BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP025268	1,236,662	T→C	A277A (GCA→GCG)	hlyE ←	hemolysin HlyE

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP025268	1,236,662	0	T	C	99.7%	2083.5 / NA	597	A277A (GCA→GCG)	hlyE	hemolysin HlyE
Reads supporting (aligned to +/- strand): ref base T (0/0); major base C (403/192); minor base A (1/1); total (404/193)
Fisher's exact test for biased strand distribution p-value = 5.42e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 599 total aligned reads displayed.

GTTTTTAAATGTGATAAACCTTATACCTTGAGTACATGGAGAGAATAATCGCTTATCAGACTTCAGGTACCTCAAAGAGTGTCTTTTTACCGTGTCTTTTCTGATACTCATTACAGGTGTTAATCATTTTTTTGGCTGCTTCTTTTAGCAAAGAAAGCATTAAATCATCATAATCAACGTAGAATCTGGTTGTTTCAGTTTCCGTTTTTATCTCACCGATGGCGGCTATTTCGGTGGTTAATTTCAATTTGGCGGCATCGATATCTTTATTCG  >  NZ_CP025268/1236526‑1236798

gtTTTTAAATGTGATAAACCTTATACCTTGAGTACATGGAGAGAATAATCGCTTATCAGACTTCAGGTACCTCAAAGAGTGTCTTTTTACCGTGTCTTTTCTGATACTCATTACAGGTGTTAATCATTTTTTTGgccgct                                                                                                                                       <  1:1013759/140‑1 (MQ=255)

    ttAAATGTGATAAACCTTATACCTTGATTACATGGAGAGAATAATCGCTTATCAGACTTCAGGTACCTCAAAGAGTGTCTTTTTACCGTGTCTTTTCTGATACTCATTACAGGTGTTAATCATTTTTTTGGCCGcttctt                                                                                                                                   <  1:1634305/140‑1 (MQ=255)

         tgtgATAAACCTTATACCTTGAGTACATGGAGAGAATACTCGCTTATCAGACTTCAGGGACCTCAAAGAGTGTCTTTTTACCGTGTCTTTTCAGATACTCATTACAGGTGTTAATCATTTTTTTGGCCGCTTCTTTTAGc                                                                                                                              <  2:8440399/140‑1 (MQ=255)

           tgATAAACCTTATACCTTGAGTACATGGAGAGAATAATCGCTTATCAGACTTCAGGTACCTCAAAGAGTGTCTTTTTACCGTGTCTTTTCTGATACTCATTACAGGTGTTAATCATTTTTTTGGCCGCTTCTTTTAGCaa                                                                                                                            >  1:8250036/1‑140 (MQ=255)

              tAAACCTTATACCTTGAGTACATGGAGAGAATAATCGCTTATCAGACTTCAGGTACCTCAAAGAGTGTCTTTTTACCGTGTCTTTTCTGATACTCATTACAGGTGTTAATCATTTTTTTGGCCGCTTCTTTTAGCaaaga                                                                                                                         <  2:7955639/140‑1 (MQ=255)

              tAAACCTTATACCTTGAGTACATGGAGAGAATAATCGCTTATCAGACTTCAGGTACCTCAAAGAGTGTCTTTTTACCGTGTCTTTTCTGATACTCATTACAGGTGTTAATCATTTTTTTGGCCGCTTCTTTTAGCaaaga                                                                                                                         <  1:5674985/140‑1 (MQ=255)

              tAAACCTTATACCTTGAGTACATGGAGAGAATAATCGCTTATCAGACTTCAGGTACCTCAAAGAGTGTCTTTTTACCGTGTCTTTTCTGATACTCATTACAGGTGTTAATCATTTTTTTGGCCGCTTCTTTTAGCaaaga                                                                                                                         >  1:4129481/1‑140 (MQ=255)

               aaaCCTTATACCTTGAGTACATGGAGAGAATAATCGCTTATCAGACTTCAGGTACCTCAAAGAGTGTCTTTTTACCGTGTCTTTTCTGATACTCATTACAGGTGTTAATCATTTTTTTGGCCGCTTCTTTTAGCaaagaa                                                                                                                        <  2:541650/140‑1 (MQ=255)

                     tataCCTTGAGTACATGGAGAGAATAATCGCTTATCAGACTTCAGGTACCTCAAAGAGTGTCTTTTTACCGTGTCTTTTCTGATACTCATTACAGGTGTTAATCATTTTTTTGGCCGCTTCTTTTAGCAAAGAAAGCAtt                                                                                                                  >  1:328823/1‑140 (MQ=255)

                        aCCTTGAGTACATGGAGAGAATAATCGCTTATCAGACTTCAGGTACCTCAAAGAGTGTCTTTTTACCGTGTCTTTTCTGATACTCATTACAGGTGTTAATCATTTTTTTGGCCGCTTCTTTTAGCAAAGAAAGCATTaaa                                                                                                               <  1:7965579/140‑1 (MQ=255)

