BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP025268	3,978,525	C→T	L110F (CTT→TTT)	wzxE →	lipid III flippase WzxE

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP025268	3,978,525	0	C	T	98.8%	2193.6 / NA	601	L110F (CTT→TTT)	wzxE	lipid III flippase WzxE
Reads supporting (aligned to +/- strand): ref base C (0/0); major base T (361/231); minor base G (7/0); total (369/232)
Fisher's exact test for biased strand distribution p-value = 4.72e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 604 total aligned reads displayed.

AATACGTTGCCCAGTACCATGATAATCCGCAACAGCTGCGCCGCGTGGTCGGCACTTCATCAGCGATGGTACTTGGTTTCTCTACGCTGATGGCGCTGGTTTTTGTGCTGGCAGCTGCGCCAATCAGCCAGGGATTGCTTGGTAATACCGACTATCAGGGGCTGGTGCGTTTAGTGGCGCTGGTGCAAATGGGGATCGCCTGGGGCAACCTGTTACTGGCGCTGATGAAAGGCTTTCGCGATGCCGCAGGTAATGCGTTATCGCTGATTGTCGG  >  NZ_CP025268/3978388‑3978661

  tACGTTGCCCAGTACCATGATAATCCGCAACAGCTGCGCCGCGTGGTCGGCACTTCATCAGCGATGGTACTTGGTTTCTCTACGCTGATGGCGCTGGTTTTTGTGCTGGCAGCTGCGCCAATCAGCCAGGGATTGTTTgg                                                                                                                                      >  2:6202877/1‑140 (MQ=255)

  tACGTTGCCCAGTACCATGATAATCCGCAACAGCTGCGCCGCGTGGTCGGCACTTCATCAGCGATGGTACTTGGTTTCTCTACGCTGATGGCGCTGGTTTTTGTGCTGGCAGCTGCGCCAATCAGCCAGGGATTGTTTgg                                                                                                                                      >  1:9179836/1‑140 (MQ=255)

  tACGTTGCCCAGTACCATGATAATCCGCAACAGCTGCGCCGCGTGGTCGGCACTTCATCAGCGATGGTACTTGGTTTCTCTACGCTGATGGCGCTGGTTGTTGTGCTGGCAGCTGCGCCAATCAGCCAGGGATTGTTTgg                                                                                                                                      >  1:4729549/1‑140 (MQ=255)

    cGTTGCCCAGTACCATGATAATCCGCAACAGCTGCGCCGCGTGGTCGGCACTTCATCAGCGATGGTACTTGGTTTCTCTACGCTGATGGCGCTGGTTTTTGTGCTGGCAGCTGCGCCAATCAGCCAGGGATTGTTTGGTa                                                                                                                                    >  2:3628305/1‑140 (MQ=255)

    cGTTGCCCAGTACCATGATAATCCGCAACAGCTGCGCCGCGTGGTCGGCACTTCATCAGCGATGGTACTTGGTTTCTCTACGCTGATGGCGCTGGTTTTTGTGCTGGCAGCTGCGCCAATCAGCCAGGGATTGTTTGGTa                                                                                                                                    >  2:8202240/1‑140 (MQ=255)

    cGTTGCCCAGTACCATGATAATCCGCAACAGCTGCGCCGCGTGGTCGGCACTTCATCAGCGATGGTACTTGGTTTCTCTACGCTGATGGCGCTGGTTTTTGTGCTGGCAGCTGCGCCAATCAGCCAGGGATTGTTTGGTa                                                                                                                                    >  2:552985/1‑140 (MQ=255)

       tGCCCAGTACCATGATAATCCGCAACAGCTGCGCCGCGTGGTCGGCACTTCATCAGCGATGGTACTTGGTTTCTCTACGCTGATGGCGCTGGTTTTTGTGCTGGCAGCTGCGCCAATCAGCCAGGGATTGTTTGGTAATa                                                                                                                                 >  2:8913434/1‑140 (MQ=255)

       tGCCCAGTACCATGATAATCCGCAACAGCTGCGCCGCGTGGTCGGCACTTCATCAGCGATGGTACTTGGTTTCTCTACGCTGATGGCGCTGGTTTTTGTGCTGGCAGCTGCGCCAATCAGCCAGGGATTGTTTGGTAATa                                                                                                                                 >  1:4774897/1‑140 (MQ=255)

       tGCCCAGTACCATGATAATCCGCAACAGCTGCGCCGCGTGGTCGGCACTTCATCAGCGATGGTACTTGGTTTCTCTACGCTGATGGCGCTGGTTTTTGTGCTGGCAGCTGCGCCAATCAGCCAGGGATTGTTTGGTAATa                                                                                                                                 >  1:5036133/1‑140 (MQ=255)

       tGCCCAGTACCATGATAATCCGCAACAGCTGCGCCGCGTGGTCGGCACTTCATCAGCGATGGTACTTGGTTTCTCTACGCTGATGGCGCTGGTTTTTGTGCTGGCAGCTGCGCCAATCAGCCAGGGAGTGTTTGGTAATa                                                                                                                                 >  2:6102484/1‑140 (MQ=255)

