Predicted mutation
evidence seq id position mutation annotation gene description
RA NZ_CP025268 4,331,820 (G)7→6 pseudogene (473/817 nt) rdcB → YjcZ‑like family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0252684,331,8140G.99.2% 2846.4 / 10.7 605pseudogene (467/817 nt)rdcBYjcZ‑like family protein
Reads supporting (aligned to +/- strand):  ref base G (1/4);  new base . (245/353);  total (246/359)
Fisher's exact test for biased strand distribution p-value = 6.53e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 630 total aligned reads displayed.

GAAAAGGCGTTAACGCAACGAGGCCTACTGGTGCAGGCAGGTGATCAGAGCGAGCAAATTTTTACCCGCTGGCGTGCTGGGGCGTATAACGCCTGGTCGTTGCCTGGGCGCTGTTTTATCGTTCTGGAGGAGTTGCGCTGGGGGGGCATTTGGCGATGCCTGCCGTCTGGGAAGCCCGCAAGCGGTGGCGTTGTTGCTGGGTGATTTGCTCGAGAAAGCGACACAACATCTGGCAGAGAGTATCAATGCGGCACCGACCACGCGTCACTATTACC  >  NZ_CP025268/4331675‑4331949
                                                                                                                                           |                                                                                                                                       
   aaGGCGTTAACGCAACGAGGCCTACTGGTGCAGGCAGGTGATCAGAGCGAGCAAATTTTTACCCGCTGGCGTGCTGGGGCGTATAACGCCTGGTCGTTGCCTGGGCGCTGTTTTATCGTTCTGGAGGAGTTGCGCTgggg                                                                                                                                      <  1:7084369/140‑1 (MQ=255)
    aGGCGTTAACGCAACGAGGCCTACTGGTGCAGGCAGGTGATCAGAGCGAGCAAATTTTTACCCGCTGGCGTGCTGGGGCGTATAACGCCTGGTCGTTGCCTGGGCGCTGTTTTATCGTTCTGGAGGAGTTGCGCTggggg                                                                                                                                     >  2:3330583/1‑140 (MQ=255)
        gTTAACGCAACGAGGCCTACTGGTGCAGGCAGGTGATCAGAGCGAGCAAATTTTTACCCGCTGGCGTGCTGGGGCGTATAACGCCTGGTCGTTGCCTGGGCGCTGTTTTATCGTTCTGGAGGAGTTGCGCTggggggcat                                                                                                                                 <  1:7363094/140‑4 (MQ=255)
                   gAGGCCTACTGGTGCAGGCAGGTGATCAGAGCGAGCAAATTTTTACCCGCTGGCGTGCTGGGGCGTATAACGCCTGGTCGTTGCCTGGGCGCTGTTTTATCGTTCTGGAGGAGTTGCGCT‑GGGGGGCATTTGGCGAtgcc                                                                                                                     <  2:2666362/140‑1 (MQ=255)
                    aGGCCTACTGGTGCAGGCAGGTGATCAGAGCGAGCAAATTTTTACCCGCTGGCGTGCTGGGGCGTATAACGCCTGGTCGTTGCCTGGGCGCTGTTTTATCGTTCTGGAGGAGTTGCGCT‑GGGGGGCATTTGGCGAtgcct                                                                                                                    <  2:8474582/140‑1 (MQ=255)
                    aGGCCTACTGGTGCAGGCAGGTGATCAGAGCGAGCAAATTTTTACCCGCTGGCGTGCTGGGGCGTATAACGCCTGGTCGTTGCCTGGGCGCTGTTTTATCGTTCTGGAGGAGTTGCGCT‑GGGGGGCATTTGGCGAtgcct                                                                                                                    <  1:3650269/140‑1 (MQ=255)
                    aGGCCTACTGGTGCAGGCAGGTGATCAGAGCGAGCAAATTTTTACCCGCTGGCGTGCTGGGGCGTATAACGCCTGGTCGTTGCCTGGGCGCTGTTTTATCGTTCTGGAGGAGTTGCGCT‑GGGGGGCATTTGGCGAtgcct                                                                                                                    <  2:6754854/140‑1 (MQ=255)
                    aGGCCTACTGGTGCAGGCAGGTGATCAGAGCGAGCAAATTTTTACCCGCTGGCGTGCTGGGGCGTATAACGCCTGGTCGTTGCCTGGGCGCTGTTTTATCGTTCTGGAGGAGTTGCGCT‑GGGGGGCATTTGGCGAtgcct                                                                                                                    <  1:6041640/140‑1 (MQ=255)
                    aGGCCTACTGGTGCAGGCAGGTGATCAGAGCGAGCAAATTTTTACCCGCTGGCGTGCTGGGGCGTATAACGCCTGGTCGTTGCCTGGGCGCTGTTGTATCGTTCTGGGGGAGTTGCGCT‑GGGGGGCATTTGGCGAtgcct                                                                                                                    >  2:3987461/1‑140 (MQ=255)
