BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP025268	1,701,269	+A	intergenic (‑380/+10)	uidA ← / ← uidR	beta‑glucuronidase/DNA‑binding transcriptional regulator UidR

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP025268	1,701,265	1	.	A	92.2%	2058.0 / 139.8	569	intergenic (‑376/+14)	uidA/uidR	beta‑glucuronidase/DNA‑binding transcriptional regulator UidR
Reads supporting (aligned to +/- strand): ref base . (25/19); new base A (266/256); total (292/277)
Fisher's exact test for biased strand distribution p-value = 5.31e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 597 total aligned reads displayed.

AGCGTATTGGGAACGATAAACTCTCATGACATATGGCTACAGTGAATATTTTGGGGGGAATTCTCCGAAGCCGGGAAGGATAATTTTTGCACAGAGAAATTACGATATGAAACGCGGGCACAGTTATCACCAGCCCGCC‑G‑AA‑A‑A‑GAGAGAGAATCAGGATGCGGTTAAGATACCGCCAATCATCGCGCGTAATCCCTGAGCAAACGACGCTTCACGCGCCAGGTCTTCCGCTTCGATATCGGCCAGTACACCGTAGGTCAGATCCAGCAGTAAA  >  NZ_CP025268/1701126‑1701399

aGCGTATTGGGAACGATAAACTCTCATGACATATGGATACAGTGAATATTTAGGGGGGAATTCTCCGAAGCCGGGAAGGATAATTTTTTCACAGATAAATTACGATATGAAACGCGGGCACAGTTATCACCAGCccgcc‑g                                                                                                                                            >  2:3846638/1‑140 (MQ=255)

 gCGTATTGGGAACGATAAACTCTCATGACATATGGCTACAGTGAATATTTTGGGGGGAATTCTCCGAAGCCGGGAAGGATAATTTTTGCACAGAGAAATTACGATATGAAACGCGGGCACAGTTATCACCAGCCCGCC‑G‑a                                                                                                                                          <  2:4076312/140‑1 (MQ=255)

  cGTATTGGGAACGATAAACTCTCATGACATATGGCTACAGTGAATATTTTGGGGGGAATTCTCCGAAGCCGGGAAGGATAATTTTTGCACAGAGAAATTACGATATGAAACGCGGGCACAGTTATCACCAGCCCGCC‑G‑aa                                                                                                                                         >  2:6166406/1‑140 (MQ=255)

  cGTATTGGGAACGATAAACTCTCATGACATATGGCTACAGTGAATATTTTGGGGGGAATTCTCCGAAGCCGGGAAGGATAATTTTTGCACAGAGAAATTACGATATGAAACGCGGGCACAGTTATCACCAGCCCGCC‑G‑aa                                                                                                                                         >  1:6423956/1‑140 (MQ=255)

         ggAACGATAAACTCTCATGACATATGGCTACAGTGAATATTTTGGGGGGAATTCTCCGAAGCCGGGAAGGATAATTTTTGCACAGAGAAATTACGATATGAAACGCGGGCACAGTTATCACCAGCCCGCC‑G‑AA‑a‑a‑‑agaga                                                                                                                              <  2:2799730/140‑1 (MQ=255)

           aaCGATAAACTCTCATGACATATGGCTACAGTGAATATTTTTGGGGGAATTCTCCGAAGCCGGGAAGGATAATTTTTGCACAGAGAAATTACGATATGAAACGCGGGCACAGTTATCACCAGCCCGCC‑G‑AA‑A‑a‑‑agagaga                                                                                                                            >  2:7273647/1‑140 (MQ=255)

           aaCGATAAACTCTCATGACATATGGCTACAGTGAATATTTTGGGGGGAATTCTCCGAAGCCGGGAAGGATAATTTTTGCACAGAGAAATTACGATATGAAACGCGGGCACAGTTATCACCAGCCCGCC‑G‑AA‑A‑a‑‑agagaga                                                                                                                            >  1:3456910/1‑140 (MQ=255)

           aaCGATAAACTCTCATGACATATGGCTACAGTGAATATTTTGGGGGGAATTCTCCGAAGCCGGGAAGGATAATTTTTGCACAGAGAAATTACGATATGAAACGCGGGCACAGTTATCACCAGCCCGCC‑G‑AA‑A‑a‑‑agagaga                                                                                                                            <  2:862001/140‑1 (MQ=255)

              gATAAACTCTCATGACATATGGCTACAGTGAATATTTTGGGGGGAATTCTCCGAAGCCGGGAAGGATAATTTTTGCACAGAGAAATTACGATATGAAACGCGGGCACAGTTATCACCAGCCCGCC‑GAAA‑A‑A‑GAGAGAGaa                                                                                                                           >  2:3005394/1‑140 (MQ=255)

              gATAAACTCTCATGACATATGGCTACAGTGAATATTTTGGGGGGAATTCTCCGAAGCCGGGAAGGATAATTTTTGCACAGAGAAATTACGATATGAAACGCGGGCACAGTTATCACCAGCCCGCC‑GAAA‑A‑A‑GAGAGAGaa                                                                                                                           >  2:3291977/1‑140 (MQ=255)

