BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP025268	1,855,625	+C	intergenic (+116/‑50)	sppA → / → ansA	signal peptide peptidase SppA/asparaginase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP025268	1,855,621	1	.	C	98.7%	1786.1 / 12.7	520	intergenic (+112/‑54)	sppA/ansA	signal peptide peptidase SppA/asparaginase
Reads supporting (aligned to +/- strand): ref base . (2/5); new base C (220/293); total (222/298)
Fisher's exact test for biased strand distribution p-value = 7.04e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 542 total aligned reads displayed.

TTGCCTGACCTGCGCCAACATGCGTTAAGTCTTGTACTGAGTGGCCGACAGATCGTCGGCCACATTATTTTTTACGTCGACGAATCCTCTTCCCGCTGTTTCGCCCCATATTTCCTTATCCGCATAGTATCAGGTGCGC‑T‑C‑CCCTGCCTCACGTATATACTTTTGCTCTTTCGATATCATTCATATCAATATCATGCAAAAGAAATCAATTTACGTTGCCTACACGGGCGGGACCATCGGGATGCAGCGTTCCGAGCAGGGTTATATACCGGTG  >  NZ_CP025268/1855482‑1855755

ttGCCTGACCTGCGCCAACATGCGTTAAGTCTTGTACTGAGTGGCCGACAGATCGTCGGCCACATTATTTTTTACGTCGACGAATCCTCTTCCCGCTGTTTCGCCCCATATTTCCTTATCCGCATAGTATCAGGTGCGC‑t                                                                                                                                          <  2:3694838/140‑1 (MQ=255)

 tGCCTGACCTGCGCCAACATGCGTTAAGTCTTGTACTGAGTGGCCGACAGATCGTCGGCCACATTATTTTTTACGTCGACGAATCCTCTTCCCGCTGTTTCGCCCCATATTTCCTTATCCGCATAGTATCAGGTGCGC‑T‑c                                                                                                                                        >  1:8198495/1‑140 (MQ=255)

 tGCCTGACCTGCGCCAACATGCGTTAAGTCTTGTACTGAGTGGCCGACAGATCGTCGGCCACATTATTTTTTACGTCGACGAATCCTCTTCCCGCTGTTTCGCCCCATATTTCCTTATCCGCATAGTATCAGGTGCGC‑T‑c                                                                                                                                        >  1:2058889/1‑140 (MQ=255)

   ccTGACCTGCGCCAACATGCGTTAAGTCTTGTACTGAGTGGCCGACAGATCGTCGGCCACATTATTTTTTACGTCGACGAATCCTCTTCCCGCTGTTTCGCCCCATATTTCCTTATCCGCATAGTATCAGGTGCGC‑T‑c‑cc                                                                                                                                     <  2:3864172/140‑1 (MQ=255)

    cTGACCTGCGCCAACATGCGTTAAGTCTTGTACTGAGTGGCCGACAGATCGTCGGCCACATTATTTTTTACGTCGACGAATCCTCTTCCCGCTGTTTCGCCCCATATTTCCTTATCCGCATAGTATCAGGTGCGC‑T‑c‑ccc                                                                                                                                    <  1:409584/140‑1 (MQ=255)

    cTGACCTGCGCCAACATGCGTTAAGTCTTGTACTGAGTGGCCGACAGATCGTCGGCCACATTATTTTTTACGTCGACGAATCCTCTTCCCGCTGTTTCGCCCCATATTTCCTTATCCGCATAGTATCAGGTGCGC‑T‑c‑ccc                                                                                                                                    <  2:7904909/140‑1 (MQ=255)

          tGCGCCAACATGCGTTAAGTCTTGTACTGAGTGGCCGACAGATCGTCGGCCACATTATTTTTTACGTCGACGAATCCTCTTCCCGCTGTTTCGCCCCATATTTCCTTATCCGCATAGTATCAGGTGCGC‑TCC‑CCCTGCCt                                                                                                                               <  1:2645138/140‑1 (MQ=255)

          tGCGCCAACATGCGTTAAGTCTTGTACTGAGTGGCCGACAGATCGTCGGCCACATTATTTTTTACGTCGACGAATCCTCTTCCCGCTGTTTCGCCCCATATTTCCTTATCCGCATAGTATCAGGTGCGC‑TCC‑CCCTGCCt                                                                                                                               <  2:4952876/140‑1 (MQ=255)

          tGCGCCAACATGCGTTAAGTCTTGTACTGAGTGGCCGACAGATCGTCGGCCACATTATTTTTTACGTCGACGAATCCTCTTCCCGCTGTTTCGCCCCATATTTCCTTATCCGCATAGTATCAGGTGCGC‑TCC‑CCCTGCCt                                                                                                                               <  2:5193394/140‑1 (MQ=255)

          tGCGCCAACATGCGTTAAGTCTTGTACTGAGTGGCCGACAGATCGTCGGCCACATTATTTTTTACGTCGACGAATCCTCTTCCCGCTGTTTCGCCCCATATTTCCTCATCCGCATAGTATCAGGTGCGC‑TCC‑CCCTGCCt                                                                                                                               <  1:1431447/140‑1 (MQ=255)

