BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP025268	1,207,982	A→G	V51A (GTC→GCC)	xisE ←	excisionase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP025268	1,207,982	0	A	G	99.7%	2362.5 / NA	700	V51A (GTC→GCC)	xisE	excisionase
Reads supporting (aligned to +/- strand): ref base A (0/0); major base G (325/372); minor base C (1/1); total (327/373)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 700 total aligned reads displayed.

CCTACTATAAAGCGGATATAAGTTAGGGACTGAAACATTATTCTTACGCGGTCTGGCTGCCATTACTCAAAATCCGTTGCAAAAGTAATGAGTCATTTTTCTTGATTACAGGTGTTACCAACTCCCCAACTAACTCGACGTCCTCACGCACTCGCCATAACCGGCCTTGTTTCATGGCCGGTGGACAAAATAAATTCTGCTTAGCATAACGACGCAATGTGGACACACTTGGAGGATTACTTCTGTATTTTTCAGCAGCCCATTCTTCAAGAGT  >  NZ_CP025268/1207845‑1208118

ccTACTATAAAGCGGATATAAGTTAGGGACTGAAACATTATTCTTACGCGGTCTGGCTGCCATTACTCAAAATCCGTTGCAATAGTAATGAGTCATTTTTCTTGATTACAGGTGTTACCAACTCCCCAACTAACTCGGCg                                                                                                                                        >  2:8552647/1‑140 (MQ=255)

ccTACTATAAAGCGGATATAAGTTAGGGACTGAAACATTATTCTTACGCGGTCTGGCTGCCATTACTCAAAATCCGTTGCAAAAGTAATGAGTCATTTTTCTTGATTACAGGTGTTACCAACTCCCCAACTAACTCGGCg                                                                                                                                        >  1:6612909/1‑140 (MQ=255)

ccTACTATAAAGCGGATATAAGTTAGGGACTGAAACATTATTCTTACGCGGTCTGGCTGCCATTACTCAAAATCCGTTGCAAAAGTAATGAGTCATTTTTCTTGATTACAGGTGTTACCAACTCCCCAACTAACTCGGCg                                                                                                                                        >  1:3376756/1‑140 (MQ=255)

      ataCAGCGGATATAAGTTAGGGACTGAAACATTATTCTTACGCGGTCTGGCTGCCATTACTCAAAATCCGTTGCAAAAGTAATGAGTCATTTTTCTTGATTACAGGTGTTACCAACTCCCCAACTAACTCGGCGTCCTCa                                                                                                                                  <  2:1875179/140‑1 (MQ=255)

        aaaGCGGATATAAGTTAGGGACTGAAACATTATTCTTACGCGGTCTGGCTGCCATTACTCAAAATCCGTTGCAAAAGTAATGAGTCATTTTTCTTGATTACAGGTGTTACCAACTCCCCAACTAACTCGGCGTCCTCACg                                                                                                                                <  2:1037903/140‑1 (MQ=255)

          aGCGGATATCAGTTAGGGACTGAAACATTATTCTTACGCGGTCTGGCTGCCATTACTCAACATCCGTTGCAAAAGTAATGAGTCATTTTTCTTGATTACAGGTGTTACCAACTCCCCAACTAACTCGGCGTCCTCACGCa                                                                                                                              <  1:5470297/140‑1 (MQ=255)

          aGCGGATATAAGTTAGGGACTGAAACATTATTCTTACGCGGTCTGGCTGCCATTACTCAAAATCCGTTGCAAAAGTAATGAGTCATTTTTCTTGATTACAGGTGTTACCAACTCCCCAACTAACTCGGCGTCCTCACGCa                                                                                                                              >  2:9400624/1‑140 (MQ=255)

          aGCGGATATAAGTTAGGGACTGAAACATTATTCTTACGCGGTCTGGCTGCCATTACTCAAAATCCGTTGCAAAAGTAATGAGTCATTTTTCTTGATTACAGGTGTTACCAACTCCCCAACTAACTCGGCGTCCTCACGCa                                                                                                                              <  1:5327371/140‑1 (MQ=255)

          aGCGGATATAAGTTAGGGACTGAAACATTATTCTTACGCGGTCTGGCTGCCATTACTCAAAATCCGTTGCAAAAGTAATGAGTCATTTTTCTTGATTACAGGTGTTACCAACTCCCCAACTAACTCGGCGTCCTCACGCa                                                                                                                              <  2:4518450/140‑1 (MQ=255)

          aGCGGATATAAGTTAGGGACTGAAACATTATTCTTACGCGGTCTGGCTGCCATTACTCAAAATCCGTTGCAAAAGTAATGAGTCATTTTTCTTGATTACAGGTGTTACCAACTCCCCAACTAACTCGGCGTCCTCACGCa                                                                                                                              >  2:4012413/1‑140 (MQ=255)

