BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP025268	3,982,879	(G)_8→9	pseudogene (73/1388 nt)	yifK →	amino acid permease

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP025268	3,982,879	1	.	G	95.4%	1727.0 / 70.8	520	pseudogene (73/1388 nt)	yifK	amino acid permease
Reads supporting (aligned to +/- strand): ref base . (17/7); new base G (218/276); total (236/284)
Fisher's exact test for biased strand distribution p-value = 1.17e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 531 total aligned reads displayed.

GATGTACCCATAACGATAACCGGTAACACCGGAAAGCATGCAAACACAACACGAGGATTTATGGCAGATAACAAACCAGAGCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCCTGGGGGGGG‑CACCATTGGCGTCGGCCTGTTTATGGGGGCCGCCAGTACCCTGAAATGGGCCGGGCCATCCGTATTGTTGGCCTATATCATCGCCGGGCTGTTCGTCTTTTTCATCATGCGTTCAATGGGCGAAATGTTGTTCCTCGAA  >  NZ_CP025268/3982747‑3983018

ctcgatgtacccataacgataaccggtaacaccggaaagcatgcaaacacaacacgaggatttatggcagataacaaaccagagctacagcgtgggctggaagctcgacatatcgaactcatcgccctGGGGGGCACCAt                                                                                                                                       >  1:6183852‑M1/129‑140 (MQ=255)

      gtacccataacgataaccggtaacaccggaaagcatgcaaacacaacacgaggatttatggcagataacaaaccagagctacagcgtgggctggaagctcgacatatcgaactcatcgccctGGGGGGCACCATTGGCGt                                                                                                                                 >  2:6099076‑M1/123‑140 (MQ=255)

         cccataacgataaccggtaacaccggaaagcatgcaaacacaacacgaggatttatggcagataacaaaccagagctacagcgtgggctggaagctcgacatatcgaactcatcgccctGGGGGGCACCATTGGCGTCgg                                                                                                                              <  1:8683058‑M1/21‑1 (MQ=255)

         cccataacgataaccggtaacaccggaaagcatgcaaacacaacacgaggatttatggcagataacaaaccagagctacagcgtgggctggaagctcgacatatcgaactcatcgccctGGGGGGCACCATTGGCGTCgg                                                                                                                              <  1:2063181‑M1/21‑1 (MQ=255)

          ccataacgataaccggtaacaccggaaagcatgcaaacacaacccgaggatttatggcagataacagaccagagctacagcgtgggctggaagctcgacatatcgaactcatcgccctGGGGGGCACCATTGGCGTCGGc                                                                                                                             >  1:8356001‑M1/119‑140 (MQ=255)

          ccataacgataaccggtaacaccggaaagcatgcaaacacaacacgaggatttatggcagataacaaaccagagctacagcgtgggctggaagctcgacatatcgaactcatcgccctGGGGGGCACCATTGGCGTCGGc                                                                                                                             >  1:9452631‑M1/119‑140 (MQ=255)

          ccataacgataaccggtaacaccggaaagcatgcaaacacaacacgaggatttatggcagataacaaaccagagctacagcgtgggctggaagctcgacatatcgaactcatcgccctGGGGGGCACCATTGGCGTCGGc                                                                                                                             >  1:1943236‑M1/119‑140 (MQ=255)

          ccataacgataaccggtaacaccggaaagcatgcaaacacaacacgaggatttatggcagataacaaaccagagctacagcgtgggctggaagctcgacatatcgaactcatcgccctGGGGGGCACCATTGGCGTCGGc                                                                                                                             >  2:6701999‑M1/119‑140 (MQ=255)

           cataacgataaccggtaacaccggaaagcatgcaaacacaacacgaggatttatggcagataacaaaccagagctacagcgtgggctggaagctcgacatatcgaactcatcgccctGGGGGGCACCATTGGCGTCGGcc                                                                                                                            >  1:7761786‑M1/118‑140 (MQ=255)

             taacgataaccggtaacaccggaaagcatgcaaacacaacacgaggatttatggcagataacaaaccagagctacagcgtgggctggaagctcgacatatcgaactcatcgccctGGGGGGCACCATTGGCGTCGGCCTg                                                                                                                          <  1:1193794‑M1/25‑1 (MQ=255)

             taacgataaccggtaacaccggaaagcatgcaaacacaacacgaggatttatggcagataacaaaccagagctacagcgtgggctggaagctcgacatatcgaactcatcgccctGGGGGGCACCATTGGCGTCGGCCTg                                                                                                                          <  1:1878348‑M1/25‑1 (MQ=255)

