Predicted mutation
evidence seq id position mutation annotation gene description
RA NZ_CP025268 3,941,138 A→G *331* (TAA→TAG) 
F465S (TTT→TCT) 		 rbsR →
mdtD ←		 ribose operon transcriptional repressor RbsR
multidrug transporter subunit MdtD

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0252683,941,1380AG99.4% 2276.7 / NA 653*331* (TAA→TAG) 
F465S (TTT→TCT) 		rbsR
mdtD		ribose operon transcriptional repressor RbsR
multidrug transporter subunit MdtD
Reads supporting (aligned to +/- strand):  ref base A (0/0);  major base G (349/300);  minor base T (3/1);  total (352/301)
Fisher's exact test for biased strand distribution p-value = 6.28e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 653 total aligned reads displayed.

TAACCACTATCCACCAACCGAAAGATGAACTGGGGGAGCTGGCGATTGATGTACTCATCCATCGGATAACCCAGCCGACCCTTCAGCAACAACGATTACAACTTACTCCGATTCTGATGGAACGCGGTTCGGCTTAAATTTACGCTGTCTTTTGATCAAATTATTACCATCGGTTGTTTTCAGAAGCATGAACATTGCTGCTGAAGCAACAGTAATAATGCCCATTGTGATAAACGTATAGTGGAATTGTTCGACAGTCGTTGTGCCTTCCAT  >  NZ_CP025268/3941002‑3941274
                                                                                                                                        |                                                                                                                                        
    cACTATCCACCAACCGAAAGATGAACTGGGGGAGCTGGCGATTGATGTACTCATCCATCGGATAACCCAGCCGACCCTTCAGCAACAACGATTACAACTTACTCCGATTCTGATGGAACGCGGTTCGGCTTAGATTTACg                                                                                                                                   <  2:8814094/140‑1 (MQ=255)
    cACTATCCACCAACCGAAAGATGAACTGGGGGAGCTGGCGATTGATGTACTCATCCATCGGATAACCCAGCCGACCCTTCAGCAACAACGATTACAACTTACTCCGATTCTGATGGAACGCGGTTCGGCTTAGATTTACg                                                                                                                                   <  1:1392584/140‑1 (MQ=255)
    cACTATCCACCAACCGAAAGATGAACTGGGGGAGCTGGCGATTGATGTACTCATCCATCGGATAACCCAGCCGACCCTTCAGCAACAACGATTACAACTTACTCCGATTCTGATGGAACGCGGTTCGGCTTAGATTTACg                                                                                                                                   <  2:4900336/140‑1 (MQ=255)
     aCTATCCACCAACCGAAAGATGAACTGGGGGAGCTGGCGATTGATGTACTCATCCATCGGATAACCCAGCCGACCCTTCAGCAACAACGATTACAACTTACTCCGATTCTGATGGAACGCGGTTCGGCTTAGATTTACGc                                                                                                                                  <  2:5292812/140‑1 (MQ=255)
      cTATCCACCAACCGAAAGATGAACTGGGGGAGCTGGCGATTGATGTACTCATCCATCGGATAACCCAGCCGACCCTTCAGCAACAACGATTACAACTTACTCCGATTCTGATGGAACGCGGTTCGGCTTAGATTTACGCt                                                                                                                                 >  1:7652420/1‑140 (MQ=255)
      cTATCCACCAACCGAAAGATGAACTGGGGGAGCTGGCGATTGATGTACTCATCCATCGGATAACCCAGCCGACCCTTCAGCAACAACGATTACAACTTACTCCGATTCTGATGGAACGCGGTTCGGCTTAGATTTACGCt                                                                                                                                 <  2:5688586/140‑1 (MQ=255)
      cTATCCACCAACCGAAAGATGAACTGGGGGAGCTGGCGATTGATGTACTCATCCATCGGATAACCCAGCCGACCCTTCAGCAACAACGATTACAACTTACTCCGATTCTGATGGAACGCGGTTCGGCTTAGATTTACGCt                                                                                                                                 >  2:908319/1‑140 (MQ=255)
       tATCCACCAACCGAAAGATGAACTGGGGGAGCTGGCGATTGATGTACTCATCCATCGGATAACCCAGCCGACCCTTCAGCAACAACGATTACAACTTACTCCGATTCTGATGGAACGCGGTTCGGCTTAGATGTACGCTg                                                                                                                                >  1:5977454/1‑140 (MQ=255)
         tCCACCAACCGAAAGATGAACTGGGGGAGCTGGCGATTGATGTACTCATCCATCGGATAACCCAGCCGACCCTTCAGCAACAACGATTACCACTTACTCCGATTCTGATGGAACGCGGTTCGGCTTAGATTTACGCTGTc                                                                                                                              >  2:9195821/1‑140 (MQ=255)
