BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP025268	1,913,492	+T	coding (88/144 nt)	mgrB ←	PhoP/PhoQ regulator MgrB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP025268	1,913,492	1	.	T	99.3%	2183.6 / 3.2	557	coding (88/144 nt)	mgrB	PhoP/PhoQ regulator MgrB
Reads supporting (aligned to +/- strand): ref base . (2/2); new base T (329/224); total (331/226)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 588 total aligned reads displayed.

TTCGTTCATTTCACCACCTCTGAGTAGAGGCGCTATTCTACCACTGCTGGAGAGGAAGAAAATCTAGTGCTGAAAAAATGATATCACCACGGGATAAACTGGTTAATGGCACAAATTCCGCTGAAAAATTGTACATCC‑TG‑ATCGCACATCATGTTGAATACCTGCGCCCAAAGCAGCAAGCAAGCCAACACCACGACAACCAGAACGACCCATCGAAACTTTTTCACTCCAATCTCCGTTTCACCTACCTTATGTCATTAAACTAGCATGCGT  >  NZ_CP025268/1913353‑1913625

  cGTTCATTTCACCACCTCTGAGTAGAGGCGCTATTCTACCACTGCTGGAGAGGAAGAAAATCTAGTGCTGAAAAAATGATATCACCACGGGATAAACTGGTTAATGGCACAAATTCCGCTGAAAAATTGTACATCC‑Tgta                                                                                                                                      >  1:8362992/1‑138 (MQ=255)

    ttCATTTCACCACCTCTGAGTAGAGGCGCTATTCTACCACTGCTGGAGAGGAAGAAAATCTAGTGCTGAAAAAATGATATCACCACGGGATAAACTGGTTAATGGCACAAATTCCGCTGAAAAATTGTACATCC‑Tgtatc                                                                                                                                    <  1:8413283/140‑5 (MQ=255)

      cATTTCACCACCTCTGAGTAGAGGCGCTATTCTACCACTGCTGGAGAGGAAGAAAATCTAGTGCTGAAAAAATGATATCACCACGGGATAAACTGGTTAATGGCACAAATTCCGCTGAAAAATTGTACATCC‑TGTATCGc                                                                                                                                  <  1:600432/140‑1 (MQ=255)

       aTTTCACCACCTCTGAGTAGAGGCGCTATTCTACCACTGCTGGAGAGGAAGAAAATCTAGTGCTGAAAAAATGATATCACCACGGGATAAACTGGTTAATGGCACAAATTCCGCTGAAAAATTGTACATCC‑TGTATCGca                                                                                                                                 <  2:4074659/140‑1 (MQ=255)

        tttCACCACCTCTGAGTAGAGGCGCTATTCTACCACTGCTGGAGAGGAAGAAAATCTAGTGCTGAAAAAATGATATCACCACGGGATAAACTGGTTAATGGCACAAATTCCGCTGAAAAATTGTACATCC‑TGTATCGcac                                                                                                                                <  1:1221120/140‑1 (MQ=255)

         ttCACCACCTCTGAGTAGAGGCGCTATTCTACCACTGCTGGAGAGGAAGAAAATCTAGTGCTGAAAAAATGATATCACCACGGGATAAACTGGTTAATGGCACAAATTCCGCTGAAAAATTGTACATCC‑TGTATCGcaca                                                                                                                               <  1:3212787/140‑1 (MQ=255)

           caccacCTCTGAGTAGAGGCGCTATTCTACCACTGCTGGAGAGGAAGAAAATCTAGTGCTGAAAAAATGATATCACCACGGGATAAACTGGTTAATGGCACAAATTCCGCTGAAAAATTGTACATCC‑TGTATCGCAcatc                                                                                                                             <  2:457183/140‑1 (MQ=255)

           caccacCTCTGAGTAGAGGCGCTATTCTACCACTGCTGGAGAGGAAGAAAATCTAGTGCTGAAAAAATGATATCACCACGGGATAAACTGGTTAATGGCACAAATTCCGCTGAAAAATTGTACATCC‑TGTATCGCAcatc                                                                                                                             <  2:3698101/140‑1 (MQ=255)

            accaccTCTGAGTAGAGGCGCTATTCTACCACTGCTGGAGAGGAAGAAAATCTAGTGCTGAAAAAATGATATCACCACGGGATAAACTGGTTAATGGCACAAATTCCGCTGAAAAATTGTACATCC‑TGTATCGCAcatca                                                                                                                            <  1:7275336/140‑1 (MQ=255)

            accaccTCTGAGTAGAGGCGCTATTCTACCACTGCTGGAGAGGAAGAAAATCTAGTGCTGAAAAAATGATATCACCACGGGATAAACTGGTTAATGGCACAAATTCCGCTGAAAAATTGTACATCC‑TGTATCGCAcatca                                                                                                                            <  1:4385087/140‑1 (MQ=255)

