BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP025268	2,204,490	(A)_7→8	pseudogene (175/3632 nt)	CXP41_RS11705 →	DUF4132 domain‑containing protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP025268	2,204,483	1	.	A	96.1%	1637.5 / 45.4	436	pseudogene (168/3632 nt)	CXP41_RS11705	DUF4132 domain‑containing protein
Reads supporting (aligned to +/- strand): ref base . (7/10); new base A (220/197); total (228/208)
Fisher's exact test for biased strand distribution p-value = 4.59e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 467 total aligned reads displayed.

ATAACGCCCAACTGGAACTGAAATATAAAAAAGGCAAAACGCCGCTCAGTCATCGTCGCTGGCCGGGCGAACCAGTGTCCGTTATCACTGGAAGTCTCATCCAGACATTGGGTGATGAATTGCTACAAAAAGCTGAGAA‑G‑AA‑A‑AAA‑ACATTGTCTGGCGTTATGAGAATTTTTCACTGGAGTGGCAGTCCGCCATCACGCAGGCCATCAACTTGATCGGCGAACACAAACCCTCAATCCCGGCCCGGACAATGGCGGCGCTAGCCTGTATCGCG  >  NZ_CP025268/2204344‑2204617

          aCTGGAACTGAAATATAAAAAAGGCAAAACGCCGCTCAGTCATCGTCGCTGGCCGGGCGAACCAGTGTCCGTTATCACTGGAAGTCTCATCCAGACATTGGGTGATGAATTGCTACAAAAAGCTGAGAA‑G‑aa‑a‑aaa‑aaca                                                                                                                              >  2:2222520/1‑137 (MQ=255)

          aCTGGAACTGAAATATAAAAAAGGCAAAACGCCGCTCAGTCATCGTCGCTGGCCGGGCGAACCAGTGTCCGTTATCACTGGAAGTCTCATCCAGACATTGGGTGATGAATTGCTACAAAAAGCTGAGAA‑G‑aa‑a‑aaa‑aaca                                                                                                                              >  1:4422890/1‑137 (MQ=255)

          aCTGGAACTGAAATAGAAAAAAGGCAAAACGCCGCTCAGTCATCGTCGCTGGCCGGGCGAACCAGTGTCCGTTATCACTGGAAGTCTAATCCAGACTTTGGGTGATGAATTGCTACATAAAGCTGAGAA‑G‑aa‑a‑aaa‑aaca                                                                                                                              <  2:8228986/140‑4 (MQ=255)

               aaCTGAAATATAAAAAAGGCAAAACGCCGCTCAGTCATCGTCGCTGGCCGGGCGAACCAGTGTCCGTTATCACTGGAAGTCTCATCCAGACATTGGGTGATGAATTGCTACAAAAAGCTGAGAA‑GAAA‑A‑AAA‑ACATTGTc                                                                                                                          >  2:4164341/1‑140 (MQ=255)

                      atatAAAAAAGGCAAAACGCCGCTCAGTCATCGTCGCTGGCCGGGCGAACCAGTGTCCGTTATCACTGGAAGTCTCATCCAGACATTGGGTGATGAATTGCTCCAAAATGCTGAGAA‑GAAA‑A‑AAA‑ACAGTGTCTGGCGtt                                                                                                                   >  2:5617447/1‑140 (MQ=255)

                        ataAAAAAGGCAAAACGCCGCTCAGTCATCGTCGCTGGCCGGGCGAACCAGTGTCCGTTATCACTGGAAGTCTCATCCAGACATTGGGTGATGAATTGCTACAAAAAGCTGAGAA‑GAAA‑A‑AAA‑ACATTGTCTGGCGTTAt                                                                                                                 >  2:2088957/1‑140 (MQ=255)

                         taAAAAAGGCAAAACGCCGCTCAGTCATCGTCGCTGGCCGGGCGAACCAGTGTCCGTTATCACTGGAAGTCTCATCCAGACATTGGGTGATGAATTGCTACAAAAAGCTGAGAA‑GAAA‑A‑AAA‑ACATTGTCTGGCGTTATg                                                                                                                >  1:8678602/1‑140 (MQ=255)

                         taAAAAAGGCAAAACGCCGCTCAGTCATCGTCGCTGGCCGGGCGAACCAGTGTCCGTTATCACTGGAAGTCTCATCCAGACATTGGGTGATGAATTGCTACAAAAAGCTGAGAA‑GAAA‑A‑AAA‑ACATTGTCTGGCGTTATg                                                                                                                >  1:9102274/1‑140 (MQ=255)

                         taAAAAAGGCAAAACGCCGCTCAGTCATCGTCGCTGGCCGGGCGAACCAGTGTCCGTTATCACTGGAAGTCTCATCCAGACATTGGGTGATGAATTGCTACAAAAAGCTGAGAA‑GAAA‑A‑AAA‑ACATTGTCTGGCGTTATg                                                                                                                >  2:8901171/1‑140 (MQ=255)

                            aaaaGGCAAAACGCAGCTCAGTCATCGTCGCTGGCCGGGCGAACCAGTGTCCGTTATCACTGGAAGTCTCATCCAGACATTGGGTGATGAATTGCTACAAAAAGCTGAGAA‑GAAA‑A‑AAA‑ACATTGTCTGGCGTGATgaga                                                                                                             >  2:6787225/1‑140 (MQ=255)