             taacgataaccggtaacaccggaaagcatgcaaacacaacacgaggatttatggcagataacaaaccagagctacagcgtgggctggaagctcgacatatcgaactcatcgccctGGGGGGCACCATTGGCGTCGGCCTg                                                                                                                          <  1:5536286‑M1/25‑1 (MQ=255)

              aacgataaccggtaacaccggaaagcatgcaaacacaacacgaggatttatggcagataacaaaccagagctacagcgtgggctggaagctcgacatatcgaactcatcgccctGGGGGGCACCATTGGCGTCGGCCTGt                                                                                                                         <  2:3444759‑M1/26‑1 (MQ=255)

              aacgataaccggtaacaccggaaagcatgcaaacacaacacgaggatttatggcagataacaaaccagagctacagcgtgggctggaagctcgacatatcgaactcatcgccctGGGGGGCACCATTGGCGTCGGCCTGt                                                                                                                         <  1:3479872‑M1/26‑1 (MQ=255)

               acgataaccggtaacaccggaaagcatgcaaacacaacacgaggatttatggcagataacaaaccagagctacagcgtgggctggaagctcgacatatcgaactcatcgccctGGGGGGCACCATTGGCGTCGGCCTGtt                                                                                                                        <  1:1362759‑M1/27‑1 (MQ=255)

               acgataaccggtaacaccggaaagcatgcaaacacaacacgaggatttatggcagataacaaaccagagctacagcgtgggctggaagctcgacatatcgaactcatcgccctGGGGGGCACCATTGGCGTCGGCCTGtt                                                                                                                        <  1:2258844‑M1/27‑1 (MQ=255)

               acgataaccggtaacaccggaaagcatgcaaacacaacacgaggatttatggcagataacaaaccagagctacagcgtgggctggaagctcgacatatcgaactcatcgccctGGGGGGCACCAGTGGCGtcggccgggt                                                                                                                        <  2:7441828‑M1/27‑5 (MQ=255)

               acgataaccggtaacaccggaaagcatgcaaacacaacacgaggatttatggcagaaaacaaaccagagctacagcgtgggctggaagctcgacatatcgaactcatcgccctGGGGGGCACCATTGGCGTCGGCCTGtt                                                                                                                        <  2:7065223‑M1/27‑1 (MQ=255)

                 gataaccggtaacaccggaaagcatgcaaacacaacacgaggatttatggcagataacaaaccagagctacagcgtgggctggaagctcgacatatcgaactcatcgccctGGGGGGCACCATTGGCGTCGGCCTGTTTa                                                                                                                      <  2:3087942‑M1/29‑1 (MQ=255)

                 gataaccggtaacaccggaaagcatgcaaacacaacacgaggatttatggcagataacaaaccagagctacagcgtgggctggaagctcgacatatcgaactcatcgccctGGGGGGCACCATTGGCGTCGGCCTGTTTa                                                                                                                      <  1:2152236‑M1/29‑1 (MQ=255)

                   taaccggtaacaccggaaagcatgcaaacacaacacgaggatttatggcagataacaaaccagagctacagcgtgggctggaagctcgacatatcgaactcatcgccctGGGGGGCACCATTGGCGTCGGCCTGTTTATg                                                                                                                    <  1:2446182‑M1/31‑1 (MQ=255)

                   taaccggtaacaccggaaagcatgcaaacacaacacgaggatttatggcagataacaaaccagagctacagcgtgggctggaagctcgacatatcgaactcatcgccctGGGGGGCACCATTGGCGTCGGCCTGTTTATg                                                                                                                    <  1:4947133‑M1/31‑1 (MQ=255)

                    aaccggtaacaccggaaagcatgcaaacacaacacgaggatttatggcagataacaaaccagagctacagcgtgggctggaagctcgacatatcgaactcatcgccctGGGGGGCACCATTGGCGTCGGCCTGTTTATgg                                                                                                                   <  1:5478416‑M1/32‑1 (MQ=255)

                      ccggtaacaccggaaagcatgcaaacacaacacgaggatttatggcagataacaaaccagagctacagcgtgggctggaagctcgacatatcgaactcatcgccctGGGGGGCACCATTGGCGTCGGCCTGTTTATgggg                                                                                                                 <  2:6464988‑M1/34‑1 (MQ=255)

                      ccggtaacaccggaaagcatgcaaacacaacacgaggatttatggcagataacaaaccagagctacagcgtgggctggaagctcgacatatcgaactcatcgccctGGGGGGCACCATTGGCGTCGGCCTGTTTATgggg                                                                                                                 >  2:5978219‑M1/107‑140 (MQ=255)

