BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP025268	2,769,852	+C	intergenic (‑52/‑89)	yfjM ← / → rnlA	DUF1524 domain‑containing protein/type II toxin‑antitoxin system toxin mRNA endoribonuclease RnlA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP025268	2,769,848	1	.	C	95.3%	1548.7 / 62.3	472	intergenic (‑48/‑93)	yfjM/rnlA	DUF1524 domain‑containing protein/type II toxin‑antitoxin system toxin mRNA endoribonuclease RnlA
Reads supporting (aligned to +/- strand): ref base . (4/18); new base C (192/255); total (197/275)
Fisher's exact test for biased strand distribution p-value = 2.56e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 490 total aligned reads displayed.

CTTGTCTGCTGGCGAATAATTACTAAGTATCGAGTTGCTGCTTGGTGAGATCAGGAAGAGGTTACCGAAACGATGGACATCATCCCCCATTTTGTGGACTGCCCCCCCGGTAGACGATCCTGCCCTATAGTTGGAGTG‑CCC‑C‑TATTCAGGTGGCTAAATTTAGCAAATCAATACACTTCAGGGGGGTATTATTGTAGAGTTTCCCCATATGTTTCTATGGGATCCAGGAAATGACAATCAGGAGTTACAAAAACTTAAATCTGGTCAGGGCAA  >  NZ_CP025268/2769711‑2769983

cTTGTCTGCTGGCGAATAATTACTAAGTATCGAGTTGCTGCTTGGTGAGATCTGGAAGAGGTTACCGAAACGATGGACATCATCCCCCATTTTGTGGACTGCCCCCCCGGTAGACGATCCTGCCCTATAGTTGGTGTG‑cc                                                                                                                                         <  1:9231707/140‑1 (MQ=255)

cTTGTCTGCTGGCGAATAATTACTAAGTATCGAGTTGCTGCTTGGTGAGATCAGGAAGAGGTTACCGAAACGATGGACATCATCCCCCATTTTGTGGACTGCCCCCCCGGTAGACGATCCTGCCCTATAGTTGGAGTG‑cc                                                                                                                                         <  1:3822073/140‑1 (MQ=255)

cTTGTCTGCTGGCGAATAATTACTAAGTATCGAGTTGCTGCTTGGTGAGATCAGGAAGAGGTTACCGAAACGATGGACATCATCCCCCATTTTGTGGACTGCCCCCCCGGTAGACGATCCTGCCCTATAGTTGGAGTG‑cc                                                                                                                                         <  1:3533315/140‑1 (MQ=255)

        ctgGCGAATAATTACTAAGTATCGAGTTGCTGCTTGGTGAGATCAGGAAGAGGTTACCGAAACGATGGACATCATCCCCCATTTTGTGGACTGCCCCCCCGGTAGACGATCCTGCCCTATAGTTGGAGTGCCCC‑C‑TATTc                                                                                                                                <  1:8238978/140‑1 (MQ=255)

          ggCGAATAATTACTAAGTATCGAGTTGCTGCTTGGTGAGATCAGGAAGAGGTTACCGAAACGATGGACATCATCCCCCATTTTGTGGACTGCCCCCCCGGTAGACGATCCTGCCCTATAGTTGGAGTGCCCC‑C‑TATTCAg                                                                                                                              <  1:7662185/140‑1 (MQ=255)

              tataatTACTAAGTATCTAGTTGCTGCTTGGTGAGTTCAGTAAGAGTTTACCGAAACGATGGAAATCATCCCCCATTTTTTTGACTGCCCCCCCGGTAGACGATCCTGCCCTATAGTTGGAGTGCCCC‑C‑TATTCAGGTgg                                                                                                                          <  2:6558090/139‑1 (MQ=255)

                  atTACTAAGTATCGAGTTGCTGCTTGGTGAGATCAGGAAGAGGTTACCGAAACGATGGACATCATCCCCCATTTTGTGGACTGCCCCCCCGGTAGACGATCCTGCCCTATCGTTGGAGTGCCCC‑C‑GATTCAGGTGGCtca                                                                                                                      >  2:3326313/1‑138 (MQ=255)

                     aCTAAGTATCGAGTTGCTGCTTGGTGAGATCAGGCAGAGGTTACCGAAACGATGGACATCATCCCCCATTGTGTGGACTGCCCCCCCGGTCGACGATCCTGCCCTCTAGTTGGAGTGCCCC‑C‑TATTCAGGTGGCTAAAtt                                                                                                                   >  2:8604573/1‑140 (MQ=255)

                     aCTAAGTATCGAGTTGCTGCTTGGTGAGATCAGGAAGAGGTTACCGAAACGATGGACATCATCCCCCATTTTGTGGACTGCCCCCCCGGTCGTCGATCCTGCCCGATAGTTGGAGTGCCCC‑C‑TATTCAGGTGGCTAAAtt                                                                                                                   >  2:4993173/1‑140 (MQ=255)

                        aaGTATCGAGTTGCTGCTTGGTGAGATCAGGAAGAGGTTACCGAAACGATGGACATCATCCCCCATTTTGTGGACTGCCCCCCCGGTAGACGATCCTGCCCTATAGTTGGAGTGCCCC‑C‑TATTCAGGTGGCTAAATTTAg                                                                                                                <  1:5063266/140‑1 (MQ=255)