                                 gCAAAACGCCGCTCAGTCATCGTCGCTGGCCGGGCGAACCAGTGTCCGTTATCACTGGAAGTCTCATCCAGACATTGGGTGATGAATTGCTACAAAAAGCTGTGAC‑G‑AACA‑AAA‑ACATTGTCTGGCGTTATGGGACtttt                                                                                                        >  1:1290488/1‑140 (MQ=255)

                                   aacaCGCCGCTCAGTCATCGTCGCTGGCCGGGCGAACCAGTGTCCTTTATCACTGGAAGTCTCATCCAGACATTGGGTGATGAATTGCTACAAAAAGCTGAGAA‑GAAA‑A‑AAA‑ACATTGTCTGGCGTTATGAGAATTTTTc                                                                                                      <  2:7826121/137‑1 (MQ=255)

                                   aaaaCGCCGCTCAGTCATCGTCGCTGGCCGGGCGAACCAGTGTCCGTTATCACTGGAAGTCTCATCCAGACATTGGGTGATGAATTGCTACAAAAAGCTGAGAA‑GAAA‑A‑AAA‑ACATTGTCTGGCGTTATGAGAATTTTTc                                                                                                      <  1:6410802/140‑1 (MQ=255)

                                   aaaaCGCCGCTCAGTCATCGTCGCTGGCCGGGCGAACCAGTGTCCGTTATCACTGGAAGTCTCATCCAGACATTGGGTGATGAATTGCTACAAAAAGCTGAGAA‑GAAA‑A‑AAA‑ACATTGTCTGGCGTTATGAGAATTTTTc                                                                                                      <  1:1999316/140‑1 (MQ=255)

                                   aaaaCGCCGCTCAGTCATCGTCGCTGGCCGGGCGAACCAGTGTCCGTTATCACTGGAAGTCTCATCCAGACATTGGGTGATGAATTGCTACAAAAAGCTGAGAA‑GAAA‑A‑AAA‑ACAGTGTCTGGCGTTATGAGAATTTTTc                                                                                                      <  2:860457/140‑1 (MQ=255)

                                       cgccgcTCAGTCATCGTCGCTGGCCGGGCGAACCAGTGTCCGTTATCACTGGAAGTCTCATCCAGACATTGGGTGATGAATTGCTACAAAAAGCTGAGAA‑GAAA‑A‑AAA‑ACATTGTCTGGCGTTATGAGAATTTTTCACTg                                                                                                  <  1:8818670/140‑1 (MQ=255)

                                       cgccgcTCAGTCATCGTCGCTGGCCGGGCGAACCAGTGTCCGTTATCACTGGAAGTCTCATCCAGACATTGGGTGATGAATTGCTACAAAAAGCTGAGAA‑GAAA‑A‑AAA‑ACATTGTCTGGCGTTATGAGAATTTTTCACTg                                                                                                  <  2:2742470/140‑1 (MQ=255)

                                            cTCAGTCATCGTCGCTGGCCGGGCGAACCAGTGTCCGTTATCACTGGAAGTCTCATCCAGACATTGGGTGATGAATTGCTACAAAAAGCTGAGAA‑GAAA‑A‑AAA‑ACATTGTCTGGCGTTATGAGAATTTTTCACTGGAGTg                                                                                             >  2:3968462/1‑140 (MQ=255)

                                                   aTCGTCGCTGGCCGGGCGAACCAGTGNCCGTTATCACTGGAAGTCTCATCCAGACATTGGGTGATGAATTGCTACAAAAAGCTGAGAA‑GAAA‑A‑AAA‑ACATTGTCTGGCGTTATGAGAATTTTTCACNGGAGTGGCAGTcc                                                                                      <  1:6227445/140‑1 (MQ=255)

                                                      gtcgCTGGCCGGGCGAACCAGTGTCCGTTATCACTGGAAGTCTCATCCAGACATTGGGTGATGAATTGCTACAAAAAGCTGAGAA‑GAAA‑A‑AAA‑ACATTGTCTGGCGTTATGAGAATTTTTCACTGGAGTGGCAGTCCGcc                                                                                   <  2:9157475/140‑1 (MQ=255)

                                                           tGGCCGGGCGAACCAGTGTCCGTTATCACTGGAAGTCTCATCCAGACATTGGGTGATGAATTGCTACAAAAAGCTGAGAA‑GAAA‑A‑AAA‑ACATTGTCTGGCGTTATGAGAATTTTTCACTGGAGTGGCAGTCCGCCATCAc                                                                              >  1:4495508/1‑140 (MQ=255)

                                                           tGGCCGGGCGAACCAGTGTCCGTTATCACTGGAAGTCTCATCCAGACATTGGGTGATGAATTGCTACAAAAAGCTGAGAA‑GAAA‑A‑AAA‑ACATTGTCTGGCGTTATGAGAATTTTTCACTGGAGTGGCAGTCCGCCATCAc                                                                              >  1:4744480/1‑140 (MQ=255)

                                                           tGGCCGGGCGAACCAGTGTCCGTTATCACTGGAAGTCTCATCCAGACATTGGGTGATGAATTGCTACAAAAAGCTGAGAA‑GAAA‑A‑AAA‑ACATTGTCTGGCGTTATGAGAATTTTTCACTGGAGTGGCAGTCCGCCATCAc                                                                              >  1:3070707/1‑140 (MQ=255)

