BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP025268	1,374,862	(T)_8→9	intergenic (+40/‑172)	pspE → / → ycjM	thiosulfate sulfurtransferase PspE/glucosylglycerate phosphorylase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP025268	1,374,854	1	.	T	94.0%	1550.7 / 89.8	508	intergenic (+32/‑180)	pspE/ycjM	thiosulfate sulfurtransferase PspE/glucosylglycerate phosphorylase
Reads supporting (aligned to +/- strand): ref base . (17/13); new base T (258/212); total (275/233)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.05e-01

Only 100 of 534 total aligned reads displayed.

GGACGCCAGTCAGGGCAAGCAAAAGAGATCCTTAGCGAGATGGGATATACCCACGTTGAGAACGCCGGTGGCCTGAAAGACATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGC‑C‑G‑T‑T‑TTTTTTCTGTCACACCTTATTTACATCCCCATAGATTATTTGCGTCAGCTCACAAATACGCTTTTTCCCTGGTAAAAAATGATTTCCTGCGTGACTAAAACCCTTGTGCTCAATTGACAGTTTATTTTCTG  >  NZ_CP025268/1374715‑1374987

      cAGTCAGGGCAAGCAAAAGAGATCCTTAGCGAGATGGGATATACCCACGTTGAGAACGCCGGTGGCCTGAAAGACATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGC‑C‑G‑t‑t‑tttt                                                                                                                                 >  1:7332755/1‑140 (MQ=255)

      cAGTCAGGGCAAGCAAAAGAGATCCTTAGCGAGATGGGATATACCCACGTTGAGAACGCCGGTGGCCTGAAAGACATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGC‑C‑G‑t‑t‑tttt                                                                                                                                 >  1:1016589/1‑140 (MQ=255)

              gcaagcaaAAGAGATCCTTAGCGAGATGGGATATACCCACGTTGAGAACGCCGGTGGCCTGAAAGACATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGC‑C‑GTT‑T‑TTTTTTCTGTc                                                                                                                          >  2:415355/1‑140 (MQ=255)

               caagcaaAAGAGATCCTTAGCGAGATGGGATATACCCACGTTGAGAACGCCGGTGGCCTGAAAGACATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGC‑C‑GTT‑T‑TTTTTTCTGTca                                                                                                                         <  2:8846183/140‑1 (MQ=255)

               caagcaaAAGAGATCCTTAGCGAGATGGGATATACCCACGTTGAGAACGCCGGTGGCCTGAAAGACATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGC‑C‑G‑T‑T‑TTTTTTCTGTcac                                                                                                                        <  1:518939/140‑1 (MQ=255)

               caagcaaAAGAGATCCTTAGCGAGATGGGATATACCCACGTTGAGAACGCCGGTGGCCTGAAAGACATCGCAATGCCGAAGGTAAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGC‑C‑GTT‑T‑TTTTTTCTGTca                                                                                                                         <  2:2028769/140‑1 (MQ=255)

               caagaaaAAGATATCCTTAGCGAGATGGGATATACCCACGTTGAGAACGCCGGTGGCCTGAAATACAACGCAATGCCGAAGGTAAAAGTTTAAAAGAATTCACCATGAGCGGCGCTTATGCGC‑C‑GTT‑T‑TTTTTTCTGTca                                                                                                                         <  2:1586061/140‑1 (MQ=255)

                aagcaaACGAGATCCTTAGCGAGATGGGATATACCCACGTTGTGAACGCCGGTGGCCTGAAAGACATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGC‑C‑GTT‑T‑TTTTTTCTGTcac                                                                                                                        <  2:2981973/140‑1 (MQ=255)

                aagcaaAAGAGATCCTTAGCGAGATGGGATATACCCACGTTGAGAACGCCGGTGGCCTGAAAGACATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGC‑C‑GTT‑T‑TTTTTTCTGTcac                                                                                                                        <  1:6176977/140‑1 (MQ=255)

                aagcaaAAGAGATCCTTAGCGAGATGGGATATACCCACGTTGAGAACGCCGGTGGCCTGAAAGACATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGC‑C‑GTT‑T‑TTTTTTCTGTcac                                                                                                                        <  1:1963092/140‑1 (MQ=255)