        gCCCAGTACCATGATAATCCGCAACAGCTGCGCCGCGTGGTCGGCACTTCATCAGCGATGGTACTTGGTTTCTCTACGCTGATGGCGCTGGTTTTTGTGCTGGCAGCTGCGCCAATCAGCCAGGGATTGTTTGGTAATAc                                                                                                                                >  2:1982372/1‑140 (MQ=255)

        gCCCAGTACCATGATAATCCGCAACAGCTGCGCCGCGTGGTCGGCACTTCATCAGCGATGGGACTTGGTTTCTCTACGCTGATGGCGCTGGTTTTTGTGCTGGCAGCTGCGCCACTCAGCCAGGGATTGTTGGGGAATAc                                                                                                                                >  2:4088245/1‑140 (MQ=255)

          ccAGTACCACGATACTCCGCAACAGCTGCGCCGCGTGGACGGCACTTCATCAGCGATGCTACTTGGTTTCTCTCCGCTGATGGCGCTGGATTTTGTGCTGGCAGCTGCGCCAATCAGCCAGGGATTGTTTGGTAATACCg                                                                                                                              <  2:5806550/140‑1 (MQ=255)

           cAGTACCATGATAATCCGCAACAGCTGCGCCGCGTGGTCGGCACTTCATCAGCGATGGTACTTGGTTTCTCTACGCTGATGGCGCTGGTTTTTGTGCTGGCAGCTGCGCCAATCAGCCAGGGATTGTTTGGTAATACCGa                                                                                                                             <  2:6781491/140‑1 (MQ=255)

           cAGTACCATGATAATCCGCAACAGCTGCGCCGCGTGGTCGGCACTTCATCAGCGATGGTACTTGGTTTCTCTACGCTGATGGCGCTGGTTTTTGTGCTGGCAGCTGCGCCAATCAGCCAGGGATTGTTTGGTAATACCGa                                                                                                                             <  1:2963044/140‑1 (MQ=255)

           cAGTACCATGATAATCCGCAACAGCTGCGCCGCGTGGTCGGCACTTCATCAGCGATGGTACTTGGTTTCTCTACGCTGATGGCGCTGGTTTTTGTGCTGGCAGCTGCGCCAATCAGCCAGGGATTGTTTGGTAATACCGa                                                                                                                             <  1:8719076/140‑1 (MQ=255)

           cAGTACCATGATAATCCGCAACAGCTGCGCCGCGTGGTCGGCACTTCATCAGCGATGGTACTTGGTTTCTCTACGCTGATGGCGCTGGTTTTTGTGCTGGCAGCTGCGCCAATCAGCCAGGGATTGTTTGGTAATACCGa                                                                                                                             <  1:803723/140‑1 (MQ=255)

            aGTACCATGATAATCCGCAACAGCTGCGCCGCGTGGTCGGCACTTCATCAGCGATGGTACTTGGTTTCTCTACGCTGATGGCGCTGGTTTTTGTGCTGGCAGCTGCGCCAATCAGCCAGGGATTGTTTGGTAATACCGAc                                                                                                                            >  2:3035585/1‑140 (MQ=255)

             gTACCATGATAATCCGCAACAGCTGCGCCGCGTGGTCGGCACTTCATCAGCGATGGTACTTGGTTTCTCTACGCTGATGGCGCTGGTTTTTGTGCTGGCAGCTGCGCCAATCAGCCAGGGATTGTTTGGTAATACCGACt                                                                                                                           >  1:7800282/1‑140 (MQ=255)

              tACCATGATAATCCGCAACAGCTGCGCCGCGTGGTCGGCACTTCATCAGCGATGGTACTTGGTTTCTCTACGCTGATGGCGCTGGTTTTTGTGCTGGCAGCTGCGCCAATCAGCCAGGGATTGTTTGGTAATACCGACTa                                                                                                                          >  2:9023722/1‑140 (MQ=255)

              tACCATGATAATCCGCAACAGCTGCGCCGCGTGGTCGGCACTTCATCAGCGATGGTACTTGGTTTCTCTACGCTGATGGCGCTGGTTTTTGTGCTGGCAGCTGCGCCAATCAGCCAGGGATTGTTTGGTAATACCGACTa                                                                                                                          >  1:2333740/1‑140 (MQ=255)

              tACCATGATAATCCGCAACAGCTGCGCCGCGTGGTCGGCACTTCATCAGCGATGGTACTTGGTTTATCTACGCTGATGGCGCTGGTTTTTGTGCTGGCAGCTGCGCCAATCAGCCAGGGATTGTTTGGTAATACCGACTa                                                                                                                          >  1:3035098/1‑140 (MQ=255)

                 cATGATAATCCGCAACAGCTGCGCCGCGTGGTCGGCACTTCATCAGCGATGGTACTTGGTTTCTCTACGCTGATGGCGCTGGTTTTTGTGCTGGCAGCTGCGCCAATCAGCCAGGGATTGTTTGGTAATACCGACTATCa                                                                                                                       >  1:4247291/1‑140 (MQ=255)