          tGCGCATACATGCGTTAAGTCTTGTACTGAGTGGCCTACAGATCGTCGGCCACATTATTTTTTACGTCGACGAATCCTCTTCCCGCTGTTTCGCCCCATATTTCCTTATCCGCATAGTATCAGGTGCGC‑T‑c‑cccctgcat                                                                                                                              <  1:3736520/140‑7 (MQ=255)

          agcgcCAACATTCGTTAAGTCTTGTACTGAGTGGCCGACAGATCGTCGGCCACATTATATTTTACGTCGACGAATCCTCTTCCCGCTGTTTCGCCCCATATTTCCTTATCCGCATAGTATCAGGTGCGC‑TCC‑CCCTGCCt                                                                                                                               <  2:6993523/139‑1 (MQ=255)

           gcgcCAACATGCGTTAAGTCTTGTACTGAGTGGCCGACAGATCGTCGGCCACATTATTTTTTACGTCGACGAATCCTCTTCCCGCTGTTTCGCCCCATATTTCCTTATCCGCATAGTATCAGGTGCGC‑TCC‑CCCTGCCTc                                                                                                                              <  2:2322743/140‑1 (MQ=255)

             gcCAACATGCGTTAAGTCTTGTACTGAGTGGCCGACAGATCGTCGGCCACATTATTTTTTACGTCGACGAATCCTCTTCCCGCTGTTTCGCCCCATATTTCCTTATCCGCATAGTATCAGGTGCGC‑TCC‑CCCTGCCTCAc                                                                                                                            >  1:3176059/1‑140 (MQ=255)

                aaCATGCGTTAAGTCTTGTACTGAGTGGCCGACAGATCGTCGGCCACATTATTTTTTACGTCGACGAATCCTCTTCCCGCGGTTTCGCCCCATATTTCCTTATCCGCATAGTATCAGGTGCGC‑TCC‑CCCTGCCTCACGta                                                                                                                         <  2:5372547/140‑1 (MQ=255)

                 aCATGCGTTAAGTCTTGTACTGAGTGGCCGACAGATCGGCGGCCACATTATTTTTTACGTCGACGAATCCTCTTCCCGCTGTTTCGCCCCATATTTCCTTATCCGCATAGGATCAGGTGCGC‑TCC‑CCCTGCCTCACGtat                                                                                                                        >  1:8841935/1‑140 (MQ=255)

                  cATGCGTTAAGTCTTGTACTGAGTGGCCGACAGATCGTCGGCCACATTATTTTTTACGTCGACGAATCCTCTTCCCGCTGTTTCGCCCCATATTTCCTTATCCGCATAGTATCAGGTGCGC‑TCC‑CCCTGCCTCACGtata                                                                                                                       >  2:6695209/1‑140 (MQ=255)

                  cATGCGTTAAGTCTTGTACTGAGTGGCCGACAGATCGTCGGCCACATTATTTTTTACGTCGACGAATCCTCGTCCCGCTGTTTCGCCCCATATTTCCTTATCCGCATAGTATCAGGTGCGC‑TCC‑CCCTGCCTCACGtata                                                                                                                       <  2:7366459/140‑1 (MQ=255)

                      cGTTAAGTCTTGTACTGAGTGGCCGACAGATCGTCGGCCACATTATTTTTTACGTCGACGAATCCTCTTCCCGCTGTTTCGCCCCATATTTCCTTATCCGCATAGTATCAGGTGCGC‑TCC‑CCCTGCCTCACGTATATACt                                                                                                                   >  2:1637672/1‑140 (MQ=255)

                      cGTTAAGTCTTGTACTGAGTGGCCGACAGATCGTCGGCCACATTATTTTTTACGTCGACGAATCCTCTTCCCGCTGTTTCGCCCCATATTTCCTTATCCGCATAGTATCAGGTGCGC‑TCC‑CCCTGCCTCACGTATATACt                                                                                                                   >  1:1127901/1‑140 (MQ=255)

                            gTCTTGTACTGAGTGGCCGACAGATCGTCGGCCACATTATTTTTTACGTCGACGAATCCTCTTCCCGCTGTTTCGCCCCATATTTCCTTATCCGCATAGTATCAGGTGCGC‑TCC‑CCCTGCCTCACGTATATACTTTTGct                                                                                                             <  1:5983070/140‑1 (MQ=255)

                            gTCTTGTACTGAGTGGCCGACAGATCGTCGGCCACATTATTTTGTACGTCGACGAATCCTCTTCCCGCTGTTTCGCCCCATATTTCCTTATCCGGATAGTATCAGGTGCGC‑TCC‑CCCTGCCTCACGTATATACTTTTGct                                                                                                             <  2:9265233/140‑1 (MQ=255)

