BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP025268	2,292,596	(C)_6→7	pseudogene (361/2531 nt)	yejO ←	autotransporter YejO

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP025268	2,292,590	1	.	C	97.4%	1535.3 / 29.5	455	pseudogene (367/2531 nt)	yejO	autotransporter YejO
Reads supporting (aligned to +/- strand): ref base . (3/9); new base C (176/265); total (180/275)
Fisher's exact test for biased strand distribution p-value = 3.79e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 483 total aligned reads displayed.

ACCGAGACGGTTTGTTCCGAATACCTCCATGGCCCGCGTGGTTGTTTTAATAGCACCGCCTGCTTTCTGATCTACCGCAAATGCAAATCCCCCCTCTTTAACCTCCAGTAGTCCTCCCTTTTCGATAGCGACAAAGACG‑G‑CCCC‑CCATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCTGCGCCATAAACCTGTAGCACACCATTATTGAGCGTAGTCTGGTTTGCCGCGCCACCCATTTGAACAATTTGATAACCG  >  NZ_CP025268/2292451‑2292723

aCCGAGACGGTTTGTTCCGAATACCTCCATGGCCCGCGTGGTTGTTTTAATAGCACCGCCTGCTTTCTGATCTACCGCAAATGCAAATCCCCCCTCTTTAACCTCCAGTAGTCCTCCCTTTTCGATAGCGACAAAGACg‑g                                                                                                                                         >  2:5212393/1‑140 (MQ=255)

aCCGAGACGGTTTGTTCCGAATACCTCCATGGCCCGCGTGGTTGTTTTAATAGCACCGCCTGCTTTCTGATCTACCGCAAATGCAAATCCCCCCTCTTTAACCTCCAGTAGTCCTCCCTTTTCGATAGCGACAAAGACg‑g                                                                                                                                         >  2:7043788/1‑140 (MQ=255)

  cGAGACGGTTTGTTCCGAATACCTCCATGGCCCGCGTGGTTGTTTTAATAGCACCGCCTGCTTTCTGATCTACCGCAAATGCAAATCCCCCCTCTTTAACCTCCAGTAGTCCTCCCTTTTCGATAGCGACAAAGACG‑G‑cc                                                                                                                                      >  2:3799928/1‑140 (MQ=255)

          tttGTTCCGAATACCTCCATGGCCCGCGTGGTTGTTTTAATAGCACCGCCTGCTTTCTGATCTACCGCAAATGCAATTCCCCCCTCTTTAACCTCCAGTAGTCCTCCCTTTTCGATAGCGACAAAGACG‑G‑cccc‑cccatc                                                                                                                             >  1:3121964/1‑136 (MQ=255)

          tttGTTCCGAATACCTCCATGGCCCGCGTGGTTGTTTTAATAGCACCGCCTGCTTTCTGATCTACCGCAAATGCAAATCCCCCCTCTTTAACCTCCAGTAGTCCTCCCTTTTCGATAGCGACAAAGACG‑G‑cccc‑cccatc                                                                                                                             >  2:1273953/1‑136 (MQ=255)

               tCCGAATACCTGCATGGCCCGCGTGGTGGTTTTAATAGCACCGCCTGCTTTCTGATCTACCGCAAATGCACATCCCCCCTCGTTATCCTCCAGTAGTCCTCCCTTTTCGATAGCGACAAAGACG‑GCCCCC‑CCATCtattt                                                                                                                         >  2:4501361/1‑140 (MQ=255)

                 cGAATACCTCCATGGCCCGCGTGGTTGTTTTAATAGCACCGCCTGCTTTCTGATCTACCGCAAATGCAAATCCCCCCTCTTTAACCTCCAGTAGTCCTCCCTTTTCGATAGCGACAAAGACG‑GCCCCC‑CCATCTTTTTCa                                                                                                                       <  1:8782132/140‑1 (MQ=255)

                 cGAATACCTCCATGGCCCGCGTGGTTGTTTTAATAGCACCGCCTGCTTTCTGATCTACCGCAAATGCAAATCCCCCCTCTTTAACCTCCAGTAGTCCTCCCTTTTCGATAGCGACAAAGACG‑GCCCCC‑CCATCTTTTTCa                                                                                                                       <  1:4696475/140‑1 (MQ=255)

