Predicted mutation
evidence seq id position mutation annotation gene description
RA NZ_CP025268 3,958,794 A→G *515* (TAA→TAG)  ilvA → threonine ammonia‑lyase, biosynthetic

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0252683,958,7940AG99.7% 1269.1 / NA 374*515* (TAA→TAG) ilvAthreonine ammonia‑lyase, biosynthetic
Reads supporting (aligned to +/- strand):  ref base A (0/0);  major base G (196/176);  minor base T (1/0);  total (198/176)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 375 total aligned reads displayed.

ACCGACTACGGGCGCGTACTGGCGGCGTTCGAACTTGGCGACCATGAACCGGATTTCGAAACCCGGCTGAATGAGCTGGGCTACGATTGCCACGACGAAACCAATAACCCGGCGTTCAGGTTCTTTTTGGCGGGTTAAGGAAAAATGCCTGATAGCGCTTCGCTTATCAGGCCTACCCGCGCGACAACGTCATTTGTGGTTCGGCAAAATCTTCCAGAATGCCTCAATTAGCGGCTCATGTAGCCGCTTTTTCTGCGCACACACGCCCAGCTC  >  NZ_CP025268/3958657‑3958929
                                                                                                                                         |                                                                                                                                       
 ccGACTACGGGCGCGTACTGGCGGCGTTCGAACTTGGCGACCATGAACCGGATTTCGAAACCCGGCTGAATGAGCTGGGCTACGATTGCCACGACGAAACCAATAACCCGGCGTTCAGGTTCTTTTTGGCGGGTTAGGGa                                                                                                                                      >  2:8556440/1‑140 (MQ=255)
  cGACTACGGGCGCGTACTGGCGGCGTTCGAACTTGGCGACCATGAACCGGATTTCGAAACCCGGCTGAATGAGCTGGGCTACGATTGCCACGACGAAACCAATAACCCGGCGTTCAGGTTCTTTTTGGCGGGTTAGGGaa                                                                                                                                     <  1:1091531/140‑1 (MQ=255)
   gACTACGGGCGCGTACTGGCGGCGTTCGAACTTGGCGGCCATGAACCGGATTTCGAAACCCGGCTGAATGAGCTGGGCTACGATTGCCACGACGAAACCAATAACCCGGCGTTCAGGGGCTGTGTGGCGGGTGAGGGacc                                                                                                                                    >  1:6828212/1‑138 (MQ=255)
    aCTACGGGCGCGTACTTGCGGCGTTAGAACTTGGCGACCATGAACCGGATTTCGAAACCCGGCTGAATGAGCTGGGCTACGATTGCCACGTCGAAACCAATAACCCGGCGTTAAGGTTCTTTTTGGCGTGTTAGGGaaaa                                                                                                                                   >  2:2508420/1‑140 (MQ=255)
    aCTACGGGCGCGTACTGGCGGCGTTCGAACTTGGCGACCATGAACCGGATTTCGAAACCCGGCTGAATGAGCTGGGCTACGATTGCCACGACGAAACCAATAACCCGGCGTTCAGGTTCTTTTTGGCGGGTTAGGGaaaa                                                                                                                                   >  2:6343115/1‑140 (MQ=255)
    aCTACGGGCGCGTACTGGCGGCGTTCGAACTTGGCGACCATGAACCGGATTTCGAAACCCGGCTGAATGAGCTGGGCTACGATTGCCACGACGAAACCAATAACCCGGCGTTCAGGTTCTTTTTGGCGGGTTAGGGaaaa                                                                                                                                   <  1:3861391/140‑1 (MQ=255)
    aCTACGGGCGCGTACTGGCGGCGTTCGAACTTGGCGACCATGAACCGGATTTCGAAACCCGGCTGAATGAGCTGGGCTACGATTGCCACGACGAAACCAATAACCCGGCGTTCAGGTTCTTTTTGGCGGGTGAGGGaaaa                                                                                                                                   >  1:8677322/1‑140 (MQ=255)
      tACGGGCGCGTACTGGCGGCGTTCGAACTTGGCGACCATGAACCGGATTTCGAAACCCGGCTGAATGAGCTGGGCTACGATTGCCACGACGAAACCAATAACCCGGCGTTCAGGTTCTTTTTGGCGGGTTAGGGAAAAAt                                                                                                                                 >  1:9074037/1‑140 (MQ=255)
        cGGGGGCGTACTGGCGGCGTGCGACCGTGGCGACCATGACCCGGATGTCGAAACCCGGCTGAATGAGCTGGGCTACGCTTGCCCCGTCGAACCCAAGACCCCGGCGTGCAGGTGCGTTTTGGCGGGTGAGGGACAAAGGc                                                                                                                               >  2:6615473/1‑140 (MQ=255)
        cGGGCGCGTACTGGCGGCGTTCGAACTTGGCGACCATGAACCGGATTTCGAAACCCGGCTGAATGAGCTGGGCTCCGATTGCCACGACGAAACCAATAACCCGGCGTTCAGGTTCTTTTTGGCGGGTTAGGGAAAAATGc                                                                                                                               >  2:4744016/1‑140 (MQ=255)
