BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP025268	3,239,247	(T)_6→7	intergenic (+264/‑18)	ygjR → / → alx	Gfo/Idh/MocA family oxidoreductase/TerC family membrane protein Alx

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP025268	3,239,241	1	.	T	94.9%	1550.3 / 62.9	438	intergenic (+258/‑24)	ygjR/alx	Gfo/Idh/MocA family oxidoreductase/TerC family membrane protein Alx
Reads supporting (aligned to +/- strand): ref base . (14/8); new base T (175/238); total (189/249)
Fisher's exact test for biased strand distribution p-value = 7.53e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 516 total aligned reads displayed.

TGTGTGAAAAAACACATCCGGTCACCGGGCAACCCGAAAGGAATACGCAGACGTATTCCTTTTTTGTTGTAAGTGAGACCTTGCCGGAAGGCGAGGTCTATGCATAAAAAGCAGCGGCTGACGTCTTCCGACGTTGGCCG‑TTT‑T‑T‑TATGTGTAAGGAACTTCTATGAATACTGTCGGCACGCCGTTGCTATGGGGCGGATTCGCTGTTGTTGTCGCCATTATGCTGGCTATCGACCTGTTGTTGCAGGGGCGTCGTGGGGCACATGCCATGACCA  >  NZ_CP025268/3239102‑3239376

tgtgtgAAAAAACACATCCGGTCACCGGGCAACCCGAAAGGAATACGCAGACGTATTCCTTTTTTGTTGTAAGTGAGACCTTGCCGGAAGGCGAGGTCTATGCATAAAAAGCAGCGGCTGACGTCTTCCGACGTTGGCCg                                                                                                                                             >  2:7034532/1‑140 (MQ=255)

  tgtgAAAAAACACATCCGGTCACCGGGCAACCCGAAAGGAATACGCAGACGTATTCCTTTTTTGTTGTAAGTGAGACCTTGCCGGAAGGCGAGGTCTATGCATAAAAAGCAGCGGCTGACGTCTTCCGACGTTGGCCG‑tt                                                                                                                                          >  2:9403331/1‑140 (MQ=255)

  tgtgAAAAAACACATCCGGTCACCGGGCAACCCGAAAGGAATACGCAGACGTATTCCTTTTTTGTTGTAAGTGAGACCTTGCCGGAAGGCGAGGTCTATGCATAAAAAGCAGCGGCTGACGTCTTCCGACGTTGGCCG‑tt                                                                                                                                          >  1:5265313/1‑140 (MQ=255)

  tgtgAAAAAACACATCCGGTCACCGGGCAACCCGAAAGGAATACGCAGACGTATTCCTTTTTTGTTGTAAGTGAGACCTTGCCGGAAGGCGAGGTCTATGCATAAAAAGCAGCGGCTGACGTCTTCCGACGTTGGCCG‑tt                                                                                                                                          >  2:8784301/1‑140 (MQ=255)

  tgtgAAAAAACACATCCGGTCACCGGGCAACCCGAAAGGAATACGCAGACGTATTCCTTTTTTGTTGTAAGTGAGACCTTGCCGGAAGGCGAGGTCTATGCATAAAAAGCAGCGGCTGACGTCTTCCGACGTTGGCCG‑tt                                                                                                                                          >  1:7807118/1‑140 (MQ=255)

  tgtgAAAAAACACATCCGGTCACCGGGCAACCCGAAAGGAATACGCAGACGTATTCCTTTTTTGTTGTAAGTGAGACCTTGCCGGAAGGCGAGGTCTATGCATAAAAAGCAGCGGCTGACGTCTTCCGACGTTGGCCG‑tt                                                                                                                                          >  2:8671473/1‑140 (MQ=255)

  tgtgAAAAAACACATCCGGTCACCGGGCAACCCGAAAGGAATACGCAGACGTATTCCTTTTTTGTTGTAAGTGAGACCTTGCCGGAAGGCGAGGTCTATGCATAAAAAGCAGCGGCTGACGTCTTCCGACGTTGGCCG‑tt                                                                                                                                          >  1:8338836/1‑140 (MQ=255)

  tgtgAAAAAACACATCCGGTCACCGGGCAACCCGAAAGGAATACGCAGACGTATTCCTTTTTTGTTGTAAGTGAGACCTTGCCGGAAGGCGAGGTCTATGCATAAAAAGCAGCGGCTGACGTCTTCCGACGTTGGCCG‑tt                                                                                                                                          >  2:4573408/1‑140 (MQ=255)

  tgtgAAAAAACACATCCGGTCACCGGGCAACCCGAAAGGAATACGCAGACGTATTCCTTGTTTGTTGTAAGTGAGACCTTGCCGGAAGGCGAGGTCTATGCATAAAAAGCAGCGGCTGACGTCTTCCGACGTTGGCCG‑tt                                                                                                                                          >  1:6116808/1‑140 (MQ=255)

  tgggAAAAAACACAACAGGTCACAGGGCAACCCGAAAGGAATACGCAGACGTATTCCTTTTTTGTTGTAAGTGAGACCTTGCAGGAAGGCGAGGTCTATGCATAAAAAGCAGCTGCTGACGTCTTCCTACGTTGGCAG‑tt                                                                                                                                          >  2:4921779/4‑140 (MQ=255)