         tCCACCAACCGAAAGATGAACTGGGGGAGCTGGCGATTGATGTACTCATCCATCGGATAACCCAGCCGACCCTTCAGCAACAACGATTACAACTTACTCCGATTCTGATGGAACGCGGTTCGGCTTAGATTTACGCTGTc                                                                                                                              >  2:2037305/1‑140 (MQ=255)
         tCCACCAACCGAAAGATGAACTGGGGGAGCTGGCGATTGATGTACTCATCCATCGGATAACCCAGCCGACCCTTCAGCAACAACGATTACAACTTACTCCGATTCTGATGGAACGCGGTTCGGCTTAGATTTACGCTGTc                                                                                                                              >  1:1614509/1‑140 (MQ=255)
              caACCGAAAGATGAACTGGGGGAGCTGGCGATTGATGTACTCATCCATCGGATAACCCAGCCGACCCTTCAGCAACAACGATTACAACTTACTCCGATTCTGATGGAACGCGGTTCGGCTTAGATTTACGCTGTCTTTTg                                                                                                                         >  1:5680434/1‑140 (MQ=255)
              caACCGAAAGATGAACTGGGGGAGCTGGCGATTGATGTACTCATCCATCGGATAACCCAGCCGACCCTTCAGCAACAACGATTACAACTTACTCCGATTCTGATGGAACGCGGTTCGGCTTAGATTTACGCTGTCTTTTg                                                                                                                         >  1:4834961/1‑140 (MQ=255)
              caACCGAAAGATGAACTGGGGGAGCTGGCGATTGATGTACTCATCCATCGGATAACCCAGCCGACCCTTCAGCAACAACGATTACAACTTACTCCGATTCTGATGGAACGCGGTTCGGCTTAGATTTACGCTGTCTTTTg                                                                                                                         >  1:4647636/1‑140 (MQ=255)
                aCCGAAAGATGAACTGGGGGAGCTGGCGATTGATGTACTCATCCATCGGATAACCCAGCCGACCCTTCAGCAACAACGATTACAACTTACTCCGATTCTGATGGAACGCGGTTCGGCTTAGATTTACGCTGTCTTTTGAt                                                                                                                       >  2:1494559/1‑140 (MQ=255)
                aCCGAAAGATGAACTGGGGGAGCTGGCGATTGATGTACTCATCCATCGGATAACCCAGCCGACCCTTCAGCAACAACGATTACAACTTACTCCGATTCTGATGGAACGCGGTTCGGCTTAGATTTACGCTGTCTTTTGAt                                                                                                                       <  1:784653/140‑1 (MQ=255)
                aCCGAAAGATGAACTGGGGGAGCTGGCGATTGATGTACTCATCCATCGGATAACCCAGCCGACCCTTCAGCAACAACGATTACAACTTACTCCGATTCTGATGGAACGCGGTTCGGCTTAGATTTACGCTGTCTTTTGAt                                                                                                                       <  1:4235350/140‑1 (MQ=255)
                aCCGAAAGATGAACTGGGGGAGCTGGCGATTGATGTACTCATCCATCGGATAACCCAGCCGACCCTTCAGCAACAACGATTACAACTTACTCCGATTCTGATGGAACGCGGTTCGGCTTAGATTTACGCTGTCTTTTGAt                                                                                                                       >  1:4419568/1‑140 (MQ=255)
                aCCCAAAGATTTACAGGGGGTGCTTGCGATTTATGTACTCATCCATAGGATTAACCATCCGACCCTTCAGCAACAACGATTACAACTTACTCCGATTCTGATGGAACGCGGTTCGGCTTAGATTTACGCTGTCTTTTGAt                                                                                                                       <  2:1345072/140‑1 (MQ=255)
                 ccGAAAGATGAACTGGGGGAGCTGGCGATTGATGTACTCATCCATCGGATAACCCAGCCGACCCTTCAGCAACAACGATTACAACTTACTCCGATTCTGATGGAACGCGGTTCGGCTTAGATTTACGCTGTCTTTTGATc                                                                                                                      <  1:3416565/140‑1 (MQ=255)
                 ccGAAAGATGAACTGGGGGAGCTGGCGATTGATGTACTCATCCATCGGATAACCCAGCCGACCCTTCAGCAACAACGATTACAACTTACACCGATTCTGATGGAACGCGGTTCGGCTTAGATTAACGCTGTCTTTTGATc                                                                                                                      >  1:8333300/1‑140 (MQ=255)
                      aGATGAACTGGGGGAGCTGGCGATTGATGTACTCATCCATCGGATAACCCAGCCGACCCTTAAGCAACAACGATTACAACTTACTCCGAGTCTGATGGATCGCGGTTCGGCTTAGCTTTACGCTGTCTTTTGATAAAgtt                                                                                                                 >  2:6792445/1‑140 (MQ=255)
