Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0252682,233,2250TA25.6% 180.8 / 38.2 123intergenic (+125/+248)yohP/dusCsmall membrane protein YohP/tRNA dihydrouridine(16) synthase DusC
Reads supporting (aligned to +/- strand):  ref base T (41/49);  new base A (31/0);  total (73/50)
Fisher's exact test for biased strand distribution p-value = 4.17e-09
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

Only 100 of 126 total aligned reads displayed.

GATGATATTTTAAAATTAATTAATGTCATCAGGTCCGAAAATAACGAGAATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGC‑GCAGGGGGCGGCCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTAACATCCCTGTAAAACGCGCCCTCAGCCCACATCCATGTGG  >  NZ_CP025268/2233088‑2233364
                                                                                                                                          |                                                                                                                                           
gatgatATTTTAAAATTAATTAATGTCATCAGGTCCGAAAATAACGAGAATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGC‑GCAGGGGGCGGCCAATcg                                                                                                                                           >  2:4833410/1‑140 (MQ=255)
gatgatATTTTAAAATTAATTAATGTCATCAGGTCCGAAAATAACGAGAATATTTCAGTCTCTCATCCGGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGC‑GCAGGGGGGGGCCCATcg                                                                                                                                           >  1:7743523/1‑140 (MQ=255)
 atgatATTTTAAAATTAATTAATGTCATCAGGTCCGAAAATAACGAGAATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGC‑GCGGGGGGGGGCCAAAcgc                                                                                                                                          >  1:3654291/1‑140 (MQ=255)
     tatTTTAAAATTAATTAATGTCATCAGGTCCGAAAATAACGAGAATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGC‑GGCGGGGGGGGCCCAAcgccccc                                                                                                                                      >  1:6491459/1‑140 (MQ=255)
     tatTTTAAAATTAATTAATGTCATCAGGTCCGAAAATAACGAGAATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGC‑GCAGGGGGCGGCCAATcgccgcc                                                                                                                                      <  2:4597805/140‑1 (MQ=255)
     tatTTTAAAATTAATTAATGTCATCAGGTCCGAAAATAACGAGAATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGC‑GCAGGGGGCGGCCAAAcgccgcc                                                                                                                                      >  1:1942375/1‑140 (MQ=255)
             aattaattAATGTCATCAGGTCCTAAAATAACGAGAATATTTAAGACTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGC‑GCAGGGGGCGGCCAATCGCCGCCGCCCCCTg                                                                                                                              <  1:4220387/140‑1 (MQ=255)
               ttaattaaTGTCATCAGGTCCGAAAATAACGAGAATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGAATTGCTTCATATAATCACTGGCGCAAGGAGCGC‑GCAGGGGGCGGCCAATCGCCGCCGCCCCCTGCa                                                                                                                            <  2:84015/140‑1 (MQ=255)
                 aattaatGTCATCAGGTCCGAAAATAACGAGAATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGC‑GCAGGGGGCGGCCAAt                                                                                                                                             <  1:8413721/121‑1 (MQ=255)
                       tGTCATCAGGTCCGAAAATAACGAGAATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGC‑GCAGGGGGCGGCCAATCGCCGCCGCCCCCTGCACCCCCggg                                                                                                                    <  1:8934757/140‑1 (MQ=255)
                           atcaGGTCCGAAAATAACGAGAATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATACTCACTGGCGCAAGGAGCGC‑GGGGGGGGGGGCCAATCGCCGCCCCCCCCCGCACCCCCGGGctct                                                                                                                >  1:6555999/1‑140 (MQ=37)
                           atcaGGTCCGAAAATAACGAGAATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCTCAAGGAGCGC‑GCAGGGGGCGGCCAAACGCCGCCGCCCCCAGCACCCCCGGGcact                                                                                                                >  2:6112408/1‑140 (MQ=255)
                           atcaGGTCCGAAAATAACGAGAATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGCGGCGGGGGGGGGCCCAAC‑CCCCCCCCCCCCCCAcccccccgcgca                                                                                                                >  1:5488601/1‑133 (MQ=255)
                           atcaGGTCCGAAAATAACGAGAATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGC‑GGAGGGGGCGGGCAAAAGCCCCCGCCCCCCGCCCCCCCCGGcact                                                                                                                >  1:1787178/1‑140 (MQ=37)