    tgAAAAAACACATCCGGTCACCGGGCAACCCGAAAGGAATACGCAGACGTATTCCTTTTTTGTTGTAAGTGAGACCTTGCCGGAAGGCGAGGTCTATGCATAAAAAGCAGCGGCTGACGTCTTCCGACGTTGGCCG‑ttt‑t                                                                                                                                       >  1:4948552/1‑140 (MQ=255)

       aaaaaCACATCCGGTCACCGGGCAACCCGAAAGGAATACGCAGACGTATTCCTTTTTTGTTGTAAGTGAGACCTTGCCGGAAGGCGAGGTCTATGCATAAAAAGCAGCGGCTGACGTCTTCCGACGTTGGCCG‑ttt‑t‑t‑tt                                                                                                                                  <  1:150004/140‑2 (MQ=255)

       aaaaaCACATCCGGTCACCGGGCAACCCGAAAGGAATACGCAGACGTATTCCATTTTTGTTGTAAGTGAGACCTTGCCGGAAGGCGAGGTCTATGCATAAAAAGCAGCGGCTGACGTCTTCCGACGTTGGCCG‑ttt‑t‑t‑tt                                                                                                                                  <  2:508434/140‑2 (MQ=255)

         aaaCACATCCGGTCACCGGGCAACCCGAAAGGAATACGCAGACGTATTCCTTTTTTGTTGTAAGTGAGACCTTGCCGGAAGGCGAGGTCTATGCATAAAAAGCAGCGGCTGACGTCTTCCGACGTTGGCCG‑ttt‑t‑t‑ttat                                                                                                                                <  2:4278745/140‑4 (MQ=255)

         aaaCACATCCGGTCACCGGGCAACCCGAAAGGAATACGCAGACGTATTCCTTTTTTGTTGTAAGTGAGACCTTGCCGGAAGGCGAGGTCTATGCATAAAAAGCAGCGGCTGACGTCTTCCGACGTTGGCCG‑ttt‑t‑t‑ttat                                                                                                                                <  2:1231196/140‑4 (MQ=255)

         aaaCACATCCGGTCACCGGGCAACCCGAAAGGAATACGCAGACGTATTCCTTTTTTGTTGTAAGTGAGACCTTGCCGGAAGGCGAGGTCTATGCATAAAAAGCAGCGGCTGACGTCTTCCGACGTTGGCCG‑ttt‑t‑t‑ttat                                                                                                                                <  2:5512562/140‑4 (MQ=255)

         aaaCACATCCGGTCACCGGGCAACCCGAAAGGAATACGCAGACGTATTCCTTTTTTGTTGTAAGTGAGACCTTGCCGGAAGGCGAGGTCTATGCATAAAAAGCAGCGGCTGACGTCTTCCGACGTTGGCCG‑ttt‑t‑t‑ttat                                                                                                                                <  1:2064958/140‑4 (MQ=255)

            cacaTCCGGTCACCGGGCAACCCGAAAGGAATACGCAGACGTATTCCTTTTTTGTTGTAAGTGAGACCTTGCCGGAAGGCGAGGTCTATGCATAAAAAGCAGCGGCTGACGTCTTCCGACGTTGGCCGTTTT‑T‑T‑TAtgtg                                                                                                                              >  1:5476638/1‑140 (MQ=255)

            cacaTCCGGTCAACGGGCAACACGAAAGGAATACGCAGACGTATTCCTTTTTTGTTGTAAGTGAGACCTTGCCGGAAGGCGAGGTCTATGCATAAAAAGCAGCGGCTGACGTCTGCCGACGTTGGGCGTTTT‑T‑T‑TAtgtg                                                                                                                              >  1:1647152/1‑140 (MQ=255)

              caTCCGGTCACCGGGCAACCCGAAAGGAATACGCAGACGTATTCCTTTTTTGTTGTAAGTGAGACCTTGCCGGAAGGCGAGGTCTATGCATAAAAAGCAGCGGCTGACGTCTTCCGACGTTGGCCGTTTT‑T‑T‑TATGTGTa                                                                                                                            >  1:1292474/1‑140 (MQ=255)

              caTCCGGTCACCGGGCAACCCGAAAGGAATACGCAGACGTATTCCTTTTTTGTTGTAAGTGAGACCTTGCCGGAAGGCGAGGTCTATGCATAAAAAGCAGCGGCTGACGTCTTCCGACGTTGGCCGTTTT‑T‑T‑TATGTGTa                                                                                                                            >  2:2646922/1‑140 (MQ=255)

                 ccGGTCACCGGGCAACCCGAAAGGAATACGCAGACGTATTCCTTTTTTGTTGTAAGTGAGACCTTGCCGGAAGGCGAGGTCTATGCATAAAAAGCAGCGGCTGACGTCTTCCGACGTTGGCCGTTTT‑T‑T‑TATGTGTAAgg                                                                                                                         <  1:4552272/140‑1 (MQ=255)

                   ggTCACCGGGCAACCCGAAAGGAATACGCAGACGTATTCCTTTTTTGTTGTAAGTGAGACCTTGCCGGAAGGCGAGGTCTATGCATAAAAAGCAGCGGCTGACGTCTTCCGACGTTGGCCGTTTT‑T‑T‑TATGTGTAAGGaa                                                                                                                       <  2:1575432/140‑1 (MQ=255)