                           aaCTGGGGGAGCTGGCGATTGATGTACTCATCCATCGGATAACCCAGCCGACCCTTCAGCAACAACGATTACAACTTACTCCGATTCTGATGGAACGCGGTTCGGCTTAGATTTACGCTGTCTTTTGATCAAATTATTAc                                                                                                            >  2:2320564/1‑140 (MQ=255)
                           aaCTGGGGGAGCTGGCGATTGATGTACTCATCCATCGGATAACCCAGCCGACCCTTCAGCAACAACGATTACAACTTACTCCGATTCTGATGGAACGCGGTTCGGCTTAGATTTACGCTGTCTTTTGATCAAATTATTAc                                                                                                            >  2:544745/1‑140 (MQ=255)
                           aaCTGGGGGAGCTGGCGATTGATGTACTCATCCATCGGATAACCCAGCCGACCCTTCAGCAACAACGATTACAACTTACTCCGATTCTGATGGAACGCGGTTCGGCTTAGATTTACGCTGTCTTTTGATCAAATTATTAc                                                                                                            >  1:1247169/1‑140 (MQ=255)
                              tGGGGGAGCTGGCGATTGATGTACTCATCCATCGGATAACCCAGCCGACCCTTCAGCAACAACGATTACAACTTACTCCGATTCTGATGGAACGCGGTTCGGCTTAGATTTACGCTGTCTTTTGATCAAATTATTACCAt                                                                                                         <  2:7854969/140‑1 (MQ=255)
                                 gggAGCTGGCGATTGATGTACTCATCCATCGGATAACCCAGCCGACCCTTCAGCAACAACGATTACAACTTACTCCGATTCTGATGGAACGCGGTTCGGCTTAGATTTACGCTGTCTTTTGATCAAATTATTACCATCgg                                                                                                      <  1:7042982/140‑1 (MQ=255)
                                  ggAGCTGGCGATTGATGTACTCATCCATCGGATAACCCAGCCGACCCTTCAGCAACAACGATTACAACTTACTCCGATTCTGATGGAACGCGGTTCGGCTTAGATTTACGCTGTCTTTTGATCAAATTATTACCATCGgt                                                                                                     >  1:8557348/1‑140 (MQ=255)
                                  ggAGCTGGCGATTGATGTACTCATCCATCGGATAACCCAGCCGACCCTTCAGCAACAACGATTACAACTTACTCCGATTCTGATGGAACGCGGTTCGGCTTAGATGTACGCTGTCTTTTGATCAAATTATTACCATCGgt                                                                                                     >  2:3774942/1‑140 (MQ=255)
                                     gCTGGCGATTGATGTACTCATCCATCGGATAACCCAGCCGACCCTTCAGCAACAACGATTACAACTTACTCCGAGTCTGATGGAACGCGGTTCGGCTTAGATTTACGCTGTCTTTTGATCAAATTATTACCATCGgttgt                                                                                                  >  1:6671691/1‑140 (MQ=255)
                                            aTTGATGTACTCATCCATCGGATAACCCAGCCGACCCTTCAGCAACAACGATTACAACTTACTCCGATTCTGATGGAACGCGGTTCGGCTTAGATTTACGCAGTCTTTTGATCAAATTATTACCATCGGTTGTTTTCAGa                                                                                           <  1:2755406/140‑1 (MQ=255)
                                             ttGATGTACTCATCCATCGGATAACCCAGCCGACCCTTCAGCAACAACGATTACAACTTACTCCGATTCTGATGGAACGCGGTTCGGCTTAGATTTACGCTGTCTTTTGATCAAATTATTACCATCGGTTGTTTTCAGaa                                                                                          <  2:4819130/140‑1 (MQ=255)
                                                       catccatcGGATAACCCAGCCGACCCTTCAGCAACAACGATTACAACTTACTCCGATTCTGATGGAACGCGGTTCGGCTTAGATTTACGCTGTCTTTTGATCAAATTATTACCATCGGTTGTTTTCAGAAGCATGAACAt                                                                                >  1:196325/1‑140 (MQ=255)
                                                        atccatcGGATAACCCAGCCGACCCTTCAGCAACAACGATTACAACTTACTCCGATTCTGATGGAACGCGGTTCGGCTTAGATTTACGCTGTCTTTTGATCAAATTATTACCATCGGTTGTTTTCAGAAGCATGAACAtt                                                                               >  1:2540770/1‑140 (MQ=255)
                                                        atccatcGGATAACCCAGCCGACCCTTCAGCAACAACGATTACAACTTACTCCGATTCTGATGGAACGCGGTTCGGCTTAGATTTACGCTGTCTTTTGATCAAATTATTACCATCGGTTGTTTTCAGAAGCATGAACAtt                                                                               >  1:4375960/1‑140 (MQ=255)
                                                        atccatcGGATAACCCAGCCGACCCTTCAGCAACAACGATTACAACTTACTCCGATTCTGATGGAACGCGGTTCGGCTTAGATTTACGCTGTCTTTTGATCAAATTATTACCATCGGTTGTTTTCAGAAGCATGAACAtt                                                                               >  1:3215484/1‑140 (MQ=255)