                                tGTACTGAGTGGCCGACAGATCGTCGGCCACATTATTTTTTACGTCGACGAATCCTCTTCCCGCTGTTTCGCCCCATATTTCCTTATCCGCATAGTATCAGGTGCGC‑TCC‑CCCTGCCTCACGTATATACTTTTGCTCttt                                                                                                         <  1:448933/140‑1 (MQ=255)

                                    cTGAGTGGCCGACAGATCGTCGGCCACATTATTTTTTACGTCGTCGAATCCTCTTCCCGCTGTTTCTCCCCATATTTCCTTATCCGCATAGTATCAGGTGCGC‑T‑CACCCTGCCTCACGTATATACTTTTGCTCTTTCGat                                                                                                     <  1:1923130/140‑1 (MQ=255)

                                    cTGAGTGGCCGACAGATCGTCGGCCACATTATTTTTTACGTCGACGAATCCTCTTCCCGCTGTTTCGCCCCATATTTCCTTATCCGCATAGTATCAGGTGCGC‑TCC‑CCCTGCCTCACGTATATACTTTTGCTCTTTCGat                                                                                                     <  1:1005235/140‑1 (MQ=255)

                                    cTGAGTGGCCGACAGATCGTCGGCCACATTATTTTTTACGTCGACGAATCCTCTTCCCGCTGTTTCGCCCCATATTTCCTTATCCGCATAGTATCAGGTGCGC‑TCC‑CCCTGCCTCACGTATATACTTTTGCTCTTTCGat                                                                                                     <  2:5886708/140‑1 (MQ=255)

                                       aGTGGCCGACAGATCTTCGGACACATTATTTTTTACGTCGACGAATCCTCATCCAGCAGTTTAGCACCATAATTCCATATCCCCATAGTATACGGTGCTC‑TCC‑CCCATCCAAACGTATATAATTTTTCTCTTTAGataac                                                                                                  >  2:7778609/1‑138 (MQ=255)

                                       aGTGGCCGACAGATCGTCGGCCACATTATTTTTTACGTCGACGAATCCTCTTCCCGCTGTTTCGCCCCATATTTCCTTATCCGCATAGTATCTGGTGCGC‑TCC‑CCCTGCCTCACGTATATACTTTTGCTCTTTCGATAtc                                                                                                  >  1:2972119/1‑140 (MQ=255)

                                       aGTGGCCGACAGATCGTCGGCCACATTATTTTTTACGTCGACGAATCCTCTTCCCGCTGTTTCGCCCCATATTTCCTTATCCGCATAGTATCAGGTGCGC‑TCC‑CCCTGCCTCACGta                                                                                                                         >  1:2514772/1‑117 (MQ=255)

                                        gTGGCCGACAGATCGTCGGACACATTAATTTTTACGTCGACGAATCCTCTTCCCGCTGTTTCGCCCCATATTTCCTTATCCGCATAGTATCAGGTGCGC‑TCC‑CCCTGCCTCACGTATATACTTTTGCTCTTTCGATAtca                                                                                                 <  1:5223857/140‑1 (MQ=255)

                                         tGGCCGACAGATCGTCGGCCACATTATTTTTTACGTCGACGAATCCTCTTCCCGCTGTTTCGCCCCATATTTCCTTATCCGCATAGTATCAGGTGCGC‑TCC‑CCCTGCCTCACGTATATACTTTTGCTCTTTCGATAtcat                                                                                                >  2:2749454/1‑140 (MQ=255)

                                         tGGCCGACAGATCGTCGGCCACATTATTTTTTACGTCGACGAATCCTCTTCCCGCTGTTTCGCCCCATATTTCCTTATCCGCATAGTATCAGGTGCGC‑TCC‑CCCTGCCTCACGTATATACTTTTGCTCTTTCGATAtcat                                                                                                >  1:6909817/1‑140 (MQ=255)

                                         gggCCGACAGATCGTCGGCCACATTATTGTTTACGTCGACGAATCCTCTTCCCGCTGTTTCGCCCCATATTTCCTTATCCGCATAGTATCCGGTGCGCGC‑C‑CCCTGCCTCACGTATCTACTTTGGCTCTTTCGATAgcat                                                                                                >  2:6394344/2‑140 (MQ=255)

                                          ggCCGACAGATCGTCTGCCACATTATTTTTTACGTCGACGTATCCTCTTCCCGCTGTTTCGCCCCATATTTCCTTATCCGCATAGTATCTGTTGCGC‑TCC‑CCCTGCCTCACGTATTTATTTTTGCTATTTCGATTtgatt                                                                                               <  2:5576786/140‑1 (MQ=255)

                                          ggCCGACAGATCGTCGGCCACATTATTTTTTACGTCGACGAATCCTCTTCCCGCTGTTTCGCCCCATATTTCCTTATCCGCATAGTATCAGGTGCGC‑TCC‑CCCTGCCTCACGTATATACTTTTGCTCTTTCGATAtcatt                                                                                               <  1:5396771/140‑1 (MQ=255)