              gATAAACTCTCATGACATATGGCTACAGTGAATATTTTGGGGGGAATTCTCCGAAGCCGGGAAGGATAATTTTTGCACAGAGAAATTACGATATGAAACGCGGGCACAGTTATCACCAGCCCGCC‑GAAA‑A‑A‑GAGAGAGaa                                                                                                                           >  1:7706439/1‑140 (MQ=255)

                   aCTCTCATGACATATGGCTACAGTGAATATTTTGGGGGGAATTCTCCGAAGCCGGGAAGGATAATTTTTGCACAGAGAAATGACGATATGAAACGCGGGCACAGTTATCACCAGCCCGCC‑GAAA‑A‑A‑GAGAGAGAATCAgg                                                                                                                      <  2:7447612/140‑1 (MQ=255)

                     tctcATGACATATGGCTACAGTGAATATTTTGGGGGGAATTCTCCGAAGCCGGGAAGGATAATTTTTGCACAGAGAAATTACGATATGAAACGCGGGCACAGTTATCACCAGCCCGCC‑GAAA‑A‑A‑GAGAGAGAATCAGGAt                                                                                                                    <  1:720864/140‑1 (MQ=255)

                     tctcATGACATATGGCTACAGTGAATATTTTGGGGGGAATTCTCCGAAGCCGGGAAGGATAATTTTTGCACAGAGAAATTACGATATGAAACGCGGGCACAGTTATCACCAGCCCGCC‑GAAA‑A‑A‑GAGAGAGAATCAGGAt                                                                                                                    <  1:1932030/140‑1 (MQ=255)

                     tctcATGACATATGGCTACAGTGAATATTTTGGGGGGAATTCTCCGAAGCCGGGAAGGATAATTTTTGCACAGAGAAATTACGATATGAAACGCGGGCACAGTTATCACCAGCCCGCC‑GAAA‑A‑A‑GAGAGAGAATCAGGAt                                                                                                                    <  1:1639360/140‑1 (MQ=255)

                     tctcATGACATATGGCTACAGTGAATATTTTGGGGGGAATTCTCCGAAGCCGGGAAGGATAATTTTTGCACAGAGAAATTACGATATGAAACGCGGGCACAGTTATCACCAGCCCGCC‑GAAA‑A‑A‑GAGAGAGAATCAGGAt                                                                                                                    <  1:823815/140‑1 (MQ=255)

                     tctcATGACATATGGCTACAGTGAATATTTTGGGGGGAATTCTCCGAAGCCGGGAAGGATAATTTTTGCACAGAGAAATTACGATATGAAACGCGGGCACAGTTATCACCAGCCCGCC‑GAAA‑A‑A‑GAGAGAGAATCAGGAt                                                                                                                    <  2:598771/140‑1 (MQ=255)

                     tctcATGACATATGGCTACAGTGAATATTTTGGGGGGAATTCTCCGAAGCCGGGAAGGATAATTTTTGCACAGAGAAATTACGATATGAAACGCGGGCACAGTTATCACCAGCCCGCC‑GAAA‑A‑A‑GAGAGAGAATCAGGAt                                                                                                                    <  1:1106274/140‑1 (MQ=255)

                      ctcATGACATATGGCTACAGTGAATATTTTGGGGGGAATTCTCCGAAGCCGGGAAGGATAATTTTTGCACAGAGAAATTACGATATGAAACGCGGGCACAGTTATCACCAGCCCGCC‑GAAA‑A‑A‑GAGAGAGAATCAGGATg                                                                                                                   <  2:7138614/140‑1 (MQ=255)

                       tcATGACATATGGCTACAGTGAATATTTTGGGGGGAATTCTCCGAAGCCGGGAAGGATAATTTTTGCACAGAGAAATTACGATATGAAACGCGGGCACAGTTATCACCAGCCCGCC‑GAAA‑A‑A‑GAGAGAGAATCAGGATGc                                                                                                                  <  2:4505978/140‑1 (MQ=255)

                       tcATGACATATGGCTACAGTGAATATTTTGGGGGGAAGTCTCCGAAGCCGGGAAGGATAATTTTTGCACAGAGAAATTACGATATGAAACGCGGGCACAGTTATCACCAGCCCGCC‑GAAA‑A‑A‑GAGAGAGAATCAGGATGc                                                                                                                  <  2:1744263/140‑1 (MQ=255)

                        cATGACATATGGCTACAGTGAATATTTTGGGGGGAATTCTCCGAAGCCGGGAAGGATAATTTTTGCACAGAGAAATTACGATATGAAACGCGGGCACAGTTATCACCAGCCCGCC‑GAAA‑A‑A‑GAGAGAGAATCAGGATGCg                                                                                                                 >  2:3578058/1‑140 (MQ=255)

                          tGACATATGGCTACAGTGAATATTTTGGGGGGTATTCTCCGAAGCCGGGAAGGATAATTTTTGCACAGAGAAATTACGATATGAAACGCGGGCACAGTTATCACCAGCCCGCC‑GAAA‑A‑A‑GAGAGAGAATCAGGATGCGGt                                                                                                               <  2:5468837/140‑1 (MQ=255)