                      gCCTACTGGTGCAGGCAGGTGATCAGAGCGAGCAAATTTTTACCCGCTGGCGTGCTGGGGCGTATAACGCCTGGTCGTTGCCTGGGCGCTGTTTTATCGTTCTGGAGGAGTTGCGCT‑GGGGGGCATTTGGCGAtgcctgc                                                                                                                  >  1:9245585/1‑140 (MQ=255)
                      gCCTACTGGTGCAGGCAGGTGATCAGAGCGAGCAAATTTTTACCCGCTGGCGTGCTGGGGCGTATAACGCCTGGTCGTTGCCTGGGCGCTGTTTTATCGTTCTGGAGGAGTTGCGCT‑GGGGGGCATTTGGCGAtgcctgc                                                                                                                  <  2:1440905/140‑1 (MQ=255)
                        cttcgggTGCAGGCAGGTGATCAGAGCGAGCAAATTTTTACCCGCTGGCGTGCTGGGGCGTATAACGCCTGGTCGTTGCCTGGGCGCTGTTTTATCGTTCTGGAGGAGTTGCGCT‑GGGGGGCATTTGGCGATGCCTGCCg                                                                                                                <  2:5160145/135‑1 (MQ=255)
                        cTACTGGTGCAGGCAGGTGATCAGAGCGAGCAAATTTTTACCCGCTGGCGTGCTGGGGCGTATAACGCCTGGTCGTTGCCTGGGCGCTGTTTTATCGTTCTGGAGGAGTTGCGCT‑GGGGGGCATTTGGCGATGCCTGCCg                                                                                                                <  1:1863094/140‑1 (MQ=255)
                          aCTGGTGCAGGCAGGTGATCAGAGCGAGCAAATTTTTACCCGCTGGCGTGCTGGGGCGTATAACGCCTGGTCGTTGCCTGGGCGCTGTTTTATCGTTCTGGAGGAGTTGCGCT‑GGGGGGCATTTGGCGATGCCTGCCGTc                                                                                                              >  1:976176/1‑140 (MQ=255)
                          aCTGGTGCAGGCAGGTGATCAGAGCGAGCAAATTTTTACCCGCTGGCGTGCTGGGGCGTATAACGCCTGGTCGTTGCCTGGGCGCTGTTTTATCGTTCTGGAGGAGTTGCGCT‑GGGGGGCATTTGGCGATGCCTGCCGTc                                                                                                              >  2:4632417/1‑140 (MQ=255)
                          aCTGGTGCAGGCAGGTGATCAGAGCGAGCAAATTTTTACCCGCTGGCGTGCTGGGGCGTATAACGCCTGGTCGTTGCCTGGGCGCTGTTTTATCGTTCTGGAGGAGTTGCGCT‑GGGGGGCATTTGGCGATGCCTGCCGTc                                                                                                              >  1:1660566/1‑140 (MQ=255)
                             ggTGCAGGCAGGTGATCAGAGCGAGCAAATTTTTACCCGCTGGCGTGCTGGGGCGTATAACGCCTGGTCGTTGCCTGGGCGCTGTTTTATCGTTCTGGAGGAGTTGCGCT‑GGGGGGCATTTGGCGATGCCTGCCGTCTgg                                                                                                           >  1:6350181/1‑140 (MQ=255)
                              gTGCAGGCAGGTGATCAGAGCGAGCAAATTATTACCCGCTGGCGTGCTGGGGCGTATAACGCCTGGTCGTTGCCTGGGCGCTGTTTTATCGTTCTGGAGGAGTTGCGCT‑GGGGGGCATTTGGCGATGCCTGCCGTCTggg                                                                                                          <  1:4900244/140‑1 (MQ=255)
                               tGCAGGCAGGTGATCAGAGCGAGCAAATTTTTACCCGCTGGCGTGCTGGGGCGTATAACGCCTGGTCGTTGCCTGGGCGCTGTTTTATCGTTCTGGAGGAGTTGCGCT‑GGGGGGCATTTGGCGATGCCTGCCGTCTGGGa                                                                                                         >  2:7052699/1‑140 (MQ=255)
                                 caggcaggTGATCCGAGCGAGCAAATATTTACCCGCTGGCGTGCTGGGGCGTATAACGCCTGGTCGTTGCCGGGGCGCTGTGTGATCGTTCTGGAGGAGTTGAGCT‑GGTGGGCCTTTGGCGATGCCTGCCGTCTGGGAAg                                                                                                       >  2:6317944/1‑140 (MQ=255)
                                 caggcaggTGATCAGAGCGAGCAAATTTTTACCCGCTGGCGTGCTGGGGCGTATAACGCCTGGTCGTTGCCTGGGCGCTGTTTTATCGTTCTGGAGGAGTTGCGCT‑GGGGGGCATTTGGCGATGCCTGCCGTCTGGGAAg                                                                                                       >  2:16685/1‑140 (MQ=255)
                                 caggcaggTGATCAGAGCGAGCAAATTTTTACCCGCTGGCGTGCTGGGGCGTATAACGCCTGGTCGTTGCCTGGGCGCTGTTTTATCGTTCTGGAGGAGTTGCGCT‑GGGGGGCATTTGGCGATGCCTGCCGTCTGGGAAg                                                                                                       >  1:5897190/1‑140 (MQ=255)
                                 caggcaggTGATCAGAGCGAGCAAATTTTTACCCGCTGGCGTGCTGGGGCGTATAACGCCTGGTCGTTGCCTGGGCGCTGTTTTATCGTTCTGGAGGAGTTGCGCT‑GGGGGGCATTTGGCGATGCCTGCCGTCTGGGAAg                                                                                                       >  2:4295003/1‑140 (MQ=255)
