BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP025268	1,423,978	A→G	163 (TAA→TAG) S51S (AGT→AGC)	sieB → ydaF ←	superinfection exclusion B family protein YdaF family protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP025268	1,423,978	0	A	G	99.8%	1767.7 / NA	514	163 (TAA→TAG) S51S (AGT→AGC)	sieB ydaF	superinfection exclusion B family protein YdaF family protein
Reads supporting (aligned to +/- strand): ref base A (0/0); major base G (238/275); minor base T (1/0); total (239/275)
Fisher's exact test for biased strand distribution p-value = 4.65e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 514 total aligned reads displayed.

ATGAAAAAATTAGAACGGAAGGGCATCATTCAACATCAGAGTGATAGCGCAAACTGTTCTTATTATCTCGTCACCGAAAAATACTCCCATTTTATGAAGTTATTCTGGAACAGCAGGAGTAGACGTTTTAATCGTTAACTTACTGTGTGCTTCTCCAACCATCGGCGCGCACCAGTTTCGGTTTTAAATGTTTTGCTTTTGGTATACGTCATGGCAGTGAACGTTCCATCCTGGTTGGGGAACACGCCGCACACCAGGGATTCGTTGTTGCC  >  NZ_CP025268/1423841‑1424112

aTGAAAAAATTAGAACGGAAGGGCATCATTCAACATCAGAGTGATAGCGCAAACTGTTCTTATTATCTCGTCACCGAAAAATACTCCCATTTTATGAAGTTATTCTGGAACAGCAGGAGTAGACGTTTTAATCGTTAGCt                                                                                                                                      <  2:3294593/140‑1 (MQ=255)

  gAAAAAATTAGAACGGAAGGGCATCATTCAACATCAGAGTGATAGCGCAAACTGTTCTTATTATCTCGTCACCGAAAAATACTCCCATTTTATGAAGTTATTCTGGAACAGCAGGAGTAGACGTTTTAATCGTTAGCTTa                                                                                                                                    <  2:6683418/140‑1 (MQ=255)

   aaaaaaTTAGAACGGAAGGGCATCATTCAACATCAGAGTGATAGCGCAAACTGTTCTTATTATCTCGTCACCGAAAAATACTCCCATTTTATGAAGTTATTCTGGACCAGCAGGAGTAGACGTTTTAATCGTTAGCTTAc                                                                                                                                   >  1:6018694/1‑140 (MQ=255)

        aTTAGAACGGAAGGGCATCATTCAACATCAGAGTGATAGCGCAAACTGTTCTTATTATCTCGTCACCGAAAAATACTCCCATTTTATGAAGTTATTCTGGAACAGCAGGAGTAGACGTTTTAATCGTTAGCTTACtgtgt                                                                                                                              >  1:537413/1‑140 (MQ=255)

        aTTAGAACGGAAGGGCATCATTCAACATCAGAGTGATAGCGCAAACTGTTCTTATTATCTCGTCACCGAAAAATACTCCCATTTTATGAAGTTATTCTGGAACAGCAGGAGTAGACGTTTTAATCGTTAGCTTACtgtgt                                                                                                                              <  2:8325620/140‑1 (MQ=255)

         ttAGAACGGAAGGGCATCATTCAACATCAGAGTGATAGCGCAAACTGTTCTTATTATCTCGTCACCGAAAAATACTCCCATTTTATGAAGTTATTCTGGAACAGCAGGAGTAGACGTTTTAATCGTTAGCTTACtgtgtg                                                                                                                             <  2:1694360/140‑1 (MQ=255)

         atAGATCGGAAGGGCATCATTCAACATCAGAGTGATAGCGCAAACTGTTCTTATTATCTCGTCACCGAAAAATACTCCCTTTTTATGAAGTTATTCTGGAACAGCAGGAGTAGAAGTTTTAATCGTTAGCTTACtgtgtg                                                                                                                             <  1:7983148/139‑1 (MQ=255)

              aCGGAAGGGCATCATTCAACATCAGAGTGATAGCGCAAACTGTTCTTATTATCTCATCACCGAAAAATACTCCCATTTTATGAAGTTATTCTGGAACAGCAGGAGTAGACGTTTTAATCGTTAGCTTACTGTGTGCTtct                                                                                                                        <  2:8237774/140‑1 (MQ=255)

                ggAAGGGCATCATTCAACATCAGAGTGATAGCGCAAACTGTTCTTATTATCTCGTCACCGAAAAATACTCCCATTTTATGAAGTTATTCTGGAACAGCAGGAGTAGACGTTTTAATCGTTAGCTTACTGTGTGCTTCTcc                                                                                                                      <  1:6485181/140‑1 (MQ=255)

                cgAAGGCCATCATTCAACATCAGAGTGATAGCGAAAACTGTTCGTATTATCTCGACACCGAAAAATACGACCAATTTATGAAGTTATTCTGGAACAGCAGGTGTAGACGTTTTAATCTTTAGCTTACTTTGTGCTTCTcc                                                                                                                      <  2:7632015/139‑1 (MQ=255)