                                                         tccatcGGATAACCCAGCCGACCCTTCAGCAACAACGATTACAACTTACTCCGATTCTGATGGAACGCGGTTCGGCTTAGAATTACGCTGTCTTTTGATCAAATTATTACCATCGGTTGTTTTCAGAAGCATGAACATtg                                                                              <  1:5434996/140‑1 (MQ=255)
                                                          ccatcGGATAACCCAGCCGACCCTTCAGCAACAACGATTACAACTTACTCCGATTCTGATGGAACGCGGTTCGGCTTAGATTTACGCTGTCTTTTGATCAAATTATTACCATCGGTTGTTTTCAGAAGCATGAACATtgc                                                                             >  1:6315406/1‑140 (MQ=255)
                                                          ccatcGGATAACCCAGCCGACCCTTCAGCAACAACGATTACAACTTACTCCGATTCTGATGGAACGCGGTTCGGCTTAGATTTACGCTGTCTTTTGATCAAATTATTACCATCGGTTGTTTTCAGAAGCATGAACATtgc                                                                             >  1:4929963/1‑140 (MQ=255)
                                                          ccatcGGATAACCCAGCCGACCCTTCAGCAACAACGATTACAACTTACTCCGATTCTGATGGAACGCGGTTCGGCTTAGATTTACGCTGTCTTTTGATCAAATTATTACCATCGGTTGTTTTCAGAAGCATGAACATtgc                                                                             >  2:1577202/1‑140 (MQ=255)
                                                          ccatcGGATAACCCAGCCGACCCTTCAGCAACAACGATTACAACTTACTCCGATTCTGATGGAACGCGGTTCGGCTTAGATTTACGCTGTCTTTTGATCAAATTATTACCATCGGTTGTTTTCAGAAGCATGAACATtgc                                                                             >  2:2726298/1‑140 (MQ=255)
                                                           catcGGATAACCCAGCCGACTCTTCAGCAACAACGATTACAACTTACTCCGATTCTGATGGAACGCGGTTCGGCTTAGATTTACGCTGTCTTTTGATCAAATTATTACCATCGGTTGTTTTCAGAAGCATGAACATtgct                                                                            >  1:8146881/1‑140 (MQ=255)
                                                           catcGGATAACCCAGCCGACCCTTCAGCAACAACGATTACAACTTACTCCGATTCTGATGGAACGCGGTTCGGCTTAGATTTACGCTGTCTTTTGATCAAATTATTACCATCGGTTGTTTTCAGAAGCATGAACATtgct                                                                            >  1:1674550/1‑140 (MQ=255)
                                                             tcGGATAACCCAGCCGACCCTTCAGCAACAACGATTACAACTTACTCCGATTCTGATGGAACGCGGTTCGGCTTAGATTTACGCTGTCTTTTGATCAAATTATTACCATCGGTTGTTTTCAGAAGCATGAACATtgctgc                                                                          >  1:3542974/1‑140 (MQ=255)
                                                                  tAACCCAGCCGACCCTTCAGCAACAACGATTACAACTTCCTCCGATTCTGATGGAACGCGGTTCGGCTTAGATTTACGCTGTCTTTTGATCAAATTATTACCATCGGTTGTTTTCAGAAGCATGAACATTGCTGCTGAAg                                                                     <  1:6454312/140‑1 (MQ=255)
                                                                   accccAGCCGCCCCTTCAGCAACAACGATTACAACTAACTCCGATTCTGATGGAACGCGGTTCGGCTTAGATTTACGCTGTCTTTTGATCAAATTATTACCCTCGGTTGTTTTCAGAAGCATGAACATTGCTGCTGAAGc                                                                    <  1:6849500/138‑1 (MQ=255)
                                                                   aaCCCAGCCGACCCTTCAGCAACAACGATTACAACTTACTCCGATTCTGATGGAACGCGGTTCGGCTTAGATTTACGCTGTCTTTTGATCAAATTATTACCATCGGTTGTTTTCAGAAGCATGAACATTGCTGCTGAAGc                                                                    <  2:5926955/140‑1 (MQ=255)
                                                                   aaCCCAGCCGACCCTTCAGCAACAACGATTACAACTTACTCCGATTCTGATGGAACGCGGTTCGGCTTAGATTTACGCTGTCTTTTGATCAAATTATTACCATCGGTTGTTTTCAGAAGCATGAACATTGCTGCTGAAGc                                                                    <  1:89643/140‑1 (MQ=255)
                                                                   aaCCCAGCCGACCCTTCAGCAACAACGATTACAACTTACTCCGATTCTGATGGAACGCGGTTCGGCTTAGATTTACGCTGTCTTTTGATCAAATTATTACCATCGGTTGTTTTCAGAAGCATGAACATTGCTGCTGAAGc                                                                    <  1:9156156/140‑1 (MQ=255)