                   ggTCACCGGGCAACCCGAAAGGAATACGCAGACGTATTCCTTTTTTGTTGTAAGTGAGACCTTACCGGAAGGCGAGGTCTATGCATAAAAAGCAGCGGCTGACGTCTTCCGACGTTGGCCGTTTT‑T‑T‑TATGTGTAAGGaa                                                                                                                       <  1:9420109/140‑1 (MQ=255)

                     tCACCGGGCAACCCGCAAGGAATACGCAGACGTATTCCTTTTTTGTTGTAAGTGAGACCTTGCCGGAAGGCGAGGTCTATGCATAAAAAGCAGCGGCTGACGTCTTCCGACGTTGGCCGTTTT‑T‑T‑TATGTGTAAGGAACt                                                                                                                     <  2:8328968/140‑1 (MQ=255)

                     tCACCGGGCAACCCGAAAGGAATACGCATACCTATTCCTTTTATGTTGGAAGTGAGACCTAGCCGGAAGGCGAGGTCTATGCAAAAAAAGCAGCGGCTGACGTCTTCCGACGTTGGCCG‑TTTGT‑G‑AATGTGTCAGGAAcg                                                                                                                     >  1:7083092/1‑139 (MQ=255)

                     tCACCGGGCAACCCGAAAGGAATACGCAGACGTATTCCTTTTTTGTTGTAAGTGAGACCTTGCCGGAAGGCGAGGTCTATGCATAAAAAGCAGCGGCTGACGTCTTCCGACGTTGGCCGTTTT‑T‑T‑TATGTGTAAGGAACt                                                                                                                     <  1:1023544/140‑1 (MQ=255)

                     tCACCGGGCAACCCGAAAGGAATACGCAGACGTATTCCTTTTTTGTTGTAAGTGAGACCTTGCCGGAAGGCGAGGTCTATGCATAAAAAGCAGCGGCTGACGTCTTCCGACGTTGGCCGTTTT‑T‑T‑TATGTGTAAGGAACt                                                                                                                     <  1:2333846/140‑1 (MQ=255)

                     tCACCGGGCAACCCGAAAGGAATACGCAGACGTATTCCTTTTTTGTTGTAAGTGAGACCTTGCCGGAAGGCGAGGTCTATGCATAAAAAGCAGCGGCTGACGTCTTCCGACGTTGGCCGTTTT‑T‑T‑TATGTGTAAGGAACt                                                                                                                     <  1:5935361/140‑1 (MQ=255)

                     tCACCGGGCAACCCGAAAGGAATACGCAGACGTATTCCTTTTTTGTTGTAAGTGAGACCTTGCCGGAAGGCGAGGTCTATGCATAAAAAGCAGCGGCTGACGTCTTCCGACGTTGGCCGTTTT‑T‑T‑TATGTGTAAGGAACt                                                                                                                     <  1:3878201/140‑1 (MQ=255)

                         cGGGCAACCCGAAAGGAATACGCAGACGTATTCCTTTTTTGTTGTAAGTGAGACCTTGCCGGAAGGCGAGGTCTATGCATAAAAAGCAGCGGCTGACGTCTTCCGACGTTGGCCGTTTT‑T‑T‑TATGTGTAAGGAACt                                                                                                                     >  1:8484583/1‑136 (MQ=255)

                           ggCAACCCGAAAGGAATACGCAGACGTATTCCTTTTTTGTTGTAAGTGAGACCTTGCCGGAAGGCGAGGTCTATGCATAAAAAGCAGCGGCTGACGTCTTCCGACGTTGGCCGTTTT‑T‑T‑TATGTGTAAGGAACTTCTATg                                                                                                               >  2:3541855/1‑140 (MQ=255)

                            gCAACCCGAAAGGAATACGCAGACGTATTCCTTTTTTGTTGTAAGTGAGACCTTGCCGGAAGGCGAGGTCTATGCATAAAAAGCAGCGGCTGACGTCTTCCGACGTTGGCCGTTTT‑T‑T‑TATGTGTAAGGAACTTCTATGa                                                                                                              <  1:380293/140‑1 (MQ=255)

                              aaCCCGAAAGGAATACGCAGACGTATTCCTTTTTTGTTGTAAGTGAGACCTTGCCGGAAGGCGAGGTCTATGCATAAAAAGCAGCGGCTGACGTCTTCCGACGTTGGCCGTTTT‑T‑T‑TATGTGTAAGGAACTTCTATGAAt                                                                                                            <  2:1677507/140‑1 (MQ=255)

                              aaCCCGAAAGGAATACGCAGACGTATTCCTTTTTTGTTGTAAGTGAGACCTTGCCGGAAGGCGAGGTCTATGCATAAAAAGCAGCGGCTGACGTCTTCCGACGTTGGCCGTTTT‑T‑T‑TATGTGTAAGGAACTTCTATGAAt                                                                                                            <  1:7588418/140‑1 (MQ=255)

                                   gAAAGGAATACGCAGACGTATTCCTTTTTTGTTGTAAGTGAGACCTTGCCGGAAGGCGAGGTCTATGCATAAAAAGCAGCGGCTGACGTCTTCCGACGTTGGCCGTTTT‑T‑T‑TATGTGTAAGGAACTTCTATGAATACTGt                                                                                                       <  2:1112343/140‑1 (MQ=255)