          aGCGGATATAAGTTAGGGACTGAAACATTATTCTTACGCGGTCTGGCTGCCATTACTCAAAATCCGTTGCAAAAGTAATGAGTCATTTTTCTTGATTACAGGTGTTACCAACTCCCCAACTAACTCGGCGTCCTCACGCa                                                                                                                              >  2:9183552/1‑140 (MQ=255)

          aGCGGATATAAGTTAGGGACTGAAACATTATTCTTACGCGGTCTGGCTGCCATGACTCAAAATCCGTTGCCAAAGTAATGAGTCATTTTTCTTGATTACAGGTGTTACCAACTCCCCAACTAACTCGGCGTCCTCACGCa                                                                                                                              <  1:1866932/140‑1 (MQ=255)

          aGCGGATATAAGTTAGGGACTGAAACATTATTCTTACGCGGTCTGGCTGCCAGTACTCAAAATCCGTTGCAAAAGTAATGAGTCATTTTTCTTGATTACAGGTGTTACCAACTCCCCAACTAACTCGGCGTCCTCACGCa                                                                                                                              <  2:2320317/140‑1 (MQ=255)

          aGCGGATATAAGGTAGGGACTGAAACATTATTCTTACGCGGTCTGGCTGCCATTACTCAAAATCCGTTGCAAAAGTAATGAGTCAGTTTTCTTGATTACAGGTGTTACCAACTCCCCAACTAACTCGGCGTCCTCACGCa                                                                                                                              >  1:164423/1‑140 (MQ=255)

           gCGGATATAAGTTAGGGACTGAAACATTATTCTTACGCGGTCTGGCTGCCATTACTCAAAATCCGTTGCAAAAGTAATGAGTCATTTTTCTTGATTACAGGTGTTACCAACTCCCCAACTAACTCGGCGTCCTCTCGCAc                                                                                                                             >  2:5877625/1‑140 (MQ=255)

           gCGGATATAAGTTAGGGACTGAAACATTATTCTTACGCGGTCTGGCTGCCATTACTCAAAATCCGTTGCAAAAGTAATGAGTCATTTTTCTTGATTACAGGTGTTACCAACTCCCCAACTAACTCGGCGTCCTCACGCAc                                                                                                                             <  2:6201961/140‑1 (MQ=255)

            cGGATATAAGTTAGGGACTGAAACATTATTCTTACGCGGTCTGGCTGCCATTACTCAAAATCCGTTGCAAAAGTAATGAGTCATTTTTCTTGTTTACAGGTGTTACCAACTCCCCAACTAACTCGGCGTCCTCACGCACt                                                                                                                            >  1:7923920/1‑140 (MQ=255)

            cGGATATAAGTTAGGGACTGAAACATTATTCTTACGCGGTCTGGCTGCCATTACTCAAAATCCGTTGCAAAAGTAATGAGTCATTTTTCTTGATTACAGGTGTTACCAACTCCCCAACTAACTCGGCGTCCTCACGCACt                                                                                                                            <  2:7894820/140‑1 (MQ=255)

              gATATAAGTTAGGGACTGAAACATTATTCTTACGCGGTCTGGCTGCCATTACTCAAAATCCGTTGCAAAAGTAATGAGTCATTTTTCTTGATTACAGGTGTTACCAACTCCCCAACTAACTCGGCGTCCTCACGCACTCg                                                                                                                          >  2:374216/1‑140 (MQ=255)

              gATATAAGTTAGGGACTGAAACATTATTCTTACGCGGTCTGGCTGCCATTACTCAAAATCCGTTGCAAAAGTAATGAGTCATTTTTCTTGATTACAGGTGTTACCAACTCCCCAACTAACTCGGCGTCCTCACGCACTCg                                                                                                                          >  1:5755572/1‑140 (MQ=255)

               atatAAGTTAGGGACTGAAACATTATTCTTACGCGGTCTGGCTGCCATTACTCAAAATCCGTTGCAAAAGTAATGAGTCATTTTTCTTGATTACAGGTGTTACCAACTCCCCAACTAACTCGGCGTCCTCACGCACTCGc                                                                                                                         >  1:2678343/1‑140 (MQ=255)

               atatAAGTTAGGGACTGAAACATTATTCTTACGCGGTCTGGCTGCCATTACTCAAAATCCGTTGCAAAAGTAATGAGTCATTTTTCTTGATTACAGGTGTTACCAACTCCCCAACTAACTCGGCGTCCTCACGCACTCGc                                                                                                                         >  1:7715592/1‑140 (MQ=255)

               atatAAGTTAGGGACTGAAACATTAGTCTTACGCGGTCTGGCTGCCATTACTCAAAATCCGTTGCAAAAGTTATGAGTCATTTAACTGGATTACAGGTGTTACCACCTCCCCAACTAACTCGGCGTCCTCTCGCACTCGc                                                                                                                         >  1:9491133/1‑140 (MQ=255)