                        aCCTAGAGTACATGGAGAGAAAAATCGATTATCAGACTTCAGGAACCTCAAAGAGTGTCTTATTACCGTGTCTTTTCTGATACTCATTACAGGTGTTAATCATTTTTTTGGCCGCTTCTTTTAGCAAAGAAAGCATTaaa                                                                                                               <  2:746846/140‑1 (MQ=255)

                          cTTGAGTACATGGAGAGAATAATCGCTTATCAGACTTCAGGTACCTCAAAGAGTGTCTTTTTACCGTGTCTTTTCTGATACTCATTACAGGTGTTAATCATTTTTTTGGCCGCTTCTTTTAGCAAAGAAAGCATTAAatc                                                                                                             >  2:2278603/1‑140 (MQ=255)

                            tgtgTACATGGAGAGTATAATCGATTATCAGACTTCAGGTACCTCAAAGAGTGTCTTTTTACCTTTTATTTTCTGAGAGTCATTACAGGTGTTAATCATTTTTTTGGCCGCTTCTTTTAGCAATGAAAGCTTTAAatcag                                                                                                           >  2:5246628/4‑139 (MQ=255)

                            tGAGTACATGGAGAGAATAATCGCTTATCAGACTTCAGGTCCCTCAAAGAGTGTCTTTTTACCGTGTCTTTTCTGATACTCATTACAGGTGTTAATCATTTTTTTGGCCGCTTCTGTTAGCAAAGAAAGCAGTAAatcat                                                                                                           >  1:2411072/1‑140 (MQ=255)

                            tGAGTACATGGAGAGAATAATCGCTTATCAGACTTCAGGTACCTCAAAGAGTGTCTTTTTACCGTGTCTTTTCTGATACTCATTACAGGTGTTAATCATTTTTTTGGCCGCTTCTTTTAGCAAAGAAAGCATTAAatcat                                                                                                           >  2:7343964/1‑140 (MQ=255)

                            tGAGTACATGGAGAGAATAATCGCTTATCAGACTTCAGGTACCTCAAAGAGTGTCTTTTTACCGTGTCTTTTCTGATACTCATTACAGGTGTTAATCATTTTTTTGGCCGCTTCTTTTAGCAAAGAAAGCATTAAatcat                                                                                                           >  2:1239103/1‑140 (MQ=255)

                            tGAGTACATGGAGAGAATAATCGCTTATCAGACTTCAGGTACCTCAAAGAGTGTCTTTTTACCGTGTCTTTTCTGATACTCATTACAGGTGTTAATCATTTTTTTGGCCGCTTCTTTTAGCAAAGAAAGCATTAAatcat                                                                                                           >  1:6517775/1‑140 (MQ=255)

                            tGAGTACATGGAGAGAATAATCGCTTATCAGACTTCAGGTACCTCAAAGAGTGTCTTTTTACCGTGTCTTTTCTGATACTCATTACAGGTGTTAATCATTTTTTTGGCCGCTTCTTTTAGCAAAGAAAGCATTAAatcat                                                                                                           >  1:502928/1‑140 (MQ=255)

                            tGAGTACATGGAGAGAATAATCGCTTATCAGACTTCAGGTACCTCAAAGAGTGTCTTTTTACCGTGTCTTTTCTGATACTCATTACAGGTGTTAATCATTTTTTTGGCCGCTTCTTTTAGCAAAGAAAGCATTAAatcat                                                                                                           >  1:2578171/1‑140 (MQ=255)

                            tGAGTACATGGAGAGAATAATCGCTTATCAGACTTCAGGTACCTCAAAGAGTGTCTTTTTACCGTGTCTTTTCTGAAACTCATTACAGGTGTTAATCATTTTTTTGGCCGATTCTTTTAGCAAAGAAAGCATTAAatcaa                                                                                                           >  2:3383676/1‑139 (MQ=255)

                             gAGTACATGGAGAGAATAATCGCTTATCAGACTTCAGGTACCTCAAAGAGTGTCTTTTTACCGTGTCTTTTCTGATACTCATTACAGGTGTTAATCATTTTTTTGGCCGCTTCTTTTAGCAAAGAAAGCATTAAatcatc                                                                                                          >  1:9552174/1‑140 (MQ=255)

                             gAGTACATGGAGAGAATAATCGCTTATCAGACTTCAGGTACCTCAAAGAGTGTCTTTTTACCGTGTCTTTTCTGATACTCATTACAGGTGTTAATCATTTTTTTGGCCGCTTCTTTTAGCAAAGAAAGCATTAAatcatc                                                                                                          >  1:4461272/1‑140 (MQ=255)

                              aGTACATGGAGAGAATAATCGCTTATCAGACTTCAGGTACCTCAAAGAGTGTCTTTTTACCGTGTCTTTTCTGATACTCATTACAGGTGTTAATCATTTTTTTGGCCGCTTCTTTTAGCAAAGAAATCATTAAAtcatca                                                                                                         >  1:2067612/1‑140 (MQ=255)