                  gAATACCTCCATGGCCCGCGTGGTTGTTTTAATAGCACCGCCTGCTTTCTGATCTACCGCAAATGCAAATCCCCCCTCTTTAACCTCCAGTAGTCCTCCCTTTTCGATAGCNACAAAGACG‑GCCCCC‑CCATCTTTTTCaa                                                                                                                      >  1:6253186/1‑140 (MQ=255)

                  gAATACCTCCATGGCCCGCGTGGTTGTTTTAATAGCACCGCCTGCTTTCTGATCTACCGCAAATGCAAATCCCCCCTCTTTAACCTCCAGTAGTCCTCCCTTTTCGATAGCGACAAAGACG‑GCCCCC‑CCATCTTTTTCaa                                                                                                                      <  1:5248600/140‑1 (MQ=255)

                   aaTACCTCCATGGCCCGCGTGGTTGTTTTAATAGCACCGCCTGCTTTCTGATCTACCGCAAATGCAAATCCCCCCTCTTTAACCTCCAGTAGTCCTCCCTTTTCGATAGCGACAAAGACG‑GCCCCC‑CCATCTTTTTCAAc                                                                                                                     >  1:7073565/1‑140 (MQ=255)

                   aaTACCTCCATGGCCCGCGTGGTTGTTTTAATAGCACCGCCTGCTTTCTGATCTACCGCAAATGCAAATCCCCCCTCTTTAACCTCCAGTAGTCCTCCCTTTTCGATAGCGACAAAGACG‑GCCCCC‑CCATCTTTTTCAAc                                                                                                                     <  1:1602584/140‑1 (MQ=255)

                        cTCCATGGCCCGCGTGGTTGTTTTAATAGCACCGCCTGCTTTCTGATCTACCGCAAATGCAAATCCCCCCTCTTTAACCTCCAGTAGTCCTCCCTTTTCGATAGCGACAAAGACG‑GCCCCC‑CCATCTTTTTCAACGATTa                                                                                                                >  2:3523071/1‑140 (MQ=255)

                        cTCCATGGCCCGCGTGGTTGTTTTAATAGCACCGCCTGCTTTCTGATCTACCGCAAATGCAAATCCCCCCTCTTTAACCTCCAGTAGTCCTCCCTTTTCGATAGCGACAAAGACG‑GCCCCC‑CCATCTTTTTCAACGATTa                                                                                                                >  2:2806806/1‑140 (MQ=255)

                             tGGCCCGCGTGGTTGTTTTAATAGCACCGCCTGCTTTCTGATCTACCGCAAATGCAACTCCCCCCTCTTTAACCTCCAGTAGTCCTCCCTTTTCGATAGCGACAAAGACG‑GCCCCC‑CCATCTTTTTCAACGATTAAgcgc                                                                                                           >  1:8170759/1‑140 (MQ=255)

                             tGGCCCGCGTGGTTGTTTTAATAGCACCGCCTGCTTTCTGATCTACCGCAAATGCAAATCCCCCCTCTTTAACCTCCAGTAGTCCTCCCTTTTCGATCGCGACAAAGACG‑GCCCCC‑CCATCTTATTCAACGATTAAgcgc                                                                                                           >  2:8386695/1‑140 (MQ=255)

                              ggCCCGCGTGGTTGTTTTAATAGCACCGCCTGCTTTCTGATCTACCGCAAATGCAAATCCCCCCTCTTTAACCTCCAGTAGTCCTCCCTTTTCGATAGCGACAAAGACG‑GCCCCC‑CCATCTTTTTCAACGATTAAGcgcc                                                                                                          <  1:1360367/140‑1 (MQ=255)

                              ggCCCGCGTGGTTGTTTTAATAGCACCGCCTGCTTTCTGATCTACCGCAAATGCAAATCCCCCCTCTTTAACCTCCAGTAGTCCTCCCTTTTCGATAGCGACAAAGACG‑GCCCCC‑CCATCTTTTTCAACGATTAAGcgcc                                                                                                          <  1:393404/140‑1 (MQ=255)

                              ggCCCGCGTGGTTGTTTTAATAGCACCGCCTGCTTTCTGATCTACCGCAAATGCAAATCCCCCCTCTTTAACCTCCAGTAGTCCTCCCTTTTCGATAGCGACAAAGACG‑GCCCCC‑CCATCTTTTTCAACGATTAAGcgcc                                                                                                          <  1:6370859/140‑1 (MQ=255)