                           atcaGGTCCGAAAATAACGAGAATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGC‑GCGGGGGGGGGCCAAAAGCCCCCCCCCCCAGCACCCCCCGGcaca                                                                                                                >  1:9325940/1‑137 (MQ=37)
                           atcaGGTCCGAAAATAACGAGAATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGC‑GCCGGGGGCGGCCAATCGCCGCCGCCCCCAGCACCCCCCGGctct                                                                                                                >  2:7221319/1‑140 (MQ=255)
                           atcaGGTCCGAAAATAACGAGAATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGC‑GCAGGGGGCGGCCAATCGCCGCCGCCCCCAGCACCCCCGGGcact                                                                                                                >  2:7221358/1‑140 (MQ=255)
                           atcaGGTCCGAAAATAACGAGAATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCAGATAAGCACTGGCGCAAGGAGCGC‑GCAGGGGGCGGCCAAAAGCCGCCGCCCCCAGCACCCCCCGGcacg                                                                                                                >  2:469507/1‑137 (MQ=37)
                           atcaGGTCCGAAAATAACGAGAATATTTCAGTCTCTCAGCCTGGTGCGCTCCTGTCATGTGCATTGCTTCATATAAGCACTGGCGCAAGGAGCGC‑GCAGGGGGCGGCCAATCGCCGCCGCCCCCTGCACCCCCGGGcgca                                                                                                                >  2:1880769/1‑137 (MQ=255)
                            tcaGGTCCGAAAATAACGAGAATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGC‑GCAGGGGGCGGCCAATCGCCGCCGCCCCCAGCACCCCCGGGCGCTg                                                                                                               >  2:2696612/1‑140 (MQ=255)
                                     aaaaTAACGAGAATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGC‑GCAGGGGGCGGCCAAACGCCGCCCCCCCCAGCCCCCCCCGGCTCTGGCGAACaaa                                                                                                      >  2:5376072/1‑140 (MQ=25)
                                       aaTAACGAGAATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGC‑GCAGGGGGCGGCCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGAACAAAAt                                                                                                    <  2:1380990/140‑1 (MQ=255)
                                          aaCGAGAATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGC‑GCAGGGGGCGGCCCCAACCCCCCCCCCCCCGCCCCCCCCCGCCCAGGCGCACaaaaaacc                                                                                                 >  1:2867670/1‑136 (MQ=25)
                                          aaCGAGAATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGC‑GCAGGGGGCGGCCAATCGCCGCCGCCCCCAGCACCCCCGGGCTCAGGCGAACAAAATcgc                                                                                                 >  2:1925989/1‑140 (MQ=255)
                                          aaCGAGAATATTTCAGTATCTCATCCTGTTGCGCTCCTGTCATGTGCATTGATTCATATAATCACTGGCGCAAGGAGCGC‑GCAGGGGGCGGCCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGAACAAAATcgc                                                                                                 <  1:1403306/140‑1 (MQ=255)
                                           aCGAGAATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGC‑GCCGGGGGCGGCCCCTCGCCGCCCCCCCCACCACCCCCCGGCCCAGGCGACCAAAATcgcc                                                                                                >  1:4063093/1‑140 (MQ=21)
                                           aCGAGAATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGC‑GCCGGGGGCGGCCAAACCCCCCCCCCCCCAGCAACCCCCCGCACATGCGAaaaaaaaaccc                                                                                                >  1:6894577/1‑135 (MQ=21)
                                           aCGAGAATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGC‑GCAGGGGGCGGCCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGAACAAAATcgcc                                                                                                <  2:8922287/140‑1 (MQ=255)
                                           aCGAGAATAGTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGC‑GCAGGGGGCGGCCAATCGCCGCCGCCCCCAGCCCCCCCGGGCACTGGCGAACaaaaaagcc                                                                                                >  2:1906554/1‑135 (MQ=21)
                                                aaTATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGC‑GCAGGGGGCGGCCAATAGCCCCCGCCCCCAGCACCCCCGGGCTCATGCGAACAAAAAAGCCGCGgc                                                                                           >  2:3730079/1‑140 (MQ=21)
                                                   atTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGC‑GCAGGGGGCGGCCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGcgcg                                                                                        <  2:53196/140‑1 (MQ=255)
