BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP025268	2,529,956	T→C	309 (TAA→TAG)	yfeR ←	LysR family transcriptional regulator

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP025268	2,529,956	0	T	C	100.0%	1582.9 / NA	454	309 (TAA→TAG)	yfeR	LysR family transcriptional regulator
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (249/205); total (249/205)

Only 100 of 454 total aligned reads displayed.

AACATCACACATTACTACGGGGATTCAGTATCGATATGCGGATGACAAACTGGGCGAAGATTTTTACCAGGATGCGATAATCTACTCCATCAAATTTAACTTCTGATTGATTACTGCCGGGGCTCCCGGCACACTTTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGTACCACGTCCCATAAAGCTTCAGCGGCGGTGGAAAGTGAACGGTTCTTCCTGCGCACCAGCATCAACTGGCGTTCAA  >  NZ_CP025268/2529821‑2530092

cattactacggggattcagtatcgatatgcggatgacaaactgggcgaagatttttaccaggatgcgataatctactccatcaaatttaacttctgattgattactgccggggctcccggcagtatgaattCACTCtata                                                                                                                                      >  2:3563302‑M2/132‑140 (MQ=255)

cattactacggggattcagtatcgatatgcggatgacaaactgggcgaagatttttaccaggatgcgataatctactccatcaaatttaacttctgagtgattactgccggggctcccggcagtatgaattCACTCtata                                                                                                                                      >  1:8818881‑M2/132‑140 (MQ=255)

 cttactacggggattcagtatcgatatgcggatgacaaactgggcgaagatttttaccaggatgcgataatctactccatcaaatttaactgctgattgattactgccggggctcccggcagtatgaattCACTCTatat                                                                                                                                     <  1:2629260‑M2/10‑1 (MQ=255)

 attactacggggattcagtatcgatatgcggatgacaaactgggcgaagatttttaccaggatgcgataatctactccatcaaatttaacttctgattgattactgccggggctcccggcagtatgaattCACTCTatat                                                                                                                                     >  2:9440206‑M2/131‑140 (MQ=255)

 attactacggggattcagtatcgatatgcggatgacaaactgggcgaagatttttaccaggatgcgagaatctactccatcaaatttaacttctgattgattactgccggggctcccggcagtatgaattCACTCTatat                                                                                                                                     <  1:4895619‑M2/10‑1 (MQ=255)

  gtcctacggggattcagtatcgatatgcggatgacaaactgggcgcagatttttaccaggatgcgataatctactccatcaaatttaacttctgattgattactgccggggctcccggcagtatgaattCACTCTATATc                                                                                                                                    <  1:7509467‑M2/11‑1 (MQ=255)

     ctacggggattcagtatcgatatgcggatgacaaactgggcgaagatttttaccaggatgcgataatctactccatcaaatttaacttctgattgattactgccggggctcccggcagtatgaattCACTCTATATCTGa                                                                                                                                 <  1:1675402‑M2/14‑1 (MQ=255)

        cggggattcagtatcgatatgcggatgacaaactgggcgaagatttttaccaggatgcgataatctactccatcaaatttaacttctgattgattactgccggggctcccggcagtatgaattCACTCTATATCTGATAc                                                                                                                              >  2:6970283‑M2/124‑140 (MQ=255)

        cggggattcagtatcgatatgcggatgacaaactgggcgaagatttttaccaggatgcgataatctactccatcaaatttaacttctgattgattactgccggggctcccggcagtatgaattCACTCTATATCTGATAc                                                                                                                              <  1:8679691‑M2/17‑1 (MQ=255)

        cggggattcagtatcgatatgcggatgacaaactgggcgaagatttttaccaggatgcgataatctactccatcaaatttaacttctgattgattactgccggggctcccggcagtatgaattCACTCTATATCTGATAc                                                                                                                              >  1:1374868‑M2/124‑140 (MQ=255)

        cggggattcagtatcgatatgcggatgacaaactgggcgaagatttttaccaggatgcgataatctactccatcaaatttaacttctgattgattactgccggggctcccggcagtatgaattCACTCTATATCTGATAc                                                                                                                              <  2:3179873‑M2/17‑1 (MQ=255)

