BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP025268	14,302	T→C	V45A (GTG→GCG)	dnaJ →	molecular chaperone DnaJ

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP025268	14,302	0	T	C	99.8%	1559.1 / NA	448	V45A (GTG→GCG)	dnaJ	molecular chaperone DnaJ
Reads supporting (aligned to +/- strand): ref base T (0/0); major base C (253/194); minor base A (0/1); total (253/195)
Fisher's exact test for biased strand distribution p-value = 4.35e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 448 total aligned reads displayed.

AAAGATGGCTAAGCAAGATTATTACGAGATTTTAGGCGTTTCCAAAACAGCGGAAGAGCGTGAAATCAGAAAGGCCTACAAACGCCTGGCCATGAAATACCACCCGGACCGTAACCAGGGTGACAAAGAGGCCGAGGTGAAATTTAAAGAGATCAAGGAAGCTTATGAAGTTCTGACCGACTCGCAAAAACGTGCGGCATACGATCAGTATGGTCATGCTGCGTTTGAGCAAGGTGGCATGGGCGGCGGCGGTTTTGGCGGCGGCGCAGACTTC  >  NZ_CP025268/14165‑14438

aaaGATGGCTAAGCAAGATTATTACGAGATTTTAGGCGTTTCCAAAACAGCGGAAGAGCGTGAAATCAGAAAGGCCTACAAACGCCTGGCCATGAAATACCACCCGGACCGTAACCAGGGTGACAAAGAGGCCGAGGCGa                                                                                                                                        <  2:2623826/140‑1 (MQ=255)

    aTGGCTAAGCAAGATTATTACGAGATTTTAGGCGTTTCCAAAACAGCGGAAGAGCGTGAAATCAGAAAGGCCTACAAACGCCTGGCCATGAAATACCACCCGGACCGTAACCAGGGTGACAAAGAGGCCGAGGCGAAAtt                                                                                                                                    >  1:1079816/1‑140 (MQ=255)

    aTGGCTAAGCAAGATTATTACGAGATTTTAGGCGTTTCCAAAACAGCGGAAGAGCGTGAAATCAGAAAGGCCTACAAACGCCTGGCCATGAAATACCACCCGGACCGTAACCAGGGTGACAAAGAGGCCGAGGCGAAAtt                                                                                                                                    >  2:5091942/1‑140 (MQ=255)

    aTGGCTAAGCAAGATTATTACGAGATTTTAGGCGTTTCCAAAACAGCGGAAGAGCGTGAAATCAGAAAGGCCTACAAACGCCTGGCCATGAAATACCACCCGGACCGTAACCAGGGTGACAAAGAGGCCGAGGCGAAAtt                                                                                                                                    >  1:3577700/1‑140 (MQ=255)

      ggCTAAGCAAGATTATTACGAGATTTTAGGCGTTTCCAAAACAGCGGAAGAGCGTGAAATCAGAAAGGCCTACAAACGCCTGGCCATGAAATACCACCCGGACCGTAACCAGGGTGACAAAGAGGCCGAGGCGAAATTTa                                                                                                                                  >  2:3919902/1‑140 (MQ=255)

        cTAAGCAAGATTATTACGAGATTTTAGGCGTTTCCAAAACAGCGGAAGAGCGTGAAATCAGAAAGGCCTACAAACGCCTGGCCATGAAATACCACCCGGACCGTAACCAGGGTGACAAAGAGGCCGAGGCGAAATTTaaa                                                                                                                                <  2:8410165/140‑1 (MQ=255)

        cTAAGCAACATTACTACGAGATTTTCCGCGTTTCCAAAACACCGGAAGAGCGTGAAATCAGAAAGTCCTACAAACGCCTGGCCATGAAATACCACCCGGACCGTAACCAGGGTGACAAAGAGGCCGAGGCGAAATTTaaa                                                                                                                                <  2:6656287/140‑1 (MQ=255)

           aGCAAGATTATTACGAGATTTTAGGCGTTTCCAAAACAGCGGAAGAGCGTGAAATCAGAAAGGCCTACAAACGCCTGGCCATGAAATACCACCCGGACCGTAACCAGGGTGACCAAGAGGCCGAGGCGAAATTGATagag                                                                                                                             >  1:3669806/1‑140 (MQ=255)

           aGCAAGATTATTACGAGATTTTAGGCGTTTCCAAAACAGCGGAAGAGCGTGAAATCAGAAAGGCCTACAAACGCCTGGCCATGAAATACCACCCGGACCGTAACCAGGGTGACAAAGAGGCCGAGGCGAAATTTAAagag                                                                                                                             <  1:7674447/140‑1 (MQ=255)

                 attattACGAGATTTTAGGCGTTTCCAAAACAGCGGAAGAGCGTGAAATCAGAAAGGCCTACAAACGCCTGGCCATGAAATACCACCCGGACCGTAACCAGGGTGACAAAGAGGCCGAGGCGAAATTTAAAGAGATCAAg                                                                                                                       <  2:1726948/140‑1 (MQ=255)