                                 caggcaggTGATCAGAGCGAGCAAATTTTTACCCGCTGGCGTGCTGGGGCGTATAACGCCTGGTCGTTGCCTGGGCGCTGTGTTATCGTTCTGGAGGAGTTGCGCT‑GGGGGGCATTTGGCGATGCCTGCCGTCTGGGAAg                                                                                                       >  1:426150/1‑140 (MQ=255)
                                   ggcaggTGATCAGAGCGAGCAAATTTTTACCCGCTGGCGTGCTGGGGCGTATAACGCCTGGTCGTTGCCTGGGCGCTGTTTTATCGTTCTGGAGGAGTTGCGCT‑GGGGGGCATTTGGCGATGCCTGCCGTCTGGGAAGcc                                                                                                     >  1:338795/1‑140 (MQ=255)
                                    gcaggTGATCAGAGCGAGCAAATTTTTACCCGCTGGCGTGCTGGGGCGTATAACGCCTGGTCGTTGCCTGGGCGCTGTTTTATCGTTCTGGAGGAGTTGCGCT‑GGGGGGCATTTGGCGATGCCTGCCGTCTGGGAAGccc                                                                                                    >  1:5795926/1‑140 (MQ=255)
                                    gcaggTGATCAGAGCGAGCAAATTTTTACCCGCTGGCGTGCTGGGGCGTATAACGCCTGGTCGTTGCCTGGGCGCTGTTTTATCGTTCTGGAGGAGTTGCGCT‑GGGGGGCATTTGGCGATGCCTGCCGTCTGGGAAGccc                                                                                                    >  1:2752245/1‑140 (MQ=255)
                                    gcaggTGATCAGAGCGAGCAAATTTTTACCCGCTGGCGTGCTGGGGCGTATAACGCCTGGTCGTTGCCTGGGCGCTGTTTTATCGTTCTGGAGGAGTTGCGCT‑GGGGGGCATTTGGCGATGCCTGCCGTCTGGGAAGccc                                                                                                    >  2:7722701/1‑140 (MQ=255)
                                         tGATCAGAGCGAGCAAATTTTTACCCGCTGGCGTGCTGGGGCGTATAACGCCTGGTCGTTGCCTGGGCGCTGTTTTATCGTTCTGGAGGAGTTGCGCT‑GGGGGGCATTTGGCGATGCCTGCCGTCTGGGAAGCCCGCAAg                                                                                               >  2:895554/1‑140 (MQ=255)
                                         tGATCAGAGCGAGCAAATTTTTACCCGCTGGCGTGCTGGGGCGTATAACGCCTGGTCGTTGCCTGGGCGCTGTTTTATCGTTCTGGAGGAGTTGCGCT‑GGGGGGCATTTGGCGATGCCTGCCGTCTGGGAAGCCCGCAAg                                                                                               >  1:2539736/1‑140 (MQ=255)
                                           aTCAGAGCGAGCAAATTTTTACCCGCTGGCGTGCTGGGGCGTATAACGCCTGGTCGTTGCCTGGGCGCTGTTTTATCGTTCTGGAGGAGTTGCGCT‑GGGGGGCATTTGGCGATGCCTGCCGTCTGGGAAGCCCGCAAGCg                                                                                             >  1:5249537/1‑140 (MQ=255)
                                              agagCGAGCCCATATTTACCCGCAGGCGTGCTGGGCCGTATAACGCCAGGTCGTAGCCTCGGCGCTGGTTTATAGTTCTGGAGGAGTTGCGC‑GGGGGGGCATTTGGCGATGCCTGCCGTCTTGGAAGCCCGCAACCgggg                                                                                          >  2:3071289/1‑138 (MQ=255)
                                              agagCGAGCAAATTTTTACCCGCTGGCGTGCTGGGGCGTATAACGCCTGGTCGTTGCCTGGGCGCTGTTTTATCGTTCTGGAGGAGTTGCGCT‑GGGGGGCATTTGGCGATGCCTGCCGTCTGGGAAGCCCGCAAGCGGTg                                                                                          <  1:2642476/140‑1 (MQ=255)
                                              agagCGAGCAAATTTTTACCCGCTGGCGTGCTGGGGCGTATAACGCCTGGTCGTTGCCTGGGCGCTGTTTTATCGATCTGGAGGAGTTGCGCT‑GGGGGGCATTTGGCGATGCCTGCCGTCTGGGAAGCCCGCAAGCGGTg                                                                                          <  2:1813689/140‑1 (MQ=255)
                                                agcgagcAAATTTTTACCCGCTGGCGTGCTGGGGCGTATAACGCCTGGTCGTTGCCTGGGCGCTGTTTTATCGTTCTGGAGGAGTTGCGCT‑GGGGGGCATTTGGCGATGCCTGCCGTCTGGGAAGCCCGCAAGCGGTGGc                                                                                        >  1:5402508/1‑140 (MQ=255)
                                                 gcgagcAAATTTTTACCCGCTGGCGTGCTGGGGCGTATAACGCCTGGTCGTTGCCTGGGCGCTGTTTTATCGTTCTGGAGGAGTTGCGCT‑GGGGGGCATTTGGCGATGCCTGCCGTCTGGGAAGCCCGCAAGCGGTGGCg                                                                                       <  1:4731773/140‑1 (MQ=255)