        cggggattcagtatcgatatgcggatgacaaactgggcgaagatttttaccaggatgcgataatctactccatcaaatttaacttctgattgattactgccggggctcccggcagtatgaattCACTCTATATCTGATAc                                                                                                                              >  2:4239233‑M2/124‑140 (MQ=255)

         ggggattcagtatcgatatgcggatgacaaactgggcgaagatttttaccaggatgcgataatctactccatctcatttaacttctgattgattagtgccggggctcccggcagtatgaagtCACTCTTTATCTGATACa                                                                                                                             >  2:6423768‑M2/123‑140 (MQ=255)

          gggattcagtatcgatatgcggatgacaaactgggcgaagatttttaccaggatgcgataatctactccatcaaatttaacttctgattgattactgccggggctcccggcagtatgaattCACTCTATATCTGATACaa                                                                                                                            <  1:3165229‑M2/19‑1 (MQ=255)

           ggattcagtatcgatatgcggatgacaaactgggcgaagatttttaccaggatgcgataatctactccatcaaatttaacttctgattgattactgccggggctcccggcagtatgaattCACTCTATATCTGATACAAc                                                                                                                           >  2:4017577‑M2/121‑140 (MQ=255)

             attcagtatcgatatgcggatgacaaactgggcgaagatttttaccaggatgcgataatctactccctcaaatttaccttctgattgattactgccggggctcccggcagtctgaagaCACTCTATATCTGAGACAACgg                                                                                                                         >  2:6710783‑M2/119‑140 (MQ=255)

             attcagtatcgatatgcggatgacaaactgggcgaagatttttaccaggatgcgataatctactccatcaaatttaacttctgattgattcctgccggggctcccggcagtatgaattCACTCTATATCTGATACAACgg                                                                                                                         >  1:8389281‑M2/119‑140 (MQ=255)

             attcagtatcgatatgcggatgacaaactgggcgaagatttttaccaggatgcgataatctactccatcaaatttaacttctgattgattactgccggggctcccggcagtatgacttCACTCTATATCTGATACAACgg                                                                                                                         >  1:2841466‑M2/119‑140 (MQ=255)

             attcagtatcgatatgcggatgacaaactgggcgaagatttttaccaggatgcgataatctactccatcaaatttaacttctgattgattactgccggggctcccggcagtatgaattCACTCTCTATCTGATACAACgg                                                                                                                         >  1:7754429‑M2/119‑140 (MQ=255)

             attcagtatcgatatgcggatgacaaactgggcgaagatttttaccaggatgcgataatctactccatcaaatttaacttctgattgattactgccggggctcccggcagtatgaattCACTCTATATCTGATACAACgg                                                                                                                         >  1:79432‑M2/119‑140 (MQ=255)

             attcagtatcgatatgcggatgacaaactgggcgaagatttttaccaggatgcgataatctactccatcaaatttaacttctgattgattactgccggggctcccggcagtatgaattCACTCTATATCTGATACAACgg                                                                                                                         >  1:6187006‑M2/119‑140 (MQ=255)

             attcagtatcgatatgcggatgacaaactgggcgaagatttttaccaggatgcgataatctactccatcaaatttaacttctgagtgattactgccggggctcccggcattatgaattCACTCTATATCTGATACAACgg                                                                                                                         >  1:1007948‑M2/119‑140 (MQ=255)

             attcagtatcgatatgcggaagacaaactgggcgaagatttttaccaggatgcgataatctactccatcaaatttaacttctgattgattactgccggggctcccggcagtatgaattCACTCTATATCTGATACAACgg                                                                                                                         >  1:5489414‑M2/119‑140 (MQ=255)

                cagtatcgatatgcggatgacaaactgggcgaagatttttaccaggatgcgataatctactccatcaaatttaacttctgattgattactgccggggctcccggcagtctgaattCACTCTATATCTGATACAACGGATc                                                                                                                      >  2:2209240‑M2/116‑140 (MQ=255)