                  aaGGGCATCATTCAACATCATAGAGATAGCGCAAACTGTTGTTATTATCTCGTCACCGAAAAATACTCAAATTTTATTAAGTTATTCTGGAACAGCTGGAGTTGACGATTTATTCGTTAGCTTACTGTGTGCTTCTcctt                                                                                                                    >  2:8134561/1‑138 (MQ=255)

                      gCATCATTCAACATCAGAGTGATAGCGCAAACTGTTCTTATTATCTCGTCACCGAAAAATACTCCCATTTTATGAAGTTATTCTGGAACAGCAGGAGTAGACGTTTTAATCGTTAGCTTACTGTGTGCTTCTCCAACCAt                                                                                                                <  2:8665670/140‑1 (MQ=255)

                       catcatTCAACATCAGAGTCATCCCGCAAACTGTTCGTATTATCTCGTCACCGAAAAATACTCCCAGTTTATGAAGTTATTCTGGAACAGCAGGAGTAGACGTTGTAATCGTTAGCTTACTGTGTGCTGCTCCAACCagc                                                                                                               >  2:5987026/1‑138 (MQ=255)

                        atcatTCAACATCAGAGTGATAGCGCAAACTGTTCTTATTATCTCGTCACCGAAAAATACTCCCATTTTATGAAGTTATTCTGGAACAGCAGGAGTAGACGTTTTAATCGTTAGCTTACTGTGTGCTTCTCCAACCATCg                                                                                                              <  2:1471410/140‑1 (MQ=255)

                         tcatTCAACATCAGAGTGATAGCGCAAACTGTTCTTATTATCTCGTCACCGAAAAATACTCCCATTTTATGAAGTTATTCTGGAACAGCAGGAGTAGACGTTTTAATCGTTAGCTTACTGTGTGCTTCTCCAACCATCgg                                                                                                             >  2:296260/1‑140 (MQ=255)

                           atTCTACATCAGAGTGATAGCGCAAACTGTTCTTATTATCTCGTCACCGAAAAATACTCCCATTTTATGAAGTTATTCTGGAACAGCAGGAGTAGACGTTTTAATCGTTAGCTTACTGTGTGCTTCTCCAACCATCGgcg                                                                                                           <  2:5505800/140‑1 (MQ=255)

                           atTCAACATCAGAGTGATAGCGCAAACTGTTCTTATTATCTCGTCACCGAAAAATACTCCCATTTTATGAAGTTATTCTGGAACAGCAGGAGTAGACGTTTTAATCGTTAGCTTACTGTGTGCTTCTCCAACCATCGgcg                                                                                                           <  2:1616608/140‑1 (MQ=255)

                           atTCAACATCAGAGTGATAGCGCAAACTGTTCTTATTATCTCGTCACCGAAAAATACTCCCATTTTATGAAGTTATTCTGGAACAGCAGGAGTAGACGTTTTAATCGTTAGCTTACTGTGTGCTTCTCCAACCATCGgcg                                                                                                           <  1:4194952/140‑1 (MQ=255)

                               aaCATCAGAGTGATAGCGCAAACTGTTCTTATTATCTCGTCACCGAAAAATACTCCCATTTTATGAAGTTATTCTGGAACAGCAGGAGTAGACGTTTTAATCGTTAGCTTACTGTGTGCTTCTCCAACCATCGGCGCGCa                                                                                                       >  1:1695055/1‑140 (MQ=255)

                               aaCATCAGAGTGATAGCGCAAACTGTTCTTATTATCTCGTCACAGAAAAATACTCCCATTTTATGAAGGTATTCTGGAACAGCAGGAGTAGACGTTTTAATCGTTATCTTACTGTGTGCTTCTACAACCCTCGGCGCGCa                                                                                                       >  1:7191789/1‑140 (MQ=255)

                                    cAGAGTGATAGCGCAAACTGTTCTTATTATCTCGTCACCGAAAAATACTCCCATTTTATGAAGTTATTCTGGACCAGCAGGAGTAGACGTTTTAATCGTTAGATTACTGTGAGCTACTCCAACCATCGGCGCGCACCAGt                                                                                                  >  2:7813580/1‑140 (MQ=255)

                                    cAGAGTGATAGCGCAAACTGTTCTTATTATCTCGTCACCGAAAAATACTCCCATTTTATGAAGTTATTCTGGAACAGCAGGAGTAGACGTTTTAATCGTTAGCTTACTGTGTGCTTCTCCAACCATCGGCGCGCACCAGt                                                                                                  >  2:6120097/1‑140 (MQ=255)

                                    cAGAGTGATAGCGCAAACTGTTCTTATTATCTCGTCACCGAAAAATACTCCCATTTTATGAAGTTATTCTGGAACAGCAGGAGTAGACGTTTTAATCGTTAGCTTACTGTGTGCTTCTCCAACCATCGGCGCGCACCAGt                                                                                                  >  2:2483273/1‑140 (MQ=255)