                   tGATAATCCGCAACAGCTGCGCCGCGTGGTCGGCACTTCATCAGCGATGGTCCTTGGTTTCTCTACGCTGATGGCGCTGGTTTTTGTGCTGGCAGCTGCGCCAATCAGCCAGGGATTGTTTGGTAATACCGACTATCAgg                                                                                                                     >  2:3188571/1‑140 (MQ=255)

                   tGATAATCCGCAACAGCTGCGCCGCGTGGTCGGCACTTCATCAGCGATGGTACTTGGTTTCTCTACGCTGATGGCGCTGGTTTTTGTGCTGGCAGCTGCGCCAATCAGCCAGGGATTGTTTGGTAATACCGACTATCAgg                                                                                                                     >  2:6004624/1‑140 (MQ=255)

                   tGATAATCCGCAACAGCTGCGCCGCGTGGTCGGCACTTCATCAGCGATGGTACTTGGTTTCTCTACGCTGATGGCGCTGGTTTTTGTGCTGGCAGCTGCGCCAATCAGCCAGGGATTGTTTGGTAATACCGACTATCAgg                                                                                                                     >  1:3516454/1‑140 (MQ=255)

                   tGATAATCCGCAACAGCTGCGCCGCGTGGTCGGCACTTCATCAGCGATGGTACTTGGTTTCTCTACGCTGATGGCGCTGGTTTTTGTGCTGGCAGCTGCGCCAATCAGCCAGGGATTGTTTGGTAATACCGACTATCAgg                                                                                                                     >  2:2269518/1‑140 (MQ=255)

                   tGATAATCCGCAACAGCTGCGCCGCGTGGTCGGCACTTCATCAGCGATGGTACTTGGTTTCTCTACGCTGATGGCGCTGGTTTTTGTGCTGGCAGCTGCGCCAATCAGCCAGGGATTGTTTGGTAATACCGACTATCAgg                                                                                                                     >  1:1876352/1‑140 (MQ=255)

                   tGATAATCCGCAACAGCTGCGCCGCGTGGTCGGCACTTCATCAGCGATGGTACTTGGTTTCTCTACGCTGATGGCGCTGGGTTTTGTGCTGGCAGCTGCGCCAATCAGCCAGGGATTGTTTGGTAATACCGACTATCAgg                                                                                                                     >  1:5122110/1‑140 (MQ=255)

                   tGATAAACCGCAACAGCTGCGCCGCGTGGTCGGCACTTCATCAGCGATGGTACTTGGTTTCTCTACGCTGATGGCGCTGGTTTTTGTGCTGGCAGCTGCGCCAATCAGCCANGGAGTGTTTGGTAATACCGACTATCTgg                                                                                                                     >  1:5564055/1‑140 (MQ=255)

                        aTCCGCAACTGCTGCGCCGCGGGGTCGGCACTTCATCAGCGATGGTACTTTGTTTCTCTACGCTGATGGCGCTGGTTTTTGTGCTGGCAGCTGCGCCAATCAGCCGGGGCTTGGTTGGTATGTCCGTCTATCAGGGGCTg                                                                                                                >  2:8508241/1‑140 (MQ=255)

                        aTCCGCAACAGCTGCGCCGCGTGGTCGGCACTTCATCAGCGATGGTACTTGGTTTCTCTACGCTGATGGCGCTGGTTTTTGTGCTGGCAGCTGCGCCAATCAGCCAGGGATTGTTTGGTAATACCGACTATCAGGGGCTg                                                                                                                >  1:3964544/1‑140 (MQ=255)

                        aTCCGCAACAGCTGCGCCGCGTGGTCGGCACTTCATCAGCGATGGTACTTGGTTTCTCTACGCTGATGGCGCTGGTTTTTGTGCTGGCAGCTGCGCCAATCAGCCAGGGATTGTTTGGTAATACCGACTATCAGGGGCTg                                                                                                                >  2:7441928/1‑140 (MQ=255)

                        aTCCGCAACAGCTGCGCCGCGTGGTCGGCACTTCATCAGCGATGGTACTTGGTTTCTCTACGCTGATGGCGCTGGTTTTTGTGCTGGCAGCTGCGCCAATCAGCCAGGGAGTGTTTGGTAATACCGACTATCAGGTGCTg                                                                                                                >  2:2732699/1‑140 (MQ=255)

                        aTCCGCAACAGCTGCGCCGCGTGGTCGGCACTTCATCAGCGATGGTACTGGGTTTCTCTACGCTGATGGCGCTGGTGGTTGTGCGGGCAGCTGCGCCAATCAGCCAGGGAGTGTTTGGTAATACCGACTATCAGGGGCTg                                                                                                                >  2:1680010/1‑140 (MQ=255)

                        aTCCGCAACAGCGGCGCCGCGTGGTCGGCACTTCATCAGCGAGGGTACGGGGTGTCTCTACGCTGATGGCGCGGGTGTTTGTGCTGGCAGCTGCGCCAATCAGCCAGGGATTGTGTGGTACTACCGACGAGCAGGGGCTg                                                                                                                >  2:6885065/1‑140 (MQ=255)