                aagcaaAAGAGATCCTTAGCGAGATGGGATATACCCACGTTGAGAACGCCGGTGGCCTGAAAGACATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGC‑C‑GTT‑T‑TTTTTTCTGTcac                                                                                                                        <  1:5644759/140‑1 (MQ=255)

                aagcaaAAGAGATCCTTAGCGAGATGGGATATACCCACGTTGAGAACGCCGGTGGCCTGAAAGACATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGC‑C‑GTT‑T‑TTTTTTCTGTcac                                                                                                                        <  1:8460214/140‑1 (MQ=255)

                aagcaaAAGAGATCCTTAGCGAGATGGGATATACCCACGTTGAGAACGCCGGTGGCCTGAAAGACATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGC‑C‑GTT‑T‑TTTTTTCTGTcac                                                                                                                        <  1:3895950/140‑1 (MQ=255)

                aagcaaAAGAGATCCTTAGCGAGATGGGATATACCCACGTTGAGAACGCCGGTGGCCTGAAAGACATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGC‑C‑GTT‑T‑TTTTTTCTGTcac                                                                                                                        <  2:2553388/140‑1 (MQ=255)

                 agcaaAAGAGATCCTTAGCGAGATGGGATATACCCACGTTGAGAACGCCGGTGGCCTGAAAGAAATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGC‑C‑GTT‑T‑TTTTTTCTGTcaca                                                                                                                       <  2:4012294/140‑1 (MQ=255)

                  gcaaAAGAGATCCTTAGCGAGATGGGATATACCCACGTTGAGAACGCCGGTGGCCTGAAAGACATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGC‑C‑GTT‑T‑TTTTTTCTGTCacac                                                                                                                      >  1:8830188/1‑140 (MQ=255)

                   caaAAGAGATCCTTAGCGAGATGGGATATACCCACGTTGAGAACGCCGGTGGCCTGAAAGACATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGC‑C‑GTT‑T‑TTTTTTCTGTCACAcc                                                                                                                     <  1:3389869/140‑1 (MQ=255)

                   caaAAGAGATCCTTAGCGAGATGGGATATACCCACGTTGAGAACGCCGGTGGCCTGAAAGACATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGC‑C‑GTT‑T‑TTTTTTCTGTCACAcc                                                                                                                     <  2:3574189/140‑1 (MQ=255)

                   caaAAGAGATCCTTAGCGAGATGGGATATACCCACGTTGAGAACGCCGGTGGCCTGAAAGACATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGC‑C‑GTT‑T‑TTTTTTCTGTCACAcc                                                                                                                     <  2:2701789/140‑1 (MQ=255)

                   caaAAGAGATCCTTAGCGAGATGGGATATACCCACGTTGAGAACGCCGGTGGCCTGAAAGACATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGC‑C‑GTT‑T‑TTTTTTCTGTCACAcc                                                                                                                     <  1:1326160/140‑1 (MQ=255)

                          gaTCCTTAGCGAGATGGGATATACCCACGTTGAGAACGCCGGTGGCCTGAAAGACATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGC‑C‑GTT‑T‑TTTTTTCTGTCACACCTTATTTa                                                                                                              <  2:4697447/140‑1 (MQ=255)

                          gaTCCTTAGCGAGATGGGATATACCCACGTTGAGAACGCCGGTGGCCTGAAAGACATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGC‑C‑GTT‑T‑TTTTTTCTGTCACACCTTATTTa                                                                                                              <  1:9235562/140‑1 (MQ=255)

                           aTCCTTAGCGAGATGGGATATACCCACGTTGAGAACGCCGGTGGCCTGAAAGACATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGC‑C‑GTT‑T‑TTTTTTCTGTCACACCTTATTTAc                                                                                                             >  1:1841084/1‑140 (MQ=255)

                           aTCCTTAGCGAGATGGGATATACCCACGTTGAGAACGCCGGTGGCCTGAAAGACATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGC‑C‑G‑T‑T‑TTTTTTCTGTCACACCTTATTTACa                                                                                                            <  2:6335428/140‑1 (MQ=255)