                      ccggtaacaccggaaagcatgcaaacacaacacgaggatttatggcagataacaaaccagagctacagcgtgggctggaagctcgacatatcgaactcatcgccctGGGGGGCACCATTGGCGTCGGCCTGTTTATgggg                                                                                                                 <  1:2347069‑M1/34‑1 (MQ=255)

                      ccggtaacaccggaaagcatgcaaacacaacacgaggatttatggcagataacaaaccagagctacagcgtgggctggaagctcgacatatcgaactcatcgccctGGGGGGCACCATTGGCGTCGGCCTGTTTATgggg                                                                                                                 >  1:3877217‑M1/107‑140 (MQ=255)

                       tAACACCGGAATGCATGCAAACACACCACGAGGCTTTCTGGCAGATAACAAACCAGAGCTACAGCGTGGGCTGGAAGCTCGACATATCGAGCTCATCGGC‑‑GTGGGGGG‑CACCTTGGGCGTCGGCGGGGGTATGGGGgccg                                                                                                             >  2:1922727/1‑140 (MQ=255)

                        ggtaacaccggaaagcatgcaaacacaacacgaggatttatggcagataacaaaccagagctacagcgtgggctggaagctcgacatatcgaactcatcgccctGGGGGGCACCATTGGCGTCGGCCTGTTTATGGGGgc                                                                                                               >  1:8156111‑M1/105‑140 (MQ=255)

                        ggtaacaccggaaagcatgcaaacacaacacgaggatttatggcagataacaaaccagagctacagcgtgggctggaagctcgacatatcgaactcatcgccctGGGGGGCACCATTGGCGTCGGCCTGTTTATGGGGgc                                                                                                               <  1:2187117‑M1/36‑1 (MQ=255)

                        ggtaacaccggaaagcatgcaaacacaacacgaggatttatggcagataacaaaccagagctacagcgtgggctggaagctcgacatatcgaactcatcgccctGGGGGGCACCATTGGCGTCGGCCTGTTTATGGGGgc                                                                                                               >  1:4488247‑M1/105‑140 (MQ=255)

                        ggtaacaccggaaagcatgcaaacacaacacgaggatttatggcagataacaaaccagagctacagcgtgggctggaagctcgacatatcgaactcatcgccctGGGGGGCACCATTGGCGTCGGCCTGTTTATGGGGgc                                                                                                               >  2:8881437‑M1/105‑140 (MQ=255)

                        gggaacaccggacagcatgcaaacacaacacgaggatttatggcagataacaaacaagagctacagcgtgggctggacgctcgacatatcgaactcatcgccctGGGGGGCACCATTGGCGTCGGCCTGGTTAGGGGGgc                                                                                                               <  2:3672400‑M1/36‑1 (MQ=255)

                          taacaccggaaagcatgcaaacacaacacgaggatttatggcagataacaaaccagagctacagcgtgggctggaagctcgacatatcgaactcatcgccctGGGGGGCACCATTGGCGTCGGCCTGTTTATGGGGgccg                                                                                                             <  1:2830632‑M1/38‑1 (MQ=255)

                            acaccggaaagcatgcaaacacaacacgaggatttatggcagataacaaaccagagctacagcgtgggctggaagctcgacatatcgaactcatcgccctGGGGGGCACCATTGGCGTCGGCCTGTTTATGGGGgccgcc                                                                                                           <  1:3949381‑M1/40‑1 (MQ=255)

                             caccggaaagcatgcaaacacaacacgaggatttatggcagataacaaaccagagctacagcgtgggctggaagctcgacatatcgaactcatcgccctGGGGGGCACCATTGGCGTCGGCCTGTTTATGGGGGCCGCCa                                                                                                          >  2:871402‑M1/100‑140 (MQ=255)

                             caccggaaagcatgcaaacacaacacgaggatttatggcagataacaaaccagagctacagcgtgggctggaagctcgacatatcgaactcatcgccctGGGGGGCACCATTGGCGTCGGCCTGTTTATGGGGGCCGCCa                                                                                                          >  1:1765534‑M1/100‑140 (MQ=255)

                                cggaaagcatgcaaacacaacacgaggatttatggcagataacaaaccagagctacagcgtgggctggaagctcgacatatcgaactcatcgccctGGGGGGCACCATTGGCGTCGGCCTGTTTATGGGGGCCGCCAGTa                                                                                                       <  2:5393019‑M1/44‑1 (MQ=255)

                                 ggaaagcatgcaaacacaacacgaggatttatggcagataacaaaccagagctacagcgtgggctggaagctcgacatatcgaactcatcgccctGGGGGGCACCATTGGCGTCGGCCTGTTTATGGGGGCCGCCAGTAc                                                                                                      <  1:3642557‑M1/45‑1 (MQ=255)