                        aTCCGAAACATCAGCTCCGCGTGGTCGGCACATCATCAGCGATGTTACTTGGTATCTCTACGCTGAAGGCGCTGGTTTTTGAGCTGGAAGCTGCTCCAATCAGACAGGGATTGTTTGGTAATACCGACTATCAGCGGCTg                                                                                                                >  2:4528306/1‑140 (MQ=255)

                          ccGCAACAGCTGCGCCGCGTGGTCGGCACTTCATCAGCGATGGTACTTGGTTTCTCTACGCTGATGGCGCTGGTTTTTGTGCTGGCAGCTGCGCCAATCAGCCAGGGATTGTTTGGTAATACCGACTATCAGGGGCTGGt                                                                                                              <  1:9428411/140‑1 (MQ=255)

                          ccGCAACAGCTGCGCCGCGTGGTCGGCACTTCATCAGCGATGGTACTTGGTTTCTCTACGCTGATGGCGCTGGTTTTTGTGCTGGCAGCTGCGCCAATCAGCCAGGGAGTGTTTGGTAATACCGACTATCAGGGGCTGGt                                                                                                              >  2:6980512/1‑140 (MQ=255)

                          ccGCAACAGCTGCGCCGCGTGGACGGCACTTCATCAGCGATGGTACTTGGTTTCTCTCCGCTGATGGCGCTGGTTTTTGTGCTGGCAGCTGCGCCAATCAGCCAGGGATTGTTTGGTAATACCGACTATCAGGGGCTGGt                                                                                                              <  1:1748151/140‑1 (MQ=255)

                            gCAACAGCTGCGCCGCGTGGTCGGCACTTCATCAGCGATGGTACTTGGTTTCTCTACGCTGATGGCGCTGGTTTTTGTGCTGGCAGCTGCGCCAATCAGCCAGGGATTGTTTGGTAATACCGACTATCAGGGGCTGGTGc                                                                                                            <  1:8754087/140‑1 (MQ=255)

                             cAACAGCTGCGCCGCGTGGTCGGCACTTCATCAGCGATGGTACTTGGTTTCTCTACGCTGATGGCGCTGGTTTTTGTGCTGGCAGCTGCGCCAATCAGCCAGTGATTGTATGGTAATACAGACTATCAGGGGCTGGTGCg                                                                                                           >  2:1118373/1‑140 (MQ=255)

                             cAACAGCTGCGCCGCGTGGTCGGCACTTCATCAGCGATGGTACTTGGTTTCTCTACGCTGATGGCGCTGGTTTTTGTGCTGGCAGCTGCGCCAATCAGCCAGGGATTGTTTGGTAATACCGACTATCAGGGGCTGGTGCg                                                                                                           <  1:1035063/140‑1 (MQ=255)

                             cAACAGCTGCGCCGCGTGGTCGGCACTTCATCAGCGATGGTACTTGGTTTCTCTACGCTGATGGCGCTGGTTTTTGTGCTGGCAGCTGCGCCAATCAGCCAGGGATTGTTTGGTAATACCGACTATCAGGGGCTGGTGCg                                                                                                           >  1:7936449/1‑140 (MQ=255)

                              aaCAGCTGCGCCGCGTGGTCGGCACTTCATCAGCGATGGTACTTGGTTTCTCTACGCTGATGGCGCTGGTTTTTGTGCTGGCAGCTGCGCCAATCAGCCAGGGATTGTTTGGTAATACCGACTATCAGGGGCTGGTGCGt                                                                                                          >  1:9043520/1‑140 (MQ=255)

                                   cTGCGCCGCGTGGTCGGCACTTCATCAGCGATGGTACTTGGTTTCTCTACGCTGATGGCGCTGGTTTTTGTGCTGGCAGCTGCGCCAATCAGCCAGGGATTGTTTGGTAATACCGACTATCAGGGGCTGGTGCGTTTAGt                                                                                                     >  1:4390042/1‑140 (MQ=255)

                                    tGCGCCGCGTGGTCGGCACTTCATCAGCGATGGTACTTGGTTTCTCTACGCTGATGGCGCTGGTTTTTGTGCTGGCAGCTGCGCCAATCAGCCAGGGATTGTTTGGTAATACCGACTATCAGGGGCTGGTGCGTTTAGTg                                                                                                    >  1:4831555/1‑140 (MQ=255)

                                     gcgcCGCGTGGTCGGCACTTCATCAGCGATGGTACTTGGTTTCTCTACGCTGATGGCGCTGGTTTTTGTGCTGGCAGCTGCGCCAATCAGCCAGGGATTGTTTGGTAATACCGACTATCAGGGGCTGGTGCGTTTAGTgg                                                                                                   >  1:2673551/1‑140 (MQ=255)

                                        ccgcGTGGTCGGCACTTCATCAGCGATGGTACTTGGTTTCTCTACGCTGATGGCGCTGGTTTTTGTGCTGGCAGCTGCGCCAATCAGCCAGGGATTGTTTGGTAATACCGACTATCAGGGGCTGGTGCGTTTAGTGgcgc                                                                                                <  2:4835461/140‑1 (MQ=255)

                                        ccgcGTGGTCGGCACTTCATCAGCGATGGTACTTGGTTTCTCTACGCTGATGGCGCTGGTTTTTGTGCTGGCAGCTGCGCCAATCAGCCAGGGATTGTTTGGTAATACCGACTATCAGGGGCTGGTGCGTTTAGTGgcgc                                                                                                <  2:393088/140‑1 (MQ=255)