                                          ggCCGACAGATCGTCGGCCACATTATTTTTTACGTCGACGAATCCTCTTCCCGCTGTTTCGCCCCATATTTCCTTATCCGCATAGTATCAGGTGCGC‑TCC‑CCCTGCCTCACGTATATACTTTTGCTCTTTCGATAtcatt                                                                                               <  2:3006050/140‑1 (MQ=255)

                                          ggCCGACAGATCGTCGGCCACATTATTTTTTACGTCGACGAATCCTCTTCCCGCTGTTTCGCCCCATATTTCCTTATCCGCATAGTATCAGGTGCGC‑TCC‑CCCTGCCTCACGTATATACTTTTGCTCTTTCGATAtcatt                                                                                               <  1:6616539/140‑1 (MQ=255)

                                          ggCCGACAGATCGTCGGCCACATTATTTTGTACGTCGCCGAATCCTCTTCCCGCTGTTTCGCCCCATATTTCCGTATCCGCATAGGATCAGGTGCGC‑TCC‑CCCTGCCTCACGTATATACTTTTGCTCTTTCGATAtcatt                                                                                               <  2:6151620/140‑1 (MQ=255)

                                           gCCGACAGATCGTCGGCCCCATTATTTTTTACGTCGACGAATCCTCTTCCCGCTGTTTCGCCCCATATTTCCTTATCCGCATAGTATCAGGTGCGC‑TCC‑CCCTGCCTCACGTATATACTTTTGCTCTTTCGATAtcattc                                                                                              >  2:8103729/1‑140 (MQ=255)

                                           gCCGACAGATCGTCGGCCCCATTATTTTTTACGTCGACGAATCCTCTTCCCGCTGTTTCGCCCCATATTTCCTTATCCGCATAGTATCAGGTGCGC‑TCC‑CCCTGCCTCACGTATATACTTTTGCTCTTTCGATAtcattc                                                                                              >  2:5524192/1‑140 (MQ=255)

                                            ccGACAGATCGTCGGCCACATTATTTTTTACGTCGACGAATCCTCTTCCCGCTGTTTCGCCCCATATTTCCTTATCCGCATAGTATCAGGTGCGC‑TCC‑CCCTGCCTCACGTATATACTTTTGCTCTTTCGATAtcattca                                                                                             >  2:4599480/1‑140 (MQ=255)

                                               aCAGATCGTCGGCCACATTATTTTTTACGTCGACGAATCCTCTTCCCGCTGTTTCGCCCCATATTTCCTTATCCGCATAGTATCAGGTGCGC‑TCC‑CCCTGCCTCACGTATATACTTTTGCTCTTTCGATATCATTCatat                                                                                          <  1:431265/140‑1 (MQ=255)

                                                   aTCGTCGGCCACATTATTTTTTACGTCGACGAATCCTCTTCCCGCTGTTTCGCCCCATATTTCCTTATCCGCATAGTATCAGGTGCGC‑TCC‑CCCTGCCTCACGTATATACTTTTGCTCTTTCGATATCATTCatatcaat                                                                                      >  2:7560011/1‑140 (MQ=255)

                                                   aTCGTCGGCCACATTATTTTTTACGTCGACGAATCCTCTTCCCGCTGTTTCGCCCCATATTTCCTTATCCGCATAGTATCAGGTGCGC‑TCC‑CCCTGCCTCACGTATATACTTTTGCTCTTTCGATATCATTCatatcaat                                                                                      >  1:1204619/1‑140 (MQ=255)

                                                    tcgtcgGCCACATTATTTTTTACGTCGACGAATCCTCTTCCCGCTGTTTCGCCCCATATTTCCTTATCCGCATAGTATCAGGTGCGC‑TCC‑CCCTGCCTCACGTATATACTTTTGCTCTTTCGATATCATTCatatcaata                                                                                     <  2:4418557/140‑1 (MQ=255)

                                                    tcgtcgGCCACATTATTTTTTACGTCGACGAATCCTCTTCCCGCTGTTTCGCCCCATATTTCCTTATCCGCATAGTATCAGGTGCGC‑TCC‑CCCTGCCTCACGTATATACTTTTGCTCTTTCGATATCATTCatatcaata                                                                                     <  1:5828164/140‑1 (MQ=255)

                                                          gCCACATTATTTTTTACGTCGACGAATCCTCTTCCCGCTGTTTCGCCCCATATTTCCTTATCCGCATAGTATCAGGTGCGC‑TCC‑CCCTGCCTCACGTATATACTTTTGCTCTTTCGATATCATTCATATCAATATCATGc                                                                               >  1:4685091/1‑140 (MQ=255)

                                                              caTTATTTTTTACGTCGACGAATCCTCTTCCCGCTGTTTCGCCCCATATTTCCTTATCCGCATAGTATCAGGTGCGC‑TCC‑CCCTGCCTCACGTATATACTTTTGCTCTTTCGATATCATTCATATCAATATCATGCaaaa                                                                           >  2:1042530/1‑140 (MQ=255)