                                    aaaGGAATACGCAGACGTATTCCTTTTTTGTTGTAAGTGAGACCTTGCCGGAAGGCGAGGTCTATGCATAAAAAGCAGCGGCTGACGTCTTCCGACGTTGGCCGTTTT‑T‑T‑TATGTGTAAGGAACTTCTATGAATACTGTc                                                                                                      >  1:5624093/1‑140 (MQ=255)

                                      aGGAATACGCAGACGTATTCCTTTTTTGTTGTAAGTGAGACCGTGCCGGAAGGCGAGGTCTATGCATACAAAGCAGCGGCTGACGTCTTCCGACGTTGGCCGTTTT‑T‑T‑TATGTGTAAGGAACTTCTATGAATACTGTCgg                                                                                                    <  2:2486433/140‑1 (MQ=255)

                                        gAATACGCAGACGTATTCCTTTTTTGTTGTAAGTGAGACCTTGCCGGAAGGCGAGGTCTATGCATAAAAAGCAGCGGCTGACGTCTTCCGACGTTGGCCGTTTT‑T‑T‑TATGTGTAAGGAACTTCTATGAATACTGTCGGCa                                                                                                  <  2:7482690/140‑1 (MQ=255)

                                              gCAGACGTATTCCTTTTTTGTTGTAAGTGAGACCTTGCCGGAAGGCGAGGTCTATGCATAAAAAGCAGCGGCTGACGTCTTCCGACGTTGGCCGTTTT‑T‑T‑TATGTGTAAGGAACTTCTATGAATACTGTCGGCACGCCGt                                                                                            <  2:1478890/140‑1 (MQ=255)

                                                  aCGTATTCCTTTTTTGTTGTAAGTGAGACCTTGCCGGAAGGCGAGGTCTATGCATAAAAAGCAGCGGCTGACGTCTTCCGACGTTGGCCGTTTT‑T‑T‑TATGTGTAAGGAACTTCTATGAATACTGTCGGCACGCCGTTGCt                                                                                        >  2:5701539/1‑140 (MQ=255)

                                                  aCGTATTCCTTTTTTGTTGTAAGTGAGACCTTGCCGGAAGGCGAGGTCTATGCATAAAAAGCAGCGGCTGACGTCTTCCGACGTTGGCCGTTTT‑T‑T‑TATGTGTAAGGAACTTCTATGAATACTGTCGGCACGCCGTTGCt                                                                                        >  1:8546689/1‑140 (MQ=255)

                                                  aCGTATTCCTTTTTTGTTGTAAGTGAGACCTTGCCGGAAGGCGAGGTCTATGCATAAAAAGCAGCGGCTGACGTCTTCCGACGTTGGCCG‑TTT‑T‑T‑TATGTGTAAGGAACTTCTATGACTACTGTCGGCACGCCGTTGCTa                                                                                       >  2:3328940/1‑140 (MQ=255)

                                                   cGTATTCCTTTTTTGTTGTAAGTGAGACCTTGCCGGAAGGCGAGGTCTATGCATAAAAAGCAGCGGCTGACGTCTTCCGACGTTGGCCGTTTT‑T‑T‑TATGTGTAAGGAACTTCTATGAATACTGTCGGCACGCCGTTGCTa                                                                                       >  1:4227021/1‑140 (MQ=255)

                                                   cGTATTCCTTTTTTGTTGTAAGTGAGACCTTGCCGGAAGGCGAGGTCTATGCATAAAAAGCAGCGGCTGACGTCTTCCGACGTTGGCCGTTTT‑T‑T‑TATGTGTAAGGAACTTCTATGAATACTGTCGGCACGCCGTTGCTa                                                                                       >  1:2534951/1‑140 (MQ=255)

                                                        tCCTTTTTTGTTGTAAGTGAGACCTTGCCGGAAGGCGAGGTCTATGCATAAAAAGCAGCGGCTGACGTCTTCCGACGTTGGCCGTTTT‑T‑T‑TATGTGTAAGGAACTTCTATGAATACTGTCGGCACGCCGTTGCTATgggg                                                                                  <  1:8030960/140‑1 (MQ=255)

                                                        tCCTTTTTTGTTGTAAGTGAGACCTTGCCGGAAGGCGAGGTCTATGCATAAAAAGCAGCGGCTGACGTCTTCCGACGTTGGCCGTTTT‑T‑T‑TATGTGTAAGGAACTTCTATGAATACTGTCGGCACGCCGTTGCTATgggg                                                                                  <  1:8677427/140‑1 (MQ=255)

                                                            tttttGTTGTAAGTGAGACCTTGCCGGAAGGCGAGGTCTATGCATAAAAAGCAGCGGCTGACGTCTTCCGACGTTGGCCGTTTT‑T‑T‑TATGTGTAAGGAACTTCTATGAATACTGTCGGCACGCCGTTGCTATGGGGCGGa                                                                              >  1:314025/1‑140 (MQ=255)

                                                            tttttGTTGTAAGTGAGACCTTGCCGGAAGGCGAGGTCTATGCATAAAAAGCAGCGGCTGACGTCTTCCGACGTTGGCCGTTTT‑T‑T‑TATGTGTAAGGAACTTCTATGAATACTGTCGGCACGCCGTTGCTATGGGGCGGa                                                                              <  2:3255567/140‑1 (MQ=255)

