Predicted mutation
evidence seq id position mutation annotation gene description
RA NZ_CP025268 4,529,477 G→A P140L (CCG→CTG)  sgcA ← SgcA family PTS sugar transporter subunit IIA

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0252684,529,4770GA99.7% 2242.0 / NA 582P140L (CCG→CTG) sgcASgcA family PTS sugar transporter subunit IIA
Reads supporting (aligned to +/- strand):  ref base G (0/0);  major base A (331/249);  minor base C (0/2);  total (331/251)
Fisher's exact test for biased strand distribution p-value = 1.86e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 582 total aligned reads displayed.

CTGCTGTCCTCAATATCCAGATGCAGGCTCCCAAAATCCAGATTGTCCAATGCCGTCAGCTCACGACCATAGTGCAGAGGATTTGCGCTGGCGAGAGAAGGGTGCAGGATCATAAGCGCCTCCGTTAACCTGATACCGGCGCAAACAGTGCATCGTCTGTGCTGGCATTTCCCACTGCCGTTAAGCGTTGTGGCGAATCAATAAACTGGCTGATGCGCTGAATGGTCAAAATATGTGCGTTGGCATCCGTGGCGCTGACGCAGAGAAGCAGCCA  >  NZ_CP025268/4529340‑4529613
                                                                                                                                         |                                                                                                                                        
ctgctgTCCTCAATATCCAGATGCAGGCTCCCAAAATCCAGATTGTCCAAGGCCGTCAGCTCACGACCAGAGTGCAGAGGATTTGCGCTGGCGAGAGAAGGGTGCAGGATCATAAGCGCCTCCGTTATCATGATACCAgc                                                                                                                                        >  1:8633902/1‑140 (MQ=255)
 tgctgTCCTCAATATCCAGATGCAGGCTCCCAAAATCCAGATTGTCCAATGCCGTCAGCTCACGACCATAGTGCAGAGGATTTGCGCTGGCGAGAGAAGGGTGCAGGATCATAAGCGCCTCCGTTAACCTGATACCAgcg                                                                                                                                       >  1:7086329/1‑140 (MQ=255)
 tgctgTCCTCAATATCCAGATGCAGGCTCCCAAAATCCAGATTGTCCAATGCCGTCAGCTCACGACCATAGTGCAGAGGATTTGCGCTGGCGAGAGAAGGGTGCAGGATCATAAGCGCCTCCGTTAACCTGATACCAgcg                                                                                                                                       >  2:1543996/1‑140 (MQ=255)
 tgctgTCCTCAATATCCAGATGCAGGCTCCCAAAATCCAGATTGTCCAATGCCGTCAGCTCACGACCATAGTGCAGAGGATTTGCGCTGGCGAGAGAAGGGTGCAGGATCATAAGCGCCTCCGTTAACCTGATACCAgcg                                                                                                                                       >  1:2704409/1‑140 (MQ=255)
  gctgTCCTCACTATCCAGATGCAGGCTCCCATAATCCAGATTGTCCAATGCCGTCAGCTCACGACCAAAGTGCAGAGGATTTGCGCTGGCGAGAGAAGGGTGCAGGATCATAAGCGCCTCCGTTAACCTGATACCAgcgc                                                                                                                                      <  1:7979373/140‑1 (MQ=255)
  gctgTCCTCAATATCCAGATGCAGGCTCCCAAAATCCAGATTGTCCAATGCCGTCAGCTCACGACCATAGTGCAGAGGATTTGCGCTGGCGAGAGAAGGGTGCAGGATCATAAGCGCCTCCGTTAACCTGATACCAgcgc                                                                                                                                      <  1:5774388/140‑1 (MQ=255)
      tCCTCAATATCCAGATGCAGGCTCCCAAAATCCAGATTGTCCAATGCCGTCAGCTCACGACCATAGTGCAGAGGATTTGCGCTGGCGAGAGAAGGGTGCAGGATCATAAGCGCCTCCGTTAACCTGATACCAGCGCAAAc                                                                                                                                  <  2:2647914/140‑1 (MQ=255)
           aaTATCCAGATGCAGGCTCCCAAAATCCAGATTGTCCAATGCCGTCAGCTCACGACCATAGTGCAGAGGATTTGCGCTGGCGAGAGAAGGGTGCAGGATCATAAGCGCCTCCGTTAACCTGATACCAGCGCAAACAGTGc                                                                                                                             <  2:9232258/140‑1 (MQ=255)
            atatCCAGATGCAGGCTCCCAAAATCCAGATTGTCCAATGCCGTCAGCTCACGACCATAGTGCAGAGGATTTGCGCTGGCGAGAGAAGGGTGCAGGATCATAAGCGCCTCCGTTAACCTGATACCAGCGCAAACAGTGCa                                                                                                                            <  1:6794090/140‑1 (MQ=255)
