BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP025268	3,174,183	T→C	111 (TAA→TAG)	ygiZ ←	DUF2645 family protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP025268	3,174,183	0	T	C	99.7%	1182.5 / ‑6.6	341	111 (TAA→TAG)	ygiZ	DUF2645 family protein
Reads supporting (aligned to +/- strand): ref base T (0/0); major base C (114/226); minor base A (0/1); total (114/227)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01

Only 100 of 342 total aligned reads displayed.

GATTGTCCAGCGAATCGCCAAATTGCATGGCATGAATGTTGAATTTGGGAATGCGGAACAAGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTATTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGACAAATACCGAGCGAGAATACATATACCCAAAACTCGAACATTTCCCGCATAAAGAGTTTTCTTAAGATAAGAATAATAAGTGGCG  >  NZ_CP025268/3174047‑3174319

   tGTCCAGCGAATCGCCAAATTGCATGGCATGAATGTTGAATTTGGGAATGCGGAACAAGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAg                                                                                                                                    >  2:7576798/1‑140 (MQ=255)

    ttCCAGCTAATCGCCAAATTGCATGGCATGAATGTTGAATTTGGGAATGCGTAAAAAGGTTGATTTGAGGCGAAGGTAAGCTGTTAAATTCTCTGTCTATGATAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGc                                                                                                                                   <  2:9082326/139‑1 (MQ=255)

    gTCCTGCTACTCGCCAAATTGCATGGCATGAATGTTGAATTTGGGATTGCGGAACATGGTGGATTTGAGGCGAAGTTAAGCTGGTAACTTCTCTGAATATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGc                                                                                                                                   <  1:1141664/140‑1 (MQ=255)

    gTCCAGCTAATCCCCAAATTGAATGGCATGAATGTTGAATTTGGGAATGCGGAACAAGGTGGATTTGAGGCGAAGTTAAGCTGTTAACTTCTATGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGc                                                                                                                                   <  2:8120427/140‑1 (MQ=255)

    gTCCAGCGAATCGCCAATTTGAATGGCATGATTTTTTAATTGGGGAATGCGGAACAAGGTGGATTTGCGGCGAAGGTAAGCTGGTAACTTCTCTGTCTTTGTGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGc                                                                                                                                   <  1:2098055/140‑1 (MQ=255)

    gTCCAGCGAATCGCCAAATTGCATGGCATGAATGTTGAATTTGGGAATGCGGAACAATGTGTATTTGAGGCGAAGGAAAGCTGGTAACTTCTCTGTCTATGAGAGCGGTTAAAACGACTCTCATAGATTTTACTAATAGc                                                                                                                                   <  1:4268434/140‑1 (MQ=255)

     tCCAGCGACTCGCCAAATTGCATGGCATGAATGTTGAATTTGGGAATGCGGAACAAGGTGGATTTGAGGCGAAGGTATGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCa                                                                                                                                  <  1:7861883/140‑1 (MQ=255)

     tCCAGCGAATCGCCAAATTGCATGTCATGAATGTTTAATTTGGGAATGCGGAACAAGGTGGATTTGAGGCTAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCa                                                                                                                                  <  1:6113789/140‑1 (MQ=255)

     tCCAGCGAATCGCCAAATTGCATGGCTTGATTGTTGAATTTGGGAATGCGGAACAAGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCa                                                                                                                                  <  1:7784136/140‑1 (MQ=255)

     tCCAGCGAATCGCCAAATTGCATGGCATGAATGTTTAATTTGGGAATGCGGAACAAGGTGGATTGGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCa                                                                                                                                  <  2:3178534/140‑1 (MQ=255)

     gccAGCGAATAGCCAAATTGCATGGCATGACTTTTGAATTTGGGAATGCGGAACAAGGTGGATTTGAGGCGAAGGTAAGATGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCa                                                                                                                                  <  2:8794910/139‑1 (MQ=255)

     cccAGCGAAGCGCCAAATTGCTTGGATTTATTTTTGATTTTGGGAATGCGGAACACGGTGGATTTGCGGCGACGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAACACGACTCTCATAGATTTTACTAATAGCa                                                                                                                                  <  1:135768/139‑1 (MQ=255)

        aGCGAATCGCCAAATTGCATGGCATGAATGTTGAATTTGGGAATGCGGAACAAGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCaaaa                                                                                                                               >  2:1855116/1‑140 (MQ=255)