                 ataAGTTAGGGACTGAAACATTATTCTTACGCGGTCTGGCTGCCATTACTCAAAATCCGTTGCAAAAGTAATGAGTCATTTTTCTTGATTACAGGTGTTACCAACTCCCCAACTAACTCGGCGTCCTCACGCACTCGCCa                                                                                                                       >  2:66969/1‑140 (MQ=255)

                 ataAGTTAGGGACTGAAACATTATTCTTACGCGGTCTGGCTGCCATTACTCAAAATCCGTTGCAAAAGTAATGAGTCATTTTTCTTGATTACAGGTGTTACCAACTCCCCAACTAACTCGGCGTCCTCACGCACTCGCCa                                                                                                                       <  1:5155555/140‑1 (MQ=255)

                     gTTAGGGACTGAAACATTATTCTTACGCGGTCTGGCTGCCATTACTCAAAATCCGTTGCAAAAGTAATGAGTCATTTTTCTTGATTACAGGTGTTACCAACTCCCCAACTAACTCGGCGTCCTCACGCACTCGCCATAAc                                                                                                                   <  2:6044802/140‑1 (MQ=255)

                     gTTAGGGACTGAAACATTATTCTTACGCGGTCTGGCTGCCATTACTCAAAATCCGTTGCAAAAGTAATGAGTCATTTTTCTTGATTACAGGTGTTACCAACTCCCCAACTAACTCGGCGTCCTCACGCACTCGCCATAAc                                                                                                                   <  1:2061885/140‑1 (MQ=255)

                        aGGGACTGAAACATTATTCTTACGCGGTCTGGCTGCCATTACTCAAAATCCGTTGCAAAAGTAATGAGTCATTTTTCTTGATTACAGGTGTTACCAACTCCCCAACTAACTCGGCGTCCTCACGCACTCGCCATAACCgg                                                                                                                <  1:7082083/140‑1 (MQ=255)

                         gggACTGAAACCTTATCCTAACGCGGTCTGGCAGCCATTACTCAAAATCCGTTGCAAAAGTAATGAGTCATTTTTCGTGATTACAGGTGTTACCAACTCCCCAACTAACTCGGCGTCCTCACGCACTCGCCATAACCGGc                                                                                                               >  1:3850549/1‑140 (MQ=255)

                         gggACTGAAACATTATTCTTACGCGGTCTGGCTGCCATTACTCAAAATCCGTTGCAAAAGTAATGAGTCATTTTTCTTGATTACAGGTGTTACCAACTCCCCAACTAACTCGGCg                                                                                                                                        >  2:3138455/1‑115 (MQ=255)

                         gggACTGAAACATTATTCTTACGCGGTCTGGCTGCCATTACTCAAAATCCGTTGCAAAAGTAATGAGTCATTTTTCTTGATTACAGGTGTTACCAACTCCCCAACTAACTCGGCGTCCTCACGCACTCGCCATAACCGGc                                                                                                               >  2:4184536/1‑140 (MQ=255)

                         gggACTGAAACATTATTCTTACGCGGTCTGGCTGCCATTACTCAAAATCCGTTGCAAAAGTAATGAGTCATTTTTCTTGATTACAGGTGTTACCAACTCCCCAACTAACTCGGCGTCCTCACGCACTCGCCATAACCGGc                                                                                                               >  2:5731076/1‑140 (MQ=255)

                         gggACAGAAACCTTATTCATACGCGGTCTGGCTGCCATAACTCAAAATCCGTTGCAAAAGTAAAGAGTCATTTATCTTGATTACAGGTGTAACCAACACCCCAACTAACTCGGCGTCATCACGCTCTCGCCATAACCCGc                                                                                                               >  2:1529283/1‑140 (MQ=255)

                          ggACTGAAACATTATTCTTACGCGGTCTGGCTGCCATTACTCAAAATCCGTTGCAAAAGTAATGAGTCATTTTTCTTGATTACAGGTGTTACCAACTCCCCAACTAACTCGGCGTCCTCACGCACTCGCCATAACCGGcc                                                                                                              <  2:5022151/140‑1 (MQ=255)

                                aaaCATTATTCTTACGCGGTCTGGCTGCCATTACTCAAAATCCGTTGCAAAAGTAATGAGTCATTTTTCTTGATTACAGGTGTTACCAACTCCCCAACTAACTCGGCGTCCTCACGCACTCGCCATAACCGGCCTTGttt                                                                                                        >  1:2517694/1‑140 (MQ=255)

                                aaaCATTATTCTTACGCGGTCTGGCTGCCATTACTCAAAATCCGTTGCAAAAGTAATGAGTCATTTTTCTTGATTACAGGTGTTACCAACTCCCCAACTAACTCGGCGTCCTCACGCACTCGCCATAACCGGCCTTGttt                                                                                                        >  2:8732401/1‑140 (MQ=255)

