Predicted mutation
evidence seq id position mutation annotation gene description
RA NZ_CP025268 1,879,612 A→G *149* (TAA→TAG) 
pseudogene (778/821 nt)		 yeaL →
nimR ←		 DUF441 domain‑containing protein
DNA‑binding transcriptional regulator NimR

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0252681,879,6120AG100.0% 1916.3 / NA 547*149* (TAA→TAG) 
pseudogene (778/821 nt)		yeaL
nimR		DUF441 domain‑containing protein
DNA‑binding transcriptional regulator NimR
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (178/369);  total (178/369)

Only 100 of 558 total aligned reads displayed.

ACGTTAATGGGCAGCCAGCCGCAACTGGTCGCCGGGCTGCTGGTAGGCACTGTTTTAGGCGTAGCGCTGTTTCGCGGCGTACCGGTTGGGCCGCTTATTGCCGCGGGTCTGGTTTCGCTGATTGTGGGGAAACAGTAATTAATCTCGCAATATATCGGCCTGGCGTTTGCCCCAGGCCTTTCTTAAACATAGTAATAAAGGCCGTTGTTGAATCATACCCCAGCGTATGAGCAACTTTCTGTACAGTATCGCCTTTCACTAATCCCTGAAGCGC  >  NZ_CP025268/1879475‑1879748
                                                                                                                                         |                                                                                                                                        
aCGTTAATGGGCAGCCAGCCGCAACTGGTCGCCGGGCTGCTGGTAGGCACTGTTTTAGGCGTAGCGCTGTTTCGCGGCGTACCGGTTGGGCCGCTTATTGCCGCGGGTCTGGTTTCGCTGATTGTGGGGAAACAGtagtt                                                                                                                                        <  2:9258946/140‑1 (MQ=255)
     aaTGGGCAGCCAGCCGCAACTGGTCGCCGGGCTGCTGGTAGGCACTGTTTTAGGCGTAGCGCTGTTTCGCGGCGTACCGGTTGGGCCGCTTATTGCCGCGGGTCTGGTTTCGCTGATTGTGGGGAAACAGTAGTTAAtct                                                                                                                                   <  1:101326/140‑1 (MQ=255)
     aaTGGGCAGCCAGCCGCAACTGGTCGCCGGGCTGCTGGTAGGCACTGTTTTAGGCGTAGCGCTGTTTCGCGGCGTACCGGTTGGGCCGCTTATTGCCGCGGGTCTGGTTTCGCTGATTGTGGGGAAACAGTAGTTAAtct                                                                                                                                   <  2:2431345/140‑1 (MQ=255)
     aaTGGGCAGCCAGCCGCAACTGGTCGCCGGGCTGCTGGTAGGCACTGTTTTAGGCGTAGCGCTGTTTCGCGGCGTACCGGTTGGGCCGCTTATTGCCGCGGGTCTGGTTTCGCTGATTGTGGGGAAACAGTAGTTAAtct                                                                                                                                   <  2:3364040/140‑1 (MQ=255)
       tGGGCAGCCAGCCGCAACTGGTCGCCGGGCTGCTGGTAGGCACTGTTTTAGGCGTAGCGCTGTTTCGCGGCGTACCGGTTGGGCCGCTTATTGCCGCGGGTCTGGTTTCGCTGATTGTGGGGAAACAGTAGTTAATCTCg                                                                                                                                 <  1:897500/140‑1 (MQ=255)
         ggCAGCCAGCCGCAACTGGTCGCCGGGCTGCTGGTAGGCACTGTTTTTGGCGTAGCGCTGTTTCGCGGCGTACCGGTTGGGCCGCTTATTGCCGCGGGTCTGGTTTCGCTGATTGTGGGGAAACAGTAGTTAATCTCGCa                                                                                                                               <  2:7865190/140‑1 (MQ=255)
         ggCAGCCAGCCGCAACTGGTCGCCGGGCTGCTGGTAGGCACTGTTTTAGGCGTAGCGCTGTTTCGCGGCGTACCGGTTGGGCCGCTTATTGCCGCGGGTCTGGTTTCGCTGATTGTGGGGAAACAGTAGTTCATCTCGCa                                                                                                                               <  2:9000451/140‑1 (MQ=255)
         ggCAGCCAGCCGCAACTGGTCGCCGGGCTGCTGGTAGGCACTGTTTTAGGCGTAGCGCTGTTTCGCGGCGTACCGGTTGGGCCGCTTATTGCCGCGGGTCTGGTTTCGCTGATTGTGGGGAAACAGTAGTTAATCTCGCa                                                                                                                               <  1:7353088/140‑1 (MQ=255)
         ggCAGCCAGCCGCAACTGGTCGCCGGGCTGCTGGTAGGCACTGTTTTAGGCGTAGCGCTGTTTCGCGGCGTACCGGTTGGGCCGCTTATTGCCGCGGGTCTGGTTTCGCTGATTGTGGGGAAACAGTAGTTAATCTCGCa                                                                                                                               <  2:5717238/140‑1 (MQ=255)
