BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP025268	1,356,015	(G)_8→7	intergenic (‑158/+211)	fabI ← / ← ycjD	enoyl‑ACP reductase FabI/DUF559 domain‑containing protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP025268	1,356,008	0	G	.	99.6%	2095.8 / 1.5	450	intergenic (‑151/+218)	fabI/ycjD	enoyl‑ACP reductase FabI/DUF559 domain‑containing protein
Reads supporting (aligned to +/- strand): ref base G (0/1); major base . (147/298); minor base T (2/0); total (150/300)
Fisher's exact test for biased strand distribution p-value = 1.11e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 482 total aligned reads displayed.

TATTGTTGATGCTTGTTGTGCCTGAAAATCAGGCGATTCGTCGTTTTAGTAAACAGTACGAACAGATAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGGCGCTTTTCCCCTCACCC‑TAACC‑CTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCA‑AATATTGTTACCCCAGCAACAAACAGGCTCATACAGCCCCTAACCCTTTCATGGCGATGGCTGGGACGGTT  >  NZ_CP025268/1355870‑1356143

tATTGTTGATGCTTGTTGTGCCTGAAAATCAGGCGATTCGTCGTTTTAGTAAACAGTANGAACAGATAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAgg                                                                                                                                           >  1:6280276/1‑140 (MQ=255)

tATTGTTGATGCTTGTTGTGCCTGAAAATCAGGCGATTCGTCGTTTTAGTAAACAGTACGAACAGATAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAgg                                                                                                                                           >  1:4351296/1‑140 (MQ=255)

tATTGTTGATGCTTGTTGTGCCTGAAAATCAGGCGATTCGTCGTTTTAGTAAACAGTACGAACAGATAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAgg                                                                                                                                           >  2:3020795/1‑140 (MQ=255)

tATTGTTGATGCTTGTTGTGCCTGAAAATCAGGCGATTCGTCGTTTTAGTAAACAGTACGAACAGATAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAgg                                                                                                                                           >  1:1522683/1‑140 (MQ=255)

tATTGTTGATGCTTGTTGTGCCTGAAAATCAGGCGATTCGTCGTTTTAGTAAACAGTACGAACAGATAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAgg                                                                                                                                           >  1:5557440/1‑140 (MQ=255)

  ttgttgATGCTTGTTGTGCCTGAAAATCAGGCGATTCGTCGTTTTAGTAAACAGTACGAACAGATAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAgggg                                                                                                                                         <  1:2176664/140‑1 (MQ=255)

    gttgATGCTTGTTGTGCCTGAAAATCAGGCGATTCGTCGTTTTAGTAAACAGTACGAACAGATAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAgggggg                                                                                                                                       <  1:7758346/140‑1 (MQ=255)

        aTGCTTGTTGTGCCTGAAAATCAGGCGATTCGTCGTTTTAGTAAACAGTACGAACAGATAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAgggggggcgc                                                                                                                                   >  1:4609845/1‑137 (MQ=255)

                tgtgCCTGAAAATCAGGCGATTCGTCGTTTTAGTAAACAGTACGAACAGATAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCA‑GGGGGGGCGCTTTTcccc                                                                                                                          <  1:5516881/140‑1 (MQ=255)

                tgtgCCTGAAAATCAGGCGATTCGTCGTTTTAGTAAACAGTACGAACAGATAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCA‑GGGGGGGCGCTTTTcccc                                                                                                                          <  1:6085966/140‑1 (MQ=255)

                tgtgCCTGAAAATCAGGCGATTCGTCGTTTTAGTAAACAGTACGAACAGATAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCA‑GGGGGGGCGCTTTTcccc                                                                                                                          <  2:1921254/140‑1 (MQ=255)

                 gtgCCTGAAAATCAGGCGATTCGTCGTTTTAGTAAACAGTACGAACAGATAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCA‑GGGGGGGCGCTTTTCCCCt                                                                                                                         <  2:5591174/140‑1 (MQ=255)

                 gtgCCTGAAAATCAGGCGATTCGTCGTTTTAGTAAACAGTACGAACAGATAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCA‑GGGGGGGCGCTTTTCCCCt                                                                                                                         <  1:2726754/140‑1 (MQ=255)