                            tCCTTAGCGAGATGGGATATACCCACGTTGAGTACGCCGGTGGCCTGAAAGACATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGC‑C‑GTT‑T‑TTTTTTCTGTCACACCTTATTTACa                                                                                                            <  1:2945420/140‑1 (MQ=255)

                            tCCTTAGCGAGATGGGATATACCCACGTTGAGAACGCCGGTGGCCTGAAAGACATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGC‑C‑GTT‑T‑TTTTTTCTGTCACACCTTATTTACa                                                                                                            <  1:4037506/140‑1 (MQ=255)

                            tCCTTAGCGAGATGGGATATACCCACGTTGAGAACGCCGGTGGCCTGAAAGACATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGC‑C‑GTT‑T‑TTTTTTCAGTGACACCGTAttttaa                                                                                                            >  2:446159/1‑137 (MQ=255)

                             ccTTAGCGAGATGGGATATACCCACGTTGAGAACGCCGGTGGCCTGAAAGACATCGCAATGCCGAAGGACAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGC‑C‑GTT‑T‑TTTTTTCTGTCACACCTTATTTACAt                                                                                                           >  2:5164390/1‑140 (MQ=255)

                                tAGCGAGATGGGATATACCCACGTTGAGAACGCCGGTGGACTGAAAGACATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGC‑C‑GTT‑T‑TTTTTTCTGTCACACCTTATTTACATacc                                                                                                        >  2:9178561/1‑140 (MQ=255)

                                  tcGAGATGGGATATACCCACGTTGAGAACGCCGGTGGCCTGAAAGACATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCTCTTATGCGC‑C‑GTT‑T‑TTTTTTCTGTCACACCTTATTTACATCCCCa                                                                                                      <  2:8652713/139‑1 (MQ=255)

                                  gCGAGATGGGATATACCCACGTTGAGAACGCCGGTGGCCTGAAAGACATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGC‑C‑GTT‑T‑TTTTTTCTGTCACACCTTATTTACATCCCCa                                                                                                      <  2:6184468/140‑1 (MQ=255)

                                  gCGAGATGGGATATACCCACGTTGAGAACGCCGGTGGCCTGAAAGACATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGC‑C‑GTT‑T‑TTTTTTCTGTCACACCTTATTTACATCCCCa                                                                                                      <  1:2236211/140‑1 (MQ=255)

                                   cGAGATGGGATATACCCACGTTGAGAACGCCGGTGGCCTGAAAGACATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGC‑C‑GTT‑T‑TTTTTTCTGTCACACCTTATTTACATCCCCAt                                                                                                     >  2:5554150/1‑140 (MQ=255)

                                         gggATATACCCACGTTGAGAACGCCGGTGGCCTGAAAGACATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGC‑C‑GTT‑T‑TTTTTTCTGTCACACCTTATTTACATCCCCATAGatta                                                                                               <  1:4945884/140‑1 (MQ=255)

                                          ggATATACCCACGTTGAGAACGCCGGTGGCCTGAAAGACCTCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGC‑C‑GTT‑T‑TTTTTTCTGTCACACCTTATTTACATCCCCATAGattat                                                                                              <  2:66874/140‑1 (MQ=255)

                                           gATATACCCACGTTGAGAACGCCGGTGGCCTGAAAGACATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGC‑C‑GTT‑T‑TTTTTTCTGTCACACCTTATTTACATCCCCATAGattatt                                                                                             >  2:3970667/1‑140 (MQ=255)

                                                aCCCACGTTGAGAACGCCGGTGGCCTGAAAGACATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGC‑C‑GTT‑T‑TTTTTTCTGTCACACCTTATTTACATCCCCATAGATTATTTGCGt                                                                                        <  1:6897927/140‑1 (MQ=255)

                                                aCCCACGTTGAGAACGCCGGTGGCCTGAAAGACATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGC‑C‑GTT‑T‑TTTTTTCTGTCACACCTTATTTACATCCCCATAGATTATTTGCGt                                                                                        <  1:2727055/140‑1 (MQ=255)

                                                aCCCACGTTGAGAACGCCGGTGGCCTGAAAGACATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGC‑C‑GTT‑T‑TTTTTTCTGTCACACCTTATTTACATCCCCATAGATTATTTGCGt                                                                                        <  1:8325218/140‑1 (MQ=255)