                                                                    aCCCAGCCGACCCTTCAGCAACAACGATTACAACTTACTCCGATTCTGATGGAACGCGGTTCGGCTTAGATTTACGCTGTCTTTTGATCAAATTATTACCATCGGTTGTTTTCAGAAGCATGAACATTGCTGCTGAAGc                                                                    >  2:7973282/1‑139 (MQ=255)
                                                                    aCCCAGCCGACCCTTCAGCAACAACGATTACAACTTACTCCGATTCTGATGGAACGCGGTTCGGCTTAGATTTACGCTGTCTTTTGATCAAATTATTACCATCGGTTGTTTTCAGAAGCATGAACATTGCTGCTGAAGCa                                                                   >  2:4318599/1‑140 (MQ=255)
                                                                     cccAGCCGACCCTTCAGCAACAACGATTACAACTTACTCCGATTCTGATGGAACGCGGTTCGGCTTAGATTTACGCTGTCTTTTGATCAAATTATTACCATCGGTTGTTTTCAGAAGCATGAACATTGCTGCTGAAGCaa                                                                  >  1:1018050/1‑140 (MQ=255)
                                                                      ccAGCCGACCCTTCAGCAACAACGATTACACCTTACTCCGATTCTGATGGAACGCGGTTCGGCTTAGATTTACGCTGTCTTTTGAACAAATTATTACCATCGGTTGTTTTCAGAAGCATGAACATTGCTGCTGAAGCAAc                                                                 <  2:3453847/140‑1 (MQ=255)
                                                                      ccAGCCGACCCTGCATCACCAACCATTACAACCTCCTCCGATTCTGAGGGAACGCGGTACGGCGTAGAATTACGCTGTCTTTTGATCAAATTAGGCCCATCGGTTGTTTTCAGAAGCATGAACATTGCTGCTGAAGCAAc                                                                 <  2:6456830/140‑1 (MQ=255)
                                                                        aGCCGACCCTTCAGCAACAACGATTACAACTTACTCCGATTCTGATGGAACGCGGTTCGGCTTAGATTTACGCTGTCTTTTGATCAAATTATTACCATCGGTTGTTTTCAGAAGCATGAACATTGCTGCTGAAGCAACAg                                                               <  1:3782905/140‑1 (MQ=255)
                                                                        aGCCGACCCTTCAGCAACAACGATTACAACTTACTCCGATTCTGATGGAACGCGGTTCGGCTTAGATTTACGCTGTCTTTTGATCAAATTATTACCATCGGTTGTTTTCAGAAGCATGAACATTGCTGCTGAAGCAACAg                                                               >  1:1148657/1‑140 (MQ=255)
                                                                          ccGACCCTTCAGCAACAACGATTACAACTTACTCCGATTCTGATGGAACGCGGTTCGGCTTAGATTTACGCTGTCTTTTGATCAAATTATTACCATCGGTTGTTTTCAGAAGCATGAACATTGCTGCTGAAGCAACAGta                                                             <  2:7652420/140‑1 (MQ=255)
                                                                           cGACCCTTCAGCAACAACGATTACAACTTACTCCGATTCTGATGGAACGCGGTTCGGCTTAGATTTACGCTTTCTTTTGATCAAATTATTACCATCGGTTGTTTTCAGAAGCATGATCATTGCTGCTGAAGCAACAGtaa                                                            >  1:8838444/1‑140 (MQ=255)
                                                                               ccTTCAGCAACAACGATTACAACTTACTCCGATTCTGATGGAACGCGGTTCGGCTTAGATTTACGCTGTCTTTTGATCAAATTATTACCATCGGTTGTTTTCAGAAGCATGAACATTGCTGCTGAAGCAACAGTaataat                                                        >  2:4613359/1‑140 (MQ=255)
                                                                               ccTTCAGCAACAACGATTACAACTTACTCCGATTCTGATGGAACGCGGTTCGGCTTAGATTTACGCTGTCTTTTGATCAAATTATTACCATCGGTTGTTTTCAGAAGCATGAACATTGCTGCTGAAGCAACAGTaataat                                                        >  2:1746683/1‑140 (MQ=255)
                                                                                  tCAGCAACAACGATTACAACTTACTCCGATTCTGATGGAACGCGGTTCGGCTTAGATTTACGCTGTCTTTTGATCAAATTATTACCATCGGTTGTTTTCAGAAGCATGAACATTGCTGCTGAAGCAACAGTAATAATGcc                                                     <  2:3627083/140‑1 (MQ=255)
                                                                                    aGCAACAACGATGACAACTTACTCCGATTCTGATGGAACGCGGTTCGGCTTAGATTTACGCTGTCTTTTGATCAAATTATTACCATCGGTTGTTTTCAGAAGCATGAACATTGCTGCTGAAGCAACAGTAATAATGCCCa                                                   <  2:6717532/140‑1 (MQ=255)
