCGCCGGTTACCCGGTGGTTGATACTGATGACAAATGTAAGc                                        >  1:4855208/1‑140 (MQ=255)

                                                                                                      ggtggtAAGAGCAGTCACGGCAAAAGCGACAACTAGCCGGGCTAATCAATGACG‑AATGCATTTTTGT‑CTGTAGCTCGTCAAAAAGCCATCACCGCCGGTTACCCGGTGGTTGATACTGATGACAAATGTAAGCTTGCCTg                                 <  1:9489279/140‑1 (MQ=255)

                                                                                                        tggtAAGAGCAGTCACGGCAAAAGCGACAACGAGCCGGGCTAATCAATGACGCCGG‑CATTTGTGT‑CTGTAGCTCGTCAAACAGCCATCACCGCCGGTTACCCGGTGGTTGATACTGATGACAAATTTCAGCTTGCCTGAt                               <  2:8349088/140‑1 (MQ=255)

                                                                                                         ggtAAGAGCAGTCACGGCAACAGCGACAACTAGCCGGGCTAATCAATGACG‑AATGCATTTTTGT‑CTGTAGCTCGTCAAAAAGCCATCACCGCCGGTTACCCGGTGGTTGATACTGATGACAAATGTAAGCTTGCCTGATg                              <  2:1739866/140‑1 (MQ=255)

                                                                                                         ggtAAGAGCAGTCACGGCAAAAGCGACAACTAGCCGGGCGAATCAATGACG‑AATGCATTTTTGT‑CTGTAGCTCGTCAAAAAGCCATCACCGCCGGTTACCCGGTGGGTGATACTGATGAGAAATGTAGGCTTGCCTGATg                              >  1:6757417/1‑140 (MQ=255)

                                                                                                           tAAGAGCAGTCACGGCAAAAGCGACANCTAGCCGGGCTAATCAATGACG‑AATGCATTTTTGT‑CTGTAGCTCGTCAAAAAGCCATCACCGCCGGTTACCCGGTGGTTGATACTGATGACAAATGTAANCTTGCCTGATgcg                            <  1:5534950/140‑1 (MQ=255)

                                                                                                           tAAGAGCAGTCACGGCAAAAGCGACAACTCGCCGGGCTAATCAATGACG‑AATGCATTTTTGT‑CTGTAGCTCGTCAAAAAGCCATCACCGCCGGTTACCCGGTGGTTGATACTGATGACAAATGTAAGCTTGCCTGATgcg                            <  2:3020619/140‑1 (MQ=255)

                                                                                                           tAAGAGCAGTCACGGCAAAAGCGACAACTCGCCGGGCTAAACAATGACG‑ACT‑GAATTTTGGTCTGTAGCTCGTCAAAAAGCCATCACCGCCGGTTACCCGGTGGTTGATACTGATGACAAATGTAAGCTTGCCTGATgcg                            <  1:3825149/140‑1 (MQ=255)

                                                                                                           tAAGAGCAGTCACGGCAAAAGCGACAACTAGCCGGGCTAATCAATGACG‑AATGCATTTTTGT‑CTGTAGCTCGTCAAAAAGCCATCACCGCCGGTTACCCGGTGGTTGATACTGATGACAAATGTAAGCTTGCCTGATgcg                            <  1:720687/140‑1 (MQ=255)

                                                                                                           tAAGAGCAGTCACGGCAAAAGCGACAACTAGCCGGGCTAATCAATGACG‑AATGCATTTTTGT‑CTGTAGCTCGTCAAAAAGCCATCACCGCCGGTTACCCGGTGGTTGATACTGATGACAAATGTAAGCTTGCCTGATgcg                            <  1:5277137/140‑1 (MQ=255)

                                                                                                           tAAGAGCAGTCACGGCAAAAGCGACAACTAGCCGGGCTAATCAATGACG‑AATGCATTTTTGT‑CTGTAGCTCGTCAAAAAGCCATCACCGCCGGTTACCCGGTGGTTGATACTGATGACAAATGTAAGCTTGCCTGATgcg                            <  1:4178014/140‑1 (MQ=255)