                                                                 gttgtAAGTGAGACCTTGCCGGAAGGCGAGGTCTATGCATAAAAAGCAGCGGCTGACGTCTTCCGACGTTGGCCGTTTT‑T‑T‑TATGTGTAAGGAACTTCTATGAATACTGTCGGCACGCCGTTGCTATGGGGCGGATTCGc                                                                         >  2:9059362/1‑140 (MQ=255)

                                                                       aGTGAGACCTTGCCGGAAGGCGAGGTCTATGCATAAAAAGCAGCGGCTGACGTCTTCCGACGTTGGCCGTTTT‑T‑T‑TATGTGTAAGGAACTTCTATGAATACTGTCGGCACGCCGTTGCTATGGGGCGGATTCGCtgttgt                                                                   <  2:8158391/140‑1 (MQ=255)

                                                                          gagaCCTTGCCGGAAGGCGAGGTCTATGCATAAAAAGCAGCGGCTGACGTCTTCCGACGTTGGCCGTTTT‑T‑T‑TATGTGTAAGGAACTTCTATGAATACTGTCGGCACGCCGTTGCTATGGGGCGGATTCGCtgttgttgt                                                                <  2:6594069/140‑1 (MQ=255)

                                                                          gagaCCTTGCCGGAAGGCGAGGTCTATGCATAAAAAGCAGCGGCTGACGTCTTCCGACGTTGGCCGTTTT‑T‑T‑TATGTGTAAGGAACTTCTATGAATACTGTCGGCACGCCGTTGCTATGGGGCGGATTCGCtgttgttgt                                                                <  2:584275/140‑1 (MQ=255)

                                                                          gagaCCTTGCCGGAAGGCGAGGTCTATGCATAAAAAGCAGCGGCTGACGTCTTCCGACGTTGGCCGTTTT‑T‑T‑TATGTGTAAGGAACTTCTATGAATACTGTCGGCACGCCGTTGCTATGGGGCGGATTCGCtgttgttgt                                                                <  1:5035917/140‑1 (MQ=255)

                                                                          gagaCCTTGCCGGAAGGCGAGGTCTATGCATAAAAAGCAGCGGCTGACGTCTTCCGACGTTGGCCGTTTT‑T‑T‑TATGTGTAAGGAACTTCTATGAATACTGTCGGCACGCCGTTGCTATGGGGCGGATTCGCtgttgttgt                                                                <  1:8262630/140‑1 (MQ=255)

                                                                               cTTGCCGGAAGGCGAGGTCTATGCATAAACAGCAGCGGCTGACGTCTTCCGACGTTGGCCGTTTT‑T‑T‑TATGTGTAAGGAACTTCTATGAATACTGTCGGCACGCCGTTGCTATGGGGCGGATTCGCTGTTGTTGTCGCCa                                                           <  1:4311616/140‑1 (MQ=255)

                                                                                 tGCCGGAAGGCGAGGTCTATGCATAAAAAGCAGCGGCTGACGTCTTCCGACGTTGGCCGTTTT‑T‑T‑TATGTGTAAGGAACTTCTATGAATACTGTCGGCACGCCGTTGCTATGGGGCGGATTCGCTGTTGTTGTCGCCAtt                                                         >  2:7661171/1‑140 (MQ=255)

                                                                                 tGCCGGAAGGCGAGGTCTATGCATAAAAAGCAGCGGCTGACGTCTTCCGACGTTGGCCGTTTT‑T‑T‑TATGTGTAAGGAACTTCTATGAATACTGTCGGCACGCCGTTGCTATGGGGCGGATTCGCTGTTGTTGTCGCCAtt                                                         >  2:4483150/1‑140 (MQ=255)

                                                                                 tGCCGGAAGGCGAGGTCTATGCATAAAAAGCAGCGGCTGACGTCTTCCGACGTTGGCCGTTTT‑T‑T‑TATGTGTAAGGAACTTCTAAGAATACTGTCGGCACGCCGTTGCTATGGGGCGGATTCGCTGTTGTTGTCGCCAtt                                                         >  2:2222565/1‑140 (MQ=255)

                                                                                 tGCCGGAAGGCGAGGTCTATGCATAAAAAGCAGCGGCTGACGTCTGCCGACGTTGGCCGTTTT‑T‑T‑TTTGTGTAAGGAACTTCTATTAATACTGTCGGCACGCCGTTGCTATTGGGCGGATTCGCTGTTGTTGTCGCCAtt                                                         <  2:9326908/140‑1 (MQ=255)

                                                                                 tGCCGGAAGGCGAGATCTATGCATAAAAAGCAGCGGCTGACGTCTTCCGACGTTGGCCGTTTT‑T‑T‑TATGTGTAAGGAACTTCTATGAATACTGTCGGCACGCCGTTGCTATGGGGCGGATTCGCTGTTGTTGTCGCCAtt                                                         >  1:2741343/1‑140 (MQ=255)

                                                                                  gCCGGAAGGCGAGGTCTATGCATAAAAAGCAGCGGCTGACGTCTTCCGACGTTGGCCGTTTT‑T‑T‑TATGTGTAAGGAACTTCTATGAATACTGTCGGCACGCCGTTGCTATGGGGCGGATTCGCTGTTGTTGTCGCCATTa                                                        >  1:7406367/1‑140 (MQ=255)