            atatCCAGATGCAGGCTCCCAAAATCCAGATTGTCCAATGCCGTCAGCTCACGACCATAGTGCAGAGGATTTGCGCTGGCGAGAGAAGGGTGCAGGATCATAAGCGCCTCCGTTAACCTGATACCAGCGCAAACAGTGCa                                                                                                                            <  1:1455704/140‑1 (MQ=255)
                ccAGATGCAGGCTCCCAAAATCCAGATTGTCCAATGCCGTCAGCTCACGACCATAGTGCAGAGGATTTGCGCTGGCGAGAGAAGGGTGCAGGATCATAAGCGCCTCCGTTAACCTGATACCAGCGCAAACAGTGCATCGt                                                                                                                        >  2:5278503/1‑140 (MQ=255)
                ccAGATGCAGGCTCCCAAAATCCAGATTGTCCAATGCCGTCAGCTCACGACCATAGTGCAGAGGATTTGCGCTGGCGAGAGAAGGGTGCAGGATCATAAGCGCCTCCGTTAACCTGATACCAGCGCAAACAGTGCATCGt                                                                                                                        >  2:8737533/1‑140 (MQ=255)
                ccAGATGCAGGCTCCCAAAATCCAGANTGTCCAAGGCCGTCAGCTCACGACCATAGTGCAGAGGATTTGCGCTGGCGAGAGAAGGGTGCAGGATCATAAGCGCCTCCGTTAACCTGATACCAGCGCAAACAGTGCATCGt                                                                                                                        <  1:6351095/140‑1 (MQ=255)
                ccAGAGGCAGGCTCCCAAACTCCAGATTGTCCAAGGCCGTCAGCTCACGACCAGAGTGCAGAGGATTTGCGCGGGCGAGAGAAGGGTGCAGGATCATAAGCGCCTCCGTTAACCTGATACCAGCGCAAACAGTGCATCGt                                                                                                                        <  1:4348300/140‑1 (MQ=255)
                 cAGATGCAGGCTCCCAAAATCCAGATTGTCCAATGCCGTCAGCTCACGACCATAGTGCAGAGGATTTGCGCTGGCGAGAGAAGGGTGCAGGATCATAAGTGCCTCCGTTAACCTGATACCAGCGCAAACAGTGCATCGTc                                                                                                                       <  2:8671611/140‑1 (MQ=255)
                 cAGATGCAGGCTCCCAAAATCCAGAGTGTCCACTGCCGTCAGCTCACGACCATAGTGCAGAGGATTTGCGCTGGAGAGAGAAGGGTGCAGGATCATAATCGCCTCCGTTAACCTGATACCAGCGCAAACAGTGCATCGTc                                                                                                                       <  1:4596090/140‑1 (MQ=255)
                  aGATGCAGGCTCCCAAAATCCAGATTGTCCAATGCCGTCAGCTCACGACCATAGTGCAGAGGATTTGCGCTGGCGAGAGAAGGGTGCAGGATCATAATCGCCTCCGTTAACCTGATACCAGCGCAAACAGTGCATCGTca                                                                                                                      >  2:8063703/1‑139 (MQ=255)
                       cAGGCTCCCAAAATCCAGATTGTCCAATGCCGTCAGCTCACGACCATAGTGCAGAGGATTTGCGCTGGCGAGAGAAGGGTGCAGGATCATAAGCGCCTCCGTTAACCTGATACCAGCGCAAACAGTGCATCGTCTGTGCt                                                                                                                 >  2:6651617/1‑140 (MQ=255)
                       cAGGCTCCCAAAATCCAGATTGTCCAATGCCGTCAGCTCACGACCATAGTGCAGAGGATTTGCGCTGGCGAGAGAAGGGTGCAGGATCATAAGCGCCTCAGTTAACCTGATACCAGCGGAAACAGTGCATCGTCTGTGCt                                                                                                                 >  2:6793331/1‑140 (MQ=255)
                         ggCTCCCAAAATCCAGATTGTCCAATGCCGTCAGCTCACGACCATAGTGCAGAGGATTTGCGCTGGCGAGAGAAGGGTGCAGGATCATAAGCGCCTCCGTTAACCTGATACCAGCGCAAACAGTGCATCGTCTGTGCTgg                                                                                                               >  2:1301852/1‑140 (MQ=255)
                               cAAAATCCAGATTGTCCAATGCCGACAGCTCACGACCATAGTGCAGAGGATTTGCGCTGGCGAGAGAAGGTTGCAGGATCATAAGCGCCTCCGTTAACCTGATACCAGCGCAAACAGTGCATCGTCTGTGCTGGCATTTc                                                                                                         >  1:8209636/1‑140 (MQ=255)
                                  aaTCCAGATTGTCCAATGCCGTCAGCTCACGACCATAGTGCAGAGGATTTGCGCTGGCGAGAGAAGGGTGCAGGATCATAAGCGCCTCCGTTAACCTGATACCAGCGCAAACAGTGCATCGTCTGTGCTGGCATTTCCCa                                                                                                      <  2:481403/140‑1 (MQ=255)
                                   aTCCAGATTGTCCAATGCCGTCAGCTCACGACCATAGTGCAGAGGATTTGCGCTGGCGAGAGAAGGGTGCAGGATCATAAGCGCCTCCGTTAACCTGATACCAGCGCAAACAGTGCATCGTCTGTGCTGGCATTTCCCAc                                                                                                     <  2:5084531/140‑1 (MQ=255)