                                 ggaaagcatgcaaacacaacacgaggatttatggcagataacaaaccagagctacagcgtgggctggaagctcgacatatcgaactcatcgccctGGGGGGCACCATTGGCGTCGGCCTGTTTATGGGGGCCGCCAGTAc                                                                                                      <  2:2983958‑M1/45‑1 (MQ=255)

                                    aagcatgcaaacacaacacgaggatttatggcagataacaaaccagagctacagcgtgggctggaagctcgacatatcgaactcatcgccctGGGGGGCACCCTTGGCGTCGGCCTGTTTATGGGGGCCGCCAGTACCCt                                                                                                   <  2:5306314‑M1/48‑1 (MQ=255)

                                       catgcaaacacaacacgaggatttatggcagataacaaaccagagctacagcgtgggctggaagctcgacatatcgaactcatcgccctGGGGGGCACCATTGGCGTCGGCCTGTTTATGGGGGCCGCCAGTACCCTGaa                                                                                                >  2:1691202‑M1/90‑140 (MQ=255)

                                       catgcaaacacaacacgaggatttatggcagataacaaaccagagctacagcgtgggctggaagctcgacatatcgaactcatcgccctGGGGGGCACCATTGGCGTCGGCCTGTTTATGGGGGCCGCCAGTACCCTGaa                                                                                                <  1:152675‑M1/51‑1 (MQ=255)

                                          gcaaacacaacacgaggatttatggcagataacaaaccagagctacagcgtgggctggaagctcgacatatcgaactcatcgccctGGGGGGCACCATTGGCGTCGGCCTGTTTATGGGGGCCGCCAGTACCCTGAAATg                                                                                             <  2:9067241‑M1/54‑1 (MQ=255)

                                                acaacacgaggatttatggcagataacaaaccagagctacagcgtgggctggaagctcgacatatcgaactcatcgccctGGGGGGCACCATTGGCGTCGGCCTGTTTATGGGGGCCGCCAGTACCCTGAAATgggccgg                                                                                       >  1:2600485‑M1/81‑140 (MQ=255)

                                                  cacacgaggatttatggcagataacaaaccagagctacagcgtgggctggaagctcgacatatcgaactcatcgccctGGGGGGCACCATTGGCGTCGGCCTGTTTATGGGGGCCGCCAGTACCCTGAAATgggccgggc                                                                                     >  1:469298‑M1/79‑140 (MQ=255)

                                                  aacacgaggatttatggcagataacaaaccagcgctacagcgtgggctggaagctcgacatctcgaactcatcgccctGGGGGGCACCATTGGCGTCGGCCTGTTTATGGGGGCCGCCAGTACCCTGAAATgggccgggc                                                                                     >  1:5760251‑M1/79‑140 (MQ=255)

                                                  aacacgaggatttatggcagataacaaaccagagctacagcgtgggctggaagctcgacatatcgaactcatcgccctGGGGGGCACCATTGGCGTCGGCCTGTTTATGGGGGCCGCCAGTACCCTGAAATgggccgggc                                                                                     >  1:7905443‑M1/79‑140 (MQ=255)

                                                  aacacgaggatttagggcagataacaaaccagagctacagcgtgggctggaagctcgacatatcgaactcatcgccctGGGGGGCACCATTGGCGTCGGCCTGTTTATGGGGGCCGCCAGTACCCTGAAATgggccgggc                                                                                     >  1:693081‑M1/79‑140 (MQ=255)

                                                   acacgaggatttatggcagataacaaaccagagctacagcgtgggctggaagctcgacatatcgaactcatcgccctGGGGGGCACCATTGGCGTCGGCCTGTTTATGGGGGCCGCCAGTACCCTGAAATgggccgggcc                                                                                    >  1:2974547‑M1/78‑140 (MQ=255)

                                                    cacgaggatttatggcagataacaaaccagagctacagcgtgggctggaagctcgacatatcgaactcatcgccctGGGGGGCACCATTGGCGTCGGCCTGTTTATGGGGGCCGCCAGTACCCTGAAATGGGCCGGGCCa                                                                                   <  1:850444‑M1/64‑1 (MQ=255)

                                                     acgaggatttatggcagataacaaaccagagctacagcgtgggctggaagctcgacatatcgaactcatcgccctGGGGGGCACCATTGGCGTCGGCCTGTTTATGGGGGCCGCCAGTACCCTGAAATGGGCCGGGCCAt                                                                                  >  1:3280003‑M1/76‑140 (MQ=255)