                                                                  attTTTTACGTCGACGAATCCTCTTCCCTCTGTTTCGCCCCATATTTCCTTATCCGCATAGTATCAGGTGCGC‑TCC‑CCCTGCCTCACGTATATACTTTTGCTCTTTCGATATCATTCATATCAATATCATGCAAAAGaaa                                                                       <  1:509475/140‑1 (MQ=255)

                                                                  attTTTTACGTCGACGAATCCTCTTCCCGCTGTTTCGCCCCATATTTCCTTATCCGCATAGTATCAGGTGCGC‑TCC‑CCCTGCCTCACGTATATACTTTTGCTCTTTCGATATCATTCATATCAATATCATGCAAAAGaaa                                                                       <  1:6745676/140‑1 (MQ=255)

                                                                        tACGTCGACGAATCCTCTTCCCGCTGTTTCGCCCCATATTTCCTTATCCGCATAGTATCAGGTGCGC‑TCC‑CCCTGCCTCACGTATATACTTTTGCTCTTTCGATATCATTCATATCAATATCATGCAAAAGAAATCAAtt                                                                 >  2:653035/1‑140 (MQ=255)

                                                                         aCGTCGACGAATCCTCTTCCCGCTGTTTCGCCCCATATTTCCTTATCCGCATAGTATCAGGTGCGC‑TCC‑CCCTGCCTCACGTATATACTTTTGCTCTTTCGATATCATTCATATCAATATCATGCAAAAGAAATCAAttt                                                                >  1:3251542/1‑140 (MQ=255)

                                                                         aCGTCGACGAATCCTCTTCCCGCTGTTTCGCCCCATATTTCCTAATCCGCATAGGATCAGGTGCGC‑TCC‑CCCTGCCTCACGTATATACTTTTGCTCTTTCGATATCATTCATATCAATATCATGCAAAAGAAATCAAttt                                                                >  2:1953119/1‑140 (MQ=255)

                                                                             cgacgaATCCTCTTCCCGCTGTTTCGCCCCATATTTCCTTATCCGCATAGTATCAGGTGCGC‑TCC‑CCCTGCCTCACGTATATACTTTTGCTCTTTCGATATCATTCATATCAATATCATGCAAAAGAAATCAATTTACGt                                                            >  1:2878075/1‑140 (MQ=255)

                                                                              gacgaATCCTCTTCCCGCTGTTTCGCCCCATATTTCCTTATCCGCATAGTATCAGGTGCGC‑TCC‑CCCTGCCTCACGTATATACTTTTGCTCTTTCGATATCATTCATATCAATATCATGCAAAAGAAATCAATTTACGtt                                                           <  2:89970/140‑1 (MQ=255)

                                                                              gacgaATCCTCTTCCCGCTGTTTCGCCCCATATTTCCTTATCCGCATAGTATCAGGTGCGC‑TCC‑CCCTGCCTCACGTATATACTTTTGCTCTTTCGATATCATTCATATCAATATCATGCAAAAGAAATCAATTTACGtt                                                           <  2:2546235/140‑1 (MQ=255)

                                                                                         ttCCCGCTGTTTCGCCCCATATTTCCTTATCCGCATAGTATCAGGTGCGC‑TCC‑CCCTGCCTCACGTATATACTTTTGCTCTTTCGATATCATTCATATCAATATCATGCAAAAGAAATCAATTTACGTTGCCTACAcggg                                                <  1:7377737/140‑1 (MQ=255)

                                                                                         ttCCCGCTGTTTCGCCCCATATTTCCTTATCCGCATAGTATCAGGTGCGC‑TCC‑CCCTGCCTCACGTATATACTTTTGCTCTTTCGATATCATTCATATCAATATCATGCAAAAGAAATCAATTTACGTTGCCTACAcggg                                                >  1:9241121/1‑140 (MQ=255)

                                                                                         ttCCCGCTGTTTCGCCCCATATTTCCTTATCCGCATAGTATCAGGTGCGC‑TCC‑CCCTGCCTCACGTATATACTTTTGCTCTTTCGATATCATTCATATCAATATCATGCAAAAGAAATCAATTTACGTTGCCTACAcggg                                                <  1:2866049/140‑1 (MQ=255)

                                                                                            ccGCTGTTTCGCCCCATATTTCCTTATCCGCATAGTATCAGGTGCGC‑TCC‑CCCTGCCTCACGTATATACTTTTGCTCTTTCGATATCATTCATATCAATATCATGCAAAAGAAATCAATTTACGTTGCCTACAcgggcgg                                             <  2:8620485/140‑1 (MQ=255)

                                                                                             cGCTGTTTCGCCCCATATTTCCTTATCCGCATAGTATCAGGTGCGC‑TCC‑CCCTGCCTCACGTATATACTTTTGCTCTTTCGATATCATTCATATCAATATCATGCAAAAGAAATCAATGTACGTTGCCTACAcgggcggg                                            <  2:2876704/140‑1 (MQ=255)

                                                                                              gCTGTTTCGCCCCATATTTCCTTATCCGCATAGTATCAGGTGCGC‑TCC‑CCCTGCCTCACGTATATACTTTTGCTCTTTCGATATCATTCATATCAATATCATGCAAAAGAAATCAATTTACGTTGCCTACACGGGCGGGa                                           >  1:6461696/1‑140 (MQ=255)