          cGAATCGCCAAATTGCATGGCATGAATGTTGAATTTGGGAATGCGGAACAAGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAata                                                                                                                             >  1:2067386/1‑140 (MQ=255)

           gAATCGCCAATTTGCATGGCATGAATGTTGAATTTGGTAATGCGGAACATGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAatat                                                                                                                            <  1:2709622/140‑1 (MQ=255)

                   aaaTTGCAAGCCATGACAGTTGAATTTGGGAATGCGTAACAAGGTGGATTTGAGGCGAAGGTAAGCTGGAAACTTCTCTTTCTATGAGAGCCGTTAAAACGACACTCATAGATTTTACTAATACCAAAATATAAACCGTc                                                                                                                    <  2:5185563/140‑1 (MQ=255)

                    aaTTGCATGGCATGAATGTTGAATTTGGGAATGCGGAACAAGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTcc                                                                                                                   >  2:1159583/1‑140 (MQ=255)

                           tggcatgAATGTTGAAGTTGGGAATGCGGAACAAGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGGTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACAGACCCCaaaaa                                                                                                            >  2:8337470/1‑140 (MQ=255)

                                  aaTGTTGAATTTGGGAATGCGGAACAAGGTGGATTTGAGGCGAAGGTAAGCTGTTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGccacc                                                                                                     <  2:5560312/140‑1 (MQ=255)

                                        gAATTTGGGAATGCGGAACAAGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATATCAAAATATAAACCGTCCCCAAAAAAGCCACCAACcac                                                                                               >  1:8195700/1‑140 (MQ=255)

                                        gAATTTGGGAATGCGGAACAAGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACcac                                                                                               >  1:3735265/1‑140 (MQ=255)

                                        gAATTTGGGAATGCGGAACAAGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACcac                                                                                               >  2:4177173/1‑140 (MQ=255)

                                        gAATTTGGGAATGCGGAACAAGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACcac                                                                                               >  1:962815/1‑140 (MQ=255)

                                        gAATTTGGGAATGCGGAACAAGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACcac                                                                                               >  1:9223428/1‑140 (MQ=255)

                                        gAATTTGGGAATGCGGAACAAGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACcac                                                                                               >  2:7122533/1‑140 (MQ=255)

                                        gAATTTGGGAATGCGGAACAAGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACcac                                                                                               >  1:7054746/1‑140 (MQ=255)

                                            ttGGGATTGCGGAACAAGGTGGATTTGAGGCGAAGGTATGCTGGTAACTTCTCTGTATATGAGAGCCTTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAAc                                                                                           <  2:4915960/140‑1 (MQ=255)

                                            ttGGGAATGCGGAACAAGGTGGATTTGAGGCGATGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAAc                                                                                           <  2:4710302/140‑1 (MQ=255)

                                            ttGGGAATGCGGAACAAGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAAc                                                                                           >  1:7212847/1‑140 (MQ=255)

                                              gggAATGCGGAACAAGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCa                                                                                         >  2:9263674/1‑140 (MQ=255)

                                                      ggAACACGGTGGATTTGAGGCGATGGTAAGCTGGTAACTTCTCTGTCTATGAGCGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGACAAATa                                                                                 <  1:8814144/140‑1 (MQ=255)

                                                       gAACATGGTGGATTTGAGGCGAAGGTAAGCTGGTAAATTCTCTGACTATGAGAGCCTTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGACAAATAc                                                                                <  1:3211127/140‑1 (MQ=255)

                                                       gAACAAGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGACAAATAc                                                                                <  2:1379916/140‑1 (MQ=255)

                                                         aCAAGGTTGTTTTGAGGCGATTGTTTGGTTTTAACTTCTCTTTATATTAGTGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGACAAATACcg                                                                              <  1:1997040/140‑1 (MQ=255)

                                                           atgttGGATTTGCGGCTAAGGTAAGCTGGTAACTTATCTGTCTATGAGAGCCGTAAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGACAAATACcgag                                                                            <  1:2937717/136‑1 (MQ=255)

                                                           atggTGTATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGACAAATACcgag                                                                            <  2:680690/138‑1 (MQ=255)

                                                           atggTGGATTTGTGGCGATGTTAAGCGGGTAACTTCTCGGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGACAAATACcgag                                                                            <  1:414830/138‑1 (MQ=255)