                                                aCCCACGTTGAGAACGCCGGTGGCCTGAAAGACATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGC‑C‑GTT‑T‑TTTTTTCTGTCACACCTTATTTACATACCCATAGTTTATTTGCGt                                                                                        >  1:109408/1‑140 (MQ=255)

                                                aCCCACGTTGAGAACGCCGGTGGCCTGAAAGACATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGC‑C‑GTT‑T‑TTTTTTATGGCACACCTTATTTACATCCCCATAGATTATTTGCGt                                                                                        >  1:1245502/1‑140 (MQ=255)

                                                  ccACGTTGAGAACGCCGGTGGCCTGAAAGACATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGC‑C‑GTT‑T‑TTTTTTCTGTCACACCTTATTTACATCCCCATAGATTATTTGCGTCa                                                                                      >  2:905188/1‑140 (MQ=255)

                                                   cACGTTGAGAACGCCGGTGGCCTGAAAGACATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGC‑C‑GTT‑T‑TTTTTTCTGTCACACCTTATTTACATCCCCATAGATTATTTGCGTCAg                                                                                     <  1:6797283/140‑1 (MQ=255)

                                                    aCGTTGAGAACGCCGGTGGCCTGAAAGACATCGCAATGCCGAAGGTCAAAGGTTAAAAGACTTCACCATGAGCGGCGCTTATGCGC‑CGG‑T‑T‑TTTTTTCTGTCACACCTTATTTACATCCCCATCGATTATTTGCGTCAGc                                                                                    >  2:373528/1‑140 (MQ=255)

                                                    aCGTTGAGAACGCCGGTGGCCTGAAAGACATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGC‑C‑GTT‑T‑TTTTTTCTGTCACACCTTATTTACATCCCCATTGATTATTTGCGTCAGc                                                                                    >  2:3874630/1‑140 (MQ=255)

                                                    aCGTTGAGAACGCCGGTGGCCTGAAAGACATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGC‑C‑GTT‑T‑TTTTTTCTGTCACACCTTATGTACATCCCCATAGATTATTTGCGTCAGc                                                                                    >  2:6082473/1‑140 (MQ=255)

                                                     ctttGATAACGCGGGTGGCCTTAAAGACATCGCATTGCCTATGTTAAATGTTTAAAATATTTAACAATGAGCGGCGATTAGGGGCGT‑T‑T‑T‑TTTTTTCTGTCACACCTTATTTACATCCCCATAGATTATTTGCGTCAGCt                                                                                   <  2:9301386/138‑1 (MQ=255)

                                                     cGTTGAGAACGCCGTTGGCCTGAAAGACATCGCAATGCCTAAGGTCAAAGGTTAAAAGAATTCACCATGAGCTGCGCTTATGCGA‑C‑G‑TAT‑ATTTTTCTTTAAAAACTTATTTACATACCCATATATTATTTTCGTAAgga                                                                                   >  2:3309755/1‑138 (MQ=255)

                                                     cGTTGAGAACGCCGGTGGCCTGAAAGACATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGC‑C‑GTT‑T‑TTTTTTCTGTCACACCTTATTTACATCCCCATAGATTATTTGCGTCAGCt                                                                                   <  1:9038666/140‑1 (MQ=255)

                                                     cGTTGAGAACGCCGGTGGCCTGAAAGACATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGC‑C‑GTT‑T‑TTTTTTCTGTCACACCTTATTTACATCCCCATAGATTATTTGCGTCAGCt                                                                                   >  2:8381327/1‑140 (MQ=255)

                                                     cGTTGAGAACGCCGGTGGCCTGAAAGACATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGC‑C‑GTT‑T‑TTTTTTCTGTCACACCTTATTTACATCCCCATAGATTATTTGCGTCAGCt                                                                                   >  2:1326902/1‑140 (MQ=255)

                                                     cGTTGAGAACGCCGGTGGCCTGAAAGACATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGC‑C‑GTT‑T‑TTTTTTCTGTCACACCTTATTTACATCCCCATAGATTATTTGCGTCAGCt                                                                                   >  2:1946971/1‑140 (MQ=255)