                                    cAGAGTGATAGCGCAAACTGTTCTTATTATCTCGTCACCGAAAAATACTCCCATTTTATGAAGTTATTCTGGAACAGCAGGAGTAGACGTTTTAATCGTTAGCTTACTGTGTGCTTCTCCAACCATCGGCGCGCACCAGt                                                                                                  >  1:6235975/1‑140 (MQ=255)

                                    cAGAGTGATAGCGCAAACTGTTCTTATTATCTCGTCACCGAAAAATACTCCCATTTTATGAAGTTATTCTGGAACAGCAGGAGTAGACGTTTTAATCGTTAGCTTACTGTGTGCTTCTCCAACCATCGGCGCGCACCAGt                                                                                                  >  1:6643245/1‑140 (MQ=255)

                                    cAGAGTGATAGCGCAAACTGTTCTTATTATCTCGTCACCGAAAAATACTCCCATTTTATGAAGTTATTCTGGAACAGCAGGAGTAGACGTTTTAATCGTTAGCTTACTGTGTGCTTCTCCAACCATCGGCGCGCACCAGt                                                                                                  >  1:1514964/1‑140 (MQ=255)

                                      gagTGATAGCGCAAACTGTTCTTATTATCTCGTCACCGAAAAATACTCCCATTTTATGAAGTTATTCTGGAACAGCAGGAGTAGACGTTTTAATCGTTAGCTTACTGTGTGCTTCTCCAACCATCGGCGCGCACCAGttt                                                                                                <  1:2928498/140‑1 (MQ=255)

                                      gagTGATAGCGCAAACTGTTCTTATTATCTCGTCACCGAAAAATACTCCCATTTTATGAAGTTATTCTGGAACAGCAGGAGTAGACGTTTTAATCGTTAGCTTACTGTGTGCTTCTCCAACCATCGGCGCGCACCAGttt                                                                                                >  2:3516308/1‑140 (MQ=255)

                                       agTGATAGCGCAAACTGTTCTTATTATCTCGTCACCGAAAAATACTCCCATTTTATGAAGTTATTCTGGAACAGCAGGAGTAGACGTTTTAATCGTTAGCTTACTGTGTGCTTCTCCAACCATCGGCGCGCACCAGTTTc                                                                                               >  2:6906746/1‑140 (MQ=255)

                                       agTGATAGCGCAAACTGTTCTTATTATCTCGTCACCGAAAAATACTCCCATTTTATGAAGTTATTCTGGAACAGCAGGAGTAGACGTTTTAATCGTTAGCTTACTGTGTGCTTCTCCAACCATCGGCGCGCACCAGTTTc                                                                                               >  2:7056774/1‑140 (MQ=255)

                                       agTGATAGCGCAAACTGTTCTTATTATCTCGTCACCGAAAAATACTCCCATTTTATGAAGTTATTCTGGAACAGCAGGAGTAGACGTTTTAATCGTTAGCTTACTGTGTGCTTCTCCAACCATCGGCGCGCACCAGTTTc                                                                                               >  1:7503241/1‑140 (MQ=255)

                                       agTGATAGCGCAAACTGTTCTTATTATCTCGTCACCGAAAAATACTCCCATTTTATGAAGTTATTCTGGAACAGCAGGAGTAGACGTTTTAATCGTTAGCTTACTGTGTGCTTCTCCAACCATCGGCGCGCACCAGTTTc                                                                                               >  1:9407744/1‑140 (MQ=255)

                                       agTGATAGCGCAAACTGTTCTTATTATCTCGTCACCGAAAAAGACTCCCATTTTATGAAGTTATTCTGGAACAGCAGGAGTAGACGTTTTAATCGTTAGCTTACTGTGTGCTTCTCCAACCATCGGCGCGCACCAGTTTc                                                                                               <  2:7206456/140‑1 (MQ=255)

                                           aTAGCGCAAACTGTTCTTATTATCTCGTCACCGAAAAATACTCCCATTTTATGAAGTTATTCTGGAACAGCAGGAGTAGACGTTTTAATCGTTAGCTTACTGTGTGCTTCTCCAACCATCGGCGCGCACCAGTTTCGGtt                                                                                           >  1:4569207/1‑140 (MQ=255)

                                           aTAGCGCAAACTGTTCTTATTATCTCGTCACCGAAAAATACTCCCATTTTATGAAGTTATTCTGGAACAGCAGGAGTAGACGTTTTAATCGTTAGCTTACTGTGTGCTTCTCCAACCATCGGCGCGCACCAGTTTCGGtt                                                                                           >  1:1816801/1‑140 (MQ=255)

                                           aTAGCGCAAACTGTTCTTATTATCTCGTCACCGAAAAATACTCCCATTTTATGAAGTTATTCTGGAACAGCAGGAGTAGACGTTTTAATCGTTAGCTTACTGTGTGCTTCTCCAACCATCGGCGCGCACCAGTGTCGGtt                                                                                           >  1:2892484/1‑140 (MQ=255)