                                 aCATGGAGAGAATAATCGCTTATCAGACTTCAGGTACCTCAAAGAGTGTCTTTTTACCGTGTCTTTTCTGATACTCATTACAGGTGTTAATCATTTTTTTGGCCGCTTCTTTTAGCAAAGAAAGCATTAATTCATCATaa                                                                                                      >  2:5558518/1‑140 (MQ=255)

                                 aCATGGAGAGAATAATCGCTTATCAGACTTCAGGTACCTCAAAGAGTGTCTTTTTACCGTGACTTTTCTGATACTCATTACAGGTGTTAATCATTTTTTTGGCCGCTTCTTTTATCAAAGAAAGCATTAAATAATCATat                                                                                                      >  2:4378235/1‑139 (MQ=255)

                                 aCATGGAGAGAATAAACGCTTATCAGACTTCAGGTACCTCAAAGAGTGTCTTTTTACCGTGTCTTTTCTGATCCTCATTACAGGTGTTAATCATTTTTTTTGCCGCTTCTTTTAGCAAAGAAAGCATTAAATCATCATaa                                                                                                      >  2:3468349/1‑140 (MQ=255)

                                  cATGGAGAGAATAATCGCTTATCAGACTTCAGGTACCTCAAAGAGTGTCTTTTTACCGTGTCTTTTCTGATACTCATTACAGGTGTTAATCATTTTTTTGGCCGCTTCTTTTAGCAAAGAAAGCATTAAATCATCATAAt                                                                                                     <  1:8431144/140‑1 (MQ=255)

                                  cATGGAGAGAATAATCGCTTATCAGACTTCAGGTACCTCAAAGAGTGTCTTTTTACCGTGTCTTTTCTGATACTCATTACAGGTGTTAATCATTTTTTTGGCCGCTTCTTTTAGCAAAGAAAGCATTAAATCATCATAAt                                                                                                     <  2:7845716/140‑1 (MQ=255)

                                   aTGGAGAGAATAATCGCTTATCAGACTTCAGGTACCTCAAAGAGTGTCTTTTTACCGTGTCTTTTCTGATACTCATTACAGGTGTTAATCATTTTTTTGGCCGCTTCTTTTAGCAAAGAAAGCATTAAATCATCATAATc                                                                                                    >  1:6226416/1‑140 (MQ=255)

                                   aTGGAGAGAATAATCGCTTATCAGACTTCAGGTACCTCAAAGAGTGTCTTTTTACCGTGTCTTTTCTGATACTCATTACAGGTGTTAATCATTTTTTTGGCCGCTTCTTTTAGCAAAGAAAGCATTAAATCATCATAATc                                                                                                    >  2:5719498/1‑140 (MQ=255)

                                   aTGGAGAGAATAATCGCTTATCAGACTTCAGGTACCTCAAAGAGTGTCTTTTTACCGTGTCTTTTCTGATACTCATTACAGGTGTTAATCATTTTTTTGGCCGCTTCTTTTAGCAAAGAAAGCATTAAATCATCATAATc                                                                                                    <  1:9047680/140‑1 (MQ=255)

                                   aTGGAGAGAATAATCGCTTATCAGACTTCAGGTACCTCAAAGAGTGTCTTTTTACCGTGTCTTTTCTGATACTAATTACAGGTGTTAATCATTTTTTTGGCCGCTTCTTTTAGCAAAGAAAGCATTAAATCATCATAATc                                                                                                    <  1:4595877/140‑1 (MQ=255)

                                    tGGAGAGAATAATCGCTTATCAGACTTCAGGTACCTCAAAGAGTGTCTTTTTACCGTGTCTTTTCTGATACTCATTACAGGTGTTAATCATTTTTTTGGCCGCTTCTTTTAGCAAAGAAAGCATTAAATCATCATAATCa                                                                                                   >  1:1951164/1‑140 (MQ=255)

                                     ggAGAGAATAATCGCTTATCAGACTTCAGGTACCTCAAAGAGTGTCTTTTTACCGTGTCTTTTCTGATACTCATTACAGGTGTTAATCATTTTTTTGGCCGCTTCTTTTAGCAAAGAAAGCATTAAATCATCATAATCaa                                                                                                  >  2:6202610/1‑140 (MQ=255)

                                        gagaATAATCGCTTATCAGACTTCAGGTACCTCAAAGAGTGTCTTTTTACCGTGTCTTTTCTGATACTCATTTCAGGTGTTAGTCATTTTTTTGGCCGCTTCTTTTAGCCAAGACAGCATTAAATCTTCATAATCTACGt                                                                                               >  1:9321128/1‑140 (MQ=255)

                                        gagaATAATCGCTTATCAGACTTCAGGTACCTCAAAGAGTGTCTTTTTACCGTGTCTTTTCTGATACTCATTACAGGTGTTAATCATTTTTTTGGCCGCTTCTTTTAGCAAAGAAAGCATTAAATCATCATAATCAACGt                                                                                               >  2:2163430/1‑140 (MQ=255)