                                                             gCCGGGCGAACCAGTGTCCGTTATCACTGGAAGTCTCATCCAGACATTGGGTGATGAATTGCTACAAAAAGCTGAGAA‑GAAA‑A‑AAA‑ACATTGTCTGGCGTTATGAGAATTTTTCACTGGAGTGGCAGTCCGCCATCACGc                                                                            <  2:3590363/140‑1 (MQ=255)

                                                             gCCGGGCGAACCAGTGTCCGTTATCACTGGAAGTCTCATCCAGACATTGGGTGATGAATTGCTACAAAAAGCTGAGAA‑GAAA‑A‑AAA‑ACATGGTCTGGCGTTATGAGAATTTTTCACTGGAGTGGCAGTCCGCCATCACGc                                                                            <  2:2869122/140‑1 (MQ=255)

                                                              ccGGGCGAACCAGTGTCCGTTATCACTGGAAGTCTCATCCAGACATTGGGTGATGAATTGCTACAAAAAGCTGAGAA‑GAAA‑A‑AAA‑ACATTGTCTGGCGTTATGAGAATTTTTCACTGGAGTGGCAGTCCGCCATCACGCa                                                                           <  2:6456797/140‑1 (MQ=255)

                                                              ccGGGCGAACCAGTGTCCGTTATCACTGGAAGTCTCATCCAGACATTGGGTGATGAATTGCTACAAAAAGCTGAGAA‑GAAA‑A‑AAA‑ACATTGTCTGGCGTTATGAGAATTTTTCACTGGAGTGGCAGTCCGCCATCACGCa                                                                           >  1:3241163/1‑140 (MQ=255)

                                                               cGGGCGAACCAGTGTCCGTTATCACTGGAAGTCTCATCCAGACATTGGGTGATGAATTGCTACAAAAAGCTGAGAA‑GAAA‑A‑AAA‑ACATTGTCTGGCGTTATGAGAATTTTTCACTGGAGTGGCAGTCCGCCATCACGCAg                                                                          >  1:27661/1‑140 (MQ=255)

                                                                 ggCGAACCAGTGTCCGTTATCACTGGAAGTCTCATCCAGACATTGGGTGATGAATTGCTACAAAAAGCTGAGAA‑GAAA‑A‑AAA‑ACATTGTCTGGCGTTATGAGAATTTTTCACTGGAGTGGCAGTCCGCCATCACGCAGGc                                                                        >  2:1294695/1‑140 (MQ=255)

                                                                  gCGAACCAGTGTCCGTTATCACTGGAAGTCTCATCCAGACATTGGGTGATGAATTGCTACAACAAGCTGAGAA‑GAAA‑A‑AAA‑ACATGGGCTGGCGTTATGAGAAGTTTTCACTGGAGTGGCAGTCCGCCATCACGCAGGcc                                                                       <  1:2543991/140‑1 (MQ=255)

                                                                  gCGAACCAGTGTCCGTTATCACTGGAAGTCTCATCCAGACATTGGGTGATGAATTGCTACAAAAAGCTGAGAA‑GAAA‑A‑AAA‑ACATTGTCTGGCGTTATGAGAATTTTTCACTGGAGTGGCAGTCCGCCATCACGCAGGcc                                                                       <  2:3462487/140‑1 (MQ=255)

                                                                  gCGAACCAGTGTCCGTTATCACTGGAAGTCTCATCCAGACATTGGGTGATGAATTGCTACAAAAAGCTGAGAA‑GAAA‑A‑AAA‑ACATTGTCTGGCGTTATGAGAATTTTTCACTGGAGTGGCAGTCCGCCATCACGCAGGcc                                                                       >  1:6978986/1‑140 (MQ=255)

                                                                  gCGAACCAGTGTCCGTTATCACTGGAAGTCTCATCCAGACATTGGGTGATGAATTGCTACAAAAAGCTGAGAA‑GAAA‑A‑AAA‑ACATTGTCTGGCGTTATGAGAATTTTTCACTGGAGTGGCAGTCCGCCATCACGCAGGcc                                                                       >  2:6118569/1‑140 (MQ=255)

                                                                   cGAACCAGTGTCCGTTATCACTGGAAGTCTCATCCAGACATTGGGTGATGAATTGCTACAAAAAGCTGAGAA‑GAAA‑A‑AAA‑ACATTGTCTGGCGTTATGAGAATTTTTCACTGGAGTGGCAGTCCGCCATCACGCAGGCCa                                                                      <  1:1627467/140‑1 (MQ=255)

                                                                   cGAACCAGTGTCCGTTATCACAGGAAGTCTCATCCAGACATTGGGTGATGAATTGCTACAAAAAGCTGAGAA‑GAAA‑A‑AAA‑ACATTGTCTGGCGTTATGAGAATTTTTCACTGGAGTGGCAGTCCGCCATCACGCAGGCCa                                                                      <  2:327373/140‑1 (MQ=255)

                                                                      aCCAGTGTCCGTTATCACTGGAAGTCTCATCCAGACATTGGGTGATGAATTGCTACAAAAAGCTGAGAA‑GAAA‑A‑AAA‑ACATTGTCTGGCGTTATGAGAATTTTTCACTGGAGTGGCAGTCCGcc                                                                                   >  1:3986189/1‑124 (MQ=255)