                              ggCCCGCGTGGTTGTTTTAATAGCACCGCCTGCTTTCTGATCTACCGCAAATGCAAATCCCCCCTCTTTAACCTCCAGTAGTCCTCCCTTTTCGATAGCGACAAAGACG‑GCCCCC‑CCATCTTTTTCAACGATTAAGcgcc                                                                                                          <  1:5708047/140‑1 (MQ=255)

                               gCCCGCGTGGTTGTTTTAATAGCACCGCCTGCTTTCTGATCTACCGCAAATGCAAATCCCCCCTCTTTAACCTCCAGTAGTCCTCCCTTTTCGATAGCGACAAAGACG‑GCCCCC‑CCATCTTTTTCAACGATTAAGcgccc                                                                                                         <  2:4017957/140‑1 (MQ=255)

                                cccGCGTGGTTGTTTTAATAGCACCGCCTGCTTTCTGATCTACCGCAAATGCAAATCCCCCCTCTTTAACCTCCAGTAGTCCTCCCTTTTCGATAGCGACAAAGACG‑GCCCCC‑CCATCTTTTTCAACGATTAAGcgcccg                                                                                                        >  1:7533354/1‑140 (MQ=255)

                                  cgcgTGGTTGTTTTAATAGCACCGCCTGCTTTCTGATCTACCGCAAATGCAAATCCCCCCTCTTTAACCTCCAGTAGTCCTCCCTTTTCGATAGCGACAAAGACG‑GCCCCC‑CCATCTTTTTCAACGATTAAGcgcccgcc                                                                                                      >  1:5020459/1‑140 (MQ=255)

                                    cgTGGTTGTTTTAATAGCACCGCCTGCTTTCTGATCTACCGCAAATGCAAATCCCCCCTCTTTAACCTCCAGTAGTCCTCCCTTTTCGATAGCGACAAAGACG‑GCCCCC‑CCATCTTTTTCAACGATTAAGCGCCCGCCtt                                                                                                    >  2:367532/1‑140 (MQ=255)

                                    cgTGGTTGTTTTAATAGCACCGCCTGCTTTCTGATCTACCGCAAATGCAAATCCCCCCTCTTTAACCTCCAGTAGTCCTCCCTTTTCGATAGCGACAAAGACG‑GCCCCC‑CCATCTTTTTCAACGATTAAGCGCCCGCCtt                                                                                                    >  1:5047921/1‑140 (MQ=255)

                                    cgTGGTTGATTTAATAGCACCGCCTGCTTTCTGATATACAGAAAATGCAAATCACCCCTCTGTAACATACAGTAGTCCTCACTTTTCGATAGCGACAAAGACG‑GCCCCC‑CCATCTTTTTCAACTATTAAGAGACCGACtt                                                                                                    >  2:599344/1‑140 (MQ=255)

                                     gTGGTTGTTTTAATAGCACCGCCTGCTTTCTGATCTACCGCAAATGCAAATCCCCCCTCTTTAACCTCCAGTAGTCCTCCCTTTTCGATAGCGACAAAGACG‑GCCCCC‑CCATCTTTTTCAACGATTAAGCGCCCGCCttt                                                                                                   >  1:3371929/1‑140 (MQ=255)

                                      tcgttgctTTACTAGCACCGCCCGCCTCCAGATCTCCCGCCAATGCAAATCCCCCCTCTTCACCCACCAGGAGTCCCCCCTTGTCGAGCGCGACAAAGACG‑GCCCCC‑CCATCTATATCAACGATGAAGCGCCCGCCTTTa                                                                                                  <  2:5878520/138‑1 (MQ=255)

                                          tgttTTAATAGCACCGCCTGCTTTCTGATCTACCGCAAATGCAAATCCCCCCTCTTTAACCTCCAGTAGTCCTCCCTTTTCGATAGCGACAAAGACG‑GCCCCC‑CCATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCg                                                                                              <  1:2560837/140‑1 (MQ=255)

                                          tgatttAATAGCACCGCCTGCTTTCTGATCTACCGCAAATGCAAATCCCCCCTCTTTAACCTCCAGTAGTCCTCCCTTTTCGATAGCGACAAAGACG‑GCCCCC‑CCATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCg                                                                                              <  2:3340471/137‑1 (MQ=255)

                                            ttttAATAGCACCGCCTGCTTTCTGATCTACCGCAAATGCAAATCCCCCCTCTTTAACCTCCAGTAGTCCTCCCTTTTCGATAGCGACAAAGACG‑GCCCCC‑CCATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTg                                                                                            <  2:1976301/140‑1 (MQ=255)