                                      tattCTTACGCGGTCTGGCTGCCATTACTCAAAATCCGTTGCAAAAGTAATGAGTCATTTTTCTTGATTACAGTTGTTACCAACTCCCCAACTAACTCGGCGTCCTCACTCACTCGCCATAACCGGCCTTGTTTCATGGc                                                                                                  >  2:8037215/1‑140 (MQ=255)

                                      tattCTTACGCGGTCTGGCTGCCATTACTCAAAATCCGTTGCAAAAGTAATGAGTCATTTTTCTTGATTACAGGTGTTACCAACTCCCCAACTAACTCGGCGTCCTCACGCACTCGCCATAACCGGCCTTGTTTCATGGc                                                                                                  <  2:5360295/140‑1 (MQ=255)

                                             aCGCGGTCTGGCTGCCATTACTCAAAATCCGTTGCAAAAGTAATGAGTCATTTTTCTTGATTACAGGTGTTACCAACTCCCCAACTAACTCGGCGTCCTCACGCACTCGCCATAACCGGCCTTGTTTCATGGCCGGTGGa                                                                                           >  2:1656302/1‑140 (MQ=255)

                                             aCGCGGTCTGGCTGCCATTACTCAAAATCCGTTGCAAAAGTAATGAGTCATTTTTCTTGATTACAGGTGTTACCAACTCCCCAACTAACTCGGCGTCCTCACGCACTCGACATAACCGGCCTTGTTTCATGGCCGGTGGa                                                                                           >  2:744991/1‑140 (MQ=255)

                                             aCGCGGTCTGGCTGCCATTACTCAAAATCCGTTGCAAAAGTAATGAGTCATTTTTCTTGATTACAGGTGGTACCAACTCCCCAACTAACTCGGCGTCCTCACGCACTCGCCATAACCGGCCTTGTTTCATGGCCGGTGGa                                                                                           <  2:5568137/140‑1 (MQ=255)

                                              cgcgGTCTGGCTGCCATTACTCATAATCCGTTGCAAAAGTAATTAGTCATTTTTCTTGATTACAGGTGTTACCAACTCCCCAACTAACTCGGCGTCCTCACGCACTCGCCATAACCGGCCTTGTTTCATGGCCGGTGGAc                                                                                          <  2:6450123/140‑1 (MQ=255)

                                              cgcgGTCTGGCTGCCATTACTCAAAATCCGTTGCAAAAGTAATGAGTCATTTTTCTTGATTACAGGTGTTACCAACTCCCCNACTAACTCGGCGTCCTCACGCACTCGCCATAACCGGCCTTGTTTCATGGNCGGTGGAc                                                                                          <  1:5600708/140‑1 (MQ=255)

                                              cgcgGTCTGGCTGCCATTACTCAAAATCCGTTGCAAAAGTAATGAGTCATTTTTCTTGATTACAGGTGTTACCAACTCCCCAACTAACTCGGCGTCCTCACGCACTCGCCATAACCGGCCTTGTTTCATGGCCGGTGGAc                                                                                          <  2:2790887/140‑1 (MQ=255)

                                              cgcgGTCTGGCTGCCATTACTCAAAATCCGTTGCAAAAGTAATGAGTCATTTTTCTTGATTACAGGTGTTACCAACTCCCCAACTAACTCGGCGTCCTCACGCACTCGCCATAACCGGCCTTGTTTCATGGCCGGTGGAc                                                                                          <  2:7647575/140‑1 (MQ=255)

                                              cgcgGTCTGGCTGCCATTACTCAAAATCCGTTGCAAAAGTAATGAGTCATTTTTCTTGATTACAGGTGTTACCAACTCCCCAACTAACTCGGCGTCCTCACGCACTCGCCATAACCGGCCTTGTTTCATGGCCGGTGGAc                                                                                          >  1:8905111/1‑140 (MQ=255)

                                                  gTCTGGCTGCCATTACTCAAAATCCGTTGCAAAAGTAATGAGTCATTTTTCTTGATTACAGGTGTTACCAACTCCCCAACTAACTCGGCGTCCTCACGCACTCGCCATAACCGGCCTTGTTTCATGGCCGGTGGACaaaa                                                                                      >  2:6867928/1‑140 (MQ=255)

                                                  gTCTGGCTGCCATTACTCAAAATCCGTTGCAAAAGTAATGAGTCATTTTTCTTGATTACAGGTGTTACCAACTCCCCAACTAACTCGGCGTCCTCACGCACTCGCCATAACCGGCCTTGTTTCATGGCCGGTGGACaaaa                                                                                      >  1:4301785/1‑140 (MQ=255)

                                                   tCTGGCTGCCATTACTCAAAATCCGTTGCAAAAGTAATGAGTCATTTTTCTTGATTACAGGTGTTACCAACTCCCCAACTAACTCGGCGTCCTCACGCACTCGCCATAACCGGCCTTGTTTCATGGCCGGTGGACAaaat                                                                                     >  2:2037874/1‑140 (MQ=255)