                cagtatcgatatgcggatgacaaactgggcgaagattgttaccaggatgcgatcctctactccatcctatttaacgtctgattgattactgccggggctccaggcagtctgaagtCACTCTCTATCTGATACAAGggagc                                                                                                                      >  2:7857345‑M2/116‑138 (MQ=255)

                      cgatatgcggatgacaaactgggcgaagatttttaccaggatgcgataatctactccatcaaatttaacttctgattgattactgccggggctcccggcagtatgaattCACTCTATATCTGATACAACGGATCCCCTTc                                                                                                                <  1:3005701‑M2/31‑1 (MQ=255)

                       gatatgcggatgacaaactgggcgaagatttttaccaggatgcgataatctactccatcaaatttaacttctgattgattactgccggggctcccggcagtatgaattCACTCTATATCTGATACAACGGATCCCCTTcc                                                                                                               >  1:6360243‑M2/109‑140 (MQ=255)

                        atatgcggatgacaaactgggcgaagatttttaccaggatgcgataatctactccatcaaatttaacttctgattgattactgccggggctcccggcagtatgaattCACTCTATATCTGATACAACGGATCCCCTTccc                                                                                                              <  1:2467400‑M2/33‑1 (MQ=255)

                           tgcggatgacaaactgggcgaagatttttaccaggatgcgataatctactccatcaaatttaacttctgattgattactgccggggctcccggcagtatgaattCACTCTATATCTGATACAACGGATCCCCTTCCCGcc                                                                                                           <  2:4605143‑M2/36‑1 (MQ=255)

                           tgcggatgacaaactgggcgaagatttttaccaggatgcgataatctactccatcaaatttaacttctgattgattactgccggggctcccggcagtatgaattCACTCTATATCTGATACAACGGATCCCCTTCCCGcc                                                                                                           >  2:189704‑M2/105‑140 (MQ=255)

                            gcggatgacaaactgggcgaagatttttaccaggatgcgataatctactccatcaaatttaacttctgattgattactgccggggctcccggcagtatgaattCACTCTATATCTGATACAACGGATCCCCTTCCCGccc                                                                                                          <  1:335420‑M2/37‑1 (MQ=255)

                             cggatgacaaactgggcgaagatttttaccaggatgcgataatctactccatcaaatttaacttctgattgattactgccggggctcccggcagtatgaattCACTCTATATCTGATACAACGGATCCCCTTCCCGCCCa                                                                                                         <  2:2966997‑M2/38‑1 (MQ=255)

                             cggatgacaaactgggcgaagatttttaccaggatgcgataatctactccatcaaatttaacttctgattgattactgccggggctcccggcagtatgaattCACTCTATATCTGATACAACGGATCCCCTTCCCGCCCa                                                                                                         >  2:6348996‑M2/103‑140 (MQ=255)

                               gatgacaaactgggcgaagatttttaccaggatgcgataatctactccatcaaatttaacttctgattgattactgccggggctcccggcagtatgaattCACTCTATATCTGATACAACGGATCCCCTTCCCGCCCAGc                                                                                                       >  2:6041455‑M2/101‑140 (MQ=255)

                                  gacaaactgggcgaagatttttaccaggatgcgataatctactccatcaaatttaacttctgattgattactgccggggctcccggcagtatgaattCACTCTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCAt                                                                                                    >  2:5125389‑M2/98‑140 (MQ=255)

                                   acaaactgggcgaagatttttaccaggatgcgataatctactccatcaaatttaacttctgattgattactgccggggctcccggcagtctgaattCACTCTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCAtt                                                                                                   >  1:4654076‑M2/97‑140 (MQ=255)

                                    caaactgggcgaagatttttaccaggatgcgataatctactccatcaaatttaacttctgattgattactgccggggctcccggcagtatgaattCACTCTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTa                                                                                                  <  1:5420914‑M2/45‑1 (MQ=255)

                                     aaactgggcgaagatttttaccaggatgcgatactctactccatcaaatttcacttctgattgattactgccggggctcccggcagtatgaattCCCTCTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAg                                                                                                 <  2:8552355‑M2/46‑1 (MQ=255)