                                                                           tgtCCGTTATCACTGGAAGTCTCATCCAGACATTGGGTGATGAATTGCTACAAAAAGCTGAGAA‑GAAA‑A‑AAA‑ACATTGTCTTGCGTTATGAGAATTTTTCACTGGAGTGGCAGTCCGCCATCACGCAGGCCATCAACTTg                                                              >  1:1081721/1‑140 (MQ=255)

                                                                            gtCCGTTATCACTGGAAGTCTCATCCAGACATTGGGTGATGAATTGCTACAAAAAGCTGAGAA‑GAAA‑A‑AAA‑ACATTGTCTGGCGTTATGAGAATTTTTCACTGGAGTGGCAGTCCGCCATCACGCAGGCCATCAACTTGa                                                             <  2:8335563/140‑1 (MQ=255)

                                                                            gtCCGTTATCACTGGAAGTCTCATCCAGACATTGGGTGATGAATTGCTACAAAAAGCTGAGAA‑GAAA‑A‑AAA‑ACATTGTCTGGCGTTATGAGAATTTTTCACTGGAGTGGCAGTCCGCCATCACGCAGGCCATCAACTTGa                                                             <  2:7277569/140‑1 (MQ=255)

                                                                            gtCCGTTATCACTGGAAGTCTCATCCAGACATTGGGTGATGAATTGCTACAAAAAGCTGAGAA‑GAAA‑A‑AAA‑ACATTGTCTGGCGTTATGAGAATTTTTCACTGGAGTGGCAGTCCGCCATCACGCAGGCCATCAACTTGa                                                             >  2:1959781/1‑140 (MQ=255)

                                                                                gTTATCACTGGAAGTCTCATCCAGACATTGGGTGATGAATTGCTACAAAAAGCTGAGAA‑GAAA‑A‑AAA‑ACATTGTCTGGCGTTATGAGAATTTTTCACTGGAGTGGCAGTCCGCCATCACGCAGGCCATCAACTTGAt                                                            <  1:5570498/137‑1 (MQ=255)

                                                                                gTTATCACTGGAAGTCTCATCCAGACATTGGGTGATGAATTGCTACAAAAAGCTGAGAA‑GAAA‑A‑AAA‑ACATTGTCTGGCGTTATGAGAATTTTTCACTGGAGTGGCAGTCCGCCATCACGCAGGCCATCAACTTGAt                                                            >  2:5570498/1‑137 (MQ=255)

                                                                                gTTATCACTGGAAGTCTCATCCAGACATTGGGTGATGAATTGCTACAAAAAGCTGAGAA‑GAAA‑A‑AAA‑ACATTGTCTGGCGTTATGAGAATTTTTCACTGGAGTGGCAGTCCGCCATCACGCAGGCCATCAACTTGATCgg                                                         >  2:9322537/1‑140 (MQ=255)

                                                                                  tATCACTGGAAGTCTCATCCAGACATTGGGTGATGAATTGCTACAAAAAGCTGAGAA‑GAAA‑A‑AAA‑ACATTGTCTGGCGTTATGAGAATTTTTCACTGGAGTGGCAGTCCGCCATCACGCAGGCCATCAACTTGATCGGCg                                                       >  2:1145568/1‑140 (MQ=255)

                                                                                     cACTGGAAGTCTCATCCAGACATTGGGTGATGAATTGCTACAAAAAGCTGAGAA‑GAAA‑A‑AAA‑ACATTGTCTGGCGTTATGAGAATTTTTCACTGGAGTGGCAGTCCGCCATCACGCAGGCCATCAACTTGATCGGCGAac                                                    >  2:733647/1‑140 (MQ=255)

                                                                                     cACTGGAAGTCTCATCCAGACATTGGGTGATGAATTGCTACAAAAAGCTGAGAA‑GAAA‑A‑AAA‑ACATTGTCTGGCGTTATGAGAATTTTTCACTGGAGTGGCAGTCCGCCATCACGCAGGCCATCAACTTGATCGGCGAac                                                    >  1:4055991/1‑140 (MQ=255)

                                                                                       cTGGAAGTCTCATCCCGACATTGGGTGATGAATTGCTACATAAAGCTGAGAG‑G‑AA‑A‑AAATACATTGTCTGGCGTGTGGAGAATTTGTCACTGGAGTGGCAGGCCGCGCTCACGCAGGCCATCATCTTGATCGGCGAacac                                                  >  2:6307533/1‑140 (MQ=255)

                                                                                        tGGACGTCTCATCCAGACATTGGGTGATGAATTGCTACAAAAAGCTGAGAA‑GAAA‑A‑AAA‑ACAGTGTCTGGCGTTATGAGAATTTTTCACTGGAGTGGCAGTCCGCCATCACGCAGGCCATCAACTTGATCGGCGAAcaca                                                 <  2:4534163/140‑1 (MQ=255)

                                                                                        tGGACGTCTCATCCAGACATTGGGTGATGAATTGCTACAAAAAGCTGAGAA‑G‑AA‑ACAAA‑ACAGTGTCTGGCGTTATGAGAATTTTTCACTGGAGTGGCAGTCCGCCATCACGCAGGCCATCAACTTGATCGGCGAAcaca                                                 <  1:4947489/140‑1 (MQ=255)