                                                  cgagcAAATTTTTACCCGCTGGCGTGCTGGGGCGTATAACGCCTGGTCGTTGCCTGGGCGCTGTTTTATCGTTCTGGAGGAGTTGCGCT‑GGGGGGCATTTGGCGATGCCTGCCGTCTGGGAAGCCCGCAAGCGGTGGCgt                                                                                      >  2:1901918/1‑140 (MQ=255)
                                                     gcAAATTTTTACCCGCTGGCGTGCTGGGGCGTATAACGCCTGGTCGTTGCCTGGGCGCTGTTTTATCGTTCTGGAGGAGTTGCGCT‑GGGGGGCATTTGGCGATGCCTGCCGTCTGGGAAGCCCGCAAGCGGTGGCgttgt                                                                                   >  2:9271841/1‑140 (MQ=255)
                                                     gcAAATTTTTACCCGCTGGCGTGCTGGGGCGTATAACGCCTGGTCGTTGCCTGGGCGCTGTTTTATCGTTCTGGAGGAGTTGCGCT‑GGGGGGCATTTGGCGATGCCTGCCGTCTGGGAAGCCCGCAAGCGGTGGCgttgt                                                                                   >  2:6947861/1‑140 (MQ=255)
                                                       aaaTTTTTACCCGCTGGCGTGCTGGGGCGTATAACGCCTGGTCGTTGCCTGGGCGCTGTTTTATCGTTCTGGAGGAGTTGCGCT‑GGGGGGCATTTGGCGATGCCTGCCGTCTGGGAAGCCCGCAAGCGGTGGCGttgttg                                                                                 >  1:2871157/1‑140 (MQ=255)
                                                        aaTTTTTACCCGCTGGCGTGCTGGGGCGTATAACGCCTGGTCGTTGCCTGGGCGCTGTTTTATCGTTCTGGAGGAGTTGCGCT‑GGGGGGCATTTGGCGATGCCTGCCGTCTGGGAAGCCCGCAAGCGGTGGCGTTGTTGc                                                                                <  1:8021585/140‑1 (MQ=255)
                                                              tACCCGCTGGCGTGCTGGGGCGTATAACGCCTGGTCGTTGCCTGGGCGCTGTTTTATCGTTCTGGAGGAGTTGCGCT‑GGGGGGCATTTGGCGATGCCTGCCGTCTGGGAAGCCCGCAAGCGGTGGCGTTGTTGCTGGGTg                                                                          <  1:9151236/140‑1 (MQ=255)
                                                               aCCCGCTGGCGTGCTGGGGCGTATAACGCCTGGTCGTTGCCTGGGCGCTGTTTTATCGTTCTGGAGGAGTTGCGCT‑GGGGGGCATTTGGCGATGCCTGCCGTCTGGGAAGCCCGCAAGCGGTGGCGTTGTTGCGGGGTGa                                                                         >  2:6623236/1‑140 (MQ=255)
                                                                cccGCTGGCGTGCTGGGGCGTATAACGCCTGGTCGTTGCCTGGGCGCTGTTTTATCGTTCTGGAGGAGTTGCGCT‑GGGGGGCATTTGGCGATGCCTGCCGTCTGGGAAGCCCGCAAGCGGTGGCGTTGTTGCTGGGTGAt                                                                        <  1:1322229/140‑1 (MQ=255)
                                                                    cTGGCGTGCTGGGGGGTTTAGCGCCTGGTCGTTGCCTGGGCGCTGTTTTCTGGTTGTTGAGGAGTTGCGCT‑GGGGGGCGTATGGCGTTGCGTGCCGTCGGGGGAGGCCGCCAGGGGTGGCGTGGTTGCTGGGTggggtgg                                                                    >  2:3251503/1‑134 (MQ=255)
                                                                          tGCTGGGGCGTATAACGCCTGGTCGTTGCCTGGGCGCTGTTTTATCGTTCTGGAGGAGTTGCGCT‑GGGGGGCATTTGGCGATGCCTGCCGTCTGGGAAGCCCGCAAGCGGTGGCGTTGTTGCTGGGTGATTTGCTCgaga                                                              >  1:7423164/1‑140 (MQ=255)
                                                                           gCTGGGGCGTATAACGCCTGGTCGTTGCCTGGGCGCTGTTTTATCGTTCTGGAGGAGTTGCGCT‑GGGGGGCATTTGGCGATGCCTGCCGTCTGGGAAGCCCGCAAGCGGTGGCGTTGTTGCTGGGTGATTTGCTCGAGaa                                                             <  2:2787323/140‑1 (MQ=255)
                                                                            cTGGGGCGTATAACGCCTGGTCGTTGCCTGGGCGCTGTTTTATCGTTCTGGAGGAGTTGCGCT‑GGGGGGCATTTGGCGATGCCTGCCGTCTGGGAAGCCCGCAAGCGGTGGCGTTGTTGCTGGGTGATTTGCTCGAGaaa                                                            >  2:79134/1‑140 (MQ=255)
                                                                             tGGGGCGTATAACGCCTGGTCGTTGCCTGGGCGCTGTTTTATCGTTCTGGAGGAGTTGCGCT‑GGGGGGCATTTGGCGATGCCTGCCGTCTGGGAAGCCCGCAAGCGGTGGCGTTGTTGCTGGGTGATTTGCTCGAGAAAg                                                           <  2:82434/140‑1 (MQ=255)