                                                                                   ccGGAAGGCGAGGTCTATGCATAAAAAGCAGCGGCTGACGTCTTCCGACGTTGGCCGTTTT‑T‑T‑TATGTGTAAGGAACTTCTATGAATACTGTCGGCACGCCGTTGCTATGGGGCGGATTCGCTGTTGTTGTCGCCATTAt                                                       >  2:4215516/1‑140 (MQ=255)

                                                                                     ggAAGGCGAGGTCTATGCATAAAAAGCAGCGGCTGACGTCTTCCGACGTTGGCCGTTTT‑T‑T‑TATGTGTAAGGAACTTCTATGAATACTGTCGGCACGCCGTTGCTATGGGGCGGATTCGCTGTTGTTGTCGCCATTATGc                                                     <  1:8859148/140‑1 (MQ=255)

                                                                                      gAAGGCGAGGTCTATGCATAAAAAGCAGCGGCTGACGTCTTCCGACGTTGGCCGTTTT‑T‑T‑TATGTGTAAGGAACTTCTATGAATACTGTCGGCACGCCGTTGCTATGGGGCGGATTCGCTGTTGTTGTCGCCATTATGCt                                                    >  1:9291817/1‑140 (MQ=255)

                                                                                      gAAGGCGAGGTCTATGCATAAAAAGCAGCGGCTGACGTCTTCCGACGTTGGCCGTTTT‑T‑T‑TATGTGTAAGGAACTTCTATGAATACTGTCGGCACGCCGTTGCTATGGGGCGGATTCGCTGTTGTTGTCGCCATTATGCt                                                    <  2:8211011/140‑1 (MQ=255)

                                                                                      gAAGGCGAGGTCTATGCATAAAAAGCAGCGGCTGACGTCTTCCGACGTTGGCCGTTTT‑T‑T‑TATGTGTAAGGAACTTCTATGAATACTGTCGGCACGCCGTTGCTATGGGGCGGATTCGCTGTTGTTGTCGCCATTATGCt                                                    <  2:6934823/140‑1 (MQ=255)

                                                                                                tCTATGCATAAAAAGCAGCGGCTGACGTCTTCCGACGTTGGCCGTTTT‑T‑T‑TATGTGTAAGGAACTTCTATGAATACTGTCGGCACGCCGTTGCTATGGGGCGGATTCGCTGTTGTTGTCGCCATTATGCTGGCTATCGAc                                          <  1:5830246/140‑1 (MQ=255)

                                                                                                    tGCATAAAACGCAGCGGCTGAGGTCTTCCGACGTTGGCCGTTTT‑T‑T‑TATGTGTAAGGAACTTCTCTGAATACTGTGGGGACGCCGGTGCTATGGGGCGGATTCGCTGTTGTTGTCGCCATTATGCTGGCTATCGACCtgt                                      <  2:7255933/140‑1 (MQ=255)

                                                                                                    tGCATAAAAAGCAGCGGCTGACGTCTTCCGACGTTGGCCGTTTT‑T‑T‑TATGTGTAAGGAACTTCTATGAATACTGTCGGCACGCCGTTGCTATGGGGCGGATTCGCTGTTGTTGTCGCCATTATGCTGGCTATCGACCtgt                                      >  1:3254543/1‑140 (MQ=255)

                                                                                                    tGCATAAAAAGCAGCGGCTGACGTCTTCCGACGTTGGCCGTTTT‑T‑T‑TATGTGTAAGGAACTTCTATGAATACTGTCGGCACGCCGTTGCTATGGGGCGGATTCGCTGTTGTTGTCGCCATTATGCTGGCTATCGACCtgt                                      >  2:8551940/1‑140 (MQ=255)

                                                                                                         aaaaaGCAGCGGCTGACGTCTTCCGACGTTGGCCGTTTT‑T‑T‑TATGTGTAAGGAACTTCTATGAATACTGTCGGCACGCCGTTGCTATGGGGCGGATTCGCTGTTGTTGTCGCCATTATGCTGGCTATCGACCTGttgttg                                 >  1:6422607/1‑140 (MQ=255)

                                                                                                         aaaaaGCAGCGGCTGACGTCTTCCGACGTTGGCCGTTTT‑T‑T‑TATGTGTAAGGAACTTCTATGAATACTGTCGGCACGCCGTTGCTATGGGGCGGATTCGCTGTTGTTGTCGCCATTATGCTGGCTATCGACCTGttgttg                                 >  1:5145635/1‑140 (MQ=255)

                                                                                                         aaaaaGCAGCGGCTGACGTCTTCCGACGATGGCCG‑TTT‑TGT‑TATGTGTAAGGAACTTCTATGAATACTGTCGGCACGCCGGTGCTATGGGGCGGATTCGCTGTGGTTGTCGCCAGTCTGCTGGCTATCGACCTGttgttg                                 >  2:2113439/1‑140 (MQ=255)

                                                                                                          aaaaGCAGCGGCTGACGTCTTCCGACGTTGGCCGTTTT‑T‑T‑TATGTGTAAGGAACTTCTATGAATACTGTCGGCACGCCGTTGCTATGGGGCGGATTCGCTGTTGTTGTCGCCATTATGCTGGCTATCGACCTGTTGTTGc                                >  2:6115897/1‑140 (MQ=255)