                 attattACGAGATTTTAGGCGTTTCCAAAACAGCGGAAGAGCGTGAAATCAGAAAGGCCTACAAACGCCTGGCCATGAAATACCACCCGGACCGTAACCAGGGTGACAAAGAGGCCGAGGCGAAATTTAAAGAGATCAAg                                                                                                                       >  1:7443035/1‑140 (MQ=255)

                 attattACGAGATTTTAGGCGTTTACAAAACAGCGGAAGAGCGTGAAATCAGAAAGGCCTACAAACGCCTGGCCATGAAATACCACCCGGACCGTAACCAGGGTGACAAAGAGGCCGAGGCGAAATTTAAAGAGATCAAg                                                                                                                       <  2:6398909/140‑1 (MQ=255)

                  ttattaCGAGATTTTAGGCGTTTCCAAAACAGCGGAAGAGCGTGAAATCAGAAAGGCCTACAAACGCCTGGCCATGAAATACCACCCGGACCGTAACCAGGGTGACCAAGAGGCCGAGGCGAAATTTAAAGAGATCAAgg                                                                                                                      >  1:6892748/1‑140 (MQ=255)

                   tattaCGAGATTTTAGGCTTTTCCAAAACAGCGGAAGAGCGTGAAATCAGAAAGGCCTACAAACGCCTGGCCATGAAATACCACCCGGACCGTAACCAGGGTGACAAAGAGGCCGAGGCGAAATTTAAAGAGATCAAGGa                                                                                                                     <  2:2955278/140‑1 (MQ=255)

                   tattaCGAGATTTTAGGCGTTTCCAAAACAGCGGAAGAGCGTGAAATCAGAAAGGCCTACAAACGCCTGGCCATGAAATACCACCCGGACCGTAACCAGGGTGACAAAGAGGCCGAGGCGAAATTTAAAGAGATCAAGGa                                                                                                                     <  2:4603356/140‑1 (MQ=255)

                     ttaCGAGATTTTAGGCGTTTCCAAAACAGCGGAAGAGCGTGAAATCAGAAAGGCCTACAAACGCCTGGCCATGAAATACCACCCGGACCGTAACCAGGGTGACAAAGAGGCCGAGGCGAAATTTAAAGAGATCAAGGAAg                                                                                                                   >  1:3388084/1‑140 (MQ=255)

                      taCGAGATTTTAGGCGTTTCCAAAACAGCGGAAGAGCGTGAAATCAGAAAGGCCTACAAACGCCTGGCCATGAAATACCACCCGGACCGTAACCAGGGTGACAAAGAGGCCGAGGCGAAATTTAAAGAGATCAAGGAAGc                                                                                                                  >  1:5621718/1‑140 (MQ=255)

                       aCGAGATTTTAGGCGTTTCCAAAACAGCGGAAGAGCGTGAAATCAGAAAGGCCTACAAACGCCTGGCCATGAAATACCACCCGGACCGTAACCAGGGTGACAAAGAGGCCGAGGCGAAATTTAAAGAGATCAAGGAAGCt                                                                                                                 >  2:5150050/1‑140 (MQ=255)

                           gaTTTTAGGCGTTTCCAAAACAGCGGAAGAGCGTGAAATCAGAAAGGCCTACAAACGCCTGGCCATGAAATACCACCCGGACCGTAACCAGGGTGACAAAGAGGCCGAGGCGAAATTTAAAGAGATCAAGGAAGCTTATg                                                                                                             >  1:6084813/1‑140 (MQ=255)

                           gaTTTTAGGCGTTTCCAAAACAGCGGAAGAGCGTGAAATCAGAAAGGCCTACAAACGCCTGGCCATGAAATACCACCCGGACCGTAACCAGGGTGACAAAGAGGCCGAGGCGAAATTTAAAGAGATCAAGGAAGCTTATg                                                                                                             <  1:4588079/140‑1 (MQ=255)

                           gaTTTTAGGCGTTTCCAAAACAGCGGAAGAGCGTGAAATCAGAAAGGCCTACAAACGCCTGGCCATGAAATACCACCCGGACCGTAACCAGGGTGACAAAGAGGCCGAGGCGAAATTTAAAGAGATCAAGGAAGCTTATg                                                                                                             <  1:5550461/140‑1 (MQ=255)

                                tAGGCGTTTCCAAAACAGCGGAAGAGCGTGAAATCAGAAAGGCCTACAAACGCCTGGCCATGAAATACCACCCGGACCGTAACCAGGGTGACAAAGAGGCCGAGGCGAAATTTAAAGAGATCAAGGAAGCTTATGAAGtt                                                                                                        >  2:1884921/1‑140 (MQ=255)

                                              aCAGCGGAAGAGCGTGAAATCAGAAAGGCCTACAAACGCCTGGCCATGAAAGACCACCCGGACCGTAACCAGGGTGACAAAGAGGCCGAGGCGAAAGTTAAAGAGATCAAGGAAGCTTATGAAGTTCTGACCGACTCGCa                                                                                          <  2:888611/140‑1 (MQ=255)

