BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP025268	3,915,245	A→G	C115R (TGT→CGT)	glmS ←	glutamine‑‑fructose‑6‑phosphate transaminase (isomerizing)

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP025268	3,915,245	0	A	G	99.8%	2980.1 / NA	857	C115R (TGT→CGT)	glmS	glutamine‑‑fructose‑6‑phosphate transaminase (isomerizing)
Reads supporting (aligned to +/- strand): ref base A (0/0); major base G (460/395); minor base T (0/2); total (460/397)
Fisher's exact test for biased strand distribution p-value = 2.14e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 858 total aligned reads displayed.

CTGTACCGTACGCACCACGCAGCTGCGGGATAGCACGCAGAACGGCCTCACGCAGAGTCCCGCCTTGTTTCAGCTCCCAGTTCACCAGATGGGCAATCACTTCGGTGTCGGTTTCAGAAACGAAGGTATAGCCACACGCTTTTAGCTCTTCACGCAGCGGTTCATGGTTTTCGATGATGCCGTTATGCACCACCACAATGTGTTCAGAAACATGCGGATGCGCATTCACTTCTGAAGGTTCACCGTGGGTCGCCCAGCGAGTGTGAGCAAT  >  NZ_CP025268/3915110‑3915380

 tGTACCGTACGCACCACTCAGCTGCGGGATAGCACGCAGAACGGCCTCACGCAGAGTCCCGCCTTGTTTCAGCTCCCAGTTCACCAGATGGGCAATCACTTCGGTGTCGGTTTCAGAAACGAAGGTATAGCCACGCGCtt                                                                                                                                    >  2:599000/1‑140 (MQ=255)

  gTACCGTACGCACCACGCAGCTGCGGGATAGCACGCAGAACGGCCTCACGCAGAGTCCCGCCTTGTTTCAGCTCCCAGTTCACCAGATGGGCAATCACTTCGGTGTCGGTTTCAGACACGAAGGTCTAGCCACGCGCttt                                                                                                                                   >  2:6375787/1‑140 (MQ=255)

   tACCGTACGCACCACGCAGCTGCGGGATAGCACGCAGAACGGCCTCACGCAGAGTCCCGCCTTGTTTCAGCTCCCAGTTCACCAGATGGGCAATCACTTCGGTGTCGGTTTCAGAAACGAAGGTATAGCCACGCGCtttt                                                                                                                                  >  1:4988790/1‑140 (MQ=255)

      cGTACGCACCACGCAGCTGCGGGATAGCACGCAGAACGGCCTCACGCAGAGTCCCGCCTTGTTTCAGCTCCCAGTTCACCAGATGGGCAATCACTTCGGTGTCGGTTTCAGAAACGAAGGTATAGCCACGCGCTTTTAGc                                                                                                                               <  2:1257591/140‑1 (MQ=255)

        tACGCACCACGCAGCTGCGGGATCGCACGCAGAACGGCCTCACGCCGAGTCCCGCCTTGTTTCAGCTCCCAGTTCACCAGATGGGCAATCACTTCGGTGTCGGTTTCAGAAACGAAGGTATAGCCGCGCGCTTTTAGctc                                                                                                                             >  2:7059046/1‑140 (MQ=255)

        tACGCACCACGCAGCTGCGGGATAGCACGCAGAACGGCCTCACGCAGAGTCCCGCCTTGTTTCAGCTCCCAGTTCACCAGATGGGCAATCACTTCGGTGTCGGTTTCAGACACGAAGGTCTAGCCACGCGCTTTTAGcgc                                                                                                                             >  2:8135781/1‑138 (MQ=255)

        tACGCACCACGCAGCTGCGGGATAGCACGCAGAACGGCCTCACGCAGAGTCCCGCCTTGTTTCAGCTCCCAGTTCACCAGATGGGCAATCACTTCGGTGTCGGTTTCAGAAACGAAGGTATAGCCACGCGCTTTTAGctc                                                                                                                             >  1:3813615/1‑140 (MQ=255)

        tACGCACCACGCAGCTGCGGGATAGCACGCAGAACGGCCTCACGCAGAGTCCCGCCTTGTTTCAGCTCCCAGTTCACCAGATGGGCAATCACTTCGGTGTCGGTTTCAGAAACGAAGGTATAGCCACGCGCTTTTAGctc                                                                                                                             >  2:2701384/1‑140 (MQ=255)

        tACGCACCACGCAGCTGCGGGATAGCACGCAGAACGGCCTCACGCAGAGTCCCGCCTTGTTTCAGCTCCCAGTTCACCAGATGGGCAATCACTTCGGTGTCGGTTTCAGAAACGAAGGTATAGCCACGCGCTTTTAGctc                                                                                                                             >  2:1056142/1‑140 (MQ=255)

        tACGCACCACGCAGCTGCGGGATAGCACGCAGAACGGCCTCACGCAGAGTCCCGCCTTGTTTCAGCTCCCAGTTCACCAGATGGGCAATCACTTCGGTGTCGGTTTCAGAAACGAAGGTATAGCCACGCGCTTTTAGctc                                                                                                                             >  1:5415672/1‑140 (MQ=255)