        cGGGCGCGTACTGGCGGCGTTCGAACTTGGCGACCATGAACCGGATTTCGAAACCCGGCTGAATGAGCTGGGCTACGATTGCCACGACGAAACCAATAACCCGGCGTTCAGGTTCTTTTTGGCGGGTTAGGGAAAAATGc                                                                                                                               >  2:2098751/1‑140 (MQ=255)
         gggCGCGTACTGGCGGCGTTCGAACTTGGCGACCATGAACCGGATTTCGAAACCCGGCTGAATGAGCTGGGCTACGATTGCCACGACGAAACCAATAACCCGGCGTTCAGGTTCTTTTTGGCGGGTTAGGGAAAAATGcc                                                                                                                              <  1:3524527/140‑1 (MQ=255)
            cgcgTACTGGCGGCGTTCGAACTTGGCGACCATTCACCGGATTGCGAAACCCGGCTGAATGAGCTGGGCTACGATTGCCACGACGAAACCAATAACCCGGCGTTCAGGTTCTTTTTGGCGGGTTAGGGAAAAATGCCTGa                                                                                                                           <  2:8076550/140‑1 (MQ=255)
             gcgTACTGGCGGCGTTCGAACTTGGCGACCATGAACCGGATTTCGAAACCCGGCTGAATGAGCTGGGCTACGATTGCCACGACGAAACCAATAACCCGGCGTTCAGGTTCTTTTTGGCGGGTTAGGGAAAAATGCCTGAt                                                                                                                          >  1:8902271/1‑140 (MQ=255)
              cgTACTGGCGGCGTTCGAACTTGGCGACCATGTACCGGAGTTCGAAACCCGGCTGAATGAGCTGGTCTACGATTGCCACGACGAAACCAATAACCCGGCGTTCAGGGTCTTTGTGGCGGGTTAGGGGAATATGCCTGagt                                                                                                                         >  2:5450600/1‑138 (MQ=255)
              cgTACTGGCGGCGTTCGAACTTGGCGACCATGAACCGGATTTCGAAACCCGGCTGAATGAGCTGGGCTACGTTGGCCACGACGAAACCAATACCCCGGCGTTCAGGTTCTGTTTGGCGGGGTAGGGCCAAATGCCTgtga                                                                                                                         >  1:3225673/1‑137 (MQ=255)
              cgTACTGGCGGCGTTCGAACTTGGCGACCATGAACCGGATTTCGAAACCCGGCTGAATGAGCTGGGCTACGATTGCCACGACGAAACCAATAACCCGGCGTTCAGGTTCTTTTTGGCGGGTTAGGGAAAAATGCCTGATa                                                                                                                         >  1:7853240/1‑140 (MQ=255)
              cgTACTGGCGGCGTTCGAACTTGGCGACCATGAACCGGATTTCGAAACCCGGCTGAATGAGCTGGGCTACGATTGCCACGACGAAACCAATAACCCGGCGTTCAGGTTCTTTTTGGCGGGTTAGGGAAAAATGCCTGATa                                                                                                                         >  1:8120480/1‑140 (MQ=255)
              cgTACTGGCGGCGTTCGAACTTGGCGACCATGAACCGGATTTCGAAACCCGGCTGAATGAGCTGGGCTACGATTGCCACGACGAAACCAATAACCCGGCGTTCAGGTTCTTTTTGGCGGGTTAGGGAAAAATGCCTGATa                                                                                                                         >  2:5263012/1‑140 (MQ=255)
              cgTACTGGCGGCGTTCGAACTTGGCGACCATGAACCGGATTTCGAAACCCGGCTGAATGAGCTGGGCTACGATTGCCACGACGAAACCAATAACCCGGCGTTCAGGTTCTTTTTGGCGGGTTAGGGAAAAATGCCTGATa                                                                                                                         >  1:394498/1‑140 (MQ=255)
              cgTACTGGCGGCGTTCGAACTTGGCGACCATGAACCGGATTTCGAAACCCGGCTGAATGAGCTGGGCTACGATTGCCACGACGAAACCAATAACCCGGCGTTCAGGTTCTTTTTGGCGGGTTAGGGAAAAATGCCTGATa                                                                                                                         >  1:476187/1‑140 (MQ=255)
              cgTACTGGCGGCGTTCGAACTTGGCGACCATGAACCGGATTTCGAAACCCGGCTGAATGAGCTGGGCTACGATTGCCACGACGAAACCAATAACCCGGCGTTCAGGTTCTGTTTGGCGGGTTAGGGAAAAATGCCTGATa                                                                                                                         >  2:8634421/1‑140 (MQ=255)
              cgTACTGGCGGCGTTCGAACTTGGCGACCATGAACCGGATTTCGAAACCCGGCTGAATGAGCTGGGCTACGATTGCCACGACGAAACCAATAACCCGGCGTTCAGGGTCTTTTTGGCGGGTTAGGGAAAAATGCCTGATa                                                                                                                         >  1:4930069/1‑140 (MQ=255)
