BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP025268	2,514,921	A→G	419 (TAA→TAG)	yfeO →	ion channel protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP025268	2,514,921	0	A	G	99.4%	2189.2 / NA	640	419 (TAA→TAG)	yfeO	ion channel protein
Reads supporting (aligned to +/- strand): ref base A (0/0); major base G (329/307); minor base T (0/4); total (329/311)
Fisher's exact test for biased strand distribution p-value = 5.52e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 640 total aligned reads displayed.

GCGGCAGTCGTTGTACCCAATACCACATTGCTACCGCTGCTCTGTATCGTCATGCTTCCGGCATGGCTGTTATTAGCAGGTAAGCCGATGATGATGGTCAATCGTCCGAAGCAACAGCCACCCCACGATAACGTTTAACAAAAAATGCTCTCCTTTATGATTTAAGAGTTATGGCGATGATACGGGGCTTTACCTCCCCGTAATATTGCGTTAACAGGCCGCTGACGGCTTCGAGCGTGAAGGAGAATAACAATGTTCAGGTCACTGTTTCTGG  >  NZ_CP025268/2514784‑2515057

  ggcAGTCGTTGTACCCAATACCACATTGCTACCGCTGCTCTGTATCGTCATGCTTCCGGCATGGCTGTTATTAGCAGGTAAGCCGATGATGATGGTCAATCGTCCGAAGCAACAGCCACCCCACGATAACGTTTAGCaaa                                                                                                                                      >  1:2916453/1‑140 (MQ=255)

  ggcAGTCCTAGTACCCAAGACCACATGGCTACCGCTGCTCTGTCTCGTCATGCTTCCGGCATGGCTGTTATTAGCAGGTAAGCCGATGATGATGGTCAATCGTCCGAAGCAACAGCCACCCCACGATAACGATTAGCaaa                                                                                                                                      <  2:6106175/140‑1 (MQ=255)

    ccgTCGTTGTACCCAATACCACATTGCTACCGCTGCTCTGTATCGTCATGCTTCCGGCATGGCTGTTATTAGCAGGTAAGCCGATGATGATGGTCAATCGTCCGAAGCAACAGCCACCCCACGATAACGTTTAGCaaaaa                                                                                                                                    >  2:3351204/3‑140 (MQ=255)

         tttGTACCCAATACCACATTGCTACCGCTGCTCTTTATCGTCATGCTTCAGGCATGGCTGTTATTAGCAGGTAAGCCGATGATGATTGTAAATCGTCCGAAGCAACAGCCACCCAACGATAACGTTTAGCAAAAATTGct                                                                                                                               <  1:2346450/139‑1 (MQ=255)

         gTTGTACCCAATACCACATTGCTACCGCTGCTCTGTATCGTCATGCTTCCGGCATGGCTGTTATTAGCAGGTAAGCCGTTGATGATGGTCAATCGTCCGAAGCAACAGCCACCCCACGATAACGTTTAGCAAAAAATGct                                                                                                                               <  2:5340525/140‑1 (MQ=255)

         gTTGTACCCAATACCACATTGCTACCGCTGCTCTGTATCGTCATGCTTCCGGCATGGCTGTTATTAGCAGGTAAGCCGATGATGATGGTCAATCGTCCGAAGCAACAGCCACCCCACGATAACGTTTAGCAAAAAATGct                                                                                                                               <  2:4541857/140‑1 (MQ=255)

         gTTGTACCCAATACCACATTGCTACCGCTGCTCTGTATCGTCATGCTTCCGGCATGGCTGTTATTAGCAGGTAAGCCGATGATGATGGTCAATCGTCCGAAGCAACAGCCACCCCACGATAACGTTTAGCAAAAAATGct                                                                                                                               <  1:9499651/140‑1 (MQ=255)

         gTTGTACCCAATACCACATTGCTACCGCTGCTCTGTATCGTCATGCTTCCGGCATGGCTGTTATTAGCAGGTAAGCCGATGATGATGGTCAATCGTCCGAAGCAACAGCCACCCCACGATAACGTTTAGCAAAAAATGct                                                                                                                               <  1:8182641/140‑1 (MQ=255)

         gTTGTACCCAATACCACATTGCTACCGCTGCTCTGTATCGTCATGCTTCCGGCATGGCTGTTATTAGCAGGTAAGCCGATGATGATGGTCAATCGTCCGAAGCAACAGCCACCCCACGATAACGTTTAGCAAAAAATGct                                                                                                                               <  1:2864441/140‑1 (MQ=255)

         gTTGTACCCAATACCACATTGCTACCGCTGCTCTGTATCGTCATGCTTCCGGCAGGGCTGTTATTAGCAGGTACGCCGATGATGATGGTCAATCGTCCGAAGCAACAGCCACCCCACGATACCGTTTAGCAAAAAATGct                                                                                                                               <  2:2888219/140‑1 (MQ=255)