                                                      ggCTGCCATTACTCAAAATCCGTTGCAAAATTAATGAGTCAATTTTCTAGATTACAGGTGTTACCAACTCCCCAACTACCACGGCTTCCTCACGCACTCGCCATAACCGGCCTTGTTTAATGGCCGGTGGACAaaataca                                                                                  <  2:327051/140‑3 (MQ=255)

                                                       gCTGCCATTACTCAAAATCCGTTGCAAAAGTAATGAGTCATTTTTCTTGATTACAGGTGTTACCAACTCCCCAACTAACTCGGCGTCCTCACGCACTCGCCATAACCGGCCTTGTTTCATGGCCGGTGGACAaaataaat                                                                                 >  2:4620234/1‑140 (MQ=255)

                                                        cTGCCATTACTCAAAATCCGTTGCAACAGTAATGAGTCATTTTTCTTGATTACAGGTGTTACCAACTCCCCAACTAACTCGGCGTCCTCACGCACTCGCCATAACCGGCCTTGTTTCATGGCCGGTGGACAAAATAAAtt                                                                                <  2:2873843/140‑1 (MQ=255)

                                                        cTGCCATTACTCAAAATCCGTTGCAAAAGTAATGAGTCATTTTTCTTGATTACAGGTGTTACCAACTCCCCAACTAACTCGGCGTCCTCACGCACTCGCCATAACCGGCCTTGTTTCATGGCCGGTGGACAAAATAAAtt                                                                                <  2:7050279/140‑1 (MQ=255)

                                                             actACTCAAAATCCGTTGCAACAGTACTGAGTCATTGTTCTTGATTACCGGTGTTACCAACTCCCCAACTAACGCGGCGTCCTCACGCACTCGCCTTAACCGGCCTTGTTTCATGGCCGGTGGACAAAATAAATTCTGCt                                                                           <  1:7320310/138‑1 (MQ=255)

                                                             aTTACTCAAAATCCGTTGCAAAAGTAATGAGTCATTTTTCTTGATTACAGGTGTTACCAACTCCCCAACTAACTCGGCGTCCTCACGCACTCGCCATAACCGGCCTTGTTTCATGGCCGGTGGACAAAATAAATTCTGCt                                                                           >  2:9497250/1‑140 (MQ=255)

                                                             aTTACTCAAAATCCGTTGCAAAAGTAATGAGTCATTTTTCTTGATTACAGGTGTTACCAACTCCCCAACTAACTCGGCGTCCTCACGCACTCGCCATAACCGGCCTTGTTTCATGGCCGGTGGACAAAATAAATTCTGCt                                                                           >  1:2826510/1‑140 (MQ=255)

                                                             aTTACTCAAAATCCGTTGCAAAAGTAATGAGTCATTTTTCTTGATTACAGGTGTTACCAACTCCCCAACTAACTCGGCGTCCTCACGCACTCGCCATAACCGGCCTTGTTTCATGGCCGGTGGACAAAATAAATTCTGCt                                                                           >  1:3260833/1‑140 (MQ=255)

                                                             aTTACTCAAAATCCGTTGCAAAAGTAATGAGTCATTTTTCTTGATTACAGGTGTTACCAACTCCCCAACTAACTCGGCGTCCTCACGCACTCGCCATAACCGGCCTTGTTTCATGGCCGGTGGACAAAATAAATTCTGCt                                                                           >  1:591207/1‑140 (MQ=255)

                                                                        tCCGTTGCAAAAGTAATGAGTCATTTTTCTTGATTACAGGTGTTACCAACTCCCCAACTAACTCGGCGTCCTCACGCACTCGCCATAACCGGCCTTGTTTCATGGCCGGTGGACAAAATAAATTCTGCTTAGCATAacga                                                                >  2:2535932/1‑140 (MQ=255)

                                                                         ccGTTGCAAAAGTAATGAGTCATTTTTCTTGATTACAGGTGTTACCAACTCCCCAACTAACTCGGCGTCCTCACGCACTCGCCATAACCGGCCTTGTTTCATGGCCGGTGGACAAAATAAATTCTGCTTAGCATAacgac                                                               <  1:1008279/140‑1 (MQ=255)

                                                                         ccGTTGCAAAAGTAATGAGTCATTTTTCTTGATTACAGGTGTTACCAACTCCCCAACTAACTCGGCGTCCTCACGCACTCGCCATAACCGGCCTTGTTTCATGGCCGGTGGACAAAATAAATTCTGCTTAGCATAacgac                                                               <  1:4555828/140‑1 (MQ=255)

                                                                         ccGTTGCAAAAGTAATGAGTCATTTTTCTTGATTACAGGTGTTACCAACTCCCCAACTAACTCGGCGTCCTCACGCACTCGCCATAACCGGCCTTGTTTCATGGCCGGTGGACAAAATAAATTCTGCTTAGCATAacgac                                                               <  1:9304646/140‑1 (MQ=255)