                 ctctGAGTAGAGGCGCTATTCTACCACTGCTGGAGAGGAAGAAAATCTAGTGCTGAAAAAATGATATCACAACGGGATAAACTGGTTAATGGCACAAATTAAGCTGAAAAATTGTACATCC‑TGTATCGCACATCATGTTg                                                                                                                       >  2:7312297/1‑140 (MQ=255)

                   ctGAGTAGAGGCGGTATTCTACCACTGCTGGAGAGGAAGAAAATCTAGTGCTGAAAAAATGATATCACCACGGGATAAACTGGTTAATGGCACAAATTCCGCTGAAAAATTGTACATCC‑TGTATCGCACATCATGTTGaa                                                                                                                     >  1:6426110/1‑140 (MQ=255)

                     gAGTAGAGGCGCTATTCTACCACTGCTGGAGAGGAAGAAAATCTAGTGCTGAAAAAATGATATCACCACGGGATAAACTGGTTAATGGCACAAATTCCGCTGAAAAATTGTACATCC‑TGTATCGCACATCATGGTGAATa                                                                                                                   >  1:8222536/1‑140 (MQ=255)

                      aGTAGAGGCGCTATTCTACCACTGCTGGAGAGGAAGAAAATCTAGTGCTGAAAAAATGATATCACCACGGGATAAACTGGTTAATGGCACAAATTCCGCTGAAAAATTGTACATCC‑TGTATCGCACATCATGTTGAATAc                                                                                                                  >  2:6091065/1‑140 (MQ=255)

                        tAGAGGCGCTATTCTACCACTGCTGGAGAGGAAGAAAATCTAGTGCTGAAAAAATGATATCACCACGGGATAAACTGGTTAATGGCACAAATTCCGCTGAAAAATTGTACATCC‑TGTATCGCACATCATGTTGAATACCt                                                                                                                <  2:1388199/140‑1 (MQ=255)

                          gagGCGCTATTCTACCACTGCTGGAGAGGAAGAAAATCTAGTGCTGAAAAAATGATATCACCACGGGATAAACTGGTTAATGGCACAAATTCCGCTGAAAAATTGTACATCC‑TGTATCGCACATCATGTTGAATACCTgc                                                                                                              >  1:5196341/1‑140 (MQ=255)

                           agtctcTATTCTACCACTGCTGGAGAGGAAGAAAATCAAGTTCTGAAAAACTGTTATCAACAAGGTATACACAGGTTAATGTCACAAATTACGCTGAAAACTAGTACAACCTTT‑ATCTCTCATCATGTTTAATAACTgag                                                                                                             >  2:7015087/6‑138 (MQ=255)

                           agGCGCTATTCTACCACTGCTGGAGAGGAAGAAAATCTAGTGCTGAAAAAATGATATCACCACGGGATAAACTGGTTAATGGCACAAATTCCGCTGAAAAATTGTACATCC‑TGTATCGCACCTCATGTTGAATACCTgcg                                                                                                             >  2:888238/1‑140 (MQ=255)

                           agGCGCTATTCTACCACTGCTGGAGAGGAAGAAAATCTAGTGCTGAAAAAATGATATCACCACGGGATAAACTGGTTAATGGCACAAATTCCGCTGAAAAATTGTACATCC‑TGTATCGCACATCATGTTGAATACCTgcg                                                                                                             >  2:3350300/1‑140 (MQ=255)

                           agGCGCTATTCTACCACTGCTGGAGAGGAAGAAAATCTAGTGCTGAAAAAATGATATCACCACGGGATAAACTGGTTAATGGCACAAATTCCGCTGAAAAATTGTACATCC‑TGTATCGCACATCATGTTGAATACCTgcg                                                                                                             >  1:6141824/1‑140 (MQ=255)

                            ggCGCGATTCTACCACTGCTGGAGAGGAAGAACATCTAGTGCTGAAAAAATGATATCACCACGGGATAAAATGGTTAATGGCACAAATTCCGCTGAAAAATTGTACATCC‑TGTATCGCACATCATGTTGAATACCTgcgc                                                                                                            >  2:3959921/1‑140 (MQ=255)

                             gcgcTATTCTACCACTGCTGGAGAGGAAGAAAATCTAGTGCTGAAAAAATGATATCACCACGGGATAAACTGGTTAATGGCACAAATTCCGCTGAAAAATTGTACATCC‑TGTATCGCACATCATGTTGAATACCTGCGcc                                                                                                           >  2:8375997/1‑140 (MQ=255)

                             gcgcTATTCTACCACTGCTGGAGAGGAAGAAAATCTAGTGCTGAAAAAATGATATCACCACGGGATAAAATGGTTAATGGCACAAATTCCGCTGAAAAATTGTACATCC‑TGTATCGCACATCATGTTGAATACCTGCGcc                                                                                                           >  1:2250563/1‑140 (MQ=255)