                                                                                                           tAAGAGCAGTCACGGCAAAAGCGACAACTAGCCGGGCTAATCAATGACG‑AATGCATTTTTGT‑CTGTAGCTCGTCAAAAAGCCATCACCGCCGGTTACCCGGTGGTTGATACTGATGACAAATGTAAGCTTGCCTGATgcg                            <  2:3748637/140‑1 (MQ=255)

                                                                                                            aaGAGCAGTCACGGCAAAAGCGACAACTAGCCGGGCTAATCAATGACG‑AATGCATTTTTGT‑CTGTAGCTCGTCAAAAAGCCATCACCGCCGGTTACCCGGTGGTTGATACTGATGACAAATGTAAGCTTGCCTGATgcgc                           >  1:651880/1‑140 (MQ=255)

                                                                                                              gagCAGTCACGGCAAAAGCGACAACTAGCCGGGCTACTCAATGACG‑AATGCATTTTTGT‑CTGGAGCTCGTCAAAAAGCCATCACCGCCGGTTACCCGGTGGTTGATACTGATGACAAATGTAAGCTTGCCTGATGCGCGa                         <  1:2245632/140‑1 (MQ=255)

                                                                                                              gagCAGTCACGGCAAAAGCGACAACTAGCCGGGCTAATCAATGACG‑AATGCATTTTTGT‑CTGTAGCTCGTCAAAAAGCCATCACCGCCGGTTACCCGGTGGTTGATACTGATGACAAATGTAAGCTTGCCTGATGCGCGa                         <  1:1188528/140‑1 (MQ=255)

                                                                                                              gagCAGTCACGGCAAAAGCGACAACTAGCCGGGCTAATCAATGACG‑AATGCATTTTTGT‑CTGTAGCTCGTCAAAAAGCCATCACCGCCGGTTACCCGGTGGTTGATACTGATGACAAATGTAAGCTTGCCTGATGCGCGa                         <  2:5201386/140‑1 (MQ=255)

                                                                                                               agCAGTCACGGCAAAAGCGACAACTAGCCGGGCTAATCAATGACG‑AATGCATTTTTGT‑CTGTAGCTCGTCAAAAAGCCATCACCGCCGGTTACCCGGTGGTTGATACTGATGACAAATGTAAGCTTGCCTGATGCGCGAt                        <  1:4936893/140‑1 (MQ=255)

                                                                                                                gCAGTCACGGCAAAAGCGACAACTAGCCGGGCTAATCAATGACG‑AATGCATTTTTGT‑CTGTAGCTCGTCAAAAAGCCATCACCGCCGGTTACCCGGTGGTTGATACTGATGACAAATGTAAGCTTGCCTGATGCGCGATg                       <  1:4373514/140‑1 (MQ=255)

                                                                                                                  aGTCACGGCAAAAGCGACAACTAGCCGGGCTAATCAATGACG‑AATGCATTTTTGT‑CTGTAGCTCGTCAAAAAGCCATCACCGCCGGTTACCCGGTGGTTGATACTGATGACAAATGTAAGCTTGCCTGATGCGCGATGCt                     >  1:6163219/1‑140 (MQ=255)

                                                                                                                    tCACGGCAAAAGCGACAACTAGCCGGGCTAATCAATGACG‑AATGCATTTTTGT‑CTGTAGCTCGTCAAAAAGCCATCACCGCCGGTTACCCGGTGGTTGATACTGATGACAAATGTAAGCTTGCCTGATGCGCGATGCTTa                   >  1:2923911/1‑140 (MQ=255)

                                                                                                                      aCGGCAAAAGCGACAACTAGCCGGGCTAATCAATGACG‑AATGCATTTTTGT‑CTGTCGCTCGTCAAAAAGCCATCACCGCCGGTGACCCGGTGGTTGATACTGATGACAAATGTAAGCTTGCCTGATGCGCGATGCTTATc                 <  2:796828/140‑1 (MQ=255)

                                                                                                                       cGGCAAAAGCGACAACTAGCCGGGCTAATCAATGACG‑AATGCATTTTTGT‑CTGTAGCTCGTCAAAAAGCCATCACCGCCGGTTACCCGGTGGTTGATACTGATGACAAATGTAAGCTTGCCTGATGCGCGATGCTTATCa                <  2:5611985/140‑1 (MQ=255)