                        aaGTATCGAGTTGCTGCTTGGTGAGATCAGGAAGAGGTTACCGAAACGATGGACATCATCCCCCATTTTGTGGACTGCCCCCCCGGTAGACGATCCTGCCCTATAGTTGGAGTGCCCC‑C‑TATTCAGGTGGCTAAATTTAg                                                                                                                <  1:3375752/140‑1 (MQ=255)

                        aaGTATCGAGTTGCTGCTTGGTGAGATCAGGAAGAGGTTACCGAAACGATGGACATCATCCCCCATTTTGTGGACTGCCCCCCCGGTAGACGATCCTGCCCTATAGTTGGAGTGCCCC‑C‑TATTCAGGTGGCTAAATTTAg                                                                                                                <  1:5813643/140‑1 (MQ=255)

                        aaGTATCGAGTTGCTGCTTGGTGAGATCAGGAAGAGGTTACCGAAACGATGGACATCATCCCCCATTTTGTGGACTGCCCCCCCGGTAGACGATCCTGCCCTATAGTTGGAGTGCCCC‑C‑TATTCAGGTGGCTAAATTTAg                                                                                                                <  1:7198718/140‑1 (MQ=255)

                        aaGTATCGAGTTGCTGCTTGGTGAGATCAGGAAGAGGTTACCGAAACGATGGACATCATCCCCCATATTGTGGACTGCCCCCCCGGTAGACGATCCGGCCCTATAGTTGGAGTGCCCC‑C‑TATTCAGGTGGCTAAATTGAg                                                                                                                <  2:3069352/140‑1 (MQ=255)

                          gTATCGAGTTGCTGCTTGGTGAGATCAGGAAGAGGTTACCGAAACGATGGACATCATCCCCCATTTTGTGGACTGCCCCCCCGGTAGTCGATCCTGCCCTATAGTTGGAGTGCCCC‑C‑TATTCAGGTGGCTAAATTTAGCa                                                                                                              <  2:3401414/140‑1 (MQ=255)

                          gTATCGAGTTGCTGCTTGGTGAGATCAGGAAGAGGTTACCGAAACGATGGACATCATCCCCCATTTTGTGGACTGCCCCCCCGGTAGACGATCCTGCCCTATAGTTGGAGTGCCCC‑C‑TATTCAGGTGGCTAAATTTAGCa                                                                                                              <  1:6898626/140‑1 (MQ=255)

                             tCGAGTTGCTGCTTGGTGAGATCAGGAAGAGGTTACCGAAACGATGGACATCATCCCCCATTTTGTGGACTGCCCCCCCGGTAGACGATCCTGCCCTATAGTTGGAGTGCCCC‑C‑TATTCAGGTGGCTAAATTTAGCAAAt                                                                                                           <  1:3974079/140‑1 (MQ=255)

                                 gTTGCTGCTTGGTGAGATCAGGAAGAGGTTACCGAAACGATGGACATCATCCCCCATTTTGTGGACTGCCCCCCCGGTAGACGATCCTGCCCTATAGTTGGAGTGCCCC‑C‑TATTCAGGTGGCTAAATTTAGCAAATCAAt                                                                                                       <  1:5696818/140‑1 (MQ=255)

                                  ttGCTGCTTGGTGAGATCAGGAAGAGGTTACCGAATCGATGGACATCATCCCCCATTTTGTGGACTGCCCCCCCGGTAGACGATCCTGCCCTATAGTTGGAGTGCCCC‑C‑TATTCAGGTGGCTAAATTTAGCAAATCaaga                                                                                                      <  2:5394844/140‑3 (MQ=255)

                                  ttGCTGCTTGGTGAGATCAGGAAGAGGTTACCGAAACGATGGACATCATCCCCCATTTTGTGGACTGCCCCCCCGGTAGACGATCCTGCCCTATAGTTGGAGTGCCCC‑C‑TATTCAGGTGGCTAAATTTAGCAAATCAATa                                                                                                      <  2:6895451/140‑1 (MQ=255)

                                  ttGCTGCTTGGTGAGATCAGGAAGAGGTTACCGAAACGATGGACATCATCCCCCATTTTGTGGACTGCCCCCCCGGTAGACGATCCTGCCCTATAGTTGGAGTGCCCC‑C‑TATTCAGGTGGCTAAATTTAGCAAATCAATa                                                                                                      <  1:8567524/140‑1 (MQ=255)

                                  ttGCTGCTTGGTGAGATCAGGAAGAGGTTACCGAAACGATGGACATCATCCCCCATTTTGTGGACTGCCCCCCCGGTAGACGATCCTGCCCTATAGTTGGAGTGCCCC‑C‑TATTCAGGGGGCTAAATTTAGCAAATCAATa                                                                                                      <  2:2827481/140‑1 (MQ=255)