          ttGTACCCAATACCACATTGCTACCGCTGCTCTGTATCGTCATGCTTCCGGCATGGCTGTTATTAGCAGGTAAGCCGATGATGATGGTCAATCGTCCGAAGCAACAGCCACCCCACGATAACGTTTAGCAAAAAATGctc                                                                                                                              <  1:9008446/140‑1 (MQ=255)

          ttGTACCCAATACCACATTGCTACCGCTGCTCTGTATCGTCATGCTTCCGGCATGGCTGTTATTAGCAGGTAAGCCGATGATGATGGTCAATCGTCCGAAGCAACAGCCACCCCACGATAACGTTTAGCAAAAAATGctc                                                                                                                              <  1:311535/140‑1 (MQ=255)

                 cAATACCACATTGCTCCCGCTGCTCTGTATCGTCATGCTTCCGGCATGGCTGTTATTAGCAGGTAAGCCGATGATGATGGTCAATCGTCCGAAGCAACAGCCACCCCACGATAACGTTTAGCAAAAAATGCTCTCCTTTa                                                                                                                       <  1:5195532/140‑1 (MQ=255)

                 cAATACCACATTGCTACCGCTGCTCTGTATCGTCATGCTTCCGGCATGGCTGTTATTAGCAGGTAAGCCGATGATGATGGTCAATCGTCCGAAGCAACAGCCACCCCACGATAACGTTTAGCAAAAAATGCTCTCCTTTa                                                                                                                       <  1:6298031/140‑1 (MQ=255)

                  aaTACCACATTGCTACCGCTGCTCTGTATCGTCATGCTTCCGGCATGGCTGTTATTAGCAGGTAAGCCGATGATGATGGTCAATCGTCCGAAGCAACAGCCACCCCACGATAACGTTTAGCAAAAAATGCTCTCCTTTAt                                                                                                                      >  1:7443337/1‑140 (MQ=255)

                  aaTACCACATTGCTACCGCTGCTCTGTATCGTCATGCTTCCGGCATGGCTGGTATTAGCAGGTAAGCCGATGATGATGGTCAATCGTCCGAAGCAACAGCCACCCCACGATAACGTTTAGCAAAAAATGCTCTCCTTTAt                                                                                                                      >  1:6229557/1‑140 (MQ=255)

                    tACCACATTGCTACCGCTGCTCTGTATCGTCATGCTTCCGGCATGGCTGTTATTAGCAGGTAAGCCGATGATGATGGTCAATCGTCCGAAGCAACAGCCACCCCACGATCACGTTTAGCAAAAAATGCTCTCCTTTATGa                                                                                                                    >  2:1994685/1‑140 (MQ=255)

                    tACCACATTGCTACCGCTGCTCTGTATCGTCATGCTTCCGGCATGGCTGTTATTAGCAGGTAAGCCGATGATGATGGTCAATCGTCCGAAGCAACAGCCACCCCACGATAACGTTTAGCAAAAAATGCTCTCCTTTATGa                                                                                                                    >  2:4738570/1‑140 (MQ=255)

                       cacaTTGCTACCGCTGCTCTGTATCGTCATGCTTCCGGCATGGCTGTTATTAGCAGGTAAGCCGATGATGATGGTCAATCGTCCGAAGCAACAGCCACCCCACGATAACGTTTAGCAAAAAATGCTCTCCTTTATGAttt                                                                                                                 >  1:3686270/1‑140 (MQ=255)

                       cacaTTGCTACCGCTGCTCTGTATCGTCATGCTTCCGGCATGGCTGTTATTAGCAGGTAAGCCGATGATGATGGTCAATCGTCCGAAGCAACAGCCACCCCACGATAACGTTTAGCAAAAAATGCTCTCCTTTATGAttt                                                                                                                 >  1:5696935/1‑140 (MQ=255)

                         caTTGCTACCGCTGCTCTGTATCGTCATGCTTCCGGCATGGCTGTTATTAGCAGGTAAGCCGATGATGATGGTCAATCGTCCGAAGCAACAGCCACCCCACGATAACGTTTAGCAAAAAATGCTCTCCTTTATGATTTaa                                                                                                               >  2:3693296/1‑140 (MQ=255)

                            tGCTACCGCTGCTCTGTATCGTCATGCTTCCGGCATGGCTGTTATTAGCAGGTAAGCCGATGATGATGGTCAATCGTCCGAAGCAACAGCCACCCCACGATAACGTTTAGCAAAAAATGct                                                                                                                               <  1:7884302/121‑1 (MQ=255)

                            tGCTACCGCTGCTCTGTATCGTCATGCTTCCGGCATGGCTGTTATTAGCAGGTAAGCCGATGATGATGGTCAATCGTCCGAAGCAACAGCCACCCCACGATAACGTTTAGCAAAAAATGCTCTCCTTTATGATTTAagag                                                                                                            >  1:4055581/1‑140 (MQ=255)