                                                                                         ggAAGTCTCATCCAGACATTGGGTGATGAATTGCTACAAAAAGCTGAGAA‑GAAA‑A‑AAA‑ACATTGTCTGGCGTTATGAGAATTTTTCACTGGAGTGGCAGTCCGCCATCACGCAGGCCATCAACTTGATCGGCGAACACaa                                                <  1:7442340/140‑1 (MQ=255)

                                                                                            aGTCTCATCCAGACATTGGGTGATTAATTGATACAAAAAGCTGAGAA‑GAAA‑A‑AAA‑ACATTGTCTGGCGTTATGAGAATTTTTCACTGGAGTGGCAGTCCGCCATCACGCAGGCCATCAACTTGATCGGCGAACACATAcc                                             <  2:1669072/140‑1 (MQ=255)

                                                                                            aGTCTCATCCAGACATTGGGTGATGAATTGCTACAAAAAGCTGAGAA‑GAAA‑A‑AAA‑ACATTGTCTGGCGTTATGAGAATTTTTCACTGGAGTGGCAGTCCGCCATCACGCAGGCCATCAACTTGATCGGCGAACACAAAca                                             >  2:6651438/1‑139 (MQ=255)

                                                                                              tctcATCCAGACATTGGGTGATGAATTGCTACAAACAGCTGAGAA‑GAAA‑A‑AAA‑ACATTGTCTGGCGTTATGAGAATTTTTCACTGGAGTGGCAGTCCGCCATCACGCAGGCCATCTACTTGATCGGCGAACACAAACCCt                                           <  1:4247892/140‑1 (MQ=255)

                                                                                              tctcATCCAGACATTGGGTGATGAATTGCTACAAAAAGCTGAGAA‑GAAA‑A‑AAA‑ACATTGTCTGGCGTTATGAGAATTTTTCACTGGAGTGGCAGTCCGCCATCACGCAGGCCATCAACTTGATCGGCGAACACAAACCCt                                           <  2:4679595/140‑1 (MQ=255)

                                                                                              tctcATCCAGACATTGGGTGATGAATTGCTACAAAAAGCTGAGAA‑GAAA‑A‑AAA‑ACATTGTCTGGCGTTATGAGAATTTTTCACTGGAGTGGCAGTCCGCCATCACGCAGGCCATCAACTTGATCGGCGAACACAAACCCt                                           >  1:7785984/1‑140 (MQ=255)

                                                                                              tctcATCCAGACATTGGGTGATGAATTGCTACAAAAAGCTGAGAA‑GAAA‑A‑AAA‑ACATTGTCTGGCGTTATGAGAATTTTTCACTGGAGTGGCAGTCCGCCATCACGCAGGCCATCAACTTGATCGGCGAACACAAACCCt                                           <  1:7890897/140‑1 (MQ=255)

                                                                                               ctcATCCAGACATTGGGTGATGAATTGCTACAAAAAGCTGAGAA‑GAAA‑A‑AAA‑ACATTGTCTGGCGTTATGAGAATTTTTCACTGGAGTGGCAGTCCGCCATCACGCAGGCCATCAACTTGATCGGCGAACACAAACCCTc                                          >  2:6535904/1‑140 (MQ=255)

                                                                                                  aTCCAGACATTGGGTGATGAATTGCTACAAAAAGCTGAGAA‑GAAA‑A‑AAA‑ACATTGTCTGGCGTTATGAGAATTTTTCACTGGAGTGGCAGTCCGCCATCACGCAGGCCATCAACTTGATCGGCGAACACAAACCCTCAAt                                       >  2:547408/1‑140 (MQ=255)

                                                                                                  aTCCAGACATTGGGTGATGAATTGCTACAAAAAGCTGAGAA‑GAAA‑A‑AAA‑ACATTGTCTGGCGTTATGAGAATTTTTCACTGGAGTGGCAGTCCGCCATCACGCAGGCCATCAACTTGATCGGCGAACACAAACCCTCAAt                                       >  2:4926897/1‑140 (MQ=255)

                                                                                                   tCCAGACATTGGGTGATGAATTGCTACACAAAGCTGAGAA‑GAAA‑A‑AAA‑ACAGTGTCTGGCGTTATGAGAAGTTTTCACTGGAGTGGCAGTCCGCCATCACGCAGGCCATCACCTTGCTCGGCGAACACAAACCCTCACTc                                      <  2:4379557/140‑1 (MQ=255)

                                                                                                   tCCAGACATTGGGTGATGAATTGCTACAAAAAGCTGAGAA‑GAAA‑A‑AAA‑ACATTGTCTGGCGTTATGAGAATTTTTCACTGGAGTGGCAGTCCGCCATCACGCAGGCCATCAACTTGATCGGCGAACACAAACCCTCAATc                                      <  1:963244/140‑1 (MQ=255)

                                                                                                   tCCAGACATTGGGTGATGAATTGCTACAAAAAGCTGAGAA‑GAAA‑A‑AAA‑ACATTGTCTGGCGTTATGAGAATTTTTCACTGGAGTGGCAGTCCGCCATCACGCAGGCCATCAACTTGATCGGCGAACACAAACCCTCAATc                                      <  2:5855151/140‑1 (MQ=255)