                                                                               gggCGTATAACGCCTGGTCGTTGCCTGGGCGCTGTTTTATCGTTCTGGAGGAGTTGCGCT‑GGGGGGCATTTGGCGATGCCTGCCGTCTGGGAAGCCCGCAAGCGGTGGCGTTGTTGCTGGGTGATTTGCTCGAGAAAGCg                                                         <  1:86122/140‑1 (MQ=255)
                                                                                  cGTATAACGCCTGGTCGTTGCCTGGGCGCTGTTTTATCGTTCTGGAGGAGTTGCGCT‑GGGGGGCATTTGGCGATGCCTGCCGTCTGGGAAGCCCGCAAGCGGTGGCGTTGTTGCTGGGTGATTTGCTCGAGAAAGCGaca                                                      <  2:4827352/140‑1 (MQ=255)
                                                                                  cGTATAACGCCTGGTCGTTGCCTGGGCGCTGTTTTATCGTTCTGGAGGAGTTGCGCT‑GGGGGGCATTTGGCGATGCCTGCCGTCTGGGAAGCCCGCAAGCGGTGGCGTTGTTGCTGGGTGATTTGCTCGAGAAAGCGaca                                                      <  2:7603795/140‑1 (MQ=255)
                                                                                  cGTATAACGCCTGGTCGTTGCCTGGGCGCTGTTTTATCGTTCTGGAGGAGTTGCGCT‑GGGGGGCATTTGGCGATGCCTGCCGTCTGGGAAGCCCGCAAGCGGTGGCGTTGTTGCTGGGTGATTTGCTCGAGAAAGCGaca                                                      <  2:5063103/140‑1 (MQ=255)
                                                                                  cGTATAACGCCTGGTCGTTGCCTGGGCGCTGTATTATCGTTCTGGAGGAGTTGCGCT‑GGGGGGCATTTGGCGATGCCTGCCGTCTGGGAAGCCCGCAAGCGGTGGCGTTGTTGCTGGGTGATTTGCTCGAGAAAGCGaca                                                      <  2:9211868/140‑1 (MQ=255)
                                                                                    tataACGCCTGGTCGTTGCCTGGGCGCTGTTTTATCGTTCTGGAGGAGTTGCGCT‑GGGGGGCATTTGGCGATGCCTGCCGTCTGGGAAGCCCGCAAGCGGTGGCGTTGTTGCTGGGTGATTTGCTCGAGAAAGCGAcaca                                                    <  2:5909008/140‑1 (MQ=255)
                                                                                      taACGCCTGGTCGTTGCCTGGGCGCTGTTTTATCGTTCTGGAGGAGTTGCGCT‑GGGGGGCATTTGGCGATGCCTGCCGTCTGGGAAGCCCGCAAGCGGTGGCGTTGTTGCTGGGTGATTTGCTCGAGAAAGCGACacaac                                                  <  2:7323954/140‑1 (MQ=255)
                                                                                      caccGCCGGGTCGTGGCCTGGGCGCTGTATGATCGTTCTGCAGGATATGCCCT‑TGGGGGCATGTGGCGCTGCCTGCCGTCTGGGAAGCCCGCAAGCGGTGGCGTTTTTGCTGGCTGATTAGCTCGAGACAGCGACacaac                                                  <  2:5627981/137‑1 (MQ=255)
                                                                                         cGCCTGGTCGTTGCCTGGGCGCTGTTTTATCGTTCTGGAGGAGTTGCGCT‑GGGGGGCATTTGGCGATGCCTGCCGTCTGGGAAGCCCGCAAGCGGTGGCGTTGTTGCTGGGTGATTTGCTCGAGAAAGCGACACAACATc                                               >  1:8301464/1‑140 (MQ=255)
                                                                                           ccTGGTCGTTGCCTGGGCGCTGTTTTATCGTTCTGGAGGAGTTGCGCT‑GGGGGGCATTTGGCGATGCCTGCCGTCTGGGAAGCCCGCAAGCGGTGGCGTTGTTGCTGGGTGATTTGCTCGAGAAAGCGACACAACATCTg                                             >  2:6445321/1‑140 (MQ=255)
                                                                                           ccTGGTCGTTGCCTGGGCGCTGTTTTATCGTTCTGGAGGAGTTGCGCT‑GGGGGGCATTTGGCGATGCCTGCCGTCTGGGAAGCCCGCAAGCGGTGGCGTTGTTGCTGGGTGATTTGCTCGAGAAAGCGACACAACATCTg                                             >  1:4461124/1‑140 (MQ=255)
                                                                                            cTGGTCGTTGCCTGGGCGCTGTTTTATCGTTCTGGAGGAGTTGCGCT‑GGGGGGCATTTGGCGATGCCTGCCGTCTGGGAAGCCCGCAAGCGGTGGCGTTGTTGCTGGGTGATTTGCTCGAGAAAGCGACACAACATCTgg                                            <  1:8808924/140‑1 (MQ=255)
                                                                                            cTGGTCGTTGCCTGGGCGCTGTTTTATCGTTCTGGAGGAGTTGCGCT‑GGGGGGCATTTGGCGATGCCTGCCGTCTGGGAAGCCCGCAAGCGGTGGCGTTGTTGCTGGGTGATTTGCTCGAGAAAGCGACACAACATCTgg                                            <  1:2333914/140‑1 (MQ=255)