                                                          tctctcATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGC‑GCAGGGGGCGGCCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCt                                                                                 <  1:5654989/140‑1 (MQ=255)
                                                          tctctcATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGC‑GCAAGGGGCGGCCAAAAGCCCCCCCCCCCAGCACCCCCCGGCACAGGCGAACAAAAACAACCCAGCGCCGTGccca                                                                                 >  2:1445228/1‑139 (MQ=18)
                                                            tctcATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATCAGCACTGGCGCAAGGAGCGC‑GGACGGGGGGGCCAATCCCCGCCGCCCCCGGCACCCCCGGGCTCTGGCGGAGCCAATCACCGCTGCGCGGTGCCCACg                                                                               >  2:6403263/1‑140 (MQ=17)
                                                              tcATCCTGTTGCGCTCCTGTCATGTGCATTTCTTCATATAATCCCTGGCGCAAGGAGCGC‑GCAGGGGGCGGCCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCGCGGc                                                                             <  2:7157818/140‑1 (MQ=255)
                                                              tcATCCTGTTGCGCTCCTGTCATGTGCATTGCTACATATAATCACTGGCGCAAGGAGCGC‑GCAGGGGGCGGCCAAACGCCGCCCCCCCCAGCACCCCCGGGCACAGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGc                                                                             >  2:5119664/1‑140 (MQ=17)
                                                               cATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGC‑GCAGGGGGCGGCCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCt                                                                            >  2:7504288/1‑140 (MQ=255)
                                                               cATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGC‑GCAGGGGGCGGCCAAACGCCCCCGCCCCCAGCACCCCCCGGCACACGCGAACAAAAACGCCCCAGCGCGGTGCCCACGGCt                                                                            >  2:7504153/1‑140 (MQ=18)
                                                               cATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGC‑GCACGGGGCGGCCAATACCCGCCCCCCCCATCACCCCCCGGCGATGGCGAACAAAAAACCCCCAGCGCGGGGcccaccgcg                                                                            >  2:4405241/1‑134 (MQ=18)
                                                                aTCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGC‑GCAGGGGGCGGCCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCtt                                                                           <  2:8893729/140‑1 (MQ=255)
                                                                aTCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGC‑GCAGGGGGCGGCCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCtt                                                                           <  1:354301/140‑1 (MQ=255)
                                                                    tGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGC‑GCAGGGGGCGGCCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATcc                                                                       <  2:6633273/140‑1 (MQ=255)
                                                                    tGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGC‑GCAGGGGGCGGCCAATCGCCGCCGCCCCCTGCACCCCCGGGCACTGGCGAACAAAATCGCCGCTTCGCGGTGCCCACGGCTTAtac                                                                       >  2:9125553/1‑138 (MQ=255)
                                                                      ttGCGCTCCTGTCATGTGCATTGCTTCATCTAATCACTGGCGCAAGGAGCGC‑GCCGGGGGCGGCCCAAGGGCGCCGCCCCCAGCACCCCCCGGCTCTTGGGAACAAAAAACCCCCGGCGCGGTGCCCTCCGCCTATcccg                                                                     >  2:2512540/1‑139 (MQ=18)
                                                                      ttGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGC‑GCATGGGGCGGCCAATCGCCGCCGCCCCCTGCACCCCCCGGCTCTGGCGAACAAAATCGCCGCAGCGCGGTGCCCTCGGCATATacca                                                                     >  2:7874169/1‑139 (MQ=18)
                                                                      ttGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGC‑GCAGGGGGGGGCCCCACCCCCCCCCCCCCACCACCCCCCCGCACAGGCGAACAAAAAAACCCCCGCGCCGGGCCCCCGGCATaaacca                                                                     >  2:2188357/1‑135 (MQ=12)
                                                                      ttGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGC‑GCAGGGGGGGGCCCAAAGCCGCCCCCCCCAGCACCCCCCGGCTCTGGCGAACAAAAAAGCCGCGGCGCGGTGGCCTCGGCTTATccca                                                                     >  2:1460644/1‑139 (MQ=18)
                                                                          gcTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGC‑GCATGGGGCGGCCAATCGCCGCCGCCCCCTGCACCCCCGGGCTATGGCGAACAAAATAGCCGCTGCGCCGTGCCCTCGGCATATCCCTTACg                                                                 >  2:6851377/1‑140 (MQ=18)