              cgTACGGGCGGCGTTCGAACTTGGCGACCATGAACCGGATTTCGAAACCCGGCTGAATGTGCTGGGCTACGTTTGCGACGACGAAACAAATAACCAGGCGTTCAGGTTCTGTTTGGCGGGTTAGGGAAAAATGCCTGAtc                                                                                                                         >  2:3480124/1‑139 (MQ=255)
              cgTAATGGCGGCGTTCGAACTTGGCGACCATGAACCGGATTTCGAAACCCGGCTGAATGAGCTGGGCTACTATTGCCACGACGAAACCAATAACCCGGAGTTCACGTTCTTTTTGGCGGGTTACGGAAAAATGACTGATa                                                                                                                         >  1:1681221/1‑140 (MQ=255)
               gTACTGGCGGCGTTCGAACTTGGCGACCATGAACCGGATTTCGAAACCCGGCTGAATGAGCTGGGCTACGATTGCCACGACGAAACCAATAACCCGGCGTTCAGGTTCTTTTTGGCGGGTTAGGGAAAAATGCCTGATAg                                                                                                                        >  1:9478989/1‑140 (MQ=255)
                tACTGGCGGCGTTCGAACTTGGCGACCATGAACCGGATTTCGAAACCCGGCTGAATGAGCTGGGCTACGATTGCCACGACGAAACCAATAACCCGGCGTTCAGGTTCTTTTTGGCGGGTTAGGGAAAAATGCCTGATAgc                                                                                                                       >  1:3991068/1‑140 (MQ=255)
                tACTGGCGGCGTTCGAACTTGGCGACCATGAACCGGATTTCGAAACCCGGCTGAATGAGCTGGGCTACGATTGCCACGACGAAACCAATAACCCGGCGTTCAGGTTCTTTTTGGCGGGTTAGGGAAAAATGCCTGATAgc                                                                                                                       >  2:3335382/1‑140 (MQ=255)
                 aCTGGCGGCGTTCGAACTTGGCGACCATGAACCGGATGTCGAAACCCGGCTGAATGAGCTGGGCTACGATTGCCACGACGAAACCAATAACCCGGCGTTCAGGTTCTTTTTGGCGGGTTAGGGAAAAATGCCTGATAgcg                                                                                                                      <  2:5995982/140‑1 (MQ=255)
                    ggcggcGTTCGAACTTGGCGACCATGAACCGGATTTCGAAACCCGGCTGAATGAGCTGGGCTACGATTGCCACGACGAAACCAATAACCCGGCGTTCAGGTTCTTTTTGGCGGGTTAGGGAAAAATGCCTGATAGcgctt                                                                                                                   <  2:27198/140‑1 (MQ=255)
                      cggcgTTCGAACTGGGCGACCCTGAACCGGCTTTCGAAACCCGGCTGAATGAGCTGGGCTACGATTGCCACGACGAAACCAATAACCCGGCGTTCAGGTTCTTTTTGGCGGGTTAGGGAAAAATGCCTGATAGcgcttcg                                                                                                                 <  2:6445623/140‑1 (MQ=255)
                       ggcgTTCGAACTTGGCGACCATGAACCGGATTTCGAAACCCGGCTGAATGAGCTGGGCTACGATTGCCACGACGAAACCAATAACCCGGCGTTCAGGTTCTTTTTGGCGGGTTAGGGAAAAATGCCTGATAGcgcttcgc                                                                                                                >  2:495887/1‑140 (MQ=255)
                       ggcgTTCGAACTTGGCGACCATGAACCGGATTTCGAAACCCGGCAGAATGAGCTGGGCTACGATTGCCACGACGAACACAATAACCCGGCGTTCAGGTTCTTTTTGGAGGGTTAGGGAAAAATGCCTGATAGcgcttcgc                                                                                                                >  2:6219458/1‑140 (MQ=255)
                        gcgTTCGAACTTGGCGACCATGAACCGGATTTCGAAACCCGGCTGAATGAGCTGGGCTACGATTGCCACGACGAAACCAATAACCCGGCGTTCAGGTTCTTTTTGGCGGGTTAGGGAAAAATGCCTGATAGcgcttcgct                                                                                                               >  2:8741125/1‑140 (MQ=255)
                         cgTTCGAACTTGGCGACCATGTACCGGATGTCGACTCCCGGCTGAATGAGCTGGGCTACGCTGGCCCCGACGCTAGCAATATCCCGGCGTTCTGGGTCTGTTTGGCGGGTTAGGGAAGAGTGCCTGAGCGcgcttcgctt                                                                                                              >  2:7478891/1‑140 (MQ=255)
                         cgTTCGAACTTGGCGACCATGAACCGGATTTCGAAACCCGGCTGAATGAGCTGGGCTACGATTGCCACGACGAAACCAATAACCCGGCGTTCAGGTTCTTTTTGGCGGGTTAGGGAAAAATGCCTGATAGcgcttcgctt                                                                                                              >  2:2378159/1‑140 (MQ=255)