         ggCAGCCAGCCGCAACTGGTCGCCGGGCTGCTGGTAGGCACTGTTTTAGGCGTAGCGCTGTTTCGCGGCGTACCGGTTGGGCCGCTTATTGCCGCGGGTCTGGTTTCGCTGATTGTGGGGAAACAGTAGTTAATCTCGCa                                                                                                                               <  2:3237428/140‑1 (MQ=255)
           cagccagcCGCAACTGGTCGCCGGGCTGCTGGTAGGCACTGTTTTAGGCGTAGCGCTGTTTCGCGGCGTACCGGTTGGGCCGCTTATTGCCGCGGGTCTGGTTTCGCTGATTGTGGGGAAACAGTAGTTAATCTCGCAat                                                                                                                             >  2:2619431/1‑140 (MQ=255)
             gccagccGCAACTGGTCGCCGGGCTGCTGGTAGGCACTGTTTTAGGCGTAGCGCTGTTTCGCGGCGTACCGGTTGGGCCGCTTATTGCCGCGGGTCTGGTTTCGCTGATTGTGGGGAAACAGTAGTTAATCTCGCAatat                                                                                                                           >  2:226511/1‑140 (MQ=255)
              ccagccGCAACTGGTCGCCGGGCTGCTGGTAGGCACTGTTTTAGGCGTAGCGCTGTTTCGCGGCGTACCGGTTGGGCCGCTTATTGCCGCGGGTCTGGTTTCGCTGATTGTGGGGAAACAGTAGTTAATCTCGCAatata                                                                                                                          >  1:9019657/1‑140 (MQ=255)
              ccagccGCAACTGGTCGCCGGGCTGCTGGTAGGCACTGTTTTAGGCGTAGCGCTGTTTCGCGGCGTACCGGTTGGGCCGCTTATTGCCGCGGGTCTGGTTTCGCTGATTGTGGGGAAACAGTAGTTAATCTCGCAatata                                                                                                                          <  2:7378420/140‑1 (MQ=255)
              ccagccGCAACTGGTCGCCGGGCTGCTGGTAGGCACTGTATTAGGCGTAGCGCTGTTTCGCGGCGTACCGGTTGGGCCGCTTATTGCCGCGGGTCTGGTTTCGCTGATTGTGGGGAAACAGTAGTTAATCTCGCAatata                                                                                                                          <  2:3647531/140‑1 (MQ=255)
              ccagccGCAACTGGTCGCCGGGCTGCTGGGAGGCACTGTTTTAGGCGTAGCGCTGTTTCGCGGCGTACCGGTTGGGCCGCTTATTGCCGCGGGTCTGGTTTCGCTGATTGTGGGGAAACAGTAGTTAATCTCGCAatata                                                                                                                          <  2:4166722/140‑1 (MQ=255)
                           gTCGCCGGGCTGCTGGTAGGCACTGTTTTAGGCGTAGCGCTGTTTCGCGGCGTACCGGTTGGGCCGCTTATTGCCGCGGGTCTGGTTTCTCTGATTGTGGGGAAACAGTAGTTAATCTCGCAATATATCGGCCTGGCGtt                                                                                                             >  2:8934560/1‑140 (MQ=255)
                           gTCGCCGGGCTGCTGGTAGGCACTGTTTTAGGCGTAGCGCTGTTTCGCGGCGTACCGGTTGGGCCGCTTATTGCCGCGGGTCTGGTTTCGCTGATTGTGGGGAAACAGTAGTTAATCTCGCAATATATCGGCCTGGCGtt                                                                                                             >  2:473025/1‑140 (MQ=255)
                           gTCGCCGGGCTGCTGGTAGGCACTGTTTTAGGCGTAGCGCTGTTTCGCGGCGTACCGGTTGGGCCGCTTATTGCCGCGGGTCTGGTTTCGCTGATTGTGGGGAAACAGTAGTTAATCTCGCAATATATCGGCCTGGCGtt                                                                                                             <  1:4979873/140‑1 (MQ=255)
                           gTCGCCGGGCTGCTGGTAGGCACTGTTTTAGGCGTAGCGCTGTTTCGCGGCGTACCGGTTGGGCCGCTTATTGCCGCGGGTCTGGTTTCGCTGATTGTGGGGAAACAGTAGTTAATCTCGCAATATATCGGCCTGGCGtt                                                                                                             <  1:1421303/140‑1 (MQ=255)
                           gTCGCCGGGCTGCTGGTAGGCACTGTTTTAGGCGTAGCGCTGTTTCGCGGCGTACCGGTTGGGCCGCTTATTGCCGCGGGTCTGGTTTCGCTGATTGTGGGGAAACAGTAGTTAATCTCGCAATATATCGGCCTGGCGtt                                                                                                             >  1:2637558/1‑140 (MQ=255)
                                cGGGCTGCTGGTAGGCACTGTTTTAGGCGTAGCGCTGTTTCGCGGCGTACCGGTTGGGCCGCTTATTGCCGCGGGTCTGGTTTCGCTGATTGTGGGGAAACAGTAGTTAATCTCGCAATATATCGGCCTGGCGTTTGccc                                                                                                        <  1:3703475/140‑1 (MQ=255)
                                     tgctgGTAGGCACTGTTTTAGGCGTAGCGCTGTTTCGCGGCGTACCGGTTGGGCCGCTTATTGCCGCGGGTCTGGTTTCGCTGATTGTGGGGAAACAGTAGTTAATCTCGCAATATATCGGCCTGGCGTTTGCCCCAGGc                                                                                                   <  2:1045898/140‑1 (MQ=255)