                            tGCTACCGCTGCTCTGTATCGTCATGCTTCCGGCATGGCTGTTATTAGCAGGTAAGCCGATGATGATGGTCAATCGTCCGAAGCAACAGCCACCCCACGATAACGTTTAGCAAAAAATGCTCTCCTTTATGATTTAagag                                                                                                            >  1:2245872/1‑140 (MQ=255)

                            tGCTACCGCTGCTCTGTATCGTCATGCTTCCGGCATGGCTGTTATTAGCAGGTAAGCCGATGATGATGGTCAATCGTCCGAAGCAACAGCCACCCCACGATAACGTTTAGCAAAAAATGCTCTCCTATCTGATTTAagag                                                                                                            >  2:6369446/1‑140 (MQ=255)

                              cTACCGCTGCTCTGTATCGTCATGCTTCCGGCATGGCTGTTATTAGCAGGTAAGCCGATGATGATGGTCAATCGTCCGAAGCAACAGCCACCCCACGATAACGTTTAGCAAAAAATGCTCTCCTTTATGATTTAAGAGtt                                                                                                          >  1:268616/1‑140 (MQ=255)

                              cTACCGCTGCTCTGTATCGTCATGCTTCCGGCATGGCTGTTATTAGCAGGTAAGCCGATGATGATGGTCAATCGTCCGAAGCAACAGCCACCCCACGATAACGTTTAGCAAAAAATGCTCTCCTTTATGAGTTAAGAGtt                                                                                                          >  2:1408088/1‑140 (MQ=255)

                              cTACCGCTGCTCTGTATCGTCATGCTTCCGGCATGGCTGTTATTAGCAGGTAAGCCGATGATGATGGTCAATCGTCCGAAGCAACAGCCACCCCACGATAACGTTTAGCAAAAAATGCTCTCCTTTAGGATTTAAGAGtt                                                                                                          >  2:8538680/1‑140 (MQ=255)

                                agcGCTGCTCTGTATCGTCATGCTTCCGGCATGGCTGTTATTAGCAGGTAAGCCGATGATGATGGTCAATCGTCCGAAGCAACAGCCACCCCACGAGAACGTTTAGCAAAAAATGCTCTCCTTTATGATTTAAGAGTTAt                                                                                                        <  2:7049458/138‑1 (MQ=255)

                                aCCGCTGCTCTGTATCGTCATGCTTCCGGCATGGCTGTTATTAGCAGGTAAGCCGATTATGATGGTCAATCGTCCGAAGCAACAGCCACCCCACGATAACGTTTAGCAAAAAATGCTCTCCTTTATGATTTAAGAGTTAt                                                                                                        >  2:4073678/1‑140 (MQ=255)

                                aCCGCTGCTCTGTATCGTCATGCTTCCGGCATGGCTGTTATTAGCAGGTAAGCCGATGATGATGGTCAATCGTCCGAAGCAACAGCCACCCCACGATAACGTTTAGCAAAAAATGCTCTCCTTTATGATTTAAGAGTTAt                                                                                                        <  2:5183000/140‑1 (MQ=255)

                                  cGCTGCTCTGTATCGTCATGCTTCCGGCATGGCTGTTATTAGCAGGTAAGCCGATGATGATGGTCAATCGTCCGAAGCAACAGCCACCCCACGATAACGTTTAGCAAAAAATGCTCTCCTTTATGATTTAAGAGTTATgg                                                                                                      <  1:7262320/140‑1 (MQ=255)

                                    ctgctCTGTATCGTCATGCTTCCGGCATGGCTGTTATTAGCAGGTAAGCCGATGATGATGGTCAATCGTCCGAAGCAACAGCCACCCCACGATAACGTTTAGCAAAAAATGCTCTCCTTTATGATTTAAGAGTTATGGCg                                                                                                    <  2:5961162/140‑1 (MQ=255)

                                    ctgctCTGTATCGTCATGCTTCCGGCATGGCTGTTATTAGCAGGTAAGCCGATGATGATGGTCAATCGTCCGAAGCAACAGCCACCCCACGATAACGTTTAGCAAAAAATGCTCTCCGTTATGATTTAAGAGTTATGGCg                                                                                                    >  2:1638284/1‑140 (MQ=255)

                                    ctgctCTGTATCGTCATGCTTCCGGCATGGCTGTTATGAGCAGGTAAGCCGATGATGATGGTCAATCGTCCGAAGCAACAGCCACCCCACGATAACGGTTAGCAAAAAATGCTCTCCTTTAGGATTTAAGAGTTATGGCg                                                                                                    <  2:1894179/140‑1 (MQ=255)

                                    ctgatCTGTATCGTCATGCTTCCGGCATGGCTGTTATTAGCAGGTAAGCCGATGATGATGGTCAATCGTCCGAAGCAACAGCCACCCCACGATAACGTTTAGCAAAAAATGCACTCCTTTATGATTTAAGAGTTATGGCg                                                                                                    >  2:4207352/1‑140 (MQ=255)