                                         tgggcgaagatttttaccaggatgcgataatctactccatcaaatttaacttctgattgattactgccggggctcccggcagtatgaattCACTCTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCa                                                                                             <  2:8865823‑M2/50‑1 (MQ=255)

                                         tgggcgaagatttttaccaggatgcgataatctactccatcaaatttaacttctgattgattactgccggggctcccggcagtatgaattCACTCTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCa                                                                                             <  1:2123290‑M2/50‑1 (MQ=255)

                                         tgggcgaagatttttaccaggatgcgataatctactccatcaaatttaacttctgattgattactgccggggctcccggcagtatgaattCACTCTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCa                                                                                             <  1:4114467‑M2/50‑1 (MQ=255)

                                         tgggcgaagatttttaccaggatgcgataatctactccatcaaaattaacttctgattgattactgccggggctcccggcagtatgaattCACTCTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCa                                                                                             <  1:9024456‑M2/50‑1 (MQ=255)

                                         cgggcgaagatttttaccaggatgcgataatctactccatcaaatttaacttctgattgattactgccggggctcccggcagtatgaattCACTCTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCa                                                                                             <  1:7370681‑M2/50‑1 (MQ=255)

                                          gggcgaagatttttaccaggatgcgataatctactccatcaaatttaacttctgattgattactgccggggctcccggcagtatgaattCACTCTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCAt                                                                                            <  1:6874406‑M2/51‑1 (MQ=255)

                                            gcgaagatttttaccaggatgcgataatctactccatcaaatttaacttctgattgattactgccggggctcccggcagtatgaattCACTCTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTg                                                                                          >  2:3494371‑M2/88‑140 (MQ=255)

                                            gcgaagatttttaccaggatgcgataatctactccatcaaatttaacttctgattgattactgccggggctcccggcagtatgaattCACTCTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTg                                                                                          >  1:8617218‑M2/88‑140 (MQ=255)

                                            gcgaagatttttaccaggatgcgataatctactccatcaaatttaacttctgattgattactgccggggctcccggcagtatgaattCACTCTATATCTGATACAACGGATCCCCTTCCCGCACAGCCATTAGCGCATTg                                                                                          >  2:8284682‑M2/88‑140 (MQ=255)

                                             cgaagatttttaccaggatgcgataatctactccatcaaatttaccttctgattgattactgccggggctcccggcagtatgaattCACTCTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGc                                                                                         <  1:8089530‑M2/54‑1 (MQ=255)

                                             cgaagatttttaccaggatgcgataatctactccatcaaatttaacttctgattgattactgccggggctcccggcagtatgaattCACTCTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGc                                                                                         >  2:4378551‑M2/87‑140 (MQ=255)

                                               acgatttttacccggatgcgagccgcgactccatcaaattgaacttctgattgattactgccggggctcccggcagtatgcattCACTCTATCTCAGAGACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGccc                                                                                       <  2:8049972‑M2/56‑1 (MQ=255)

                                               aagatttttaccaggatgcgataatctactccatcaaatttaacttctgattgattactgccggggctcccggcagtatgaattCACTCTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGccc                                                                                       <  1:5144652‑M2/56‑1 (MQ=255)

                                               aagatttttaccaggatgcgataatctactccatcaaatttaacgtctgattgattactgccggggctcccggcagtatgaattCACTCTCTATCTGATACAACGGATCCCCGTCCCGCCCAGCCATTAGCGCATTGccc                                                                                       <  1:5847398‑M2/56‑1 (MQ=255)

                                                 gatttttaccaggatgcgataatctactccatcaaatttaacttctgattgattactgccggggctcccggcagtatgaattCACTCTATCTCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTg                                                                                     <  2:9024728‑M2/58‑1 (MQ=255)

                                                 gatttttaccaggatgcgataatctactccatcaaatttaacttctgattgattactgccggggctcccggcagtatgaattCACTCTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTg                                                                                     <  1:9128476‑M2/58‑1 (MQ=255)