                                                                                     gCAACACCGATTACAACTTCCTCCGATTCTGCGGGTCCGCGGTTCGGCTTAGATTTACGCTGTCTTGTGATCAAATTATTCCCAACGGTTGTTTTCAGCCGCATGAACATTGCTGCTGAAGCAACAGTAATAAGGCCCAt                                                  <  2:4523965/140‑1 (MQ=255)
                                                                                     gCAACAACGATTACAACTTACTCCGATTCTGATGGAACGCGGTTCGGCTTAGATTTACGCTGTCTTTTGATCAAATTATTACCATCGGTTGTTTTCAGAAGCATGAACATTGCTGCTGAAGCAACAGTAATAATGCCCAt                                                  <  2:9374469/140‑1 (MQ=255)
                                                                                      caacaaCGATTACAACTTACTCCGATTCTGATGGAACGCGGTTCGGCTTAGATTTACGCTGTCTTTTGATCAAATTATTACCATCGGTTGTTTTCAGAAGCATGAACATTGGTGCTGAAGCAACAGTAATAATGCCCAtt                                                 <  2:3295187/140‑1 (MQ=255)
                                                                                      caacaaCGATTACAACTTACTCCGATTCTGATGGAACGCGGTTCGGCTTAGATTTACGCTGTCTTTTGATCAAATTATTACCATCGGTTGTTTTCAGAAGCATGAACATTGCTGCTGAAGCAACAGTAATAATGCCCAtt                                                 <  1:9048055/140‑1 (MQ=255)
                                                                                        acaacGATTACAACTTACTCCGATTCTGATGGAACGCGGTTCGGCTTAGATTTACGCTGTCTTTTGATCAAATTATTACCATCGGTTGTTTTCAGAAGCATGAACATTGCTGCTGAAGCAACAGTAATAATGCCCATtgt                                               <  2:8382185/140‑1 (MQ=255)
                                                                                         caacGATTACAACTTACTCCGATTCTGATGGAACGCGGTTCGGCTTAGATTTTCGCTGTCTTTTGATCAAATTATTACCATCGGTTGTTTTCAGAAGCATGAACATTGCTGCTGAAGCACCAGTAATAATGCCCAGtgtg                                              >  1:9535119/1‑140 (MQ=255)
                                                                                         caacGATTACAACTTACTCCGATTCTGATGGAACGCGGTTCGGCTTAGATTTACGCTGTCTTTTGATCAAATTATTACCATCGGTTGTTTTCAGAAGCATGAACATTGCTGCTGAAGCAACAGTAATAATGCCCATtgag                                              >  2:6236081/1‑138 (MQ=255)
                                                                                          cacGATTACAACGTACTCCGATTCTGATGGAACGCGGTACGGCTTAGATTTACGCTGTCTTTTGATCAAAGTATTACCATCGGTTGTTTTCAGAAGCATGAACATTGCTGCTGAAGCAACAGTAATAATGCCCATTGTGa                                             <  2:1644838/139‑1 (MQ=255)
                                                                                          aacGATTACAACTTACTCCGATTCTGATGGAACGCGGTTCGGCTTAGATTTACGCTGTCTTTTGATCAAATTATTACCATCGGTTGTTTTCAGAAGCATGAACATTGCTGCTGAAGCAACAGTAATAATGCCCATTGTGa                                             <  1:5846673/140‑1 (MQ=255)
                                                                                          aacGATTACAACTTACTCCGATTCTGATGGAACGCGGTTCGGCTTAGATTTACGCTGTCTTTTGATCAAATTATTACCATCGGTTGTTTTCAGAAGCATGAACATTGCTGCTGAAGCAACAGTAATAATGCCCATTGTGa                                             >  2:5097525/1‑140 (MQ=255)
                                                                                          aacGATTACAACTTACTCCGATTCTGATGGAACGCGGGTCGGCTTAGATTTACGCTGTCTTTTGATCAAATTATTACCATCGGTTGTTTTCAGAAGCATGAACATTGCTGCTGAAGCAACAGTAATAATGCCCATTGTGa                                             <  1:5189502/140‑1 (MQ=255)
                                                                                           acGATTACAACTTACTCCGATTCTGATGGAACGCGGTTCGGCTTAGATTTACGCTGTCTTTTGATCAAATTATTACCATCGGTTGTTTTCAGAAGCATGAACATTGCTGCTGAAGCAACAGTAATAATGCCCATTGTGAt                                            >  1:2879582/1‑140 (MQ=255)
                                                                                           acGATTACAACTTACTCCGATTCTGATGGAACGCGGTTCGGCTTAGATTTACGCTGTCTTTTGATCAAATTATTACCATCGGTTGTTTTCAGAAGCATGAACATTGCTGCTGAAGCAACAGTAATAATGCCCATTGTGAt                                            <  1:7473341/140‑1 (MQ=255)