                                                           aaGGTGGTTTTGTGGCGATGGTAAGCTGGTAACTTCTCTGTATATGTTAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGACAAATACcgag                                                                            <  1:3645582/140‑1 (MQ=255)

                                                           aaGGTGGATTTGAGGCGAAGTTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGACAAATACcgag                                                                            <  2:217142/140‑1 (MQ=255)

                                                           aaGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGACAAATACcgag                                                                            <  1:842430/140‑1 (MQ=255)

                                                           aaGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGACAAATACcgag                                                                            <  2:5307247/140‑1 (MQ=255)

                                                           aaGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTATCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGACAAATACcgag                                                                            <  1:3522095/140‑1 (MQ=255)

                                                           aaGGTGGATTGGAGGCGAAGGTAAGCTGGGAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGACAAATACcgag                                                                            <  1:7555812/140‑1 (MQ=255)

                                                            aGGTGGATTTGAGGCTAAGTTAAGCTGGTAACTTCTCTGTCTATGAGAGCCTTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGACAAATACcgagc                                                                           <  2:3875495/140‑1 (MQ=255)

                                                            aGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGACAAATACcgagc                                                                           <  1:554435/140‑1 (MQ=255)

                                                               tGGATTTGTGGCGATGGTAAGCTGTTAACTTCTCTGTCTATTAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGACAAATACcgagcgag                                                                        <  1:4941883/140‑1 (MQ=255)

                                                               tGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGACAAATACcgagcgag                                                                        >  2:4983521/1‑140 (MQ=255)

                                                                ggATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGACAAATACCGAGCgaga                                                                       <  1:4768597/140‑1 (MQ=255)

                                                                ggATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGACAAATACCGAGCgaga                                                                       <  2:2067386/140‑1 (MQ=255)

                                                                 gATTTGATGCGAAGGTAAGCTTGTAAATTCTCTGTCTATGAGTGCCTTTAAAACGAAGCTCATATATTTTAATAATAGCAAAATATAAACCTTCACCAAAAAATACACCAACCACAAACCAGACAATTACCGAGCGAGaa                                                                      <  2:2384975/140‑1 (MQ=255)

                                                                 gATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTTTGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACACACCAGACAAATACCGAGCGAGaa                                                                      <  2:3334468/140‑1 (MQ=255)

                                                                  aTTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGACAAATACCGAGCGAGAAt                                                                     >  1:4605565/1‑140 (MQ=255)

                                                                           gAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGACAAATACCGAGCGAGAATACATATAcc                                                            >  1:4372878/1‑140 (MQ=255)

                                                                           gAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGACAAATACCGAGCGAGAATACATATAcc                                                            <  2:6330672/140‑1 (MQ=255)

                                                                            aaGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCaa                                                                                                                                 >  1:8486751/1‑70 (MQ=255)

                                                                             aGGTAAGCTGGTAACTTCTCTGTCTATCCGCGCCGTAAAACCGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAACAGCCACCAACCACAAACCAGACAAATACCGAGCGAGAATACATATACCCa                                                          <  1:8972898/140‑1 (MQ=255)

                                                                                         aaCTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGACAAATACCGAGCGAGAATACATATACCCAAAACTCGAACAt                                              >  1:648085/1‑140 (MQ=255)

                                                                                             tctcTGTCTATGAGAGCCTTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGACAAATACCGAGCGAGAATACATATACCCAAAACTCGAACATTTcc                                          <  1:3316211/140‑1 (MQ=255)

                                                                                              ctctGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGACAAATACcgagcg                                                                          >  2:4247451/1‑107 (MQ=255)

                                                                                               tctGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGACAAATACCGAGCGAGAATACATATACCCAAAACTCGAACATTTCCCg                                        >  2:3509285/1‑140 (MQ=255)

                                                                                               tctGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGACAAATACCGAGCGAGAATACATATACCCAAAACTCGAACATTTCCCg                                        >  1:1350106/1‑140 (MQ=255)

                                                                                                 tGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGACAAATACCGAGCGAGAATACATATACCCAAAACTCGAACATTTCCCGCa                                      >  2:6404449/1‑140 (MQ=255)

                                                                                                  gTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGACAAATACCGAGCGAGAATACATATACCCAAAACTCGAACATTTCCCGCAt                                     >  2:7473975/1‑140 (MQ=255)