                 gtgCCTGAAAATCAGGCGATTCGTCGTTTTAGTAAACAGTACGAACAGATAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCA‑GGGGGGGCGCTTTTCCCCt                                                                                                                         <  1:4806743/140‑1 (MQ=255)

                 gtgCCTGAAAATCAGGCGATTCGTCGTTTTAGTAAACAGTACGAACAGATAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCA‑GGGGGGGCGCTTTTCCCCt                                                                                                                         <  1:7378201/140‑1 (MQ=255)

                 gtgCCTGAAAATCAGGCGATTCGTCGTTTGAGTAAACAGTACGAACAGATAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCA‑GGGGGGGCGCTTTTCCCCt                                                                                                                         <  2:4745335/140‑1 (MQ=255)

                 gtgCCTGAAAATCAGGCGATTCGTCGTTTGAGTAAACAGTACGAACAGATAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGGTGCCAGGTCCGACCGGAGCAGGCTGCGGCA‑GGGGGGGCGCTTTTCCCCt                                                                                                                         <  2:2641090/140‑1 (MQ=255)

                 gggCCTGAAAATCAGGCGATTCGTCGTTTTAGTAAACAGTACGAACAGATAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGACCGACCGGAGCAGGCTGCGGCA‑GGGGGGGCGCTTTTCCCCt                                                                                                                         <  1:8114178/138‑1 (MQ=255)

                 gagCCAGACAAACACGCGATTCGTCGGTTTAGTAAACAGGACGAACAGCTCCCCGGGTATGCTAATCACCCTGGCGGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCA‑GGGGGGGCGCTTGTCCCCt                                                                                                                         <  2:9242747/138‑1 (MQ=255)

                    ccTGAAAATCAGGCGATTCGTCGTTTTAGTAAACAGTACGAACAGATAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCA‑GGGGGGGCGCTTTTCCCCTCAc                                                                                                                      >  2:3903483/1‑140 (MQ=255)

                    ccTGAAAATCAGGCGATTCGTCGTTTTAGTAAACAGTACGAACAGATAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCA‑GGGGGGGCGCTTTTCCCCTCAc                                                                                                                      >  2:3290500/1‑140 (MQ=255)

                    ccTGAAAATCAGGCGATTCGTCGTTTTAGTAAACAGTACGAACAGATAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCA‑GGGGGGGCGCTTTTCCCCTCAc                                                                                                                      >  2:8987918/1‑140 (MQ=255)

                       gAAAATCAGGCGATTCGTCGTTTTAGTAAACAGTACGAACAGATAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCA‑GGGGGGGCGCGTTTCCCCTCAccca                                                                                                                   >  1:7461829/1‑139 (MQ=255)

                        aaaaTCAGGCGATTCGTCGTTTTAGTAAACAGTACGAACAGATAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCA‑GGGGGGGCGCTTTTCCCTTCACCC‑Ta                                                                                                                 <  1:9307383/140‑1 (MQ=255)

                        aaaaTCAGGCGATTCGTCGTTTTAGTAAACAGTACGAACAGATAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCA‑GGGGGGGCGCTTTTCCCCTCAcccga                                                                                                                  >  2:2410077/1‑138 (MQ=255)

                        aaaaTCAGGCGATTCGTCGTTTTAGTAAACAGTACGAACAGATAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCA‑GGGGGGGCGCTTTTCCCCTCACCC‑Ta                                                                                                                 <  1:5961149/140‑1 (MQ=255)

                        aaaaTCAGGCGATTCGTCGTTTTAGTAAACAGTACGAACAGATAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCA‑GGGGGGGCGCGTTTCCCCTCACCC‑Ta                                                                                                                 >  2:3648971/1‑140 (MQ=255)

                           aTCAGGCGATTCGTCGTTTTAGTAAACAGTACGAACAGATAAACGGTTATTATAATCCACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCA‑GGGGGGGCGCTTTTCCCCTCACCC‑TAAcc                                                                                                              <  2:5776971/140‑1 (MQ=255)

                             cAGGCGATTCGTCGTTTTAGTAAACAGTACGAACAGATAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCA‑GGGGGGGCGCTTTTCCCCTCACCC‑TAACC‑ct                                                                                                           <  1:6633098/140‑1 (MQ=255)