        tACGCACCACGCAGCTGCGGGATAGCACGCAGAACGGCCTCACGCAGAGTCCCGCCTTGTTTCAGCTCCCAGTTCACCAGATGGGCAATCACTTCGGTGTCGGTTTCAGAAACGAAGGTATAGCCACGCGCTTTTAGctc                                                                                                                             >  1:4436021/1‑140 (MQ=255)

        tACGCACCACGCAGCTGCGGGATAGCACGCAGAACGGCCTCACGCAGAGTCCCGCCTTGTTTCAGCTCCCAGTTCACCAGATGGGCAATCACTTCGGTGTCGGTTTCAGAAACGAAGGTATAGCCACGCGCTTTTAGctc                                                                                                                             >  2:6236616/1‑140 (MQ=255)

        tACGCACCACGCAGCTGCGGGATAGCACGCAGAACGGCCTCACGCAGAGTCCCGCCTTGTTTCAGCTCCCAGTTCACCAGATGGGCAATCACTTCGGTGTCGGTTTCAGAAACGAAGGTATAGCCACGCGCTTTTAGctc                                                                                                                             >  2:7222747/1‑140 (MQ=255)

        tACGCACCACGCAGCTGCGGGATAGCACGCAGAACGGCCTCACGCAGAGTCCCGCCTTGTTTCAGCTCCCAGTTCACCAGATGGGCAATCACTTCGGTGTCGGTTTCAGAAACGAAGGTATAGCCACGCGCTTTTAGctc                                                                                                                             >  2:1724522/1‑140 (MQ=255)

        tACGCACCACGCAGCTGCGGGATAGCACGCAGAACGGCCTCACGCAGAGTCCCGCCTTGTTTCAGCTCCCAGTTCACCAGATGGGCAATCACTTCGGTGTCGGTTTCAGAAACGAAGGTATAGCCACGCGCTTTTAGctc                                                                                                                             >  2:8288782/1‑140 (MQ=255)

        tACGCACCACGCAGCTGCGGGATAGCACGCAGAACGGCCTCACGCAGAGTCCCGCCTTGTTTCAGCTCCCAGTTCACCAGATGAGCAATCACTTCGGTGTCGGTTTCAGAAACGAAGGTATAGCCACGCGCTTTTAGctc                                                                                                                             >  1:4073650/1‑140 (MQ=255)

          cGCACCACGCAGCTGCGGGATAGCACGCAGAACGGCCTCACGCAGAGTCCCGCCTTGTTTCAGCTCCCAGTTCACCAGATGGGCAATCACTTCGGTGTCGGTTTCAGAAACGAAGGTATAGCCACGCGCTTTTAGCTCtt                                                                                                                           >  2:885215/1‑140 (MQ=255)

           gCACCACGCAGCTGCGGGATAGCACGCAGAACGGCCTCACGCAGAGTCCCGCCTTGTTTCAGCTCCCAGTTCACCAGATGGGCAATCACTTCGGTGTCGGTTTCAGAAACGAAGGTATAGCCACGCGCTTTTa                                                                                                                                 >  1:6996986/1‑133 (MQ=255)

           gCACCACGCAGCTGCGGGATAGCACGCAGAACGGCCTCACGCAGAGTCCCGCCTTGTTTCAGCTCCCAGTTCACCAGATGGGCAATCACTTCGGTGTCGGTTTCAGAAACGAAGGTATAGCCACGCGCTTTTAGCTCTTc                                                                                                                          >  1:2372070/1‑140 (MQ=255)

           gCACCACGCAGCTGCGGGATAGCACGCAGAACGGCCTCACGCAGAGTCCCGCCTTGTTTCAGCTCCCAGTTCACCAGATGGGCAATCACTTCGGTGTCGGTTTCAGAAACGAAGGTATAGCCACGCGCTTTTAGCTCTTc                                                                                                                          >  1:5220588/1‑140 (MQ=255)

             accacGCAGCTGCGGGATAGCACGCAGAACGGCCTCACGCAGAGTCCCGCCTTGTTTCAGCTCCCAGTTCACCAGATGGGCAATCACTTCGGTGTCGGTTTCAGTATCGAAGGTATAGCCACGCGCTTTTAGCTCTTCAc                                                                                                                        >  1:3080057/1‑140 (MQ=255)

             accacGCAGCTGCGGGATAGCACGCAGAACGGCCTCACGCAGAGTCCCGCCTTGTTTCAGCTCCCAGTTCACCAGATGGGCAATCACTTCGGTGTCGGTTTCAGAAACGAAGGTATAGCCACGCGCTTTTAGCTCTTCAc                                                                                                                        >  2:4812649/1‑140 (MQ=255)

             accacGCAGCTGCGGGATAGCACGCAGAACGGCCTCACGCAGAGTCCCGCCTTGTTTCAGCTCCCAGTTCACCAGATGGGCAATCACTTCGGTGTCGGTTTCAGAAACGAAGGTATAGCCACGCGCTTTTAGCTCTTCAc                                                                                                                        >  1:8323610/1‑140 (MQ=255)