                          tGACATATGGCTACAGTGAATATTTTGGGGGGAATTCTCCGAAGCCGGGAAGGATAATTTTTGCACAGAGAAATTACGATATGAAACGCGGGCACAGTTATCACCAGCCCGCC‑GAAA‑A‑A‑GAGAGAGAATCAGGATGCGGt                                                                                                               >  1:495623/1‑140 (MQ=255)

                          tGACATATGGCGACAGTGAATATTTTGGGGGGAATTCTCCGAAGCCGGGAAGGATAATTTTTGCACAGAGAAATTACGATATGAAACGCGGGCACAGTTATCACCAGCCCGCC‑GAAA‑A‑A‑GAGAGAGAATCAGGATGCGGt                                                                                                               <  1:7879963/140‑1 (MQ=255)

                               tatGGCTACAGTGAATATTTTGGGGGGAATTCTCCGAAGCCGGGAAGGATAATTTTTGCACAGAGAAATTACGATATGAAACGCGGGCACAGTTATCACCAGCCCGCC‑GAAA‑A‑A‑GAGAGAGAATCAGGATGCGGTTAAGa                                                                                                          <  1:2181265/140‑1 (MQ=255)

                               tatGGCTACAGTGAATATTTTGGGGGGAATTCTCCGAAGCCGGGAAGGATAATTTTTGCACAGAGAAATTACGATATGAAACGCGGGCACAGTTATCACCAGCCCGCC‑GAAA‑A‑A‑GAGAGAGAATCAGGATGCGGTTAAGa                                                                                                          <  1:3319396/140‑1 (MQ=255)

                                 tGGCTACAGTGAATATTTTGGGGGGAATTCTCCGAAGCCGGGAAGGATAATTTTTGCACAGAGAAATTACGATATGAAACGCGGGCACAGTTATCACCAGCCCGCC‑GAAA‑A‑A‑GAGAGAGAATCAGGATGCGGTTAAGATa                                                                                                        <  1:8964730/140‑1 (MQ=255)

                                 tGGCTACAGTGAATATTTTGGGGGGAATTCTCCGAAGACGGGAAGGATAATTTTTGCACAGAGAAATTACGATATGAAACGCGGGCACAGTTATCACCAGCCCGCC‑GAAA‑A‑A‑GAGAGAGAATCAGGATGCGGTTAAGATa                                                                                                        >  2:2048189/1‑140 (MQ=255)

                                     tACAGTGAATATTTTGGGGGGAATTCTCCGAAGCCGGGAAGGATAATTTTTGCACAGAGAAATTACGATATGAAACGCGGGCACAGTTATCACCAGCCCGCC‑GAAA‑A‑A‑GAGAGAGAATCAGGATGCGGTTAAGATACCGc                                                                                                    >  1:624267/1‑140 (MQ=255)

                                       cAGTGAATATTTTGGGGGGAATTCTCCGAAGCCGGGAAGGATAATTTTTGCACAGAGAAATTACGATATGAAACGCGGGCACAGTTATCACCAGCCCGCC‑GAAA‑A‑A‑GAGAGAGAATCAGGATGCGGTTAAGATACCGCCa                                                                                                  >  1:5767215/1‑140 (MQ=255)

                                       cAGTGAATATTTTGGGGGGAATTCTCCGAAGCCGGGAAGGATAATTTTTGCACAGAGAAATTACGATATGAAACGCGGGCACAGTTATCACCAGCCCGCC‑GAAA‑A‑A‑GAGAGAGAATCAGGATGCGGTTAAGATACCGCCa                                                                                                  >  1:6088226/1‑140 (MQ=255)

                                       cAGTGAATATGTTGGGGGGAATTCTCCGAAGCCGGGAAGGATAATTTTTGCACAGAGAAATTACGATATGAAACGCGGGCACAGTTATCACCAGCCCGCC‑GAAA‑A‑A‑GAGAGAGAATCAGGATGCGGTTAAGATACCGCCa                                                                                                  >  2:8992673/1‑140 (MQ=255)

                                        aGTGATTATGGTGGGGGGAATTCTCCGAAGCCGGGAAGGATAATTTTTGCACAGAGAACTTACGATATGAATCGCGGGCACAGTTAGCACCAGCCCGCC‑G‑AACA‑G‑GAGAGAGAATCCGGATGCGGTTAAGATACCGCCat                                                                                                 >  2:3108250/1‑139 (MQ=255)

                                        aGTGAATATTTTGGGGGGAATTCTCCGAAGCCGGGAAGGATAATTTTTGCACAGAGAAATTACGATATGAAACGCGGGCACAGTTATCACCAGCCCGCC‑GAAA‑A‑A‑GAGAGAGAATCAGGATGCGGTTAAGATACCGCCaa                                                                                                 >  1:2244574/1‑140 (MQ=255)

                                            aaTATTTTGGGGGGAATTCTCCGAAGCCGGGAAGGATAATTTTTGCACAGAGAAATTACGATATGAAACGCGGGCACAGTTATCACCAGCCCGCC‑GAAA‑A‑A‑GAGAGAGAATCAGGATGCGGTTAAGATACCGCCAatcat                                                                                             >  1:3122142/1‑140 (MQ=255)