                                     tgctgGTAGGCACTGTTTTAGGCGTAGCGCTGTTTCGCGGCGTACCGGTTGGGCCGCTTATTGCCGCGGGTCTGGTTTCGCTGATTGTGGGGAAACAGTAGTTAATCTCGCAATATATCGGCCTGGCGTTTGCCCCAGGc                                                                                                   <  1:1569593/140‑1 (MQ=255)
                                     tgctgGTAGGCACTGTTTTAGGCGTAGCGCTGTTTCGCGGCGTACCGGTTGGGCCGCTTATTGCCGCGGGTCTGGTTTCGCTGATTGTGGGGAAACAGTAGTTAATCTCGCAATATATCGGCCTGGCGTTTGCCCCAGGc                                                                                                   <  2:8515766/140‑1 (MQ=255)
                                     tgctgGTAGGCACTGTTTTAGGCGTAGCGCTGTTTCGCGGCGTACCGGTTGGGCCGCTTATTGCCGCGGGTCTGGTTTCGCTGATTGTGGGGAAACAGTAGTTAATCTCGCAATATATCGGCCTGGCGTTTGCCCCAGGc                                                                                                   <  1:6926665/140‑1 (MQ=255)
                                     tgctgGTAGGCACTGTTTTAGGCGTAGCGCTGTTTCGCGGCGTACCGGTTGGGCCGCTTATTGCCGCGGGTCTGGTTTCGCTGATTGTGGGGAAACAGTAGTTAATCTCGCAATATATCGGCCTGGCGTTTGCCCCAGGc                                                                                                   <  1:5800472/140‑1 (MQ=255)
                                     tgctgGTAGGCACTGTTTTAGGCGTAGCGCTGTTTCGCGGCGTACCGGTTGGGCCGCTTATTGCCGCGGGTCTGGTTTCGCTGATTGTGGGGAAACAGTAGTTAATCTCGCAATATATCGGCCTGGCGTTTGCCCCAGGc                                                                                                   <  1:3464779/140‑1 (MQ=255)
                                     tgctgGTAGGCACTGTTTGAGGCGTAGCGCTGTTTCGCGGCGTACCGGTTGTGCCGCTTATTGCCGCGGGTCTGGTTTCGCTGATTGTGGGGAAACAGTAGTTAATCTCGCAATATATCGGCCTGGCGTTTGCCCCAGGc                                                                                                   <  2:3870879/140‑1 (MQ=255)
                                     tgctgGTAGGCACTGTATTAGGCGTAGCGCTGGTGCGCGGCGTACCGGTTGGGCCGCTTATTGCCGCGGGTCTGGTGTCGCTGATTGTGGGGAAACAGTAGTTAATCTCGCAATATATCGGCCTGGCGTTTGCCCCAGGc                                                                                                   <  2:8216723/140‑1 (MQ=255)
                                     tgctgGTAGGCACAGTTTTAGGCGTAGCGCTGCTTCGCGGCGTACCGGTTGGGCCGCTTATTGCCGCGGGTCTGGTTTCGCTGATTGTGGGGAAACAGTAGTTAATCTCGCAATATATCGGCCTGGCGTTTGCCCCAGGc                                                                                                   <  1:6223620/140‑1 (MQ=255)
                                       ctgGTAGGCACTGTTTTAGGCGTAGCGCTGTTTCGCGGCGTACCGGTTGGGCCGCTTATTGCCGCGGGTCTGGTTTCGCTGATTGTGGGGAAACAGTAGTTAATCTCGCAATATATCGGCCTGGCGTTTGCCCCAGGCCt                                                                                                 <  1:3521879/140‑1 (MQ=255)
                                       ctgGTAGGCACTGTTTTAGGCGTAGCGCTGTTTCGCGGCGTACCGGTTGGGCCGCTTATTGCCGCGGGTCTGGTTTCGCTGATTGTGGGGAAACAGTAGTTAATCTCGCAATATATCGGCCTGGCGTTTGCCCCAGGCCt                                                                                                 <  1:8283299/140‑1 (MQ=255)
                                         ggTAGGCACTGTTTTAGGCGTAGCGCTGTTTCGCGGCGTACCGGTTGGGCCGCTTATTGCCGCGGGTCTGGTTTCGCTGATTGTGGGGAAACAGTAGTTAATCTCGCAATATATCGGCCTGGCGTTTGCCCCAGGCCttt                                                                                               >  2:4374540/1‑140 (MQ=255)
                                          gTAGGCACTGTTTTAGGCGTAGCGCTGTTTCGCGGCGTACCGGTTGGGCCGCTTATTGCCGCGGGTCTGGTTTCGCTGATTGTGGGGAAACAGTAGTTACTCTCGCAATATATCGGCCTGGCGTGTGCCCCAGGCCTTTc                                                                                              <  2:131827/140‑1 (MQ=255)
                                            aGGCACTGTTTTAGGCGTAGCGCTGTTTCGCGGCGTACCGGTTGGGCCGCTTATTGCCGCGGGTCTGGTTTCGCTGATTGTGGGGAAACAGTAGTTAATCTCGCAATATATCGGCCTGGCGTTTGCCCCAGGCCTTTCtt                                                                                            <  1:2854701/140‑1 (MQ=255)