                                    tCCAGATTGTCCAATGCCGTCAGCTCACGACCATAGTGCAGAGGATTTGCGCTGGCGAGAGAAGGGTGCAGGATCATAAGCGCCTCCGTTAACCTGATACCAGCGCAAACAGTGCATCGTCTGTGCTGGCATTTCCCACt                                                                                                    >  2:4910310/1‑140 (MQ=255)
                                     ccAGATTGTCCAATGCCGTCAGCTCACGACCATAGTGCAGAGGATTTGCGCTGGCGAGAGAAGGGTGCAGGATCATAAGCGCCTCCGTTAACCTGATACCAGCGCAAACAGTGCATCGTCTGTGCTGGCATTTCCCACTg                                                                                                   <  1:8846017/140‑1 (MQ=255)
                                             tCCAATGCCGTCAGCTCACGACCATAGTGCAGAGGATTTGCGCTGGCGAGAGAAGGGTGCAGGATCATAAGCGCCTCCGTTAACCTGATACCAGCGCAAACAGTGCATCGTCTGTGCTGGCATTTCCCACTGCCGTTAAg                                                                                           >  2:3847713/1‑140 (MQ=255)
                                              ccAATGCCGTCAGCTCACGACCATAGTGCAGAGGATTTGCGCTGGCGAGAGAAGGGTGCAGGATCATAAGCGCCTCCGTTAACCTGATACCAGCGCAAACAGTGCATCGTCTGTGCTGGCATTTCCCACTGCCGTTAAGc                                                                                          >  2:2776581/1‑140 (MQ=255)
                                                  tGCCGTCAGCTCACGACCATAGTGCAGAGGATTTGCGCTGGCGAGAGAAGGGTGCAGGATCATAAGCGCCTCCGTTAACCTGATACCAGCGCAAACAGTGCATCGTCTGTGCTGGCATTTCCCACTGCCGTTAAGCGTtg                                                                                      >  2:3504787/1‑140 (MQ=255)
                                                     cGTCAGCTCACGACCATAGTGCAGAGGATTTGCGCTGGCGAGAGAAGGGTGCAGGATCATAAGCGCCTCCGTTAACCTGATACCAGCGCAAACAGTGCATCGTCTGTGCTGGCATTTCCCACTGCCGTTAAGCGTTGTgg                                                                                   <  1:2421319/140‑1 (MQ=255)
                                                     cGTCAGCTCACGACCATAGTGCAGAGGATTTGCGCTGGCGAGAGAAGGGTGCAGGATCATAAGCGCCTCCGTTAACCTGATACCAGCGCAAACAGTGCATCGTCTGTGCTGGCATTTCCCACTGCCGTTAAGCGTTGTgg                                                                                   <  1:4763458/140‑1 (MQ=255)
                                                       tCAGCTCACGACCATAGTGCAGAGGATTTGCGCTGGCGAGAGATGGGTGCAGGATCATAAGCGCCTCCGTTAACCTGCTACCAGCGCAAAAAGTGCATCGTCTGTGATGGCATTTCCCACTGCCGTTAAGCGTTGTGGCg                                                                                 >  1:6087008/1‑140 (MQ=255)
                                                       tCAGCTCACGACCATAGTGCAGAGGATTTGCGCTGGCGAGAGAAGGGTGCAGGATCATAAGCGCCTCCGTTAACCTGATACCAGCGCAAACAGTGCATCGTCTGTGCTGGCATTTCCCACTGCCGTTAAGCGTTGTGGCg                                                                                 <  1:6951559/140‑1 (MQ=255)
                                                       tCAGCTCACGACCATAGTGCAGAGGATTTGCGCTGGCGAGAGAAGGGTGCAGGATCATAAGCGCCTCCGTTAACCTGATACCAGCGCAAACAGTGCATCGTCTGTGCTGGCATTTCCCACTGCCGTTAAGCGTTGTGGCg                                                                                 <  2:571396/140‑1 (MQ=255)
                                                       tCAGCTCACGACCATAGTGCAGAGGATTTGCGCTGGCGAGAGAAGGGTGCAGGATCATAAGCGCCTCCGTTAACCTGATACCAGCGCAAACAGTGCATCGTCTGTGCTGGCATTTCCCACTGCCGTTAAGCGTTGTGGCg                                                                                 <  2:5962368/140‑1 (MQ=255)
                                                               cGACCATAGTGCAGAGGATTTGCGCTGGCGAGAGAAGGGTGCAGGATCATAAGCGCCTCCGTTAACCTGATACCAGCGCAAACAGTGCATCGTCTGTGCTGGCATTTCCCACTGCCGTTAAGCGTTGTGGCGAATCAATa                                                                         >  2:4446163/1‑140 (MQ=255)
                                                                gACCATAGTGCAGAGGATTTGCGCTGGCGAGAGAAGGGTGCAGGATCATAAGCGCCTCCGTTAACCTGATACCAGCGCAAACAGTGCATCGTCTGTGCTGGCATTTCCCACTGCCGTTAAGCGTTGTGGCGAATCAATaa                                                                        <  1:1110761/140‑1 (MQ=255)