                                            aaTATTTTGGGGGGAATTCTCCGAAGCCGGGAAGGATAATTTTTGCACAGAGAAATTACGATATGAAACGCGGGCACAGTTATCACCAGCCCGCC‑GAAA‑A‑A‑GAGAGAGAATCAGGATGCGGTTAAGATACCGCCAatcat                                                                                             >  1:2612863/1‑140 (MQ=255)

                                             atatTTTGGGGGGAATTCTCCGAAGCCGGGAAGGATAATTTTTGCACAGAGAAATTACGATATGAAACGCGGGCACAGTTATCACCAGCCCGCC‑GAAA‑A‑A‑GAGAGAGAATCAGGATGCGGTTAAGATACCGCCAatcatc                                                                                            <  1:1737136/140‑1 (MQ=255)

                                             atatTTTGGGGGGAATTCTCCGAAGCCGGGAAGGATAATTTTAGCACAGAGAAATTACGATATGAAACGCGGGCACAGTTATCACCAGCCCGCCTA‑AA‑A‑A‑GAGAGAGAATCAGGATGCGGTTAAGATACCGCCAatcatc                                                                                            >  1:7499614/1‑140 (MQ=255)

                                              tatTTTGGGGGGAATTCTCCGAAGCCGGGAAGGATAATTTTTGCACAGAGAAATTACGATATGAAACGCGGGCACAGTTATCACCAGCCCGCC‑GAAA‑A‑A‑GAGAGAGAATCAGGATGCGGTTAAGATACCGCCAATCATcg                                                                                           <  1:3816882/140‑1 (MQ=255)

                                              tatTTTGGGGGGAATTCTCCGAAGCCGGGAAGGATAATTTTTGCACAGAGAAATTACGATATGAAACGCGGGCACAGTTATCACCAGCCCGCC‑GAAA‑A‑A‑GAGAGAGAATCAGGATGCGGTTAAGATACCGCCAATCATcg                                                                                           >  1:5568615/1‑140 (MQ=255)

                                              tatTTTGGGGGGAATTCTCCGAAGCCGGGAAGGATAATTTTTGCACAGAGAAATTACGATATGAAACGCGGGCACAGTTATCACCAGCCCGCC‑GAAA‑A‑A‑GAGAGAGAATCAGGATGCGGTTAAGATACCGCCAATCATcg                                                                                           <  1:8653471/140‑1 (MQ=255)

                                              tatTTTGGGGGGAATTCTCCGAAGCCGGGAAGGATAATTTTTGCACAGAGAAATTACGATATGAAACGCGGGCACAGTTATCACCAGCCCGCC‑GAAA‑A‑A‑GAGAGAGAATCAGGATGCGGTTAAGATACCGCCAATCATcg                                                                                           <  2:5040753/140‑1 (MQ=255)

                                                    ggggggAATTCTCCGAAGCCGGGAAGGATAATTTTTGCACAGAGAAATTACGATATGAAACGCGGGCACAGTTATCACCAGCCCGCC‑GAAA‑A‑A‑GAGAGAGAATCAGGATGCGGTTAAGATACCGCCAATCATCGCGCGTa                                                                                     <  2:1174049/140‑1 (MQ=255)

                                                      ggggAATTCTCCGAAGCCGGGAAGGATAATTTTTGCACAGAGAAATTACGATATGAAACGCGGGCACAGTTATCACCAGCCCGCC‑GAAA‑A‑A‑GAGAGAGAATCAGGATGCGGTTAAGATACCGCCAATCATCGCGCGTAAt                                                                                   >  2:5677308/1‑140 (MQ=255)

                                                        ggAATTCTCCGAAGCCGGGAAGGATAATTTTTGCACAGAGAAATTACGATATGAAACGCGGGCACAGTTATCACCAGCCCGCC‑GAAA‑A‑A‑GAGAGAGAATCAGGATGCGGTTAAGATACCGCCAATCATCGCGCGTAATcc                                                                                 >  2:4416476/1‑140 (MQ=255)

                                                          aaTTCTCCGAAGCCGGGAAGGATAATTTTTGCACAGAGACATTACGATATGAAACGCGGGCACAGATATCACCAGCCCGCC‑GAAA‑A‑A‑GAGAGAGAATCAGGATGCGGTTAAGATACCGCCAATCATCGCGCGTAATCCCt                                                                               >  1:4709039/1‑140 (MQ=255)

                                                          aaTTCTCCGAAGCCGGGAAGGATAATTTTTGCACAGAGAAATTACGATATGAAACGCGGGCACAGTTATCACCAGCCCGCC‑GAAA‑A‑A‑GAGAGAGAATCAGGATGCGGTTAAGATACCGCCAATCATCGCGCGTAATCCCt                                                                               >  1:9371325/1‑140 (MQ=255)

                                                          aaTTCTCCGAAGCCGGGAAGGATAATTTTTGCACAGAGAAATTACGATATGAAACGCGGGCACAGTTATCACCAGCCCGCC‑GAAA‑A‑A‑GAGAGAGAATCAGGATGCGGTTAAGATACCGCCAATCATCGCGCGTAATCCCt                                                                               >  2:132591/1‑140 (MQ=255)

                                                                  gAAGCCGGGAAGGATAATTTTTGCACAGAGAAATTACGATATGAAACGCGGGCACAGTTATCACCAGCCCGCC‑GAAA‑A‑A‑GAGAGAGAATCAGGATGCGGTTAAGATACCGCCAATCATCGCGCGTAATCCCTGAGCAAac                                                                       <  1:4261931/140‑1 (MQ=255)