                                cTATTCTACCACTGCTGGAGAGGAAGAAAATCTAGTGCTGAAAAAATGATATCACCACGGGATAAACTGGTTAATGGCACAAATTCCGCTGAAAAATTGTACATCC‑TGTATCGCACATCATGTTGAATACCTGCGCCCaa                                                                                                        >  2:1881181/1‑140 (MQ=255)

                                cTATTCTACCACTGCTGGAGAGGAAGAAAATCTAGTGCTGAAAAAATGATATCACCACGGGATAAACTGGTTAATGGCACAAATTCCGCTGAAAAATTGTACATCC‑TGTATCGCACATCATGTTGAATACCTGCGCCCaa                                                                                                        >  2:7948196/1‑140 (MQ=255)

                                cTATTCTACCACTGCTGGAGAGGAAGAAAATCTAGTGCTGAAAAAATGATATCACCACGGGATAAACTGGTTAATGGCACAAATTCCGCTGAAAAATTGTACATCC‑TGTATCGCACATCATGTTGAATACCTGCGCCCaa                                                                                                        >  1:243613/1‑140 (MQ=255)

                                  aTTCTACCACTGCTGGAGAGGAAGAAAATCTAGTGCTGAAAAAATGATATCACCACGGGATAAACTGGTTAATGGCACAAATTCCGCTGAAAAATTGTACATCC‑TGTATCGCACATCATGTTGAATACCTGCGCCCAAag                                                                                                      >  2:5233306/1‑140 (MQ=255)

                                        ccACTGCTGGAGAGGAAGAAAATCTAGTGCTGAAAAAATGATATCACCACGGGATAAACTGGTTAATGGCACAAATTCCGCTGAAAAATTGTACATCC‑TGTATCGCACATCATGTTGAATACCTGCGCCCAAAGCagcaa                                                                                                >  1:1699494/1‑140 (MQ=255)

                                         cACTGCTGGAGAGGAAGAAAATCTAGTGCTGAAAAAATGATATCACCACGGGATAAACTGGTTAATGGCACAAATTCCGCTGAAAAATTGTACATCC‑TGTATCGCACATCATGTTGAATACCTGCGCCCAAAGCagcaag                                                                                               <  1:6964962/140‑1 (MQ=255)

                                          aCTGCTGGAGAGGAAGAAAATCTAGTGCTGAAAAAATGATATCACCACGGGATAAACTGGTTAATGGCACAAATTCCGCTGAAAAATTGTACATCC‑TGTATCGCACATCATGTTGAATACCTGCGCCCAAAGCagcaagc                                                                                              <  2:2740613/140‑1 (MQ=255)

                                           ctgctgGAGAGGAAGAAAATCTAGTGCTGAAAAAATGATATCACCACGGGATAAACTGGTTAATGGCACAAATTCCGCTGAAAAATTGTACATCC‑TGTATCGCAcatc                                                                                                                             >  2:6633589/1‑108 (MQ=255)

                                           ctgctgGAGAGGAAGAAAATCTAGTGCTGAAAAAATGATATCACCACGGGATAAACTGGTTAATGGCACAAATTCCGCTGAAAAATTGTACATCC‑TGTATCGCAcatc                                                                                                                             <  1:6633589/108‑1 (MQ=255)

                                           ctgctgGAGAGGAAGAAAATCTAGTGCTGAAAAAATGATATCACCACGGGATAAACTGGTTAATGGCACAAATTCCGCTGAAAAATTGTACATCC‑TGTATCGCACATCATGTTGAATACCTGCGCCCAAAGCagcaagca                                                                                             >  2:6190991/1‑140 (MQ=255)

                                           ctgctgGAGAGGAAGAAAATCTAGTGCTGAAAAAATGATATCACCACGGGATAAACTGGTTAATGGCACAAATTCCGCTGAAAAATTGTACATCC‑TGTATCGCACATCATGTTGAATACCTGCGCCCAAAGCagcaagca                                                                                             >  1:1139409/1‑140 (MQ=255)

                                           ctgctgGAGAGGAAGAAAATCTAGTGCTGAAAAAATGATATCACCACGGGATAAACTGGTTAATGGCACAAATTCCGCTGAAAAATTGTACATCC‑TGTATCGCACATCATGTTGAATACCTGCGCCCAAAGCagcaagca                                                                                             >  2:2358242/1‑140 (MQ=255)

                                           ctgctgGAGAGGAAGAAAATCTAGTGCTGAAAAAATGATATCACCACGGGATAAACTGGTTAATGGCACAAATTCCGCTGAAAAATTGTACATCC‑TGTATCGCACATCATGTTGAATACCTGCGCCCAAAGCagcaagca                                                                                             >  2:3480224/1‑140 (MQ=255)