                          gTTCGATCTTGGCGACCATGAACCGGAGTTCGACTCCCGGCTGAATGAGCTGGGCTTCGATTGCCACGACGAAACCAATCACCCGGCGTTCAGGTTCTTTTTGGCGGGTTAGGGAAAAATGCCTGATAGCGCTTCGCTTa                                                                                                             <  2:8215890/140‑1 (MQ=255)
                          gTTCGAACTTGGCGACCATGAACCGGATTTCGAAACCCGGCTGAATGAGCTGGGCTACGATTGCCACGACGAAACCAATAACCCGGCGTTCAGGTTCTTTTTGGCGGGTTAGGGAAAAATGCCTGATAGCGCGTCGCTTa                                                                                                             >  1:4695714/1‑140 (MQ=255)
                           ttCGAACTTGGCGACCATGAACCGGCTTTCGAAACCCGGCTGAAAGAGCTGGGCTACGATTGCCACGACGAAACCAATAACCCGGCGTTCAGGTTCTTGTTGGCGGGGTAGGGAAAAATGCCTGATAGCGCTTCGCTTAt                                                                                                            >  2:2868424/1‑140 (MQ=255)
                           ttCGAACTTGGCGACCATGAACCGGATTTCGAAACCCGGCTGAATGAGCTGGGCTACGATTGCCACGACGAAACCAATAACCCGGCGTTCAGGTTCTTTTTGGCGGGTTAGGGAAAAATGCCTGATAGCGCTTCGCTTAt                                                                                                            >  2:799271/1‑140 (MQ=255)
                           ttCGAACTTGGCGACCATGAACCGGATTTCGAAACCCGGCTGAATGAGCTGGGCTACGATTGCCACGACGAAACCAATAACCCGGCGTTCAGGTTCTTTTTGGCGGGTTAGGGAAAAATGCCTGATAGCGCTTCGCTTAt                                                                                                            <  2:169845/140‑1 (MQ=255)
                           ttCGAACTTGGCGACCATGAACCGGATTTCGAAACCCGGCTGAATGAGCTGGGCTACGATTGCCACGACGAAACCAATAACCCGGCGTTCAGGTTCTTTTTGGCGGGTTAGGGAAAAATGCCAGATAGcgcttcgctaaa                                                                                                            >  1:5821977/1‑137 (MQ=255)
                           ttCGAACTTGGCGACCATGAACCGGATTTCGAAACCCGGCTGAATGAGATGGGCTACGATTGCCACGACGAAACCAATAACCCGGCGTTCAGTTTATTTTTGGCGGGTTAGGGAAAAATGCCTGATAGCGCTTCGCTTAt                                                                                                            >  1:247660/1‑140 (MQ=255)
                           gtCGAACTTGGCGACCCTGAACCGGATTTCGAAACCCGGCTGAATGAGCTGGGCTACGATTGCCACGACGAAACCAATAACCCGGCGTTCAGGTTCTTTTTGGCGGGTTAGGGAAAAATGCCTGATAGCGCTTCGCTTAt                                                                                                            <  2:4187517/139‑1 (MQ=255)
                                  ttGGCGACCATGAACCGGATTTCGAAACCCGGCTGAATGAGCTGGGCTACGATTGCCACGACGAAACCAATAACCCGGCGTTCAGGTTCTTTTTGGCGGGTTAGGGAAAAATGCCTGATAGCGCTTCGCTTATCAGGCCt                                                                                                     <  1:4263982/140‑1 (MQ=255)
                                   tGGCGACCATGAACCGGATTTCGAAACCCGGCTGAATGAGCTGGGCTACGATTGCCACGACGAAACCAATAACCCGGCGTTCAGGTTCTTTTTGGCGGGTTAGGGAAAATTGCCTGATAGCGCTTCGCTTATCAGGCCTa                                                                                                    >  1:6923491/1‑140 (MQ=255)
                                   tGGCGACCATGAACCGGATTTCGAAACCCGGCTGAATGAGCTGGGCTACGATTGCCACGACGAAACCAATAACCCGGCGTTCAGGTTCTTTTTGGCGGGTTAGGGAAAAATGCCTGATAGCGCTTCGCTTATCAGGCCTa                                                                                                    >  2:4073074/1‑140 (MQ=255)
                                             gAACCGGATTTCGAAACCCGGCTGAATGAGCTGGGCTACGATTGCCACGACGAAACCAATAACCCGGCGTTCAGGTTCTTTTTGGCGGGTTAGGGAAAAATGCCTGATAGCGCTTCGCTTATCAGGCCTACCCGCGCGAc                                                                                          <  1:1662563/140‑1 (MQ=255)
                                             gAACCGGATTTCGAAACCCGGCTGAATGAGCTGGGCTACGATTGCCACGACGAAACCAATAACCCGGCGTTCAGGTTCTTTTTGGCGGGTTAGGGAAAAATGCCTGATAGCGCTTCGCTTATCAGGCCTACCCGCGCGAc                                                                                          <  1:6429475/140‑1 (MQ=255)