                                               cgcAAACTGTTCTTATTATCTCGTCACCGAAAATTACTCCCATTTTATGAAGTTATTCTGGAACAGCAGGAGTAGACGTTTTAATCGTTAGCTTACTGTGTGCTTCTCCAACCATCGGCGCGCACCAGTTTCGGTTTTaa                                                                                       >  2:2277021/1‑140 (MQ=255)

                                                   aaCTGTTCTTATTATCTCGTCACCGAAAAATACTCCCATTTTATGAAGTTATTCTGGAACAGCAGGAGTAGACGTTTTAATCGTTAGCTTACTGTGTGCTTCTCCAACCATCGGCGCGCACCAGTTTCGGTTTTAAATGt                                                                                   >  2:2590925/1‑140 (MQ=255)

                                                   aaCTGTTCTTATTATCTCGTCACCGAAAAATACTCCCATTTTATGAAGTTATTCTGGAACAGCAGGAGTAGACGTTTTAATCGTTAGCTTACTGTGTGCTTCTCCAACCATCGGCGCGCACCAGTTTCGGTTTTAAATGt                                                                                   >  2:4413935/1‑140 (MQ=255)

                                                    aCTGTTCTTATTATCTCGTCACCGAAAAATACTCCCATTTTATGAAGTTATTCTGGAACAGCAGGAGTAGACGTTTTAATCGTTAGCTTACTGTGTGCTTCTCCAACCATCGGCGCGCACCAGTTTCGGTTTTAAATGtt                                                                                  <  1:5310706/140‑1 (MQ=255)

                                                     ctcttCTTATTATCTCGACACCGAAAAATACTCCCATTTTATGAAGATATTCTGCAACAGCAGGAGTAGACGTTTTAATCGTTAGCTTACTGTGTGCTTCTCCAACCATCGGCGCGCACCAGTTTCGGTTTTAAATGttt                                                                                 <  2:133480/137‑1 (MQ=255)

                                                     ctcttCTTATACTCTCCTCACCGCAAAATACTCCCAATTTATGACGTTAGTCTGGAACAGCAGGAGTAGACGTTTTAATCGTTAGCTGACTGTCTGCTTCTCCAACCATCGGCGCGCACCAGTTTCGGATTTAAATGgtt                                                                                 <  2:7693676/137‑1 (MQ=255)

                                                      tGTTCTTATTATCTCGTCACCGAAAAATACTCCCATTTTATGAAGTTATTCTGGAACAGCAGGAGTAGACGTTTTAATCGTTAGCTTACTGTGTGCTTCTCCAACCATCGGCGCGCACCAGTTTCGGTTTTAAATGtttt                                                                                <  2:403294/140‑1 (MQ=255)

                                                        ttCTTATTATCTCGTCACCGAAAAATACTCCCATTTTATGAAGTTATTCTGGAACAGCAGGAGTAGACGTTTTAATCGTTAGCTTACTGTGTGCTTCTCCAACCATCGGCGCGCACCAGTGTCGGTTTTAAATGTTTTGc                                                                              >  1:761631/1‑140 (MQ=255)

                                                        ttCTTATTATCTCGTCACCGAAAAATACTACCATTTTAAGAAGTTATACTGGAACAGCAGGATTAGACGTTTTAATCGTTAGCTTACTGTGTGCTTCTCAAACCATCGGCGAGCACCAGTTTCGGTTTTAAATTTTATGc                                                                              >  2:8779300/1‑140 (MQ=255)

                                                          cTTATTATCTCGTCACCGAAAAATACTCCCATTTTATGAAGTTATTCTGGAACAGCAGGAGTAGACGTTTTAATCGTTAGCTTACTGTGTGCTTCTCCAACCATCGGCGCGCACCAGTTTCGGTTTTAAATGTTTTGCtt                                                                            <  1:1279173/140‑1 (MQ=255)

                                                          cTTATTATCTCGTCACCGAAAAATACTCCCATTTTATGAAGTTATTCTGGAACAGCAGGAGTAGACGTTTTAATCGTTAGCTTACTGTGTGCTTCTCCAACCATCGGCGCGCACCAGTTTCGGTTTTAAATGTATTGCtt                                                                            <  2:1110737/140‑1 (MQ=255)

                                                           ttattaTCTCGTCACCGAAAAATACTCCCATTTTATGAAGTTATTCTGGAACAGCAGGAGTAGACGTTTTAATCGTTAGCTTACTGTGTGCTTCTCCAACCATCGGCGCGCACCAGTTTCGGTTTTAAATGTTTTGCttt                                                                           <  2:3065809/140‑1 (MQ=255)

                                                           ttattaTCTCGTCACCGAAAAATACTCCCATTTTATGAAGTTATTCTGGAACAGCAGGAGTAGACGTTTTAATCGTTAGCTTACTGTGTGCTTCTCCAACCATCGGCGCGCACCAGTTTCGGTTTTAAATGTTTTGCttt                                                                           <  1:909611/140‑1 (MQ=255)

                                                             attatCTCGTCACCGAAAAATACTCCCATTTTATGAAGTTATTCTGGAACAGCAGGAGTAGACGTTTTAATCGTTAGCTTACTGTGTGCTTCTCCAACCATCGGCGCGCACCAGTTTCGGTTTTAAATGTTTTGCTTTTg                                                                         >  1:9444789/1‑140 (MQ=255)