                                                                     tAGTGCAGAGGATTTGCGCTGGCGAGAGAAGGGTGCAGGATCATAAGCGCCTCCGTTAACCTGATACCAGCGCAAAc                                                                                                                                  >  2:5563131/1‑77 (MQ=255)
                                                                     tAGTGCAGAGGATTTGCGCTGGCGAGAGAAGGGTGCAGGATCATAAGCGCCTCCGTTAACCTGATACCAGCGCAAACAGTGCATCGTCTGTGCTGGCATTTCCCACTGCCGTTAAGCGTTGTGGCGAATCAATAAACTgg                                                                   >  1:2806254/1‑140 (MQ=255)
                                                                      aGTGCAGAGGATTTGCGCTGGCGAGAGAAGGGTGCAGGATCATAAGCGCCTCCGTTAACCTGATACCAGCGCAAACAGTGCATCGTCTGTGCTGGCATTTCCCACTGCCGTTAAGCGTTGTGGCGAATCAATAAACTGGc                                                                  <  1:5395897/140‑1 (MQ=255)
                                                                        tGCAGAGGATTTGCGCTGGCGAGAGAAGGGTGCAGGATCATAAGCGCCTCCGTTAACCTGATACCAGCGCAAACAGTGCATCGTCTGTGCTGGCATTTCCCACTGCCGTTAAGCGTTGTGGCGAATCAATAAACTGGCTg                                                                <  1:159023/140‑1 (MQ=255)
                                                                        tGCAGAGGATTTGCGCTGGCGAGAGAAGGGTGCAGGATCATAAGCGCCTCCGTTAACCTGATACCAGCGCAAACAGTGCATCGTCTGTGCTGGCATTTCCCACTGCCGTTAAGCGTTGTGGCGAATCAATAAACTGGCTg                                                                <  2:1035457/140‑1 (MQ=255)
                                                                          cAGAGGATTTGCGCTGGCGAGAGAAGGGTGCAGGATCATAAGCGCCTCCGTTAACCTGATACCAGCGCAAACAGTGCATCGTCTGTGCTGGCATTTCCCACTGCCGTTAAGCGTTGTGGCGAATCAATAAACTGGCTGAt                                                              <  1:5249496/140‑1 (MQ=255)
                                                                          cAGAGGATTTGCGCTGGCGAGAGAAGGGTGCAGGATCATAAGCGCCTCCGTTAACCTGATACCAGCGCAAACAGTGCATCGTCTGTGCTGGCATTTCCCACTGCCGTTAAGCGTTGTGGCGAATCAATAAACTGGCTGAt                                                              <  2:246976/140‑1 (MQ=255)
                                                                             agGATTTGCGCTGGCGAGAGAAGGGTGCAGGATCATAAGCGCCTCCGTTAACCTGATACCAGCGCAAACAGTGCATCGTCTGTGCTGGCATTTCCCACTGCCGTTAAGCGTTGTGGCGAATCAATAAACTGGCTGATgcg                                                           >  1:2999204/1‑140 (MQ=255)
                                                                             agGATTTGCGCTGGCGAGAGAAGGGTGCAGGATCATAAGCGCCTCCGTTAACCTGATACCAGCGCAAACAGTGCATCGTCTGTGCTGGCATTTCCCACTGCCGTTAAGCGTTGTGGCGAATCAATAAACTGGCTGATgcg                                                           >  2:1809593/1‑140 (MQ=255)
                                                                                  ttGCGCTGGCGAGAGAAGGGTGCAGGATCATAAGCGCCTCCGTTTACCTGATACCAGCGCAAACAGTGCATCGTCTGTGCTGGCATTTCCCACTGCCGTTAAGCGTTGTGGCGAATCAATAAACTGGCTGATGCGCTGaa                                                      <  2:1185514/140‑1 (MQ=255)
                                                                                  ttGCGCTGGCGAGAGAAGGGTGCAGGATCATAAGCGCCTCCGTTAACCTGATACCATCGCAAAAAGTGCATCGTCTGTGCTGGCATTTCCCACTGCCGTTAAGAGTTGTGGAGAATCAATAAACTTGCTGTTGCGCTGaa                                                      <  2:579230/140‑1 (MQ=255)
                                                                                  ttGCGCTGGCGAGAGAAGGGTGCAGGATCATAAGCGCCTCCGTTAACCTGATACCAGCGCAAACAGTGCATCGTCTGTGc                                                                                                                  >  2:4334212/1‑80 (MQ=255)
                                                                                  ttGCGCTGGCGAGAGAAGGGTGCAGGATCATAAGCGCCTCCGTTAACCTGATACCAGCGCAAACAGTGCATCGTCTGTGCTGGCATTTCCCACTGCCGTTAAGCGTTGTGGCGAATCAATAAACTGGCTGATGCGCTGaa                                                      <  1:2092665/140‑1 (MQ=255)