                                                     cACCGCCTGCTTTCTGATCTACCGCAAATGCAAATCCCCCCTCTTTAACCTCCAGTAGTCCTCCCTTTTCGATAGCGACAAAGACG‑GCCCCC‑CCATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTc                                                                                   >  1:2899612/1‑140 (MQ=255)

                                                       ccGCCTGCTTTCTGATCTACCGCAAATGCAAATCCCCCCTCTTTAACCTCCAGTAGTCCTCCCTTTTCGATAGCGACAAAGACG‑GCCCCC‑CCATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGc                                                                                 >  1:341952/1‑140 (MQ=255)

                                                        cGCCTGCTTTCTGATCTACCGCAAATGCAAATCCCCACTCTTTAACCTCCAGTAGTCCTCCCTTTTCGATAGCGACAAAGACG‑GCCCCC‑CCATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCt                                                                                <  2:411931/140‑1 (MQ=255)

                                                         gCCTGCTTTCTTATCTACCGCAAATGCAAATCCCCCCTCTTTAACCTCCAGTAGGCCTCCCTTTTCGAGAGCGACAAAGACG‑GCCCCC‑CCATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCTg                                                                               <  2:3169320/140‑1 (MQ=255)

                                                         gCCTGCTTTCTGATCTACCGCAAATGCAAATCCCCCCTCTTTAACCTCCAGTAGTCCTCCCTTTTCGATAGCGACAAAGACG‑GCCCCC‑CCATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCTg                                                                               <  2:4790960/140‑1 (MQ=255)

                                                         gCCTGCTTTCTGATCTACCGCAAATGCAAATCCCCCCTCTTTAACCTCCAGTAGTCCTCCCTTTTCGATAGCGACAAAGACG‑GCCCCC‑CCATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCTg                                                                               <  2:2105093/140‑1 (MQ=255)

                                                         gCCTGCTTTCTGATCTACCGCAAATGCAAATCCCCCCTCTTTAACCTCCAGTAGTCCTCCCTTTTCGATAGCGACAAAGACG‑GCCCCC‑CCATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCTg                                                                               <  1:8347964/140‑1 (MQ=255)

                                                         gCCTGCTTACTGATCTACCGCACATGCAAATCCCCCCTCTTTCCCCTCCAGTAGTCCTCCCTTTTCGATAGCGACAAAGACG‑GCCCCC‑CCATCTTTTTCAACGATTAAGCGCCCGCCTATAATCGTGGTATCATGCGCTg                                                                               <  2:6888235/140‑1 (MQ=255)

                                                          ccTGCTTTCTGATCTACCGCAACTGCAAATCCCCCCTCTTTAACCTCCAGTAGTCCTCCCTTTTCGATAGCGACAAAGACG‑GCCCCC‑CCATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCTgc                                                                              >  2:2644414/1‑140 (MQ=255)

                                                          ccTGCTTTCTGATCTACCGCAAATGCAAATCCCCCCTCTTTAACCTCCAGTAGTCCTCCCTTTTCGATAGCGACAAAGACG‑GCCCCC‑CCATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCTgc                                                                              >  1:4002076/1‑140 (MQ=255)

                                                             gCTTTCTGATCTACCGCAAATGCAAATCCCCCCTCTTTAACCTCCAGTAGTCCTCCCTTTTCGATAGCGACAAAGACG‑GCCCCC‑CCATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGGATCATTCGCTGCGcc                                                                           >  1:9331111/1‑140 (MQ=255)

                                                              cTTTCTGATCTACCGCAAATGCAAATCCCCCCTCTTTAACCTCCAGTAGTCCTCCCTTTTCGATAGCGACAAAGACG‑GCCCCC‑CCATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCTGCGCCa                                                                          <  1:3794129/140‑1 (MQ=255)

                                                              cTTTCTGATCTACCGCAAATGCAAATCCCCCCTCTTTAACCTCCAGTAGTCCTCCCTTTTCGATAGCGACAAAGACG‑GCCCCC‑CCATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCTGCGCCa                                                                          >  1:764437/1‑140 (MQ=255)

                                                               tttCTGATCTACCGCAAATGCAAATCCCCCCTCTTTAACCTACAGTAGTCCTCCCTTTTCGATAGCGACAAAGACG‑GCCCCC‑CCATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCTGCGCCAt                                                                         <  2:4364501/140‑1 (MQ=255)