                                                ccGGATTTCGAAACCCGGCTGAATGAGCTGGGCTACGATTGCCACGACGAAACCAATAACCCGGCGTTCAGGTTCTTTTTGGCGGGTTAGGGAAAAATGCCTGATAGCGCTTCGCTTATCAGGCCTACCCGCGCGACAAc                                                                                       <  1:5382014/140‑1 (MQ=255)
                                                 cGGATTTCGAAACCCGGCTGAATGAGCTGGGCTACGATTGCCACGACGAAACCAATAACCCGGCGTTCAGGTTCTTTTTGGCGGGTTAGGGAAAAATGCCTGATAGCGCTTCGCTTATCAGGCCTACCCGCGCGACAACg                                                                                      <  1:7125113/140‑1 (MQ=255)
                                                 cGGATTTCGAAACCCGGCTGAATGAGCTGGGCTACGATTGCCACGACGAAACCAATAACCCGGCGTTCAGGTTCTTTTTGGCGGGTTAGGGAAAAATGCCTGATAGCGCTTCGCTTATCAGGCCTACCCGCGCGACAACg                                                                                      <  2:698064/140‑1 (MQ=255)
                                                        cGAAACCCGGCTGAATGAGCTGGGGTACGATTGCCACGACGAAACCAATACCCCGGCGGTCAGGTTCTTTTTGGCGGGTTAGGGCACATTGCCTGAGAGCGCTTCGCTGAGCAGGCCTCCCCGCGCGACACCGGCAGTtg                                                                               >  2:6789072/1‑140 (MQ=255)
                                                         gAAACCCGGCTGAATGAGCTGGGCTACGATTGCCACGACGAAACCAATAACCCGGCGTTCAGGTTCTTTTTGGCGGGTTAGGGAAAAATGCCTGATAGCGCTTCGCTTATCAGGCCTACCCGCGCGACAACGTCATTtgt                                                                              >  2:4557503/1‑140 (MQ=255)
                                                            aCCCGGCTGAATGAGCTGGGCTACGATTGCCACGACGAAACCAATAACCCGGCGTTCAGGTTCTTTTTGGCGGGTTAGGGAAAAATGCCTGATAGCGCTTCGCTTATCAGGCCTACCCGCGCGACAACGTCATTTGTGGt                                                                           <  1:362126/140‑1 (MQ=255)
                                                             cccGGCTGAATGAGCTGGGCTACGATTGCCACGACGAAACCAATAACCCGGCGTTCAGGTTCTATTTGGCGGGTTAGGGAAAAATGCCTGATAGCGCTTCGCTTATCAGGCCTACCCGCGCGACAACGTCATTTGTGGtt                                                                          <  1:2606237/140‑1 (MQ=255)
                                                                    gAATGAGCTGGGCTACGATTGCCACGACGAAACCAATAACCCGGCGTTCAGGTTCTTTTTGGCGGGTTAGGGAAAAATGCCNGATAGCGCTTCGCTTATCAGGCCTACCCGCGCGACAACGTCATTTGTGGTTCGGCaaa                                                                   <  1:5586686/140‑1 (MQ=255)
                                                                     aaTGAGCTGGGCTACGATTGCCACGACGAAACCAATAACCCGGCGTTCAGGTTCTTTTTGGCGGGTTAGGGAAAAATGCCTGATAGCGCTTCGCTTATCAGGCCTACCCGCGCGACAACGTCATTTGTGGTTCGGCaaaa                                                                  <  2:7709416/140‑1 (MQ=255)
                                                                              ggCTACGATTGCCACGACGAAACCAATAACCCGGCGTTCAGGTTCTTTTTGGCGGGTTAGGGAAAAATGCCTGATAGCGCTTCGCTTATCAGGCCTACCCGCGCGACAACGTCATTTGTGGTTCGGCAAAATCTTCCAGa                                                         <  1:1037640/140‑1 (MQ=255)
                                                                              ggCTACGATTGCCACGACGAAACCAATAACCCGGCGTTCAGGTTCTTTTTGGCGGGTTAGGGAAAAATGCCTGATAGCGCTTCGCTTATCAGGCCTACCCGCGCGACAACGTCATTTGTGGTTCGGCAAAATCTTCCAGa                                                         <  1:6629235/140‑1 (MQ=255)
                                                                              ggCTACGATTGCCACGACGAAACCAATAACCCGGCGTTCAGGTTCTTTTTGGCGGGTTAGGGAAAAATGCCTGATAGCGCTTCGCTTATCAGGCCTACCCGCGCGACAACGTCATTTGTGGTTCGGCAAAATCTTCCAGa                                                         <  2:5733801/140‑1 (MQ=255)
                                                                               gCTACGATTGCCACGACGAAACCAATAACCCGGCGTTCAGGGTCTTTTTGGCGGGGTAGGGAAAAATGCCTGATAGCGCTTCGCTTATCAGGCCTACCCGCGCGACAACGTCATTTGTGGTTCGGCAAAATCTTCCCGaa                                                        >  1:179085/1‑140 (MQ=255)