                                                                                                   tCCAGACATTGGGTGATGAATTGCTACAAAAAGCTGAGAA‑GAAA‑A‑AAA‑ACATTGTCTGGCGTTATGAGAATTTTTCACTGGAGTGGCAGTCCGCCATCACGCAGGCCATCAACTTGATCGGCGAACACAAACCCTCAATc                                      <  2:6931168/140‑1 (MQ=255)

                                                                                                      aGACATTGGGTGATGAATTGCTACAAAAAGCTGAGAA‑GAAA‑A‑AAA‑ACATTGTCTGGCGTTATGAGAATTTTTCACTGGAGTGGCAGTCCGCCATCACGCAGGCCATCAACTTGATCGGCGAACACAAACCCTCAATcccg                                   <  1:8932335/140‑1 (MQ=255)

                                                                                                        aCATTGGGTGATGAATTGCTACAAAAAGCTGAGAA‑GAAA‑A‑AAA‑ACATTGTCTGGCGTTATGAGAATTTTTCACTGGAGTGGCAGTCCGCCATCACGCAGGCCATCAACTTGATCGGCGAACACAAACCCTCAATcccggc                                 <  1:3683307/140‑1 (MQ=255)

                                                                                                        aCATTGGGTGATGAATTGCTACAAAAAGCTGAGAA‑GAAA‑A‑AAA‑ACATTGTCTGGCGTTATGAGAATTTTTCACTGGAGTGGCAGTCCGCCATCACGCAGGCCATCAACTTGATCGGCGAACACAAACCCTCAATcccggc                                 >  1:524416/1‑140 (MQ=255)

                                                                                                        aCATTGGGTGATGAATGGCTACAAAAAGCTGAGAA‑GAAA‑A‑AAA‑ACCTTGTCTGGCGTTATGAGAATTTTTCACTGGAGTGGCAGTCCGCCATCACGCAGGCCATCAACTTGATCGGCTAACACAAACCCTCAATcccggc                                 <  2:4340347/140‑1 (MQ=255)

                                                                                                        aCAGTGGGTGATGAATTGCTACAAAAAGCTGAGAA‑GAAA‑A‑AAA‑ACATTGTCTGGCGTTATGAGAATTTTTCTCTGGAGTGGCAGTCCGCCATCACGCAGGCCATCAACTTGATCGGCGAACACAAACCCTCAATcccggc                                 >  2:752956/1‑140 (MQ=255)

                                                                                                          aTTGGGTGATGAATTGCTACAAAAAGCTGAGAA‑GAAA‑A‑AAA‑ACATTGTCTGGCGTTATGAGAATTTTTCACTGGAGTGGCAGTCCGCCATCACGCAGGCCATCAACTTGATCGGCGAACACAAACCCTCAATcccggccc                               <  1:6047887/140‑1 (MQ=255)

                                                                                                          aTTGGGTGATGAATTGCTACAAAAAGCTGAGAA‑GAAA‑A‑AAA‑ACATTGTCTGGCGTTATGAGAATTTTTCACTGGAGTGGCAGTCCGCCATCACGCAGGCCATCAACTTGATCGGCGAACACAAACCCTCAATcccggccc                               >  1:5897146/1‑140 (MQ=255)

                                                                                                             gggTGATGAATTGCTACAAAAAGCTGAGAA‑GAAA‑A‑AAA‑ACATTGTCTGGCGTTATGAGAATTTTTCACTGGAGTGGCAGTCCGCCATCACGCAGGCCATCAACTTGATCGGCGAACACAAACCCTCAATCCCGGCCCGGa                            >  1:4580439/1‑140 (MQ=255)

                                                                                                              ggTGATGAATTGCTACAAAAAGCTGAGAA‑GAAA‑A‑AAA‑ACATTGTCTGGCGTTATGAGAATTTTTCACTGGAGTGGCAGTCCGcc                                                                                   <  1:9015095/84‑1 (MQ=255)

                                                                                                              ggTGATGAATTGCTACAAAAAGCTGAGAA‑GAAA‑A‑AAA‑ACATTGTCTGGCGTTATGAGAATTTTTCACTGGAGTGGCAGTCCGCCATCACGCAGGCCATCAACTTGATCGGCGAACACAAACCCTCAATCCCGGCCCGGAc                           >  2:4269053/1‑140 (MQ=255)

                                                                                                              ggTGATGAATTGCTACAAAAAGCTGAGAA‑GAAA‑A‑AAA‑ACATTGTCTGGCGTTATGAGAATTTTTCACTGGAGTGGCAGTCCGCCATCACGCAGGCCATCAACTTGATCGGCGAACACAAACCCTCAATCCCGGCCCGGAc                           >  1:827732/1‑140 (MQ=255)

                                                                                                                  atgaATTGCTACAAAAAGCTGAGAA‑GAAA‑A‑AAA‑ACATTGTCTGGCGTTATGAGAATTTTTCACTGGAGTGGCAGTCCGCCATCACGCAGGCCATCAACTTGATCGGCGAACACNAACCCTCAATCCCGGCCCGGACAATg                       >  1:7116381/1‑140 (MQ=255)

                                                                                                                  atgaATTGCTACAAAAAGCTGAGAA‑GAAA‑A‑AAA‑ACATTGTCTGGCGTTATGAGAATTTTTCACTGGAGTGGCAGTCCGCCATCACGCAGGCCATCAACTTGATCGGCGAACACAAACCCTCAATCCCGGCCCGGACAATg                       >  2:3753588/1‑140 (MQ=255)