                                        cagctttgTCGGCACTTGATCTGCGATGGTACTTGGTTTCTCTTCGCTGATGGCGCTGGTTTTTTTGCTGGCTGCTGCTCCATTAACCCAGGGATTGTTTGGTAATACCGACTATCAGGGGCTGGTGCGTTTAGTGgcgc                                                                                                <  2:7711381/133‑1 (MQ=255)

                                           cgTGGTCGGCACTTCATCAGCGATGGTACTTGGTTTCTCTACGCTGATGGCGCTGGTTTTTGTGCTGGCAGCTGCGCCAATCAGCCAGGGATTGTTTGGTAATACCGACTATCAGGGGCTGGTGCGTTTAGTGGCGCTgg                                                                                             <  1:8546815/140‑1 (MQ=255)

                                             tGGTCGGCACTTCATCAGCGATGGTACTTGGTTTCTCTACGCTGATGGCGCGGGTGTTTGTGCTGGCAGCTGCGCCAATCAGCCAGGGAGTGTGTGGTAATACCGACTATCAGGGGCGGGTGCGTGTAGTGGCGCTGGTg                                                                                           >  1:7637278/1‑140 (MQ=255)

                                             gggacGGCACTTCATCAGCGATGGTACTTGGTTTCTCTACGCTGATGGCGCTGGTTTTTGTGCTGGCAGCTGCGCCAATCAGCCAGGGTTTGTTTGGTAATACCGACTATCAGGGGCTGGTGCGTTTAGTGGCGCTGGTg                                                                                           <  1:815833/136‑1 (MQ=255)

                                                tCGGCACTTCATCAGCGATGGTACTTGGTTTCTCTACGCTGATGGCGCTGGTTTTTGTGCTGGCAGCTGCGCCAATCAGCCAGGGATTGTTTGGTAATACCGACTATCAGGGGCTGGTGCGTTTAGTGGCGCTGGTGCaa                                                                                        >  1:8361995/1‑140 (MQ=255)

                                                     aCTTCATCAGCGATGGTACTTGGTTTCTCTACGCTGATGGCGCTGGTTTTTGTGCTGGCAGCTGCGCCAATCAGCCAGGGATTGTTTGGTAATACCGACTATCAGGGGCTGGTGCGTTTAGTGGCGCTGGTGCAAATggg                                                                                   >  2:3366783/1‑140 (MQ=255)

                                                      cTTCATCAGCGATGGTACTTGGTTTCTCTACGCTGATGGCGCTGGTTTTTGTGCTGGCAGCTGCGCCAATCAGCCAGGGATTGTTTGGTAATACCGACTATCAGGGGCTGGTGCGTTTAGTGGCGCTGGTGCAAATgggg                                                                                  <  1:5951982/140‑1 (MQ=255)

                                                       ttCATCAGCGATGGTACTTGGTTTCTCTACGCTGATGGCGCTGGTTTTTGTGCTGGCAGCTGCGCCAATCAGCCAGGGATTGTTTGGTAATACCGACTATCAGGGGCTGGTGCGTTTAGTGGCGCTGGTGCAAATGGGGa                                                                                 <  1:1502492/140‑1 (MQ=255)

                                                       ttCATCAGCGATGGTACTTGGTTTCTCTACGCTGATGGCGCTGGTTTTTGTGCTGGCAGCTGCGCCAATCAGCCAGGGATTGTTGGGTAATACCGACTATCAGGGGCTGGTGCGTTTAGTGGCGCTGGTGCAAATGGGGa                                                                                 <  2:7534549/140‑1 (MQ=255)

                                                       ttCATCAGCGATGGTACTTGGTTTCTCTACGCGGCTGGCGCTGGTTTTTGTGCTGGCAGCTGCGCCAATCAGCCAGGGATTGTTTGGTAATACCGAGTATCAGGGGCTGGTGCGTTTTGTGGCGCTGGTGCAAATGGGGa                                                                                 <  2:2547246/140‑1 (MQ=255)

                                                       ttCATCAGCGATGGTACTTGGTTTCTCTACGCGGATGGCGCTGGATTGTGTGCTGGCAGCTGCGCCACTCAGCCAGGGATTGTTTGGTAATACCGACTATCAGGGGCTGGTGCGTTTAGTGGCGCTGGTGCAAATGGGGa                                                                                 <  1:5662964/140‑1 (MQ=255)

                                                       ttCATCAGCGATGGTACTTGGAGTCTATTCGCTGATGGCGCTGGATATGGGGCTGGCAGCTGCGCCAAACAGCCAGGGATTGTTTGGGAAGACCGTCTATCAGGGGCTTGTGCTTTTAGAGGCGCTGGTGCAAATGGGGa                                                                                 <  2:3554221/140‑1 (MQ=255)

                                                         catcaGCGATGGTACTTGGTTTCTCTACGCTGATGGCGCTGGTTTTTGTGCTGGCAGCTGCGCCAATCAGCCAGGGATTGTTTGGTAATACCGACTATCAGGGGCTGGTGCGTTTAGTGGCGCTGGTGCAAATGGGGATc                                                                               >  2:8769497/1‑140 (MQ=255)