                                                                                                          aaaaGCAGCGGCTGACGTCTTCCGACGTTGGCCGTTTT‑T‑T‑TATGTGTAAGGAACTTCTATGAATACTGTCGGCACGCCGTTGCTATGGGGCGGATTCGCTGTTGTTGTCGCCATTATGCTGGCTATCGACCGGTTGTTGc                                >  1:3361964/1‑140 (MQ=255)

                                                                                                              gcagcGGCTGACGTCTTCCGACGTTGGCCGTTTT‑T‑T‑TATGTGTAAGGAACTTCTATGAATACTGTCGGCACGCCGTTGCTATGGGGCGGATTCGCTGTTGTTGTCGCCATTATGCTGGCTATCGACCTGTTGTTGCAggg                            >  2:8907831/1‑140 (MQ=255)

                                                                                                              gcagcGGCTGACGTCTTCCGACGTTGGCCGTTTT‑T‑T‑TATGTGTAAGGAACTTCTATGAATACTGTCGGCACGCCGTTGCTATGGGGCGGATTCGCTGTTGTTGTCGCCATTATGCTGGCTATCGACCTGTTGTTGCAggg                            >  1:7289688/1‑140 (MQ=255)

                                                                                                                agcGGCTGACGTCTTCCGACGTTGGCCGTTTT‑T‑T‑TATGTGTAAGGAACTTCTATGAATACTGTCGGCACGCCGTTGCTATGGGGCGGATTCGCTGTTGTTGTCGCCATTATGCTGGCTATCGACCTGTTGTTGCAGGGGc                          >  2:3053088/1‑140 (MQ=255)

                                                                                                                 gcGGCTGACGTCTTCCGACGTTGGCCGTTTT‑T‑T‑TATGTGTAAGGACCTTCTATGAATACTGTCGGCACGCCGGTGCTATGGGGCGGATTCGCTGTTGTTGTCGCCATTATGCTGGCTATCGACCTGTTGTTGCAGGGGcg                         >  2:395671/1‑140 (MQ=255)

                                                                                                                 gcGGCTGACGTCTTCCGACGTTGGCCGTTTT‑T‑T‑TATGTGTAAGGAACTTCTATGAATACTGTCGGCACGCCGTTGCTATGGGGCGGATTCGCTGTTGTTGTCGCCATTATGCTGGCTATCGACCTGTTGTTGCAGGGGcg                         <  1:6859010/140‑1 (MQ=255)

                                                                                                                 gcGGCTGACGTCTTCCGACGTTGGCCGTTTT‑T‑T‑TATGTGTAAGGAACTTCTATGAATACTGTCGGCACGCCGTTGCTATGGGGCGGATTCGCTGTTGTTGTCGCCATTATGCTGGCTATCGACCTGTTGTTGCAGGGGcg                         >  1:2915027/1‑140 (MQ=255)

                                                                                                                 gcGGCTGACGTCTTCCGACGTTGGCCGTTTT‑T‑T‑TATGTGTAAGGAACTTCTATGAATACTGTCGGCACGCCGTTGCTATGGGGCGGATTCGCTGTTGTTGTCGCCATTATGCTGGCTATCGACCTGTTGTTGCAGGGGcg                         >  2:403537/1‑140 (MQ=255)

                                                                                                                 gcGGCTGACGTCTTCCGACGTTGGCCGTTTT‑T‑T‑TATGTGTAAGGAACTTCTATGAATACTGTCGGCACGCCGTTGCTATGGGGCGGATTCGCTGTTGTTGTCGCCATTATGCTGGCTATCGACCTGTTGTTGCAGGGGcg                         >  1:9142389/1‑140 (MQ=255)

                                                                                                                    gCTGACGTCTTCCGACGTTGGCCGTTTT‑T‑T‑TATGTGTAAGGAACTTCTATGAATACTGTCGGCACGCCGTTGCTATGGGGCGGATTCGCTGTTGTTGTCGCCATTATGCTGGCTATCGACCTGTTGTTGCAGGGGcgtcg                      <  1:6749531/140‑1 (MQ=255)

                                                                                                                     cTGACGTCTTCCGACGTTGGCCGTTTT‑T‑T‑TATGTGTAAGGAACTTCTATGAATACTGTCGGCACGCCGTTGCTATGGGGCGGATTCGCTGTTGTTGTCGCCATTATGCTGGCTATCGACCTGTTGTTGCAGGGGcgtcgt                     <  1:1864804/140‑1 (MQ=255)

                                                                                                                     cTGACGTCTTCCGACGTTGGCCG‑TTT‑TAT‑TATGTGTAAGGAACTTCTATGAATACTGTCGGCACGCCGTTGCTATGGGGCGGATTCGCTGTTGTTGTCGCCATTATGCTGGCTATCGACCTGTTGTTGCAGGGGcgtcgt                     <  2:5348514/140‑1 (MQ=255)

                                                                                                                      tGACGTCTTCCGACGTTGGCCGTTTT‑T‑T‑TATGTGTAAGGAACTTCTATGAATACTGTCGGCACGCCGTTGCTATGGGGCGGATTCGCTGTTGTTGTCGCCATTATGCTGGCTATCGACCTGTTGTTGCAGGGGCGTCGTg                    <  1:9041661/140‑1 (MQ=255)