                                                                                  ttGCGCTGGCGAGAGAAGGGTGCAGGATCATAAGCGCCTCCGTTAACCTGATACCAGCGCAAACAGTGCATCGTCTGTGCTGGCATTTCCCACTGCCGTTAAGCGTTGTGGCGAATCAATAAACTGGCTGATGCGCTGaa                                                      <  2:1089713/140‑1 (MQ=255)
                                                                                  ttGCGCTGGCGAGAGAAGGGTGCAGGATCATAAGCGCCTCCGTTAACCTGATACCAGCGCAAACAGTGCATCGTCTGTGCTGGCATTTCCCACTGCCGTTAAGCGTTGTGGCGAATCAATAAACTGGCTGATGCGCTGaa                                                      <  2:4694669/140‑1 (MQ=255)
                                                                                  ttGCGCTGGCGAGAGAAGGGTGCAGGATCATAAGCGCCTCCGTTAACCTGATACCAGCGCAAACAGTGCATCGTCTGTGCTGGCATTTCCCACTGCCGTTAAGCGTTGTGGCGAATCAATAAACTGGCTGATGCGCTGaa                                                      >  2:8276297/1‑140 (MQ=255)
                                                                                  ttGCGCTGGCGAGAGAAGGGTGCAGGATCATAAGCGCCTCCGTTAACCTGATACCAGCGCAAACAGTGCATCGTCTGTGCTGGCATTTCCCACTGCCGTTAAGCGTTGTGGCGAATCAATAAACTGGCTGATGCGCTGaa                                                      >  2:3239764/1‑140 (MQ=255)
                                                                                  ttGCGCTGGCGAGAGAAGGGTGCAGGATCATAAGCGCCTCCGTTAACCTGATACCAGCGCAAACAGTGCATCGTCTGTGCTGGCATTTCCCACTGCCGTTAAGCGTTGTGGCGAATCAATAAACTGGCTGATGCGCTGaa                                                      <  2:2946738/140‑1 (MQ=255)
                                                                                  ttGCGCTGGCGAGAGAAGGGTGCAGGATCATAAGCGCCTCCGTTAACCTGATACCAGCGCAAACAGTGCATCGTATGTGCTGGCATTTCCCACTGCCGTTAAGCGTTGTGGCGAATCAATAAACTGGCTGATGCGCTGaa                                                      <  2:2994181/140‑1 (MQ=255)
                                                                                      gcTGGCGAGAGAAGGGTGCAGGATCATAAGCGCCTCCGTTAACCTGATACCAGCGCAAACAGTGCATCGTCTGTGCTGGCATTTCCCACTGCCGTTAAGCGTTGTGGCGAATCAATAAACTGGCTGATGCGCTGAATGGt                                                  <  2:2319039/140‑1 (MQ=255)
                                                                                      gcTGGCGAGAGAAGGGTGCAGGATCATAAGCGCCTCCGTTAACCTGATACCAGCGCAAACAGTGCATCGTCTGTGCTGGCATTTCCCACTGCCGTTAAGCGTTGTGGCGAATCAATAAACTGGCTGATGCGCTGAATGGt                                                  <  2:4050362/140‑1 (MQ=255)
                                                                                      gcTGGCGAGAGAAGGGTGCAGGATCATAAGCGCCTCCGTTAACCTGATACCAGCGCAAACAGTGCATCGTCTGTGCTGGCATTTCCCACTGCCGTTAAGCGTTGTGGCGAATCAATAAACTGGCTGATGCGCTGAATGGt                                                  <  2:5398456/140‑1 (MQ=255)
                                                                                       cTGGCGAGAGAAGGGTGCAGGATCATAAGCGCCTCCGTTAACCTGATACCAGCGCAAACAGTGCATCGTCTGTGCTGGCATTTCCCACTGCCGTTAAGCGTTGTGGCGAATCAATAAACTGGCTGATGCGCTGAATGGTc                                                 >  2:2202270/1‑140 (MQ=255)
                                                                                         ggCGAGAGAAGGGTGCAGGATCATAAGCGCCTCCGTTAACCTGATACCAGCGCAAACAGTGCATCGTCTGTGCTGGCATTTCCCACTGCCGTTAAGCGTTGTGGCGAATCAATAAACTGGCTGATGCGCTGAATGGTCaa                                               >  1:7782078/1‑140 (MQ=255)
                                                                                         ggCGAGAGAAGGGTGCAGGATCATAAGCGCCTCCGTTAACCTGATACCAGCGCAAACAGTGCATCGTCTGTGCTGGCATTTCCCACTGCCGTTAAGCGTTGTGGCGAAGCAATAAACTGGCTGATGCGCTGAATGGTCaa                                               >  1:7544558/1‑140 (MQ=255)
                                                                                          gCGAGAGAAGGGTGCAGGATCATAAGCGCCTCCGTTAACCTGATACCAGCGCAAACAGTGCATCGTCTGTGCTGGCATTTCCCACTGCCGTTAAGCGTTGTGGCGAATCAATAAACTGGCTGATGCGCTGAATGGTCaaa                                              >  1:2233798/1‑140 (MQ=255)