                                  ttGCTGCTTGGTGAGATCAGGAAGAGGTTACCGAAACGATGGACATCATCCCCCATTTTGTGGACTGCCCCCCCGGTAGACGATACTGCCCTATAGTTGGAGTGCCCC‑C‑TATTCAGGTGGCTAAATTTAGCAAATCAATa                                                                                                      <  2:4100464/140‑1 (MQ=255)

                                    gctgctTGGTGAGATCAGGAAGAGGTTACCGAAACGATGGACATCATCCCCCATTTTGTGGACTGCCCCCCCGGTAGACGATCCTGCCCTATAGTTGGAGTGCCCC‑C‑TATTCAGGTGGCTAAATTTAGCAAATCAATaca                                                                                                    <  1:1493344/140‑1 (MQ=255)

                                     ctgctTGGTGATATCAGGTAGAGGTTACCGAAAAGATGGTCATCATCCCCCATTTTGTGGACGGCCCCCCCGGTAGACGATCCTGCCGGATAGTTTGAGTGCCCC‑C‑TATTCATTTGGCTAAATTTAGCTAATCAATacac                                                                                                   <  2:8857499/140‑1 (MQ=255)

                                     ctgctTGGTGAGATCAGGAAGAGGTTACCGAAACGATGGACATCATCCCCCATTTTGTGGACTGCCCCCCCGGTAGACGATCCTGCCCTATCGTTGGAGTGCCCC‑C‑TATTCAGGTGGCTAAATTTAGCAAATCAATacac                                                                                                   <  2:7942892/140‑1 (MQ=255)

                                     ctgctTGGTGAGATCAGGAAGAGGTTACCGAAACGATGGACATCATCCCCCATTTTGTGGACTGCCCCCCCGGTAGACGATCCTGCCCTATAGTTGGAGTGCCCC‑C‑TATTCAGGTGGCTAATTTTAGCTAATCATTacag                                                                                                   >  1:7436707/1‑139 (MQ=255)

                                     ctgctTGGTGAGATCAGGAAGAGGTTACCGAAACGATGGACATCATCCCCCATTTTGTGGACTGCCCCCCCGGTAGACGATCCTGCCCTATAGTTGGAGTGCCCC‑C‑TATTCAGGTGGCTAAATTTAGCAAATCAATacac                                                                                                   >  2:2398062/1‑140 (MQ=255)

                                     ctgctTGGTGAGATCAGGAAGAGGTTACCGAAACGATGGACATCATCCCCCATTTTGTGGACTGCCCCCCCGGTAGACGATCCTGCCCTATAGTTGGAGTGCCCC‑C‑TATTCAGGTGGCTAAATTTAGCAAATCAATa                                                                                                      >  1:9355872/1‑137 (MQ=255)

                                     ctgctTGGTGAGATCAGGAAGAGGTTACCGAAACGATGGACATCATCCCCCAGTTTGTGGACTGCCCCCCCGGTAGACGATCCTGCCCTTTAGTTGGAGTGCCCC‑C‑TAGTCAGGGGGCTAAATTTAGCAAATCAAGacac                                                                                                   >  2:3815452/1‑140 (MQ=255)

                                          tGGTGAGATCAGGAAGAGGTTACCGAAACGATGGACATCATCCCCCATTTTGTGGACTGCCCCCCCGGTAGACGATCCTGCCCTATAGTTGGAGTGCCCC‑C‑TATTCCGGTGGCTAAATTTAGCAAATCAATACACTTCAg                                                                                              <  1:72670/140‑1 (MQ=255)

                                          tGGTGAGATCAGGAAGAGGTTACCGAAACGATGGACATCATCCCCCATTTTGTGGACTGCCCCCCCGGTAGACGATCCTGCCCTATAGTTGGAGTGCCCC‑C‑TATTCAGGTGGCTAAATTTAGCAAATCAATACACTTCAg                                                                                              <  1:2185070/140‑1 (MQ=255)

                                           ggTGAGATCAGGAAGAGGTTACCGAAACGATGGACATCATCCCCCATTTTGTGGACTGCCCCCCCGGGAGACGATACTGACCTCTAGTTGGAGTGCCCC‑C‑TATTCAGGTGGCTAAATTTAGCAAATCAATACACTTCAgg                                                                                             >  2:9377246/1‑140 (MQ=255)

                                            gttagttCAGGAAGAGGTTACCGAAACGATGGACATCATCCCACATTTTGTGGACTGCCCCCCCGGTAGACGATCCTGCCCTATAGTTGGAGTGCCCC‑C‑TATTCAGGTGGCTAAATTTAGCAAATCAATACACTTCAggg                                                                                            <  2:1807696/134‑1 (MQ=255)

                                            gTGAGATCAGGAAGAGGTTACCGAAACGATGGACATCATCCCCCTTTTTGTGGACTGCCCCCCCGGTAGACGATCCTGCCCTATAGTTGGAGTGCCCC‑C‑TATTCAGGTGGCTAAATTTAGCAAATCAATACACTTCAggg                                                                                            <  1:1239130/140‑1 (MQ=255)