                                     tgctCTGTATCGTCATGCTTCCGGCATGGCTGTTATTAGCAGGTAAGCCGATGATGATGGTCAATCGTCCGAAGCAACAGCCACCCCACGATAACGTTTAGCAAAAAATGCTCTCCTTTATGATTTAAGAGTTATGGCga                                                                                                   <  1:2566882/140‑1 (MQ=255)

                                      gctCTGTATCGTCATGCTTCCGGCATGGCTGTTATTAGCAGGTAAGCCGATGATGATGGTCAATCGTCCGAAGCAACAGCCACCCCACGATAACGTTTAGCAAAAAATGCTCTCCTTTATGATTTAAGAGTTATGGCgat                                                                                                  >  2:8862586/1‑140 (MQ=255)

                                      gctCTGTATCGTCATGCTTCCGGCATGGCTGTTATTAGCAGGTAAGCCGATGAGGATGGTCAATCGTCCGAAGCAACAGCCACCCCACGATAACGTTTAGCAAAAAATGCTCTCCGTTATGATTTAAGAGTTATGGCgat                                                                                                  >  1:1004364/1‑140 (MQ=255)

                                       ctctGTATCGTCATGCTTCCGGCATGGCTGTTATTAGCAGGTAAGCCGATGATGATGGTCAATCGTCCGAAGCAACAGCCACCCCACGATAACGTTTAGCAAAAAATGCTCTCCTTTATGATTTAAGAGTTATGGCgatg                                                                                                 >  1:7653958/1‑140 (MQ=255)

                                           gTATCGTCATGCTTCCGGCATTGATGTTTTTAGCAGGTAAGCCGATGATGATGGTCAATCGTCCGAAGCAACAGCCACCCCACGATAACGTTTATCAAAAAATGCTCTCCTTTATGATTTAAGAGTTATGGCGATGATAc                                                                                             <  2:8003937/140‑1 (MQ=255)

                                                 tCATGCTTCCGGCATGGCTGTTATTAGCAGGTAAGCCGATGATGATGGTCAATCGTCCGAAGCAACAGCCACCCCACGATAACGTTTAGCAAAAAATGCTCTCCTTTATGATTTAAGAGTTATGGCGATGATACGGGGCt                                                                                       >  2:5125099/1‑140 (MQ=255)

                                                 tCATGCTTCCGGCATGGCTGTTATTAGCAGGTAAGCCGATGATGATGGTCAATCGTCCGAAGCAACAGCCACCCCACGATAACGTTTAGCAAAAAATGCTCTCCTTTATGATTTAAGAGTTATGGCGATGATACGGGGCt                                                                                       >  1:5505885/1‑140 (MQ=255)

                                                     gCTTCCTGAATGGCTGTTATTAGCAGGTAAGCCGATGATGAGGGTCAATCGTCCGAAGCAACAGCCACCCCACGATAACGTTTAGCAAAAAATGCTCTCCTTTATGATTTAAGAGTTATGGCGATGATACGGGGCTTTAc                                                                                   <  1:2164332/140‑1 (MQ=255)

                                                     gCTTCCGGCATGGCTGTTATTAGCCGGTACGCCGATGATGATGGTCACTCGTCCGAAGCAACAGCCACCCCCCGAGAACGTTTAGCAAACAATGCTCTCCTTTATGATTTAAGAGTTATGGCGATGATACGGGGCTTTAc                                                                                   <  1:6473065/140‑1 (MQ=255)

                                                     gCTTCCGGCATGGCTGTTATTAGCAGGTAAGCCGATGATGATGGTCAATCGTCCGAAGCAACAGCCACCCCACGATAACGTTTAGCAAAAAATGCTCTCCTTTATGATTTAAGAGTTATGGCGATGATACGGGGCTTTAc                                                                                   <  2:6802461/140‑1 (MQ=255)

                                                      cTTCCGGCATGGCTGTTATTAGCAGGTAAGCCGATGATGATGGTCAATCGTCCGAAGCAACAGCCACCCCACGATAACGTTTAGCAAAAAATGCTCTCCTTTATGATTTAAGAGTTATGGCGATGATACGGGGCTTTAcc                                                                                  >  2:6954788/1‑140 (MQ=255)

                                                      cTTCCGGCATGGCTGTTATTAGCAGGTAAGCCGATGATGATGGTCAATCGTCCGAAGCAACAGCCACCCCACGATAACGTTTAGCAAAAAATGCTCTCCTTTATGATTTAAGAGTTATGGCGATGATACGGGGCTTTAcc                                                                                  >  2:7896939/1‑140 (MQ=255)

                                                      cTTCCGGCATGGCTGTTATTAGCAGGTAAGCCGATGATGATGGTCAATCGTCCGAAGCAACAGCCACCCCACGATAACGTTTAGCAAAAAATGCTCTCCTTTATGATTTAAGAGTTATGGCGATGATACGGGGCTTTAcc                                                                                  >  1:5350424/1‑140 (MQ=255)