                                                      cgaggatttatggcagataacaaaccagagctacagcgtgggctggaagctcgacatatcgaactcatcgccctGGGGGGCACCATTGGCGTCGGCCTGTTTATGGGGGCCGCCAGTACCCTGAAATGGGCCGGGCCATc                                                                                 <  2:440438‑M1/66‑1 (MQ=255)

                                                       tagtatttatggcatataacaaaccagagctacagcgtgggctggaagctcgacatatcgaactcatcgccctGGGGG‑CACCATTGGCGTCGGCCTGTTTATGGGGGCCGCCAGTACCCTGAAATGGGCCGGGCCATCCg                                                                               >  2:4829984‑M1/74‑140 (MQ=255)

                                                       gaggatttatggcagataacaaaccagagctacagcgtgggctggaagctcgacatatcgaactcatcgccctGGGGGGCACCATTGGCGTCGGCCTGTTTATGGGGGCCGCCAGTACCCTGAAATGGGCCGGGCCATcc                                                                                >  1:7386560‑M1/74‑140 (MQ=255)

                                                        aggatttatggcagataacaaaccagagctacagcgtgggctggaagctcgacatatcgaactcatcgccctGGGGGGCACCATTGGCGTCGGCCTGTTTATGGGGGCCGCCAGTACCCTGAAATGGGCCGGGCCATCCg                                                                               >  1:2641976‑M1/73‑140 (MQ=255)

                                                            tttatggcagataacaaaccagagctacagcgtgggctggaagctcgacatatcgaactcatcgccctGGGGGGCACCATTGGCGTCGGCCTGTTTATGGGGGCCGCCAGTACCCTGAAATGGGCCGGGCCATCCGTAtt                                                                           <  2:5595357‑M1/72‑1 (MQ=255)

                                                            tttatggcagataacaaaccagagctacagcgtgggctggaagctcgacatatcgaactcatcgccctGGGGGGCACCATTGGCGTCGGCCTGTTTATGGGGGCCGCCAGTACCCTGAAATGGGCCGGGCCATCCGTAtt                                                                           <  2:8230514‑M1/72‑1 (MQ=255)

                                                             ttatggcagataacaaaccagagctacagcgtgggctggaagctcgacatatcgaactcatcgccctGGGGGGCACCATTGGCGTCGGCCTGTTTATGGGGGCCGCCAGTACCCTGAAATGGGCCGGGCCATCCGTAttg                                                                          >  2:2705064‑M1/68‑140 (MQ=255)

                                                             ttatggcagataacaaaccagagctacagcgtgggctggaagctcgacatatcgaactcatcgccctGGGGGGCACCATTGGCGTCGGCCTGTGTATGGGGGCCGCCAGTACCCTGAAATGGGCCGGGCCATCCGTAttg                                                                          >  2:3809446‑M1/68‑140 (MQ=255)

                                                              tatggcagataacaaaccagagctacagcgtgggctggaagctcgacatatcgaactcatcgccctGGGGGGCACCATTGGCGTCGGCCTGTTTATGGGGGCCGCCAGTACCCTGAAATGGGCCGGGCCATCCGTAttgt                                                                         >  1:1586439‑M1/67‑140 (MQ=255)

                                                              tatggcagataacaaaccagagctacagcgtgggctggaagctcgacatatcgaactcatcgccctGGGGGGCACCATTGGCGTCGGCCTGTTTATGGGGGCCGCCAGTACCCTGAAATGGGCCGGGCCATCCGTAttgt                                                                         >  2:2142887‑M1/67‑140 (MQ=255)

                                                              tatggcagataacaaaccagagctacagcgtgggctggaagctcgacatatcgaactcatcgccctGGGGGGCACCATTGGCGTCGGCCTGTTTATGGGGGCCGCCAGTACCCTGAAATGGGCCGGGCCATCCGTAttgt                                                                         >  2:1125605‑M1/67‑140 (MQ=255)

                                                              tatggcagataacaaaccagagctacagcgtgggctggaagctcgacatatcgaactcatcgccctGGGGGGCACCATTGGCGTCGGCCTGTTTATGGGGGCCGCCAGTACCCTGAAATGGGCCGGGCCATCCGTAttgt                                                                         <  1:9169516‑M1/74‑1 (MQ=255)

                                                              tatggcagataacaaaccagagctacagcgtgggctggaagctcgacatatcgaactcatcgccctGGGGGGCACCATTGGCGTCGGCCTGTTTATGGGGGCCGCCAGTACCCTGAAATGGGCCGGGCCATCCGTAttgt                                                                         >  1:7055669‑M1/67‑140 (MQ=255)