                                                                                                                            cTTCCGACGTTGGCCGTTTT‑T‑T‑TATGTGTAAGGAACTTCTATGAATACTGTCGGCACGCCGTTGCTATGGGGCGGATTCGCTGTTGTTGTCGCCATTATGCTGGCTATCGACCTGTTGTTGCAGGGGCGTCGTGGGGcac              >  1:477046/1‑140 (MQ=255)

                                                                                                                            cTTCCGACGTTGGCCGTTTT‑T‑T‑TATGTGTAAGGAACTTCTATGAATACTGTCGGCACGCCGTTGCTATGGGGCGGATTCGCTGTTGTTGTCGCCATTATGCTGGCTATCGACCTGTTGTTGCAGGGGCGTCGTGGGGcac              <  1:3932926/140‑1 (MQ=255)

                                                                                                                             ttCCGACGTTGGCCGTTTT‑T‑T‑TATGTGTAAGGAACTTCTATGAATACTGTCGGCACGCCGTTGCTATGGGGCGGATTCGCTGTTGTTGTCGCCATTATGCTGGCTATCGACCTGTTGTTGCAGGGGCGTCGTGGGGcaca             <  2:1949376/140‑1 (MQ=255)

                                                                                                                               ccGACGTTGGCCGTTTT‑T‑T‑TATGTGTAAGGAACTTCTATGAATACTGTCGGCACGCCGTTGCTATGGGGCGGATTCGCTGTTGTTGTCGCCATTATGCTGGCTATCGACCTGTTGTTGCAGGGGCGTCGTGGGGCACATg           >  1:812904/1‑140 (MQ=255)

                                                                                                                               ccGACGTTGGCCGTTTT‑T‑T‑TATGTGTAAGGAACTTCTATGAATACTGTCGGCACGCCGTTGCTATGGGGCGGATTCGCTGTTGTTGTCGCCATTATGCTGGCTATCGACCTGTTGTTGCAGGGGCGTCGTGGGGCACATg           >  1:9393771/1‑140 (MQ=255)

                                                                                                                                   cGTTGGCCGTTTT‑T‑T‑TATGTGTAAGGACCTTCTATGAATACTGTCGGCACGCCGTTGCTATGGGGCGGATTCGCTGTTGTTGTCGCCATTATGCTGGCTATCGACCTGTTGTTGCAGGGGCGTCGTGGGGCACATGccat       <  2:7748896/140‑1 (MQ=255)

                                                                                                                                   cGTTGGCCGTTTT‑T‑T‑TATGTGTAAGGAACTTCTATGAATACTGTCGGCACGCCGTTGCTATGGGGCGGATTCGCTGTTGTTGTCGCCATTATGCTGGCTATCGACCTGTTGTTGCAGGGGCGTCGTGGGGCACATGccat       <  2:8023838/140‑1 (MQ=255)

                                                                                                                                   cGTTGGCCGTTTT‑T‑T‑TATGTGTAAGGAACTTCTATGAATACTGTCGGCACGCCGTTGCTATGGGGCGGATTCGCTGTTGTTGTCGCCATTATGCTGGCTATCGACCTGTTGTTGCAGGGGCGTCGTGGGGCACATGccat       <  1:3565040/140‑1 (MQ=255)

                                                                                                                                    gTTGGCCGTTTT‑T‑T‑TATGTGTAAGGAACTTCTATGAATACTGTCGGCACGCCGTTGCTATGGGGCGGATTCGCTGTTGTTGTCGCCATTATGCTGGCTATCGACCTGTTGTTGCAGGGGCGTCGTGGGGCACATGccatg      >  1:661926/1‑140 (MQ=255)

                                                                                                                                     ttGGCCGTTTT‑T‑T‑TATGTGTAAGGAACTTCTATGAATACTGTCGGCACGCCGTTGCTATGGGGCGGATTCGCTGTTGTTGTCGCCATTATGCTGGCTATCGACCTGTTGTTGCAGGGGCGTCGTGGGGCACATGccatga     <  2:5428106/140‑1 (MQ=255)

                                                                                                                                       ggCCGTTTT‑T‑T‑TATGTGTAAGGAACTNCTATGAATACTGTCGGCACGCCGTTGCTATGGGGCGGATTCGCTGTTGTTGTCGCCATTATGCTGGCTATCGACCTGTTGTTGCAGGGGCGTCGTGGGGNACATGccatgacc   <  1:6227817/140‑1 (MQ=255)

                                                                                                                                        gCCG‑TTT‑T‑TATATGTGTAATGAACTTCTAAGAATACTGTCGGCACGCCGTTGCTATGGGGCGGATTCGCTGTTGTTGTCGCCATTATGCTGGCTATCGACCTGTTGTTGCAGGGGCGTCGTGGGGCACATGccatgacca  >  2:2814785/1‑140 (MQ=255)

TGTGTGAAAAAACACATCCGGTCACCGGGCAACCCGAAAGGAATACGCAGACGTATTCCTTTTTTGTTGTAAGTGAGACCTTGCCGGAAGGCGAGGTCTATGCATAAAAAGCAGCGGCTGACGTCTTCCGACGTTGGCCG‑TTT‑T‑T‑TATGTGTAAGGAACTTCTATGAATACTGTCGGCACGCCGTTGCTATGGGGCGGATTCGCTGTTGTTGTCGCCATTATGCTGGCTATCGACCTGTTGTTGCAGGGGCGTCGTGGGGCACATGCCATGACCA  >  NZ_CP025268/3239102‑3239376

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`