                                           ctgctgGAGAGGAAGAAAATCTAGTGCTGAAAAAATGATATCACCACGGGATAAACTGGTTAATGGCACAAATTCCGCTGAAAAATTGTACATCC‑TGTATCGCACATCATGTTGAATACCTGCGCCCAAAGCagcaagca                                                                                             >  2:688971/1‑140 (MQ=255)

                                           ctgctgGAGAGGAAGAAAATCTAGTGCTGAAAAAATGATATCACCACGGGATAAACTGGTTAATGGCACAAATTCCGCTGAAAAATTGTACATCC‑TGTATCGCACATCATGTTGAATACCTGCGCCCAAAGCagcaagca                                                                                             >  1:3042183/1‑140 (MQ=255)

                                             gctgGAGAGGAAGAAAATCTAGTGCTGAAAAAATGATATCACCACGGGATAAACTGGTTAATGGCACAAATTCCGCTGAAAAATTGTACATCC‑TGTATCGcaca                                                                                                                               <  2:5670231/104‑1 (MQ=255)

                                             gctgGAGAGGAAGAAAATCTAGTGCTGAAAAAATGATATCACCACGGGATAAACTGGTTAATGGCACAAATTCCGCTGAAAAATTGTACATCC‑TGTATCGCACATCATGTTGAATACCTGCGCCCAAagca                                                                                                    <  1:3798639/131‑1 (MQ=255)

                                             gctgGAGAGGAAGAAAATCTAGTGCTGAAAAAATGATATCACCACGGGATAAACTGGTTAATGGCACAAATTCCGCTGAAAAATTGTACATCC‑TGTATCGCACATCATGTTGAATACCTGCGCCCAAAGCAGcaagcaag                                                                                           >  1:7611635/1‑140 (MQ=255)

                                             gctgGAGAGGAAGAAAATCTAGTGCTGAAAAAATGATATCACCACGGGATAAACTGGTTAATGGCACAAATTCCGCTGAAAAATTGTACATCC‑TGTATCGCACATCATGTTGAATACCTGCGCCCAAAGCAGcaagcaag                                                                                           >  1:7728161/1‑140 (MQ=255)

                                             gctgGAGAGGAAGAAAATCTAGTGCTGAAAAAATGATATCACCACGGGATAAACTGGTTAATGGCACAAATTCCGCTGAAAAATTGTACATCC‑TGTATCGCACATCATGTTGAATACCTGCGCCCAAAGCAGcaagcaag                                                                                           >  1:395291/1‑140 (MQ=255)

                                             gctgGAGAGGAAGAAAATCTAGTGCTGAAAAAATGATATCACCACGGGATAAACTGGTTAATGGCACAAATTCCGCTGAAAAATTGTACATCC‑TGTATCGCACATCATGTTGAATACCTGCGCCCAAAGCAGcaagcaag                                                                                           >  1:1024733/1‑140 (MQ=255)

                                             gctgGAGAGGAAGAAAATCTAGTGCTGAAAAAATGATATCACCACGGGATAAACTGGTTAATGGCACAAATTCCGCTGAAAAATTGTACATCC‑TGTATCGCACATCATGTTGAATACCTGCGCCCAAAGCAGcaagcaag                                                                                           >  1:4987343/1‑140 (MQ=255)

                                              ctgGAGAGGAAGAAAATCTAGTGCTGAAAAAATGATATCACCACGGGATAAACTGGTTAATGGCACAAATTCCGCTGAAAAATTGTACATCC‑TGTATCGCACATCATGTTGAATACCTGCGCCCAAAGCAGCaagcaagc                                                                                          >  2:5965656/1‑140 (MQ=255)

                                                ggAGAGGAAGAAAATCTAGTGCTGAAAAAATGATATCACCACGGGATAAACTGGTTAATGGCACAAATTCCGCTGAAAAATTGTACATCC‑TGTATCGCACATCATGTTGAATACCTGCGCCCAAAGCAGCAAGCAAGCCa                                                                                        >  2:4401347/1‑140 (MQ=255)

                                                 gagaGGAATAAAATCTAGTGCTGAAAAAATGATATCACCACGGGATAAACTGGTTAATGGCACAAATTCCGCTGAAAAATTGTACATCC‑TGTATCGCACATCATGTTGAATACCTGCGCCCAAAGCAGCAAGCAAGCCaa                                                                                       <  1:6521307/140‑1 (MQ=255)

                                                 gagaGGAAGAAAATCTAGTGCTGAAAAAATGATATCACCACGGGATAAACTGGTTAATGGCACAAATTCCGCTGAAAAATTGTACATCC‑TGTATCGCACATCATGTTGAATACCTGCGCCCAAAGCAGCAAGCAAGCCaa                                                                                       >  2:5567509/1‑140 (MQ=255)

                                                  agagGAAGAAAATCTAGTGCTGAAAAAATGATATCACCACGGGATAAACTGGTTAATGGCACAAATTCCGCTGAAAAATTGTACATCC‑TGTATCGCACATCATGTTGAATACCTGCGCCCAAAGCAGCAAGCAAGCCAac                                                                                      >  2:750541/1‑140 (MQ=255)