                                                                    aGCCGGGAAGGATAATTTTTGCACAGAGAAATTACGATATGAAACGCGGGCACAGTTATCACCAGCCCGCC‑GAAA‑A‑A‑GAGAGAGAATCAGGATGCGGTTAAGATACCGCCAATCATCGCGCGTAATCCCTGAGCAAacga                                                                     >  1:600085/1‑140 (MQ=255)

                                                                         ggAAGGATAATTTTTGCACAGAGAAATTACGATATGAAACGCGGGCACAGTTATCACCAGCCCGCC‑GAAA‑A‑A‑GAGAGAGAATCAGGATGCGGTTAAGATACCGCCAATCATCGCGCGTAATCCCTGAGCAAACGACGCtt                                                                >  2:9291506/1‑140 (MQ=255)

                                                                         ggAAGGATAATTTTTGCACAGAGAAATTACGATATGAAACGCGGGCACAGTTATCACCAGCCCGCC‑GAAA‑A‑A‑GAGAGAGAATCAGGATGCGGTTAAGATACCGCCAATCATCGCGCGTAATCCCTGAGCAAACGACGCtt                                                                >  1:997292/1‑140 (MQ=255)

                                                                         ggAAGGATAATTTTTGCACAGAGAAATTACGATATGAAACGCGGGCACAGTTATCACCAGCCCGCC‑GAAA‑A‑A‑GAGAGAGAATCAGGATGCGGTTAAGATACCGCCAATCATCGCGCGTAATCCCTGAGCAAACGACGCtt                                                                >  1:1391528/1‑140 (MQ=255)

                                                                         ggAAGGATAATTTTTGCACAGAGAAATTACGATATGAAACGCGGGCACAGTTATCACCAGCCCGCC‑GAAA‑A‑A‑GAGAGAGAATCAGGATGCGGTTAAGATACCGCCAATCATCGCGCGTAATCCCTGAGCAAACGACGCtt                                                                >  2:6328183/1‑140 (MQ=255)

                                                                           aaGGATAATTTTTGCACAGAGAAATTACGATATGAAACGCGGGCACAGTTATCACCAGCCCGCC‑GAAA‑A‑A‑GAGAGAGAATCAGGATGCGGTTAAGATACCGCCAATCATCGCGCGTAATCCCTGAGCAAACGACGCTTCa                                                              <  2:495623/140‑1 (MQ=255)

                                                                           aaGGATAATTTTTGCACAGAGAAATTACGATATGAAACGCGGGCACAGTTATCACCAGCCCGCC‑GAAA‑A‑A‑GAGAGAGAATCAGGATGCGGTTAAGATACCGCCAATCATCGCGCGTAATCCCTGAGCAAACGACGCTTCa                                                              <  2:159005/140‑1 (MQ=255)

                                                                            aGGATAATTTTTGCACAGAGAAATTACGATATGAAACGCGGGCACAGTTATCACCAGCCCGCC‑GAAA‑A‑A‑GAGAGAGAATCAGGATGCGGTTAAGATACCGCCAATCATCGCGCGTAATCCCTGAGCAAACGACGCTTCAc                                                             <  2:5258227/140‑1 (MQ=255)

                                                                              gATAATTTTTGCACAGAGAAATTACGATATGAAACGCGGGCACAGTTATCACCAGCCCGCC‑GAAA‑A‑A‑GAGAGAGAATCAGGATGCGGTTAAGATACCGCCAATCATCGCGCGTAATCCCTGAGCAAACGACGCTTCAcgc                                                           <  2:5178377/140‑1 (MQ=255)

                                                                              gATAATTTTTGCACAGAGAAATTACGATATGAAACGCGGGCACAGTTATCACCAGCCCGCC‑GAAA‑A‑A‑GAGAGAGAATCAGGATGCGGTTAAGATACCGCCAATCATCGCGCGTAATCCCTGAGCAAACGACGCTTCAcgc                                                           >  1:924484/1‑140 (MQ=255)

                                                                                         cacaGAGAAATTACGATATGAAACGCGGGCACAGTTATCACCAGCCCGCC‑GAAA‑A‑A‑GAGAGAGAATCAGGATGCGGTTAAGATACCGCCAATCATCGCGCGTAATCCCTGAGCAAACGACGCTTCACGCGCCAGGTCTTc                                                <  2:4699919/140‑1 (MQ=255)

                                                                                         cacaGAGAAATTACGATATGAAACGCGGGCACAGTTATCACCAGCCCGCC‑GAAA‑A‑A‑GAGAGAGAAGCAGGATGCGGTTAAGATACCGCCAATCATCGCGCGTAATCCCTGAGCAAACGACGCTTCACGCGCCAGGTCTTc                                                <  1:8175126/140‑1 (MQ=255)

                                                                                         cacaGAGAAATTACGATATGAAACGCGGGCACAGTTATCACCAGCCCGCC‑G‑AA‑A‑ACGAGAGAGAATCAGGATGCGGTTAAGATACCGCCAATCATCGCGCGTAATCCCTGAGCAAACGACGCTTCACGCGCCAGGTCTTc                                                <  2:2263884/140‑1 (MQ=255)