                                                      ttacccggatgcgataatctactccatcaaatttaacttctgattgattactgccggggctcccggcagtatgaattCACTCTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGtctc                                                                                <  2:5718784‑M2/63‑1 (MQ=255)

                                                           aggatgcgataatctactccatcaaatttaacttctgattgattactgccggggctcccggcagtatgaattCACTCTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGTAcc                                                                           <  1:9247248‑M2/68‑1 (MQ=255)

                                                            ggatgcgataatctactccatcaaatttaacttctgattgattactgccggggctcccggcagtatgaattCACTCTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGTACCa                                                                          <  2:7141128‑M2/69‑1 (MQ=255)

                                                              atgcgataatctactccatcaaatttaacttctgattgattactgccggggctcccggcagtatgaattCACTCTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGTACCACg                                                                        <  2:1007948‑M2/71‑1 (MQ=255)

                                                              atgcgataatctactccatcaaatttaacttctgattgattactgccggggctcccggcagtatgaattCACTCTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGTACCACg                                                                        <  2:7597559‑M2/71‑1 (MQ=255)

                                                                gcgataatctactccatcaaatttaacttctgattgattactgccggggctcccggcagtatgaattCACTCTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGTACCACGTc                                                                      >  1:1579233‑M2/68‑140 (MQ=255)

                                                                 cgataatctactccatcaaatttaacttctgattgagtactgccggggctcccggcagtatgaattCACTCTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGTACCACGTcc                                                                     <  2:3773303‑M2/74‑1 (MQ=255)

                                                                 cgataatctactccatcaaatttaacttctgatagataactgcctgggctcccggcagtatgacttCACTCTATATCTGATACAACGGATACCCTTCCCGCCCAGCCATTAGCGCATAGCCCTGGTCTCGTCCCACGTcc                                                                     >  2:5627039‑M2/67‑140 (MQ=255)

                                                                       tctactccatcaaatttaacttctgattgattactgccggggctcccggcagtatgaattCACTCTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGTACCACGTCCCATaaa                                                               >  1:1458072‑M2/61‑140 (MQ=255)

                                                                           ctccatcaaatttaacttctgattgattactgccggggctcccggcagtctgaattCACTCTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGTACCACGTCCCATAAAGCtt                                                           >  2:5312275‑M2/57‑140 (MQ=255)

                                                                           ctccatcaaatttaacttctgattgattactgccggggctcccggcagtattaattCACTCTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCCTTAGCGCATTGCCCTGTTCTCGTACCACGTCCCAGACAGCtt                                                           >  2:2050020‑M2/57‑140 (MQ=255)

                                                                           ctccatcaaatttaacttctgattgattactgccggggctcccggcagtatgaattCACTCTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGTACCACGTCCCATAAAGCtt                                                           >  2:1311491‑M2/57‑140 (MQ=255)

                                                                           ctccatcaaatttaacttctgattgattactgccggggctcccggcagtatgaattCACTCTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGTACCACGTCCCATAAAGCtt                                                           >  2:6512843‑M2/57‑140 (MQ=255)

                                                                            tccatcaaatttaacttctgattgattactgccggggctcccggcagtatgaattCACTCTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGTACCACGTCCCATAAAGCTTc                                                          >  1:8197853‑M2/56‑140 (MQ=255)

                                                                            tccatcaaatttaacttctgattgattactgccggggctcccggcagtatgaattCACTCTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCAGTAGCGCATTGCCCTGGTCTCGTACCACGTCCCATCAAGCTTc                                                          >  2:7277028‑M2/56‑140 (MQ=255)

                                                                              catcaaatttaacttctgattgattactgccggggctcccggcagtatgaattCACTCTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGTACCACGTCCCATAAAGCTTCAg                                                        <  2:3670717‑M2/87‑1 (MQ=255)

                                                                              catcaaatttaacttctgattgattactgccggggctcccggcagtatgaattCACTCTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGTACCACGTCCCATAAAGCTTCAg                                                        >  1:9483575‑M2/54‑140 (MQ=255)