               cacGCAGCTGCGGGATAGCACGCAGAACGGCCTCACGCAGAGTCCCGCCTTGTTTCAGCTCCCAGTTCACCAGATGGGCAATCACTTCGGTGTCGGTTTCAGAAACGAAGGTATAGCCACGCGCTTTTAGCTCTTCACGc                                                                                                                      >  2:8360981/1‑140 (MQ=255)

               cacGCAGCTGCGGGATAGCACGCAGAACGGCCTCACGCAGAGTCCCGCCTTGTTTCAGCTCCCAGTTCACCAGATGGGCAATCACTTCGGTGTCGGTTTCAGAAACGAAGGTATAGCCACGCGCTTTTAGCTCTTCACGc                                                                                                                      >  1:1632296/1‑140 (MQ=255)

                acGCAGCTGCGGGATAGCACGCAGAACGGCCTCACGCAGAGTCCCGCCTTGTTTCAGCTCCCAGTTCACCAGATGGGCAATCACTTCGGTGTCGGTTTCAGAAACGAAGGTATTGCCACGCGCTTTTAGCTCTTCACGCa                                                                                                                     >  1:8199546/1‑140 (MQ=255)

                acGCAGCTGCGGGATAGCACGCAGAACGGCCTCACGCAGAGTCCCGCCTTGTTTCAGCTCCCAGTTCACCAGATGGGCAATCACTTCGGTGTCGGTTTCAGAAACGAAGGTATAGCCACGCGCTTTTAGCTCTTCACGCa                                                                                                                     <  2:7972296/140‑1 (MQ=255)

                acGCAGCTGCGGGATAGCACGCAGAACGGCCTCACGCAGAGTCCCGCCTTGTTTCAGCTCCCAGTTCACCAGATGGGCAATCACTTCGGTGTCGGTTTCAGAAACGAAGGTATAGCCACGCGCTTTTAGCTCTTCACGCa                                                                                                                     >  2:5548612/1‑140 (MQ=255)

                acGCAGCTGCGGGATAGCACGCAGAACGGCCTCACGCAGAGTCCCGCCTTGTTTCAGCTCCCAGTTCACCAGATGGGCAATCACTTCGGTGTCGGTTTCAGAAACGAAGGTATAGCCACGCGCTTTTAGCTCTTCACGCa                                                                                                                     >  1:6336322/1‑140 (MQ=255)

                acGCAGCTGCGGGATAGCACGCAGAACGGCCTCACGCAGAGTCCCGCCTTGTTTCAGCTCCCAGTTCACCAGATGGGCAATCACTTCGGTGTCGGTTTCAGAAACGAAGGTATAGCCACGCGCATTTAGCTCTTCACGCa                                                                                                                     <  1:2752539/140‑1 (MQ=255)

                acGCAGCTGCGGGATAGCACGCAGAACGGCCTCACGCAGAGTCCCGCCTTGTTTCAGCTCCCAGTCCACCAGATGGGCAATCACTTCGGTGTCGGTTTCAGAAACGAAGGTATAGCCACGCGCTTTTAGCTCTTCTCGCa                                                                                                                     >  2:2158430/1‑140 (MQ=255)

                 cGCAGCTGCGGGATAGCACGCAGAACGGCCTCACGCAGAGTCCCGCCTTGTTTCAGCTCCCAGTTCACCAGATGGGCAATCACTTCGGTGTCGGTTTCAGAAACGAAGGTATAGCCACGCGCTTTTAGCTCTTCACGCAg                                                                                                                    <  2:4150245/140‑1 (MQ=255)

                  gCAGCTGCGGGATAGCACGCAGAACGGCCTCACGCAGAGTCCCGCCTTGTTTCAGCTCCCAGTTCACCAGATGGGCAATCACTTCGGTGTCGGTTTCAGAAACGAAGGTATAGCCACGCGCTTTTAGCTCTTCACGCAGc                                                                                                                   >  1:9526300/1‑140 (MQ=255)

                       tGCGGGATAGCACGCAGAACGGCCTCACGCAGAGTCCCGCCTTGTTTCAGCTCCCAGTTCACCAGATGGGCAATCACTTCGGTGTCGGTTTCAGAAACGAAGGTATAGCCACGCGCTTTTAGCTCTTCACGCAGCGGTTc                                                                                                              >  1:4722624/1‑140 (MQ=255)

                         cGGGATAGCACGCAGAACGGCCTCACGCAGAGTCCCGCCTTGTTTCAGCTCCCAGTTCACCAGATGGGCCATCACTTCGGTGTCGGTTTCAGAAACGAAGGTATAGCCACGCGCTTTTAGCTCTTCACGCAGCGGGTCAt                                                                                                            >  2:5736102/1‑140 (MQ=255)

                         cGGGATAGCACGCAGAACGGCCTCACGCAGAGTCCCGCCTTGTTTCAGCTCCCAGTTCACCAGATGGGCAATCACTTCGGTGTCGGTTTCAGAAACGAAGGTATAGCCACGCGCTTTTAGCTCTTCACGCAGCGGTTCAt                                                                                                            >  1:7655162/1‑140 (MQ=255)