                                                                                              agaAATTACGATATGAAACGCGGGCACAGTTATCACCAGCCCGCC‑G‑AA‑ATA‑GAGAGAGAATCAGGATGCGGTTAAGATACCGCCAATCATCGCGCGTAATCCCTGAGCAAACGACGCTTCACGCGCCAGGTCTTCCGCtt                                           >  2:5838417/1‑140 (MQ=255)

                                                                                                   ttACGTTATGAAACGCGGGCACAGTTATCACCAGCCCGCC‑GAAA‑A‑A‑GAGAGAGAATCAGGATGCGGTTAAGATACCGCCAATCATCGCGCGTAATCCCTGAGCAAACGACGCTTCACGCGCCAGGTCTTCCGCTTCGata                                      <  1:398482/140‑1 (MQ=255)

                                                                                                   ttACGATATGAAACGCGGGCACCGGTATCACCAGCCCGCC‑GAAA‑A‑A‑GCGATAGCATCAGGTTGCGGTTAAGATACCGCCAATCTTCGCGCGTAATCCCGGAGCAAACGACGCTTCACGCGCCAGGTCTTCCGCGTCGata                                      <  2:5872686/140‑1 (MQ=255)

                                                                                                   ttACGATATGAAACGCGGGCACAGTTATCACCAGCCCGCC‑GAAA‑A‑A‑GAGAGAGAATCAGGATGCGGTTAAGATACCGCCAATCATCGCGCGTAATCCCTGAGCAAACGACGCTTCACGCGCCAGGTCTTCCGCTTCGata                                      <  2:8079390/140‑1 (MQ=255)

                                                                                                   ttACGATATAAAACGCGGGCACAGTTATCACCAGCCCGCC‑GAAA‑A‑A‑GAGAGAGAATCAGGATGCGGTTAAGATACCGCCAATCATCGCGCGTAATCCCTGAGCAAACGACGCTTCACGCGCCAGGTCTTCCGCTTCGata                                      <  2:6751516/140‑1 (MQ=255)

                                                                                                     aCGATATGAACCGCGGGCACAGTTATCACCAGCCCGCC‑GAAA‑A‑A‑GAGAGAGACTCAGGATGCGGTTAAGATACCGCCAATCATCGCGCGTAATCCCTGCGCAAACGACGCTTCACGCGCCAGGTCTTCCGCTTCGATATc                                    <  2:3442634/140‑1 (MQ=255)

                                                                                                     aCGATATGAAACGCGGGCACAGTTATCACCAGCCCGCC‑GAAA‑A‑A‑GAGAGAGAATCAGGATGCGGTTAAGATACCGCCAATCATCGCGCGTAATCCCTGAGCAAACGACGCTTCACGCGCCAGGTCTTCCGCTTCGATATc                                    <  1:2932531/140‑1 (MQ=255)

                                                                                                     aCGATATGAAACGCGGGCACAGTTATCACCAGCCCGCC‑GAAA‑A‑A‑GAGAGAGAATCAGGATGCGGTTAAGATACCGCCAATCATCGCGCGTAATCCCTGAGCAAACGACGCTTCACGCGCCAGGTCTTCCGCTTCGATATc                                    <  2:880888/140‑1 (MQ=255)

                                                                                                     aCGATATGAAACGCGGGCACAGTTATCACCAGCCCGCC‑GAAA‑A‑A‑GAGAGAGAATCAGGATGCGGTTAAGATACCGCCAATCATCGCGCGTAATCCCTGAGCAAACGACGCTTCACGCGCCAGGTCTTCCGCTTCGATATc                                    <  2:242910/140‑1 (MQ=255)

                                                                                                        atatGAAACGCGGGCACAGTTATCACCAGCCCGCCTA‑AA‑A‑A‑GAGAGAGAATCAGTATGCGGTTAATATACCGCAAATCATCGCGCGTAATCCCTGAGAAAACGACGCTTCACGCGCCAGGTCTTCCGCTTCGATATCGGc                                 <  1:6647498/140‑1 (MQ=255)

                                                                                                        atatGAAACGCGGGCACAGTTATCACCAGCCCGCC‑GAAA‑A‑A‑GAGAGAGAATCAGGATGCGGTTAAGATACCGCCAATCATCGCGCGTAATCCCTGAGCAAACGACGCTTCACGCGCCAGGTCTTCCGCTTCGATATCGGc                                 <  2:1283297/140‑1 (MQ=255)

                                                                                                        atatGAAACGCGGGCACAGTTATCACCAGCCCGCC‑GAAA‑A‑A‑GAGAGAGAATCAGGATGCGGTTAAGATACCGCCAATCATCGCGCGTAATCCCTGAGCAAACGACGCTTCACGCGCCAGGTCTTCCGCTTCGATATCGGc                                 <  1:8502558/140‑1 (MQ=255)

                                                                                                        atatGAAACGCGGGCACAGTTATCACCAGCCCGCC‑GAAA‑A‑A‑GAGAGAGAATCAGGATGCGGTTAAGATACCGCCAATCATCGCGCGTAATCCCTGAGCAAACGACGCTTCACGCGCCAGGTCTTCCGCTTCGATATCGGc                                 >  2:6889587/1‑140 (MQ=255)