                                                                              catcaaatttaacttctgattgattactgccggggctcccggcagtatgaattCACTCTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGTACCACGTCCCATAAAGCTTCAg                                                        <  1:7617178‑M2/87‑1 (MQ=255)

                                                                                 caaatttaacttctgattgattactgccggggctcccggcagtatgaattCACTCTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGTACCACGTCCCATAAAGCTTCAgcgg                                                     <  1:7022465‑M2/90‑1 (MQ=255)

                                                                                   aatttaacttctgattgattactgccggggctcccggcagtatgaattCACTCTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGTACCACGTCCCATAAAGCTTCAgcggcg                                                   >  1:4282048‑M2/49‑140 (MQ=255)

                                                                                        aacttcttattgataactgccggggctcccggcagtatgaattCACTCTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGTACCACGTCCCATAAAGCTTCAGCGGCGGTGGa                                              <  1:7332944‑M2/97‑1 (MQ=255)

                                                                                        aacttctgattgattactgccggggctcccggcagtatgaattCACTCTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGTACCACGTCCCATAAAGCTTCAGCGGCGGTGGa                                              <  1:1970710‑M2/97‑1 (MQ=255)

                                                                                        aacgtctgattgattacggccggggctcccggcagtatgaattCACTCTCTCTCTGATACAACGGAGCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGTACCACGTCCCATAAAGCTTCAGCGGCGGTGGa                                              <  2:2773091‑M2/97‑1 (MQ=255)

                                                                                             ctgattgattactgccggggctcccggcagtatgaattCACTCTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGTACCACGTCCCATAAAGCTTCAGCGGCGGTGGAAAGTg                                         >  1:4018742‑M2/39‑140 (MQ=255)

                                                                                             ctgattgattactgccggggctcccggcagtatgaattCACTCTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGTACCACGTCCCATAAAGCTTCAGCGGCGGTGGAAAGTg                                         >  1:8468928‑M2/39‑140 (MQ=255)

                                                                                              tgattgattactgccggggctcccggcagtatgaattCACTCTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGTACCACGTCCCATAAAGCTTCAGCGGCGGTGGAAAGTGa                                        <  1:1169953‑M2/103‑1 (MQ=255)

                                                                                                attgattactgccggggctcccggcagtatgaattCACTCTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGTACCACGTCCCATAAAGCTTCAGCGGCGGTGGAAAGTGAAc                                      >  1:4974820‑M2/36‑140 (MQ=255)

                                                                                                agtgatcaccgccggggctcccggcagtatgaattCACTCTATATCGGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGTACCACGTCCCATAACGCTTCAGCGGCGGTGGAAAGTGAAc                                      <  1:3911325‑M2/105‑1 (MQ=255)

                                                                                                   gattactgccggggctcccggcagtatgaattCACTCTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGTACCACGTCCCATAAAGCTTCAGCGGCGGTGGAAAGTGAACGGt                                   <  2:1367361‑M2/108‑1 (MQ=255)

                                                                                                        ctgccggggctcccggcagtatgaattCACTCTATATCTGATACAAAGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGTACCACGTCCCATAAAGCTTCAGCGGCGGTGGAAAGTGAACGGttcttc                              <  1:8344686‑M2/113‑1 (MQ=255)

                                                                                                             ggggctcccggcagtatgaattCACTCTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGTACCACGTCCCATAAAGCTTCAGCGGCGGTGGAAAGTGAACGGTTCTTCCTgcg                         <  1:6562074‑M2/118‑1 (MQ=255)

                                                                                                              gggctcccggcagtatgaattCACTCTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGTACCACGTCCCATAAAGCTTCAGCGGCGGTGGAAAGTGAACGGTTCTTCCTgcgc                        >  1:5381174‑M2/22‑140 (MQ=255)

                                                                                                              gggctcccggcagtatgaattCACTCTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGTACCACGTCCCATAAAGCTTCAGCGGCGGTGGAAAGTGAACGGTTCTTCCTgcgc                        >  1:5777535‑M2/22‑140 (MQ=255)