                                             ggCCCTGTTGTAGGCGTAGCGCTGTGTCGCGGCGTACCGGTTGGGCCGCTTATTGCCGCGGGTCTGGTTTCGCTGATTGTGGGGAAACAGTAGTTAATCTCGCAATATATCGGCCTGGCGTTTGCACCAGGCCTTTCTTa                                                                                           <  2:6531365/140‑1 (MQ=255)
                                             ggCACTTTTTTAGGCGTAGCGCTGTTTCGCGGCGTACCGGTTGGGCCGCTTATAGCCGCGGGTCTGGTTTCGCTGATTGTGGGGAAACAGTAGTTAATCTCGCAATATATCGGCCTGGCGTTTGCCCCAGGCCTTTCTTa                                                                                           <  2:4957559/140‑1 (MQ=255)
                                               cACTGTTTTAGGCGTAGCGCTGTTTCGCGGCGTACCGGTTGGGCCGCTTATTGCCGCGGGTCTGGTTTCGCTGATTGTGGGGAAACAGTAGTTAATCTCGCAATATATCGGCCTGGCGTTTGCCCCAGGCCTTTCTTaaa                                                                                         <  1:5459191/140‑1 (MQ=255)
                                               cACTGTTTTAGGCGTAGCGCTGTTTCGCGGCGTACCGGTTGGGCCGCTTATTGCCGCGGGTCTGGTTTCGCTGATTGTGGGGAAACAGTAGTTAATCTCGCAATATATCGGCCTGGCGTTTGCCCCAGGCCTTTCTTaaa                                                                                         <  2:718450/140‑1 (MQ=255)
                                               cACTGTTTTAGGCGTAGCGCTGTTTCGCGGCGTACCGGTTGGGCCGCTTATTGCCGCGGGTCTGGTTTCGCTGATTGTGGGGAAACAGTAGTTAATCTCGCAATATATCGGCCTGGCGTTTGCCCCAGGCCTTTCTTaaa                                                                                         <  2:1608208/140‑1 (MQ=255)
                                               cACTGTTTTAGGCGTAGCGCTGTTTCGCGGCGTACCGGTTGGGCCGCTTATTGCCGCGGGTCTGGTTTCGCTGATTGTGGGGAAACAGTAGTTAATCTCGCAATATATCGGCCTGGCGTTTGCCCCAGGCCTTTCTTaaa                                                                                         <  1:8208335/140‑1 (MQ=255)
                                                 cTGTTTTAGGCGTAGCGCTGTTTCGCNGCGTACCNGTTGGGCCGCTTATTGCCGCGGGTCTGGTTTCGCTGATTGTGGGGAAACAGTAGTTAATCTCGCAATATATCGGCCTGGCGTTTNCCCCAGCCCTTTCTTAAACa                                                                                       <  1:5531761/140‑1 (MQ=255)
                                                 cTGTTTTAGGCGTAGCGCTGTTTCGCGGCGTACCGGTTGGGCCGCTTATTGCCGCGGGTCTTGTTTCGCTGATTGTGGGGAAACAGTAGTTAATCTCGCAAGATATCGGCCTGGCGTTTGCCCCAGGCCTTTCTTAAACa                                                                                       <  2:8008743/140‑1 (MQ=255)
                                                 cTGTTTTAGGCGTAGCGCTGTTTCGCGGCGTACCGGTTGGGCCGCTTATTGCCGCGGGTCTGGTTTCGCTGATTGTGGGGACACAGTAGTTCTTCTCGCACTATATCGGCCTGGCGTTTGCCCCTGGCCTTTCTTATACa                                                                                       >  2:6132058/1‑140 (MQ=255)
                                                 cTGTTTTAGGCGTAGCGCTGTTTCGCGGCGTACCGGTTGGGCCGCTTATTGCCGCGGGTCTGGTTTCGCTGATTGTGGGGAAACAGTAGTTAATCTCGCAATATATCGGCCTGGCGTTTGCCCCAGGCCTTTCTTAAACa                                                                                       <  2:6698245/140‑1 (MQ=255)
                                                 cTGTTTGAGGCGTAGCGCTGTGTCGCGGCGTACCGGTTGGGCCGCTTATTGCCGCGGGTCTGGTTTCGCTGATTGTGGGGAAACAGTAGTTAATCTCGCAATATATCGGCCTGGCGTTTGCCCCAGGCCTTTCTTAAACa                                                                                       <  1:8568039/140‑1 (MQ=255)
                                                  tGTTTTAGGCGTAGCGCTGTTTCGCGGCGTACCGGTTGGGCCGCTTATTGCCGCGGGTCTGGTTTCGCTGATTGTGGGGAACCAGTAGTTAATCTCGCAATATATCGGCCTGGCGTTTGCCCCAGGCCTTTCTTAAACAt                                                                                      >  1:1760336/1‑140 (MQ=255)
                                                  tGTTTTAGGCGTAGCGCTGTTTCGCGGCGTACCGGTTGGGCCGCTTATTGCCGCGGGTCTGGTTTCGCTGATTGTGGGGAAACAGTAGTTAATCTCGCAATATATCGGCCTGGCGTTTGCCCCAGGCCTTTCTTAAACAt                                                                                      >  1:84351/1‑140 (MQ=255)