                                gCACGCAGAACGGCCTCACGCAGAGTCCCGCCTTGTTTCAGCTCCCAGTTCACCAGATGGGCAATCACTTCGGTGTCGGTTTCAGAAACGAAGGTATAGCCACGCGCTTTTAGCTCTTCACGCAGCGGTTCATGGTTTTc                                                                                                     >  1:7346876/1‑140 (MQ=255)

                                   cGCAGAACGGCCTCACGCAGAGTCCCGCCTTGTTTCAGCTCCCAGTTCACCAGATGGGCAATCACTTCGGTGTCGGTTTCAGAAACGAAGGTATAGCCACGCGCTTTTAGCTCTCCACGCAGCGGTTCATGGTTTTCgat                                                                                                  >  2:8714395/1‑140 (MQ=255)

                                     cAGAACGGCCTCACGCAGAGTCCCGCCTTGTTTCAGCTCCCAGTTCACCAGATGGGCAATCACTTCGGTGTCGGTTTCAGAAACGAAGGTATAGCCACGCGCTTTTAGCTCTTCACGCAGCGGTTCATGGTTTTCgatga                                                                                                <  1:947469/140‑1 (MQ=255)

                                       gAACGGCCTCACGCAGAGTCCCGCCTTGTTTCAGCTCCCAGTTCACCAGATGGGCAATCACTTCGGTGTCGGTTTCAGAAACGAAGGTATAGCCACGCGCTTTTAGCTCTTCACGCAGCGGTTCATGGTTTTCGatgatg                                                                                              <  1:7524040/140‑1 (MQ=255)

                                        aaCGGCCTCACGCAGAGTCCCGCCTTGTTTCAGCTCCCAGTTCACCAGATGGGCAATCACTTCGGTGTCGGTTTCAGAAACGAAGGTATAGCCACGCGCTTTTAGCTCTTCACGCAGCGGTGCATGGTTTTCGATGATGc                                                                                             >  1:601714/1‑140 (MQ=255)

                                          cgaccTCACGCAGAGTCCCGCCTTGTTTCAGCTCCCAGTTCACCAGATGGGCAATCACTTCGGTGTCGGTTTCAGAAACGAAGGTATAGCCACGCGCTTTTAGCTCTTCACGCAGCGGTTCATGGTTTTCGATGATGCCg                                                                                           <  1:4868381/137‑1 (MQ=255)

                                          cGGCCTCACGCAGAGTCCCGCCTTGTGTCAGCTCCCAGTTCACCAGATGGGCAATCACTTCGGTGTCGGTTTCAGAAACGAAGGTATAGCCACGCGCTTTTAGCTCTTCACGCAGCGGTTCATGGTTTTCGATGATGCCg                                                                                           <  2:9259370/140‑1 (MQ=255)

                                           ggCCTCACGCAGAGTCCCGCCTTGTTTCAGCTCCCAGTTCACCCGATGGGCAATCACTTCGGTGTCGGTTTCAGAAACGCAGGGATAGCCACGCGCTTTTAGCTCTTCACGCAGCGGTTCATGGTTTTCGTTGATGCCgg                                                                                          >  2:3352376/1‑139 (MQ=255)

                                             ccTCACGCAGAGTCCCGCCTTGTTTCAGCTCCCAGTTCACCAGATGGTCAATCACTTCGGTGTCGGTTTCAGAAACGAAGGTATAGCCACGCGCTTTTAGCTCTTCACGCAGCGGTTCATGGTTTTCGATGATGCCGTTa                                                                                        <  2:6709134/140‑1 (MQ=255)

                                             ccTCACGCAGAGTCCCGCCTTGTTTCAGCTCCCAGTTCACCAGATGGGCAATCACTTCGGTGTCGGTTTCAGAAACGAAGGTATAGCCACGCGCTTTTAGCTCTTCACGCAGCGGTTCATGGTTTTCGATGATGCCGTTa                                                                                        <  2:423184/140‑1 (MQ=255)

                                             ccTCACGCAGAGTCCCGCCTTGTTTCAGCTCCCAGTTCACCAGATGGGCAATCACTTCGGTGTCGGTTTCAGAAACGAAGGTATAGCCACGCGCTTTTAGCTCTTCACGCAGCGGTTCATGGTTTTCGATGATGCCGTTa                                                                                        <  1:2592264/140‑1 (MQ=255)

                                                 aCGCAGAGTCCCGCCTTGTTTCAGCTCCCAGTTCACCAGATGGGCAATCACTTCGGTGTCGGTTTCAGAAACGAAGGTATAGCCACGCGCTTTTAGCTCTTCACGCAGCGGTTCATGGTTTTCGATGATGCCGTTATGca                                                                                    <  1:1235005/140‑1 (MQ=255)

                                                 aCGCAGAGTCCCGCCTTGTTTCAGCTCCCAGTTCACCAGATGGGCAATCACTTCGGTGTCGGTTTCAGAAACGAAGGTATAGCCACGCGCTTTTAGCTCTTCACGCAGCGGTTCATGGTTTTCGATGATGCCGTTATGca                                                                                    <  1:9006626/140‑1 (MQ=255)