                                                                          cGTTGCAAAAGTAATGAGTCATTTTTCTTGATTACAGGTGTTACCAACTCCCCAACTAACTCGGCGTCCTCACGCACTCGCCATAACCGGCCTTGTTTCATGGCCGGTGGACAAAATAAATTCTGCTTAGCATAacgacg                                                              <  1:471293/140‑1 (MQ=255)

                                                                          cGTTGCAAAAGTAATGAGTCATTTTTCTTGATTACAGGTGTTACCAACTCCCCAACTAACTCGGCGTCCTCACGCACTCGCCATAACCGGCCTTGTTTCATGGCCGGTGGACAAAATAAATTCTGCTTAGCATAacgacg                                                              <  2:2658542/140‑1 (MQ=255)

                                                                           gTTGCAAAAGTAATGAGTCATTTTTCTTGATTACAGGTGTTACCAACTCCCCAACTAACTCGGCGTCCTCACGCACTCGCCATAACCGGCCTTGTTTCATGGCCGGTGGACAAAATAAATTCTGCTTAGCATAACGACGc                                                             <  1:4169568/140‑1 (MQ=255)

                                                                            ttGCAAAAGTAATGAGTCATTTTTCTTGATTACAGGTGGTACCAACTCCCCAACTAACTCGGCGTCCTCACGCACTCGCCATAACCGGCCTTGTTTCATGGCCGGTGGACAAAATAAATTCTGCTTAGCATAACGACGCa                                                            >  1:5972672/1‑140 (MQ=255)

                                                                             tGCAAAAGTAATGAGTCATTTTTCTTGATTACAGGTGTTACCAACTCCCCAACTAACTCGGCGTCCTCACGCACTCGCCATAACCGGCCTTGTTTCATGGCCGGTGGACAAAATAAATTCTGCTTAGCATAACGACGCaa                                                           <  1:2209401/140‑1 (MQ=255)

                                                                             tGCAAAAGTAATGAGTCATTTTTCTTGATTACAGGTGTTACCAACTCCCCAACTAACTCGGCGTCCTCACGCACTCGCCATAACCGGCCTTGTTTCATGGCCGGTGGACAAAATAAATTCTGCTTAGCATAACGACGCaa                                                           <  2:1283924/140‑1 (MQ=255)

                                                                              gCAAAAGTAATGAGTCATTTTTCTTGATTACAGGTGTTACCAACTCCCCAACTAACTCGGCGTCCTCACGCACTCGCCATAACCGGCCTTGTTTCATGGCCGGTGGACAAAATAAATTCTGCTTAGCATAACGACGCAAt                                                          <  1:9165538/140‑1 (MQ=255)

                                                                              gCAAAAGTAATGAGTCATTTTTCTTGATTACAGGTGTTACCAACTCCCCAACTAACTCGGCGTCCTCACGCACTCGCCATAACCGGCCTTGTTTCATGGCCGGTGGACAAAATAAATTCTGCTTAGCATAACGACGCAAt                                                          <  2:4849264/140‑1 (MQ=255)

                                                                                   aGTAATGAGTCATTTTTCTTGATTACAGGTGTTACCAACTCCCCAACTAACTCGGCGTCCTCACGCACTCGCCATAACCGGCCTTGTTTCATGGCCGGTGGACAAAATAAATTCTGCTTAGCATAACGACGCAATGTGGa                                                     <  1:235301/140‑1 (MQ=255)

                                                                                   aGTAATGAGTCATTTTTCTTGATTACAGGTGTTACCAACTCCCCAACTAACTCGGCGTCCTCACGCACTCGCCATAACCGGCCTTGTTTCATGGCCGGTGGACAAAATAAATTCTGCTTAGCATAACGACGCAATGTGGa                                                     <  2:8933029/140‑1 (MQ=255)

                                                                                   aGTAATGAGTCATTTTTCTTGATTACAGGTGTTACCAACTCCCCAACTAACTCGGCGTCCTCACGCACTCGCCATAACCGGCCTTGTTTCATGGCCGGGGGACAAAATAAATTCTGCTGAGCATAACGACGCAATGTGGa                                                     <  2:4702104/140‑1 (MQ=255)

                                                                                   aGTAATGAGTCATTTTTCTTGATTACAGGTGTTACCAACTCCCCAACTAACTCGGCGTCCTCACGCACTCGCCATAACCGGCCTCGTTTCATGGCCGGTGGACAAAATAAATTCTGCTTAGCATAACGACGCAATGTGGa                                                     <  1:685574/140‑1 (MQ=255)

                                                                                   aGTAATGAGTCATTTTACTTGATTACAGGTGTTACCAACTCCCCAACGAACTCGGCGTCCTCACGCACTCGCCATAACCGGCCGTGTTTCATGGCCGGTGGACAAAATAAATTCTGCTTAGCATAACGACGCAATGTGGa                                                     <  2:8630454/140‑1 (MQ=255)