                                                     cGTTGAGAACGCCGGTGGCCTGAAAGACATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGC‑C‑GTT‑T‑TTTTTTCTGTCACACCTTATTTACATCCCCATAGATTATTTGCGTCAGCt                                                                                   >  1:8876416/1‑140 (MQ=255)

                                                     cGTTGAGAACGCCGGTGGCCTGAAAGACATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGC‑C‑GTT‑T‑TTTTTTATGTCACACCTTATTTACATCCCCATAGATTATTTGCGTCAGCt                                                                                   >  2:2376060/1‑140 (MQ=255)

                                                     cGTTGAGAACGCCGGTGGCCTGAAAGACATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGC‑C‑GTT‑T‑TTTTTTATGTAACACCATATTTACATCCCCAAAGATTATTTTCGTCAGcg                                                                                   >  1:2070903/1‑139 (MQ=255)

                                                       ttGAGAACGCCGGTGGCCTGAAAGACATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGC‑C‑GTT‑T‑TTTTTTCTGTCACACCTTATTTACAACCCCATAGATTATTTTCGTCAGCTca                                                                                 >  1:8129548/1‑140 (MQ=255)

                                                         gagaACGCCGGTGGCCTGAAAGACATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGC‑C‑GTT‑T‑TTTTTTCTGTCACACCTTATTTACATCCCCATAGATTATTTGCGTCAGCTcaca                                                                               >  2:8086071/1‑140 (MQ=255)

                                                          agaACGCCGGTGGCCTGAAAGACATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGC‑C‑GTT‑T‑TTTTTTCTGTCACACCTTATTTACAGACCCATTGATTCTTTGCGTCAGCTCACaa                                                                              >  1:239690/1‑140 (MQ=255)

                                                            aaCGCCGGTGGCCTGAAAGACATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGC‑C‑GTT‑T‑TTTTTTCTGTCACACCTTATTTACATACCCCTAGATTATTTTCGTCAGCTCACAAAt                                                                            >  1:4694906/1‑140 (MQ=255)

                                                               gCCGGTGGCCTGAAAGACATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGC‑C‑GTT‑T‑TTTTTTCTGTCACACCTTATTTTCATACCCCTAGATTATTTGCGTCAGCTCACAAAAACg                                                                         >  1:7164918/1‑140 (MQ=255)

                                                                ccGGTGGCCTGAAAGACATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGC‑C‑GTT‑T‑TTTTTTCTGTCACACCTTATTTACATCCCCAAAGATTATTTGCGTCAGCTCACAAATAAGc                                                                        >  1:3577815/1‑140 (MQ=255)

                                                                    tGGCCTGAAAGACATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGC‑C‑GTT‑T‑TTTTTTATGTCACCCCTTATTTAAATACCCCTAGATTATTTTCGTCAGCTCAAAAATACGCattt                                                                    >  1:5467884/1‑140 (MQ=255)

                                                                       ccTGAAAGACAACGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGC‑C‑GTT‑T‑TTTTTTCTGTCACACCTTATTTACATCCCCATAGATTATTTGCGTCAGCTCACAAATACGCTTTTTcc                                                                 <  1:513673/140‑1 (MQ=255)

                                                                       ccTGAAAGACAACGCAATGCCGAAGGTCAAAGGTTAAAACAATTAACCATGAGCGTCGCTTATGCGC‑C‑GTT‑T‑TTTTTTCTGTCACACCTTATTTACATCCCCATAGATTATTTGCGTCAGCTCACAAAGACGCTTTTTcc                                                                 <  2:1773091/140‑1 (MQ=255)

                                                                         tGAAAGACATCGCAATGCCGAAGGTCAAAGGTTAACAGAATTCACCATGAGCGGCGCTTATGCGC‑C‑GTT‑T‑TTTTTTCTGTCACACCTTATTTACATCCCCATAGATTATTTGCGTCAGCTCACAAATACGCTTTTTCCCt                                                               <  1:6438085/140‑1 (MQ=255)