                                                                                                                  atgaATTGCTACAAAAAGCTGAGAA‑GAAA‑A‑AAA‑AAAGTGTCTGGCGTTATGAGAATTTTTCACTGGAGTGGCAGTCCGCCATCACGCAGGCCATCAACTTGATCGGCGAACACAAACCCTCAATCCCGGCCCGGACAATg                       >  2:2445826/1‑140 (MQ=255)

                                                                                                                   tgaATTGCTACAAAAAGCTGAGAA‑GAAA‑A‑AAA‑ACATTGTCTGGCGTTATGAGAATTTTTCACTGGAGTGGCAGTCCGCCATCACGCAGGCCATCAACTTGATCGGCGAACACAAACCCTCAATCCCGGCCCGGACAATgg                      <  2:5372945/140‑1 (MQ=255)

                                                                                                                     aaTTGCTACAAAAAGCTGAGAA‑GAAA‑A‑AAA‑ACATTGTCTGGCGTTATGAGAATTTTTCACTGGAGTGGCAGTCCGCCATCACGCAGGCCATCAACTTGATCGGCGAACACAAACCCTCAATCCCGGCCCGGACAATggcg                    >  1:5116023/1‑140 (MQ=255)

                                                                                                                        tGCTACAAACAGCTGAGAA‑GAAA‑A‑AAA‑ACATGGTCTGGCGTTATGAGAATTTTTCACTGGAGTGGCAGTCCGCCATCACGCAGGCCATCAACTTGATCGGCGAACACAAACCCTCAATCCCGGCCCGGACAATGgcggcg                 <  1:2355733/140‑1 (MQ=255)

                                                                                                                        tGCTACAAAAAGCTGAGAACA‑AA‑A‑AAA‑ACATTGTCTGGCGTTATGAGAATTTTTCACTGGAGTGGCAGTCCGCCATCACGCAGGCCATCAACTTGATCGGCGAACACAAACCCTCAATCCCGGCCCGGACAATGgcggcg                 <  1:6567135/140‑1 (MQ=255)

                                                                                                                        tGCTACAAAAAGCTGAGAA‑GAAA‑A‑AAA‑ACATTGTCTGGCGTTATGAGAATTTTTCACTGGAGTGGCAGTCCGCCATCACGCAGGCCATCAACTTGATCGGCGAACACAAACCCTCAATCCCGGCCCGGACAATGgcggcg                 <  1:1550861/140‑1 (MQ=255)

                                                                                                                        tGCTACAAAAAGCTGAGAA‑GAAA‑A‑AAA‑ACATTGTCTGGCGTTATGAGAATTTTTCACTGGAGTGGCAGTCCGCCATCACGCAGGCCATCAACTTGATCGGCGAACACAAACCCTCAATCCCGGCCCGGACAATGgcggcg                 <  1:8528433/140‑1 (MQ=255)

                                                                                                                        tGCTACAAAAAGCTGAGAA‑GAAA‑A‑AAA‑ACATTGTCTGGCGTTATGAGAATTTTTCACTGGAGTGGCAGTCCGCCATCACGCAGGCCATCAACTTGATCGGCGAACACAAACCCTCAATCCCGGCCCGGACAATGgcggcg                 <  1:2222520/140‑1 (MQ=255)

                                                                                                                        tGCTACAAAAAGCTGAGAA‑GAAA‑A‑AAA‑ACATTGTCTGGCGTTATGAGAATTTTTCACTGGAGTGGCAGTCCGCCATCACGCAGGCCATCAACTTGATCGGCGAACACAAACCCTCAATCCCGGCCCGGACAATGgcggcg                 >  1:1892350/1‑140 (MQ=255)

                                                                                                                        tGCTACAAAAAGCTGAGAA‑GAAA‑A‑AAA‑ACATTGTCTGGCGTTATGAGAATTTTTCACTGGAGTGGCAGTCCGCCATCACGCAGGCCATCAACTTGATCGGCGAACACAAACCCTCAATCCCGGCCCGGACAATGgcggcg                 <  1:6772697/140‑1 (MQ=255)

                                                                                                                        tGCTACAAAAAGCTGAGAA‑GAAA‑A‑AAA‑ACATTGTCTGGCGTTATGAGAATTTTTCACTGGAGTGGCAGTCCGCCATCACGCAGGCCATCAACTTGATCGGCGAACACAAACCCTCAATCCCGGCCCGGACAATGgcggcg                 <  2:2646258/140‑1 (MQ=255)

                                                                                                                        tGCTACAAAAAGCTGAGAA‑GAAA‑A‑AAA‑ACATTGTCTGGCGTTATGAGAATTTTTCACTGGAGTGGCAGTCCGCCATCACGCAGGCCATCAACTTGATCGGCGAACACAAACCCTCAATCCCGGCCCGGACAATGgcggcg                 <  1:1004928/140‑1 (MQ=255)

                                                                                                                        tGCTACAAAAAGCTGAGAA‑GAAA‑A‑AAA‑ACATTGTCTGGCGTTATGAGAATTTTTCACTGGAGTGGCAGTCCGCCATCACGCAGGCCATCAACTTGATCGGCGAACACAAACCCTCAATCCCGGCCCGGACAATGgcggcg                 <  1:540169/140‑1 (MQ=255)