                                                   gagGAAGAAAATCTAGTGCTGAAAAAATGATATCACCACGGGATAAACTGGTTAATGGCACAAATTCCGCTGAAAAATTGTACATCC‑TGTATCGCACATCATGTTGAATACCTGCGCCCAAAGCAGCAAGCAAGCCAaca                                                                                     <  1:337090/140‑1 (MQ=255)

                                                   gagGAAGAAAATCTAGTGCTGAAAAAATGATATCACCACGGGATAAACTGGTTAATGGCACAAATTCCGCTGAAAAATTGTACATCC‑TGTATCGCACATCATGTTGAATACCTGCGCCCAAAGCAGCAAGCAAGCCAaca                                                                                     >  1:1855024/1‑140 (MQ=255)

                                                   gagGAAGAAAATCTAGTGCTGAAAAAATGATATCACCACGGGATAAACTGGTTAATGGCACAAATTCCGCTGAAAAATTGTACATCC‑TGTATCGCACATCATGTTGAATACCTGCGCCCAAAGCAGCAAGCAAGCCAaca                                                                                     <  1:2667217/140‑1 (MQ=255)

                                                      gaagaaAATCTAGTGCTGAAAAAATGATATCACCACGGGATAAACTGGTTAATGGCACNAATTCCGCTGAAAAATTGTACATCC‑TGTATCGCACATCATGTTGAATACCTGCGCCCAAAGCAGCAAGCAAGCCAAcacca                                                                                  >  1:6284939/1‑140 (MQ=255)

                                                      gaagaaAATCTAGTGCTGAAAAAATGATATCACCACGGGATAAACTGGTTAATGGCACAAATTCCGCTGTAAAATTGTACATCC‑TGTATCGCACATCATGTTGAATACCTGCGCCCAAAGCAGCAAGCAAGCCAAcacca                                                                                  >  1:9384351/1‑140 (MQ=255)

                                                        agaaAATCTAGTGCTGAAAAAATGATATCACCACGGGATAAACTGGTTAATGGCACAAATTCCGCTGAAAAATTGTACATCC‑TGTATCGCACATCATGTTGAATACCTGCGCCCAAAGCAGCAAGCAAGCCAACACCACg                                                                                >  1:3603594/1‑140 (MQ=255)

                                                        agaaAATCTAGTGCTGAAAAAATGATATCACCACGGGATAAACTGGTTAATGGCACAAATTCCGCTGAAAAATTGTACATCC‑TGTATCGCACATCATGTTGAATACCTGCGCCCAAAGCAGCAAGCAAGCCAACACCACg                                                                                <  2:26236/140‑1 (MQ=255)

                                                           aaaTCTAGTGCTGAAAAAATGATATCACCACGGGATAAACTGGTTAATGGCACAAATTCCGCTGAAAAATTGTACATCC‑TGTATCGCACATCATGTTGAATACCTGCGCCCAAAGCAGCAAGCAAGCCAACACCACGACa                                                                             <  1:4847232/140‑1 (MQ=255)

                                                           aaaTCTAGTGCTGAAAAAATGATATCACCACGGGATAAACTGGTTAATGGCACAAATTCCGCTGAAAAATTGTACATCC‑TGTATCGCACATCATGTTGAATACCTGCGCCCAAAGCAGCAAGCAAGCCAACACCACGACa                                                                             <  1:3110534/140‑1 (MQ=255)

                                                             aTCTAGTGCTGAAAAAATGATATCACCACGGGATAAACTGGTTAATGGCACAAATTCCGCTGAAAAATTGTACATCC‑TGTATCGCACATCATGTTGAATACCTGCGCCCAAAGCAGCAAGCAAGCCAACACCACGACAAc                                                                           <  2:2958743/140‑1 (MQ=255)

                                                                tAGTGCTGAAAAAATGATATCACCACGGGATAAACTGGTTAATGGCACAAATTCCGCTGAAAAATTGTACATCC‑TGTATCGCACATCATGTTGAATACCTGCGCCCAAAGCAGCAAGCAAGCCAACACCACGACAACCAg                                                                        >  1:5653632/1‑140 (MQ=255)

                                                                   tGCTGAAAAAATGATATCACCACGGGATAAACTGGTTAATGGCACAAATTCCGCTGAAAAATTGTACATCC‑TGTATCGCACATCATGTTGAATACCTGCGCCCAAAGCAGCAAGCAAGCCAACACCACGACAACCAGAAc                                                                     >  1:7045520/1‑140 (MQ=255)

                                                                      tGAAAAAATGATATCACCACGGGATAAACTGGTTAATGGCACAAATTCCGCTGAAAAATTGTACATCC‑TGTATCGCACATCATGTTGAATACCTGCGCCCAAAGCAGCAAGCAAGCCAACACCACGACAACCAGAACGAc                                                                  <  1:5427518/140‑1 (MQ=255)