                                                 gCGGAAGAGCGTGAAATCAGAAAGGCCTACAAACGCCTGGCCATGAAATACCACCCGGACCGTAACCAGGGGGACAAAGAGGCCGAGGCGCAATTTATAGAGATCAAGGGAGCGTATGACGTGCTGAGCGACTCGCacaa                                                                                       >  1:7161490/1‑140 (MQ=255)

                                                  cGGAAGAGCGTGAAATCAGAAAGGCCTACAAACGCCTGGCCATGAAATACCACCCGGACCGTAACCAGGGTGACAAAGAGGCCGAGGCGAAATTTAAAGAGATCAAg                                                                                                                       >  2:2322678/1‑107 (MQ=255)

                                                  cGGAAGAGCGTGAAATCAGAAAGGCCTACAAACGCCTGGCCATGAAATACCACCCGGACCGTAACCAGGGTGACAAAGAGGCCGAGGCGAAATTTAAAGAGATCAAGGAAGCTTATGAAGTTCTGACCGACTCGCaaaat                                                                                      >  2:8750463/1‑139 (MQ=255)

                                                  cGGAAGAGCGTGAAATCAGAAAGGCCTACAAACGCCTGGCCATGAAATACCACCCGGACCGTAACCAGGGTGACAAAGAGGCCGAGGCGAAATTTAAAGAGATCAAGGAAGCTTATGAAGTTCTGACCGACTCGCaaaaa                                                                                      >  1:5027918/1‑140 (MQ=255)

                                                  cGGAAGAGCGTGAAATCAGAAAGGCCTACAAACGCCTGGCCATGAAATACCACCCGGACCGTAACCAGGGTGACAAAGAGGCCGAGGCGAAATTTAAAGAGATCAAGGAAGCTTATGAAGTTCTGACCGACTCGCaaaaa                                                                                      >  1:8177492/1‑140 (MQ=255)

                                                  cGGAAGAGCGTGAAATCAGAAAGGCCTACAAACGCCTGGCCATGAAATACCACCCGGACCGTAACCAGGGTGACAAAGAGGCCGAGGCGAAATTTAAAGAGATCAAGGAAGCTTATGAAGTTCTGACCGACTCGCaaaaa                                                                                      >  2:8265434/1‑140 (MQ=255)

                                                  cGGAAGAGCGTGAAATCAGAAAGGCCTACAAACGCCTGGCCATGAAATACCACCCGGACCGTAACCAGGGTGACAAAGAGGCCGAGGCGAAATTGAAAGAGATCAAGGAAGCTTATGAAGTTCTGACCGACTCGCaaaaa                                                                                      >  1:1792840/1‑140 (MQ=255)

                                                   ggAAGAGCGTGAAATCAGACAGGCCTACAAACGCCTGGCCATGAAATACCACCCGGACCGTAACCCGGGTGACAAAGAGGCCGAGGCGAAATTTAAATAGATCAAGGAAGCTTATGAAGTTATGACCGACTCGCAAAAAc                                                                                     >  1:412421/1‑140 (MQ=255)

                                                      agagCGTGAAATCAGAAAGGCCTACAAACGCCTGGCCATGAAATACCACCCGGACCGTAACCAGGGTGACAACGAGGCCGAGGCGAAATTTAAAGAGATCAAGGAAGCTTATGAAGTTCTGACCGACTCGCAAAAACGTg                                                                                  <  1:2329026/140‑1 (MQ=255)

                                                      agagCGTGAAATCAGAAAGGCCTACAAACGCCTGGCCATGAAATACCACCCGGACCGTAACCAGGGTGACAAAGAGGCCGAGGCGAAATTTAAAGAGATCAAGGAAGCTTATGAAGTTCTGACCGACTCGCAAAAACGTg                                                                                  <  1:1641281/140‑1 (MQ=255)

                                                      agagCGTGAAATCAGAAAGGCCTACAAACGCCTGGCCATGAAATACCACCCGGACCGTAACCAGGGTGACAAAGAGGCCGAGGCGAAATTTAAAGAGATCAAGGAAGCTTATGAAGTTCTGACCGACTCGCAAAAACGTg                                                                                  <  2:7444241/140‑1 (MQ=255)

                                                       gcgCGTCACATCAGAACGGCCTACCACCCCCTGCCCTAGCACGACCACCCGGCCCCGAACCAGGGTGACAACGCGCCCGCGGCGCAAATTAAAGAGATCACGGAAGCTTATGACGTTCTGACCGACTCGCAAAAACGTGc                                                                                 <  2:5391527/138‑1 (MQ=255)

                                                       gagCGTGAAATCAGACAGGCCTACAAACGCATGGCCATGAAATACCACCCGGACCGTAACCAGGGTGACAAAGAGGCCGAGGCGAAATTTAAAGAGATCAAGGAAGCTTATGAAGTTCTGACCGACTCGCAAAAACGTGc                                                                                 <  2:4148135/140‑1 (MQ=255)