                                                                                               cTGTTTCGCCCCATATTTCCTTATCCGCATAGTATCAGGTGCGC‑TCC‑CCCTGCCTCACGTATATACTTTTGCTCTTTCGATATCATTCATATCAATATCATGCAAAAGAAATCAATTTACGTTGCCTACACGGGCGGGAc                                          >  2:602563/1‑140 (MQ=255)

                                                                                                 gTTTCGCCCCATATTTCCTTATCCGCATAGTATCAGGTGCGC‑TCC‑CCCTGCCTCACGTATATACTTTTGCTCTTTCGATATCATTCATATCAATATCATGCAAAAGAAATCAATTTACGTTGCCTACACGGGCGGGACCa                                        >  1:3948541/1‑140 (MQ=255)

                                                                                                 gTTTCGCCCCATATTTCCTTATCCGCATAGTATCAGGTGCGC‑TCC‑CCCTGCCTCACGTATATACTTTTGCTCTTTCGATATCATTCATATCAATATCATGCAAAAGAAATCAATTTACGTTGCCTAAACGGGCGGGACCa                                        >  2:3292600/1‑140 (MQ=255)

                                                                                                 gTTTCGCCCCATATTTCCTTATCCGCAGAGTATCAGGTGCGC‑TCC‑CCCTGCCTCACGTATATACTTTTGCTCTTTCGATATCATTCATATCAATATCATGCAAAAGAAATCAATTTACGTTGCCTACACGGGCGGGACCa                                        <  2:6269233/140‑1 (MQ=255)

                                                                                                                tCCTTATCCGCATAGTATCAGGTGCGC‑TCC‑CCCTGCCTCACGTATATACTTTTGCTCTTTCGATATCATTCATATCAATATCATGCAAAAGAAATCAATTTACGTTGCCTACACGGGCGGGACCATCGGGATGCAGCGtt                         <  2:1246500/140‑1 (MQ=255)

                                                                                                                    tATCCGCATAGTATCAGGTGCGC‑TCC‑CCCTGCCTCACGTATATACTTTTg                                                                                                               >  2:8007330/1‑50 (MQ=255)

                                                                                                                    tATCCGCATAGTATCAGGTGCGC‑TCC‑CCCTGCCTCACGTATATACTTTTGCTCTTTCGATATCATTCATATCAATATCATGCAAAAGAAATCAATTTACGTTGCCTACACGGGCGGGACCATCGGGATGCAGCGTTCCGa                     >  1:7572641/1‑140 (MQ=255)

                                                                                                                     aTCCGCATAGTATCAGGTGCGC‑TCC‑CCCTGCCTCACGTATATACTTTTGCTCTTTCGATATCATTCATATCAATATCATGCAAAAGAAATCAATTTACGTTGCCTACACGGGCGGGACCATCGGGATGCAGCGTTCCGAg                    <  2:3420793/140‑1 (MQ=255)

                                                                                                                     aTCCGCATAGTATCAGGTGCGC‑TCC‑CCCTGCCTCACGTATATACTTTTGCTCTTTCGATATCATTCATATCAATATCATGCAAAAGAAATCAATTTACGTTGCCTACACGGGCGGGACCATCGGGATGCAGCGTTCCGAg                    <  1:651378/140‑1 (MQ=255)

                                                                                                                     aTCCGCATAGTATCAGGTGCGC‑TCC‑CCCTGCCTCACGTATATACTTTTGCTCTTTCGATATCATTCATATCAATATCATGCAAAAGAAATCAATTTACGTTGCCTACACGGGCGGGACCATCGGGATGCAGCGTTCCGAg                    <  1:2266054/140‑1 (MQ=255)

                                                                                                                     aTCCGCATAGTATCAGGTGCGC‑TCC‑CCCTGCCTCACGTATATACTTTTGCTCTTTCGATATCATTCATATCAATATCATGCAAAAGAAATCAATTTACGTTGCCTACACGGGCGGGACCATCGGGATGCAGCGTTCCGAg                    <  1:975787/140‑1 (MQ=255)

                                                                                                                      tCCGCATAGTATCAGGTGCGC‑TCC‑CCCTGCCTCACGTATATACTTTTGCTCTTTCGATATCATTCatat                                                                                          >  1:7098828/1‑69 (MQ=255)

                                                                                                                      tCCGCATAGTATCAGGTGCGC‑TCC‑CCCTGCCTCACGTATATACTTTTGCTCTTTCGATATCATTCATATCAATATCATGCAAAAGAAATCAATTTACGTTGCCTACACGGGCGGGACCATCGGGATGCAGCGTTCCGAGc                   >  2:7176347/1‑140 (MQ=255)

                                                                                                                      tCCGCATAGTATCAGGTGCGC‑TCC‑CCCTGCCTCACGTATATACTTTTGCTCTTTCGATATCATTCATATCAATATCATGCAAAAGAAATCAATTTACGTTGCCTACACGGGCGGGACCATCGGGATGCAGCGTTCCGAGc                   >  1:1367657/1‑140 (MQ=255)