                                                           tcaGCGATGGTACTTGGTTTCTCTACGCTGATGGCGCTGGTTTTTGTGCTGGCAGCTGCGCCAATCAGCCAGGGATTGTTTGGTAATACCGACTATCAGGGGCTGGTGCGTTTAGTGGCGCTGGTTCAAATGGGGATCGc                                                                             <  2:912417/140‑1 (MQ=255)

                                                           tcaGCGATGGTACTTGGTTTCTCTACGCTGATGGCGCTGGTTTTTGTGCTGGCAGCTGCGCCAATCAGCCAGGGATTGTTTGGTAATACCGACTATCAGGGGCTGGTGCGTTTAGTGGCGCTGGTGCAAATGGGGATCGc                                                                             <  1:7474520/140‑1 (MQ=255)

                                                           tcaGCGAGGGTACTTGGTGTCTCTACGCTGATGGCGCTGGATTTTGTGCTGGCAGCTGCGCCAATCAGCCAGGGATTGTTTGGTAATACCGACTATCAGGGGCTGGTGCGTTTAGTGGCGCTGGTGCAAATGGGGATCGc                                                                             <  1:9003681/140‑1 (MQ=255)

                                                            caGCGATGGTACTTGGTTTCTCTACGCTGATGGCGCTGGTTTTTGTGCTGGCAGCTGCGCCAATCAGCCAGGGATTGTTTGGTAATACCGACTATCAGGGGCTGGTGCGTTTAGTGGCGCTGGTGCAAATGGGGATCGcc                                                                            <  1:3272289/140‑1 (MQ=255)

                                                             aGCGATGGTACTTGGTTTCTCTACGCTGATGGCGCTGGTTTTTGTGCTGGCAGCTGCGCCAATCAGCCAGGGATTGTTTGGTAATACCGACTATCAGGGGCTGGTGCGTTTAGTGGCGCTGGTGCAAATGGGGATCGCCt                                                                           <  2:7039375/140‑1 (MQ=255)

                                                                 aTGGTACTTGGTTTCTCTCCGCTGATGGCGCTGGTTTTTGTGCTGGCAGCGGCGCCAATCAGCCAGGGATTGTTTGGTAAGACCGACTATCAGGGGCGGGTGCGTTTAGTGGCGCTGGTGCAAATGGGGATCGCCTgggg                                                                       <  1:2559120/140‑1 (MQ=255)

                                                                 aTGGTACTTGGTTTCTCTACGCTGATGGCGCTGGTTTTTGTGCTGGCAGCTGCGCCAATCAGCCAGGGATTGTTTGGTAATACCGACTATCAGGGGCTGGTGCGTTTAGTGGCGCTGGTGCAAATGGGGATCGCCTgggg                                                                       >  1:7044664/1‑140 (MQ=255)

                                                                 aTGGTACTTGGTTTCTCTACGCTGATGGCGCTGGTTTTTGTGCTGGCAGCTGCGCCAATCAGCCAGGGATTGTTTGGTAATACCGACTATCAGGGGCTGGTGCGTTGAGTGGCGCTGGTGCAAATGGGGATCGCCTgggg                                                                       >  1:3897577/1‑140 (MQ=255)

                                                                  tGGTACTTGGTTTCTCTACGCTGATGGCGCTGGTTTTTGTGCTGGCAGCTGCGCCAATCAGCCAGGGATTGTTTGGTAATACCGACTATCAGGGGCTGGTGCGTTTAGTGGCGCTGGTGCAAATGGGGATCGCCTGGGGc                                                                      <  1:6176362/140‑1 (MQ=255)

                                                                       cTTGGTTTCTCTACGCTGATGGCGCTGGTTTTTGTGCTGGCAGCTGCGCCAATCAGCCAGGGATTGTTTGGTAATACCGACTATCAGGGGCTGGTGCGTTTAGTGGCGCTGGTGCAAATGGGGATCGCCTGGGGCAACCt                                                                 >  1:181461/1‑140 (MQ=255)

                                                                             ttCTCTACGCTGATGGCGCTGGTTTTGGTGCTGGCAGGTGCGCCAATCAGCAAGGGATTGTTTGGTAATACCGACTTTCTGGGGCTGGTGCGTTTTGTGGCGCTGGTGCTATGGGGGATCGCCTGGGGCAACCTGTTagg                                                           >  2:4671714/1‑138 (MQ=255)

                                                                              tctcTACGCTGATGGCGCTGGTTTTTGTGCTGGCAGCTGCGCCAATCAGCCAGGGCTTGTTTGGTAATACCGACTATCAGGGGCTGGTGCGTTGAGTGGCGCTGGTGCAAATGGGGATCGCCTGGGGCACCCAGTTACTg                                                          >  2:7190632/1‑140 (MQ=255)

                                                                              tctcTACGCTGATGGCGCTGGTTTTTGTGCTGGCAGCTGCGCCAATCAGCCAGGGATTGTTTGGTAATACCGACTATCAGGGGCTGGTGCGTTTAGTGGCGCTGGTGCAAATGGGGATCGCCTGGGGCAACCTGTTACTg                                                          >  2:6462269/1‑140 (MQ=255)