                              cggCGATTCGTCGTTATAGTAAACAGTACGAACAGATACACGGGTAGTAGACTCAACCGGGCTGTGAGTAGCGATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCA‑GGGGGGGCGC‑TTTCCCCTCACCC‑TAACC‑ctct                                                                                                         <  2:2286881/139‑1 (MQ=255)

                               ggCGATTCGTCGTTTTAGTAAACAGTACGAACAGATAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCA‑GGGGGGGCGCTTTTCCCCTCACCC‑TAACC‑ctct                                                                                                         <  1:5335370/140‑1 (MQ=255)

                               ggCGATTCGTCGTTTTAGTAAACAGTACGAACAGATAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCA‑GGGGGGGCGCTTTTCCCCTCACCC‑TAACC‑ctct                                                                                                         >  2:775307/1‑140 (MQ=255)

                               ggCGATTCGTCGTTTTAGTAAACAGTACGAACAGATAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCA‑GGGGGGGCGCTTTTCCCCTCACCC‑TAACC‑ctct                                                                                                         <  1:1458481/140‑1 (MQ=255)

                               ggCGATTCGTCGTTTTAGTAAACAGTACGAACAGATAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCA‑GGGGGGGCGCTTTTCCCCTCACCC‑TAACC‑ctct                                                                                                         <  2:4459033/140‑1 (MQ=255)

                               ggCGATTCGTCGTTTTAGTAAACAGTACGAACAGATAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCA‑GGGGGGGCGCTTTTCCCCTCACCC‑TAACC‑ctct                                                                                                         <  1:3593079/140‑1 (MQ=255)

                                    ttCGTCGTTTTAGTAAACAGTACGAACAGATAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCA‑GGGGGGGCGCTTTTCCCCTCACCC‑TAACC‑CTCTCCCCa                                                                                                    >  2:7149145/1‑140 (MQ=255)

                                     tcgtcgTTTTAGTAAACAGTACGAACAGATAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGACAGGTCCGACCGGAGCAGGCTGCGGCA‑GGGGGGGCGCTTTTCCCCTCACCC‑AAACC‑CACTCCCCag                                                                                                   >  2:5291548/1‑140 (MQ=255)

                                      cgtcgtTTTAGTAAACAGTACGAACAGATAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCA‑GGGGGGGCGCTTTTCCCCTCACCC‑TAACC‑CTCTCCCCaga                                                                                                  <  2:4028946/140‑1 (MQ=255)

                                           ttttAGTAAACAGTACGAACAGATAAACGTTTATTATAATCAAACTGGCTGTGAGTATCTATAGTTGCCAGGTCCGACAGGAGCAGGCTGCTGCA‑GGGGGGGCGCTTTTCACCTCACCC‑TAACC‑CTCTCCCCAGAGGGGc                                                                                             <  2:129378/140‑1 (MQ=255)

                                             ttAGTAAACAGTACGAACAGATAACCGGTTATGATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCA‑GGGGGGGCGCTTTTCCCCTCACCC‑TACCC‑CTCGCCCCAGAGGGGCGa                                                                                           <  1:7307667/140‑1 (MQ=255)

                                             ttAGTAAACAGTACGAACAGATAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCA‑GGGGGGGCGCTTTTCCCCTCACCC‑TAACC‑CTCTCCCCAGAGGGGCGa                                                                                           <  1:8979196/140‑1 (MQ=255)

                                             ttAGTAAACAGTACGAACAGATAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCA‑GGGGGGGCGCTTTTCCCCTCACCC‑TAACC‑CTCTCCCCAGAGGGGCGa                                                                                           <  1:8304642/140‑1 (MQ=255)

                                             ttAGTAAACAGTACGAACAGATAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCA‑GGGGGGGCGCTTTTCCCCTCACCC‑TAACC‑CTCTCCCCAGAGGGGCGa                                                                                           <  1:7579018/140‑1 (MQ=255)

                                             ctAGTAAACAGTACGCACAGATAACCGGATATTAGAATCACCCTGGCAGAGAGTAGCTATAGTTGCCACGTCCGACCGGAGCAGGCTGCGGCA‑GGGGGGGCGCTTTTCCCCACACCC‑TAACC‑CTCTCCCCAGAGTGGCGa                                                                                           <  2:1378187/139‑1 (MQ=255)

                                                  aaaCAGTACGAACAGATAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCA‑GGGGGGGCGCTTTTCCCCTCACCC‑TAACC‑CTCTCCCCAGAGGGGCGAGGGGa                                                                                      <  2:6853267/140‑1 (MQ=255)

                                                  aaaCAGTACGAACAGATAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCA‑GGGGGGGCGCTTTTCCCCTCACCC‑TAACC‑CTCTCCCCAGAGGGGCGAGGGGa                                                                                      <  1:8014407/140‑1 (MQ=255)

                                                  aaaCAGTACGAACAGATAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCA‑GGGGGGGCGCTTTTCCCCTCACCC‑TAACC‑CTCTCCCCAGAGGGGCGAGGGGa                                                                                      <  1:1124441/140‑1 (MQ=255)

                                                  aaaCAGTACGAACAGATAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGGTGCCAGGTCCGACCGGAGCAGGCTGCGGCA‑GGGGGGGCGCTTTTCCCCTCACCC‑TAACC‑CTCTCCCCAGAGGGGCGAGGGGa                                                                                      <  1:1132473/140‑1 (MQ=255)

                                                        tccGAACAGATCAACGGTTATGATAATCACACGGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCA‑GGGGGGGCGCTTTTCCCCTCACCC‑TAACC‑CTCTCCCCAGAGGGGCGAGGGGACCGTAt                                                                                <  2:3436276/138‑1 (MQ=37)

                                                        tACGAACAGATAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCA‑GGGGGGGCGCTTTTCCCCTCACCC‑TAACC‑CTCTCCCCAGAGGGGCGAGGGGACCGTAt                                                                                <  1:8543818/140‑1 (MQ=255)

                                                        tACGAACAGATAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCA‑GGGGGGGCGCTTTTCCCCTCACCC‑TAACC‑CTCTCCCCAGAGGGGCGAGGGGACCGTAt                                                                                >  2:6100241/1‑140 (MQ=255)

                                                        tACGAACAGATAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCA‑GGGGGGGCGCTTTTCCCCTCACCC‑TAACC‑CTCTCCCCAGAGGGGCGAGGGGACCGTAt                                                                                >  2:7826242/1‑140 (MQ=255)

                                                        tACGAACAGATAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCA‑GGGGGGGCGCTTTTACCCCCACCC‑AAACC‑CACTCCCCAGACGGGCGAggggcacggat                                                                                >  1:4413158/1‑133 (MQ=255)

                                                        tACGAACAGATAAACGGTTATTATAATCAACATGGCTGTGAGTAGCTATAGTTGCCATGTCCGACCGGAGAAGGCTGCGGCA‑GGGGGGGCGCTTTTCCCCACCCCC‑TAACC‑CTCTCCCCAGAGGGGCGAGGGGACCggag                                                                                >  2:8413018/1‑137 (MQ=38)

                                                               aGATAAACGGTTATTATACTCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCA‑GGGGGGGCGCATTTCCCCTCACCC‑TAACC‑CTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCa‑a                                                                        <  2:6701293/140‑1 (MQ=255)

                                                               aGATAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCA‑GGGGGGGCGCTTTTCCCCTCACCC‑TAACC‑CTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCa‑a                                                                        <  1:4176108/140‑1 (MQ=255)

                                                                gATACACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCA‑GGGGGGGCGCTTTTCCCCTCACCC‑TAACC‑CTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCa‑aa                                                                       <  1:3898420/140‑1 (MQ=255)

                                                                gATAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCA‑GGGGGGGCGCTTTTCCCCTCACCC‑TAACC‑CTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCa‑aa                                                                       >  2:6426529/1‑140 (MQ=255)

                                                                gATAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCA‑GGGGGGGCGCTTTTACCCACACCC‑AAACC‑CACGCCCCCCTGGGGCGAGGGGGCCGGAGTGTGCa‑aa                                                                       >  1:169006/1‑140 (MQ=255)

                                                                gATAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCA‑GGGGGGGCGCGTTTCCCCTCACCC‑GAACC‑CGCTCCCCCGAGGGGCGAGGGGACCGTATTGGGCa‑aa                                                                       >  1:6741713/1‑140 (MQ=255)