                                                                                       cacGGGATAAACTGGTTAATGGCACAAATTCCGCTGAAAAATTGTACATCC‑TGTATCGCACATCATGTTGAATACCTGCGCCCAAAGCAGCAAGCAAGCCAACACCACGACAACCAGAACGACCCATCGAAACTTTTTCa                                                 >  2:9220926/1‑140 (MQ=255)

                                                                                         cGGGATAAACTGGTTAATGGCACAAATTCCGCTGAAAAATTGTACATCC‑TGTATCGCACATCATGTTGAATACCTGCGCCCAAAGCAGCAAGCAAGCCAACACCACGACAACCAGAACGACCCATCGAAACTTTTTCACt                                               >  1:4613175/1‑140 (MQ=255)

                                                                                               aaaCTGGTTAATGGCACAAATTCCGCTGAAAAATTGTACATCC‑TGTATCGCACATCATGTTGAATACCTGCGCCCAAAGCAGCAAGCAAGCCAACACCACGACAACCAGAACGACCCATCGAAACTTTTTCACTCCAAtc                                         <  2:3205489/140‑1 (MQ=255)

                                                                                               aaaCTGGTTAATGGCACAAATTCCGCTGAAAAATTGTACATCC‑TGTATCGCACATCATGTTGAATACCTGCGCCCAAAGCAGCAAGCAAGCCAACACCACGACAACCAGAACGACCCATCGAAACTTTTTCACTCCAAtc                                         >  2:6295196/1‑140 (MQ=255)

                                                                                               aaaCTGGTTAATGGCACAAATTCCGCTGAAAAATTGTACATCC‑TGTATCGCACATCATGTTGAATACCTGCGCCCAAAGCAGCAAGCAAGCCAACACCACGACAACCAGAACGACCCATCGAAACTTTTTCACTCCAAtc                                         <  1:9192157/140‑1 (MQ=255)

                                                                                                   tGGTTAATGGCACAAATTCCGCTGAAAAATTGTACATCC‑TGTATCGCACATCATGTTGAATACCTGCGCCCAAAGCAGCAAGCAAGCCAACACCACGACAACCAGAACGACCCATCGAAACTTTTTCACTCCAATCTCCg                                     <  2:5110981/140‑1 (MQ=255)

                                                                                                    ggTTAATGGCACAAATTCCGCTGAAAAATTGTACATCC‑TGTATCGCACATCATGTTGAATACCTGCGCCCAAAGCAGCAAGCAAGCCAACACCACGACAACCAGAACGACCCATCGAAACTTTTTCACTCCAATCTCCGt                                    <  2:5886539/140‑1 (MQ=255)

                                                                                                    ggTTAATGGCACAAATTCCGCTGAAAAATTGTACATCC‑TGTATCGCACATCATGTTGAATACCTGCGCCCAAAGCAGCAAGCAAGCCAACACCACGACAACCAGAACGACCCATCGAAACTTTTTCACTCCAATCTCCGt                                    >  2:4818914/1‑140 (MQ=255)

                                                                                                     gTTAATGGCACAAATTCCGCTGAAAAATTGTACATCC‑TGTATCGCACATCATGTTGAATACCTGCGCCCAAAGCAGCAAGCAAGCCAACACCACGTCAACCAGAACGACCCATCGAATCTTTTTCACTCCAATCTCCGtt                                   >  1:7953687/1‑140 (MQ=255)

                                                                                                     gTTAATGGCACAAATTCCGCTGAAAAATTGTACATCC‑TGTATCGCACATCATGTTGAATACCTGCGCCCAAAGCAGCAAGCAAGCCAACACCACGACAACCAGAACGACCCATCGAAACTTTTTCACTCCAATCTCCGtt                                   <  2:7745614/140‑1 (MQ=255)

                                                                                                     gTTAATGGCACAAATTCCGCTGAAAAATTGTACATCC‑TGTATCGCACATCATGTTGAATACCTGCGCCCAAAGCAGCAAGCAAGCCAACACCACGACAACCAGAACGACCCATCGAAACTTTTTCACTCCAATCTCCGtt                                   >  2:5297029/1‑140 (MQ=255)

                                                                                                     gTTAATGGCACAAATTCCGCTGAAAAATTGTACATCC‑TGTATCGCACATCATGTTGAATACCTGCGCCCAAAGCAGCAAGCAAGCCAACACCACGACAACCAGAACGACCCATCGAAACTTTTTCACTCCAATCTCCGtt                                   <  1:908606/140‑1 (MQ=255)

                                                                                                     gTTAATGGCACAAATTCCGCTGAAAAATTGTACATCC‑TGTATCGCACATCATGTTGAATACCTGCGCCCAAAGCAGCAAGCAAGCCAACACCACGACAACCAGAACGACCCATCGAAACTTTTTCACTCCAATCTCCGtt                                   >  1:282354/1‑140 (MQ=255)