                                        gagaATAATCGCTTATCAGACTTCAGGTACCTCAAAGAGTGTCTTTTTACCGTGTCTTTTCTGATACTCATTACAGGTGTTAATCATTTTTTTGGCCGCTTCTTTTAGCAAAGAAAGCATTAAATCATCATAATCAACGt                                                                                               >  1:7765790/1‑140 (MQ=255)

                                          gaATAATCGCTTATCAGACTTCAGGTACCTCAAAGAGTGTCTTTTTACCGTGTCTTTTCTGATACTCATTACAGGTGTTAATCATTTTTTTGGCCGCTTCTTTTAGCAAAGAAAGCATTAAATCATCATAATCAACGTAg                                                                                             >  1:6338240/1‑140 (MQ=255)

                                          gaATAATCGCTTATCAGACTTCAGGTACCTCAAAGAGTGTCTTTTTACCGTGTCTTTTCTGATACTCATTACAGGTGTTAATCATTTTTTTGGCCGCTTCTTTTAGCAAAGAAAGCATTAAATCATCATAATCAACGTAg                                                                                             >  2:871285/1‑140 (MQ=255)

                                             taatCGCTTATCAGACTTCAGGTACCTCAAAGAGTGTCTTTTTACCGTGTCTTTTCTGATACTCATTACAGGTGTTAATCATTTTTTTGGCCGCTTCTTTTAGCAAAGAAAGCATTAAATCATCATAATCAACGTAGAAt                                                                                          <  2:6048897/140‑1 (MQ=255)

                                                tCGCTTATCAGACTTCAGGTACCTCAAAGAGTGTCTTTTTACCGTGTCTTTTCTGATACTCATTACAGGTGTTAATCATTTTTTTGGCCGCTTCTTTTAGCAAAGAAAGCATTAAATCATCATAATCAACGTAGAATCTg                                                                                       >  2:3032481/1‑140 (MQ=255)

                                                tCGCTTATCAGACTTCAGGTACCTCAAAGAGTGTCTTTTTACCGTGTCTTTTCTGATACTCATTACAGGTGTTAATCATTTTTTTGGCCGCTTCTTTTAGCAAAGAAAGCATTAAATCATCATAATCAACGTAGAATCTg                                                                                       >  1:7303499/1‑140 (MQ=255)

                                                tCGCTTATCAGACTTCAGGTACCTCAAAGAGTGTCTTTTTACCGTGTCTTTTCTGATACTCATTACAGGTGTTAATCATTTTTTTGGCCGCTTCTTTTAGCAAAGAAAGCATTAAATCATCATAATCAACGTAGAATCTg                                                                                       >  1:3769417/1‑140 (MQ=255)

                                                 cGCTTATCAGACTTCAGGTACCTCAAAGAGTGTCTTTTTACCGTGTCTTTTCTGATACTCATTACAGGTGTTAATCATTTTTTTGGCCGCTTCTTTTAGCAAAGAAAGCATTAAATCATCATAATCAACGTAGAATCTgg                                                                                      <  1:5273813/140‑1 (MQ=255)

                                                   cTTATCAGACTTCAGGTACCTCAAAGAGTGTCTTTTTACCGTGTCTTTTCTGATACTCATTACAGGTGTTAATCATTTTTTTGGCCGCTTCTTTTAGCAAAGAAAGCATTAAATCATCATAAt                                                                                                     >  2:1288362/1‑123 (MQ=255)

                                                   cTTATCAGACTTCAGGTACCTCAAAGAGTGTCTTTTTACCGTGTCTTTTCTGATACTCATTACAGGTGTTAATCATTTTTTTGGCCGCTTCTTTTAGCAAAGAAAGCATTAAATCATCATAAt                                                                                                     <  1:1288362/123‑1 (MQ=255)

                                                             ttCAGGTACCTCAAAGAGTGTCTTTTTACCGTGTCTTTTCTGATACTCATTACAGGTGTTAATCATTTTTTTGGCCGCTTCTTTTAGCAAAGAAAGCATTAAATCATCATAATCAACGTAGAATCTGGTTGTTTCAGttt                                                                          <  2:1596267/140‑1 (MQ=255)

                                                              tCAGGTACCTCAAAGAGTGTCTTTTTACCGTGTCTTTTCTGATACTCATTACAGGTGTTAATCATTTTTTTGGCCGCTTCTTTTAGCAAAGAAAGCATTAAATCATCATAATCAACGTAGAATCTGGTTGTGTCAGTTTc                                                                         >  1:9512169/1‑140 (MQ=255)

                                                                  gTACCTCAAAGAGTGTCTTTTTACCGTGTCTTTTCTGATACTCATTACAGGTGTTAATCATTTTTTTGGCCGCTTCTTTTAGCAAAGAAAGCATTaaa                                                                                                               >  2:8292575/1‑98 (MQ=255)