                                                                                                        atatGAAACGCGGGCACAGTTATCACCAGCCCGCC‑GAAA‑A‑A‑GAGAGAGAATCAGGATGCGGTTAAGATACCGCCAATCATCGCGCGTAATCCCTGAGCAAACGACGCTTCACGCGCCAGGTCTTCCGCTTCGATATCGGc                                 <  1:5226166/140‑1 (MQ=255)

                                                                                                        atatGAAACGCGGGCACAGTTATCACCAGCCCGCC‑GAAA‑A‑A‑GAGAGAGAATCAGGATGCGGTTAAGATACCGCCAATCATCGCGCGTAATCCCTGAGCAAACGACGCTTCACGCGCCAGGTCTTCCGCTTCGATATCGGc                                 >  2:7542347/1‑140 (MQ=255)

                                                                                                        atatGAAACGCGGGCACAGTTATCACCAGCCCGCC‑GAAA‑A‑A‑GAGAGAGAATCAGGATGCGGTTAAGATACCGCCAATCATCGCGCGTAATCCCTGAGCAAACGACGCTTCACGCGCCAGGTCTTCCGCTTCGATATCGGc                                 >  1:5853198/1‑140 (MQ=255)

                                                                                                        atatGAAACGCGGGCACAGTTATCACCAGCCCGCC‑GAAA‑A‑A‑GAGAGAGAATCAGGATGCGGTTAAGATACCGCCAATCATCGCGCGTAATCCCTGAGCAAACGACGCTTCACGCGCCAGGTCTTCCGCTTCGATATCGGc                                 <  2:3732803/140‑1 (MQ=255)

                                                                                                        atatGAAACGCGGGCACAGTTATCACCAGCCCGCC‑GAAA‑A‑A‑GAGAGAGAATCAGGATGCGGTTAAGATACCGCCAATCATCGCGCGTAATCCCTGAGCAAACGACGCTTCACGCGCCAGGTCTTCCGCTTCGATATCGGc                                 <  2:8129657/140‑1 (MQ=255)

                                                                                                        atatGAAACGCGGGCACAGTTATCACCAGCCCGCC‑GAAA‑A‑A‑GAGAGAGAATCAGGATGCGGTTAAGATACCGCCAATCATCGCGCGTAATCCCTGAGCAAACGACGCTTCACGCGCCAGGTCTTCCGCTTCGATATCGGc                                 >  2:1808338/1‑140 (MQ=255)

                                                                                                             aaaCGCGGGCACAGTTATCACCAGCCCGCC‑GAAA‑A‑A‑GAGAGAGAATCAGGATGCGGTTAAGATACCGCCAATCATCGCGCGTAATCCCTGAGCAAACGACGCTTCACGCGCCAGGTCTTCCGCTTCGATATCGGCCAGTa                            <  1:201184/140‑1 (MQ=255)

                                                                                                                cgcgGGCACAGTTATCACCAGCCCGCC‑GAAA‑A‑A‑GAGAGAGAATCAGGATGCGGTTAAGATACCGCCAATCATCGCGCGTAATCCCTGAGCAAACGACGCTTCACGCGCCAGGTCTTCCGCTTCGATATCGGCCAGTacac                         >  1:8876286/1‑140 (MQ=255)

                                                                                                                  cgGGCACAGTTATCACCAGCCCGCC‑GAAA‑A‑A‑GAGAGAGAATCAGGATGCGGTTAAGATACCGCCAATCATCGCGCGTAATCCCTGAGCAAACGACGCTTCACGCGCCAGGTCTTCCGCTTCGATATCGGCCAGTACACCg                       <  1:2717477/140‑1 (MQ=255)

                                                                                                                   gggCACAGTTATCACCAGCCCGCC‑GAAA‑A‑A‑GAGAGAGAATCAGGATGCGGTTAAGATACCGCCAATCATCGCGCGTAATCCCTGAGCAAACGACGCTTCACGCGCCAGGTCTTCCGCTTCGATATCGGCCAGTACACCGt                      >  1:2418417/1‑140 (MQ=255)

                                                                                                                   gggCACAGTTATCACCAGCCCGCC‑GAAA‑A‑A‑GAGAGAGAATCAGGATGCGGTTAAGATACCGCCAATCATCGCGCGTAATCCCTGAGCAAACGACGCTTCACGCGCCAGGTCTTCCGCTTCGATATCGGCCAGTACACCGt                      >  1:4366797/1‑140 (MQ=255)

                                                                                                                   gggCACAGTTATCACCAGCCCGCC‑GAAA‑A‑A‑GAGAGAGAATCAGGATGCGGTTAAGATACCGCCAATCATCGCGCGTAATCCCTGAGCAAACGACGCTTCACGCGCCAGGTCTTCCGCTTCGATATCGGCCAGTACACCGt                      >  1:3668319/1‑140 (MQ=255)

                                                                                                                             aTCACCAGCCCGCC‑GAAA‑A‑A‑GAGAGAGAATCAGGATGCGGTTAAGATACCGCCAATCATCGCGCGTAATCCCTGAGCAAACGACGCTTCACGCGCCAGGTCTTCCGCTTCGATATCGGCCAGTACACCGTAGGTCAGATc            <  1:5116620/140‑1 (MQ=255)