                                                                 tCTGATCTACCGCAAATGCAAATCCCCCCTCTTTAACCTCCAGTAGTCCTCCCTTTTCGATAGCGACAAAGACG‑GCCCCC‑CCATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCTGCGCCATaa                                                                       <  1:3493604/140‑1 (MQ=255)

                                                                 tCTGATCTACCGCAAATGCAAATCCCCCCTCTTTAACCTCCAGTAGTCCTCCCTTTTCGATAGCGACAAAGACG‑GCCCCC‑CCATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCTGCGCCATaa                                                                       <  1:6518913/140‑1 (MQ=255)

                                                                   tGATCTACCGCAAATGCAAATCCCCCCTCTTTAACCTCCAGTAGTCCTCCCTTTACGATAGCGACAAAGACG‑GCCCCC‑CCATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCTGCGCCATAAAc                                                                     <  1:1009217/140‑1 (MQ=255)

                                                                    gATCTACCGCAAATGCAAATCCCCCCTCTTTAACCTCCAGTAGTCCTCCCTTTTCGATAGCGACAAAGACG‑GCCCCC‑CCATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCTGCGCCATAAAcc                                                                    <  1:99720/140‑1 (MQ=255)

                                                                     aTCTACCGCAAATGCAAATCCCCCCTCTTTAACCTCCAGTAGTCCTCCCTTTTCGATAGCGACAAAGACG‑GCCCCC‑CCATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCTGCGCCATAAACCt                                                                   >  2:2499401/1‑140 (MQ=255)

                                                                         aCCGCAAATGCAAATCCCCCCTCTTTAACCTCCAGTAGTCCTCCCTTTTCGATAGCGACAAAGACGAC‑CCCC‑CCATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCTGCGCCATAAACCTGTAg                                                               <  1:4477197/140‑1 (MQ=255)

                                                                         aCCGCAAATGCAAATCCCCCCTCTTTAACCTCCAGTAGTCCTCCCTTTTCGATAGCGACAAAGACG‑GCCCCC‑CCATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCTGCGCCATAAACCTGTAg                                                               <  2:6162993/140‑1 (MQ=255)

                                                                         aCCGCAAATGCAAATCCCCCCTCTTTAACCTCCAGTAGTCCTCCCTTTTCGATAGCGACAAAGACG‑GCCCCC‑CCATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCTGCGCCATAAACCTGTAg                                                               <  2:3604160/140‑1 (MQ=255)

                                                                           cGCAAATGCAACTCCCCCCTCTTTAACCTCCAGTAGTCCTCCCATGTCGATAGCGACAAAGACG‑GCCCCC‑CCATCATTTTCAACGATTAAGCGCCCGCCATTAATCGTGGTATCATTCGCTGCGCCATAAACCTGTAGca                                                             <  2:790141/140‑1 (MQ=255)

                                                                           cGCAAATGCAAATCCCCCCTCTTTAACCTCCAGTAGTCCTCCCTTTTCGATAGCGACAAAGACG‑GCCCCC‑CCATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCTGCGCCATAAACCTGTAGca                                                             >  2:1839323/1‑140 (MQ=255)

                                                                           cGCAAATGCAAATCCCCCCTCTTTAACCTCCAGTAGTCCTCCCTTTTCGATAGCGACAAAGACG‑GCCCCC‑CCATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCTGCGCCATAAACCTGTAGca                                                             <  2:6356328/140‑1 (MQ=255)

                                                                           cGCAAATGCAAATCCCCCCTCTTTAACCTCCAGTAGTCCTCCCTTTTCGATAGCGACAAAGACG‑GCCCCC‑CCATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCTGCGCCATAAACCTGTAGca                                                             <  2:4577884/140‑1 (MQ=255)

                                                                           cGCAAATGCAAATCCCCCCTCTTTAACCTCCAGTAGTCCTCCCTTTTCGATAGCGACAAAGACG‑GCCCCC‑CCATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCTGCGCCATAAACCTGTAGca                                                             <  1:7913688/140‑1 (MQ=255)

                                                                           cGCAAATGCAAATCCCCCCGCTTTAACCTCCAGTAGTCCTCCCTTTTCGATAGCGACAAAGACG‑GCCCCC‑CCATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCTGCGCCATAAACCTGTAGca                                                             <  2:7190126/140‑1 (MQ=255)