                                                                                                  gTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAAACGTCCCCAAAAAAGCCACCAACCACAAACCAGACAAATACCGAGCGAGAATACATATACCCAAAACTCGAACATTTCCCGCAt                                     <  2:2798088/140‑1 (MQ=255)

                                                                                                  gTCTATGAGAGCCGATAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGACAAATACCGAGCGAGAATACATATACCCAAAACTCGAACATTTCCCGCAt                                     <  1:4330412/140‑1 (MQ=255)

                                                                                                   tCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGACAAATACCGAGCGAGAATACATATACCCAAAACTCGAACATTTCCCGCATa                                    <  2:7850548/140‑1 (MQ=255)

                                                                                                   tCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGACAAATACCGAGCGAGAATACATATACCCAAAACTCGAACATTTCCCGCATa                                    <  2:6473840/140‑1 (MQ=255)

                                                                                                      aTGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGACAAATACCGAGCGAGAATACATATACCCAAAACTCGAACATTTcc                                          <  2:5769670/131‑1 (MQ=255)

                                                                                                      aTGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGACAAATACCGAGCGAGAATACATATACCCAAAACTCGAACATTTCCCGCATAAag                                 >  1:6446403/1‑140 (MQ=255)

                                                                                                         agagCCTTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGACAAATACCGAGCGAGAATACATATACCCAAAACTCGAACATTTCCCGCATAAAGAGt                              <  2:8527119/140‑1 (MQ=255)

                                                                                                           agCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGACAAATACCGAGCGAGAATACATATACCCAAAACTCGAACATTTCCCGCATAAAGAGttt                            >  2:2691682/1‑140 (MQ=255)

                                                                                                             ccGTTAAAACGACTCTCATAGATTTTACTAATAGCGAAATATGAACCGGCCCCTTAAAAGCCACCAACCACAAACCAGACAAAGTCCGTGCGAGAATACTTATCCCCACAGCTCGATCATTTCCCGCAGTAAGAGttttg                          >  1:5190500/1‑139 (MQ=255)

                                                                                                             ccGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAAc                                                                                           >  1:5081224/1‑75 (MQ=255)

                                                                                                             ccGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAAc                                                                                           <  2:5081224/75‑1 (MQ=255)

                                                                                                             ccGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGACAAATACCGAGCGAGaa                                                                      <  1:2248234/96‑1 (MQ=255)

                                                                                                             ccGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGACAAATACCGAGCGAGAATACATATACCCAAAACTCGAACATTTCCCGCATAAAGAGTTTTc                          >  1:7472050/1‑140 (MQ=255)

                                                                                                             ccGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGACAAATACCGAGCGAGAATACATATACCCAAAACTCGAACATTTCCCGCATAAAGAGTTTTc                          >  1:5810811/1‑140 (MQ=255)

                                                                                                             ccGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGACAAATACCGAGCGAGAATACATATACCCAAAACTCGAACATTTCCCGCATAAAGAGTTTTc                          >  1:694/1‑140 (MQ=255)

                                                                                                             ccGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGACAAATACCGAGCGAGAATACATATACCCAAAACTCGAACATTTCCCGCATAAAGAGTTTTc                          >  1:1640699/1‑140 (MQ=255)

                                                                                                             ccGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGGCCCCAAAAAAGCCACCCACCACAAACCAGACAAATACCGAGCGAGAATACAGATACCCAAAACTCGAACATTTCCCGCATAAAGAGTTTTc                          >  1:6405835/1‑140 (MQ=255)

                                                                                                               gTTACAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGACAAATACCGAGCGAGAATACATATACCCAAAACTCGAACATTTCCCGCATAAAGAGTTTTCtt                        <  2:1647126/140‑1 (MQ=255)

                                                                                                               gTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGccac                                                                                                      <  2:7187229/62‑1 (MQ=255)

                                                                                                               gTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGACAAATACCGAGCGAGAATACATATACCCAAAATTCGAACATTTCCCGCATAAAGAGTTTTCtt                        <  1:9152730/140‑1 (MQ=255)

                                                                                                               gTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGACAAATACCGAGCGAGAATACATATACCCAAAACTCGAACATTTCCCGCATAAAGAGTTTTCtt                        <  2:5731963/140‑1 (MQ=255)

                                                                                                               gTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGACAAATACCGAGCGAGAATACATATACCCAAAACTCGAACATTTCCCGCATAAAGAGTTTTCtt                        <  2:4640004/140‑1 (MQ=255)