                                                                                                                        tGCTACAAAAAGCTGAGAA‑GAAA‑A‑AAA‑ACAGTGTCTGGCGTTATGAGAATTTTTCACTGGAGTGGCAGTCCGCCATCACGCAGGCCATCAACTTGATCGGCGAACACAAACCCTCAATCCCGGCCCGGACAATGgcggcg                 <  1:8120970/140‑1 (MQ=255)

                                                                                                                        tGCTACAAAAAGCTGAGAA‑G‑AA‑A‑AAACACAGTGTCTGGCGTTATGAGAATTTTTCACTGGAGTGGCAGTCCGCCATCACGCAGGCCATCAACTTGATCGGCGAACACAAACCCTCAATCCCGGCCCGGACAATGgcggcg                 <  1:9183149/140‑1 (MQ=255)

                                                                                                                        ggCTACAAACACCTGAGAC‑GAAA‑A‑AAA‑ACATTGTCTGGCGTTATGAGAATTTTTCACTGGAGTGGCAGTCCGCCATCACGCAGGCCATCAACTTGATCGGCGAACACAAACCCACAATCCCGGCCCGGACAATGgcggcg                 <  2:1596801/139‑1 (MQ=255)

                                                                                                                          ctccAAAAAGCTGAGAA‑GAAA‑A‑AAA‑ACATTGTCTGGCGTTATGAGAATTTTTCACTGGAGTGGCAGTCCGCCATCACGCAGGCCATCCCCGTGATCGGCGAGCACAAACCCTCAATCCCGGCCAGGTCCATGGCGGCGCt               >  2:8719948/4‑140 (MQ=255)

                                                                                                                          cTACAAAAAGCTGAGAA‑GAAA‑A‑AAA‑ACATTGTCTGGCGTTATGAGAATTTTTCACTGGAGTGGCAGTCCGCCATCACGCAGGCCATCAACTTGATCGGCGAACACAAACCCTCAATCCCGGCCCGGACAATGGCGGCGCt               >  2:9260644/1‑140 (MQ=255)

                                                                                                                            aCAAAAAGCTGAGAA‑GAAA‑A‑AAA‑ACATTGTCTGGCGTTATGAGAATTTTTCACTGGAGTGGCAGTCCGCCATCACGCAGGCCATCAACTTGATCGGCGAACACAAACCCTCAATCCCGGCCCGGACAATGGCGGCGCTAg             >  1:1397239/1‑140 (MQ=255)

                                                                                                                             cAAAAAGCTTATAA‑GAAA‑A‑AAA‑ACATTGACTGGCGTTATTAGAATTATTCACTGGAGTGGCAGTCCGCCATCACGCACGCCAGCAACATGATCGGCGAACACAAAACCTCAATCCCCGCCCGGACAATGGCGGCGCTAGc            >  2:7124319/1‑140 (MQ=255)

                                                                                                                               aaaaGCTGAGAA‑GAAA‑A‑AAA‑ACATTGTCTGGCGTTATGAGAATTTTTCACTGGAGTGGCAGTCCGCCATCACGCAGGCCATCAACTTGATCGGCGAACACAAACCCTCAATCCCGGCCCGGACAATGGCGGCGCTAGCCt          >  1:9312860/1‑140 (MQ=255)

                                                                                                                               aaaaGCTGAGAA‑GAAA‑A‑AAA‑ACATTGTCTGGCGTTATGAGAATTTTTCACTGGAGTGGCAGTCCGCCATCACGCAGGCCATCAACTTGATCGGCGAACACAAACCCTCAATCCCGGCCCGGACAATGGCGGCGCTAGCCt          >  2:3150211/1‑140 (MQ=255)

                                                                                                                                    cTGAGAA‑GAAA‑A‑AAA‑ACATTGTCTGGCGTTATGAGAATTTTTCACTGGAGTGGCAGTCCGCCATCACGCAGGCCATCAACTTGATCGGCGAACACAAACCCTCAATCCCGGCCCGGACAATGGCGGCGCTAGCCTGTATc     >  2:9028572/1‑140 (MQ=255)

                                                                                                                                       agaa‑gaAA‑A‑AAA‑ACCTTGTCTGGCGTTATGAGAATTTTTCACTGGAGTGGCAGTCCGCCATCACGCAGGCCATCAACTTGATCGGCGAACACGAACCCTCAATCCCGGCCCGGACAATGGCGGCGCTAGCCTGTATcgcg  <  2:5102218/140‑1 (MQ=255)

ATAACGCCCAACTGGAACTGAAATATAAAAAAGGCAAAACGCCGCTCAGTCATCGTCGCTGGCCGGGCGAACCAGTGTCCGTTATCACTGGAAGTCTCATCCAGACATTGGGTGATGAATTGCTACAAAAAGCTGAGAA‑G‑AA‑A‑AAA‑ACATTGTCTGGCGTTATGAGAATTTTTCACTGGAGTGGCAGTCCGCCATCACGCAGGCCATCAACTTGATCGGCGAACACAAACCCTCAATCCCGGCCCGGACAATGGCGGCGCTAGCCTGTATCGCG  >  NZ_CP025268/2204344‑2204617

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`