                                                                                                               ggctcccggcagtatgaattCACTCTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGTACCACGTCCCATAAAGCTTCAGCGGCGGTGGAAAGTGAACGGTTCTTCCTGCGCa                       >  2:543576‑M2/21‑140 (MQ=255)

                                                                                                               ggctcccggcagtatgaattCACTCTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGTACCACGTCCCATAAAGCTTCAGCGGCGGTGGAAAGTGAACGGTTCTTCCTGCGCa                       <  2:8507336‑M2/120‑1 (MQ=255)

                                                                                                                          gtatgaattCACTCTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGTACCACGTCCCATAAAGCTTCAGCGGCGGTGGAAAGTGAACGGTTCTTCCTGCGCACCAGCATCAAc            <  1:6509745‑M2/131‑1 (MQ=255)

                                                                                                                          gtatgaattCACTCTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGTACCACGTCCCATAAAGCTTCAGCGGCGGTGGAAAGTGAACGGTTCTTCCTGCGCACCAGCATCAAc            <  2:5931102‑M2/131‑1 (MQ=255)

                                                                                                                           tatgaattCACTCTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGTACCACGTCCCATAAAGCTTCAGCGGCGGTGGAAAGTGAACGGTTCTTCCTGCGCACCAGCATCAACt           >  1:2048805‑M2/9‑140 (MQ=255)

                                                                                                                           tatgaattCACTCTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGTACCACGTCCCATAAAGCTTCAGCGGCGGTGGAAAGTGAACGGTTCTTCCTGCGCACCAGCATCAACt           >  2:3402991‑M2/9‑140 (MQ=255)

                                                                                                                           tatgaattCACTCTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGTACCACGTCCCATAAAGCTTCAGCGGCGGTGGAAAGTGAACGGTTCTTCCTGCGCACCAGCATCAACt           >  1:7173205‑M2/9‑140 (MQ=255)

                                                                                                                              gaattCACTCTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGTACCACGTCCCATAAAGCTTCAGCGGCGGTGGAAAGTGAACGGTTCTTCCTGCGCACCAGCATCAACTGGc        >  1:2183895‑M2/6‑140 (MQ=255)

                                                                                                                              gaattCACTCTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGTACCACGTCCCATAAAGCTTCAGCGGCGGTGGAAAGTGAACGGTTCTTCCTGCGCACCAGCATCAACTGGc        >  1:3546202‑M2/6‑140 (MQ=255)

                                                                                                                                  tCACTCTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGTACCACGTCCCATAAAGCTTCAGCGGCGGTGGAAAGTGAACGGTTCTTCCTGCGCACCAGCATCAACTGGCGTTc    >  2:4940242‑M2/2‑140 (MQ=255)

                                                                                                                                   cacTCTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGTACCACGTCCCATAAAGCTTCAGCGGCGGTGGAAAGTGAACGGTTCTTCCTGCGCACCAGCATCAACTGGCGTTca   >  2:4435650/1‑140 (MQ=255)

                                                                                                                                   cacTCTATATCTGATACAACGGATCCCCNTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGTACCACGTCCCATAAAGCTTCAGCGGCGGTGGAAAGTGAACGGTTCTTCCTGCGCACCAGCATCAACTGGCGTTca   <  1:5568554/140‑1 (MQ=255)

                                                                                                                                   cacTCTATATCTGAGACACCGGATCCCCGTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGTACCACGTCCCATAACGCTACAGCGGCGGTGGAAAGTGAACGGTTCTTCCTGCGCACCAGCATCAACTGGCGTTca   <  1:3696969/140‑1 (MQ=255)

AACATCACACATTACTACGGGGATTCAGTATCGATATGCGGATGACAAACTGGGCGAAGATTTTTACCAGGATGCGATAATCTACTCCATCAAATTTAACTTCTGATTGATTACTGCCGGGGCTCCCGGCACACTTTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGTACCACGTCCCATAAAGCTTCAGCGGCGGTGGAAAGTGAACGGTTCTTCCTGCGCACCAGCATCAACTGGCGTTCAA  >  NZ_CP025268/2529821‑2530092

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`