                                                                gATAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCA‑GGGGGGGCGCGTTTCCCCGCACCC‑TAACC‑CACTCCCCAGAGGGGCGAGGGGACCGGATtgctgtct                                                                        >  2:8214767/1‑134 (MQ=38)

                                                                 aTAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCA‑GGGGGGGCGCTTTTCCCCTCACCC‑TAACC‑CTCTCCCCAGAGGGGCGATGGGACCGTATTGTGCA‑Aat                                                                      <  2:5965461/140‑1 (MQ=255)

                                                                 aTAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCA‑GGGGGGGCGCTTTTCCCCTCACCC‑TAACC‑CTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCA‑Aat                                                                      <  2:7404145/140‑1 (MQ=255)

                                                                    aaCGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCA‑GGGGGGGCGCTTTTCCCCTCACCC‑TAACC‑CTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCA‑AATAtt                                                                   <  1:7895992/140‑1 (MQ=255)

                                                                      cGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCA‑GGGGGGGCGCTTTTCCCCACAACC‑TAAAC‑CCAACCCCACAGGGGGGCGGGGACCCTATTTTGGC‑AAAATTGt                                                                 >  1:3445944/1‑140 (MQ=255)

                                                                            ttatAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCA‑GGGGGGGCGCTTTTCCCCTCACCC‑TAACC‑CTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCA‑AATATTGTTAcccc                                                           <  1:4954354/140‑1 (MQ=255)

                                                                               taATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCA‑GGGGGGGCGCTTTTCCCCTCACCC‑TAACC‑CTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCA‑AATATTGTTACCCCAGc                                                        <  1:2610499/140‑1 (MQ=255)

                                                                                aaTCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCA‑GGGGGGGCGCTTTTCCCCGCACCC‑AAACC‑CGCGCCCCAGAGGGGCGAGGGGGCCCGATTGTGCA‑AAAATTGTTAACCCCGca                                                       >  1:1360135/1‑140 (MQ=255)

                                                                                 aTCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCA‑GGGGGGGCGCTTTTACCCGCACCC‑AAAAC‑CACACCCCCCAGGGGCGCGGGGACCCTATTTTGCA‑AAAATTTTTAACCCaccca                                                      >  1:308445/1‑136 (MQ=255)

                                                                                     cccTGGCTGTGAGTAGCTATAGGTGCCAGGTCCGACCGGAGCAGGCTGCGGCA‑GGGGGGGCGCTTTTCCCCTCACCC‑TAACC‑CTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCA‑CATATTGTTACCCCAGCaacaaa                                                  <  1:8711412/139‑1 (MQ=255)

                                                                                     cccTGGCTGTGAGTAGCTAGAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGC‑CGGGGGGGCGCTTTTCCCCTCACCC‑TAACC‑CACTCCCCAGCGGGGCGAGGGGACCGTATGGTGCA‑AATATTGTTACCCCAGCaacaaa                                                  <  2:352331/139‑1 (MQ=38)

                                                                                     aCCTGTCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCA‑GGGGGGGCGCTTGTCCCCTCACCC‑TAACC‑CTCTACCCAGAGGGGCGAGGGGACCGTATTGTGCA‑AATATTGTTACCCCCGCaacaaa                                                  <  2:3199252/140‑1 (MQ=38)

                                                                                     aCCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCA‑GGGGGGGCGCTTTTCCCCTCACCC‑TAACC‑CTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCA‑AATATTGTTACCCCAGCaacaaa                                                  <  1:6488007/140‑1 (MQ=255)

                                                                                      ccTGGCTGTGAGTAGCTATAGTTGCCCGGTCCGACCGGAGCAGGCTGCGGCA‑GGGGGGGCGCTTTTCCCCTCACCC‑AAACC‑CACTCCCCAGAGGGGCGAGGGGACCGTATTGTGCA‑AAATTTGTTACCCCAGCaacacac                                                 >  2:9128534/1‑140 (MQ=38)

                                                                                      ccTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCA‑GGGGGGGCGCTTTTCCCCGCACCC‑TAACC‑CAAACCCCCGATGGGCGAGGGGACCCTATTTTTCA‑AATATTGTTAACCCAGCaacaaaa                                                 >  2:2075934/1‑139 (MQ=255)