                                                                                                                      tCCGCATAGTATCAGGTGCGC‑TCC‑CCCTGCCTCACGTATATACTTTTGCTCTTTCGATATCATTCATATCAATATCATGCAAAAGAAATCAATTTACGTTGCCTACACGGGCGGGACCATCGGGATGCAGCGTTCCGAGc                   >  1:5603137/1‑140 (MQ=255)

                                                                                                                      tCCGCATAGTATCAGGTGCGC‑TCC‑CCCTGCCTCACGTATATACTTTTGCTCTTTCGATATCATTAATATCAATATCATGCAAAAGAAATCAATTTACGTTGCCTACACGGGCGGGACCATCGGGATGCAGCGTTCCGAGc                   >  1:4917341/1‑140 (MQ=255)

                                                                                                                       ccGCATAGTATCAGGTGCTC‑TCC‑CCCTGCCTCACGTATATACTTTTGCTCTTTCGATATCATTCATATCAATATCATGCAAAAGAAATCACTTTACGTTGCCTACACGGGCGGGACCATCGGGATGCAGCGTTCCGAGCa                  <  2:4703483/140‑1 (MQ=255)

                                                                                                                       ccGCATAGTATCAGGTGCGC‑TCC‑CCCTGCCTCACGTATATACTTTTGCTCTTTCGATATCATTCATATCAATATCATGCAAAAGAAATCAATTTACGTTGCCTACACGGGCGGGACCATCGGGATGCAGCGTTCCGAGCa                  <  1:6176250/140‑1 (MQ=255)

                                                                                                                       ccGCATAGTATCAGGTGCGC‑TCC‑CCCTGCCTCACGTATATACTTTTGCTCTTTCGATATCATTCATATCAATATCATGCAAAAGAAATCAATTTACGTTGCCTACACGGGCGGGACCATCGGGATGCAGCGTTCCGAGCa                  <  1:3479330/140‑1 (MQ=255)

                                                                                                                       ccGCATAGTATCAGGTGCGC‑TCC‑CCCTGCCTCACGTATATACTTTTGCTCTTTCGATATCATTCATATCAATATCATGCAAAAGAAATCAATTTACGTTGCCTACACGGGCGGGACCATCGGGATGCAGCGTTCCGAGCa                  <  2:1837196/140‑1 (MQ=255)

                                                                                                                       ccGCATAGTATCAGGTGCGC‑TCC‑CCCTGCCTCACGTATATACTTTTGCTCTTTCGATATCATTCATATCAATATCATGCAAAAGAAATCAATTTACGTTGCCTACACGGGCGGGACCATCGGGATGCAGCGTTCCGAGCa                  <  1:8422077/140‑1 (MQ=255)

                                                                                                                       ccGCATAGTATCAGGTGCGC‑TCC‑CCCTGCCTCACGTATATACTTTTGCTCTTTCGATATCATTCATATCAATATCATGCAAAAGAAATCAATTTACGTTGCCTACACGGGCGGGACCATCGGGATGCAGCGTTCCGAGCa                  <  1:5771937/140‑1 (MQ=255)

                                                                                                                       ccGCATAGTATCAGGTGCGC‑TCC‑CCCTGCCTCACGTATATACTTTTGCTCTTTCGATATCATTCATATCAATATCATGCAAAAGAAATCAATTTACGTTGCCTACACGGGCGGGACCATCGGGATGCAGCGTTCCGAGCa                  <  1:8257017/140‑1 (MQ=255)

                                                                                                                       ccGCATAGTATCAGGTGCGC‑TCC‑CCCTGCCTCACGTATATACTTTTGCTCTTTCGATATCATTCATATCAATATCATGCAAAAGAAATCAATTTACGTTGCCTACACGGGCGGGACCATCGGGATGCAGCGTTCCGAGCa                  <  1:1637672/140‑1 (MQ=255)

                                                                                                                       ccGCATAGTATCAGGTGCGC‑TCC‑CCCTGCCTCACGTATATACTTTTGCTCTTTCGATATCATTCATATCAATATCATGCAAAAGAAATCAATTTACGTTGCCTACACGGGCGGGACCATCGGGATGCAGCGTTCCGAGCa                  <  1:3646481/140‑1 (MQ=255)

                                                                                                                       ccGCATAGTATCAGGTGCGC‑TCC‑CCCTGCCTCACGTATATACTTTTGCTCTTTCGATATCATTCATATCAATATCATGCAAAAGAAATCAATTTACGTTGCCTACACGGGCGGGACCATCGGGATGCAGCGTTCCGAGCa                  <  1:7974653/140‑1 (MQ=255)

                                                                                                                       ccGCATAGTATCAGGTGCGC‑TCC‑CCCTGCCTCACGTATATACTTTTGCTCTTTCGATATCATTCATATCAATATCATGCAAAAGAAATCAATTTACGTTGCCTACACGGGCGGGACCATCGGGATGCAGCGTTCCGAGCa                  <  1:4846581/140‑1 (MQ=255)