                                                                                 tACGATTGCCACGACGAAACCAATAACCCGGCGTTCAGGTTCTTTTTGGCGGGTTAGGGAAAAATGCCTGATAGCGCTTCGCTTATCAGGCCTACCCGCGCGACAACGTCATTTGTGGTTCGGCAAAATCTTCCAGAATg                                                      <  2:919821/140‑1 (MQ=255)
                                                                                     aTTGCCACGACGAAACCAATAACCCGGCGTTCAGGTTCTTTTTGGCGGGTTAGGGAAAAATGCCTGATAGCGCTTCGCTTATCAGGCCTACCCGCGCGACAACGTCATTTGTGGTTCGGCAAAATCTTCCAGAATGCCTc                                                  >  2:3210372/1‑140 (MQ=255)
                                                                                        gCCACGACGAAACCAATAACCCGGCGTTCAGGTTCTTTTTGGCGGGTTAGGGAAAAATTCCTTATAGCGCTTCGCATATCACGCCTACCCGCGCGACAACGTCAGTTGTGGTTCGGCAAAATCTTCCAGAATGCCTCAAt                                               >  1:5710075/1‑140 (MQ=255)
                                                                                        gCCACGACGAAACCAATAACCCGGCGTTCAGGTTCTTTTTGGCGGGTTAGGGAAAAATGCCTGATAGCGCTTCGCTTATCAGGCCTACCCGCGCGACAACGTCCTTTGTGGTTCGGCAAAATCTTCCAGAATGCCTCAAt                                               >  1:5472911/1‑140 (MQ=255)
                                                                                        gCCACGACGAAACCAATAACCCGGCGTTCAGGTTCTTTTTGGCGGGTTAGGGAAAAATGCCTGATAGCGCTTCGCTTATCAGGCCTACCCGCGCGACAACGTCATTTGTGGTTCGGCAAAATCTTCCAGAATGCCTCAAt                                               >  1:6180733/1‑140 (MQ=255)
                                                                                        gCCACGACGAAACCAATAACCCGGCGTTCAGGTTCTTTTTGGCGGGTTAGGGAAAAATGCCTGATAGCGCTTCGCTGATCAGGCCTACCCGCGCGACAACGTCAGTTGTGGTTCGGCAAAATCTTCCAGAATGCCTCAAt                                               >  1:7631173/1‑140 (MQ=255)
                                                                                        gCCACGACGAAACCAATAACCCGGCGTTCAGGTTCTTTTTGGCGGGTTAGGGAAAAATGCCTGATAGCGCTTCGCGTATCAGGCCTACCCGCGCGACAACGTCATTTGTGGTTCGGCAAAATCTTCCAGAATGCCGCAAt                                               >  1:3159707/1‑140 (MQ=255)
                                                                                         ccACGACGAAACCAATAACCCGGCGTTCAGGTTCTTTTTGGCGGGTTAGGGAAAAATGCCTGATAGCGCTTCGCTTATCAGGCCTACCCGCGCGACAACGTCATTTGTGGTTCGGCAAAATCTTCCAGAATGCCTCAAtt                                              >  2:7169253/1‑140 (MQ=255)
                                                                                         ccACGACGAAACCAATAACCCGGCGTTCAGGTTCTTTTTGGCGGGTTAGGGAAAAATGCCTGATAGCGCTTCGCTTATCAGGCCTACCCGCGCGACAACGTCATTTGTGGTTCGGCAAAATCTTCCAGAATGCCTCAAtt                                              >  1:4556840/1‑140 (MQ=255)
                                                                                          cACGACGAAACCAATAACCCGGCGTTCAGGTTCTTTTTGGCGGGTTAGGGAAAAATGCCTGATAGCGCTTCGCTTATCAGGCCTACCCGCGCGACAACGTCATTTGTGGTTCGGCAAAATCTTCCAGAATGCCTCAATTa                                             <  1:5263012/140‑1 (MQ=255)
                                                                                             gacgaAACCAATAACCCGGCGTTCAGGTTCTTTTTGGCGGGTTAGGGAAAAATGCCTGATAGCGCTTCGCTTATCAGGCCTACCCGCGCGACAACGTCATTTGTGGTTCGGCAAAATCTTCCAGAATGCCTCAATTAGCg                                          >  2:3895724/1‑140 (MQ=255)
                                                                                             gacgaAACCAATAACCCGGCGTTCAGGTTCTTTTTGGCGGGTTAGGGAAAAATGCCTGATAGCGCTTCGCGTATCAGGCCTACCCGCGCGTCAACGTCATTTGTGGGTCGGGAAAATCTTCCAGAATGCGTCGATTAGCg                                          >  1:7375402/1‑140 (MQ=255)
                                                                                              acgaAACCAATAACCCGGCGTTCAGGTTCTTTTTGGCGGGTTAGGGAAAAATGCCTGATAGCGCTTCGCTTATCAGGCCTACCCGCGCGACAACGTCATTTGTGGTTCGGCAAAATCTTCCAGAATGCCTCAATTAGCgg                                         <  2:7670093/140‑1 (MQ=255)
                                                                                                gaAACCAATAACCCGGCGTTCAGGTTCTTTTTGGCGGGTTAGGGAAAAATTCCTGATAGCGCTTCGCTTATCAGGCCTACCCGCGCGACCACGTCATTTGTGGTTCGGCAAAATCTTCCAGAATGCCTCAATTAGCGGCt                                       >  1:2157636/1‑140 (MQ=255)