                                                             attatCTCGTCACCGAAAAANACTCCCATTTTATGAAGTTATTCTGGAACAGCAGGAGTAGACGTTTTAATCGTTAGCTTACTGTGTGCTTCTCCAACCATCGGCNCGCACCANTTTCGGTTTTAAATGTTTTGCTTTTg                                                                         >  1:5526411/1‑140 (MQ=255)

                                                                atCTCGTCACCGAAAACTACTCCCATTTTATGAAGTTATTCTGGAACAGCAGGAGTAGACGTTTTAATCGTTAGCTTACTGTGTGCTTCCCCAACCATCGGCGCGCACCAGTTTCGGTTTTAAATGTTTTGCTTTTGGta                                                                      <  2:2709058/140‑1 (MQ=255)

                                                                atCTCGTCACCGAAAAATACTCCCATTTTATGAAGTTATTCTGGAACAGCAGGAGTAGACGTTTTAATCGTTAGCTTACTGTGTGCTTCTCCAACCATCGGCGCGCACCAGTTTCGGTTTTAAATGTTTTGCTTTTGGta                                                                      <  1:2002815/140‑1 (MQ=255)

                                                                atCTCGTCACCGAAAAATACTCCCATTTTATGAAGTTATTCTGGAACAGCAGGAGTAGACGTTTTAATCGTTAGCTTACTGTGTGCTTCTCCAACCATCGGCGCGCACCAGTTTCGGTTTTAAATGTTTTGCTTTTGGta                                                                      <  2:2842373/140‑1 (MQ=255)

                                                                atCTCGTCACCGAAAAATACTCCCATTTTATGAAGTTATTCTGGAACAGCAGGAGTAGACGTTTTAATCGTTAGCTTACTGTGTGCTTCTCCAACCATCGGCGCGCACCAGTTTCGGTTTTAAATGTTTTGCTTTTGGta                                                                      <  1:3434013/140‑1 (MQ=255)

                                                                  ctcGTCACCGAAAAATACTCCCATTTTATGAAGTTATTCTGGAACAGCAGGAGTAGACGTTTTCATCGTTAGCTTACTGTGTGCTTCTCCAACCATCGGCGCGCACCAGTTGCGGTTTTACCTGTTTGGCTTTTGGtata                                                                    >  2:9369380/1‑140 (MQ=255)

                                                                  ctcGTCACCGAAAAATACTCCCATTTTATGAAGTTATTCTGGAACAGCAGGAGTAGACGTTTTAATCGTTAGCTTACTGTGTGCTTCTCCAACCATCGGCGCGCACCAGTTTCGGTTTTAATTGTTTTGCTTTTGGtata                                                                    >  2:6224674/1‑140 (MQ=255)

                                                                  ctcGTCACCGAAAAATACTCCCATTTTATGAAGTTATTCTGGAACAGCAGGAGTAGACGTTTTAATCGTTAGCTTACTGTGTGCTTCTCCAACCATCGGCGCGCACCAGTTTCGGTTTTAAATGTTTTGCTTTTGGtata                                                                    >  1:1003460/1‑140 (MQ=255)

                                                                  ctcGTCACCGAAAAATACTCCCATTTTATGAAGTTATTCTGGAACAGCAGGAGTAGACGTTTTAATCGTTAGCTTACTGTGTGCTTCTCCAACCATCGGCGCGCACCAGTTTCGGTTTTAAATGTTTTGCTTTTGGtata                                                                    >  1:8647328/1‑140 (MQ=255)

                                                                  ctcGTCACCGAAAAATACTCCCATTTTATGAAGTTATTCTGGAACAGCAGGAGTAGACGTTTTAATCGTTAGCTTACTGTGTGCTTCTCCAACCATCGGCGCGCACCAGTTTCGGTTTTAAATGTTTTGCTTTTGGtata                                                                    >  2:3183913/1‑140 (MQ=255)

                                                                  ctcGTCACCGAAAAATACTCCCATTTTATGAAGTTATTCTGGAACAGCAGGAGTAGACGTTTTAATCGTTAGCTTACTGTGTGCTTCTCCAACCATCGGCGCGCACCAGTGTCGGTTTTAAATGTTTTGCTTTTGGtata                                                                    >  2:6356126/1‑140 (MQ=255)

                                                                     gTCACCGAAAAATACTCCCATTTTATGAAGTTATTCTGGAACAGCAGGAGTAGACGTTTTAATCGTTAGCTTACTGTGTGCTTCTCCAACCATCGGCGCGCACCAGTTTCGGTTTTAAATGTTTTGCTTTTGGTATACGt                                                                 >  2:6548812/1‑140 (MQ=255)

                                                                       cACCGAAAAATACTCCCATTTTATGAAGTTATTCTGGAACAGCAGGAGTAGACGTTTTAATCGTTAGCTTACTGTGTGCTTCTCCAACCATCGGCGCGCACCAGTTTCGGTTTTAAATGTTTTGCTTTTGGTATACGTCa                                                               <  1:3871585/140‑1 (MQ=255)