                                                                                                      ttAATGGCACAAATTCCGCTGAAAAATTGTACATCC‑TGTATCGCACATCATGTTGAATACCTGCGCCCAAAGAAGCAAGCAAGCCAACACCACGACAACCAGAACGACCCATCGAAACTTTTTCACTCCAATCTCCGttt                                  >  2:8698112/1‑140 (MQ=255)

                                                                                                        aaTGGCACAAATTCCGCTGAAAAATTGTACATCC‑TGTATCGCACATCATGTTGAATACCTGCGCCCAAAGCAGCAAGCAAGCCAACACCACGACAACCAGAACGACCCATCGAAACTTTTTCACTCCAATCTCCGTTTCa                                >  2:635808/1‑140 (MQ=255)

                                                                                                         aTGGCACAACTTCCGCTGAAAAATTGTACATCC‑TGTATCGCACATCATGTTGAATACCTGCGCCCAAAGCAGCAAGCAAGCCAACACCACGACAACCAGAACGACCCATCGAAACTTTTTCACTCCAATCTCCGTTTCac                               <  1:881255/140‑1 (MQ=255)

                                                                                                         aTGGCACAAATTCCGCTGAAAAATTGTACATCC‑TGTATCGCACATCATGTTGAATACCTGCGCCCAAAGCAGCAAGCAAGCCAACACCACGACAACCAGAACGACCCATCGAAACTTTTTCACTCCAATCTCCGTTTCac                               <  1:2516027/140‑1 (MQ=255)

                                                                                                         aTGGCACAAATTCCGCTGAAAAATTGTACATCC‑TGTATCGCACATCATGTTGAATACCTGCGCCCAAAGCAGCAAGCAAGCCAACACCACGACAACCAGAACGACCCATCGAAACTTTTTCACTCCAATCTCCGTTTCac                               <  1:7861896/140‑1 (MQ=255)

                                                                                                         aTGGCACAAATTCCGCTGAAAAATTGTACATCC‑TGTATCGCACATCATGTTGAATACCTGCGCCCAAAGCAGCAAGCAAGCCAACACCACGACAACCAGAACGACCCATCGAAACTTTTTCACTCCAATCTCCGTTTCac                               <  1:4119134/140‑1 (MQ=255)

                                                                                                          tGGCACAAATTCCGCTGAAAAATTGTACATCC‑TGTATCGCACATCATGTTGAATACCTGCGCCCAAAGCAGCAAGCAAGCCAACACCACGACAACCAGAACGACCCATCGAAACTTTTTCACTCCAATCTCCGTTTCacc                              >  1:71369/1‑140 (MQ=255)

                                                                                                          tGGCACAAATTCCGCTGAAAAATTGTACATCC‑TGTATCGCACATCATGTTGAATACCTGCGCCCAAAGCAGCAAGCAAGCCAACACCACGACAACCAGAACGACCCATCGAAACTTTTTCACTCCAATCTCCGTTTCacc                              <  1:3500209/140‑1 (MQ=255)

                                                                                                               caAATTCCGCTGAAAAATTGTACATCC‑TGTATCGCACATCATGTTGAATACCTGCGCCCAAAGCAGCAAGCAAGCCAACACCACGACAACAATAACGACCCATCGAAACTTTTTCACTCCAATCTCCGTTTCtcctaccg                         >  2:8110142/1‑139 (MQ=255)

                                                                                                                aaaTTCCGCTGAAAAATTGTACATCC‑TGTATCGCACATCATGTTGAATACCTGCGCCCAAAGCAGCAAGCAAGCCAACACCACGACAACCAGAACGACCCATCGAAACTTTTTCACTCCAATCTCCGTTTCACCTACCtt                        <  2:1201185/140‑1 (MQ=255)

                                                                                                                 aaTTCCGCTGAAAAATTGTACATCC‑TGTATCGCACATCATGTTGAATACCTGCGCCCAAAGCAGCAAGCAAGCCAACACCACGACAACCAGAACGACCCATCGAAACTTTTTCACTCCAATCTCCGTTTCACCTACCTTa                       <  2:8344135/140‑1 (MQ=255)

                                                                                                                 aaTTCCGCTGAAAAATTGTACATCC‑TGTATCGCACATCATGTTGAATACCTGCGCCCAAAGCAGCAAGCAAGCCAACACCACGACAACCAGAACGACCCATCGAAACTTTTTCACTCCAATCTCCGTTTCACCTACCTTa                       <  1:7391166/140‑1 (MQ=255)

                                                                                                                  aTTCCGCTGAAAAATTGTACATCC‑TGTATCGCACATCATGTTGAATACCTGCGCCCAAAGCAGCAAGCAAGCCAACACCACGACAACCAGAACGACCCATCGAAACTTTTTCACTCCAATCTCCGTTTCACCTACCTTAt                      <  2:9114483/140‑1 (MQ=255)