                                                                  gTACCTCAAAGAGTGTCTTTTTACCGTGTCTTTTCTGATACTCATTACAGGTGTTAATCATTTTTTTGGCCGCTTCTTTTAGCAAAGAAAGCATTaaa                                                                                                               <  1:8292575/98‑1 (MQ=255)

                                                                  gTACCTCAAAGAGTGTCTTTTTACCGTGTCTTTTCTGATACTCATTAAAGGTGTTAATCATTTTTTTGGCCGCTTCTTTTAGCAAAGAATGCAGTATATCATCATAATCAACGTAGTATCTGGTTGTTTCTGTGTCCGtg                                                                     >  1:7590435/1‑139 (MQ=255)

                                                                     ccTCAAAGAGTGTCTTTTTACCGTGTCTTTTCTGATACTCATTACAGGTGTTAATCATTTTTTTGGCCGCTTCTTTTAGCAAAGAAAGCATTAAATCATCATAATCAACGTAGAATCTGGTTGTTTCAGTTTCCGttttt                                                                  <  1:644689/140‑1 (MQ=255)

                                                                      cTCAAAGAGTGTCTTTTTACCGTGTCTTTTCTGATACTCATTACAGGTGTTAATCATTTTTTTGGCCGCTTCTTTTAGCAAAGAAAGCAt                                                                                                                   >  2:2803610/1‑90 (MQ=255)

                                                                      cTCAAAGAGTGTCTTTTTACCGTGTCTTTTCTGATACTCATTACAGGTGTTAATCATTTTTTTGGCCGCTTCTTTTAGCAAAGAAAGCAt                                                                                                                   <  1:2803610/90‑1 (MQ=255)

                                                                       tCAAAGAGTGTCTTTTTACCGTGTCTTTTCTGATACTCATTACAGGTGTTAATCATTTTTTTGGCCGCTTCTTTTAGCAAAGAAAGCATTAAATCATCATAATCAACGTAGAATCTGGTTGTTTCAGTTTCCGTTTTTAt                                                                >  1:3871468/1‑140 (MQ=255)

                                                                             agTGTCTTTTTACCGTGTCTTTTCTGATACTCATTACAGGTGTTAATCATTTTTTTGGCCGCTTCTTTTAGCAAAGAAAGCATTAAATCATCATAATCAACGTAGAATCTGGTTGTTTCAGTTTCCGTTTTTATCTCAcc                                                          >  1:4037485/1‑140 (MQ=255)

                                                                             agTGTCTTTTTACCGTGTCTTTTCTGATACTCATTACAGGTGTTAATCATTTTTTTGGCCGCTTCTTTTAGCAAAGAAAGCATTAAATCATCATAATCAACGTAGAATCTGGTTGTTTCAGTTTCCGTTTTTATCTCAcc                                                          >  2:9058434/1‑140 (MQ=255)

                                                                                gtCTTTTTACCGTGTCTTTTCTGATACTCATTACATGTGTTAATCATTTTTTTGGCCGCTTCATTTATCAAAGAAAGCATTAAATCATCATAATCAACGTAGAATCTGGTTGTTTCAGTTTCCGTTTTTATCTCACCGAt                                                       >  2:650308/1‑140 (MQ=255)

                                                                                gtCTTTTTACCGTGTCTTTTCTGATACTCATTACAGGTGTTAATCATTTTTTTGGCCGCTTCTTTTAGCAAAGAAAGCATTAAATCATCATAATCAACGTAGAATCTGGTTGTTTCAGTTTCCGTTTTTATCTCACCGAt                                                       >  1:5148646/1‑140 (MQ=255)

                                                                                gtCTTTTTACCGTGTCTTTTCTGATACTCATTACAGGTGTTAATCATTTTTTTGGACGCTTCTTTTAGCAAAGAAAGCATTAAATCATCATAATCAACGTAGAATCTGGTTGTTTCAGTTTCCGTTTTTATCTCACCGAt                                                       >  1:6031226/1‑140 (MQ=255)

                                                                                gtCTTTTTACCGTGTCTTTTCTGATACTCATTACAGGTGTTAATCATTTTTATGGCCGCTTCTTTTAGCAAAGAAAGCATTAAATCATCATAATCAACGTATAATCTGGTTGTTTCAGTTTCCGTTTTTATCTCACCGAt                                                       >  2:4929180/1‑140 (MQ=255)

                                                                                 tCTTTTTACCGTGTCTTTTCTGATACTCATTACAGGTGTTAATCATTTTTTTGGCCGCTTCTTTTAGCAAAGAAAGCATTAAATCATCATAATCAACGTAGAATCTGGTTGTTTCAGTTTCCGTTTTTATCTCACCGATg                                                      <  1:177612/140‑1 (MQ=255)