                                                  tGTTTTAGGCGTAGCGCTGTTTCGCGGCGTACCGGTTGGGCCGATGATTGCCGCGGGTCTGGTTTCGCTGATTGTGGGGAAACAGTAGTTAATCTCGCAATATATCGGCATGGCGTTTGCCCCAGGACTTTCTTAAACAt                                                                                      <  2:624341/140‑1 (MQ=255)
                                                   gTTTTAGGCGTAGCGCTGTTTCGCGGCGTACCGGTTGGGCCGCTTATTGCCGCGGGTCTGGTTTCGCTGATTGTGGGGAAACAGTAGTTAATCTCGCAATATATCGGCCTGGCGTTTGCCCCAGGCCTTTCTTAAACATa                                                                                     <  2:1736519/140‑1 (MQ=255)
                                                    ttttAGGCGTAGCGCTGTTTCGCGGCGTACCGGTTGGGCCGCTTATTGCCGCGGGTCTGGTTTCGCTGATTGTGGGGAAACAGTAGTTAATCTCGCAATATATCGGCCTGGCGTTTGCCCCAGGCCTTTCTTAAACATAg                                                                                    >  1:8071923/1‑140 (MQ=255)
                                                    ttttAGGCGTAGCGCTGTTTCGCGGCGTACCGGTTGGGCCGCTTATTGCCGCGGGTCTGGTTTCGCTGATTGTGGGGAAACAGGAGTTAATCTCGCAATATATCGGCCTGGCGTTTGCCCCAGGCCTTTCTTAAACATAg                                                                                    >  1:2092874/1‑140 (MQ=255)
                                                         ggCGTAGCGCTGTTTCGCGGCGTACCGGTTGGGCCGCTTATTGCCGCGGGTCTGGTTTCGCTGATTGTGGGGAAACAGTAGTTAATCTCGCAATATATCGGCCTGGCGTTTGCCCCAGGCCTTTCTTAAACATAGtaata                                                                               <  1:1174377/140‑1 (MQ=255)
                                                         ggCGTAGCGCTGTTTCGCGGCGTACCGGTTGGGCCGCTTATTGCCGCGGGTCTGGTTTCGCTGATTGTGGGGAAACAGTAGTTAATCTCGCAATATATCGGCCTGGCGTTTGCCCCAGGAGTTTCTTAAACATAGtaata                                                                               >  2:9439669/1‑140 (MQ=255)
                                                             tAGCGCTGTTTCGCGGCGTACCGGTTGGGCCGCTTATTGCCGCGGGTCTGGTTTCGCTGATTGTGGGGAAACAGTAGTTAATCTCGCAATATATCGGCCTGGCGTTTGCCCCAGGCCTTTCTTAAACATAGTAATAAAgg                                                                           <  1:6118760/140‑1 (MQ=255)
                                                              aGCGCTGTTTCGCGGCTTACCGGTTGGGCCGCTTATTGCCGCGGGTCTGGTTTCGCTGATTGTGGGGAAACAGTAGTTAATCTCGCAATATATCGGCCTGGCGTTTGCCCCAGGCCTTTCTTAAACATAGTAATAAAGGc                                                                          >  1:4247109/1‑140 (MQ=255)
                                                              aGCGCTGTTTCGCGGCGTACCGGTTGGGCCGCTTATTGCCGCGGGTCTGGTTTCGCTGATTGTGGGGAAACAGTAGTTAATCTCGCAATATATCGGCCTGGCGTTTGCCCCAGGCCTTTCTTAAACATAGTAATaaatga                                                                          >  1:4705644/1‑137 (MQ=255)
                                                              aGCGCTGTTTCGCGGCGTACCGGTTGGGCCGCTTATTGCCGCGGGTCTGGTTTCGCTGATTGTGGGGAAACAGTAGTTAATCTCGCAATATATCGGCCTGGCGTTTGCCCCAGGCCTTTCTTAAACATAGTAATAAAGGc                                                                          >  2:5163340/1‑140 (MQ=255)
                                                              aGCGCTGTTTCGCGGCGTACCGGTTGGGCCGCTTATTGCCGCGGGTCTGGTTTCGCTGATTGTGGGGAAACAGTAGTTAATCTCGCAATATATCGGCCTGGCGTTTGCCCCAGGCCTTTCTTAAACATAGTAATAAAGGc                                                                          >  1:2452915/1‑140 (MQ=255)
                                                              aGCGCTGTTTCGCGGCGTACCGGTTGGGCCGCTTATTGCCGCGGGTCTGGTTTCGCTGATTGTGGGGAAACAGTAGTTAATCTCGCAATATATCGGCCTGGCGTTTGCCCCAGGCCTTTCTTAAACATAGTAATAAAGGc                                                                          >  2:1481295/1‑140 (MQ=255)
                                                              aGCGCTGTTTCGCGGCGTACCGGTTGGGCCGCTTATTGCCGCGGGTCTGGTTTCGCTGATTGTGGGGAAACAGTAGTTAATCTCGCAATATATCGGCCTGGCGTTTGCCCCAGGCCTGTCTTAAACATAGTAATAAAGGc                                                                          >  1:5392122/1‑140 (MQ=255)