                                                                                    gTAATGAGTCATTTTTCTTGATTACAGGTGTTACCAACTCCCCAACTAACTCGGCGTCCTCACGCACTCGCCATAACCGGCCTTGTTTCATGGCCGGTGGACAACATAAATTCTGCTTAGCATAACGACGCAATGTGGac                                                    <  2:7323645/140‑1 (MQ=255)

                                                                                     tAATGAGTCATTTTTCTTGATTACAGGTGTTACCAACTCCCCAACTAACTCGGCGTCCTCACGCACTCGCCATAACCGGCCTTGTTTCATGGCCGGTGGACAAAATAAATTCTGCTTAGCATAACGACGCAATGTGGaca                                                   <  2:245197/140‑1 (MQ=255)

                                                                                         gAGTCATTTTTCTTGATTACAGGTGTTACCAACTCCCCAACTAACTCGGCGTCCTCACGCACTCGCCATAACCGGCCTTGTTTCATGGCCGGTGGACAAAATAAATTCTGCTTAGCATAACGACGCAATGTGGACACACt                                               <  2:439719/140‑1 (MQ=255)

                                                                                         gAGTCATTTTTCTTGATTACAGGTGTTACCAACTCCCCAACTAACTCGGCGTCCTCACGCACTCGCCATAACCGGCCTTGTTTCATGGCCGGTGGACAAAATAAATTCTGCTTAGCATAACGACGCAATGTGGACACACt                                               <  2:5206150/140‑1 (MQ=255)

                                                                                          aGTCATTTTTCTTGATTACAGGTGTTACCAACTCCCCAACTAACTCGGCGTCCTCACGCACTCGCCATAACCGGCCTTGTTTCATGGCCGGTGGACAAAATAAATTCTGCTTAGCATAACGACGCAATGTGGACACACtt                                              >  1:8472265/1‑140 (MQ=255)

                                                                                          aGTCATTGTTCTTGATTACAGGTGTTACCAACTCCCCCACTAACTCGGCGTCCTCACGCACTCGCCATAACCGGCCTTGTTTCATGGCCGGTGGACAAAATCAATTCTGCTTAGCATAACGACGCACTGTGGACACACtt                                              >  1:7041661/1‑140 (MQ=255)

                                                                                            tCATTTTTCTTGATTACAGGTGTTACCAACTCCCCAACTAACTCGGCGTCCTCACGCACTCGCCATAACCGGCCTTGTTTCATGGCCGGTGGACAAAATAAATTCTGCTTAGCATAACGACGCAATGTGGACACACTTgg                                            <  1:5050872/140‑1 (MQ=255)

                                                                                            tCATTTTTCTTGATTACAGGTGTTACCAACTCCCCAACTAACTCGGCGTCCTCACGCACTCGCCATAACCGGCCTTGTTTCATGGCCGGTGGACAAAATAAATTCTGCTTAGCATAACGACGCAATGTGGACACACTTgg                                            <  1:3106514/140‑1 (MQ=255)

                                                                                                 tttCTTGATTACAGGTGTTACCAACTCCCCCACTAACTCGGCGTCCTCACGCACTCGCCATAACCGGCCTTGTTTCATGGCCGGTGGACAAAATAAATTCTGCTTAGCATAACGACGCAATGTGGACACACTTGGAGGAt                                       <  2:8444446/140‑1 (MQ=255)

                                                                                                 tttCTTGATTACAGGTGTTACCAACTCCCCAACTAACTCGGCGTCCTCACGCACTCGCCATAACCGGCCTTGTTTCATGGCCGGTGGACAAAATAAATTCTGCTTAGCATAACGACGCAATGTGGACACACTTGGAGGAt                                       <  2:3412568/140‑1 (MQ=255)

                                                                                                 tttCTTGAGTACAGGTGTTACCAACTCCCCAACTAACTCGGCGTCCTCACGCACTCGCCATAAACGGCCTTGTTTCATGGCCGTTTGACAAAATAAATTCTGCTTATCATAACTACGAAATGTGGACACACTTGGAGGAt                                       <  2:9314295/140‑1 (MQ=255)

                                                                                                 tgtcatGATTACAGGTGTTACCAACGCCCCAACGAACTCGGCGTCCTCACGCACTCGCCATAACCGGCCTTGTGTCATGGCCGGTGGACTCAATACATTCTGCGTAGCATAACGACGCAATGTGGACACACTTggaggag                                       <  2:7195373/135‑2 (MQ=255)

                                                                                                                  ttACCAACTCCCCAACTAACTCGGCGTCCTCACGCACTCGCCATAACCGGCCTTGTTTCATGGCCGGTGGACAAAATAAATTCTGCTTAGCATAACGACGCAATGTGGACACACTTGGAGGATTACTTCTGTATTTTTca                      >  1:8293710/1‑140 (MQ=255)

                                                                                                                   tACCAACTCCCCAACTAACTCGGCGTCCTCACGCACTCGCCATAACCGGCCTTGTTTCATGGCCGGTGGACAAAATAAATTCTGCTTAGCATAACGACGCAATGTGGACACACTTGGAGGATTACTTCTGTATTTTTcag                     <  1:122671/140‑1 (MQ=255)