                                                                tggcagataacaaaccagagctacagcgtgggctggatgatcgacatatcgaactcatcgccctGGGGGGCACCATTGGCGTCGGCCTGGTTATGGGGGCCGCCAGTACCCTGAAATGGGCCGGGCCATCCGTAttgttg                                                                       >  2:1370148‑M1/65‑140 (MQ=255)

                                                                tggcagataacaaaccagagctacagcgtgggctggaagctcgacatatcgaactcatcgccctGGGGGGCACCATTGGCGTCGGCCTGTTTATGGGGGCCGCCAGTACCCTGAAATGGGCCGGGCCATCCGTAttgttg                                                                       >  1:1104923‑M1/65‑140 (MQ=255)

                                                                  gcagataacaaaccagagctacagcgtgggctggaagctcgacatatcgaactcatcgccctGGGGGGCACCATTGGCGTCGGCCTGTTTATGGGGGCCGCCAGTACCCTGAAATGGGCCGGGCCATCCGTATTGTTGGc                                                                     >  1:4092681‑M1/63‑140 (MQ=255)

                                                                   cagataacaaaccagagctacagcgtgggctggaagctcgacatatcgaactcatcgccctGGGGGGCACCATTGGCGTCGGCCTGTTTATGGGGGCCGCCAGTACCCTGAAATGGGCCGGGCCATCCGTATTGTTGGcc                                                                    >  2:9235578‑M1/62‑140 (MQ=255)

                                                                     gataacaaaccagagctacagcgtgggctggaagctcgacatatcgaactcatcgccctGGGGGGCACCATTGGCGTCGGCCTGTTTATGGGGGCCGCCAGTACCCTGAAATggg                                                                                           >  2:8034816‑M1/60‑115 (MQ=255)

                                                                     gataacaaaccagagctacagcgtgggctggaagctcgacatatcgaactcatcgccctGGGGGGCACCATTGGCGTCGGCCTGTTTATGGGGGCCGCCAGTACCCTGAAATGGGCCGGGCCCTCCGGATTGTTGGCCta                                                                  <  1:6358192‑M1/81‑1 (MQ=255)

                                                                        aacaaaccagagctacagcgtgggctggaagctcgacatatcgaactcatcgccctGGGGGGCACCATTGGCGTCGGCCTGTTTATGGGGGCCGCCAGTACCCTGAAATGGGCCGGGCCATCCGTATTGTTGGCCTatat                                                               >  1:7589054‑M1/57‑140 (MQ=255)

                                                                                 gagctacagcgtgggctggaagctcgacatatcgaactcatcgccctGGGGGGCACCATTGGCGTCGGCCTGTTTATGGGGGCCGCCAGTACCCTGAAATGGGCCGGGCCATCCGTATTGTTGGCCTATATCATCGCCgg                                                      <  1:308458‑M1/93‑1 (MQ=255)

                                                                                 gagctacagcgtgggctggaagctcgacatatcgaactcatcgccctGGGGGGCACCATTGGCGTCGGCCTGTTTATGGGGGCCGCCAGTACCCTGAAATGGGCCGGGCCATCCGTATTGTTGGCCTATATCATCGCCgg                                                      >  1:7155062‑M1/48‑140 (MQ=255)

                                                                                  agctacagcgtgggctggaagctcgacatatcgaactcatcgccctGGGGGGCACCATTGGCGTCGGCCTGTTTATGGGGGCCGCCAGTACCCTGAAATGGGCCGGGCCATCCGTCTGGTTGGCCTATATCATCGCCggg                                                     <  1:6541945‑M1/94‑1 (MQ=255)

                                                                                  agctacagcgtgggctggaagctcgacatatcgaactcatcgccctGGGGGGCACCATTGGCGTCGGCCTGTTTATGGGGGCCGCCAGTACCCTGAAATGGGCCGGGCCATCCGTATTGTTGGCCTATATCATCGCCggg                                                     <  2:573958‑M1/94‑1 (MQ=255)

                                                                                  agctacagcgtgggctggaagctcgacatatcgaactcatcgccctGGGGGGCACCATTGGCGTCGGCCTGTTTATGGGGGCCGCCAGTACCCTGAAATGGGCCGGGCCATCCGTATTGTTGGCCTATATCATCGCCggg                                                     <  1:8968291‑M1/94‑1 (MQ=255)

                                                                                      acagcgtgggctggaagctcgacatatcgaactcatcgccctGGGGGGCACCATTGGCGTCGGCCTGTTTATGGGGGCCGCCAGTACCCTGAAATGGGCCGGGCCATCCGTATTGTTGGCCTATATCATCGCCGGGCTGt                                                 >  1:5111135‑M1/43‑140 (MQ=255)