                                                                             caaatgcaaatCCCCCCTCTTTAACCTCCAGTAGTCCTCCCTTTTCGATAGCGACAAAGACG‑G‑CCCCACCATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCTGCGCCATAAACCTGTAGcaca                                                           >  1:6619424/1‑140 (MQ=255)

                                                                              aaatgcaaatCCCCCCTCTTTAACCTCCAGTAGTCCTCCCTTTTCGATAGCGACAAAGACG‑GCCCCC‑CCATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCTGCGCCATAAACCTGTAGCacac                                                          <  1:4275688/140‑1 (MQ=255)

                                                                                atgcaaatCCCCCCTCTTAAACCTCCAGTAGTCCTCCATTTTCGATAGCGACAAAGACG‑GCCCCC‑CCATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCTGCTCCATAAACCTGTAGCACACCa                                                        <  2:2197585/140‑1 (MQ=255)

                                                                                  gcaaatCCCCCCTCTTTAACATCCAGTAGTCCTCCCTTTTCGATAGCGACAAAGACG‑GCCCCC‑CCATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCTGCGCCATAAACCTGTAGCACACCatt                                                      >  2:8712891/1‑140 (MQ=255)

                                                                                    aaatCCCCCCTCTTTAACCTCCAGTAGTCCTCCCTTTTCGATAGCGACAAAGACG‑GCCCCC‑CCATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCTGCGCCATAAACCTGTAGCACACCattat                                                    <  2:341952/140‑1 (MQ=255)

                                                                                       tCCCCCCTCTTTAACCTCCAGTAGTCCTCCCTTTTCGATAGCGACAAAGACG‑GCCCCC‑CCATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCTGCGCCATAAACCTGTAGCACACCATTATTGa                                                 <  2:8246922/140‑1 (MQ=255)

                                                                                         cccccTCTTTAACCTCCAGTAGTCCTCCCTTTTCGATAGCGACAAAGACG‑GCCCCC‑CCATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCTGCGCCATAAACCTGTAGCACACCATTATTGAGc                                               <  1:2394003/140‑1 (MQ=255)

                                                                                          ccccTCTTTAACCTCCAGTAGTCCTCCCTTTTCGATAGCGACAAAGACG‑GCCCCC‑CCATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCTGCGCCATAAACCTGTAGCACACCATTATTGAGCg                                              <  1:1805347/140‑1 (MQ=255)

                                                                                          ccccTCTTTAACCTCCAGTAGTCCTCCCTTTTCGATAGCGACAAAGACG‑GCCCCC‑CCATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCTGCGCCATAAACCTGTAGCACACCATTATTGAGCg                                              <  1:4848893/140‑1 (MQ=255)

                                                                                            ccTCTTTAACCTCCAGTAGTCCTCCCTTTTCGATAGCGACAAAGACG‑GCCCCC‑CCATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCTGCGCCATAAACCTGTAGCACACCATTATTGAGCGTa                                            <  2:2991179/140‑1 (MQ=255)

                                                                                              tctTTAACCTCCAGTAGTCCTCCCTTTTCGATAGCGACAAAGACG‑GCCCCC‑CCATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCTGCGCCATAAACCTGTAGCACACCATTATTGAGCGTAGt                                          <  2:1648453/140‑1 (MQ=255)

                                                                                                  tAACCTCCAGTAGTCCTCCCTTTTCGATAGCGACAAAGACG‑GCCCCC‑CCATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCTGCGCCATAAACCTGTAGCACACCATTATTGAGCGTAGTCTgg                                      >  1:4160866/1‑140 (MQ=255)

                                                                                                    aCCTCCAGTAGTCCTCCCTTTTCGATAGCGACAAAGACG‑GCCCCC‑CCATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCTGCGCCATAAACCTGTAGCACACCATTATTGAGCGTAGTCTGGtt                                    >  2:8031475/1‑140 (MQ=255)

                                                                                                         cAGTAGTCCTCCCTTTTCGATAGCGACAAAGACG‑GCCCCC‑CCATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCTGCGCCATAAACCTGTAGCACACCATTATTGAGCGTAGTCTGGTTTGCcg                               >  1:9039282/1‑140 (MQ=255)

                                                                                                          agtagtCCTCCCTTTTCGATAGCGACAAAGACG‑GCCCCC‑CCATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCTGCGCCATAAACCTGTAGCACACCATTATTGAGCGTAGTCTGGTTTGCcgc                              >  1:352771/1‑140 (MQ=255)