                                                 aCGCAGAGTCCCGCCTTGTTTCAGCTCCCAGTTCACCAGATGGGCAATCACTTCGGTGTCGGTTTCAGAAACGAAGGTATAGCCACGCGCTTTTAGCTCTTCACGCAGCGGTTCATGGTTTTCGATGATGCCGTTATGca                                                                                    <  1:7208516/140‑1 (MQ=255)

                                                  cGCAGAGTCCCGCCTTGTTTCAGCTCCCAGTTCACCAGATGGGCAATCACTTCGGTGTCGGTTTCAGAAACGAAGGTATAGCCACGCGCTTTTAGCTCTTCACGCAGCGGTTCATGGTTTTCGATGATGCCGTTATGcac                                                                                   >  2:4402092/1‑140 (MQ=255)

                                                  cGCAGAGTCCCGCCTTGTTTCAGCTCCCAGTTCACCAGATGGCCAATCACATCGGTGTCGGTTTCAGAAACGAAGGTATAGCCACGCGATTATAGCTCTGCTCGCAGCGGTTCAAGGTTTTCGAGGCAGCCGTTATGcac                                                                                   >  2:5260377/1‑140 (MQ=255)

                                                     agagTCCCGCCTTGTTTCAGCTCCCAGGTCACCAGATGGGCAATCACTTCGGTGTCGGTTTCAGAAACGAAGGTATAGCCACGCGCTTTTAGCTCTTCACGCAGCGGTTCATGGTTTTCGATGATGCCGTTATGcaccac                                                                                <  2:382973/140‑1 (MQ=255)

                                                      gagTCCCGCCTTGTTTCAGCTCCCAGTTCACCAGATGGGCAATCACTTCGGTGTCGGTTTCAGAAACGAAGGTATAGCCACGCGCTTTTAGCTCTTCACGCAGCGGTTCATGGTTTTCGATGATGCCGTTATGcaccacc                                                                               <  2:8560/140‑1 (MQ=255)

                                                      gagTCCCGCCTTGTTTCAGCTCCCAGTTCACCAGATGGGCAATCACTTCGGTGTCGGTTTCAGAAACGAAGGTATAGCCACGCGCTTTTAGCTCTTCACGCAGCGGTTCATGGTTTTCGATGATGCCGTTATGcaccacc                                                                               <  2:5075940/140‑1 (MQ=255)

                                                      gagTCCCGCCTTGTTTCAGCTCCCAGTTCACCAGATGGGCAATCACTTCGGTGTCGGTTTCAGAAACGAAGGTATAGCCACGCGCTTTTAGCTCTTCACGCAGCGGTTCATGGTTTTCGATGATGCCGTTATGcaccacc                                                                               <  2:2321209/140‑1 (MQ=255)

                                                       agTCCCGCCTTGTTTCAGCTCCCAGTTCACCAGATGGGCAATCACTTCGGTGTCGGTTTCAGAAACGAAGGTATAGCCACGCGCTTTTAGCTCTTCACGCAGCGGTTCATGGTTTTCGATGATGCCGTTATGcaccacca                                                                              >  2:7723555/1‑140 (MQ=255)

                                                         tCCCGCCTTGTTTCAGCTCCCAGTTCACCAGATGGGCAATCACTTCGGTGTCGGTTTCAGAAACGAAGGTATAGCCACGCGNTTTTAGCTCTTCACGCAGCGGTTCATGGTTTTCGATGATGCCGTTATGCACCACcaca                                                                            <  1:5592849/140‑1 (MQ=255)

                                                         tCCCGCCTTGTTTCAGCTCCCAGTTCACCAGATGGGCAATCACTTCGGTGTCGGTTTCAGAAACGAAGGTATAGCCACGCGCTTTTAGCTCTTCACGCAGCGGTTCATGGTTTTCGATGATGCCGTTATGCACCACcaca                                                                            >  2:187219/1‑140 (MQ=255)

                                                         tCCCGCCTTGTTTCAGCTCCCAGTTCACCAGATGGGCAATCACTTCGGTGTCGGTTTCAGAAACGAAGGTATAGCCACGCGCTTTTAGCTCTTCACGCAGCGGTTCATGGTTTTCGATGATGCCGTTATGCACCACcaca                                                                            <  1:3527824/140‑1 (MQ=255)

                                                         tCCCGCCTTGTTTCAGCTCCCAGTTCACCAGATGGGCAATCACTTCGGTGTCGGTTTCAGAAACGAAGGTATAGCCACGCGCTTTTAGCTCTTCACGCAGCGGTTCATGGTTTTCGATGATGCCGTTATGCACCACcaca                                                                            <  1:7830149/140‑1 (MQ=255)

                                                         tCCCGCCTTGTTTCAGCTCCCAGTTCACCAGATGGGCAATCACTTCGGTGTCGGTTTCAGAAACGAAGGTATAGCCACGCGCTTTTAGCTCTTCACGCAGCGGTTCATGGTTTTCGATGATGCCGTTATGCACCACcaca                                                                            <  1:2155012/140‑1 (MQ=255)