                                                                                              ggctggaagctcgacatatcgaactcatcgccctGGGGGGCACCATTGGCGTCGGCCTGTTTATGGGGGCCGCCAGTACCCTGAAATGGGCCGGGCCATCCGTATTGTTGGCCTATATCATCGCCGGGCTGTTCGTCttt                                         <  1:1038361‑M1/106‑1 (MQ=255)

                                                                                                 tggaagctcgacatatcgaactcatcgccctGGGGGGCACCATTGGCGTCGGCCTGTTTATGGGGGCCGCCAGTACCCTGAAATGGGCCGTGCCATCAGTATTGTTGGCATATATCATCGCCGGGCTGTTCGTCTTTTtc                                      >  2:2913403‑M1/32‑140 (MQ=255)

                                                                                                 tggaagctcgacatatcgaactcatcgccctGGGGGGCACCATTGGCGTCGGCCTGTTTATGGGGGCCGCCAGTACCCTGAAATGGGCCGGGCCATCCGTATTGTTGGCCTATCTCATCGCCGGGCTGTTCGTCTTTTtc                                      >  1:8830738‑M1/32‑140 (MQ=255)

                                                                                                 tggaagctcgacatatcgaactcatcgccctGGGGGGCACCATTGGCGTCGGCCTGTTTATGGGGGCCGCCAGTACCCTGAAATGGGCCGGGCCATCCGTATTGTTGGCCTATATCATCGCCGGGCTGTTCGTCTTTTtc                                      >  2:7735378‑M1/32‑140 (MQ=255)

                                                                                                   gaagctcgacatatcgaactcatcgccctGGGGGGCACCATTGGCGTCGGCCTGTTTATGGGGGCCGCCAGTACCCTGAAATGGGCCGGGCCATCCGTATTGTTGGCCTATATCATCGCCGGGCTGTTCGTCTTTTtcat                                    >  2:7200029‑M1/30‑140 (MQ=255)

                                                                                                    aagctcgacatatcgaactcatcgccctGGGGGGCACCATTGGCGTCGGCCTGTTTATGGGGGCCGCCAGTACCCTGAAATGGGCCGGGCCATCCGTATTGTTGGCCTATATCATCGCCGGGCTGTTCGTCTTTTtcatc                                   <  2:1825651‑M1/112‑1 (MQ=255)

                                                                                                    aagctcgacatatcgaactcatcgccctGGGGGGCACCATTGGCGTCGGCCTGTTTATGGGGGCCGCCAGTACCCTGAAATGGGCCGGGCCATCCGTATTGTTGGCCTATATCATCGCCGGGCTGTTCGTCTTTTtcatc                                   <  2:7686297‑M1/112‑1 (MQ=255)

                                                                                                    aagctcgacatatcgaactcatcgccctGGGGGGCACCATTGGCGTCGGCCTGTTTATGGGGGCCGCCAGTACCCTGAAATGGGCCGGGCCATCCGTATTGTTGGCCTATATCATCGCCGGGCTGTTCGTCTTTTtcatc                                   >  1:5353870‑M1/29‑140 (MQ=255)

                                                                                                         cgacatatcgaactcatcgccctGGGGGGCACCATTGGCGTCGGCCTGTTTATGGGGGCCGCCAGTACCCTGAAATGGGCCGGGCCATCCGTATTGTTGGCCTATATCATCGCCGGGCTGTTCGTCTTTTTCATCATGCg                              <  2:1512272‑M1/117‑1 (MQ=255)

                                                                                                         cgacatatcgaactcatcgccctGGGGGGCACCATTGGCGTCGGCCTGTTTATGGGGGCCGCCAGTACCCTGAAATGGGCCGGGCCATCCGTATTGTTGGCCTATATCATCGCCGGGCTGTTCGTCTTTTTCATCATGCg                              <  1:424402‑M1/117‑1 (MQ=255)

                                                                                                            cagatcgaactcatcgccctGGGGGGCACCATTGGCGTCGGCCTGTTTATGGGGGCCGCCAGTACCCTGAAATGGGCCGGGCCATCCGTATTGTTGGCCTATATCATCGCCGGGCTGTTCGTCTTTTTCATCATGCGTTc                           >  2:4517038‑M1/21‑140 (MQ=255)

                                                                                                               atcgaactcatcgccctGGGGGGCACCATTGGCGTCGGCCTGTTTATGGGGGCCGCCAGTACCCTGAAATGGGCCGGGCCATCCGTATTGTTGGCCTATATCATCGCCGGGCTGTTCGTCTTTTTCATCATGCGTTCAAt                        >  2:5001691‑M1/18‑140 (MQ=255)