                                                                         tGAAAGACATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGC‑C‑GTT‑T‑TTTTTTCTGTCACACCTTATTTACATCCCCATAGATTATTTGCGTCAGCTCACAAATACGCTTTTTCCCt                                                               >  2:577595/1‑140 (MQ=255)

                                                                             aGACATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGC‑C‑GTT‑T‑TTTTTTCTGTCACACCTTATTTACATCCCCATAGATTATTTGCGTCAGCTCACAAATACGCTTTTTCCCTGGTa                                                           <  2:6565694/140‑1 (MQ=255)

                                                                               aCATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGC‑C‑GTT‑T‑TTTTTTCTGTCACACCTTATTTACATCCCCATAGATTATTTGCGTCAGCTCACAAATACGCTTTTTCCCTGGTaaa                                                         <  2:7714897/140‑1 (MQ=255)

                                                                               aCATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGC‑C‑GTT‑T‑TTTTTTCTGTCACACCTTATTTACATCCCCATAGATTATTTGCGTCAGCTCACAAATACGCTTTTTCCCTGGTaaa                                                         <  2:7332755/140‑1 (MQ=255)

                                                                                cATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGC‑C‑GTT‑T‑TTTTTTCTGTCACACCTTATTTACATCCCCATAGATTATTTTCGTCAGCTCACAAATACGCTTTTTCCCTGGTaaaa                                                        >  1:8708044/1‑140 (MQ=255)

                                                                                cATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGC‑C‑GTT‑T‑TTTTTTCTGTCACACCTTATTTACATACCCATAGATTATTTGCGTCAGCTCACAAATACGCTTTTTCCCTGGTaaaa                                                        >  1:755117/1‑140 (MQ=255)

                                                                                 aTCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGC‑C‑GTT‑T‑TTTTTTGTGTGACACCGTATTTAAAAACCCAAATATTATTTTCGTCAGCGCACACAAAAGCTTTTTAACTGGGtaaaa                                                       >  1:8268959/1‑140 (MQ=255)

                                                                                 aTCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGC‑C‑GTT‑T‑TTTTTTATGTGAAACCTTATTTAAATACCCATAGATTATTTGCGGCCGCGCACAAATAAGCTTTTTCCCAGGTaaaaa                                                       >  1:4260544/1‑140 (MQ=255)

                                                                                 aTCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGC‑C‑GTT‑T‑TTTTTTATGTCACCCCTTATTTACATCCCCATAGATTATTTGCGGCAGCTCACCAATACGCTTTTTCCCTGGTaaaaa                                                       >  1:5862326/1‑140 (MQ=255)

                                                                                   cGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGC‑C‑GTT‑T‑TTTTTTCTGTAACACCTTATTTACATCCCCATAGATTATTTGCGTCAGCTCACAAATACGCTTTTTCCCTGGTAAAAAAt                                                     >  2:1674047/1‑140 (MQ=255)

                                                                                   cGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGC‑C‑GTT‑T‑TTTTTTCTGTAACACCATATTTAAAAACCCAAAGATTATTTGCGTCAGCTCACAAAAACGCATTTTAACGGGTAAAAAAt                                                     >  1:25444/1‑140 (MQ=255)

                                                                                          ccGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGC‑C‑GTT‑T‑TTTTTTATTTGACAACTTATTTAAATCCCCATAGATTATTTTCGTCAGCACACAAAAACGCATTTTACCTGGTAAAAAATGAtttac                                              >  1:3700278/1‑138 (MQ=255)

                                                                                             aaGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGC‑C‑GTT‑T‑TTTTTTCTGTCACACCGTATTTACATCCCCATAGATTATTTGCGTCAGCTCACAAATACGCTTTTTCCCTGGGAAAAAATGATTTCCTGc                                           >  1:7940453/1‑140 (MQ=255)

                                                                                              agttCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTGATGCGC‑C‑GAT‑T‑TTTTTTCTGTCACACCTTATTTACATCCCCATAGATTATTTGCGTCAGCTCACAAATACGCTTTTTCCCTGGTAAAAAATGATTTCCTGCg                                          <  2:5914210/137‑1 (MQ=255)