                                                                                                                        cGCATAGTATCAGGTGCGC‑TCC‑CCCTGCCTCACGTATATACTTTTGCTCTTTAGATATCATTCATATCAAGATCATGCAAAAGAAATCAATTTACGTTGCCTACACGGGCGGGACCATCGGGATGCAGCGTTCCGAGCAg                 <  2:8442013/140‑1 (MQ=255)

                                                                                                                           aTAGTATCAGGTGCGC‑TCC‑CCCTGCCTCACGTATATACTTTTGCTCTTTCGATATCATTCATATCAATATCATGCAAAAGAAATCAATTTACGTTGCCTACACGGGCGGGACCATCGGGATGCAGCGTTCCGAGCAggg               <  2:4472747/139‑1 (MQ=255)

                                                                                                                             aGTATCAGGTGCGC‑TCC‑CCCTGCCTCACGTATATACTTTTGCTCTTTCGATATCATTCATATCAATATCATGCAAAAGAAATCAATTTACGTTGCCTACACGGGCGGGACCATCGGGATGCAGCGTTCCGAGCAGGGTta            <  2:3156110/140‑1 (MQ=255)

                                                                                                                               tATCAGGTGCGC‑TCC‑CCCTGCCTCACGTATATACTTTTGCTCTTTCGATATCATTCATATCAATATCATGCAAAAGAAATCAATTTACGTTGCCTACACGGGCGGGACCATCGGGATGCAGCGTTCCGAGCAGGGTtata          >  1:2170339/1‑140 (MQ=255)

                                                                                                                               tATCAGGTGCGC‑TCC‑CCCTGCCTCACGTATATACTTTTGCTCTTTCGATATCATTCATATCAATATCATGCAAAAGAAATCAATTTACGTTGCCTACACGGGCGGGACCATCGGGATGCAGCGTTCCGAGCAGGGTtata          >  1:8518439/1‑140 (MQ=255)

                                                                                                                                aTCAGGTGCGC‑TCC‑CCCTGCCTCACGTATATACTTTTGCTCTTTCGATATCATTCATATCAATATCATGCAAAAGAAATCAATTTACGTTGCCTACACGGGCGGGACCATCGGGATGCAGCGTTCCGAGCAGGGTtatat         >  2:4163691/1‑140 (MQ=255)

                                                                                                                                 tCAGGTGCGC‑TCC‑CCCTGCCTCACGTATATACTTTTGCTCTTTCGATATCATTCATATCAATATCATGCAAAAGAAATCAATTTACGTTGCCTACACGGGCGGGACCATCGGGATGCAGCGTTCCGAGCAGGGTtatata        <  2:825422/140‑1 (MQ=255)

                                                                                                                                 tCAGGTGCGC‑TCC‑CCCTGCCTCACGTATATACTTTTGCTCTTTCGATATCATTCATATCAATATCATGCAAAAGAAATCAATTTACGTTGCCTACACGGGCGGGACCATCGGGATGCAGCGTTCCGAGCAGGGTtatata        >  2:2020165/1‑140 (MQ=255)

                                                                                                                                       gcgc‑TCC‑CCCTGCCTCACGTATATACTTTTGCTCTTTCGATATCATTCATATCAATATCATGCAAAAGAAATCAATTTACGTTGCCTACACGGGCGGGACCATCGGGATGCAGCGTTCCGAGCAGGGTTATATACCGgtg  >  2:4027551/1‑140 (MQ=255)

                                                                                                                                       gcgc‑TCC‑CCCTGCCTCACGTATATACTTTTGCTCTTTCGATATCATTCATATCAATATCATGCAAAAGAAATCAATTTACGTTGCCTACACGGGCGGGACCATCGGGATGCAGCGTTCCGAGCAGGGTTATATACCGgtg  >  2:8506454/1‑140 (MQ=255)

                                                                                                                                       gcgc‑TCC‑CCCTGCCTCACGTATATACTTTTGCTCTTTCGATATCATTCATATCAATATCATGCAAAAGAAATCAATTTACGTTGCCTACACGGGCGGGACCATCGGGATGCAGCGTTCCGAGCAGGGTTATATACCGgtg  >  2:5094916/1‑140 (MQ=255)

TTGCCTGACCTGCGCCAACATGCGTTAAGTCTTGTACTGAGTGGCCGACAGATCGTCGGCCACATTATTTTTTACGTCGACGAATCCTCTTCCCGCTGTTTCGCCCCATATTTCCTTATCCGCATAGTATCAGGTGCGC‑T‑C‑CCCTGCCTCACGTATATACTTTTGCTCTTTCGATATCATTCATATCAATATCATGCAAAAGAAATCAATTTACGTTGCCTACACGGGCGGGACCATCGGGATGCAGCGTTCCGAGCAGGGTTATATACCGGTG  >  NZ_CP025268/1855482‑1855755

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`