                                                                                                      aaTAACCCGGCGTTCAGGTTCTTTTTGGCGGGTTAGGGAAAAATGCCTGATAGCGCTTCGCTTATCAGGCCTACCCGCGCGACAACGTCATTTGTGGTTCGGCAAAATCTTCCAGAATGCCTCAATTAGCGGCTCATGTa                                 <  2:1004271/140‑1 (MQ=255)
                                                                                                      aaTAACCCGGCGTTCAGGTTCTTTTTGGCGGGTTAGGGAAAAATGCCTGATAGCGCTTCGCTTATCAGGCCTACCCGCGCGACAACGTCATTTGTGGTTCGGCAAAATCTTCCAGAATGCCTCAATTAGCGGCTCATGTa                                 <  2:7038647/140‑1 (MQ=255)
                                                                                                      aaTAACCCGGCGTTCAGGTTCTTTTTGGCGGGTTAGGGAAAAATGCCTGATAGCGCTTCGCTTATCAGGCCTACCCGCGCGACAACGTCATTTGTGGTTCGGCAAAATCTTCCAGAAGGCCTCAATTAGCGGCTCATGTa                                 <  2:1914956/140‑1 (MQ=255)
                                                                                                        tAACCCGGCGTTCAGGTTCTTTTTGGCGGGTTAGGGAAAAATGCCTGATAGCGCTTCGCTTATCAGGCCTACCCGCGCGACAACGTCATTTGTGGTTCGGCAAAATCTTCCAGAATGCCTCAATTAGCGGCTCATGTAGc                               <  1:2332616/140‑1 (MQ=255)
                                                                                                        tAACCCGGCGTTCAGGTTCTTTTTGGCGGGTTAGGGAAAAATGCCTGATAGCGCTTCGCTTATCAGGCCTACCCGCGCGACAACGTCATTTGTGGTTCGGCAAAATCTTCCAGAATGCCTCAATTAGCGGCTCATGTAGc                               <  1:5117536/140‑1 (MQ=255)
                                                                                                           cccGGCGTTCAGGTTCTTTTTGGCGGGTTAGGGAAAAATGCCTGATAGCGCTTCGCGTATCAGGCCTACCCGCGCGACAACGTCATTTGTGGTTCGGCAAAATCTTCCAGAATGCCTCAATTAGCGGCTCATGGAGCCGc                            >  1:800967/1‑140 (MQ=255)
                                                                                                             cGGCGTTCAGGTTCTTTGTGGCGGGTTAGGGACAAATGCCTGATAGCGCTTCGCGTATCAGGCCTACCCGCGCGACCACGTCATTTGTGGTGCGGCCAAATCTTCCAGAATGCCTCAAGTAGCGGCTCATGTAGCCGCtt                          >  2:1437448/1‑140 (MQ=255)
                                                                                                              ggCGTTCAGGTTCTTTTTGGCGGGTTAGGGAAAAATGCCTGATAGCGCTTCGCTTATCAGGCCTACCCGCGCGACAACGTCATTTGTGGTTCGGCAAAATCTTCCAGAATGCCTCAATTAGCGGCTCATGTAGCCGCtgt                         >  1:9283726/1‑138 (MQ=255)
                                                                                                               gCGTTCAGGTTCTTTTTGGCGGGTTAGGGAAAATTGCCTGATAGCGCTTCGGTTATCAGGCCTACCCCCGCGACACCGGCATTTGTGGTTCGGCAAAATCTTCCCGAATGCCTCAATTAGCGGCTCATGTAGCCGCtttt                        >  1:7002171/1‑140 (MQ=255)
                                                                                                                cGTTCAGGTTCTTTTTGGCGGGTTAGGGAAAAATGCCTGATAGCGCTTCGCTTATCAGGCCTACCCGCGCGACAACGTCATTTGTGGTTCGGCAAAATCTTCCAGAATGCCTCAATTAGCGGCTCATGTAGCCGCttttt                       >  2:4364553/1‑140 (MQ=255)
                                                                                                                   tCAGGTTCTTTTTGGCGGGTTAGGGAAAAATGCCTGATAGCGCTTCGCTTATCAGGCCTACCCGCGCGACAACGTCATTTGTGGTTCGGCAAAATCTTCCAGAATGCCTCAATTAGCGGCTCATGTAGCCGCTTTTTCTg                    <  2:8902271/140‑1 (MQ=255)
                                                                                                                     agggacTTTTTGGCGGGTTAGGGAAACATGCCTGCTAGCGCTGCGCTTAGCAGGCCTACCCGCGCGACAACGTCATTTGTGGTTCGGCACAATCTTCCAGACTGCCTCAATTAGCGGCTCATGTAGCCGCTTTTTCTgcg                  <  2:3847613/135‑1 (MQ=255)
                                                                                                                     aGGTTCTTTTTGGCGGGTTAGGGAAAAATGCCTGATAGCGCTTCGCTTATCAGGCCTACCCGCGCGACAACGTCATTTGTGGTTCGGCAAAATCTTCCAGAATGCCTCAATTAGCGGCTCATGTAGCCGCTTTTTCTgcg                  <  1:1835272/140‑1 (MQ=255)