                                                                                              aGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGC‑C‑GTT‑T‑TTTTTTCTGTCACACCTTATTTACATCCCCATAGATTATTTGCGTCAGCTCACAAATACGCTTTTTCCCTGGTAAAAAATGATTTCCTGCg                                          <  1:1546297/140‑1 (MQ=255)

                                                                                               ggTCAAAGCTTAAAAGAATTCACCATGAGCGGCGCTTATTCGC‑C‑G‑T‑TATTTTTTCTGTAACACCTTATTTACATCCCCATATATTATTTGCGTCAGCTCACAAATACGCTTTTTCCCTGGTAAAAAATGATTTCCTGCGt                                         <  2:4859378/140‑1 (MQ=255)

                                                                                                  cAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGC‑C‑GTT‑T‑TTTTTTCTGTCACACCTTATTTACATCCCCATAGATTATTTGCGTCAGCTCAACAATACGCTTTTTCCCTGGTAAAAAATGATTTCCTGAGTGAc                                      >  2:6990737/1‑140 (MQ=255)

                                                                                                  cAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGC‑C‑GTT‑T‑TTTTTTCTGTCACACCTTATTTACATACCCATAGATTATTTGCGTCAGCTCACAAAAACGCTTTTTCCCTGGTAAAAAATGATTTACTGCGTGAc                                      >  1:8620244/1‑140 (MQ=255)

                                                                                                  cAAAGGTTAAAAGAATACACCATGAGCGGCGCTTATGCGC‑C‑GTT‑T‑TTTTTTCTGTCACACCTTATTTACATCCCCATAGATTATTTGCGTCAGCTCACAAATACGCTTTTTCCCTGGTAAAAAATGATTTCCTGCGTGAc                                      <  1:6580342/140‑1 (MQ=255)

                                                                                                   aaaGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGC‑C‑GTT‑T‑TTTTTTCTGTCACACCGTATTTAAATACCCATAGATTATTTGCGTCAGCTCACAAAAAACCGTTTTCCCTGGTAAAAAATGTTTTCCTGCGTGACt                                     >  1:1506329/1‑140 (MQ=255)

                                                                                                    aaGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGC‑C‑GTT‑T‑TTTTTTATGTCACACCTTATTTACATCCCCATAGATTATTTGCGTCAGCTCACAAATACGCTTTTTCCCTGGTAAAAAATGATTTCCTGCGTGACTa                                    >  1:1678417/1‑140 (MQ=255)

                                                                                                          aaaaGAATTCACCATGAGCGGCGCTTATGCGC‑C‑GTT‑T‑TTTTTTCTTTCACACCTTATTTACATACCCAAAGATTATTTGCGTCAGCTCACAAAAACGCTTTTTACCAGGTAAAAAATTATTTAAAGCGTGTCTAAAAacc                              >  1:318537/1‑140 (MQ=255)

                                                                                                          aaaaGAATTCACCATGAGCGGCGCTTATGCGC‑C‑GTT‑T‑TTTTTTCTGTAACACCTTATTTACATCCCCATAGATTATTTGCGTCAGCTCCCCAATACGCTTTTTCCCTGGTAAAAAATGTTTTGCTGCGTGACTAAAAccc                              >  2:5311342/1‑140 (MQ=255)

                                                                                                           aaaGAATTCACCATGAGCGGCGCTTATGCGC‑C‑GTT‑T‑TTTTTTATGTCACACCATATTTACATCCCCATAGATTATTTGCGTCAGCTCACAAATAAGCTTTTTACCTGGTAAAAAATGTTTTACTGCGTGTATAAAACCCt                             >  2:5963570/1‑140 (MQ=255)

                                                                                                               aaTTCACCATGAGCGGCGCTTATGCGC‑C‑GTT‑T‑TTTTTTCTGTCACACCTTATTTACATCCCCATAGATTATTTGCGTCAGCTCACAAATACGCTTTTTCCCTGGTAAAAAATGATTTCCTGCGTGACTAAAACCCTtgtg                         <  1:7662504/140‑1 (MQ=255)

                                                                                                                aTTCACCATGAGCGGCGCTTATGCGC‑C‑GTT‑T‑TTTTTTCTGTCACACCTTATTTACATCCCCATAGATTATTTGCGTCAGCTCACAAATACGCTTTTTCCCTGGTAAAAAATGATTTCCTGCGTGACTAAAACCCTTGTGc                        <  2:1123255/140‑1 (MQ=255)