                                                                                          gCGAGAGAAGGGTGCAGGATCATAAGCGCCTCCGTTAACCTGATACCAGCGCAAACAGTGCATCGTCTGTGCTGGCATTTCCCACTGCCGTTAAGCGTTGTGGCGAATCAATAAACTGGCTGATGCGCTGAATGGTCaaa                                              <  1:6583187/140‑1 (MQ=255)
                                                                                          gCGAGAGAAGGGTGCAGGATCATAAGCGCCTCCGTTAACCTGATACCAGCGCAAACAGTGCATCGTCTGTGCTGGCATTTCCCACTGCCGTTAAGCGTTGTGGCGAATCAATAAACTGGCTGATGCGCTGAATGGTCaaa                                              <  1:3208867/140‑1 (MQ=255)
                                                                                               agaAGGGTGCAGGATCATAAGCGCCTCCGTTAACCTGATACCAGCGCAAACAGTGCATCGTCTGTGCTGGCATTTCCCACTGCCGTTAAGCGTTGTGGCGAATCAATAAACTGGCTGATGCGCTGAATGGTCAAAATAtg                                         >  2:2053127/1‑140 (MQ=255)
                                                                                                  aGGGTGCAGGATCATAAGCGCCTCCGTTAACCTGATACCAGCGCAAACAGTGCATCGTCTGTGCTGGCATTTCCCACTGCCGTTAAGCGTTGTGGCGAATCAATAAACTGGCTGATGCGCTGAATGGTCAAAATATGTGc                                      >  1:3638932/1‑140 (MQ=255)
                                                                                                    ggTGCAGGATCATAAGCGCCTCCGTTAACCTGATACCAGCGCAAACAGTGCATCGTCTGTGCTGGCATTTCCCACTGCCGTTAAGCGTTGTGGCGAATCAATAAACTGGCTGATGCGCTGAATGGTCAAAATATGTGCGt                                    >  1:5077706/1‑140 (MQ=255)
                                                                                                     gTGCAGGATCATAAGCGCCTCCGTTAACCTGATACCAGCGCAAACAGTGCATCGTCTGTGCTGGCATTTCCCACTGCCGTTAAGCGTTGTGGCGAATCAATAAACTGGCTGATGCGCTGAATGGTCAAAATATGTGCGtt                                   >  1:7305550/1‑140 (MQ=255)
                                                                                                        cAGGATCATAAGCGCCTCCGTTAACCTGATACCAGCGCAAACAGTGCATCGTCTGTGCTGGCATTTCCCACTGCCGTTAAGCGTTGTGGCGAATCAATAAACTGGCTGATGCGCTGAATGGTCAAAATATGTGCGTTGGc                                >  2:3380664/1‑140 (MQ=255)
                                                                                                        cAGGATCATAAGCGCCTCCGTTAACCTGATACCAGCGCAAACAGTGCATCGTCTGTGCTGGCATTTCCCACTGCCGTTAAGCGTTGTGGCGAATCAATAAACTGGCTGATGCGCTGAATGGTCAAAATATGTGCGTTGGc                                >  2:6984602/1‑140 (MQ=255)
                                                                                                              cATAAGCGCCTCCGTTAACCTGATACCAGCGCAAACAGTGCATCGTCTGTGCTGGCATTTCCCACTGCCGTTAAGCGTTGTGGCGAATCAATAAACTGGCTGATGCGCTGAATGGTCAAAATATGTGCGTTGGCATCCGt                          <  2:6083836/140‑1 (MQ=255)
                                                                                                                tAAGCGCCTCCGTTAACCTGATACCAGCGCAAACAGTGCATCGTCTGTGCTGGCATTTCCCACTGCCGTTAAGCGTTGTGGCGAATCAATAAACTGGCTGTTGCGCTGACTGGTCAAAATATGTGCGTTGGCATCCGTgg                        >  2:7592683/1‑140 (MQ=255)
                                                                                                                tAAGCGCCTCCGTTAACCTGATACCAGCGCAAACAGTGCATCGTCTGTGCTGGCATTTCCCACTGCCGTTAAGCGTTGTGGCGAATCAATAAACTGGCTGATGCGCTGCATCTGCAAAATATGTGCGATGGCATCCGTgg                        >  1:1838644/1‑140 (MQ=255)
                                                                                                                tAAGCGCCTCCGTTAACCTGATACCAGCGCAAACAGTGCATCGTCTGTGCTGGCATTTCCCACTGCCGTTAAGCGTTGTGGCGAATCAATAAACTGGCTGATGCGCTGAATGGTCAAAATATGTGCGTTGGCATCCGTgg                        >  2:7266/1‑140 (MQ=255)
                                                                                                                acaGCGGCGCCCTTAACCTGTTACCAGCGCAAACAGTGCATCGTCTGTGCTGGCATTTCCCACTGCCGTTAAGCGTTGTGGCGATTCAATAAACTGGCTGATGCGCTGAATGGTCAAAATATGTGCGTTGGCATCCGTgg                        <  2:8886514/138‑1 (MQ=255)
                                                                                                                  aGCGCCTCCGTTAACCTGATACCAGCGCAAACAGTGCATCGTCTGTGCTGGCATTTCCCACTGCCGTTAAGCGTTGTGGCGAATCAATAAACTGGCTGATGCGCTGAATGGTCAAAATATGTGCGTTGGCATCCGTGgcg                      <  1:8737533/140‑1 (MQ=255)