                                                                          gcTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGC‑GCAGGGGGCGGCCAATCGCCGCCGCCCCCAGCACCCCCGGGCTCTGGCGAACAAAAACGCCGCTGCGCGGTGCCCTCGGCATATCCCTTACg                                                                 >  2:8786752/1‑140 (MQ=255)
                                                                           cTCCTGTCATGTGCATTGCTTCATCTAATCACTGGCGCAAGGAGCGC‑GGATGGGGCGGCCAAAAGCCGCCGCCCCCATCACCCCCCGGCACATGCGAAAAAAAACGCCCCATCGCGGTGGCCGCGGCTTATACCATaacg                                                                >  2:5822310/1‑137 (MQ=12)
                                                                              cTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGC‑GCAGGGGGCGGCCCATCGCCGCCGCCCCCGGCACCCCCGGGCTCTGGCGCACAAAATCGCCGCTGCGCGGGTCCCACGGCTTATACCAGAAGGCTa                                                             >  2:6404782/1‑140 (MQ=17)
                                                                                      tgCATTGCTTCATATAATCACTGGCGCAAGGAGCGC‑GCCGGGGGCGGCCAAACGCCGCCCCCCCCAGCACCCCCCGGCTCTGGCGAACAAAAAACCCCCAGCGCGGTGGCCCCCGCAGATCCCATAAGGCAAccccggcg                                                     >  2:5470792/1‑134 (MQ=9)
                                                                                      tgCATTGCTTCATATAATCACTGGCGCAAGGAGCGC‑GCCGGGGGCGGCCAAACGCCCCCGCCCCCCGCCACCCCCCGCACAGGCGAACAAAAAACCCGCAGCCCGGTGCCCACGGCATAAACCATACGGCAAACGGGTCg                                                     >  2:6365817/1‑140 (MQ=9)
                                                                                      tgCATTGCTTCATATAATCACTGGCGCAAGGAGCGC‑GCAGGGGGCGGCCAATCGCCGCCGCCCCCGGCCCCCCCGGGCTCTGGCGAACAAAAACGCCGCGGCGCGGTGCCCGCGGCTTATCCCATAAGGCTGCCCGGTCg                                                     >  2:1782353/1‑140 (MQ=14)
                                                                                        cATTGCTTCATATAATCACTGGCGCAAGGAGCGC‑GCAGGGGGCGGCCCAAAGCCCCCCCCCCCTTCACCCCCCGGCTCTGGCGAACAAAATAGCCCCTGCGCGGGGCCCGCGGGTTATCCCTTTCCGCTACCGGGGCggg                                                   >  2:6372351/1‑140 (MQ=14)
                                                                                        cATTGCTTCATATAATCACTGGCGCAAGGAGCGC‑GCAGGGGGCGGCCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCggg                                                   <  1:5851722/140‑1 (MQ=255)
                                                                                        cATTGCTTCATATAATCACTGGCGCAAGGAGCGC‑GCAGGGGGCGGCCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCggg                                                   <  2:6034012/140‑1 (MQ=255)
                                                                                          ttGCTTCATATAATCACTGGCGCAAGGAGCGC‑GCCAGGGGGGGGCAATAGCCGCCCCCCCCAGCACCCCCGGGCTCTGGCGAAACAAAAAGCCGCTGCGCGGTGCCCACGGCATATACCATACGGCTAACCGGTCGGgcg                                                 >  2:699495/1‑140 (MQ=14)
                                                                                          ttGCTTCATATAACCACTGGCGCAAGGAGCGC‑GCAGGGGGCGGCCACTCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCGGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGgcg                                                 <  2:2201569/140‑1 (MQ=255)
                                                                                           tGCTTCATATAATCACTGGCGCAAGGAGCGCGGCGGGGGGGGGCCAA‑AACCCCCCCCCCCCGCACCCCCCGGCTCTGGCGCAAAAAAACACCGCTGCGCGGGGGCCGCGGCGTATTCCCAAAGGCTACCCGGGGGGgcgc                                                >  2:2177641/1‑140 (MQ=255)
                                                                                           tGCTTCATATAATCACTGGCGCAAGGAGCGC‑GCCGGGGGCGGCCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGgcgc                                                <  1:1099782/140‑1 (MQ=255)
                                                                                           tGCTTCATATAATCACTGGCGCAAGGAGCGC‑GCAGGGGGCGGCCCATCGCCCCCGCCCCCTGCCCCCCCCGGCAAAGGCGAACAAACACCCCGCTGCGCGGTGCCCGCGGCTTATACCCTACCGCGACCCGGGCGGgcgc                                                >  2:8543930/1‑140 (MQ=14)
                                                                                           tGCTTCATATAATCACTGGCGCAAGGAGCGC‑GCAGGGGGCGGCCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGgcgc                                                <  1:7961640/140‑1 (MQ=255)
                                                                                           tGCTTCATATAATCACTGGCGCAAGGAGCGC‑GCAGGGGGCGGCCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGgcgc                                                <  1:2375394/140‑1 (MQ=255)
                                                                                             cTTCATATAATCACTGGCGCAAGGAGCGC‑GCAGGGGGCGGCCAAAAGCCCCCCCCCCCAGCACCCCCCGGCACATGCGAACAAAAAACCCGCGGCGCCGTGCCCACGGCGTATCCCCAAAGGCTAACCGGTCGGGCGCGa                                              >  2:4890204/1‑140 (MQ=9)