                                                                                                                  gaactcatcgccctGGGGGGCACCATTGGCGTCGGCCTGTTTATGGGGGCCGCCAGTACCCTGAAATGGGCCGGGCCATCCGTATTGTTGGCCTATATCATCGCCGGGCTGTTCGTCTTTTTCATCATGCGTTCAATggg                     <  1:3393215‑M1/126‑1 (MQ=255)

                                                                                                                    acacatcgccctGGGGGGCACCATTGGCGTCGGCCTGTTGATGGGGGCCGCCAGTACCCTGAAATTGGCCGGGCCATCCGTATTGTTGGCCTAGATCATCGCCGGGCTGTTCGTCTTTTTCATCATGCGTTCAATGGGCg                   >  2:208237‑M1/13‑140 (MQ=255)

                                                                                                                     ctcatcgccctGGGGGGCACCAGTGGCGTCGGCCTGTTTATGGGGGCCGCCAGTACCCTGAAATGGGCCGGGCCATCCGTATTGTTGGCCTATATCATCGCCGGGCTGTTCGTCTTTGTCATCATGCGTTCAATGGGCGa                  >  2:4002847‑M1/12‑140 (MQ=255)

                                                                                                                        atcgccctGGGGGGCACCATTGGCGTCGGCCTGTTTATGGGGGCCGCCAGTACCCTGAAATGGGCCGGGCCATCCGTATTGTTGGCCTATATCATCGCCGGGCTGTTCGTCTTTTTCATCATGCGTTCAATGGGCGAAAt               <  2:3216475‑M1/132‑1 (MQ=255)

                                                                                                                        atcgccctGGGGGGCACCATTGGCGTCGGCCTGTTTATGGGGGCCGCCAGTACCCTGAAATGGGCCGGGCCATCCGTATTGTTGGCCTATATCATCGCCGGGCTGTTCGTCTTTTTCATCATGAGTTCAATGGGCGAAAt               <  2:8379380‑M1/132‑1 (MQ=255)

                                                                                                                          cgccctGGGGGGCACCATTGGCGTCGGCCTGTTTATGGGGGCCGCCAGTACCCTGAAATGGGCCGGGCCATCCGTATTGTTGGCCTATATCATCGCCGGGCTGTTCGTGTTTTTCTTCATGCGTTCAATGGGCGAAAtgt             <  2:2385017‑M1/134‑1 (MQ=255)

                                                                                                                          cgccctGGGGGGCACCATTGGCGTCGGCCTGTTTATGGGGGCCGCCAGTACCCTGAAATGGGCCGGGCCATCCGTATTGTTGGCCTATATCATCGCCGGGCTGTTCGTCTTTTTCATCATGCGTTCAATGGGCGAAAtgt             <  2:6308227‑M1/134‑1 (MQ=255)

                                                                                                                            cctgggggg‑CACCATTGGCGTCGGCCGGTTTATGGGGGCCGCCAGTACCCTGAAATGGGCCGGGCCATCCGTATTGTTGGCCTATATCATCGCCGGGCTGTTCGTCTTTTTCATCATGCGTTCAATGGGCGAAAggttgt          >  2:4128537/4‑140 (MQ=255)

                                                                                                                               gggggg‑CACCATTGGCGTCGGCCTGTTTATGGGGGCCGCCAGTACCCTGAAATGGGCCGGGCCATCCGTATTGTTGGCCTATATCATCGCCGGGCTGTTCGTCTTTTTCATCATGCGTTCAATGGGCGAAATGTTGTTcc       >  2:2584339/1‑140 (MQ=255)

                                                                                                                                 GGGG‑CACCATTGGCGTCGGCCTGTTTATGGGGGCCGCCAGTACCCTGAAATGGGCCGGGCCATCCGTATTGTTGGCCTATATCATCGCCGGGCTGTTCGTCTTTTTCATCATGCGTTCAATGGGCGAAATGTTGTTCCTc     <  2:861048‑M1/140‑1 (MQ=255)

GATGTACCCATAACGATAACCGGTAACACCGGAAAGCATGCAAACACAACACGAGGATTTATGGCAGATAACAAACCAGAGCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCCTGGGGGGGG‑CACCATTGGCGTCGGCCTGTTTATGGGGGCCGCCAGTACCCTGAAATGGGCCGGGCCATCCGTATTGTTGGCCTATATCATCGCCGGGCTGTTCGTCTTTTTCATCATGCGTTCAATGGGCGAAATGTTGTTCCTCGAA  >  NZ_CP025268/3982747‑3983018

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`