                                            gTGAGATCAGGAAGAGGTTACCGAAACGATGGACATCATCCCCCATTTTGTGGACTGCCCCCCCGGTAGACGATCCTGCCCTATAGTTGGAGTGCCCC‑C‑TATTCAGGTGGCTAAATTTAGCAAATCAATACACTTCAggg                                                                                            <  2:4648671/140‑1 (MQ=255)

                                             tGAGATCAGGAAGAGGTTACCGAAACGATGGACATCATCCCCCATTTTGTGGACTGCCCCCCCGGTAGACGATCCTGCCCTATAGTTGGAGTGCCCC‑C‑TATTCAGGTGGCTAAATTTAGCAAATCAATACACTTCAgggg                                                                                           <  1:4667533/140‑1 (MQ=255)

                                             tGAGATCAGGAAGAGGTTACCGAAACGATGGACATCATCCCCCATTTTGTGGACTGCCCCCCCGGTAGACGATCCTGCCCTATAGGTGGAGTGCCCC‑C‑TATTCAGGTGGCTAAATTTAGCAAATCAATACACTTCAgggg                                                                                           >  1:4519147/1‑140 (MQ=255)

                                               agaTCAGGAAGAGGTTACCGAAACGATGGACATCATCCCCCATTTTGTGGACTGCCCCCCCGGTAGACGATCCTGCCCTATAGTTGGAGTGCCCC‑C‑TATTCAGGTGGCTAAATTTAGCAAATCAATACACTTCAgggggg                                                                                         <  1:3179287/140‑1 (MQ=255)

                                               agaTCAGGAAGAGGTTACCGAAACGATGGACATCATCCCCCATTTTGTGGACTGCCCCCCCGGTAGACGATCCTGCCCTATAGTTGGAGTGCCCC‑C‑TATTCAGGTGGCTAAATTTAGCAAATCAATACACTTCAgggggg                                                                                         <  1:4322133/140‑1 (MQ=255)

                                               agaTCAGGAAGAGGTTACCGAAACGATGGACATCATCCCCCATTTTGTGGACTGCCCCCCCGGTAGACGATCCTGCCCTATAGTTGGAGTGCCCC‑C‑TATTCAGGTGGCTAAATTTAGCAAATCAATACACTTCAgggggg                                                                                         <  1:3893926/140‑1 (MQ=255)

                                                 aTCCGGAAGAGGTGACCGACACGATGGACATCATCCCCCATTTTGTGGACTGCCCCCCCGGTAGACGATCCTGCCCTATAGTTGGAGTGCCCC‑C‑TATTCAGGTGGCTAAATTTAGCAAATCAATACACTTCAGGGGGGta                                                                                       <  2:8244060/140‑1 (MQ=255)

                                                 aTCAGGAAGAGGTTACCGACACGATGGACATCATCCCCCATTTTGTGGACTGCCCCCCCTGTAGACGATCCTGCCCTATAGTTTGAGTGCCCC‑C‑TATTCAGGTGGCTAAATTTAGCAAATCAATACACTTCAGGGGGGta                                                                                       <  2:9249384/140‑1 (MQ=255)

                                                 aTCAGGAAGAGGTTACCGAAACGATGGACATCATCCCCCATTTTGTGGACTGCCCCCCCGGTAGACGATCCTGCCCTATAGTTGGAGTGCCCC‑C‑TATTCAGGTGGCTAAATTTAGCAAATCAATACACTTCAGGGGGGta                                                                                       <  1:6122462/140‑1 (MQ=255)

                                                 aTCAGGAAGAGGTTACCGAAACGATGGACATCATCCCCCATTTTGTGGACTGCCCCCCCGGTAGACGATCCTGCCCTATAGTTGGAGTGCCCC‑C‑TATTCAGGTGGCTAAATTTAGCAAATCAATACACTTCAGGGGGGta                                                                                       >  2:48040/1‑140 (MQ=255)

                                                 aTCAGGAAGAGGTTACCGAAACGATGGACATCATCCCCCATTTTGTGGACTGCCCCCCCGGTAGACGATCCTGCCCTATAGTTGGAGTGCCCC‑C‑TATTCAGGTGGCTAAATTTAGCAAATCAATACACTTCAGGGGGGta                                                                                       <  1:981168/140‑1 (MQ=255)

                                                 aTCAGGAAGAGGTTACCGAAACGATGGACATCATCCCCCATTTTGTGGACTGCCCCCCCGGTAGACGATCCTGCCCTATAGTTGGAGTGCCCC‑C‑TATTCAGGTGGCTAAATTTAGCAAATCAATACACTTCAGGGGGGta                                                                                       <  2:5730485/140‑1 (MQ=255)

                                                 aTCAGGAAGAGGTTACCGAAACGATGGACATCATCCCCCATTTTGTGGACTGCCCCCCCGGTAGACGATCCTGCCCTAGAGTTGGAGTGCCCC‑C‑TATTCAGGTGGCTAAATTTAGCAAATCAATACACTTCAGGGGGGta                                                                                       >  1:1987524/1‑140 (MQ=255)

                                                  tCAGGACGAGCTTACCGTAACTAAGGACATCATCCCCCATTTTGTGGACTGCCCCCACGGTAGACGATCCTTCCCTATAGTTGGAGTG‑CCC‑CATATTCAGGTGTCTAACTGTAGCAAATCAATACACTACAGGGGGGtat                                                                                      <  2:7496589/140‑1 (MQ=255)