                                                                                                            tagtCCTCCCTTTTCGATAGCGACAAAGACG‑GCCCCC‑CCATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCTGCGCCATAAACCTGTAGCACACCATTATTGAGCGTAGTCTGGTTTGCCgcgc                            <  1:4603376/140‑1 (MQ=255)

                                                                                                              gtCCTCCCTTTTCGATAGCGACAAAGACG‑GCCCCC‑CCATCTTTTTCAACGATTAAGCTCCCGCCTTTAATCGTGGTATCATTCGCTGCGCCATAAACCTGTAGCACACCATTATTGAGCGTAGTCTGGTTTGCCGCGCCa                          <  2:8540462/140‑1 (MQ=255)

                                                                                                              gtCCTCCCTTTTCGATAGCGACAAAGACG‑GCCCCC‑CCATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCTGCGCCATAAACCTGTAGCACACCATTATTGAGCGTAGTCTGGTTTGCCGCGCCa                          <  1:681415/140‑1 (MQ=255)

                                                                                                              gtCCTCCCTTTTCGATAGCGACAAAGACG‑GCCCCC‑CCATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCTGCGCCATAAACCTGTAGCACACCATTATTGAGCGTAGTCTGGTTTGCCGCGCCa                          <  1:2253837/140‑1 (MQ=255)

                                                                                                                 ctccCTTTTCGATAGCGACAAAGACG‑GCCCCC‑CCATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCTGCGCCATAAACCTGTAGCACACCATTATTGAGCGTAGTCTGGTTTGCCGCGCCAccc                       >  2:9343823/1‑140 (MQ=255)

                                                                                                                 ctccCTTTTCGATAGCGACAAAGACG‑GCCCCC‑CCATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCTGCGCCATAAACCTGTAGCACACCATTATTGAGCGTAGTCTGGTTTGCCGCGCCAccc                       >  1:849258/1‑140 (MQ=255)

                                                                                                                 ctccCTTTTCGATAGCGACAAAGACG‑GCCCCC‑CCATCTTTTTCAACGATTAAGCGACCGCCTTTAATCGTGGTATCATTCGCTGCGCCATAAACCTGTAGCACACCATTATTGTGCGTAGTCTGGTTTGCCGCGCCAccc                       >  2:646335/1‑140 (MQ=255)

                                                                                                                   cccTTTTCGATAGCGACAAAGACG‑GCCCCC‑CCATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCTGCGCCATAAACCTGTAGCACACCATTATTGAGCGTAGTCTGGTTTGCCGCGCCAcccct                     >  1:6902833/1‑138 (MQ=255)

                                                                                                                   cccTTTTCGATAGCGACAAAGACG‑GCCCCC‑CCATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCTGCGCCATAAACCTGTAGCACACCATTATTGAGCGTAGTCTGGTTTGCCGCGCCACCCAt                     >  2:564893/1‑140 (MQ=255)

                                                                                                                   cccATTCCGATAGCGACAACGACG‑GCCCCC‑CCACCTTTTACAACGATTACGCGCCCGCCTTTAATCGTGGTATCAGGCGCTGCGCCATACCCCTGTAGCACACCATTCTTGAGCGTAGTCTGGTTTGCCGCGCCACCCag                     <  2:5041729/140‑2 (MQ=255)

                                                                                                                      ttttCGATAGCGACAAAGACG‑GCCCCC‑CCATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCTGCGCCATAAACCTGTAGCACACCATTATTGAGCGTAGTCTGGTTTGCCGCGCCACCCATTTg                  <  1:5547690/140‑1 (MQ=255)

                                                                                                                        ttCGATAGCGACAAAGACG‑GCCCCC‑CCATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCTGCGCCATAAACCTGTAGCACACCATTATTGAGCGTAGTCTGGTTTGCCGCGCCACCCATTTGaa                <  1:6063788/140‑1 (MQ=255)

                                                                                                                           gATAGCGACAAAGACG‑GCCCCC‑CCATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCTGCGCCATAAACCTGTAGCACACCATTATTGAGCGTAGTCTGGTTTGCCGCGCCACCCATTTGAACaa             <  1:7771428/140‑1 (MQ=255)