                                                       gagCGTGAAATCAGAAAGGCCTACAAACGCCTGGCCATGAAATACCACCCGGACCGTAACCAGGGTGACAAAGAGGCCGAGGCGAAATTTAAAGAGATCAAGGAAGCTTATGAAGTTCTGACCGACTCGCAAAAACGTGc                                                                                 <  1:8521448/140‑1 (MQ=255)

                                                           gTGAAATCAGAAAGGCCTACAAACGCCTGGCCATGAAATACCACCCGGACCGTAACCAGGGTGACAAAGAGGCCGAGGCGAAATTTAAAGAGATCAAGGAAGCTTATGAAGTTCTGACCGACTCGCAAAAACGTGCGGCa                                                                             <  1:5846324/140‑1 (MQ=255)

                                                           gTGAAATCAGAAAGGCCTACAAACGCCTGGCCATGAAATACCACCCGGACCGTAACCAGGGTGACAAAGAGGCCGAGGCGAAATTTAAAGAGATCAAGGAAGCTTATGAAGTTCTGACCGACTCGCAAAAACGTGCGGCa                                                                             <  1:8717911/140‑1 (MQ=255)

                                                            tGAAATCAGAAAGGCCTACAAACGCCTGGCCATGAAATACCACCCGGACCGTAACCAGGGTGACAAAGAGGCCGAGGCGAAATTTAAAGAGATCAAGGAAGCTTATGAAGTTCTGACCGACTCGCAAAAACGTGCGGCAt                                                                            <  2:2106392/140‑1 (MQ=255)

                                                            tGAAATCAGAAAGGCCTACAAACGCCTGGCCATGAAATACCACCCGGACCGTAACCAGGGTGACAAAGAGGCCGAGGCGAAATTTAAAGAGATCAAGGAAGCTTATGAAGTTCTGACCGACTCGCAAAAACGTGCGGCAt                                                                            >  2:2012331/1‑140 (MQ=255)

                                                              aaaTCAGAAAGGCCTACAAACGCCTGGCCATGAAATACCACCCGGACCGTAACCAGGGTGACAAAGAGGCCGAGGCGAAATTTAAAGAGATCAAGGAAGCTTATGAAGTTCTGACCGACTCGCAAAAACGTGCGGCATAc                                                                          <  2:3971744/140‑1 (MQ=255)

                                                               aaTCAGAAAGGCCTACAAACGCCTGGCCATGAAATACCACCCGGACCGTTACCAGGGTGACTAAGAGGCCGAGGCGAAATTTAAAGAGATCAAGGAAGCTTTTGAAGTTCTGACCGACTCGCAAAAACGTGCGGCATACg                                                                         <  1:7348985/140‑1 (MQ=255)

                                                               aaTCAGAAAGGCCTACAAACGCCTGGCCATGAAATACCACCCGGACCGTAACCAGGGTGACAAAGAGGCCGAGGCGAAAATTAAAGAGATCAAGGAAGCTTATGAAGTTCTGACCGACTCGCAAAAACGTGCGGCATACg                                                                         <  1:5156315/140‑1 (MQ=255)

                                                               aaTCAGAAAGCCCTACAAACGCCTGGCCATGAAATACCACCCGGACCGTAACCAGGGTGACAAAGAGGCCGAGGCGAAATTTAAAGAGATCAAGGAAGATTATGAAGTTCTGACCGACTCGCAAAAACGTGCGGCATACg                                                                         <  1:1927492/140‑1 (MQ=255)

                                                                 tCAGAAAGGCCTACAAACGCCTGGCCATGAAATACCACCCGGACCGTAACCAGGGTGACAAAGAGGCCGAGGCGAAATTTAAAGAGATCAAGGAAGCTTATGAAGTTCTGACCGACTCGCAAAAACGTGCGGCATACGAt                                                                       <  1:3915486/140‑1 (MQ=255)

                                                                 tCAGAAAGGCCTACAAACGCCTGGCCATGAAATACCACCCGGACCGTAACCAGGGTGACAAAGAGGCCGAGGCGAAATTTAAAGAGATCAAGGAAGCTTATGAAGTTCTGACCGACTCGCAAAAACGTGCGGCATACGAt                                                                       <  2:3459964/140‑1 (MQ=255)

                                                                 tCAGAAAGGCCTACAAACGCCTGGCCATGAAATACCACCCGGACCGGAACCAGGGTGACAAAGAGGCCGAGGCGAAATTTAAAGAGATCAAGGAAGCTTATGAAGTTCTGACCGACTCGCAAAAACGTGCGGCATACGAt                                                                       <  2:430340/140‑1 (MQ=255)

                                                                      aaGGCCTACAAACGCCTGGCCATGAAATACCACCCGGACCGTAACCAGGGTGACAAAGCGGCCGAGGCGAAATTTAAAGAGATCAAGGAAGCTTATGAAGTTCTGACCGACTCGCAAAAACGTGCGGCATACGATCAGTa                                                                  <  1:3787785/140‑1 (MQ=255)