                                                                                            cGATTACAACTTACTCCGATTCTGATGGAACGCGGTTCGGCTTAGATTTACGCTGTCTTTTGATCAAATTATTACCATCGGTTGTTTTCAGAAGCATGAACATTGCTGCTGAAGCAACAGTAATAATGCCCATTGTGATa                                           <  1:2264476/140‑1 (MQ=255)
                                                                                             gATTACACCTTACTACGATTCTGAGGGATCGAGGTTCGGCTTAGATTTACGCTGTCTTTTGATCTATTTTTTACCATCGTTTGTTTTCTGAAGCATGAACATTGCTTCTGAAGAAACAGTAATAATGCCCATTGTGATaa                                          <  2:4013261/140‑1 (MQ=255)
                                                                                             gATTACAACTTACTCCGATTCTGATGGAACGCGGTTCGGCTTAGATTTACGCTGTCTTTTGATCAAATTATTACCATCGGTTGTTTTCAGAAGCATGAACATTGCTGCTGAAGCAACAGTAATAATGCCCATTGTGATaa                                          <  1:5102932/140‑1 (MQ=255)
                                                                                             gATTACAACTTACTCCGATTCTGATGGAACGCGGTTCGGCTTAGATTTACGCTGTCTTTTGATCAAATTATTACCATCGGTTGTTTTCAGAAGCATGAACATTGCTGCTGAAGCAACAGTAATAATGCCCATTGTGATaa                                          <  2:7269118/140‑1 (MQ=255)
                                                                                             gATTACAACTTACTCCGAGTCTGATGGAACGCGGTTCTGCTTAGAGTTACGCCGTCTTTTGATCAAATTATTACCCTCGGTTGTTTTCAGAAGCATGAACATTGCTGCTGAATCAACAGTCATAATGCCCATTGTGATaa                                          <  2:2561569/140‑1 (MQ=255)
                                                                                              aTTACAACTTACTCCGATTCTGATGGAACGCGGTTCGGCTTAGATTTACGCTGTCTTTTGATCAAATTATTACCATCGGTTGTTTTCAGAAGCATGAACATTGCTGCTGAAGCAACAGTAATAATGCCCATTGTGATaaa                                         >  2:2173513/1‑140 (MQ=255)
                                                                                                tactacTTACTCCGATTCTGATGGAACGCGGTTCGGCTTAGATTTTCGCTGTCTTTTGATCAAATTATTACCATCGGTTGTTTTCAGAAGCATGAACATTGCTGCTGAAGCAACAGTTATAATGCCCATTGTGATAAACg                                       <  2:7181957/140‑1 (MQ=255)
                                                                                                tACAACTTACTCCGATTCTGATGGAACGCGGTTCGGCTTAGATTTACGCTGTCTTTTGATCAAATTATTACCATCGGTTGTTTTCAGAAGCATGAACATTGCTGCTGAAGCAACAGTAATAATGCCCATTGTGATAAACg                                       <  2:7535017/140‑1 (MQ=255)
                                                                                                  cAACTTACTCCGATTCTGATGGAACGCGGTTCGGCTTAGATTTACGCTGTCTTTTGATCAAATTATTACCATCGGTTGTTTTCAGAAGCATGAACATTGCTGCTGAAGCAACAGTAATAATGCCCATTGTGATAAACGta                                     <  1:2087176/140‑1 (MQ=255)
                                                                                                        aCTCCGATTCTGATGGAACGCGGTTCGGCTTAGATTTACGCTGTCTTTTGATCAAATTATTACCATCGGTTGTTTTCAGAAGCATGAACATTGCTGCTGAAGCAACAGTAATAATGCCCATTGTGATAAACGTATAGTgg                               >  2:2941435/1‑140 (MQ=255)
                                                                                                        aCTCCGATTCTGATGGAACGCGGTTCGGCTCAGATTTACGCTGTCTTTTGATCAAATTATTACCATCGGTTGTTTTCAGAAGCATGAACATTGCTGCTGAAGCAACAGTAATAATGCCCATTGTGATAAACGTATAGTgg                               >  1:4537072/1‑140 (MQ=255)
                                                                                                         cTCCGATTCTGATGGAACGCGGTTCGGCTTAGATTTACGCTGTCTTTTGATCAAATTATTACCATCGGTTGTTTTCAGAAGCATGAACATTGCTGCTGAAGCAACAGTAATAATGCCCATTGTGATAAACGTATAGTGGa                              >  2:3098909/1‑140 (MQ=255)
                                                                                                              aTTCTGATGGAACGCGGTTCGGCTTAGATTTACGCTGTCTTTTGATCAAATTATTACCATCGGTTGTTTTCAGAAGCATGAACATTGCTGCTGAAGCAACAGTAATAATGCCCATTGTGATAAACGTATAGTGGAATTGt                         >  1:8666471/1‑140 (MQ=255)
                                                                                                               ttCTGATGGAACGCGGTTCGGCTTAGATTTACGCTGTCTTTTGATCAAATTATTACCATCgg                                                                                                      >  2:7018938/1‑62 (MQ=255)