                                                     ggAAGAGGTTACCGAAACGATGGACATCATCCCCCATTTTGTGGACTGCCCCCCCGGTAGACGATCCTGCCCTATAGTTGGAGTGCCCC‑C‑TATTCAGGTGGCTAAATTTAGCAAATCAATACACTTCAGGGGGGtattat                                                                                   <  1:5266407/140‑1 (MQ=255)

                                                           ggTTACCGAAACGATGGACATCATCCCCCATTTTGTGGACTGCCCCCCCGGTAGACGATCCTGCCCTATAGTTGGAGTGCCCC‑C‑TATTCAGGTGGCTAAATTTAGCAAATCAATACACTTCAgggggg                                                                                         <  2:5800185/128‑1 (MQ=255)

                                                           ggTTACCGAAACGATGGACATCATCCCCCATTTTGTGGACTGCCCCCCCGGTAGACGATCCTGCCCGATAGTTGGAGTGCCCC‑C‑GATTCAGGTGGCTAAATTTAGCAAATCAATACACTTCAGGGGGGTATTATTGTagt                                                                             >  2:7694208/1‑139 (MQ=255)

                                                             ttACCGAAACGATGGACATCATCCCCCATTTTGTGGACTGCCCCCCCGGTAGACGATCCTGCCCTATAGTTGGAGTGCCCC‑C‑TATTCAGGTGGCTAAATTTAGCAAATCAATACACTTCAGGGGGGTATTATTGTAGAGt                                                                           <  1:1577238/140‑1 (MQ=255)

                                                             ttACCGAAACGATGGACATCATCCCCCATTTTGTGGACTGCCCCCCCGGTAGACGATCCTGCCCTATAGTTGGAGTGCCCC‑C‑TATTCAGGTGGCTAAATTTAGCAAATCAATACACTTCAGGGGGGTATTATTGTAGAGt                                                                           <  2:2162288/140‑1 (MQ=255)

                                                             ttACCGAAACGATGGACATCATCCCCCATTTTGTGGACTGCCCCCCCGGTAGACGATCCTGCCCTATAGTTGGAGTGCCCC‑C‑TATTCAGGTGGCTAAATTTAGCAAATCAATACACTTCAGGGGGGTATTATTGTAGAGt                                                                           <  1:2695537/140‑1 (MQ=255)

                                                              tACCGAAACGATGGACATCATCCCCCATTTTGTGGACTGCCCCCCCGGTAGACGATCCTGCCCTATAGTTGGAGTGCCCC‑C‑TATTCAGGTGGCTATATTTAGCAAATCAATACACTTCAGGGGGGTTTTATAGTAGAGtt                                                                          <  1:6308545/140‑1 (MQ=255)

                                                                 cGAAACGATGGACATCATCCCCCATTTTGTGGACTGCCCCCCCGGTAGACGATCCTGCCCTATAGTTGGAGTGCCCC‑C‑TATTCAGGTGGCTAAATTTAGCAAATCAATACACTTCAGGGGGGTATTATTGTAGAGTTTcc                                                                       <  2:5227966/140‑1 (MQ=255)

                                                                  gAAACGATGGACATCATCCCCCATTGTGTGGACTGCCCCCCCGGTAGACGATCCTGCCCTATAGTTGGAGTGCCCC‑C‑TATTCAGGTGGCTAAATTTAGCAAATCAATACACTTCAGGGGGGTATTATTGTAGAGTTTccc                                                                      <  1:2029476/140‑1 (MQ=255)

                                                                       gATGGACATCATCCCCCATTTTGTGGACTGCCCCCCCGGTAGACGATCCTGCCCTGTAGTTGGAGTGCCCC‑C‑TATTCAGGTGGCTAAATTTAGCAAATCAATACACTTCAGGGGGGTATTATTGTAGAGTTTCCCCatat                                                                 >  1:1339976/1‑140 (MQ=255)

                                                                         ttgACATCAACCCCCAGGGTGTGGACTGCCCCCCCGGTAGAAGATCCTGCCCTAGAGTTGGAGTGCCCC‑C‑TAGTCAGGGGGCTAAATTTAGCCCATCACTACACGTCAGGGGGGTATTATTGTAGAGATTCCCCATCTGt                                                               <  2:8392733/138‑1 (MQ=255)

                                                                          ggACATCATCCCCCATTTTGTGGACTGCCCCCCCGGTAGACGATCCTGCCCTATAGTTGGAGTGCCCC‑C‑TATTCAGGTGGCTAAATTTAGCAAATCAATACACTTCAGGGGGGTATTATTGTAGAGTTTCCCCATATGtt                                                              <  1:3004851/140‑1 (MQ=255)

                                                                              atcatcCCCCATTTTGTGGACTGCCCCCCCGGTAGACGATCCTGCCCTATAGTTGGAGTGCCCC‑C‑TATTCAGGTGGCTAAATTTAGCAAATCAATACACTTCAGGGGGGTATTATTGTAGAGTTTCCCCATATGTTTCTa                                                          <  2:7106563/140‑1 (MQ=255)