                                                                                                                              aGCGACAAAGACG‑GCCCCC‑CCATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCTGCGCCATAAACCTGTAGCACACCATTATTGAGCGTAGTCTGGTTTGCCGCGCCACCCATTTGAACAAttt          >  1:112303/1‑140 (MQ=255)

                                                                                                                                 gACAAAGACG‑GCCCCC‑CCATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCTGCGCCATAAACCTGTAGCACACCATTATTGAGCGTAGTCTGGTTTGCCGCGCCACCCATTTGAACAATTTGAt       <  2:4999881/140‑1 (MQ=255)

                                                                                                                                    aaaGACG‑GCCCCC‑CCATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCTGCGCCATACACCTGTAGCACACCAGTATTGAGCGTAGTCTGGTTTGCCGCGCCACCCATTTGAACAATTTGATAAc    <  2:896201/140‑1 (MQ=255)

                                                                                                                                      aGACG‑GCCCCC‑CCATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCTGCGCCATAAACCTGTAGCACACCATTATTGAGCGTAGTCTGGTTTGCCGCGCCACCCATTTGAACAATTTGATAACCg  <  1:7339454/140‑1 (MQ=255)

                                                                                                                                      aGACG‑GCCCCC‑CCATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCTGCGCCATAAACCTGTAGCACACCATTATTGAGCGTAGTCTGGTTTGCCGCGCCACCCATTTGAACAATTTGATAACCg  <  2:7446712/140‑1 (MQ=255)

                                                                                                                                      aGACG‑GCCCCC‑CCATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCTGCGCCATAAACCTGTAGCACACCATTATTGAGCGTAGTCTGGTTTGCCGCGCCACCCATTTGAACAATTTGATAACCg  <  2:7797950/140‑1 (MQ=255)

                                                                                                                                      aGACG‑GCCCCC‑CCATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCTGCGCCATAAACCTGTAGCACACCATTATTGAGCGTAGTCTGGTTTGCCGCGCCACCCATTTGAACAATTTGATAACCg  <  2:1562403/140‑1 (MQ=255)

                                                                                                                                      aGACG‑GCCCCC‑CCATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCTGCGCCATAAACCTGTAGCACACCATTATTGAGCGTAGTCTGGTTTGCCGCGCCACCCATTTGAACAATTTGATAACCg  <  1:5905399/140‑1 (MQ=255)

                                                                                                                                      aGACG‑GCCCCC‑CCATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCTGCGCCATAAACCTGTAGCACACCATTATTGAGCGTAGTCTGGTTTGCCGCGCCACCCATTTGAACAATTTGATAACCg  <  1:5412960/140‑1 (MQ=255)

                                                                                                                                      aGACG‑GCCCCC‑CCATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCTGCGCCATAAACCTGTAGCACACCATTATTGAGCGTAGTCTGGTTTGCCGCGCCACCCATTTGAACAATTTGATAACCg  <  1:2806806/140‑1 (MQ=255)

                                                                                                                                      aGACG‑GCCCCC‑CCATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCTGCGCCATAAACCTGTAGCACACCATTATTGAGCGTAGTCTGGTTTGCCGCGCCACCCATTTGAACAATTTGATAACCg  <  1:1647141/140‑1 (MQ=255)

                                                                                                                                      aGACG‑GCCCCC‑CCATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCTGCGCCATAAACCTGTAGCACACCATTATTGAGCGTAGTCTGGTTTGCCGCGCCACCCATTTGAACAATTTGATAACCg  <  2:5778247/140‑1 (MQ=255)

                                                                                                                                      aGACG‑GCCCCC‑CCATCTATTTCAACGATTAAGCGCCCGCCTTTAATCGGGGTATCATACGCTGCGCCATACACCAGTAGCACACCAATATTGAGCGTAGTCTGGTTTGCCGCGCCACCCATTTGAACAATGTGATACCCg  <  2:6630600/140‑1 (MQ=255)

ACCGAGACGGTTTGTTCCGAATACCTCCATGGCCCGCGTGGTTGTTTTAATAGCACCGCCTGCTTTCTGATCTACCGCAAATGCAAATCCCCCCTCTTTAACCTCCAGTAGTCCTCCCTTTTCGATAGCGACAAAGACG‑G‑CCCC‑CCATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCTGCGCCATAAACCTGTAGCACACCATTATTGAGCGTAGTCTGGTTTGCCGCGCCACCCATTTGAACAATTTGATAACCG  >  NZ_CP025268/2292451‑2292723

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`