                                                         tCCCGCCTTGTTTCAGCTCCCAGTTCACCAGATGGGCAATCACTTCGGTGTCGGTTTCAGAAACGAAGGTATAGCCACGCGCTTTTAGCTCTTCACGCAGCGGTTCATGGTGTTCGATGATGCCGTTATGCACCACcaca                                                                            >  2:7580002/1‑140 (MQ=255)

                                                           ccGCCTTGTTTCAGCTCCCAGTTCACCAGATGGGCACTCACTTCGGTGTCGGTTTCAGAAACGAAGGTATAGCCACGCGCTTTTAGCTCTTCACGCAGCGGTTCATGGTTTTCGATGACGCCGTTATGCACCACCACAAt                                                                          >  2:5427835/1‑140 (MQ=255)

                                                           ccGCCTCGTTCCAGCTCCCAGTTCCCCAGATGGGCAATCACTGCGGTGTCGGTTACAGAAACGAAGGTATAGCCACGCGCTTTTAGCTCTTCACGCAGCGGTTCATGGTTGTCGATGATGCCGTTATGCACCACCACAAt                                                                          <  2:9455837/140‑1 (MQ=255)

                                                                   tttCAGCTCCCAGTTCACCAGATGGGCAATCACTTCGGTGTCGGTTTCAGAAACGAAGGTATAGCCACGCGCTTTTAGCTCTTCACGCAGCGGTTCATGGTTTTCGATGATGCCGTTATGCACCTCCACAATGTGTTCAg                                                                  >  2:6145971/1‑140 (MQ=255)

                                                                   tttCAGCTCCCAGTTCACCAGATGGGCAATCACTTCGGTGTCGGTTTCAGAAACGAAGGTATAGCCACGCGCTTTTAGCTCTTCACGCAGCGGTTCATGGTTTTCGATGATGCCGTTATGCACCACCACAATGTGTTCAg                                                                  >  2:6848940/1‑140 (MQ=255)

                                                                   tttCAGCTCCCAGTTCACCAGATGGGCAATCACTTCGGTGTCGGTTTCAGAAACGAAGGTATAGCCACGCGCTTTTAGCTCTTCACGCAGCGGTTCATGGTTTTCGATGATGCCGTTATGCACCACCACAATGTGTTCAg                                                                  >  2:4640053/1‑140 (MQ=255)

                                                                   tttCAGCTCCCAGTTCACCAGATGGGCAATCACTTCGGTGTCGGTTTCAGAAACGAAGGTATAGCCACGCGCTTTTAGCTCTTCACGCAGCGGTTCATGGTTTTCGATGATGCCGTTATGCACCACCACAATGTGTTCAg                                                                  >  1:3424569/1‑140 (MQ=255)

                                                                             cAGTTCACCAGATGGGCAATCACTTCGGTGTCGGTTTCAGAAACGAAGGTATAGCCACGCGCTTTTATCTCTTCACGCAGCGGTTCATGGTTTTCGATGATGCCGTTATGCACCACCACAATGTGTTCAGAAACATGCgg                                                        >  1:226982/1‑140 (MQ=255)

                                                                             cAGTTCACCAGATGGGCAATCACTTCGGTGTCGGTTTCAGAAACGAAGGTATAGCCACGCGCTTTTAGCTCTTCAAGCCGCGGTTCATGGTTTTCGATGATGCAGTTATGCACCACCACAATGTGTTCAGAAACATGCgg                                                        >  2:9041617/1‑140 (MQ=255)

                                                                              aGTTCACCAGATGGGCAATCACTTCGGTGTCGGTTTCAGAAACGAAGGTATAGCCACGCGCTTTTAGCTCTTCACGCAGCGGTTCATGGTTTTCGATGATGCCGTTATGCACCACCACAATGTGTTCAGAAACATGCggc                                                       >  1:1470635/1‑139 (MQ=255)

                                                                                ttCACCAGATGGGCAATCACTTCGGTGTCGGTTTCAGAAACGAAGGTATAGCCACGCGCTTTTAGCTCTTCACGCAGCGGTTCATGGTTTTCGATGATGCCGTTATGCACCACCACAATGTGTTCAGAAACATGCGGATg                                                     <  1:6625543/140‑1 (MQ=255)

                                                                                   aCCAGATGGGCAATCACTTCGGTGTCGGTTTCAGAAACGAAGGTATAGCCACGCGCTTTTAGCTCTTCACGCAGCGGTTCATGGTTTTCGATGATGCCGTTATGCACCACCACAATGTGTTCAGAAACATGCGGATgcgc                                                  <  1:6747304/140‑1 (MQ=255)

                                                                                      aGATGGGCAATCACTTCGGTGTCGGTTTCAGAAACGAAGGTATAGCCACGCGCTTTTAGCTCTTCACGCAGCGGTTCATGGTTTTCGATGATGCCGTTATGCACCACCACAATGTGTTCAGAAACATGCGGATGCGCAtt                                               <  1:287492/140‑1 (MQ=255)