                                                                                 tCTTTTTACCGTGTCTTTTCTGATACTCATTACAGGTGTTAATCATTTTTTTGGCCGCTTCTTTTAGCAAAGAAAGCATTAAATCATCATAATCAACGTAGAATCTGGTTGTTTCAGTTTCCGTTTTTATCTCACCGATg                                                      >  1:8065262/1‑140 (MQ=255)

                                                                                 tCTTTTTACCGTGTCTTTTCTGATACTCATTACAGGTGTTAATCATTTTTTTGGCCGCTTCTTTTAGCAAAGAAAGCATTAAATCATCATAATCAACGTAGAATCTGGTTGTTTCAGTTTCCGTTTTTATCTCACCGATg                                                      >  2:3225015/1‑140 (MQ=255)

                                                                                 tCTTTTGACCGTGTCTTTTCTGATACTCCTTACAGGTGTTAATCATTTTTTTGGCCGCTTCTTTTAGCAAAGAAAGCATTAAATCATCATAATCAACGTAGAATCTGGTTGTTTCAGTTTCCGTTTTTATCTCACCGATg                                                      <  1:9184542/140‑1 (MQ=255)

                                                                                       tACCGTGTCTTTTCTGATACTCATTACAGTTGTTAATCATTTTTTTGGCCGCTTCTTTTAGCAAAGAAAGCATTAAATCATCATAATCAACGTAGAATCTGGTTGGTTCAGTTTCCGTTTTTATCTCACCGATGGCGGCt                                                >  2:8909670/1‑140 (MQ=255)

                                                                                           gtgtCTTTTCTGATACTCATTACAGGTGTTAAGCATTTTTTTGGCCGCTTCTTTTAGCAAAGAAAGCATTAAATCATCATAATCAACGTAGAATCTGGTTGTTTCAGTTTCCGTTTTTATCTCACCGATGGCGGCtcgtt                                            >  2:143636/1‑136 (MQ=255)

                                                                                             gtattttATGATACTCATTACAGGTGTTAATCATTTTTTTGTCCGCTTCTTTTAGCAAATAAAGCATTAAATCATCATAATCATCGTAGAATCTGGTTGTTTAAGTTTCCGTTTTTATCTCAACGAGGGCGGCTATTTCg                                          >  2:4860616/4‑140 (MQ=255)

                                                                                              tCTTTTCTGATACTCATTACAGGTGTTAATCATTTTTTTGGCCGCTTCTTTTAGCAAAGAAAGCATTAAATCATCATAATCAACGTAGAATCTGGTTGTTTAAGTTTCCGTTTTTATCTCACCGATGGCGGCTATTTCgg                                         >  2:9517797/1‑140 (MQ=255)

                                                                                              tCTTTACTGATACTCATAACAGGTGTTAATCATTTTTTTGGCCGCTTCTTATAGCAAAGAAAGCATAAAAACATCATAAACAACCTAGAATCTGGTTGTTTCAGTTTCCGTTTTTATCTCACCGATGGCGGCTATTTCgg                                         <  1:7066948/140‑1 (MQ=255)

                                                                                                   tCTGATACTCATTACAGGTGTTAATCATTTTTTTGGCCGCTTCTTTTAGCAAAGAAAGCATTAAATCATCATAATCAACGTAGAATCTGGTTGTTTCAGTTTCCGTTTTTATCTCACCGATGGCGGCTATTTCGGTGGtt                                    <  1:1504106/140‑1 (MQ=255)

                                                                                                       aTACTCATTACAGGTGTTAATCATTTTTTTGGCCGCTTCTTTTAGCAAAGAAAGCATTAAATCATCATAATCAACGTAGAATCTGGTTGTTTCAGTTTCCGTTTTTATCTCACCGATGGCGGCTATTTCGGTGGTTAAtt                                >  1:6872378/1‑140 (MQ=255)

                                                                                                        tACTCATTACAGGTGTTAATCATTTTTTTGGCCGCTTCTTTTAGCAAAGAAAGCATTAAATCATCATAATCAACGTAGAATCTGGTTGTTTCAGTTTCCGTTTTTATCTCACCGATGGCGGCTATTTCGGTGGTTAAttt                               <  2:4037308/140‑1 (MQ=255)

                                                                                                          cTCATTACAGGTGTTAATCATTTTTTTGGCCGCTTCTTTTAGCAAAGAAAGCATTAAATCATCATAATCAACGTAGAATATGGTTGTTTCAGTTTACGTTTTTATCTCACCGATGGCGGCTATTTCGGTGGTTAATATCa                             >  2:7683579/1‑140 (MQ=255)

                                                                                                          cTCATTACAGGTGTTAATCATTTTTTTGGCCGCTTCTTTTAGCAAAGAAAGCATTAAATCATCATAATAAACGTAGAATCTGGTTGTTTCAGTTTCCGTTTTTATCTCACCGATGGCGGCTATTTCGGTGGTTAATTTCa                             >  1:8614153/1‑140 (MQ=255)