                                                                                                                    aCCAACTCCCCAACTAACTCGGCGTCCTCACGCACTCGCCATAACCGGCCTTGTTTCATGGCCGGTGGACAATATAAATTCTGCTTAGCATAACGACGCAATGTGGACACACTGGGAGGATTACTTCTGTATTTTTcagc                    >  1:7455606/1‑140 (MQ=255)

                                                                                                                        aCTCCCCACCTCACTCGGCGTCCTCACGCACTCGCCATAACCGGCCTTGGTTCATGGCCGGTGGACAAAATAAATTCTGCTTAGCATAACGACGCAATGTGGACACACTTGGAGGATTACTTCTGTATTTTTCAGCAGcc                <  2:3604648/140‑1 (MQ=255)

                                                                                                                          tCCCCAACTAACTCGGCGTCCTCACGCACTCGCCATAACCGGCCTTGTTTCATGGCCGGTGGACAAAATAAATTCTGCTTAGCATAACGACGCAATGTGGACACACTTGGAGGATTACTTCTGTATTTTTCAGCAGCCCa              >  1:360528/1‑140 (MQ=255)

                                                                                                                          tCCCCAACTAACTCGGCGTCCTCACGCACTCGCCATAACCGGCCTTGTTTCATGGCCGGTGGACAAAATAAATTCTGCTTAGCATAACGACGCAATGTGGACACACTTGGAGGATTACTTCTGTATTTTTCAGCAGCCCa              >  2:5155210/1‑140 (MQ=255)

                                                                                                                          tCCCCAACTAACTCGGCGTCCTCACGCACTCGCCATAACCGGCCTTGTTTCATGGCCGGTGGACAAAATAAATTCTGCTTAGCATAACGACGCAATGTGGACACACTTGGAGGATTACTTCTGTATTTTTCAGCAGCCCa              <  1:9012044/140‑1 (MQ=255)

                                                                                                                              cAACTAACTCGGCGTCCTCACGCACTCGCCATAACCGGCCTTGTTTCATGGCCGGTGGACAAAATAAATTCTGCTTAGCATAACGACGCAATGTGGACACACTTGGAGGATTACTTCTGTATTTTTCAGCAGCCCAttct          >  1:6223947/1‑140 (MQ=255)

                                                                                                                               aactaactCGGCGTCCTCACGCACTCGCCATAACCGGCCTTGTTTCATGGCCGGTGGACAAAATAAATTCTGCTTAGCATAACGACGCAATGTGGACACACTTGGAGGATTACTTCTGTATTTTTCAGCAGCCCAttctt         <  2:8186697/140‑1 (MQ=255)

                                                                                                                                 ctaactCGGCGTCCTCACGCACTCGCCATAACCGGCCTTGTTTCATGGCCGGTGGACAAAATAAATTCTGCTTAGCATAACGACGCAATGTGGACACACTTGGAGGATTACTTCTGTATTTTTCAGCAGCCCATTCTTCa       >  2:3852415/1‑140 (MQ=255)

                                                                                                                                 ctaactCGGCGTCCTCACGCACTCGCCATAACCGGCCTTGTTTCATGGCCGGTGGACAAAATAAATTCTGCTTAGCATAACGACGCAATGTGGACACACTTGGAGGATTACTTCTGTATTTTTCAGCAGCCCATTCTTCa       >  1:872508/1‑140 (MQ=255)

                                                                                                                                    actCGGCGTCCTCACGCACTCGCCATAACCGGCCTTGTTTCATGGCCGGTGGACAAAATAAATTCTGCTTAGCATAACGACGCAATGTGGACACACTTGGAGGATTACTTCTGTATTTTTCAGCAGCCCATTCTTCAaga    >  1:4014192/1‑140 (MQ=255)

                                                                                                                                    actCGGCGTCCTCACGCACTCGCCATAACCGGCCTTGTTTCATGGCCGGTGGACAAAATAAATTCTGCTTAGCATAACGACGCAATGTGGACACACTTGGAGGATTACTTCTGTATTTTTCAGCAGCCCATTCTTCAaga    >  1:1953620/1‑140 (MQ=255)

CCTACTATAAAGCGGATATAAGTTAGGGACTGAAACATTATTCTTACGCGGTCTGGCTGCCATTACTCAAAATCCGTTGCAAAAGTAATGAGTCATTTTTCTTGATTACAGGTGTTACCAACTCCCCAACTAACTCGACGTCCTCACGCACTCGCCATAACCGGCCTTGTTTCATGGCCGGTGGACAAAATAAATTCTGCTTAGCATAACGACGCAATGTGGACACACTTGGAGGATTACTTCTGTATTTTTCAGCAGCCCATTCTTCAAGAGT  >  NZ_CP025268/1207845‑1208118

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`