                                                                                                               ttCTGATGGAACGCGGTTCGGCTTAGATTTACGCTGTCTTTTGATCAAATTATTACCATCGGTTGTTTTCAGAAGCATGAACATTGCTGCTGAAGCAACAGTAATAATGCCCATTGTGATAAACGTATAGTGGAATTGtt                        >  2:1200256/1‑140 (MQ=255)
                                                                                                               ttCTGATGGAACGCGGTTCGGCTTAGATTTACGCTGTCTTTTGATCAAATTATTACCATCGGTTGTTTTCAGAAGCATGAACATTGCTGCTGAAGCAACAGTAATAATGCCCATTGTGATAAACGTATAGTGGAATTGtt                        >  2:1958619/1‑140 (MQ=255)
                                                                                                               ttCTGATGGAACGCGGTTCGGCTTAGATTTACGCTGTCTTTTGATCAAATTATTACCATCGGTTGTTTTCAGAAGCATGAACATTGCTGCTGAAGCAACAGTAATAATGCCCATTGTGATAAACGTATAGTGGAATTGtt                        >  2:516919/1‑140 (MQ=255)
                                                                                                                   gATGGAACGCGGTTCGGCTTAGATTTACGCTGTCTTTTGATCAAATTACTACCATCGGTTGTTTTCAGAAGCATGAACATTGCTGCTGAAGCAACAGTAATAATGCCCATTGTGATAAACGTATAGTGGAATTGTTCGAc                    >  1:7286043/1‑140 (MQ=255)
                                                                                                                    aTGGAACGCGGTTCGGCTTAGATTTACGCTGTCTTTTGATCAAATTATTACCATCGGTTGTTTTCAGAAGCATGAACATTGCTGCTGAAGCAACAGTAATAATGCCCATTGTGATAAACGTATAGTGGACTTGTTCGACa                   >  2:8123693/1‑140 (MQ=255)
                                                                                                                     tGGAACGCGGTTCGGCTTAGATTTACGCTGTCTTTTGATCAAATTATTACCATCGGTTGTTTTCAGAAGCATGAACATTGCTGCTGAAGCAACAGTAATAATGCCCATTGTGATAAACGTATAGTGGAATTGTTCGACAg                  <  1:8407393/140‑1 (MQ=255)
                                                                                                                        aaCGCGGTTCGGCTTAGATTTACGCTGTCTTTTGATCAAATTATTACCATCGGTTGTTTTCAGAAGCATGAACATTGCTGCTGAAGCAACAGTAATAATGCCCATTGTGATAAACGTATAGTGGAATTGTTCGACAGTCg               >  1:8064255/1‑140 (MQ=255)
                                                                                                                         aCGCGGTTCGGCTTAGATTTACGCTGTCTTTTGATCAAATTATTACCATCGGTTGTTTTCAGAAGCATGAACATTGCTGCTGAAGCAACAGTAATAATGCCCATTGTGATAAACGGATAGTGGAATTGTTCGACAGTCGt              >  1:5641505/1‑140 (MQ=255)
                                                                                                                            cgGTTCGGCTTAGATTTACGCTGTCTTTTGATCAAATTATTACCATCGGTGGTATTCAGAAGCATGAACATTGCTGCTGAAGCAACAGTAATAATGCCCATTGTGATAAACGTATAGTGGAATTGTTCGACAGTCGTtgt           <  2:336856/140‑1 (MQ=255)
                                                                                                                              gTTCGGCTTAGATTTACGCTGTCTTTTGATCAAATTATTACCATCGGTTGTTTTCAGAAGCATGAACATTGCTGCTGAAGCAACAGTAATAATGCCCATTGTGATAAACGTATAGTGGAATTGTTCGACAGTCGTTGTGc         >  2:8559925/1‑140 (MQ=255)
                                                                                                                               ttCGGCTTAGATTTACGCTGTCTTTTGATCAAATTATTACCATCGGTTGTTTTCAGAAGCATGAACATTGCTGCTGAAGCAACAGTAATAATGCCCATTGTGATAAACGTATAGTGGAATTGTTCGACAGTCGTTGTGcc        <  1:3919159/140‑1 (MQ=255)
                                                                                                                                        |                                                                                                                                        
TAACCACTATCCACCAACCGAAAGATGAACTGGGGGAGCTGGCGATTGATGTACTCATCCATCGGATAACCCAGCCGACCCTTCAGCAACAACGATTACAACTTACTCCGATTCTGATGGAACGCGGTTCGGCTTAAATTTACGCTGTCTTTTGATCAAATTATTACCATCGGTTGTTTTCAGAAGCATGAACATTGCTGCTGAAGCAACAGTAATAATGCCCATTGTGATAAACGTATAGTGGAATTGTTCGACAGTCGTTGTGCCTTCCAT  >  NZ_CP025268/3941002‑3941274

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