                                                                                                                  aTTCCGCTGAAAAATTGTACATCC‑TGTATCGCACATCATGTTGAATACCTGCGCCCAAAGCAGCAAGCAAGCCAACACCACGACAACAAGAACGACACATCGAAACTTTTTCACTCCAATCTCCGTTTCacctacctgag                      >  2:9416748/1‑137 (MQ=255)

                                                                                                                      cGCTGAAAAATTGTACATCC‑TGTATCGCACATCATGTTGAATACCTGCGCCCAAAGCAGCAAGCAAGCCAACACCACGACAACCAGAACGACCCATCGAAACTTTTTCACTCCAATCTCCGTTTCACCTACCTTATGTCa                  <  1:5801469/140‑1 (MQ=255)

                                                                                                                      cGCTGAAAAATTGTACATCC‑TGTATCGCACATCATGTTGAATACCTGCGCCCAAAGCAGCAAGCAAGCCAACACCACGACAACCAGAACGACCCATCGAAACTTTTTCACTCCAATCTCCGTTTCACCTACCTTATGTCa                  <  1:950246/140‑1 (MQ=255)

                                                                                                                      cGCTGAAAAATTGTACATCC‑TGTATCGCACATCATGTTGAATACCTGCGCCCAAAGCAGCAAGCAAGCCAACACCACGACAACCAGAACGACCCATCGAAACTTTTTCACTCCAATCTCCGTTTCACCTACCTTATGTCa                  <  2:3847946/140‑1 (MQ=255)

                                                                                                                                    aCATCC‑TGTATCGCACATCATGTTGAATACCTGCGCCCAAAGCAGCAAGCAAGCCAACACCACGACAACCAGAACGACCCATCGAAACTTTTTCACTCCAATCTCCGTTTCACCTACCTTATGTCATTAAACTAGCATGc    <  1:8554434/140‑1 (MQ=255)

                                                                                                                                    aCATCC‑TGTATCGCACATCATGTTGAATACCTGCGCCCAAAGCAGCAAGCAAGCCAACACCACGACAACCAGAACGACCCATCGAAACTTTTTCACTCCAATCTCCGTTTCACCTACCTTATGTCATTAAACTAGCATGc    <  2:4203207/140‑1 (MQ=255)

                                                                                                                                    aCATCC‑TGTATCGCACATCATGTTGAATACCTGCGCCCAAAGCAGCAAGCAAGCCAACACCACGACAACCAGAACGACCCATCGAAACTTTTTCACTCCAATCTCCGTTTCACCTACCTTATGTCATTAAACTAGCATGc    <  1:1435718/140‑1 (MQ=255)

                                                                                                                                    aCATCC‑TGTATCGCACATCATGTTGAATACCTGCGCCCAAAGCAGCAAGCAAGCCAACACCACGACAACCAGAACGACCCATCGAAACTTTTTCACTCCAATCTCCGTTTCACCTACCTTATGTCATTAAACTAGCATGc    <  2:4911171/140‑1 (MQ=255)

                                                                                                                                      aTCC‑TGTATCGCACATCATGTTGAATACCTGCGCCCAAAGCAGCAAGCAAGCCAACACCACGACAACCAGAACGACCCATCGAAACTTTTTCACTCCAATCTCCGTTTCACCTACCTTATGTCATTAAACTAGCATGCgt  <  1:529327/140‑1 (MQ=255)

                                                                                                                                      aTCC‑TGTATCGCACATCATGTTGAATACCTGCGCCCAAAGCAGCAAGCAAGCCAACACCACGACAACCAGAACGACCCATCGAAACTTTTTCACTCCAATCTCCGTTTCACCTACCTTATGTCATTAAACTAGCATGCgt  <  1:8311673/140‑1 (MQ=255)

                                                                                                                                      aTCC‑TGTATCGCACATCATGTTGAATACCTGCGCCCAAAGCAGCAAGCAAGCCAACACCACGACAACCAGAACGACCCATCGAAACTTTTTCACTCCAATCTCCGTTTCACCTACCTTATGTCATTAAACTAGCATGCgt  <  2:1578301/140‑1 (MQ=255)

TTCGTTCATTTCACCACCTCTGAGTAGAGGCGCTATTCTACCACTGCTGGAGAGGAAGAAAATCTAGTGCTGAAAAAATGATATCACCACGGGATAAACTGGTTAATGGCACAAATTCCGCTGAAAAATTGTACATCC‑TG‑ATCGCACATCATGTTGAATACCTGCGCCCAAAGCAGCAAGCAAGCCAACACCACGACAACCAGAACGACCCATCGAAACTTTTTCACTCCAATCTCCGTTTCACCTACCTTATGTCATTAAACTAGCATGCGT  >  NZ_CP025268/1913353‑1913625

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`