                                                              aGCGCTGTTTCGCGGAGTACCGGTTGGGCCGCTTATTGCCGAGGGTCTGGTTTCGCTGATTGTGGGGAAAAATTAGTTATTCTCGCAATATTTCTGCCTGGCGTTTGCCCCAGGCCTTTCTTAAACATAGTTATAAAgtc                                                                          <  2:1830376/140‑3 (MQ=255)
                                                                cgcTGTTTCGCGGCGTACCGGTTGGGCCGCTTATTGCCGCGGGTCTGGTTTCGCTGATTGTGGGGAAACAGTAGTTAATCTCGCAATATATCGGCCTGGCGTTTGCCCCAGGCCTTTCTTAAACATAGTAATAAAGGCCg                                                                        >  2:3515033/1‑140 (MQ=255)
                                                                    gTTTCGCGGCGTACCGGTTGGGCCGCTTATTGCCGCGGGTCTGGTTTCGCTGATTGTGGGGAAACAGTAGTTAATCTCGCAATATATCGGCCTGGCGTTTGCCCCAGGCCTTTCTTAAACATAGTAATAAAGGCCgttgt                                                                    <  2:6386381/140‑1 (MQ=255)
                                                                    gTTTCGCGGCGGACCGGTTGGGCCGCTTATTGCCGCGGGTCTGGTTTCGCTGATTGTGGGGAAACAGTAGTTAATCTCGCAATATATCGGCCTGGCGTTTGCCCCAGGCCTTTCTTAAACATAGTAATAAAGGCAgttgt                                                                    >  2:7598023/1‑140 (MQ=255)
                                                                     tttCGCGGCGTACCGGTAGGGCCGCTTATAGCCGCGGGTCTGGTTTCGCTGATTGTGGGGAAACAGTAGTTAACCTCGCAATATATCGGCCTGGCGTTTGCCCCAGGCCTTTCTTAAACATAGTAATAAAGGCCgttgtt                                                                   <  1:7883776/140‑1 (MQ=255)
                                                                      ttCGCGGCGTACCGGTTGGGCCGCTTATTGCCGCGGGTCTGGTTTCGCTGATTGTGGGGAAACAGTAGTTAATCTCGCAATATATCGGCCTGGCGTTTGCCCCAGGCCTTTCTTAACCATAGTAATAAAGGCCGttgttg                                                                  <  1:2252704/140‑1 (MQ=255)
                                                                        cgcgGCGTACCGGTTGGGCCGCTTATTGCCGCGGGTCTGGTTTCGCTGATTGTGGGGAAACAGTAGTTAATCTCGCAATATATCGGCCTGGCGTTTGCCCCAGGCCTTTCTTAAACATAGTAATAAAGGCCGTTGTTGaa                                                                <  1:4374540/140‑1 (MQ=255)
                                                                            gcgTACCGGTTGGGCCGCTTATTGCCGCGGGTCTGGTTTCGCTGATTGTGGGGAAACAGTAGTTAATCTCGCAATATATCGGCCTGGCGTTTGCCCCAGGCCTTTCTTAAACATAGTAATAAAGGCCGTTGTTGAATCAt                                                            <  1:5630784/140‑1 (MQ=255)
                                                                               tACCGGTTGGGCCGCTTATTGCCGCGGGTCTGGTTTCGCTGATTGTGGGGAAACAGTAGTTAATCTCGCACTATATCGGCCTGGCGTTTGCCCCAGGCCTTTCTTAAACATAGTAATAAAGGACGTTGTTGACTCATAcc                                                         >  2:294440/1‑140 (MQ=255)
                                                                                 ccGGTTGGGCCGCTTATTGCCGCGGGTCTGGTTTCGCTGATTGTGGGGAAACAGTAGTTAATCTCGCAATATATCGGCCTGGCGTTTGCCCCAGGCCTTTCTTAAACATAGTAATAAAGGCCGTTGTTGAATCATAcccc                                                       <  1:305062/140‑1 (MQ=255)
                                                                                 ccGGTTGGGCCGCTTAGTGCCGCGGGTCTGGTTTCGCTGATTGTGGGGAAACAGTAGTTAATCTCGCAATATATCGGCCTGGCGTTTGCCCCAGGCCTTTCTTAAACATAGTAATAAAGGCCGTTGTTGAATCATAcccc                                                       <  2:6865726/140‑1 (MQ=255)
                                                                                  cGGTTGGGCCGCTTAGTGCCGCGGGTCTGGTTTCGCTGATTGTGGGGAAACAGTAGTTAATCTCGCAATATATCGGCCTGGCGTTTGCCCCAGGCCTTTCTTAAACATAGTAATAAAGGCCGTTGTTGAATCATACCCCa                                                      <  1:518084/140‑1 (MQ=255)
                                                                                    gTTGGGCCGCTTATTGCCGCGGGTCTGGTTTCGCTGATTGTGGGGAAACAGTAGTTAATCTCGCAATATATCGGCCTGGCGTTTGCCCCAGGCCTTTCTTAAACATAGTAATAAAGGCCGTTGTTGAATCATACCCCAGc                                                    <  1:474392/140‑1 (MQ=255)
                                                                                        ggCCGCTTATTGCCGCGGGTCTGGTTTCGCTGATTGTGGGGAATCAGTAGTTAATCTCGCAATATATCGGCCTGGCGTTTGCCCCAGGCCTTTCTTAAACATAGTAATAAAGGCCGTTGTTGAATCATACCCCAGCGTAt                                                <  1:4433351/140‑1 (MQ=255)