                                                                                            cTGGTCGTTGCCTGGGCGCTGTTTTATCGTTCTGGAGGAGTTGCGCT‑GGGGGGCATTTGGCGATGCCTGCCGTCTGGGAAGCCCGCAAGCGGTGGCGTTGTTGCTGGGTGATTTGCTCGAGAAAGCGACACAACATCTgg                                            <  1:3925541/140‑1 (MQ=255)
                                                                                              ggTCGTTGCCTGGGCGCTGTTTTATCGTTCTGGAGGAGTTGCGCT‑GGGGGGCATTTGGCGATGCCTGCCGTCTGGGAAGCCCGCAAGCGGTGGCGTTGTTGCTGGGTGATTTGCTCGAGAAAGCGACACAACATCTGGCa                                          <  1:3563370/140‑1 (MQ=255)
                                                                                              ggTCGTTGCCTGGGCGCTGTTTTATCGTTCTGGAGGAGTTGCGCT‑GGGGGGCATTTGGCGATGCCTGCCGTCTGGGAAGCCCGCAAGCGGTGGCGTTGTTGCTGGGTGATTTGCTCGAGAAAGCGACACAACATCTGGCa                                          >  1:7868611/1‑140 (MQ=255)
                                                                                              ggTCGTTGCCTGGGCGCTGTTTTATCGTTCTGGAGGAGTTGCGCT‑GGGGGGCATTTGGCGATGCCTGCCGTCTGGGAAGCCCGCAAGCGGTGGCGTTGTTGCTGGGTGATTTGCTCGAGAAAGCGACACAACATCTGGCa                                          <  2:9107529/140‑1 (MQ=255)
                                                                                              ggTCGTTGCCTGGGCGCTGTTTTATCGTTCTGGAGGAGTTGCGCT‑GGGGGGCATTTGGCGATGCCTGCCGTCTGGGAAGCCCGCAAGCGGTGGCGTTGTTGCTGGGTGATTTGCTCGAGAAAGCGACACAACATCTGGCa                                          >  2:874511/1‑140 (MQ=255)
                                                                                              ggTCGTTGCCTGGGCGCTGTTTTATCGTTCTGGAGGAGTTGCGCT‑GGGGGGCATTTGGCGATGCCTGCCGTCTGGGAAGCCCGCAAGCGGTGGCGTTGTTGCTGGGTGATTTGCTCGAGAAAGCGACACAACATCTGGCa                                          <  2:5290617/140‑1 (MQ=255)
                                                                                              ggTCGTTGCCTGGGCGCTGTTTTATCGTTCTGGAGGAGTTGCGCT‑GGGGGGCATTTGGCGATGCCTGCCGTCTGGGAAGCCCGCAAGCGGTGGCGTTGTTGCTGGGTGATTTGCTCGAGAAAGCGACACAACATCTGGCa                                          <  1:5537345/140‑1 (MQ=255)
                                                                                              ggTCGTTGCCTGGGCGCTGTTTTATCGTTCTGGAGGAGTTGCGCT‑GGGGGGCATTTGGCGATGCCTGCCGTCTGGGAAGCCCGCAAGCGGTGGCGTTGTTGCTGGGTGATTTGCTCGAGAAAGCGACACAACATCTGGCa                                          <  2:1224625/140‑1 (MQ=255)
                                                                                              ggTCGTTGCCTGGGCGCTGTTTTATCGTTCTGGAGGAGTTGCGCT‑GGGGGGCATTTGGCGATGCCTGCCGTCTGGGAAGCCCGCAAGCGGTGGCGTTGTTGCTGGGTGATTTGCTCGAGAAAGCGACACAACATCTGGCa                                          <  1:6744065/140‑1 (MQ=255)
                                                                                              ggTCGTTGCCTGGGCGCTGTTTTATCGTTCTGGAGGAGTTGCGCT‑GGGGGGCATTTGGCGATGCCTGCCGTCTGGGAAGCCCGCAAGCGGTGGCGTTGTTGCTGGGTGATTTGCTCGAGAAAGCGACACAACATCTGGCa                                          <  1:9313580/140‑1 (MQ=255)
                                                                                              ggTCGTTGCCTGGGCGCAGTTTTATCGTTCTGGAGGAGTTGCGCT‑GGGGGGCATTTGGCGATGCCTGCCGTCTGGGAAGCCCGCAAGCGGTGGCGTTGTTTCGGGGTGATTTGCTCGAGAAAGCGACACAACATCTGGCa                                          >  2:6213532/1‑140 (MQ=255)
                                                                                              ggTCGGTGCCTGGGCGCTGTTTTATCGTTCTGGAGGAGTTGCGCT‑GGGGGGCATTTGGCGAGGCCTGCCGTCTGGGAAGCCCGCAAGCGGTGGCGTTGTTGCTGGGTGATTTGCTCGAGAAAGCGACACAACATCTGGCa                                          <  2:3715502/140‑1 (MQ=255)
                                                                                                    tGCCTGGGCGCTGTTTTATCGTTCTGGAGGAGTTGCGCT‑GGGGGGCATTTGGCGATGCCTGCCGTCTGGGAAGCCCGCAAGCGGTGGCGTTGTTGCTGGGTGATTTGCTCGAGAAAGCGACACAACATCTGGCAGAGAGt                                    >  1:6199308/1‑140 (MQ=255)
                                                                                                    tGCCTGGGCGCTGTTTTATCGTTCTGGAGGAGTTGCGCT‑GGGGGGCATTTGGCGATGCCTGCCGTCTGGGAAGCCCGCAAGCGGTGGCGTTGTTGCTGGGTGATTTGCTCGAGAAAGCGACACAACATCTGGCAGAGAGt                                    <  1:2450644/140‑1 (MQ=255)