                                                                                                cATATCATCCCTGGCGCAAGGAGCGC‑GCAGGGGGCGGCCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGt                                           <  1:8871427/140‑1 (MQ=25)
                                                                                                cATATAATCACTGGCGCACGGAGCGC‑GCAGGGGGCGGCCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGt                                           <  1:188458/140‑1 (MQ=255)
                                                                                                cATATAATCACTGGCGCAAGGAGCGC‑GCAGGGGGCGGCCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGt                                           <  1:188633/140‑1 (MQ=39)
                                                                                                cATATAATCACTGGCGCAAGGAGCGC‑GCAGGGGGCGGCCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGt                                           <  2:9392425/140‑1 (MQ=39)
                                                                                                cATATAATCACTGGCGCAAGGAGCGC‑GCAGGGGGCGGCCAATAGCCCCCGCCCCCAGCACCCCCCGGCTCAGGCGAACAAAAAAGCCCCGGCGCGGTGCCCACCGCATATCCCATACCGCAACCCGGTCGGGCGCGACGt                                           >  2:3028404/1‑140 (MQ=14)
                                                                                                cATATAATCACTGGCGCAAGGAGCGC‑GCAGGGGGCGGCCAAAAGCCGCCGCCCCCTGCACCCCCCGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCACGGCATATCCCATACGGCAACCCGGTCGGGCGCGAGGt                                           >  2:3028533/1‑140 (MQ=14)
                                                                                                  tataATCACTGGCGCATGGAGCGC‑GATGGGGGCGGCCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTaa                                         <  1:2847370/140‑1 (MQ=255)
                                                                                                     aaTCACTGGCGCAAGGAGCGC‑GCAGGGGGCGGCCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTAACAt                                      <  1:7004534/140‑1 (MQ=34)
                                                                                                     aaTCACTGGCGCAAGGAGCGC‑GCAGGGGGCGGCCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTAACAt                                      <  2:311971/140‑1 (MQ=34)
                                                                                                     aaTCACTGGCGCAAGGAGCGC‑GCAGGGGGCGGCCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTAACAt                                      <  1:5516360/140‑1 (MQ=34)
                                                                                                     aaTCACTGGCGCAAGGAGCGC‑GCAGGGGGCGGCCAATAGCCGCCGCCCCCTGCAACCCCGGGCTCTGGCGAACAAAATCCGCCCAGCGCGGTGCCCACGGCATATCCCGTACGGCTACCCGGTCGGGCGCGAGGTAACAt                                      >  2:7059965/1‑140 (MQ=11)
                                                                                                       tCACTGGCGCAAGGAGCGC‑GGAGGGGGCGGCCCAAAACCCCCGCCCCCAGCACCCCCCGGCTCATGCGGAAAAAAAAACCCCAGCGCGGTGCCCACCGCCTTTACCATAAGGCGAACCGGTAGGGCGCGAGGTAACaaac                                    >  2:3336824/1‑137 (MQ=255)
                                                                                                       tCACTGGCGCAAGGAGCGC‑GCCGGGGGCGGCCAATCGCCCCCGCCCCCAGCACCCCCCGGCGCAGGCCAACAAAATCGCCGCGGCGCGGGGCCCTCGGCGTATACCTTACGGCTTCCGGGTCGGGCGCGAGGTaaactcc                                    >  2:6714505/1‑135 (MQ=11)
                                                                                                       tCACTGGCGCAAGGAGCGC‑GCAGGGGGGGGGCAATAGCCGCCGCCCCCAGCACCCCCCGGCTCAGGCGCACAAAAACGCCGCGGCGCGGGGCCCGAGGCATATCCCGTACGGCTACCGGGTAGGGCGCGAGGTAAccacc                                    >  2:3977735/1‑136 (MQ=11)
                                                                                                       tCACTGGCGCAAGGAGCGC‑GCAGGGGGCGGCCCAACACCCCCCCCCCCCGCCCACCCCGGCGCAGGCGACCAAAAACGCCGCGTCGCGGTGCCCGCCGCATAACCCATACGGCTACCCGGGCCGGCGCGAGGTAACaaac                                    >  2:1650785/1‑137 (MQ=2)
                                                                                                       tCACTGGCGCAAGGAGCGC‑GCAGGGGGCGGCCCAAAGCCCCCGCCCCCCGCCCCCCCCGGCACTGGCGAACAAAAACGCCCCGGCGCGGTGCCCACGGCGTATACCCTACCGCGACCCGGTCGGGCGCGCGGGAACAtac                                    >  2:4785982/1‑138 (MQ=2)
                                                                                                       tCACTGGCGCAAGGAGCGC‑GCAGGGGGCGGCCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTAACATcc                                    <  2:6768586/140‑1 (MQ=33)
                                                                                                           tGGCGCAAGGAGCGC‑GCAGGGGGGGGCCCAAAGCCGCCGCCCCCAGCAACCCCGGGCTCTGTCGAACAAAAAAGCCCCGTCGCGGTGCCCTAGGCATATACCATAAGGCAACCGGGTCGGGCGCGAGGTAACCTCCCAGt                                >  2:833676/1‑140 (MQ=2)