                                                                                                                     aGGTTCTTTTTGGCGGGTTAGGGAAAAATGCCTGATAGCGCTTCGCTTATCAGGCCTACCCGCGCGACAACGTCATTTGTGGTTCGGCAAAATCTTCCAGAATGCCTCAATTAGCGGCTCATGTAGCCGCTTTTTCTgcg                  <  1:1715275/140‑1 (MQ=255)
                                                                                                                     aGGTTCTTTTTGGCGGGTTAGGGAAAAATGCCTGATAGCGCTTCGCTTATCAGGCCTACCCGCGCGACAACGTCATTTGTGGTTCGGCAAAATCTTCCAGAATGCCTCAAATAGCGGCTCATGTAGCCGCTTTTTCTgcg                  <  1:8844796/140‑1 (MQ=255)
                                                                                                                       gTTCTTTTTGGCGGGTTAGGGAAAAATGCCTGATAGCGCTTCGCTTATCAGGCCTACCCGCGCGACAACGTCATTTGTGGTTCGGCAAAATCTTCCAGAATGCCTCAATTAGCGGCTCATGTAGCCGCTTTTTCTGCGca                <  2:3597476/140‑1 (MQ=255)
                                                                                                                         tCTTTTTGGCGGGTTAGGGAAAAATGCCTGATAGCGCTTCGCTTATCAGGCCTACCCGCGCGACAACGTCATTTGTGGTTCGGCAAAATCTTCCAGAATGCCTCAATTAGCGGCTCATGTAGCCGCTTTTTCTGCGcaca              <  1:1431648/140‑1 (MQ=255)
                                                                                                                          cTTTTTGGCGGGTTAGGGAAAAATGCCTGATAGCGCTTCGCTTATCAGGCCTACCCGCGCGACAACGTCATTTGTGGTTCGGCAAAATCTTCCAGAATGCCTCAATTAGCGGCTCATGTAGCCGCTTTTTCTGCGcacac             >  2:7272093/1‑140 (MQ=255)
                                                                                                                          cTTTTTGGCGGGTTAGGGAAAAATGCCTGATAGCGCTTCGCTTATCAGGCCTACCCGCGCGACAACGTCATTTGTGGTTCGGCAAAATCTTCCAGAATGCCTCAATTAGCGGATCATGTAGCCGCTTTTTCTGCGcacac             <  2:1348009/140‑1 (MQ=255)
                                                                                                                             tttGGCGGGTTAGGGAAAAATGCCTGATAGCGCTTCGCTTATCAGGCCTACCCGCGCGACAACGTCATTTGTGGTTCGGCAAAATCTTCCAGAATGCCTCAATTAGCGGCTCATGTAGCCGCTTTTTCTGCGCACACACg          >  1:8338563/1‑140 (MQ=255)
                                                                                                                                 gCGGGTTAGGGAAAAATGCCTGATAGCGCTTCGCTTATCAGGCCTACCCGCGCGACAACGTCATTTGTgg                                                                            >  1:3364531/1‑70 (MQ=255)
                                                                                                                                 gCGGGTTAGGGAAAAATGCCTGATAGCGCTTCGCTTATCAGGCCTACCCGCGCGACAACGTCATTTGTgg                                                                            <  2:3364531/70‑1 (MQ=255)
                                                                                                                                     gTTAGGGAAAAATGCCTGATAGCGCTTCGCTTATCAGGCCTACCCGCGCGACAACGTCATTTGTGGTTCGGCAAAATCTTCCAGAATGCCTCAATTAGCGGCTCATGTAGCCGCTTTTTCTGCGCACACACGCCCAGCTc  >  1:2851649/1‑140 (MQ=255)
                                                                                                                                     gTTAGGGAAAAATGCCTGATAGCGCTTCGCTTATCAGGCCTACCCGCGCGAAAACGTCATTTGTGGTTCGGCAAAATCTTCAAGAATGCCTCAATTAGCGGATCATGTAGCCGCTTTTTCTGCGCACACACGCCCAGCTc  >  1:1144701/1‑140 (MQ=255)
                                                                                                                                         |                                                                                                                                       
ACCGACTACGGGCGCGTACTGGCGGCGTTCGAACTTGGCGACCATGAACCGGATTTCGAAACCCGGCTGAATGAGCTGGGCTACGATTGCCACGACGAAACCAATAACCCGGCGTTCAGGTTCTTTTTGGCGGGTTAAGGAAAAATGCCTGATAGCGCTTCGCTTATCAGGCCTACCCGCGCGACAACGTCATTTGTGGTTCGGCAAAATCTTCCAGAATGCCTCAATTAGCGGCTCATGTAGCCGCTTTTTCTGCGCACACACGCCCAGCTC  >  NZ_CP025268/3958657‑3958929

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