                                                                                                             aTTACAGGTGTTAATCATTTTTTTGGCCGCTTCTTTTAGCAAAGAAAGCATTAAATCATCATAATCAACGTAGAATCTGGTTGTTTCAGTTTCCGTTTTTATCTCACCGATGGCGGCTATTTCGGTGGTTAATTTCAAtt                          >  1:3562084/1‑140 (MQ=255)

                                                                                                             aTTACAGGTGTTAATCATATTTTTGGCCGCTTCTTTTAGCAAAGAAAGCATTAAATCATCATAATCAACGTAGAATCTGGTTGTTTCAGTTTCCGTTTTTATCTCACCGATGGCGGCTATTTCGGTGGTTTATTTCAAtt                          >  2:3772042/1‑140 (MQ=255)

                                                                                                               tACAGGTGTTAATCATTTTTTTGGCCGCTTCTTTTAGCAAAGAAAGCATTAAATCATCATAATCAACGTAGAATCTGGTTGTTTCAGTTTCCGTTTTTATCTCACCGATGGCGGCTATTTCGGTGGTTAATTTCAATTTg                        >  2:5035242/1‑140 (MQ=255)

                                                                                                                  aGGTGTTAATCATTTTTTTGGCCGCTTCTTTTAGCAAAGAAAGCATTAAATCATCATAATCAACGTAGAATCTGGTTGTTTCAGTTTCCGTTTTTATCTCACCGATGGCGGCTATTTCGGTGGTTAATTTCAATTTggcg                     <  1:4813169/140‑1 (MQ=255)

                                                                                                                   ggTGTTAATCATTTTTTTGGCCGCTTCTTTTAGCAAAGAAAGCATTAAATCATCATAATCAACGTAGAATCTGGTTGTTTCAGTTTCCGTTTTTATCTCACCGATGGCGGCTATTTCGGTGGTTAATTTCAATTTggcgg                    <  1:28994/140‑1 (MQ=255)

                                                                                                                    gtgtTAATCATTTTTTTGGCCGCTTCTTTTAGCAAAGAAAGCATTAAATCATCATAATCAACGTAGAATCTGGTTGTTTCAGTTTCCGTTTTTATCTCACCGATGGCGGCTATTTCGGTGGTTAATTTCAATTTggcggc                   >  2:7218331/1‑140 (MQ=255)

                                                                                                                    gtgtTAATCATTTTTTTGGCCGCTTCTTTTAGCAAAGAAAGCATTAAATCATCATAATCAACGTAGAATCTGGTTGTTTCAGTTTCCGTTTTTATCTCACCGATGGCGGCTATTTCGGTGGTTAATTTCAATTTggcggc                   >  1:4251145/1‑140 (MQ=255)

                                                                                                                    gtgtTAATCATTTTTTTGGCCGCTTCTTTTAGCAAAGAAAGCATTAAATCATCATAATCAACGTAGAATCTGGTTGTTTCAGTTTCCGTTTTTATCTCACCGATGGCGGCTATTTCGGTGGTTAATTTCAATTTggcggc                   >  2:3094705/1‑140 (MQ=255)

                                                                                                                    gtgtTAATCATTTTTTTGGCCGCTTCTTTTAGCAAAGAAAGCATTAAATCATCATAATCAACGTAGAATCTGGTTGTTTCAGTTTCCGTTTTTATCTCACCGATGGCGGCTATTTCGGTGGTTAATTTCAATTTggcggc                   <  2:2524742/140‑1 (MQ=255)

                                                                                                                     tgtTAATCATTTTTTTGGCCGCTTCTTTTAGCAAAGAAAGCATTAAATCATCATAATCAACGTAGAATCTGGTTGTTTCAGTTTCCGTTTTTATCTCACCGATGGCGGCTATTTCGGTGGTTAATTTCAATTTGGCGGCa                  <  1:3068436/140‑1 (MQ=255)

                                                                                                                       ttAATCATTTTTTTGGCCGCTTCTTTTAGCAAAGAAAGCATTAAATCATCATAATCAACGTATAATCTGGTTGTTTCAGTTTCCGTTTTTATCTCACCGATGGCGGCTATTTCGGTGGTTAATTTCTATTTGGAGGCATc                >  2:9307301/1‑140 (MQ=255)

                                                                                                                       ttAATCATTTTTTTGGCCGCTTCTTTTAGCAAAGAAAGCATTAAATCATCATAATCAACGTAGAATCTGGTTGTTTCAGTTTCCGTTTTTATCTCACCGATGGCGGCTATTTCGGTGGTTAATTTCAATTTGGCGGCATc                >  1:115127/1‑140 (MQ=255)

                                                                                                                       ttAATCATTTTTTTGGCCGCTTCTTTTAGCAAAGAAAGCATTAAATCATCATAATCAACGTAGAATCTGGTTGTTTCAGTTTCCGTTTTTATCTCACCGATGGCGGCTATTTCGGTGGTTAATTTCAATTTGGCGGCATc                >  1:8880252/1‑140 (MQ=255)