                                                                                                                   gcgcCTCCGTTAACCTGATACCAGCGCAAACAGTGCATCGTCTGTGCTGGCATTTCCCACTGCCGTTAAGCGTTGTGGCGAATCAATAAACTGGCTGATGCGCTGAATGGTCAAAATATGTGCGTTGGCATCCGTGgcgc                     <  1:898440/140‑1 (MQ=255)
                                                                                                                    cgcCTCCGTTAACCTGATACCAGCGCAAACAGTGCATCGTCTGTGCTGGCATTTCCCACTGCCGTTAAGCGTTGTGGCGAATCAATAAACTGGCTGATGCGCTGAATGGTCAAAATATGTGCGTTGGCATCCGTGGCGCt                    <  2:6467287/140‑1 (MQ=255)
                                                                                                                     gcCTCCGTTAACCTGATACCAGCGCAAACAGTGCATCGTCTGTGCTGGCATTTCCCACTGCCGTTAAGCGTTGTGGCGAATCAATAAACTGGCTGATGCGCTGAATGGTCAAAATATGTGCGTTGGCATCCGTGGCGCTg                   >  1:3335665/1‑140 (MQ=255)
                                                                                                                     gcCTCCGTTAACCTGATACCAGCGCAAACAGTGCATCGTCTGTGCTGGCATTTCCCACTGCCGTTAAGCGTTGTGGCGAATCAATAAACTGGCTGATGCGCTGAATGGTCAAAATATGTGCGTTGGCATCCGTGGCGCTg                   >  2:1897999/1‑140 (MQ=255)
                                                                                                                      ccTCCGTTACCCTGCGCCCAGCGCAAACAGTGCAGCGTCTGTGCTGGCATTTCCCCCTGCCGTTAAGCGTTGTGGCGAATCAATAAACTGGCTGATGCGCTGAATGGTCAAACTACGTGCGTGGGCATCCGTGGCGCTGa                  <  1:9361265/140‑1 (MQ=255)
                                                                                                                      ccTCCGTTAACCTGATACCAGCGCAAACAGTGCATCGTCTGTGCTGGCATTTCCCACTGCCGTTAAGCGTTGTGGCGAATCAATAAACTGGCTGATGCGCTGAATGGTCCAAATATGTGCGGTGGCATCCGTGGCGCTGa                  >  2:9422948/1‑140 (MQ=255)
                                                                                                                      ccTCCGTTAACCTGATACCAGCGCAAACAGTGCATCGTCTGTGCTGGCATTTCCCACTGCCGTTAAGCGTTGTGGCGAATCAATAAACTGGCTGATGCGCTGAATGGTCAAAATATGTGCGTTGGCATCCGTGGCGCTGa                  >  1:5615550/1‑140 (MQ=255)
                                                                                                                      ccTCCGTTAACCTGATAACAGCGCAAACAGTGCATCGTCTGTGCTGGCATTTCCCACTGCCGTTAAGCGTTGTGGCGAATCAATAAACTGGCTGATGCGCTGAATGGTCAAAATATGTGCGTTGGCATCCGTGGCGCTGa                  >  2:7801607/1‑140 (MQ=255)
                                                                                                                       cTCCGTTAACCTGATACCAGCGCAAACAGTGCCTCGTCTGTGCTGGCATTTCCCACTGCCGTTAAGAGTGGTGGCGTATCAATAAACTGGCGGAGGCGCGGAATGGTCAAACTCTGTGCGTTGGCATCCGTGGCGGTGAc                 >  1:1011146/1‑140 (MQ=255)
                                                                                                                       cTCCGTTAACCTGATACCAGCGCAAACAGTGCATCGTCTGTGCTGGCATTTCCCACTGCCGTTAAGCGTTGTGGCGAATCAATAAACTGGCTGATGCGCTGAATGGTCAAAATATGTGCGTTGGCATCCGTGGCGCTGAc                 >  1:2540683/1‑140 (MQ=255)
                                                                                                                       cTCCGTTAACCTGATACCAGCGCAAACAGTGCATCGTCTGTGCTGGCATTTCCCACTGCCGTTAAGCGTTGTGGCGAATCAATAAACTGGCTGATGCGCTGAATGGTCAAAATATGTGCGTTGGCATCCGTGGCGCTGAc                 >  2:3128939/1‑140 (MQ=255)
                                                                                                                       cTCCGTTAACCTGATACCAGCGCAAACAGTGCATCGTCTGTGCTGGCATTTCCCACTGCCGTTAAGCGTTGTGGCGAATCAATAAACTGGCTGATGCGCTGAATGGTCAAAATATGTGCGTTGGCATCCGTGGCGCTGAc                 >  1:9130707/1‑140 (MQ=255)
                                                                                                                       cTCCGTTAACCTGATACCAGCGCAAACAGTGCATCGTCTGTGCTGGCATTTCCCACTGCCGTTAAGCGTTGTGGCGAATCAATAAACTGGCTGATGCGCTGAATGGTCAAAATATGTGCGTTGGCATCCGTGGCGCTGAc                 >  1:3778962/1‑140 (MQ=255)