                                                         ccGGCATGGCTGTTATTAGCAGGTAAGCCGATGATGATGGTCAATCGTCCGAAGCAACAGCCACCCCACGATAACGTTTAGCAAAAAATGCTCTCCTTTATGATTTAAGAGTTATGGCGATGATACGGGGCTTTACCTcc                                                                               <  1:4237032/140‑1 (MQ=255)

                                                         ccGGCATGGCTGTTATTAGCAGGTAAGCCGATGATGATGGTCAATCGTCCGAAGCAACAGCCACCCCACGATAACGTTTAGCAAAAAATGCTCTCCTTTATGATTTAAGAGTTATGGCGATGATACGGGGCTTTACCTcc                                                                               <  2:6480106/140‑1 (MQ=255)

                                                                ggCTGTTATTAGCAGGTAAGCCGATGATGATGGTCAATCGTCCGAAGCAACAGCCACCCCACGATAACGTTTAGCAAAAAATGCTCTCCTTTATGATTTAAGAGTTATGGCGATGATACGGGGCTTTACCTCCCCGTAat                                                                        <  1:8474904/140‑1 (MQ=255)

                                                                ggCTGTTATTAGCAGGTAAGCCGATGATGATGGTCAATCGTCCGAAGCAACAGCCACCCCACGATAACGTTTAGCAAAAAATGCTCTCCTTTATGATTTAAGAGTTATGGCGATGATACGGGGCTTTACCTCCCCGTAat                                                                        >  2:833201/1‑140 (MQ=255)

                                                                ggCTGTTATTAGCAGGTAAGCCGATGATGATGGTCAATCGTCCGAAGCAACAGCCACCCCACGATAACGTTTAGCAAAAAATGCTCTCCTGTATGATTTAAGAGTTATGGCGATGATACGGGGCTTTACCTCCCCGTaag                                                                        >  2:7594039/1‑139 (MQ=255)

                                                                ggCAGTTATTAGCAGGTAAGCCGACGATGATGGTCACTCGTCCGAAGCAACAGCCACCCCCCGATAACGTTTAGCAAAAAATGCTCTCCTTTATGATTTAAGAGTTATGGCGATGATACGGGGCTTTACCTCCCCGTAat                                                                        <  1:6712700/140‑1 (MQ=255)

                                                                 gCTGTTATTAGCAGGTAAGCCGATGATGATGGTCAATCGTCCGAAGCATCAGCCACCCCACGATAACGTTTAGCAAAAAATGCTCTCCTTTATGAGTTAAGAGTTATGGCGATGATCCGGGGGTTTACCTCCCCGtgaga                                                                       >  2:5800644/1‑136 (MQ=255)

                                                                 gCTGTTATTAGCAGGTAAGCCGATGATGATGGTCAATCGTCCGAAGCAACAGCCACCCCACGATAACGTTTAGCAAAAAATGCTCTCCTTTATGATTTAAGAGTTATGGCGATGATACGGGGCTTTACCTCCCCGTAata                                                                       >  1:9125285/1‑140 (MQ=255)

                                                                 gCTGTTATTAGCAGGTAAGCCGATGATGATGGTCAATCGTCCGAAGCAACAGCCACCCCACGATAACGTTTAGCAAAAAATGCTCTCCTTTATGATTTAAGAGTTATGGCGATGATACGGGGCTTTACCTCCCCGTAata                                                                       >  1:4449459/1‑140 (MQ=255)

                                                                  cTGTTATTAGCAGGTAAGCCGATGATGATGGTCAATCGTCCGAAGCAACAGCCACCCCACGATAACGTTTAGCAAAAAATGCTCTCCTGTATGATTTAAGAGTTATGGCGATGATACGGGGCTTTACCTCCCCGTAatat                                                                      >  1:3015337/1‑140 (MQ=255)

                                                                     ttattaGCAGGTAAGCCGATGATGATGGTCAATCGTCCGAAGCAACAGCCACCCCACGATAACGTTTAGCAAAAAATGCTCTCCTTTATGATTTAAGAGTTATGGCGATGATACGGGGCTTTACCTCCCCGTAATATTGc                                                                   >  1:4979948/1‑140 (MQ=255)

                                                                            cAGGTAAGCCGATGATGATGGTCAATCGTCCGAAGCAACAGCCACCCCACGATAACGTTTAGCAAAAAATGCTCTCCTTTATGATTTAAGAGTTATGGCGATGATACGGGGCTTTACCTCCCCGTAATATTGCGTTAACa                                                            >  1:6816297/1‑140 (MQ=255)

                                                                            cAGGTAAGCCGATGATGATGGTCAATCGTCCGAAGCAACAGCCACCCCACGATAACGTTTAGCAAAAAATGCTCTCCGTGAGGAGTTAAGAGTTATGGCGAGGATACGGGGCTTTACCTCCCCGTAATATTGCGTTAACa                                                            >  1:4681013/1‑140 (MQ=255)