                                                                                                                              tCACCAGCCCGCC‑GAAA‑A‑A‑GAGAGAGAATCAGGATGCGGTTAAGATACCGCCAATCATCGCGCGTAATCCCTGAGCAAACGACGCTTCACGCGCCAGGTCTTCCGCTTCGATATCGGCCAGTACACCGTAGGTCAGATcc           <  1:8362211/140‑1 (MQ=255)

                                                                                                                              tCACCAGCCCGCC‑GAAA‑A‑A‑GAGAGAGAATCAGGATGCGGTTAAGATACCGCCAATCATCGCGCGTAATCCCTGAGCAAACGACGCTTCACGCGCCAGGTCTTCCGCTTCGATATCGGCCAGTACACCGTAGGTCAGATcc           <  1:6765667/140‑1 (MQ=255)

                                                                                                                                   aGCCCGCC‑GAAA‑A‑A‑GAGAGAGAATCAGGATGCGGTTAAGATACCGCCAATCATCGCGCGTAATCCCTGAGCAAACGACGCTTCACGCGCCAGGTCTTCCGCTTCGATATCGGCCAGTACACCGTAGGTCAGATCcagcag      >  1:6570667/1‑140 (MQ=255)

                                                                                                                                   aGCCCGCC‑GAAA‑A‑A‑GAGAGAGAATCAGGATGCGGTTAAGATACCGCCAATCATCGCGCGTAATCCCTGAGCAAACGACGCTTCACGCGCCAGGTCTTCCGCTTCGATATCGGCCAGTACACCGTAGGTCAGATCcagcag      >  1:7117796/1‑140 (MQ=255)

                                                                                                                                   aGCCCGCC‑GAAA‑A‑A‑GAGAGAGAATCAGGATGCGGTTAAGATACCGCCAATCATCGCGCGTAATCCCTGAGCAAACGACGCTTCACGCGCCAGGTCTTCCGCTTCGATATCGGCCAGTACACCGTAGGTCAGATCcagcag      <  1:1031602/140‑1 (MQ=255)

                                                                                                                                     cccGCC‑GAAA‑A‑A‑GAGAGAGAATCAGGATGCGGTTAAGATACCGCCAATCATCGCGCGTAATCCCTGAGCAAACGACGCTTCACGCGCCAGGTCTTcc                                               >  2:2649975/1‑97 (MQ=255)

                                                                                                                                     cccGCC‑GAAA‑A‑A‑GAGAGAGAATCAGGATGCGGTTAAGATACCGCCAATCATCGCGCGTAATCCCTGAGCAAACGACGCTTCACGCGCCAGGTCTTCCGCTTCGATGTCGGCCAGTACACCGTAGGTCAGATCCAGCAGTa    <  2:827787/140‑1 (MQ=255)

                                                                                                                                     cccGCC‑GAAA‑A‑A‑GAGAGAGAATCAGGATGCGGTTAAGATACCGCCAATCATCGCGCGTAATCCCTGAGCAAACGACGCTTCACGCGCCAGGTCTTCCGCTTCGATATCGGCCAGTACACCGTAGGTCAGATCCAGCAGTa    <  2:6543694/140‑1 (MQ=255)

                                                                                                                                     cccGCC‑GAAA‑A‑A‑GAGAGAGAATCAGGATGCGGTTAAGATACCGCCAATCATCGCGCGTAATCCCTGAGCAAACGACGCTTCACGCGCCAGGTCTTCCGCTTCGATATCGGCCAGTACACCGTAGGTCAGATCCAGCAGTa    >  1:4606317/1‑140 (MQ=255)

                                                                                                                                     cccGCC‑GAAA‑A‑A‑GAGAGAGAATCAGGATGCGGTTAAGATACCGCCAATCATCGCGCGTAATCCCTGAGCAAACGACGCTTCACGCGCCAGGTCTTCCGCTTCGATATCGGCCAGTACACCGTAGGTCAGATCCAGCAGTa    >  2:7807091/1‑140 (MQ=255)

                                                                                                                                     cccGCC‑GAAA‑A‑A‑GAGAGAGAATCAGGATGCGGTTAAGATACCGCCAATCATCGCGCGTAATCCCTGAGCAAACGACGCTTCACGCGCCAGGTCTTCCGCTTCGATATCGGCCAGTACACCGTAGGTCAGATCCAGCAGTa    >  1:5471532/1‑140 (MQ=255)

AGCGTATTGGGAACGATAAACTCTCATGACATATGGCTACAGTGAATATTTTGGGGGGAATTCTCCGAAGCCGGGAAGGATAATTTTTGCACAGAGAAATTACGATATGAAACGCGGGCACAGTTATCACCAGCCCGCC‑G‑AA‑A‑A‑GAGAGAGAATCAGGATGCGGTTAAGATACCGCCAATCATCGCGCGTAATCCCTGAGCAAACGACGCTTCACGCGCCAGGTCTTCCGCTTCGATATCGGCCAGTACACCGTAGGTCAGATCCAGCAGTAAA  >  NZ_CP025268/1701126‑1701399

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`