                                                                       cACCGAAAAATACTCCCATTTTATGAAGTTATTCTGGAACAGCAGGAGTAGACGTTTTAATCGTTAGCTTACTGTGTGCTTCTCCAACCATCGGCGCGCACCAGTTTCGGTTTTAAATGTTTTGCTTTTGGTATACGTCa                                                               >  2:7273292/1‑140 (MQ=255)

                                                                       cACCGAAAAATACTCCCATTTTATGAAGTTATTCTGGAACAGCAGGAGTAGACGTTTTAATCGTTAGCTTACTGTGTGCTTCTCCAACCATCGGCGCGCACCAGTTTCGGTTTTAAATGTTTTGCTTTTGGTATACGTCa                                                               <  1:1098382/140‑1 (MQ=255)

                                                                       cACCGAAAAATACTCCCAGTTTATGAAGTTATTCTGGAACAGCTGGAGTAGACGTTGTAATCGTTAGGTTACTGTGTGCTTCTCCAAACATGGTCGGGCACCAGTTTCGGTTTTAACTGTTTTGCTTTGGGTATACGtga                                                               >  2:5777666/1‑138 (MQ=255)

                                                                         ccGAAAAATACTCCCATTTTATGAAGTTATTCTGGAACAGCAGGAGTAGACGTTTTAATCGTTAGCTTACTGTGTGCTTCTCCAACCATCGGCGCGCACCAGTTTCGGTTTTAAATGTTTTGCTTTTGGTATACGTCATg                                                             <  2:9007802/140‑1 (MQ=255)

                                                                           gAAAAATACGCCCATTTTATGAAGTTATTCTGGAACAGCAGGAGTAGACGTTTTAATCGTGAGCTTACTGTGTGCTTCTCCAACCATCGGCGCGCACCAGTTTCGGTTTTAAATGTTTTGCTTTTGGTATACGTCATGGc                                                           <  2:9171957/140‑1 (MQ=255)

                                                                               aaTACTCCCATTTTATGAAGTTATTCTGGAACAGCAGGAGTAGACGTTTTAATCGTTAGCTTACTGTGTGCTTCTCCAACCATCGGCGCGCACCAGTTTCGGTTTTAAATGTTTTGCTTTTGGTATACGTCATGGCAGTg                                                       >  1:673159/1‑140 (MQ=255)

                                                                                aTACTCCCATTTTATGAAGTTATTCTGGAACAGCAGGAGTAGACGTTTTAATCGTTAGCTTACTGTGTGCTTCTCCAACCATCGGCGCGCACCAGTTTCGGTTTTAAATGTTTTGCTTTTGGTATACGTCATGGCAGTGa                                                      <  1:2640339/140‑1 (MQ=255)

                                                                                aTACTCCCATTTTATGAAGTTATTCTGGAACAGCAGGAGTAGACGTTTTAATCGTTAGCTTACTGTGTGCTTCTCCAACCATCGGCGCGCACCAGTTTCGGTTTTAAATGTTTTGCTTTTGGTATACGTCATGGCAGTGa                                                      <  1:3802886/140‑1 (MQ=255)

                                                                                 tACTCCCATTTTATGAAGTTATTCTGGAACAGCAGGAGTAGACGTTTTAATCGTTAGCTTACTGTGTGCTTCTCCAACCATCGGCGCGCACCAGTTTCGGTTTTAAATGTTTTGCTTTTGGTATACGTCATGGCAGTGaa                                                     <  2:5752062/140‑1 (MQ=255)

                                                                                    tCCCATTTTATGAAGTTATTCTGGAACAGCAGGAGTAGACGTTTTAATCGTTAGCTTACTGTGTGCTTCTCCAACCATCGGCGCGCACCAGTTTCGGTTTTAAATGTTTTGCTTTTGGTATACGTCATGGCAGTGAACGt                                                  >  2:3678610/1‑140 (MQ=255)

                                                                                     cccATTTTATGAAGTTATTCTGGAACAGCAGGAGTAGACGTTTTAATCGTTAGCTTACTGTGTGCTTCTCCAACCATCGGCGCGCACCAGTTTCGGTTTTAAATGTTTTGCTTTTGGTATACGTCATGGCAGTGAACGtt                                                 <  2:4790092/140‑1 (MQ=255)

                                                                                         ttttATGAAGTTATTCTGGAACAGCAGGAGTAGACGTTTTAATCGTTAGCTTACTGTGTGCTTCTCCAACCATCGGCGCGCACCAGTTTCGGTTTTAAATGTTTTGCTTTTGGTATACGTCATGGCAGTGAACGTTCCAt                                             >  1:4918098/1‑140 (MQ=255)

                                                                                            tATGAAGTTATTCTGGAACAGCAGGAGTAGACGTTTTAATCGTTAGCTTACTGTGTGCTTCTCCAACCATCGGCGCGCACCAGTTTCGGTTTTAAATGTTTTGCTTTTGGTATACGTCATGGCAGTGAACGTTCCATCCt                                          >  1:8594463/1‑140 (MQ=255)