                                                                              ggTAAGCCGATGATGATGGTCAATCGTCCGAAGCAACAGCCACCCCACGATAACGTTTAGCAAAAAATGCTCTCCTTTATGATTTAAGAGTTATGGCGATGATACGGGGCTTTACCTCCCCGTAATATTGCGTTAACAgg                                                          <  1:119477/140‑1 (MQ=255)

                                                                               gTAAGCCGATGATGATGGTCAATCGTCCGAAGCAACAGCCACCCCACGATAACGTTTAGCAAAAAATGCTCTCCTTTATGATTTAAGAGTTATGGCGATGATACGGGGCTTTACCTCCCCGTAATATTGCGTTAACAGGc                                                         >  1:6007252/1‑140 (MQ=255)

                                                                               gTAAGCCGATGATGATGGTCAATCGTCCGAAGCAACAGCCACCCCACGATAACGTTTAGCAAAAAATGCTCTCCTTTATGATTTAAGAGTTATGGCGATGATACGGGGCTTTACCTCCCCGTAATATTGCGTTAACAGGc                                                         >  1:4369007/1‑140 (MQ=255)

                                                                               gTAAGCCGATGATGATGGTCAATCGTCCGAAGCAACAGCCACCCCACGATAACGTTTAGCAAAAAATGCTCTCCTTTATGATTTAAGAGTTATGGCGATGATACGGGGCTTTACCTCCCCGGCATATTGCGTTAACAGGc                                                         >  1:7775003/1‑140 (MQ=255)

                                                                                  aGCCGATGATGATGGTCAATCGTCCGAAGCAACAGCCACCCCACGATAACGTTTAGCAAAAAATGCTCTCCTTTATGATTTAAGAGTTATGGCGATGATACGGGGCTTTACCt                                                                                 >  2:2453561/1‑113 (MQ=255)

                                                                                  aGCCGATGATGATGGTCAATCGTCCGAAGCAACAGCCACCCCACGATAACGTTTAGCAAAAAATGCTCTCCTTTATGATTTAAGAGTTATGGCGATGATACGGGGCTTTACCt                                                                                 <  1:2453561/113‑1 (MQ=255)

                                                                                            gatgGTCAATCGTCCGAAGCAACAGCCACCCCACGATAACGTTTAGCAAAAAATGCTCTCCTTTATGATTTAAGAGTTATGGCGATGATACGGGGCTTTACCTCCCCGTAATATTGCGTTAACAGGCCGCTGACGGCTTc                                            <  1:1448862/140‑1 (MQ=255)

                                                                                              tgGTCAATCGTCCGAAGCAACAGCCACCCCACGATAACGTTTAGCAAAAAATGCTCTCCTTTATGATTTAAGAGTTATGGCGATGATACGGGGCTTTACCTCCCCGTAATATTGCGTTAACAGGCCGCTGACGGCTTCGa                                          <  2:9247232/140‑1 (MQ=255)

                                                                                              tgGTCAATCGTCCGAAGCAACAGCCACCCCACGATAACGTTTAGCAAAAAATGCTCTCCTTTATGATTTAAGAGTTATGGCGATGATACGGGGCTTTACCTCCCCGTAATATTGCGTTAACAGGCCGCTGACGGCTTCGa                                          <  2:865292/140‑1 (MQ=255)

                                                                                              tgGTCAATCGTCCGAAGCAACAGCCACCCCACGATAACGTTTAGCAAAAAATGCTCTCCTGTATGATTTAAGAGTTATGGCGATGATACGGGGCTTTACCTCCCCGTAATATTGCGTTAACAGGCCGCTGACGGCTTCGa                                          >  2:2611046/1‑140 (MQ=255)

                                                                                                    aTCGTCCGAAGCAACAGCCACCCGAGGATAACGTTTAGCAAAAAATGCTCTCCTTTATGATTTAAGAGTTATGGCGATGATACGGGGCTTTACCTCCCCGTAATATTGCGTTAACAGGCCGCTGACGGCTTCGAGCGTGa                                    <  2:7121303/140‑1 (MQ=255)

                                                                                                    aTCGTCCGAAGCAACAGCCACCCCACGATAACGTTTAGCAAAAAATGCTCTCCTTTATGATTTAAGAGTTATGGCGATGATACGGGGCTTTACCTCCCCGTAATATTGCGTTAACAGGCCGCTGACGGCTTCGAGCGTGa                                    <  1:336383/140‑1 (MQ=255)

                                                                                                    aTCGTCCGAAGCAACAGCCACCCCACGATAACGTTTAGCAAAAAATGCTCTCCTTTATGATTTAAGAGTTATGGCGATGATACGGGGCTTTACCTCCCCGTAATATTGCGTTAACAGGCCGCTGACGGCTTCGAGCGTGa                                    <  1:8672172/140‑1 (MQ=255)

                                                                                                    aTCGTCCGAAGCAACAGCCACCCCACGATAACGTTTAGCAAAAAATGCTCTCCTTTATGATTTAAGAGTTATGGCGATGATACGGGGCTTTACCTCCCCGTAATATTGCGTTAACAGGCCGCTGACGGCTTCGAGCGTGa                                    <  2:3251784/140‑1 (MQ=255)