                                                                               ctctACGCTGATGGCGCTGGTTTTTGTGCTGGCAGCTGCGCCAATCAGCCAGGGATTGTTTGGTAATACCGACTATCAGGGGCTGGTGCGTTTAGTGGCGCTGGTGCAAATGGGGATCGCCTGGGGCAACCTGTTACTgg                                                         >  1:2438838/1‑140 (MQ=255)

                                                                               ctctACGCTGATGGCGCTGGTTTTTGTGCTGGCAGCTGCGCCAATCAGCCAGGGATTGTTTGGTAATACCGACTATCAGGGGCTGGTGCGTTTAGTGGCGCTGGTGCAAATGGGGATCGCCTGGGGCAACCTGTTACTgg                                                         >  1:2167355/1‑140 (MQ=255)

                                                                                tctACGCTGATGGCGCTGGTTTATGTGCTGGCAGCTGCGCCAATCAGCCAGGGATTGTTTGGTAATACCGACTATCAGGGGCTGGTGCGTTTAGTGGCGCTGGTGCAAATGGGGATCGCCTGGGGCAACCTGTTACTGgc                                                        >  2:2394378/1‑140 (MQ=255)

                                                                                  tACGCTGATGGCGCTGGTTTTTGTGCTGGCAGCTGCGCCAATCAGCCAGGAATTGTTTGGTAATACCGACTATCAGGGGCTGGTGCGTTTAGTGGCGCTGGTGCAAATGGGGATCGCCTGGGGCAACCTGTTACTGgcgc                                                      <  2:8410300/140‑1 (MQ=255)

                                                                                  tACGCTGATGGCGCTGGATTTTGTGCTGGCAGCTGCGCCAATCAGCCAGGGATTGTTTGGTAATACCGACTATCAGGGGCTGGTGCGTTTAGTGGCGCTGGTGCAAATGGGGATCGCCTGGGGCAACCTGTTACTGgcgc                                                      <  2:4906118/140‑1 (MQ=255)

                                                                                         aTGGCGCTGGTTTTTGTGCTGGCAGCTGCGCCAATCAGCCAGGGATTGTTTGGTAATACCGACTATCAGGGGCTGGTGCGTTTAGTGGCGCTGGTGCAAATGGGGATCGCCTGGGGCAACCTGTTACTGGCGCTGATGaa                                               >  2:9384802/1‑140 (MQ=255)

                                                                                         aTGGCGCTGGTTTTTGTGCTGGCAGCTGCGCCAATCAGCCAGGGATTGTTTGGTAATACCGACTATCAGGGGCTGGTGCGTTTAGTGGCGCTGGTGCAAATGGGGATCGCCTGGGGCAACCTGTTACTGGCGCTGATGaa                                               >  2:2958108/1‑140 (MQ=255)

                                                                                         aTGGCGCTGGTTTTTGTGCTGGCAGCTGCGCCAATCAGCCAGGGATTGTTTGGTAATACCGACTATCAGGGGCTGGTGCGTTTAGTGGCGCTGGTGCAAATGGGGATCGCCTGGGGCAACCTGTTACTGGCGCTGATGaa                                               >  1:6836296/1‑140 (MQ=255)

                                                                                         aTGGCGCTGGTTTTTGTGCTGGCAGCTGCGCCAATCAGCCAGGGATTGTTTGGTAATACCGACTATCAGGGGCTGGTGCGTTGAGTGGCGCTGGTGCAAATGGGGATCGCCTGGGGCAAACTGTTACTGGCGCTGATGaa                                               >  2:1317996/1‑140 (MQ=255)

                                                                                         aTGGCGCTGGTTTTTGTGCTGGCAGCTGCGCCAATCAGCCAGGGATTGTTTGGTAATACCGACTATCAGGGGCAGGTGCGTGTAGTGGCGCTGGTGCAAATGGGGATCGCCTGGGGCAACCTGTTACTGGCGCTGATGaa                                               >  1:3762266/1‑140 (MQ=255)

                                                                                              gcTGGTTTTTGTGCTGGCAGCTGCGCCAATCAGCCAGGGATTGTTTGGTAATACCGACTATCAGGGGCTGGTGCGTTTAGTGGCGCTGGTGCAAATGGGGATCGCCTGGGGCa                                                                     <  1:405830/113‑1 (MQ=255)

                                                                                                    ttttGTGCTGGCAGCTGCGCCAATCAGCCAGGGATTGTTTGGTAATACCGACTATCAGGGGCTGGTGCGTTTAGTGGCGCTGGTGCAAATGGGGATCGCCTGGGGCAACCTGTTACTGGCGCTGATGAAAGGCTTTcgcg                                    <  1:6278330/140‑1 (MQ=255)

                                                                                                          gCTGGCAGCTGCGCCAATCAGCCAGGGATTGTTTGGTAATACCGACTATCAGGGGCTGGTGCGTTTAGTGGCGCTGGTGCAAATGGGGATCGCCTGGGGCAACCTGTTACTGGCGCTGATGAAAGGCTTTCGCGATGCCg                              <  1:6466102/140‑1 (MQ=255)