                                                                              atcatcCCCCATTTTGTGGACTGCCCCCCCGGTAGACGATCCTGCCCTATAGTTGGAGTGCCCC‑C‑GATTCAGGTGGCTAAATTTAGCAAATCAATACACTTCAGGGGGGTATTATTGTAGAGTTTCCCCATATGTTTCTa                                                          >  2:7856734/1‑140 (MQ=255)

                                                                               tcatcCCCCATTTTGTGGACTGCCCCCCCGGTAGACGATCCTGCCCTATAGTTGGAGTGCCCC‑C‑TATTCAGGTGGCTAAATTTAGCAAATCAATACACTTCAGGGGGGTATTATTGTAGAGTTTCCCCATATGTTTCTAt                                                         <  1:6740140/140‑1 (MQ=255)

                                                                                catcCCCCATTTTGTGGACTGCCCCCCCGGTAGACGATCCTGCCCTATAGTTGGAGTGCCCC‑C‑TATTCAGGTGGCTAAATTTAGCAAATCAATACACTTCAGGGGGGTATTATTGTAGAGTTTCCCCATATGTTTCTATg                                                        >  2:5973220/1‑140 (MQ=255)

                                                                                catcCCCCATTTTGTGGACTGCCCCCCCGGTAGACGATCCTGCCCTATAGTTGGAGTG‑CCCAC‑TATTCAGGTGGCTAAATTTAGCAAATCAATACACTTCAGGGGGGTATTATTGTAGAGTTTCCCCATATGTTTCTATg                                                        <  2:7289818/140‑1 (MQ=255)

                                                                                 atcCCCCATTTTGTGGACTGCCCCCCCGGTAGACGATCCTGCCCTATAGTTGGAGTGCCCC‑C‑TATTCAGGTGGCTAAATTTAGCAAATCAATACACTTCAGGGGGGTATTATTGTAGAGTTTCCCCATATGTTTCTATgg                                                       <  2:1495831/140‑1 (MQ=255)

                                                                                     cccATTTTGTGGACTGCCCCCCCGGTAGACGATCCTGCCCTATAGTTGGAGTGCCCC‑C‑TCGTCAGGTGGCTAAATTTAGCAAATCAATACACTTCAGGGGGGTATTATTGTAGAGGTTCCCCATATGTTTCTATGGGATc                                                   <  1:240752/140‑1 (MQ=255)

                                                                                         ttttGTGGACTGCCCCCCCGGTAGACGATCCTGCCCTATAGTTGGAGTGCCCC‑C‑GATTCAGGTGGCTAAATTTAGCAACTCAATACACTTCAGGGGGGTATTATTGTAGAGTTTCCCCATATGTTTCTATGGGATCCAgg                                               <  2:6734039/140‑1 (MQ=255)

                                                                                             gtgGACTGCCCCCCCGGTAGACGATCCTGCCCTATAGTTGGAGTGCCCC‑C‑TATTCAGGTGGCTAAATTTAGCAAATCAATACACTTCAGGGGGGTATTATTGTAGAGTTTCCCCATATGTTTCTATGGGATCCAGGAAAt                                           <  1:6052386/140‑1 (MQ=255)

                                                                                               ggACTGCCCCCCCGGTAGACGATCCTGCCCTATAGTTGGAGTGCCCC‑C‑TATTCAGGTGGCTAAATTTAGCAAATCAATACACTTCAGGGGGGTATTATTGTAGAGTTTCCCCATATGTTTCTATGGGATCCAGGAAATGa                                         <  2:3731427/140‑1 (MQ=255)

                                                                                                 aCTGCCCCCCCGGTAGACGATCCTGCCCTATAGTTGGAGTGCCCC‑C‑TCTTCAGGGGGCTAAATTTAGCAAATCAATACACTTCAGGGGGGTATTATTGTAGAGTTTCCCCATATGTTTCTATGGGATCCAGGAAATGACa                                       <  2:4266008/140‑1 (MQ=255)

                                                                                                   tGCCCCCCCGGTAGACGATCCTGCCCTATAGTTGGAGTGCCCC‑C‑TATTCAGGTGGCTAAATTTAGCAAATCAATACACtt                                                                                                 >  2:6259677/1‑80 (MQ=255)

                                                                                                   tGCCCCCCCGGTAGACGATCCTGCCCTATAGTTGGAGTGCCCC‑C‑TATTCAGGTGGCTAAATTTAGCAAATCAATACACTTCAGGGGGGTATTATTGTAGAGTTTCCCCATATGTTTCTATGGGATCCAGGAAATGACAAt                                     >  2:5646360/1‑140 (MQ=255)

                                                                                                   tGCCCCCCCGGTAGACGATCCTGCCCTATAGTTGGAGTGCCCC‑C‑TATTCAGGTGGCTAAATTTAGCAAATCAATACACTTCAGGGGGGTATTATTGTAGAGTTTCCCCATATGTTTCTATGGGATCCAGGAAATGACAAt                                     >  1:104897/1‑140 (MQ=255)