                                                                      aaGGCCTACAAACGCCTGGCCATGAAATACCACCCGGACCGTAACCAGGGTGACAAAGAGGCCGAGGCGAAATTTAAAGAGATCAAGGAAGCTTATGAAGTTCTGACCGACTCGCAAAAACGTGCGGCATACGATCAGTa                                                                  <  2:7298388/140‑1 (MQ=255)

                                                                         gCCTACAAACGCCTGGCCATGAAATACCACCCGGACCGTACCCAGGGTGACCAAGAGGCCGGGGCGAAATTTAAAGAGATCACGGAAGCTTATGAAGGTCTGACCGACTCGCAAAAACGTGCGGCATACGATCAGTATgg                                                               >  2:1516611/1‑140 (MQ=255)

                                                                          ccTACAAACGCCTGGCCATGAAATACCACCCGGACCGTAACCAGGGTGACAAAGAGGCCGAGGCGAAATTTAAAGAGATCAAGGAAGCTTATGAAGTTCTGACCGACTCGCAAAAACGTGCGGCATACGATCAGTATGGt                                                              <  2:4930570/140‑1 (MQ=255)

                                                                             aCAAACGCCTGGCCATGAAATACCACCCGGACCGTAACCAGGGTGACAAAGAGGCCGAGGCGAAATTTAAAGAGATCAAGGAAGCTTATGAAGTTCTGACCGACTCGCAAAATCGTGCGGCATACGATCAGTATGGTCAt                                                           >  1:4861144/1‑140 (MQ=255)

                                                                             aCAAACGCCTGGCCATGAAATACCACCCGGACCGTAACCAGGGTGACAAAGAGGCCGAGGCGAAATTTAAAGAGATCAAGGAAGCTTATGAAGTTCTGACCGACTCGCAAAAACGTGCGGCATACGATCAGTATGGTCAt                                                           >  1:3439518/1‑140 (MQ=255)

                                                                               aaaCGCCTGGCCATGAAATACCACCCGGACCGTAACCAGGGTGACAAAGAGGCCGAGGCGAAATTTAAAGAGATCACGGAAGCTTATGAAGTTCTGACCGACTCGCAAAAACGTGCGGCATACGATCAGTATGGTCAtgc                                                         <  2:470348/140‑1 (MQ=255)

                                                                                   gCCTGGCCATGAAATACCACCCGGACCGTAACCAGGGTGACAAAGAGGCCGAGGCGAAATTTAAAGTGTTCAAGGAAGCGTATGAAGTTCTGACCGACTCGCAAAAACGTGCGGCATACGATCAGTATGGTCATGCTGCg                                                     >  2:9335102/1‑140 (MQ=255)

                                                                                   gCCTGGCCATGAAATACCACCCGGACCGTAACCAGGGTGACAAAGAGGCCGAGGCGAAATTTAAAGAGATCAAGGAAGCTTATGAAGTTCTGACCGACTCGCAAAAACGTGc                                                                                 >  1:2068940/1‑112 (MQ=255)

                                                                                    ccTGGCCATGAAATACCACCCGGACCGTAACCAGGGTGACAAAGAGGCCGAGGCGAAATTTAAAGAGATCAAGGAAGCTTATGAATTTCTGACCGACTCGCAAAAACGTGCGGCATACGATCAGTATGGTCATGCTGCGt                                                    <  1:5352082/140‑1 (MQ=255)

                                                                                     cTGGCCATGAAATACCACCCGGACCGTAACCAGGGTGACAAAGAGGCCGAGGCGAAATTTAAAGAGATCAAGGAAGCTTATGAAGTTCTGACCGACTCGCAAAAACGTGCGGCATACGATCAGTATGGTCATGCTGCGtt                                                   >  1:5655386/1‑140 (MQ=255)

                                                                                      tGGCCATGAAATACCACCCGGACCGTAACCAGGGTGACAAAGAGGCCGAGGCGAAATTTAAAGAGATCATGGAAGCTGATGAAGTTCTGACCGACTCGCAAAAACGTGCGGCATCCGATCAGTATGGTCCTGCTGCGttt                                                  >  2:2847303/1‑140 (MQ=255)

                                                                                      tGGCCATGAAATACCACCCGGACCGTAACCAGGGTGACAAAGAGGCCGAGGCGAAATTTAAAGAGATCAAGGAAGCTTATGAAGTTCTGACCGACTCGCAAAAACGTGCGGCATACGATCAGTATGGTCATGCTGCGttt                                                  <  2:1175821/140‑1 (MQ=255)

                                                                                           aTGAAATACCCCCCGGACCGGAACCAGGGTGACAAAGAGGCCGAGGCGACATTTAACGAGATCACGGAAGCTTATGAAGTTCTGACCGACTCGCAAAAACGTGCGGCATACGATCAGTATGGTCATGCTGCGTTTGAGCa                                             <  1:2912999/140‑1 (MQ=255)