                                                                                                                       atCCGTTAACCTGATACCAGCTCACACAGTGCATCGTCTTTGCTGGCATTTCCCACTTCCTTTAAGAGTTGTGGCGAATCAATATACTGGCTTATGCGCTGAATGGTCAAAATATGTGCTTTGGCATAAGTGGCGCTGag                 >  2:7927056/2‑139 (MQ=255)
                                                                                                                        tCCGTTAACCTGATACCAGCGCAAACAGTGCATCGTCTGTGCTGGCATTTCCCACTGCCGTTAAGCGTTGTGGCGTATCAATAAACTGGCTGATGCGCTGAATGGTCAAAATATGTGCGTTGGCATCCGTGGCGCTGACg                >  2:3573855/1‑140 (MQ=255)
                                                                                                                        tCCGTTAACCTGATACCAGCGCAAACAGTGCATCGTCTGTGCTGGCATTTCCCACTGCCGTTAAGCGTTGTGGCGAATCAATAAACTGGCTGATGCGCTGAATGGTCAAAATATGTGCGTTGGCATCCGTGGCGCTGACg                >  1:8518276/1‑140 (MQ=255)
                                                                                                                        tCCGTTAACCTGATACCAGCGCAAACAGTGCATCGTCTGTGCTGGCATTTCCCACTGCCGTTAAGCGTTGTGGCGAATCAATAAACTGGCTGATGCGCTGAATGGTCAAAATATGTGCGTTGGCATCCGTGGCGCTGACg                <  2:6315678/140‑1 (MQ=255)
                                                                                                                        tCCGTTAACCTGATACCAGCGCAAACAGTGCATCGTCAGTGCTGGCATTTCCCACTGCCGTTAAGCGTTGTGGCGACTCAATAAACTGGCTGATGCGCTGAATGGTCAAACTATGTGCGTTGGCATCCGGGGCGCTGACg                >  2:859017/1‑140 (MQ=255)
                                                                                                                         ccGTTAACCTGATACCAGCGCAAACAGTGCATCGTCTGTGCTGGCATTTCCCACTGCCGTTAAGCGTTGTGGCGAATCAATAAACTGGCTGATGCGCTGAATGGTCAAAATATGTGCGTTGGCATCCGTGGCGCTGACGc               >  1:4049656/1‑140 (MQ=255)
                                                                                                                            ttAACCTGATACCAGCGCAAACAGTGCATCGTCTGTGCTGGCATTTCCCACTGCCGTTAAGCGTTGTGGCGAATCAATAAACTGGCTGATGCGCTGAATGGTCAAAATATGTGCGTTGGCATCCGTGGCGCTGACGCaga            >  2:7149484/1‑140 (MQ=255)
                                                                                                                               aCCTGATCCCCCCGCAAACAGTGCATCGGCTGGGCGGGCAGTTCCCACGGCCGTGAAGCGTTGTGGCGAATCAATAAACTGCCTGAAGCGCTGAATGGTCAAAATATGTGCGTTGGCATCCGTGGCGCTGACGCAGAGaa         <  1:5924254/140‑1 (MQ=255)
                                                                                                                                   gATACCAGCGCAAACAGTGCATCGTCTGTGCTGGCATTTCCCACTGCCGTTAAGCGTTGTGGCGAATCAATAAACTGGCTGATGCGCTGACTGGTCAAAATATGTGCGTTGGCATCCGTGGCGCTGACGCAGAGAagcag     <  1:3487778/140‑1 (MQ=255)
                                                                                                                                    aTACCAGCGCAAACAGTGCATCGTCTGTGCTGGCATTTCCCACTGCCGTTAAGCGTTGTGGCGAATCAATAAACTGGCTGATGCGCTGAATGGTCAAAATATGTGCGTTGGCATCCGTGGCGCTGACGCAGAGAagcagc    >  2:7371140/1‑140 (MQ=255)
                                                                                                                                    aTACCAGCGCAAACAGTGCATCGTCTGTGCTGGCATTTCCCACTGCCGTTAAGCGTTGTGGCGAATCAATAAACTGGCTGATGCGCTGAATGGTCAAAATATGTGCGTTGGCATCCGTGGCGCTGACGCAGAGAagcagc    >  1:9510627/1‑140 (MQ=255)
                                                                                                                                         |                                                                                                                                        
CTGCTGTCCTCAATATCCAGATGCAGGCTCCCAAAATCCAGATTGTCCAATGCCGTCAGCTCACGACCATAGTGCAGAGGATTTGCGCTGGCGAGAGAAGGGTGCAGGATCATAAGCGCCTCCGTTAACCTGATACCGGCGCAAACAGTGCATCGTCTGTGCTGGCATTTCCCACTGCCGTTAAGCGTTGTGGCGAATCAATAAACTGGCTGATGCGCTGAATGGTCAAAATATGTGCGTTGGCATCCGTGGCGCTGACGCAGAGAAGCAGCCA  >  NZ_CP025268/4529340‑4529613

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