                                                                                                          gCTGGCAGCTGCGCCAATCAGCCAGGGATTGTTTGGTAATACCGACTATCAGGGGCTGGTGCGTTTAGTGGCGCTGGTGCAAATGGGGATCGCCTGGGGCAACCTGTTACTGGCGCTGATGAAAGGCTTTCGCGATGCCg                              <  1:1551283/140‑1 (MQ=255)

                                                                                                            tGGCAGCTGCGCCAATCAGCCAGGGATTGTTTGGTAATACCGACTATCAGGGGCTGGTGCGTTTAGTGGCGCTGGTGCAAATGGGGATCGCCTGGGGCAACCTGTTACTGGCGCTGATGAAAGGCTTTCGCGATGCCGCa                            >  2:5244085/1‑140 (MQ=255)

                                                                                                            tGGCAGCTGCGCCAATCAGCCAGGGATTGTTTGGTAATACCGACTATCAGGGGCTGGTGCGTTTAGTGGCGCTGGTGCAAATGGGGATCGCCTGGGGCAACCTGTTACTGGCGCTGATGAAAGGCTTTCGCGATGCCGCa                            >  2:1450786/1‑140 (MQ=255)

                                                                                                              gCAGCTGCGCCAATCAGCCAGGGATTGTTTGGTAATACCGACTATCAGGGGCTGGTGCGTTTAGTGGCGCTGGTGCAAATGGGGATCGCCTGGGGCAACCTGTTACTGGCGCTGATGAAAGGCTTTCGCGATGCCGCAgg                          <  1:1102545/140‑1 (MQ=255)

                                                                                                                     cgcCAATCAGCCAGGGATTGTTTGGTAATACCGACTATCAGGGGCTGGTGCGTTTAGTGGCGCTGGTGCAAGTGGGGATCGCCTGGGGCAACCTGTTACTGGCGCTGATGAAAGGCTTTCGCGATGCCGCAGGGAATGCg                   >  1:7323017/1‑140 (MQ=255)

                                                                                                                     cgcCAATCAGCCAGGGATTGTTTGGTAATACCGACTATCAGGGGCTGGTGCGTTTAGTGGCGCTGGTGCAAATGGGGATCGCCTGGGGCAACCTGTTACTGGCGCTGATGAAAGGCTTTCGCGATGCCGCAGGTAATGCg                   <  2:5002915/140‑1 (MQ=255)

                                                                                                                        cAATCAGCCAGGGATTGTTTGGTAATACCGACTATCAGGGGCTGGTGCGTTTAGTGGCGCTGGTGCAAATGGGGATCGCCTGGGGCAACCTGTTACTGGCGCTGATGAAAGGCTTTCGCGATGCCGCAGGTAATGCGTTa                >  1:2236222/1‑140 (MQ=255)

                                                                                                                            cAGCCAGGGATTGTTTGGTAATACCGACTATCAGGGGCTGGTGCGTTTAGTGGCGCTGGTGCAAATGGGGATCGCCTGGGGCAACCTGTTACTGGCGCTGATGAAAGGCTTTCGCGATGCCGCAGGTAATGCGTTATCGc            <  2:7936449/140‑1 (MQ=255)

                                                                                                                                  gggATTGTTTGGTAATACCGACTATCAGGGGCTGGTGCGTTTAGTGGCGCTGGTGCAAATGGGGATCGCCTGGGGCAACCTGTTACTGGCGCTGATGAAAGGCTTTCGCGATGCCGCAGGTAATGCGTTATCGCTGATTg      >  1:1333661/1‑140 (MQ=255)

                                                                                                                                    gATTGTTTGGTAATACCGACTATCAGGGGCTGGTGCGTTTAGTGGCGCTGGTGCAAATGGGGATCGCCTGGGGCAACCTGTTACTGGCGCTGATGAAAGGCTTTCGCGATGCCGCAGGTAATGCGTTATCGCTGATTGTc    >  1:5811709/1‑140 (MQ=255)

                                                                                                                                     aTTGTTTGGTAATACCGACTATCAGGGGCTGGTGCGTTTAGTGGCGCTGGTGCAAATGGGGATCGCCTGGGGCAACCTGTTACTGGCGCTGATGAAAGGCTTTCGCGATGCCGCAGGTAATGCGTTATCGCTGATTGTCg   >  1:5254131/1‑140 (MQ=255)

AATACGTTGCCCAGTACCATGATAATCCGCAACAGCTGCGCCGCGTGGTCGGCACTTCATCAGCGATGGTACTTGGTTTCTCTACGCTGATGGCGCTGGTTTTTGTGCTGGCAGCTGCGCCAATCAGCCAGGGATTGCTTGGTAATACCGACTATCAGGGGCTGGTGCGTTTAGTGGCGCTGGTGCAAATGGGGATCGCCTGGGGCAACCTGTTACTGGCGCTGATGAAAGGCTTTCGCGATGCCGCAGGTAATGCGTTATCGCTGATTGTCGG  >  NZ_CP025268/3978388‑3978661

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`