                                                                                             gAAATACCCCCCGGACCGGAACCAGGGTGACACAGCGGCCGAGGCGAAATTTCAAGAGATCAAGGACGCTTATGAAGTTCTGACCGACTCGCAAAAACGTGCGGCATACGATCAGTATGGTCATGCTGCGTTTGAGCAAg                                           <  1:7960001/140‑1 (MQ=255)

                                                                                             gAAATACCACCCGGACCGTAACCAGGGTGACAAAGAGGCCGAGGCGAAATTTAAAGAGATCAAGGAAGCTTATGAAGTTCTGACCGACTCGCAAAAACGTGCGGCATACGATCAGTATGGTCATGCTGCGTTTGAGCAAg                                           >  1:9246920/1‑140 (MQ=255)

                                                                                             gAAATACCACCCGGACCGTAACCAGGGTGACAAAGAGGCCGAGGCGAAATTTAAAGAGATCAAGGAAGCTTATGAAGTTCTGACCGACTCGCAAAAACGTGCGGCATACGATCAGTATGGTCATGCTGCGTTTGAGCAAg                                           >  1:7842177/1‑140 (MQ=255)

                                                                                             gAAATACCACCCGGACCGTAACCAGGGTGACAAAGAGGCCGAGGCGAAATTTAAAGAGATCAAGGAAGCTTATGAAGTTCTGACCGACTCGCAAAAACGTGCGGCATACGATCAGTATGGTCATGCTGCGTTTGAGCAAg                                           >  2:8949239/1‑140 (MQ=255)

                                                                                                  accaccCGGACCGTAACCAGGGTGACAAAGAGGCCGAGGCGAAATTTAAAGAGATCAAGGAAGCTTATGAAGTTCTGACCGACTCGCAAAAACGTGCGGCATACGATCAGTATGGTCATGCTGCGTTTGAGCAAGGTGGc                                      <  1:8417956/140‑1 (MQ=255)

                                                                                                      cccGGACCGTAACCAGGGTGACAAAGAGGCCGAGGCGAAATTTAAAGAGATCAAGGAAGCTTATGAAGTTCTGACCGACTCGCAAAAACGTGCGGCATACGATCAGTATGGTCATGCTGCGTTTGAGCAAGGTGGCATgg                                  >  2:9261459/1‑140 (MQ=255)

                                                                                                      cccGGACCGTAACCAGGGTGACAAAGAGGCCGAGGCGAAATTTAAAGAGATCAAGGAAGCTTATGAAGTTCTGACCGACTCGCAAAAACGTGCGGCATACGATCAGTATGGTCATGCTGCGTTTGAGCAAGGTGGCATgg                                  >  2:1325775/1‑140 (MQ=255)

                                                                                                      cccGGACCGTAACCAGGGTGACAAAGAGGCCGAGGCGAAATTTAAAGAGATCAAGGAAGCTTATGAAGTTCTGACCGACTCGCAAAAACGTGCGGCATACGATCAGTATGGTCATGCTGCGTTTGAGCAAGGTGGCATgg                                  >  1:1272668/1‑140 (MQ=255)

                                                                                                        cGGACCGTAACCAGGGTGACAAAGAGGCCGAGGCGAAATTTAAAGAGATCAAGGAAGCTTATGAAGTTCTGACCGACTCGCAAAAACGTGCGGCATACGATCAGTCTGGTCATGCTGCGTTTGAGCACGGTGGCATGggc                                >  2:7030154/1‑140 (MQ=255)

                                                                                                         ggACCGTAACCAGGGTGACAAAGAGGCCGAGGCGAAATTTAAAGAGATCAAGGAAGCTTATGAAGTTCTGACCGACTCGCAAAAACGTGCGGCATACGATCAGTATGGTCATGCTGCGTTTGAGCAAGGTGGCATGggcg                               >  2:3743466/1‑140 (MQ=255)

                                                                                                         ggACCGTAACCAGGGTGACAAAGAGGCCGAGGCGAAATTTAAAGAGATCAAGGAAGCTTATGAAGTTCTGACCGACTCGCAAAAACGTGCGGCATACGATCAGTATGGTCATGCTGCGTTTGAGCAAGGTGGCATGggcg                               >  1:9466804/1‑140 (MQ=255)

                                                                                                          gACCGTAACCAGGGTGACAAAGAGGCCGAGGCGAAATTTAAAGAGATCAAGGAAGCTTATGAAGTTCTGACCGACTCGCAAAAACGTGCGGCATACGATCAGTATGGTCATGCTGCGTTTGAGCAAGGTGGCATGggcgg                              >  1:3199420/1‑140 (MQ=255)

                                                                                                             cGTAACCAGGGTGACAAAGAGGCCGAGGCGAAATTTAAAGAGATCAAGGAAGCTTATGAAGTTCTGACCGACTCGCAAAAACGTGCGGCATACGATCAGTATGGTCATGCTGCGTTTGAGCAAGGTGGCATGggcggcgg                           >  2:4487366/1‑140 (MQ=255)