                                                                                                                  aGGAGCGC‑GCAGGGGGCGGCCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTAACATCCCTGTAAAAcgc                         <  2:8796130/140‑1 (MQ=32)
                                                                                                                       cgc‑gcAGGGGGCGGCCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTAACATCCCTGTAAAACGCGCCCt                    <  2:1942375/140‑1 (MQ=32)
                                                                                                                       cgc‑gcAGGGGGCGGCCAAGCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTAACATCCCTGTAAAACGCGCCCt                    <  2:6094488/140‑1 (MQ=17)
                                                                                                                        gc‑gcAGGGGGCGGCCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTAACATCCCTGTAAAACGCGCCCTc                   <  2:7434898/140‑1 (MQ=32)
                                                                                                                         c‑gcAGGGGGCGGCCAATAGCCGCCGCCCCCCGCACCCCCCGGCTCTGGCGAACAAAAAAGCCGCAGCGCGGGGGCCACGGCGTAAAACATACGGCTACCCGGTCGGGCGCGAGGTAACATCCCAGTAAAAAGCGCCCTCa                  >  2:7264353/1‑140 (MQ=11)
                                                                                                                           gcAGGGGGCGGCCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTAACATCCCTGTAAAACGCGCCCTCAg                 <  1:2822178/140‑1 (MQ=32)
                                                                                                                             aGGGGGCGGCCACTCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTAACATCCCTGTAAAACGCGCCCTCAGcc               <  2:7283641/140‑1 (MQ=17)
                                                                                                                              gggggCGGCCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTAACATCCCTGTAAAACGCGCCCTCAGccc              <  1:7504288/140‑1 (MQ=32)
                                                                                                                                 ggcggcCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTAACATCCCTGTAAAACGCGCCCTCAGCCcaca           <  2:2613603/140‑1 (MQ=32)
                                                                                                                                   cggcCAATCGCCGCCGCCCCCAGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTAACATCCCTGTAAAACGCGCCCTCAGCCCACATc         >  2:761680/1‑140 (MQ=17)
                                                                                                                                    ggcCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTAACATCCCTGTAAAACGCGCCCTCAGcc               <  1:8918208/133‑1 (MQ=32)
                                                                                                                                     gcCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTAACATCCCTGTAAAACGCGCCCTCAGCCCACATCCa       >  1:3033829/1‑140 (MQ=32)
                                                                                                                                      ccAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTAACATCCCTGTAAAAcg                          <  2:8147329/120‑1 (MQ=32)
                                                                                                                                      ccAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTAACATCCCTGTAAAACGCGCCCTCAGCCCACATCCAt      <  2:1145125/140‑1 (MQ=32)
                                                                                                                                      ccAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTAACATCCCTGTAAAACGCGCCCTCAGCCCACATCCAt      <  2:1145176/140‑1 (MQ=32)
                                                                                                                                         aTCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTACCATCCCTGTAAAACGCGCCCTCAGCCCACATCCAtgtg   >  2:1550522/1‑140 (MQ=17)
                                                                                                                                         aTCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTAACATCCCTGTAAAACGCGCCCTCAGCCCACATCCAtgtg   >  1:1310290/1‑140 (MQ=32)
                                                                                                                                          |                                                                                                                                           
GATGATATTTTAAAATTAATTAATGTCATCAGGTCCGAAAATAACGAGAATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGC‑GCAGGGGGCGGCCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTAACATCCCTGTAAAACGCGCCCTCAGCCCACATCCATGTGG  >  NZ_CP025268/2233088‑2233364

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