                                                                                              tgAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCA‑GGGGGGGCGCTTTTCCCCTCACCC‑TAACC‑CTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCA‑AATATTGTTAACCCAGCAACAAACAGGCTCAt                                         >  2:9341727/1‑140 (MQ=255)

                                                                                               gAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCA‑GGGGGGGCGCTTTTCCCCTCACCC‑TAACC‑CTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCA‑AATATTGTTACCCCAGCAACAAACAGGCTCATa                                        <  1:2519312/140‑1 (MQ=255)

                                                                                                aGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCA‑GGGGGGGCGCTTTTCCCCTCACCC‑TAACC‑CTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCA‑AATATTGTTACCCCAGCAACAAACAGGCTCATAc                                       >  2:5030408/1‑140 (MQ=255)

                                                                                                aGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCA‑GGGGGGGCGCTTTTCCCCTCACCC‑TAACC‑CTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCA‑AATATTGTTACCCCAGCAACAAACAGGCTCATAc                                       <  1:9206926/140‑1 (MQ=255)

                                                                                                aGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCA‑GGGGGGGCGCTTTTCCCCTCACCC‑TAACC‑CTCTCCCCAGAGGGGCGAGGGGACCGTATTGGGCA‑AATATTGTTACCCCAGCAACAAACAGGCTCATAc                                       >  2:3720010/1‑140 (MQ=255)

                                                                                                       ataGTTGCCAGGTCCGCCCGGAGCAGGCTGCGGCA‑GGGGGGGCGCTTTTCCCCTCACCC‑TAACC‑CTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCA‑AATATTGTTACCCCAGCAACAAACAGGCTCATACAGCCCCt                                <  1:6916432/140‑1 (MQ=255)

                                                                                                        taGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCA‑GGGGGGGCGCTTTTCCCCTCACCC‑GAACC‑CACACCCCAGAGGGGCGAGGGGACCCGATTGTGCA‑AAAAATGTTAACCCAGCAACAAACAGGCGCATACAGccccaa                               >  2:1142659/1‑138 (MQ=37)

                                                                                                          gTTGCCAGGTCCGACCGGAGCAGGCTGCGGCA‑GGGGGGGCGCTTTTCCCCCCACCC‑CAAAC‑CCCACCCCCCCGGGGGGGGGGGGCCCGATTGTGCACAAAATTTTTA‑ACCACCCACAAAAACGCGCAAAAAGCCCCAAAc                             >  1:7173516/1‑140 (MQ=255)

                                                                                                            tGCCAGGTCCGACCGGAGCAGGCTGCGGCA‑GGGGGGGCGCTTTTCCCCt                                                                                                                         >  1:2940947/1‑49 (MQ=255)

                                                                                                            tGCCAGGTCCGACCGGAGCAGGCTGCGGCA‑GGGGGGGCGCTTTTCCCCt                                                                                                                         <  2:2940947/49‑1 (MQ=255)

                                                                                                            tGCCAGGTCCGACCGGAGCAGGCTGCGGCA‑GGGGGGGCGCTTTTCCCCGCACCC‑AAAAC‑CACTCCCCAGAGGGGCGAGGGGACCGGATTGTGCA‑AATATTTTTAACCCCGCAACAAAAAGGCTCATACAGCCCCAAAccc                           >  1:7050355/1‑140 (MQ=37)

                                                                                                              ccAGGTCCGACCGGAGCAGGCTGCGGCA‑GGGGGGGCGCTTTTCCCCACACCC‑TAACC‑CACTCCCCAGATGGGCGAGGGGACCGTATTGTGCA‑AATATTGTTACCCCAGCAACAAAAAGGCTCAAAAATCCCCTAAACCtt                         >  1:4118554/1‑140 (MQ=255)

                                                                                                               cAGGTCCGACCGGAGCAGGCTGCGGCA‑GGGGGGGCGCTTTTCCCCTCACCC‑TAACC‑CTCTACCCAGAGGGGCGAGGGGACCGTATTGTGCA‑AATATTGTTTCCCCAGCAACAAAAAGTCGCATACATCCCCTAACCCttt                        >  2:7210594/1‑140 (MQ=37)

                                                                                                                  gTCCGACCGGAGCAGGCTGCGGCA‑GGGGGGGCGCTTTTGCCCTCCCCCTTAACCACGACCCCCAGGGGGGCG‑GGGGACCG‑ATTGTGGC‑AATATTTTTTCCCCCCCAAAAAACACGGAGGTTCAGCCCCGAAccccgtctt                     >  1:6370238/1‑134 (MQ=255)