                                                                                                              gTAACCAGGGTGACAAAGAGGCCGAGGCGAAATTTAAAGAGATCAAGGAAGCTTATGAAGTTCTGACCGACTCGCAAAAACGTGCGGCATACGATCAGTATGGTCATGCTGCGTTTGAGCAAGGTGGCATGggcggcggc                          >  2:718488/1‑140 (MQ=255)

                                                                                                              gTAACCAGGGTGACAAAGAGGCCGAGGCGAAATTTAAAGAGATCAAGGAAGCTTATGAAGTTCTGACCGACTCGCAAAAACGTGCGGCATACGATCAGTATGGTCATGCTGCGTTTGAGCAAGGTGGCATGggcggcggc                          >  1:2580155/1‑140 (MQ=255)

                                                                                                              gTAACCAGGGTGACAAAGAGGCCGAGGCGAAATTTAAAGAGATCAAGGAAGCTTATGAAGTTCTGACCGACTCGCAAAAACGTGCGGCATACGATCAGTATGGTCATGCTGCGTTTGAGCAAGGTGGCATGggcggcggc                          >  1:6538406/1‑140 (MQ=255)

                                                                                                                    aGGGTGACAAAGAGGCCGAGGCGAAATTTAAAGAGATCAAGGAAGCTTATGAAGTTCTGACCGACTCGCAAAAACGTGCGGCATACGATCAGTATGGTCATGCTGCGTTTGAGCAAGGTGGCATGGGCGGCGGCGGtttt                    <  1:4249368/140‑1 (MQ=255)

                                                                                                                     gggTGACAAAGAGGCCGAGGCGAAATTTAAAGAGATCAAGGAAGCTTATGAAGTTCTGACCGACTCGCAAAATCGTGCGGCATACGATGAGTTTGGTCATGCTGCGTGTGAGGAAGGTGGCATGGGCGGCGGCGGTtgtg                   >  2:7732153/1‑140 (MQ=255)

                                                                                                                     gggTGACAAAGAGGCCGAGGCGAAATTTAAAGAGATCAAGGAAGCTTATGAAGTTCTGACCGACTCGCAAAAACGTGCGGCATACGATCAGTATGGTCATGCTGCGTTTGAGCAAGGTGGCATGGGCGGCGGCGGTTTTg                   >  1:8569373/1‑140 (MQ=255)

                                                                                                                     gggTGACAAAGAGGCCGAGGCGAAATTTAAAGAGATCAAGGAAGCTTATGAAGTTCTGACCGACTCGCAAAAACGTGCGGCATACGATCAGTATGGTCATGCTGCGTTTGAGCAAGGTGGCATGGGCGGCGGCGGTTTTg                   >  1:308502/1‑140 (MQ=255)

                                                                                                                     gggTGACAAAGAGGCCGAGGCGAAATTTAAAGAGATCAAGGAAGCTTATGAAGTTCTGACCGACTCGCAAAAACGTGCGGCATACGATCAGTATGGTCATGCTGCGTTTGAGCAAGGTGGCATGGGCGGCGGCGGTTTTg                   >  1:7254591/1‑140 (MQ=255)

                                                                                                                      ggTGACAAAGAGGCCGAGGCGAAATTTAAAGAGATCAAGGAAGCTTATGAAGTTCTGACCGACTCGCAAAAACGTGCGGCATACGATCAGTATGGTCATGCTGCGTTTGAGCAAGGTGGCATGGGCGGCGGCGGTTTTgg                  >  1:6403665/1‑140 (MQ=255)

                                                                                                                         gACAAAGAGGCCGAGGCGAAATTTAAAGAGATCATGGAAGCTTATGAAGTTCTGACCGACTCGCAAAAACGTGCGGCATACGATCAGTATGGTCATGCTGCGTTTGAGCAAGGTGGCATGGGCGGCGGCGGTTTTggcgg               >  2:6792586/1‑140 (MQ=255)

                                                                                                                          aCAAAGAGGCCGAGGCGAAATTTAAAGAGATCAAGGAAGCTTATGAAGTTCTGACCGACTCGCAAAAACGTGCGGCATACGAt                                                                       <  2:6208132/83‑1 (MQ=255)

                                                                                                                          aCAAAGAGGCCGAGGCGAAATTTAAAGAGATCAAGGAAGCTTATGAAGTTCTGACCGACTCGCAAAAACGTGCGGCATACGAt                                                                       >  1:6208132/1‑83 (MQ=255)

                                                                                                                          aCAAAGAGGCCGAGGCGAAATTTAAAGAGATCAAGGAAGCTTATGAAGTTCTGACCGACTCGCAAAAACGTGCGGCATACGATCAGTCTGGTCATGCTGCGTTTGAGCAAGGTGGCATGGGCGGCGGCGGTTTTggcggc              >  2:6062427/1‑140 (MQ=255)

                                                                                                                          aCAAAGAGGCCGAGGCGAAATTTAAAGAGATCAAGGAAGCTTATGAAGTTCTGACCGACTCGCAAAAACGTGCGGCATACGATCAGTATGGTCATGCTGCGTTTGAGCAAGGTGGCATGGGCGGCGGCGGTTTTggcggc              >  2:2511755/1‑140 (MQ=255)