                                                                                                    tGCCTGGGCGCTGTTTGATCGTTCTGGAGGAGTTGCGCT‑GGGGGGCATTTGGCGATGCCTGCCGTCTGGGAAGCCCGCAAGCGGTGGCGTTGTTGCTGGGTGATTTGCTCGAGACAGCGACACAACATCTGGCAGAGAGt                                    <  2:4422631/140‑1 (MQ=255)
                                                                                                      ccTGGGCGCTGTTTTATCGTTCTGGAGGAGTTGCGCT‑GGGGGGCATTTGGCGATGCCTGCCGTCTGGGAAGCCCGCAAGCGGTGGCGTTGTTGCTGGGTGATTTGCTCGAGAAAGCGACACAACATCTGGCAGAGAGTAt                                  <  2:4990990/140‑1 (MQ=255)
                                                                                                         gggCGCTGTTTTATCGTTCTGGAGGAGTTGCGCT‑GGGGGGCATTTGGCGATGCCTGCCGTCTGGGAAGCCCGCAAGCGGTGGCGTTGTTGCTGGGTGATTTGCTCGAGAAAGCGACACAACATCTGGCAGAGAGTATCaa                               >  1:8504850/1‑140 (MQ=255)
                                                                                                             gcTGTTTTATCGTTCTGGAGGAGTTGCGCT‑GGGGGGCATTTGGCGATGCCTGCCGTCTGGGAAGCCCGCAAGCGGTGGCGTTGTTGCTGGGTGATTTGCTCGAGAAAGCGACACAACATCTGGCAGAGAGTATCAATGCg                           <  1:1785661/140‑1 (MQ=255)
                                                                                                               tGTTTTATCGTTCTGGAGGAGTTGCGCT‑GGGGGGCATTTGGCGATGCCTGCCGTCTGGGAAGCCCGCAAGCGGTGGCGTTGTTGCTGGGTGATTTGCTCGAGAAAGCGACACAACATCTGGCAGAGAGTATCAATGCGGc                         >  1:1510278/1‑140 (MQ=255)
                                                                                                                gTTTTATCGTTCTGGAGGAGTTGCGCT‑GGGGGGCATTTGGCGATGCCTGCCGTCTGGGAAGCCCGCAAGCGGTGGCGTTGTTGCTGGGTGATTTGCTCGAGAAAGCGACACAACATCTGGCAGAGAGTATCAATGCGGCa                        <  2:2156285/140‑1 (MQ=255)
                                                                                                                      tCGTTCTGGAGGAGTTGCGCT‑GGGGGGCATTTGGCGATGCCTGCCGTCTGGGAAGCCCGCAAGCGGTGGCGTTGTTGCTGGGTGATTTGCTCGAGAAAGCGACACAACATCTGGCAGAGAGTATCAATGCGGCACCGAcc                  <  1:3450384/140‑1 (MQ=255)
                                                                                                                         ttCTGGAGGAGTTGCGCT‑GGGGGGCATTTGGCGATGCCTGCCGTCTGGGAAGCCCGCAAGCGGTGGCGTTGTTGCTGGGTGATTTGCTCGAGAAAGCGACACAACATCTgg                                            >  2:2391716/1‑111 (MQ=255)
                                                                                                                           cTGGAGGAGTTGCGCT‑GGGGGGCATTTGGCGATGCCTGCCGTCTGGGAAGCCCGCAAGCGGTGGCGTTGTTGCTGGGTGATTTGCTCGAGAAAGCGACACAACATCTGGCAGAGAGTATCAATGCGGCACCGACCAcgcg             <  1:8455298/140‑1 (MQ=255)
                                                                                                                           cTGGAGGAGTTGCGCT‑GGGGGGCATTTGGCGATGCCTGCCGTCTGGGAAGCCCGCAAGCGGTGGCGTTGTTGCTGGGTGATTTGCTCGAGAAAGCGACACAACATCTGGCAGAGAGTATCAATGCGGCACCGACCAcgcg             >  1:3129284/1‑140 (MQ=255)
                                                                                                                            tGGAGGAGTTGCGCT‑GGGGGGCATTTGGCGATGCCTGCCGTCTGGGAAGCCCGCAAGCGGTGGCGTTGTTGCTGGGTGATTTGCTCGAGAAAGCGACACAACATCTGGCAGAGAGTATCAATGCGGCACCGACCACGCGt            <  1:8934684/140‑1 (MQ=255)
                                                                                                                            tGGAGGAGTTGCGCT‑GGGGGGCATTTGGCGATGCCTGCCGTCTGGGAAGCCCGCAAGCGGTGGCGTTGTTGCTGGGTGATTTGCTCGAGAAAGCGACACAACATCTGGCAGAGAGTATCAATGCGGCACCGACCACGCGt            <  2:105548/140‑1 (MQ=255)
                                                                                                                            tGGAGGAGTTGCGCT‑GGGGGGCATTTGGCGATGCCTGCCGTCTGGGAAGCCCGCAAGCGGTGGCGTTGTTGCTGGGTGATTTGCTCGAGAAAGCGACACAACATCTGGCAGAGAGTATCAATGCGGCACCGACCACGCGt            <  1:4609325/140‑1 (MQ=255)