                                                                                                     cccccccGGTAGACGATCCTGCCCTATAGTTGGAGTGCCCC‑C‑TATTCAGGTGGCTAAATTTAGCAAATCAATACACTTCAGGGGGGTATTATTGTAGAGTTTCCCCATATGt                                                               >  1:1425128/1‑112 (MQ=255)

                                                                                                     cccccccGGTAGACGATCCTGCCCTATAGTTGGAGTGCCCC‑C‑TATTCAGGTGGCTAAATTTAGCAAATCAATACACTTCAGGGGGGTATTATTGTAGAGTTTCCCCATATGTTTCTATGGGATCCAGGAAATGACAATCa                                   >  2:4441510/1‑140 (MQ=255)

                                                                                                     cccccccGGTAGACGATCCTGCCCTATAGTTGGAGTGCCCC‑C‑TATTCAGGTGGCTAAATTTAGCAAATCAATACACTTCAGGGGGGTATTATTGTAGAGTTTCCCCATATGTTTCTATGGGATCCAGGAAATGACAATCa                                   >  2:8045809/1‑140 (MQ=255)

                                                                                                             gTAGACGATCCTGCCCTATAGTTGGAGTGCCCC‑C‑TATTCAGGTGGCTAAATTTAGCAAATCAATACACTTCAGGGGGGTATTATTGTAGAGTTTCCCCATATGTTTCTATGGGATCCAGGAAATGACAATCAGGAGTTAc                           >  1:1008377/1‑140 (MQ=255)

                                                                                                               agaagaTCCTGCCCTATAGTTGGAGTG‑CCC‑CATATTCAGGTGGATAAATTTAGCAAATCAATACACTTCAGGGGGGTATTATTGTAGAGTTTCCCCATATGTTTCTATGGGATCCAGGAAATGACAATCAGGAGTTACaa                         <  1:8052703/140‑1 (MQ=255)

                                                                                                               aGACGATCCTGCCCTATAGTTGGAGTGCCCC‑C‑TATTCAGGTGGCTATATTTAGCAAATCAATACACTTCAGGGGGGTATTATTGTAGAGTTTCCCCATATGCTTCTATGGGATCCAGGAAATGACAATCAGGAGTTCCaa                         <  2:2988718/140‑1 (MQ=255)

                                                                                                               aGACGATCCTGCCCTATAGTTGGAGTGCCCC‑C‑TATTCAGGTGGCTAAATTTAGCAAATCAATACACTTCAGGGGGGTATTATTGTAGAGTTTCCCCATATGTTTCTATGGGATCCAGGAAATGACAATCAGGAGTTACaa                         <  1:2865381/140‑1 (MQ=255)

                                                                                                               aGACGATCCTGCCCTAGAGTTGGAGTGCCCC‑C‑TATTCAGGTGGCTAAATTTAGCAAATCAATACACTTCAGGGGGGTATTATTGTAGAGTTTCCCCATATGTTTCTATGGGATCCAGGAAATGACAATCAGGAGTTACaa                         <  2:8491936/140‑1 (MQ=255)

                                                                                                                  cGATCCTGCCCTATAGTTGGAGTGCCCC‑C‑TATTCAGGTGGCTAAATTTAGCAAATCAATACACTTCAGGGGGGTATTATTGTAGAGTTTCCCCATATGTTTCTATGGGATCCAGGAAATGACAATCAGGAGTTACaaaaa                      <  2:3908299/140‑1 (MQ=255)

                                                                                                                      ccTGCCCTATAGTTGGAGTGCCCC‑C‑TATTCAGGTGGCTAATTTTAGCAAATCAATACACTTCAGGGGGGTATTATTGTAGAGTTTCCCCATATGTTTCTAGGGGATCCAGGAAATGACAATCAGGAGTTACAAAAACTTa                  >  2:2644926/1‑140 (MQ=255)

                                                                                                                      ccTGCCCTATAGTTGGAGTGCCCC‑C‑TATTCAGGTGGCTAAATTTAGCAAATCAATACACTTCAGGGGGGTATTATTGTAGAGTTTCCCCATATGTTTCTATGGGATCCAGGAAATGACAATCAGGAGTTACAAAAACTTa                  >  1:6551669/1‑140 (MQ=255)

                                                                                                                          cccTATTGTTGGAGTGCCCC‑C‑TATTCAGGTGGCTAATGTTAGCAAATCAATACACTTCAGGGGGGTATTATTGTAGAGTTTCCCCATATGTTTCTATGGGATCCAGGAAATGACCATCAGGAGTTACAAAAACTTAAATc              <  2:5125841/140‑1 (MQ=255)

                                                                                                                              ataGTTGGAGTG‑CCCAC‑TATTCAGGTGGCTAAATTGAGCAAATCAATACACTTCAGGGGGGTATTATTGTAGAGTTTCCCCATATGTTTCTATGGGATCCAGGAAATGACAATCAGGAGTTACAAAAACTTAAATCTGGt          <  2:1371134/140‑1 (MQ=255)