                                                                                        ggCCGCTTATTGCCGCGGGTCTGGTTTCGCTGATTGTGGGGAAACAGTAGTTAATCTCGCAATATATCGGCCTGGCGTTTGCCCCAGGCCTTTCTTAAACATAGTAATAAAGGCCGTTGTTGAATCATACCCCAGCGTAt                                                <  2:9310713/140‑1 (MQ=255)
                                                                                         gCCGCTTATTGCCGCGGGTCTGGTTTCGCTGATTGTGGGGAAACAGTAGTTAATCTCGCAATATATCGGCCTGGCGTTTGCCCCAGGCCTTTCTTAAACATAGTAATAAAGGCCGTTGTTGAATCATACCCCAGCGTATg                                               >  1:4065972/1‑140 (MQ=255)
                                                                                         gCCGCTTATTGCCGCGGGTCTGGTTTCGCTGATTGTGGGGAAACAGTAGTTAATCTCGCAATATATCGGCCTGGCGTTTGCCCCAGGCCTTTCTTAAACATAGTAATAAAGGCCGTTGTTGAATCATACCCCAGCGTATg                                               >  2:5992208/1‑140 (MQ=255)
                                                                                          ccGCTTATTGCCGCGGGTCTGGGTTCGCTGATTGTGGGGAAACAGTAGTTAATCTCGCAATATATCGGCCTGGCGTTTGCCCCAGGCCTTTCTGAAACATAGTCCTAAAGGCCGTTGTTGAATCATACCCCAGCGTATGa                                              >  2:3035121/1‑140 (MQ=255)
                                                                                             cTTATTGCCGCGGGTCTGGTTTCGCTGATTGTGGGGACACAGTAGTTAATCTCGCAATATATCGGCCTGGCGTTTGCCCCAGGCCTTTCTTAAACAGAGTAATAAAGGCCGTTGTTGAATCATACCCCAGCGTATGAGCa                                           <  1:6383141/140‑1 (MQ=255)
                                                                                             cTTATTGCCGCGGGTCTGGTTTCGCTGATTGTGGGGAAACAGTAGTTAATCTCGCAATATATCGGCCTGGCGTTTGCCCCAGGCCTTTCTTAAACATAGTAATACAGGCCGTTGTTGACTCATACCCCAGCGTATGAGCa                                           <  2:2836419/140‑1 (MQ=255)
                                                                                             cTTATTGCCGCGGGTCTGGTTTCGCTGATTGTGGGGAAACAGTAGTTAATCTCGCAATATATCGGCCTGGCGTTTGCCCCAGGCCTTTCTTAAACATAGTAATAAAGGCCGTTGTTGAATCATACCCCAGCGTATGAGCa                                           <  1:9268802/140‑1 (MQ=255)
                                                                                             cTTATTGCCGCGGGTCTGGTTTCGCTGATTGTGGGGAAACAGTAGTTAATCTCGCAATATATCGGCCTGGCGTTTGCCCCAGGCCTTTCTTAAACATAGTAATAAAGGCCGTTGTTGAATCATACCCCAGCGTATGAGCa                                           <  1:7587060/140‑1 (MQ=255)
                                                                                             cTTATTGCCGCGGGTCTGGTTTCGCTGATTGTGGGGAAACAGTAGTTAATCTCGCAATATATCGGCCTGGCGTTTGCCCCAGGCCTTTCTTAAACATAGTAATAAAGGCCGTTGTTGAATCATACCCCAGCGTATGAGCa                                           <  1:7980335/140‑1 (MQ=255)
                                                                                               tATTGCCGCGGGTCTGGTTTCGCTGATTGTGGGGAAACAGTAGTTAATCTCGCAATATATCGGCCTGGCGTTTGCCCCAGGCCTTTCTTAACCATAGTAATAAAGGCCGTTGTTGAATCATACCCCAGCGTATGAGCAAc                                         <  1:675857/140‑1 (MQ=255)
                                                                                               tATTGCCGCGGGTCTGGTTTCGCTGATTGTGGGGAAACAGTAGTTAATCTCGCAATATATCGGCCTGGCGTTTGCCCCAGGCCTTTCTTAAACATAGTAATAAAGGCCGTTGTTGAATCATACCCCAGCGTATGAGCAAc                                         <  1:389702/140‑1 (MQ=255)
                                                                                                aTTGCCGCGGGTCTGGTTTCGCTGATTGTGGGGAAACAGTAGTTAATCTCGCAATATATCGGCCTGGCGTTTGCCCCAGGCCTTTCTTAAACATAGTAATAAAGGCCGTTGTTGAATCATACCCCAGCGTATGAGCAACt                                        <  1:9427339/140‑1 (MQ=255)
                                                                                                       cgGGTCTGGTTTCGCTGTTTGTGGGGAAACAGTAGTTAATCTCTCAATATATCGGCCTGGCTTTTGCCCCAGGCCTTTCTTAAACATAGTAATAAATGCCGTTGTTGAATCATACCCCAGCGTATGAGCAACTTTCTGTa                                 <  2:2009711/140‑1 (MQ=255)