                                                                                                                         gCAAAAGCGACAACTAGCCGGGCTAATCAATGACG‑AATGCATTTTTGT‑CTGTAGCTCGTCAAAAAGCCATCACCGCCGGTTACCCGGTGGTTGATACTGATGACAAATGTAAGCTTGCCTGATGCGCGATGCTTATCAgg              <  1:8991803/140‑1 (MQ=255)

                                                                                                                         gCAAAAGCGACAACTAGCCGGGCTAATCAATGACG‑AATGCATTTTTGT‑CTGTAGCTCGTCAAAAAGCCATCACCGCCGGTTACCCGGTGGTTGATACTGATGACAAATGTAAGCTTGCCTGATGCGCGATGCTTATCAgg              >  1:7574694/1‑140 (MQ=255)

                                                                                                                         gCAAAAGCGACAACTAGCCGGGCTAATCAATGACG‑AATGCATTTTTGT‑CTGTAGCTCGTCAAAAAGCCATCACCGCCGGTTACCCGGTGGTTGATACTGATGACAAATGTAAGCTTGCCTGATGCGCGATGCTTATCAgg              <  1:2607474/140‑1 (MQ=255)

                                                                                                                              aGCGACAACTAGCCGGGCTAATCAATGACG‑AATGCATTTTTGT‑CTGTAGCTCGTCAAAAAGCCATCACCGCCGGTTACCCGGTGGTTGATACTGATGACAATTGTAAGCTTGCCTGATGCGCGATGCTTATCAGGCCTAc         >  1:9042574/1‑140 (MQ=255)

                                                                                                                                   cAACTAGCCGGGCTAATCAATGACG‑AATGCATTTTTGT‑CTGTAGCTCGTCAAAAAGCCATCACCGCCGGTTACCCGGTGGTTGATACTGATGACATATGTAAGCTTGCCTGATGCGCGATGCTTATCAGGCCTACCagaa    <  2:616939/140‑1 (MQ=255)

                                                                                                                                   cAACTAGCCGGGCTAATCAATGACG‑AATGCATTTTTGT‑CTGTAGCTCGTCAAAAAGCCATCACCGCCGGTTACCCGGTGGTTGATACTGATGACAAATGTAAGCTTGCCTGATGCGCGATGCTTATCAGGCCTACCagaa    <  1:5884375/140‑1 (MQ=255)

                                                                                                                                   cAACTAGCCGGGCTAATCAATGACG‑AATGCATTTTTGT‑CTGTAGCTCGTCAAAAAGCCATCACCGCCGGTTACCCGGTGGTTGATACTGATGACAAATGTAAGCTTGCCTGATGCGCGATGCTTATCAGGCCTACCagaa    <  1:5638593/140‑1 (MQ=255)

                                                                                                                                   cAACTAGCCGGGCTAATCAATGACG‑AATGCATTTTTGT‑CTGTAGCTCGTCAAAAAGCCATCACCGCCGGTTACCCGGTGGTTGATACTGATGACAAATGTAAGCTTGCCTGATGCGCGATGCTTATCAGGCCTACCagaa    <  2:3095723/140‑1 (MQ=255)

                                                                                                                                     aCTAGCCGGGCTAATCAATGACG‑AATGCATTTTTGT‑CTGTAGCTCGTCAAAAAGCCATCACCGCCGGTTACACGGTGGTTGATTCTGATGACAAATGTAATCTTGCCTGATGCGCGATGCTTATCAGGCCTACCagaaga  <  2:7257938/140‑1 (MQ=255)

GAAGACCGCGAAAGAGCATCAGAAGCAGGTAAAAAAGGTGGACAGCACAGCGGGGGTAATTTCAAGAATGACCCGCAGCGCGCATCTGAAGCAGGTAAAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCGACAACTAACCGGGCTAATCAATGACG‑AAT‑CATTTTTG‑‑CTGTAGCTCGTCAAAAAGCCATCACCGCCGGTTACCCGGTGGTTGATACTGATGACAAATGTAAGCTTGCCTGATGCGCGATGCTTATCAGGCCTACCAGAAGA  >  NZ_CP025268/1075221‑1075491

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`