                                                                                                                            tGGAGGAGTTGCGC‑GGGGGGGCATTTGGCGATGCCTGCCGTCTGGGAAGCCCGCAAGCGGTGGCGTTGTTGCTGGGTGATTTGCTCGAGAAAGCGACACAACATCTGGCAGAGAGTATCAATGCGGCACCGACCACGCGt            <  2:8319179/140‑1 (MQ=255)
                                                                                                                             ggaggaGTTGCGCT‑GGGGGGCATTTGGCGATGCCTGCCGTCTGGGAAGCCCGCAAGCGGTGGCGTTGTTGCTGGGTGATTTGCTCGAG‑AAGCGACACAACATCTGGCAGAGAGTATCAATGCGGCACCGACCACGCGTCa          <  1:2135645/140‑1 (MQ=255)
                                                                                                                              gaggagTTGCGCT‑GGGGGGCATTTGGCGATGCCTGCCGTCTGGGAAGCCCGCAAGCGGTGGCGTTGTTGCTGGGTGATTTGCTCGAGAAAGCGACACAACATCTGGCAGAGAGTATCAATGCGGCACCGACCACGCGTCa          <  1:6576626/140‑1 (MQ=255)
                                                                                                                              gaggagTTGCGCT‑GGGGGGCATTTGGCGATGCCTGCCGTCTGGGAAGCCCGCAAGCGGTGGCGTTGTTGCTGGGTGATTTGCTCGAGAAAGCGACACAACATCTGGCAGAGAGTATCAATGCGGCACCGACCACGCGTCa          <  1:7303116/140‑1 (MQ=255)
                                                                                                                                ggagTTGCGCT‑GGGGGGCATTTGGCGATGCCTGCCGTCTGGGAAGCCCGCAAGCGGTGGCGTTGTTGCTGGGTGATTTGCTCGAGAAAGCGACACAACATCTGGCAGAGAGTATCAATGCGGCACCGACCACGCGTCACt        <  1:1023362/140‑1 (MQ=255)
                                                                                                                                   gTTGCGCT‑GGGGGGCATTTGGCGATGCCTGCCGTCTGGGAAGCCCGCAAGCGGTGGCGTTGTTGCTGGGTGATTTGCTCGAGAAAGCGACACAACATCTGGCAGAGAGTATCAATGCGGCACCGACCACGCGTCACTAtt     >  1:5107578/1‑140 (MQ=255)
                                                                                                                                    ttGCGCT‑GGGGGGCATTTGGCGATGCCTGCCGTCTGGGAAGCCCGCAAGCGGTGGCGTTGTTGCTGGGTGATTTGCTCGAGAAAGCGACACAACATCTGGCAGAGAGTAt                                  <  1:3778826/110‑1 (MQ=255)
                                                                                                                                    ttGCGCT‑GGGGGGCATTTGGCGATGCCTGCCGTCTGGGAAGCCCGCAAGCGGTGGCGTTGTTGCTGGGTGATTTGCTCGAGAAAGCGACACAACATCTGGCAGAGAGTATCAATGCGGCACCGACCACGCGTCACTATTa    >  2:3541075/1‑140 (MQ=255)
                                                                                                                                     tGCGCT‑GGGGGGCATTTGGCGATGCCTGCCGTCTGGGAAGCCCGCAAGCGGTGGCGTTGTTGCTGGGTGATTTGCTCGAGAAAGCGACACAACATCTGGCAGAGAGTATCAATGCGGCACCGACCACGCGTCACTATTAc   <  1:4323719/140‑1 (MQ=255)
                                                                                                                                      gcgcT‑GGGGGGCATTTGGCGATGCCTGCCGTCTGGGAAGCCCGCAAGCGGTGGCGTTGTTGCTGGGTGATTTGCTCGAGAAAGCGACACAACATCTGGCAGAGAGTATCAATGCGGCACCGACCACGCGTCACTATTAcc  >  1:7228105/1‑140 (MQ=255)
                                                                                                                                      gcgcT‑GGGGGGCATTTGGCGATGCCTGCCGTCTGGGAAGCCCGCAAGCGGTGGCGTTGTTGCTGGGTGATTTGCTCGAGAAAGCGACACAACATCTGGCAGAGAGTATCAATGCGGCACCGACCACGCGTCACTATTAcc  <  2:8829466/140‑1 (MQ=255)
                                                                                                                                           |                                                                                                                                       
GAAAAGGCGTTAACGCAACGAGGCCTACTGGTGCAGGCAGGTGATCAGAGCGAGCAAATTTTTACCCGCTGGCGTGCTGGGGCGTATAACGCCTGGTCGTTGCCTGGGCGCTGTTTTATCGTTCTGGAGGAGTTGCGCTGGGGGGGCATTTGGCGATGCCTGCCGTCTGGGAAGCCCGCAAGCGGTGGCGTTGTTGCTGGGTGATTTGCTCGAGAAAGCGACACAACATCTGGCAGAGAGTATCAATGCGGCACCGACCACGCGTCACTATTACC  >  NZ_CP025268/4331675‑4331949

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