                                                                                                                          aCAAAGAGGCCGAGGCGAAATTTAAAGAGATCAAGGAAGCTTATGAAGTTCTGACCGACTCGCAAAAACGTGCGGCATACGATCAGTATGGTCATGCTGCGTTTGAGCAAGGTGGCATGGGCGGCGGCGGTTTTggcggc              >  2:5935415/1‑140 (MQ=255)

                                                                                                                          aCAAAGAGGCCGAGGCGAAATTTAAAGAGATCAAGGAAGCTTATGAAGTTCTGACCGACTCGCAAAAACGTGCGGCATACGATCAGTATGGTCATGCTGCGTTTGAGCAAGGTGGCATGGGCGGCGGCGGTTTTggcggc              >  1:958740/1‑140 (MQ=255)

                                                                                                                              agagGCCGAGGCGAAATTTAAAGAGATCAAGGAAGCTTATGAAGTTCTGACCGACTCGCAAAAACGTGCGGCATACGATCAGTATGGTCATGCTGCGTTTGAGCAAGGTGGCATGGGCGGCGGCGGTTTTGgcggcggcg          <  2:8031921/140‑1 (MQ=255)

                                                                                                                              agagGCCGAGGCGAAATTTAAAGAGATCAAGGAAGCTTATGAAGTTCTGACCGACTCGCAAAAACGTGCGGCATACGATCAGTATGGTCATGCTGCGTTTGAGCAAGGTGGCATGGGCGGCGGCGGTTTTGgcggcggcg          >  1:5718775/1‑140 (MQ=255)

                                                                                                                                agGCCGAGGCGAAATTTAAACAGATCAAGGAAGCTTATGAATTTCTGGCCGACTCGCAAAAACGTGCGGCATACGATCAGTATGGTCATGCTGCGTTTGAGCAAGGTGGCATTGGCGGCGGCGGTTTTGTCGGCGGCGCa        <  1:472013/140‑1 (MQ=255)

                                                                                                                                  gCCGAGGCGAAATTTAAAGAGATCAAGGAAGCTTATGAAGTTCTGACCGACTCGCAAAAACGTGCGGCATACGATCAGTATGGTCATGCTGCGTTTGCGCAAGGTGGCATGGGCGGCGGCGGTTTTGGCGGCGGCGCAGa      >  2:5754702/1‑140 (MQ=255)

                                                                                                                                    cGAGGCGAGATTGAAAGAGATCACGGAAGCTTATGAAGTTCTGACCGACTCGCAAAATCGTGCGGCATACGATCAGTATGGTCATGCTGCGTTTGTGCAAGGTGGCATGGGCGGCGGCGGTTTTGGCGGCGGCGCAGagg    >  2:3039331/1‑138 (MQ=255)

                                                                                                                                    cGAGGCGAAATTTAAAGAGATCAAGGAAGCTTATGAAGTTCTGACCGACTCGCAAAAACGTGCGGCATACGATCAGTATGGTCATGCTGCGTTTGAGCAAGGTGGCATGGGCGGCGGCGGTTTTGGCGGCGGCGCAGACt    >  1:2715429/1‑140 (MQ=255)

                                                                                                                                    cGAGGCGAAATTTAAAGAGATCAAGGAAGCTTATGAAGTTCTGACCGACTCGCAAAAACGTGCGGCATACGATCAGTATGGTCATGCTGCGTTTGAGCAAGGTGGCATGGGCGGCGGCGGTTTTGGCGGCGGCGCAGACt    >  1:1042871/1‑140 (MQ=255)

                                                                                                                                      aGGCGAAATTTAAAGAGATCAAGGAAGCTTATGAAGTTCTGACCGACTCGCAAAAACGTGCGGCATACGATCAGTATGGTCATGCTGCGTTTGAGCAAGGTGGCATGGGCGGCGGCGGTTTTGGCGGCGGCGCAGACTTc  >  2:8568463/1‑140 (MQ=255)

                                                                                                                                      aGGCGAAATTTAAAGAGATCAAGGAAGCTTATGAAGTTCTGACCGACTCGCAAAAACGTGCGGCATACGATCAGTATGGTCATGCTGCGTTTGAGCAAGGTGGCATGGGCGGCGGCGGTTTTGGCGGCGGCGCAGACTTc  >  2:7866409/1‑140 (MQ=255)

AAAGATGGCTAAGCAAGATTATTACGAGATTTTAGGCGTTTCCAAAACAGCGGAAGAGCGTGAAATCAGAAAGGCCTACAAACGCCTGGCCATGAAATACCACCCGGACCGTAACCAGGGTGACAAAGAGGCCGAGGTGAAATTTAAAGAGATCAAGGAAGCTTATGAAGTTCTGACCGACTCGCAAAAACGTGCGGCATACGATCAGTATGGTCATGCTGCGTTTGAGCAAGGTGGCATGGGCGGCGGCGGTTTTGGCGGCGGCGCAGACTTC  >  NZ_CP025268/14165‑14438

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`