                                                                                                      cGTCCGAAGCAACAGCCACCCCACGATAACGTTTAGCAAAAAATGCTCTCCTTTATGATTTAAGAGTTATGGCGATGATACGGGGCTTTACCTCCCCGTAATATTGCGTTAACAGGCCGCTGACGGCTTCGAGCGTGAAg                                  <  2:7819483/140‑1 (MQ=255)

                                                                                                      cGTCCGAAGCAACAGCCACCCCACGATAACGTTTAGCAAAAAATGCTCTCCTTTATGATTTAAGAGTTATGGCGATGATACGGGGCTTTACCTCCCCGTAATATTGCGTTAACAGGCCGCTGACGGCTTCGAGCGTGAAg                                  <  2:392128/140‑1 (MQ=255)

                                                                                                        tCCGAAGCAACAGCCACCCCACGATAACGTTTAGCAAAAAATGCTCTCCTTTATGATTTAAGAGTTATGGCGATGATACGGGGCTTTACCTCCCCGTAATATTGCGTTAACAGGCCGCTGACGGCTTCGAGCGTGAAGga                                >  2:5624587/1‑140 (MQ=255)

                                                                                                        tCCGAAGCAACAGCCACCCCACGATAACGTTTAGCAAAAAATGCTCTCCTTTATGATTTAAGAGTTATGGCGATGATACGGGGCTTTACCTCCCCGTAATATTGCGTTAACAGGCCGCTGACGGCTTCGAGCGTGAAGga                                >  2:2697659/1‑140 (MQ=255)

                                                                                                        tCCGAAGCAACAGCCACCCCACGATAACGTTTAGCAAAAAATGCTCTCCTTTATGATTTAAGAGTGATGGCGATGATACGGGGCTTTACCTCCCCGTACTATTGCGTTAACAGGCCGCTGACGGCTTCGAGCGGGAAGga                                >  1:3986469/1‑140 (MQ=255)

                                                                                                           gAAGCAACAGCCACCCCACGATAACGTTTAGCAAAAAATGCTCTCCTTTATGATTTCAGAGTTATGGCGATGATACGGGGCTTTACCTCCCCGTAATATTGCGTTAACAGGCCGCTGACGGCTTCGAGCGTGAAGGAGaa                             <  2:7330891/140‑1 (MQ=255)

                                                                                                           gAAGCAACAGCCACCCCACGATAACGTTTAGCAAAAAATGCTCTCCTTTATGATTTAAGAGTTATGGCGATGATACGGGGCTTTACCTCCCCGTAATATTGCGTTAACAGGCCGCTGACGGCTTCGAGCGTGAAGGAGaa                             <  2:3550353/140‑1 (MQ=255)

                                                                                                           gAAGCAACAGCCACCCCACGATAACGTTTAGCAAAAAATGCTCTCCTTTATGATTTAAGAGTTATGGCGATGATACGGGGCTTTACCTCCCCGTAATAGTGCGTTAACAGGCCGCTGACGGCTTCGAGCGTGAAGGAGaa                             <  2:4808113/140‑1 (MQ=255)

                                                                                                             aGCAACAGCCACCCCACGATAACGTTTAGCAAAAAATGCTCTCCTTTATGATTGAAGAGTTATGGCGATGATACGGGGCTTTACCTCCCCGTAAGATTGCGTTAACAGGCCGCTGACGGCTTCGAGCGTGATGGAGAATa                           >  1:9283827/1‑140 (MQ=255)

                                                                                                              gCAACAGCCACCCCACGATAACGTTTAGCAAAAAATGCTCTCCTTTATGATTTAAGAGTTATGGCGATGATACGGGGCTTTACCTCCCCGTAATATTGCGTTAACAGGCCGCTGACGGCTTCGAGCGTGAAGGAGAATaa                          <  2:3099538/140‑1 (MQ=255)

                                                                                                              gCAACAGCCACCCCACGATAACGTTTAGCAAAAAATGCTCTCCTTTATGATTTAAGAGTTATGGCGATGATACGGGGCTTTACCTCCCCGTAATATTGCGTTAACAGGCCGCTGACGGCTTCGAGCGTGAAGGAGAATaa                          <  1:197236/140‑1 (MQ=255)

                                                                                                                aaCAGCCACCCCACGATAACGTTTAGCAAAAAATGCTCTCCTTTATGATTTAAGAGTTATGGCGATGATACGGGGCTTTACCTCCCCGTAATATTGCGTTAACAGGCCGCTGACGGCTTCGAGCGTGAAGGAGAATAACa                        <  1:6934892/140‑1 (MQ=255)

                                                                                                                 aCAGCCACCCCACGATAACGTTTAGCAAAAAATGCTCTCCTTTATGATTTAAGAGTTATGGCGATGATACGGGGCTTTACCTCCCCGTAATATTGCGTTAACAGGCCGCTGACGGCTTCGAGCGTGAAGGAGAATAACaa                       >  2:7279579/1‑140 (MQ=255)