                                                                                                     aTTCTGGAACAGCAGGAGTAGACGTTTTAATCGTTAGCTTACTGTGTGCTTCTCCAACCATCGGCGCGCACCAGTTTCGGTTTTAAATGTTTTGCTTTTGGTATACGTCATGGCAGTGAACGTTCCATCCTGGTTGGGGa                                 >  2:8588183/1‑140 (MQ=255)

                                                                                                     aTTCTGGAACAGCAGGAGTAGACGTTTTAATCGTTAGCTTACTGTGTGCTTCTCCAACCATCGGCGCGCACCAGTTTCGGTTTTAAATGTTTTGCTTTTGGTATACGTCATGGCAGTGAACGTTCCATCCTGGTTGGGGa                                 >  2:7434815/1‑140 (MQ=255)

                                                                                                     aTTCTGGAACAGCAGGAGTAGACGTTTTAATCGTTAGCTTACTGTGTGCTTCTCCAACCATCGGCGCGCACCAGTTTCGGTTTTAAATGTTTTGCTTTTGGTATACGTCATGGCAGTGAACGTTCCATCCTGGTTGGGGa                                 <  1:8165353/140‑1 (MQ=255)

                                                                                                       tCTGGAACAGCAGGAGTAGACGTTTTAATCGTTAGCTTACTGTGTGCTTCTCCAACCATCGGCGCGCACCAGTTTCGGTTTTAAATGTTTTGCTTTTGGTATACGTCATGGCAGTGAACGTTCCATCCTGGTTGGGGAac                               <  1:4014641/140‑1 (MQ=255)

                                                                                                          ggAACAGCAGGAGTAGACGTTTTAATCGTTAGCTTACTGTGTGCTTCTCCAACCATCGGCGCGCACCAGTTTCGGTTTTATATGTTTTGCTTTTGGTATACGTCATGGCAGTGAACGTTCCATCCTGGTTGGGGAACAcg                            <  2:887089/140‑1 (MQ=255)

                                                                                                          ggAACAGCAGGAGTAGACGTTTTAATCGTTAGCTTACTGTGTGCTTCTCCAACCATCGGCGCGCACCAGTTTCGGTTTTAAATGTTTTGCTTTTGGTATACGTCATGGCAGTGAACGTTCCATCCTGGTTGGGGAACAcg                            <  1:4684898/140‑1 (MQ=255)

                                                                                                            aaCAGCAGGAGTAGACGTTTTAATCGTTAGCTTACTGTGTGCTTCTCCAACCATCGGCGCGCACCAGTTTCGGTTTTAAATGTTTTGCTTTTGGTATACGTCATGGCAGTGAACGTTCCATCCTGGTTGGGGAACAcgcc                          <  1:5147929/140‑1 (MQ=255)

                                                                                                             aCAGCAGGCGTAGTCGTTTGTCTCGTTAGCTAACGGTCTCCTTCACCATCCCGCTGCGCGGACCAGTTTCGGTTGAAAATGTTTTGCTAGGTGTAGACGTCATGGCAGTGAACGTTCCATCCTGTTTCTGGAACTagccg                         <  2:8483695/140‑1 (MQ=255)

                                                                                                             aCAGCAGGAGTAGACGTTTTAATCGTTAGCTTACTGTGTGCTTCTCCAACCATCGGCGCGCACCAGTTTCGGTTTTAAATGTTTTGCTTTTGGTATACGTCATGGCAGTGAACGTTCCATCCTGGTTGGGGAACAcgccg                         <  1:2273240/140‑1 (MQ=255)

                                                                                                             aCAGCAGGAGTAGACGTTTTAATCGTTAGCTTACTGTGTGCTTCTCCAACCATCGGCGCGCACCAGTTTCGGTTTTAAATGTTTTGCTTTTGGTATACGTCATGGCAGTGAACGTTCCATCCTGGTTGGGGAACAcgccg                         <  1:4049836/140‑1 (MQ=255)

                                                                                                             aCAGCAGGAGTAGACGTTTTAATCGTTAGCTTACTGTGTGCTTCTCCAACCATCGGCGCGCACCAGTTTCGGTTTTAAATGTTTTGCTTTTGGTATACGTCATGGCAGTGAACGTTCCATCCTGGTTGGGGAACAcgccg                         <  1:3712680/140‑1 (MQ=255)

                                                                                                               agcagGAGTAGACGTTTTAATCGTTAGCTTACTGTGTGCTTCTCCAACCATCGGCGCGCACCAGTTTCGGTTTTAAATGTTTTGCTTTTGGTATACGTCCTGGCAGTGAACGTTCCATCCTGGTTGGGGACCACGCCGca                       >  2:1783585/1‑140 (MQ=255)

                                                                                                               agcagGAGTAGACGTTTTAATCGTTAGCTTACTGTGTGCTTCTCCAACCATCGGCGCGCACCAGTTTCGGTTTTAAATGTTTTGCTTTTGGTATACGTCATGGCAGTGAACGTTCCATCCTGGTTGGGGAACACGCCGca                       <  1:2137399/140‑1 (MQ=255)