                                                                                                                               taGTTGGAGTGCCCC‑C‑TATTCAGGTGGCTAAATTTCGCAAATCAATACACATCAGGGGGGTATTATTGTAGAGTTTCCCCATATGTTTCTCTGGGATCCAGGAAATGACAATCAGGAGTTACAAAAACTTAAATCTGGTc         <  2:2709137/140‑1 (MQ=255)

                                                                                                                               taGTTGGAGTGCCCC‑C‑TATTCAGGTGGCTAAATTTAGCAAATCAATACACTTCAGGGGGGTATTATTGTAGAGTTTCCCCATATGTTTCTATg                                                        >  1:7967533/1‑93 (MQ=255)

                                                                                                                               taGTTGGAGTGCCCC‑C‑TATTCAGGTGGCTAAATTTAGCAAATCAATACACTTCAGGGGGGTATTATTGTAGAGTTTCCCCATATGTTTCTATGGGATCCAGGAAATGACAATCAGGAGTTACAAAAACTTAAATCTGGt          >  1:4173178/1‑139 (MQ=255)

                                                                                                                               taGTTGGAGTGCCCC‑C‑TATTCAGGTGGCTAAATTTAGCAAATCAATACACTTCAGGGGGGTATTATTGTAGAGTTTCCCCATATGTTTCTATGGGATCCAGGAAATGACAATCAGGAGTTACAAAAACTTAAATCTGGTc         >  2:8133712/1‑140 (MQ=255)

                                                                                                                               taGTTGGAGTGCCCC‑C‑TATTCAGGTGGCTAAATTTAGCAAATCAATACACTTCAGGGGGGTATTATTGTAGAGTTTCCCCATATGTTTCTATGGGATCCAGGAAATGACAATCAGGAGTTACAAAAACTTAAATCTGGTc         >  1:8928444/1‑140 (MQ=255)

                                                                                                                               taGTTGGAGTGCCCC‑C‑TATTCAGGTGGCTAAATTTAGCAAATCAATACACTTCAGGGGGGTATTATTGTAGAGTTTCCCCATATGTTTCTATGGGATCCAGGAAATGACAATCAGGAGTTACAAAAACTTAAATCTGGTc         <  1:2929716/140‑1 (MQ=255)

                                                                                                                               taGTTGGAGTGCCCC‑C‑TATTCAGGTGGCAAAATTTAGCAAAACAAGACACTTCAGGGGGGTATTTGTGTAGAGTTTCCCCATATGTTTCTATGGGATCCAGGAAATGACAATCAGGAGTTACAAAAACTTAAATCTGGTc         <  2:4826098/140‑1 (MQ=255)

                                                                                                                                    ggAGTGCCCC‑C‑TATTCAGGTGGCTAAATTTAGCAAATCAATACACTTCAGGGGGGTATTATTGTAGAGTTTCCCCATATGTTTCTATGGGATCCAGGAAATGACAATCAGGAGTTACAAAAACTTAAATCTGGTCAGGGc    >  1:8438286/1‑140 (MQ=255)

                                                                                                                                    ggAGTGCCCC‑C‑TATTCAGGTGGCTAAATTTAGCAAATCAATACACTTCAGGGGGGTATTATTGTAGAGTTTCCCCATATGTTTCTATGGGATCCAGGAAATGACAATCAGGAGTTACAAAAACTTAAATCTGGTCAGGGc    >  2:6116945/1‑140 (MQ=255)

                                                                                                                                    ggAGTGCCCC‑C‑TATTCAGGTGGCTAAATTTAGCAAATCAATACACTTCAGGGGGGTATTATTGTAGAGTTTCCCCATATGTTTCTATGGGATCCAGGAAATGACAATCAGGAGTTACAAAAACTTAAATCTGGTCAGGGc    >  2:3582354/1‑140 (MQ=255)

                                                                                                                                     gAGTGCCCC‑C‑TATTCAGGTGGCTAAATTTAGCAAATCAATACACTTCAGGGGGGTATTATTGTAGAGTTTCCCCATATGTTTCTATGGGATCCAGGAAATGACAATCAGGAGTTACAAAAACTTAAATCTGGTCAGGGCa   <  1:5485888/140‑1 (MQ=255)

                                                                                                                                     gAGTGCCCC‑C‑TATTCAGGTGGCTAAATTTAGCAAATCAATACACTTCAGGCGGGTATTATTGTAGAGTTTCCCCATATGTTTCTATGGGATCCAGGAAATGACAATCAGGAGTTACAAAAACTTAAATCTGGTCAGGGCa   <  1:758093/140‑1 (MQ=255)

CTTGTCTGCTGGCGAATAATTACTAAGTATCGAGTTGCTGCTTGGTGAGATCAGGAAGAGGTTACCGAAACGATGGACATCATCCCCCATTTTGTGGACTGCCCCCCCGGTAGACGATCCTGCCCTATAGTTGGAGTG‑CCC‑C‑TATTCAGGTGGCTAAATTTAGCAAATCAATACACTTCAGGGGGGTATTATTGTAGAGTTTCCCCATATGTTTCTATGGGATCCAGGAAATGACAATCAGGAGTTACAAAAACTTAAATCTGGTCAGGGCAA  >  NZ_CP025268/2769711‑2769983

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`