                                                                                                                      gACCGGAGCAGGCTGCGGCA‑GGGGGGGCGCTTTTCCCCTCACCC‑TAACC‑CTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCA‑AATATTGTTACCCCAGCAACAAACAGGCTCATACAGCCCCTAACCCTTTCATGGCg                 <  1:2773761/140‑1 (MQ=255)

                                                                                                                      gACCGGAGCAGGCTGCGGCA‑GGGGGGGCGCTTTTCCCCTCACCC‑TAACC‑CTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCA‑AATATTGTTACCCCAGCAACAAACAGGCTCATACAGCCCCTAACCCTTTCATGGCg                 <  1:9438766/140‑1 (MQ=255)

                                                                                                                      gACCGGAGCAGGCTGCGGCA‑GGGGGGGCGCTTTTCCCCTCACCC‑TAACC‑CTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCA‑AATATTGTTACCCCAGCAACAAACAGGCTCATACAGCCCCTAACCCTTTCATGGCg                 <  2:1709465/140‑1 (MQ=255)

                                                                                                                      gACCGGAGCAGGCTGCGGCA‑GGGGGGGCGCTTTTCCCCTCACCC‑TAACC‑CTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCA‑AATATTGTTACCCCAGCAACAAACAGGCTCATACAGCCCCTAACCCTTTCATGGCg                 <  1:2354544/140‑1 (MQ=255)

                                                                                                                            cgCAGGGTGCGGCAGGGGGGGGCGCTTTTCCCCTCACCC‑TAACC‑CTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCA‑AATATTGTTACCCCAGCAACAAACAGGCTCATACAGCCCCTAACCCTTTCATGGCGATGGc            <  1:1211366/139‑1 (MQ=255)

                                                                                                                            aGCAGGCTGCGGCA‑GGGGGGGCGCTTTTCCCCTCACCC‑TAACC‑CTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCA‑CATATTGTTACCCCAGCAACAAACAGGCTCATACAGCCCCTAACCCTTTCATGGCGATGGCt           >  2:7971858/1‑140 (MQ=255)

                                                                                                                                ggCTGCGGCA‑GGGGGGGCGCTTTTCCCCTCACCC‑TAACC‑CTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCA‑AATATTGTTACCCCAGCAACAAACAGGCTCATACAGCCCCTAACCCTTTCATGGCGATGGCTGGGa       <  2:1538847/140‑1 (MQ=255)

                                                                                                                                ggCTGCGGCA‑GGGGGGGCGCTTTTCCCCTCACCC‑TAACC‑CTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCA‑AATATTGTTACCCCAGCAACAAACAGGCTCATACAGCCCCTAACCCTTTCATGGCGATGGCTGGGa       <  2:7319009/140‑1 (MQ=255)

                                                                                                                                ggCTGCGGCA‑GGGGGGGCGCTTTTCCCCTCACCC‑TAACC‑CTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCA‑AATATTGTTACCCCAGCAACAAACAGGCTCATACAGCCCCTAACCCTTTCATGGCGATGGCTGGGa       <  2:8714650/140‑1 (MQ=255)

                                                                                                                                ggCTGCGGCA‑GGGGGGGCGCTTTTCCCCTCACCC‑TAACC‑CTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCA‑AATATTGTTACCCCAGCAACAAACAGGCTCATACAGCCCCTAACCCTTTCATGGCGATGGCTGGGa       <  2:422345/140‑1 (MQ=255)

                                                                                                                                     cGGCA‑GGGGGGGCGCTTTTCCCCTCACCC‑TAACC‑CTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCa‑a                                                                        <  1:1610733/70‑1 (MQ=255)

TATTGTTGATGCTTGTTGTGCCTGAAAATCAGGCGATTCGTCGTTTTAGTAAACAGTACGAACAGATAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGGCGCTTTTCCCCTCACCC‑TAACC‑CTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCA‑AATATTGTTACCCCAGCAACAAACAGGCTCATACAGCCCCTAACCCTTTCATGGCGATGGCTGGGACGGTT  >  NZ_CP025268/1355870‑1356143

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`