                                                                                                                   aaaCGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGACAAATACCGAGCGAGAATACATATACCCAAAACTCGAACATTTCCCGCATAAAGAGTTTTCTtaaga                    <  1:6741226/140‑1 (MQ=255)

                                                                                                                    aaCGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGACAAATACCGAGCGAGAATACATATACCCAAAACTCGAACATTTCCCGCATAAAGAGTTTTCTtaagat                   <  2:6977505/140‑1 (MQ=255)

                                                                                                                    aaCGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGACAAATACCGAGCGAGAATACATATACCCAAAACTCGAACATTTCCCGCATAAAGAGTTTTCTtaagat                   <  2:3671388/140‑1 (MQ=255)

                                                                                                                     aCGACTCTCATAGATTTTACTAATCGCAAAATATAAACCGTCCCCAACAAAGCCACCAACCACAAACCAGACAAAGACCGCGCGCGAATACAGAGACCCAAAACTCGAACAGTTCCCGCATAAAGAGTTTTCTtaagatc                  <  2:3009106/140‑2 (MQ=255)

                                                                                                                     aCGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACCAACCAGACAAATACCGAGCGAGAATACATATACCCAAAACTCGAACATTTCCCGCATAAAGAGTTTTCTtaagata                  <  1:7134824/140‑1 (MQ=255)

                                                                                                                     aCGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGACAAATACCGAGCGAGAATACATATACCCAAAACTCGAACATTTCCCGCATAAAGAGTTTTCTtaagata                  <  2:542943/140‑1 (MQ=255)

                                                                                                                     aCGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGACAAATACCGAGCGAGAATACATATACCCAAAACTCGAACATTTCCCGCATAAAGAGTTTTCTtaagata                  <  1:2855917/140‑1 (MQ=255)

                                                                                                                     aCGACTCTCATAGATTTTACTAATAGCAAAATATAAAACGTCCCCAAAAAAGCCACCAACCACAAACAAGACAAATACAGAGAGAGAATACATATACCCAAAACTCGAACATTTCCCGCATAAAGAGTTTTATtaagata                  <  2:661593/140‑1 (MQ=255)

                                                                                                                      cGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGACAAATACCGAGCGAGAATACATATACCCAAAACTCGAACATTTCCCGCATAAAGAGTTTTCTtaagataa                 <  1:3929481/140‑1 (MQ=255)

                                                                                                                      cGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGACAAATACCGAGCGAGAATACATATACCCAAAACTCGAACATGTCCCGCATAAAGAGTTTTCTtaagataa                 <  2:8031290/140‑1 (MQ=255)

                                                                                                                        aCTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCACAAAAGCCACCAACCACAAACCAGACAAATACCGAGCGAGAATACATATACCCAAAACTCGAACATTTCCCGCATAAACAGATTTCTtaagataaga               <  2:260039/140‑1 (MQ=255)

                                                                                                                        aCTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGACAAATACCGAGCGAGAATACATATACCCAAAACTCGAACATTTCCCGCATAAAGAGTTTTCTtaagataaga               >  1:1705382/1‑140 (MQ=255)

                                                                                                                         ctctCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACCAACCTGACAAATACCGAGCGAGAATACATATACCAAAAACTCGAACATTTCACGCATAAAGAGTTTTCTtaagataagat              >  2:8957772/1‑139 (MQ=255)

                                                                                                                          tctcATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAACAGCCACCAACCCCAAACCAGACAAATACCGAGCGAGAATACATATACCCAAAACTCGAACATTTCCCGCATAAAGAGTTTTCTTAAGATAAGaat             <  2:4435469/140‑1 (MQ=255)

                                                                                                                                    tttACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGACAAATACCGAGCGAGAATACATATACCCAAAACTCGAACATTTCCCGCATAAAGAGTTTTCTTAAGATAAGAATAATAAGTGGc   >  1:618119/1‑140 (MQ=255)

GATTGTCCAGCGAATCGCCAAATTGCATGGCATGAATGTTGAATTTGGGAATGCGGAACAAGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTATTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGACAAATACCGAGCGAGAATACATATACCCAAAACTCGAACATTTCCCGCATAAAGAGTTTTCTTAAGATAAGAATAATAAGTGGCG  >  NZ_CP025268/3174047‑3174319

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`