                                                                                                       cgGGTCTGGTTTCGCTGATTGTGGGGAAACAGTAGTTAATCTCGCAATATATCGGCCTGGCGTTTGCCCCAGGCCTTTCTTAAACATAGTAATAAAGGCCGTTGTTGAATCATACCCCAGCGTATGAGCAACTTTCTGTa                                 <  2:7256037/140‑1 (MQ=255)
                                                                                                       cgGGTCTGGTTTCGCTGATTGTGGGGAAACAGTAGTTAATCTCGCAATATATCGGCCTGGCGTTTGCCCCAGGCCTTTCTTAAACATAGTAATAAAGGCCGTTGTTGAATCATACCCCAGCGTATGAGCAACTTTCTGTa                                 >  1:5282581/1‑140 (MQ=255)
                                                                                                          gTCTGGTTTCGCTGATTGTGGGGAAACAGTAGTTAATCTCGCAATATATCGGCCTGGCGTTTGCCCCAGGCCTTTCTTAAACATAGTAATAAAGGCCGTTGTTGAATCATACCCCAGCGTATGAGCAACTTTCTGTACAg                              <  1:6526077/140‑1 (MQ=255)
                                                                                                            cTGGTTTCGCTGATTGTGGGGAAACAGTAGTTAATCTCGCAATATATCGGCCTGGCGTTTGCCCCAGGCCTTTCTTAAACATAGTAATAACGGCCGTTGTTGAATCATACCCCAGCGTATGAGCAACTTTCTGTACAGTa                            <  1:8770164/140‑1 (MQ=255)
                                                                                                            cTGGTTTCGCTGATTGTGGGGAAACAGTAGTTAATCTCGCAATATATCGGCCTGGCGTTTGCCCCAGGCCTTTCTTAAACATAGTAATAAAGGCCGTTGTTGAATCATACCCCAGCGTATGAGCAACTTTCTGTACAGTa                            <  1:2706426/140‑1 (MQ=255)
                                                                                                             tGGTTTCGCTGATTGTGGGGAAACAGTAGTTAATCTCGCAATATATCGGCCTGGCGTTTGCCCCAGGCCTTTCTTAAACATAGTAATAAAGGCCGTTGTTGAATCATACCCCAGCGTATGAGCAACTTTCTGTACAGTAt                           <  1:3282557/140‑1 (MQ=255)
                                                                                                             tGGTTTCGCTGATTGTGGGGAAACAGTAGTTAATCTCGCAATATATCGGCCTGGCGTTTGCCCCAGGCCTTTCTTAAACATAGTAATAAAGGCCGTTGTTGAATCATACCCCAGCGTATGAGCAACTTTCTGTACAGTAt                           <  1:7186732/140‑1 (MQ=255)
                                                                                                                      tGATTGTGGGGAAACAGTAGTTAATCTCGCAATATATCGGCCTGGCGTTTGCCCCAGGCCTTTCTTAAACATAGTAATAAAGGCCGTTGTTGAATCATACCCCAGCGTATGAGCAACTTTCTGTACAGTATCGCCTTTCa                  <  1:4189578/140‑1 (MQ=255)
                                                                                                                             ggggAAACAGTAGTTAATCTCGCAATATATCGGCCTGGCGTTTGCCCCCGGCCTTTATTAAACATATTAATAAATGCCGTTGTTGAATAATACCCCAGCGTATGAGCAACATTCTGTACAGTATCGCCTTTCACTAATcc           >  1:1871052/1‑140 (MQ=255)
                                                                                                                                  aaCAGTAGTTAATCTCGCAATATATCGGCCTGGCGTTTGCCCCAGGCCTTTCTTAAACATAGTAATAAAGGCCGTTGTTGAATCATACCCCAGCGTATGAGCAACTTTCTGTACAGTATCGCCTTTCACTAATCCCTGaa      <  1:921982/140‑1 (MQ=255)
                                                                                                                                    cAGTAGATAATCTCGCAATATATCGGCCTGGCGTTTGCCCCAGGCCTTTCTTAAACATAGTAATAAAGGCCGTTGTTGAATCATACCCCAGCGTATGAGCAACTTTCTGTACAGTATCGCCTTTCACTAATCCCTGactc    >  2:5476355/1‑137 (MQ=255)
                                                                                                                                         |                                                                                                                                        
ACGTTAATGGGCAGCCAGCCGCAACTGGTCGCCGGGCTGCTGGTAGGCACTGTTTTAGGCGTAGCGCTGTTTCGCGGCGTACCGGTTGGGCCGCTTATTGCCGCGGGTCTGGTTTCGCTGATTGTGGGGAAACAGTAATTAATCTCGCAATATATCGGCCTGGCGTTTGCCCCAGGCCTTTCTTAAACATAGTAATAAAGGCCGTTGTTGAATCATACCCCAGCGTATGAGCAACTTTCTGTACAGTATCGCCTTTCACTAATCCCTGAAGCGC  >  NZ_CP025268/1879475‑1879748

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