                                                                                                                       ttAATCATTTTTTTGGCCGCTTCTTTTAGCAAAGAAAGCATTAAATCATCATAATCAACGTAGAATCTGGTTGTTTCAGTTTCCGTTTTTATCTCACCGATGGCGGCGATTTCGGTGGTTAATTTCAATTTGGCGGCATc                >  1:5903912/1‑140 (MQ=255)

                                                                                                                       ttAATCATTTTTTTGGCCGCTTCTTTTAGAAAAGAAAGCATTAAATCATCATAATCAACGTAGAATATGGTTGTTTCAGTTTCAGTTTTTATCTCACAGATGGCGGCTATTTCGGTGGTTAATTTCAATTTGTCGGCATc                >  2:1871615/1‑140 (MQ=255)

                                                                                                                        tAATCATTTTTTTGGCCGCTTCTTTTAGCAAAGAAAGCATTAAATCATCATAATCAACGTAGAATCTGGTTGTTTCAGTTTCCGTTTTTATCTCACCGATGGCGGCTATTTCGGTGGTTAATTTCAATTTGGCGGCATCg               <  2:6881225/140‑1 (MQ=255)

                                                                                                                        tAATCATTTTTTTGGCCGCTTCTTTTAGCAAAGAAAGCATTAAATCATCATAATCAACGTAGAATCTGGTTGTTTCAGTTTCCGTTTTTATCTCACCGATGGCGGCTATTTCGGTGGTTAATTTCAATTTGGCGGCATCg               <  1:9431648/140‑1 (MQ=255)

                                                                                                                         aaTCATTTTTTTGGCCGCTTCTTTTAGCAAAGAAAGCATTAAATCATCATAATCAACGTAGAATCTGGTTGTTTCAGTTTCCGTTTTTATCTCACCGATGGCGTCTATTTCGGTGGTTAATTTCTATTTGTCGGCATCGa              >  2:8517963/1‑140 (MQ=255)

                                                                                                                         aaTCATTTTTTTGGCCGCTTCTTTTAGCAAAGAAAGCATTAAATCATCATAATCAACGTAGAATCTGGTTGTTTCAGTTTCCGTTTTTATCTCACCGATGGCGGCTATTTCGGTGGTTAATTTCAATTTGGCGGCATCGa              <  2:4539609/140‑1 (MQ=255)

                                                                                                                         aaTCATTTTTTTGGCCGCTTCTTTTAGCAAAGAAAGCATTAAATCATCATAATCAACGTAGAATCTGGTTGTTTCAGTTTCCGTTTTTATCTCACCGATGGCGGCTATTTCGGTGGTTAATTTCAATTTGGCGGCATCGa              <  2:4125418/140‑1 (MQ=255)

                                                                                                                              tttttttGGCCGCTTCTTTTAGCAAAGAAAGCATTAAATCATCATAATCa                                                                                                   <  2:8158728/50‑1 (MQ=255)

                                                                                                                              tttttttGGCCGCTTCTTTTAGCAAAGAAAGCATTAAATCATCATAATCa                                                                                                   >  1:8158728/1‑50 (MQ=255)

                                                                                                                               ttttttGGTCGCTTCTTTTAGCAAAGAAGGCATTAAATCATCATAATCAACGTAGAATCTGGTTGTTTCAGTTTCCGTTTTTATCTCACCGATGGCGGCTATTTCGGTGGTTAATTTCAATTTGGCGGCATCGATATCtt        >  2:5993642/1‑140 (MQ=255)

                                                                                                                                    tGGCCGCTTCTTTTAGCAAAGAAAGCATTAAATCATCATAATCAACGTAGAATCTGGTTGTTTCAGTTTCCGTTTTTATCTCACCGATGGCGGCTATTTCGGTGGTTAATTTCAATTTGGCGGCATCGATATCTTTATTc   <  2:4735862/140‑1 (MQ=255)

                                                                                                                                    tGGCCGCTTCTTTTAGCAAAGAAAGCATTAAATCATCATAATCAACGTAGAATCTGGTTGTTTCAGTTTCCGTTTTTATCTCACCGATGGCGGCTATTTCGGTGGTTAATTTCAATTTGGCGGCATCGATATCTTTATTc   <  1:4946953/140‑1 (MQ=255)

GTTTTTAAATGTGATAAACCTTATACCTTGAGTACATGGAGAGAATAATCGCTTATCAGACTTCAGGTACCTCAAAGAGTGTCTTTTTACCGTGTCTTTTCTGATACTCATTACAGGTGTTAATCATTTTTTTGGCTGCTTCTTTTAGCAAAGAAAGCATTAAATCATCATAATCAACGTAGAATCTGGTTGTTTCAGTTTCCGTTTTTATCTCACCGATGGCGGCTATTTCGGTGGTTAATTTCAATTTGGCGGCATCGATATCTTTATTCG  >  NZ_CP025268/1236526‑1236798

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`