                                                                                        aTGGGCAATCACTTCGGTGTCGGTTTCAGAAACGAAGGTATAGCCACGCGCTTTTAGCTCTTCACGCAGCGGTTCATGGTTTTCGATGATGCCGTTATGCACCACCACAATGTGTTCAGAAACATGCGGATGCGCATTCa                                             <  2:303296/140‑1 (MQ=255)

                                                                                              aaTCACTTCGGTGTCGGTTTCAGAAACGAAGGTATAGCCACGCGCTTTTAGCTCTTCACGCAGCGGTTCATGGTTTTCGATGATGCCGTTATGCACCACCACAATGTGTTCAGAAACATGCGGATGCGCATTCACTTCTg                                       <  1:5729805/140‑1 (MQ=255)

                                                                                              aaTCACTTCGGTGTCGGTTTCAGAAACGAAGGTATAGCCACGCGCTTTTAGCTCTTCACGCAGCGGTTCATGGTTTTCGATGATGCCGTTATGCACCACCACAATGTGTTCAGAAACATGCGGATGCGCATTCACTTCTg                                       <  2:2531145/140‑1 (MQ=255)

                                                                                               aTCACTTCGGTGTCGGATTCAGAAACGAAGGTATAGCCACGCGCTTTTAGCTCTTCACGCAGCGGTTCATGGTTTTCGATGATGCCGTTATGCACCACCCCAATGTGTTCAGAAACATGCGGATGCGCATTCACTTCTGa                                      <  2:656595/140‑1 (MQ=255)

                                                                                                  aCTTCGGTGTCGGTTTCAGAAACGAAGGTATAGCCACGCGCTTTTAGCGCTTCTCGCAGCGGTTCATGGTGTTCGAGGTTGCCGTTATGCACCACCCCAATGTGTGCAGCAACATGCGGTGGCGCATTCACTGCTGAAgg                                   >  2:3640776/1‑140 (MQ=255)

                                                                                                   cTTCGGTGTCGGTTTCAGAAACGAAGGTATAGCCACGCGCTTTTAGCTCTTCACGCAGCGGTTCATGGTTTTCGATGATGCCGTTATGCACCACCACAATGTGTTCAGAAACATGCGGATGCGCATTCACTTCTGAAGGt                                  <  1:7957995/140‑1 (MQ=255)

                                                                                                   cTTCGGTGTCGGTTTCAGAAACGAAGGTATAGCCACGCGCTTTTAGCTCTTCACGCAGCGGTTCATGGTTTTCGATGATGCCGTTATGCACCACCACAATGTGTTCAGAAACATGCGGATGCGCATTCACTTCTGAAGGt                                  <  1:1021202/140‑1 (MQ=255)

                                                                                                   cTTCGGGGTCGGTGTCAGAAACGAAGGTATAGCCACGCGCTTTTAGCTCTTCACGCAGCGGTTCATGGTTTTCGATGATGCCGTTATGCACCACCACAATGTGTTCAGAAACATGCGGATGCGCATTCACTTCTGAAggg                                  <  1:9335564/140‑2 (MQ=255)

                                                                                                      cGGTGTCGGTTTCAGAAACGAAGGTATAGCCACGCGCTTTTAGCTCTTCACGCAGCGGTTCATGGTTTTCGATGATGCCGTTATGCACCACCACAATGTGTTCAGAAACATGCGGATGCGCATTCACTTCTGAAGGTTCa                               >  1:135234/1‑140 (MQ=255)

                                                                                                       ggTGTCGGTTTCAGAAACGAAGGTATAGCCACGCGCTTTTAGCTCTTCACGCAGCGGTTCATGGTTTTCGATGATGCCGTTATGCACCACCACAATGTGTTCAGAAACATGCGGATGCGCATTCACTTCTGAAGGTTCAc                              >  2:4007231/1‑140 (MQ=255)

                                                                                                              gTTTCAGAAACGAAGGTATAGCCACGCGCTTTTAGCTCTTCACGCAGCGGTTCATGGTTTTCGATGATGCCGTTATGCACCACCACAATGTGTTCAGAAACATTCGGATGCGCATTCACTTATGAAGGTTCACCGTGGGt                       >  2:5866695/1‑140 (MQ=255)

                                                                                                                     aaaCGAAGGTATAGCCACGCGCTTTTAGCTCTTCACGCAGCGGTTCATGGTTTTCGATGATGCCGTTATGCACCACCACAATGTGTTCAGAAACATGCGGATGCGCATTCACTTCTGAAGGTTCACCGTGGGTCGCCCAg                <  1:330042/140‑1 (MQ=255)

                                                                                                                     aaaCGAAGGTATAGCCACGCGCCTTGAGCTCTTCACGCAGCGGTTCATGGTTTTCGATGAAGCCGTTATGCACCACCACAATGTGTTCAGAAACATGCGGATGCGCATTCACTTCTGAAGGTTCACCGTGGGTCGCCCAg                <  1:1995768/140‑1 (MQ=255)