                                                                                                                gcagGAGTAGACGTTTTAATCGTTAGCTTACTGTGTGCTTCTCCAACCATCGGCGCGCACCAGTTTCGGTTTTAAATGTTTTGCTTTTGGTATACGTCATGGCAGTGAACGTTCCATCCTGGTTGGGGAACACGCCGcac                      <  1:8338253/140‑1 (MQ=255)

                                                                                                                 cagGAGTAGACGTTTTAATCGTTAGCTTACTGTGTGCTTCTCCAACCATCGGCGCGCACCAGTTTCGGTTTTAAATGTTTTGCTTTTGGTATACGTCATGGCAGTGAACGTTCCATCCTGGTTGGGGAACACGCCGcaca                     >  1:848093/1‑140 (MQ=255)

                                                                                                                 cagGAGTAGACGTTTTAATCGTTAGCTTACTGTGTGCTTCTCCAACCATCGGCGCGCACCAGTTTCGGTTTTAAATGTTTTGCTTTTGGTATACGTCATGGCAGTGAACGTTCCATCCTGGTTGGGGAACACGCCGcaca                     >  2:5123616/1‑140 (MQ=255)

                                                                                                                   ggAGTAGACGTTTTAAGCGTTAGCTTAATGTGTGCTTCTCCAACCATCTGCGCGCACCAGTTTCGGTTTTAAATGTTTTGCTTTTGGTATACGTCATGGCAGTGAACGTTCCATCCTGGTTGGGGAACACGCCGCACAcc                   >  2:2046723/1‑140 (MQ=255)

                                                                                                                        aGACGTTTTAATCGTTAGCTTACTGTGTGCTTCTCCAACCATCGGCGCGCACCAGTTTCGGTTTTAAATGTTTTGCTTTTGGTATACGTCATGGCAGTGAACGTTCCATCCTGGTTGGGGAACACGCCGCACACCAGGGa              <  1:5834881/140‑1 (MQ=255)

                                                                                                                          aCGTTTTAATCGTTAGCTTACTGTGTGCTTCTCCAACCCTCGGCGCGCACCAGTTTCGGTTTTAAATGTTTTGCTTTTGGTATACGTCATGGCAGTGAACGTTCCATCCTGGTTGGGGAACACGCCGCACACCAGGGAtt            <  2:2205884/140‑1 (MQ=255)

                                                                                                                           cGTTTTAATCGTTAGCTTACTGTGTGCTTCTCCAACCATCGGCGCGCACCAGTTTCGGTTTTAAATGTTTTGCTTTTGGTCTACGTCATGGCAGTGAACGTTCCATCCTGGTTGGGGACCACGCCGCACACCAGGGATTc           >  2:4943680/1‑140 (MQ=255)

                                                                                                                              tttAATCGTTAGCTTACTGTGTGCTTCTCCAACCATCGGCGCGCACCAGTTTCGGTTTTAAATGTTTTGCTTTTGGTATACGTCATGGCAGTGAACGTTCCATCCTGGTTGGGGAACACGCCGCACACCAGGGATTCgtt        >  1:7048254/1‑140 (MQ=255)

                                                                                                                               ttAATCGTTAGCTTACTGTGTGCTTCTCCAACCATCGGCGCGCACCAGTTTCGGTTTTAAATGTTTTGCTTTTGGTATACGTCATGGCAGTGAACGTTCCATCCTGGTTGGGGAACACGCCGCACACCAGGGATTCgttg       >  1:5719479/1‑140 (MQ=255)

                                                                                                                               ttAATCGTTAGCTTACTGTGTGCTTCTCCAACCATCGGCGCGCACCAGTGTCGGTTTTAAATGTTTTGCTTTTGGTATACGTCATGGCAGTGAACGTTCCATCCTGGTTGGGGAACACGCCGCACACCAGGGATTCgttg       >  2:4582781/1‑140 (MQ=255)

                                                                                                                                    cGTTAGCTTACTGTGTGCTTCTCCAACCATCGGCGCGCACCAGTTTCGGTTTTAAATGTTTTGCTTTTGGTATACGTCATGGCAGTGAACGTTCCATCCTGGTTGGGGAACACGCCGCACACCAGGGATTCGTTGTTGcc  <  2:4210016/140‑1 (MQ=255)

ATGAAAAAATTAGAACGGAAGGGCATCATTCAACATCAGAGTGATAGCGCAAACTGTTCTTATTATCTCGTCACCGAAAAATACTCCCATTTTATGAAGTTATTCTGGAACAGCAGGAGTAGACGTTTTAATCGTTAACTTACTGTGTGCTTCTCCAACCATCGGCGCGCACCAGTTTCGGTTTTAAATGTTTTGCTTTTGGTATACGTCATGGCAGTGAACGTTCCATCCTGGTTGGGGAACACGCCGCACACCAGGGATTCGTTGTTGCC  >  NZ_CP025268/1423841‑1424112

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`