                                                                                                                 ttCACCATGAGCGGCGCTTATGCGC‑C‑GTT‑T‑TTTTTTCTGTCACACCTTATTTACATCCCCa                                                                                                      >  2:2239771/1‑61 (MQ=255)

                                                                                                                 ttCACCATGAGCGGCGCTTATGCGC‑C‑GTT‑T‑TTTTTTCTGTCACACCTTATTTACATCCCCATAGATTATTTGCGTCAGCTCACAAATACGCTTTTTCCCTGGTAAAAAATGATTTCCTGCGTGACTAAAACCCTTGTGCt                       >  2:8015171/1‑140 (MQ=255)

                                                                                                                   cACCATGAGCGGCGCTTATGCGC‑C‑GTT‑T‑TTTTTTCTGTCACACCTTATTTACATCCCCATAGATTATTTGAGTCAGCTCACAAATACGCTTTTTCCCTGGTAAAAAATGATTTCCTGCGTGACTAAAACCCTTGTGCTCa                     <  2:762001/140‑1 (MQ=255)

                                                                                                                      cATGAGCGGCGCTTATGCGC‑C‑GTT‑T‑TTTTTTATTTCACACCTTATTTAAATACCCAAAGATTATTTTAGAAAGATCACAAATAAGCTTTTTACCTGGTAAAAAATGATTTACTTCGTGACAAAAAACCAAGTGCTCAAtt                  >  1:672210/1‑140 (MQ=255)

                                                                                                                         gAGCGGCGCTTATGCGC‑C‑GTT‑T‑TTTTTTCTGTCACACCTTATTTACATCCCCATAGATTATTTGCGTCAGCTCACAAATACGCTTTTTCCCTGGTAAAAAATGATTTACTGCGTGACTAAATACCTTGTGCTCAATTGAc               >  2:7503520/1‑140 (MQ=255)

                                                                                                                              gcgcTTATGCGC‑C‑GTT‑T‑TTTTTTCTTTAACACCATATTTAAATACCCAAAAAATATTTTCGTCAGCGCACAAAAAAGCGTTTTCCCGGGTAAAAAAAGATTTACTGCGTGACTAAAAACCAAGTGCTAAAATTACAGttt          >  1:97761/1‑140 (MQ=255)

                                                                                                                              gcgcTTATGCGC‑C‑GTT‑T‑TTTTTTCTGTCACACCTTATTTACATCCCCATAGATTATTTGCGTCAGCTCACAAATACGCTTTTTCCCTGGTAAAAAATGATTTACTGCGTGACTAAAACCCTTGTGCTCAATTGACAGttt          >  2:690141/1‑140 (MQ=255)

                                                                                                                               cgcTTATGCGC‑C‑GTT‑T‑TTTTTTCAGTCAAACCTTATTTAAATCCCCATAGATTATTTGAGTCAGCTAACAAATACGCATTTTACCTTGTAAAAAATTATTTACTGCGTGACTAAAACCCTTGTGCTCAATTGACAGTTTa         >  2:4390272/1‑140 (MQ=255)

                                                                                                                                   tATGCGC‑C‑GTT‑T‑TTTTTTCTGTCACACCTTATTTACATCCCCATAGATTATTTGCGTCAGCTCACAAATACGCTTTTTCCCTGGTAAAAAATGATTTCCTGCGTGACTAAAACCCTTGTGCTCAATTGACAGTTTAtttt     <  2:3700278/140‑1 (MQ=255)

GGACGCCAGTCAGGGCAAGCAAAAGAGATCCTTAGCGAGATGGGATATACCCACGTTGAGAACGCCGGTGGCCTGAAAGACATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGC‑C‑G‑T‑T‑TTTTTTCTGTCACACCTTATTTACATCCCCATAGATTATTTGCGTCAGCTCACAAATACGCTTTTTCCCTGGTAAAAAATGATTTCCTGCGTGACTAAAACCCTTGTGCTCAATTGACAGTTTATTTTCTG  >  NZ_CP025268/1374715‑1374987

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`