                                                                                                                  cAGCCACCCCACGATAACGTTTAGCAAAAAATGCTCTCCTTTATGATTTAAGAGTTATGGCGATGATACGGGGCTTTACCTCCCCGTAATATTGCGTTAACAGGCCGCTGACGGCTTCGAGCGTGAAGGAGAATAACAAt                      >  1:8282768/1‑140 (MQ=255)

                                                                                                                  cAGCCACCCCACGATAACGTTTAGCAAAAAATGCTCTCCTTTATGATTTAAGAGTTATGGCGATGATACGGGGCTTTACCTCCCCGTAATATTGCGTTAACAGGCCGCTGACGGCTTCGAGCGTGAAGGAGAATAACAAt                      >  2:229271/1‑140 (MQ=255)

                                                                                                                     ccACCCCACGATAACGTTTAGCAAAAAATGCTCTCCTTTATGATTTAAGAGTTATGGCGATGATACGGGGCTTTACCTCCCCGTAATATTGCGTTAACAGGCCGCTGACGGCTTCGAGCGTGAAGGAGAATAACAATGtt                   <  1:3417966/140‑1 (MQ=255)

                                                                                                                      cACCCCACGATAACGTTTAGCAAAAAATGCTCTCCTTTATGATTTAAGAGTTATGGCGATGATACGGGGCTTTACCTCCCCGTAATATTGCGTTAACAGGCCGCTGACGGCTTCGAGCGTGAAGGAGAATAACAATGTTc                  >  2:8179414/1‑140 (MQ=255)

                                                                                                                         cccACGATAACGTATAGCAAAAAATGATCTCCTTTATGATTTAAGAGTTATGGCGATGATACGGGGCTTTACCTCCCCGTAATATTGCGTTAACAGTCCGCTGACGGCTTCGAGCGTGAAGGAGAATAACAATGTTCAgg               >  2:9010996/1‑140 (MQ=255)

                                                                                                                             cGATAACGTTTAGCAAAAAATGCTCTCCTTTATGATTTAAGAGTTATGGCGATGATACGGGGCTTTACCTCCCCGTAATATTGCGTTAACAGGCCGCTGACGGCTTCGAGCGTGAAGGAGAATAACAATGTTCAGGTCAc           >  2:6286764/1‑140 (MQ=255)

                                                                                                                                   cGTTTAGCAAAAAATGCTCTCCTTTATGATTTAAGAGTTATGGCGATGATACGGGGCTTTACCTCCCCGTAATATTGCGTTAACAGGCCGCTGACGGCTTCGAGCGTGAAGGAGAATAACAATGTTCAGGTCACTGTTTc     >  1:4769840/1‑140 (MQ=255)

                                                                                                                                   cGTTTAGCAAAAAATGCTATACTTTATGATTTAAGAGTTATTGCGATGATACGGGGCTTTACCTCCCCGTACTATTGCGTTAACAGGACGCTGACGGCTTCGAGCGTGAAGGAGAATAACAATGTTCATGTCACTGTTTc     >  2:1266237/1‑140 (MQ=255)

                                                                                                                                     tttAGCAAAAAATGCTCTCCTTTATGATTTAAGAGTTATGGCGATGATACGGGGCTTTACCTCCCCGTAATATTGCGTTAACAGGCCGCTGACGGCTTCGAGCGTGAAGGAGAATAACAATGTTCAGGTCACTGTTTCTg   >  2:4976715/1‑140 (MQ=255)

                                                                                                                                     tttAGCAAAAAATGCTCTCCTTTATGATTTAAGAGTTATGGCGATGATACGGGGCTTTACCTCCCCGTAATATTGCGTTAACAGGCCGCTGACGGCTTCGAGCGTGAAGGAGAATAACAATGTTCAGGTCACTGTTTCTg   >  1:1593555/1‑140 (MQ=255)

                                                                                                                                      ttAGCAAAAAATGCTCTCCTTTATGATTTAAGAGTTATGGCGATGATACGGGGCTTTACCTCCCCGTAATATTGCGTTAACAGGCCGCTGACGGCTTCGAGCGTGAAGGAGAATAACAATGTTCAGGTCACTGTTTCTgg  >  1:8852139/1‑140 (MQ=255)

GCGGCAGTCGTTGTACCCAATACCACATTGCTACCGCTGCTCTGTATCGTCATGCTTCCGGCATGGCTGTTATTAGCAGGTAAGCCGATGATGATGGTCAATCGTCCGAAGCAACAGCCACCCCACGATAACGTTTAACAAAAAATGCTCTCCTTTATGATTTAAGAGTTATGGCGATGATACGGGGCTTTACCTCCCCGTAATATTGCGTTAACAGGCCGCTGACGGCTTCGAGCGTGAAGGAGAATAACAATGTTCAGGTCACTGTTTCTGG  >  NZ_CP025268/2514784‑2515057

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`