                                                                                                                      aaCGAAGGTATAGCCACGCGCTTTTAGCTCTTCACGCAGCGGTTCATGGTTTTCGATGATGCCGTTATGCACCACCACACTGTGTTCAGAAACATGCGGATGCGCATTCACTTCTGAAGGTTCACCGTGGGTCGCCCAGc               <  1:9167195/140‑1 (MQ=255)

                                                                                                                          aaGGTATAGCCACGCGCTTTTAGCTCTTCACGCAGCGGTTCATGGTTTTCGATGATGCCGTTATGCACCACCACAATGTGTTCAGAAACATGCGGATGCGCATTCACTTCTGAAGGTTCACCGTGGGTCGCCCAGCGAgt           <  1:1847559/140‑1 (MQ=255)

                                                                                                                          aaGGTATAGCCACGCGCTTTTAGCTCTTCACGCAGCGGTTCATGGTTTTCGATGATGCCGTTATGCACCACCACAATGTGTTCAGAAACATGCGGATGCGCATTCACTTCTGAAGGTTCACCGTGGGTCGCCCAGCGAgt           <  1:6126372/140‑1 (MQ=255)

                                                                                                                           cggTATAGCCACGCGCTTTTAGCTCGTCACGCAGCGGTTCATGGTTTTCGAGGATGCCGTTATGCACCACCACACTGTGTTCAGAACCATGCGGATGCGCATTCACTTCGGAAGGTTCACCGTGGGTCGCCCAGCGAgtg          <  1:3633316/139‑1 (MQ=255)

                                                                                                                           aGGTAGAGCCACGCGCTTTTAGCTCTTCACGCAGCGGTTCATGGTTTTCGATGATGCCGTTATGCACCACCACAATGTGTTCAGAAACATGCGGATGCGCATTCACTTCTGAAGGTTCACCGTGGGTCGCCCAGCGAgtg          <  1:8673567/140‑1 (MQ=255)

                                                                                                                             gTATAGCCACGCGCTTTTAGCTCTTCACGCAGCGGTTCATGGTTTTCGATGATGCCGTTATGCACCACCACAATGTGTTCAGAAACATGCGGATGCGCATTCACTTCTGAAGGTTCACCGTGGGTCGCCCAGCGAGtgtg        <  1:5321676/140‑1 (MQ=255)

                                                                                                                             gTATAGCCACGCGCTTTGAGCTATTCACTCAGCGGTTCATGTTTTTCGATGATGCCGTTATGCACCACCACAATGTGTTCTGAAACATGCGGATGCGCATTCACTTCTGAAGGTTCACCGTTGGTCGCCCAGCGAGtgtg        <  2:3546274/140‑1 (MQ=255)

                                                                                                                               ataGCCACGCGCTTTTAGCTCTTCACGCAGCGGTTCATGGTTTTCGATGATGCCGTTATGCACCACCACAATGTGTTCAGACACATGCGGATGCGCATTCACTTCTGAAGGTTCACCGTGGGTCGCCCAGCGAGTGTGAg      <  1:6416682/140‑1 (MQ=255)

                                                                                                                               ataGCCACGCGCTTTTAGCTCTTCACGCAGCGGTTCATGGTTTTCGATGATGCCGTTATGCACCACCACAATGTGTTCAGAAACATGCGGATGCGCATTCACTTCTGAAGGTTCACCGTGGGTCGCCCAGCGAGTGTGAg      <  2:1424159/140‑1 (MQ=255)

                                                                                                                               ataGCCACGCGCTTTTAGCTCTTCACGCAGCGGTTCATGGTTTTCGATGATGCCGTTATGCACCACCACAATGTGTTCAGAAACATGCGGATGCGCATTCACTTCTGAAGGTTCACCGTGGGTCGCCCAGCGAGTGTGAg      <  1:3993390/140‑1 (MQ=255)

                                                                                                                               ataGCCACGCGCTTTTAGCTCTTCACGCAGCGGTTCATGGTTTTCGATGATGCCGTTATGCACCACCACAATGTGTTCAGAAACATGCGGATGCGCATTCACTTCTGAAGGTTCACCGTGGGTCGCCCAGCGAGTGTGAg      <  1:4253846/140‑1 (MQ=255)

                                                                                                                               ataGCCACGCGCTTTTAGCTCTTCACGCAGCGGTTCATGGTTTTCGATGATGCCGTTATGCACCACCACAATGTGTTCAGAAACATGCGGATGCGCATTCACTTCTGAAGGTTCACCGTGGGTCGCCCAGCGAGTGTGAg      <  2:2904227/140‑1 (MQ=255)

CTGTACCGTACGCACCACGCAGCTGCGGGATAGCACGCAGAACGGCCTCACGCAGAGTCCCGCCTTGTTTCAGCTCCCAGTTCACCAGATGGGCAATCACTTCGGTGTCGGTTTCAGAAACGAAGGTATAGCCACACGCTTTTAGCTCTTCACGCAGCGGTTCATGGTTTTCGATGATGCCGTTATGCACCACCACAATGTGTTCAGAAACATGCGGATGCGCATTCACTTCTGAAGGTTCACCGTGGGTCGCCCAGCGAGTGTGAGCAAT  >  NZ_CP025268/3915110‑3915380

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`