Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0252682,233,2480GC25.2% 291.2 / 61.7 147intergenic (+148/+225)yohP/dusCsmall membrane protein YohP/tRNA dihydrouridine(16) synthase DusC
Reads supporting (aligned to +/- strand):  ref base G (57/53);  new base C (37/0);  total (94/53)
Fisher's exact test for biased strand distribution p-value = 3.65e-09
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

Only 100 of 149 total aligned reads displayed.

TGTCATCAGGTCCGAAAATAACGAGAATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGC‑GCAGGGGGCGGCCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTAACATCCCTGTAAAACGCGCCCTCAGCCCACATCCATGTGGGCTGCCCCGGCCTTCAGGGAAC  >  NZ_CP025268/2233111‑2233386
                                                                                                                                          |                                                                                                                                          
tGTCATCAGGTCCGAAAATAACGAGAATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGC‑GCAGGGGGCGGCCAATCGCCGCCGCCCCCTGCACCCCCggg                                                                                                                                          <  1:8934757/140‑1 (MQ=255)
    atcaGGTCCGAAAATAACGAGAATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCTCAAGGAGCGC‑GCAGGGGGCGGCCAAACGCCGCCGCCCCCAGCACCCCCGGGcact                                                                                                                                      >  2:6112408/1‑140 (MQ=255)
    atcaGGTCCGAAAATAACGAGAATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGC‑GGAGGGGGCGGGCAAAAGCCCCCGCCCCCCGCCCCCCCCGGcact                                                                                                                                      >  1:1787178/1‑140 (MQ=37)
    atcaGGTCCGAAAATAACGAGAATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGC‑GCGGGGGGGGGCCAAAAGCCCCCCCCCCCAGCACCCCCCGGcaca                                                                                                                                      >  1:9325940/1‑137 (MQ=37)
    atcaGGTCCGAAAATAACGAGAATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGC‑GCCGGGGGCGGCCAATCGCCGCCGCCCCCAGCACCCCCCGGctct                                                                                                                                      >  2:7221319/1‑140 (MQ=255)
    atcaGGTCCGAAAATAACGAGAATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGC‑GCAGGGGGCGGCCAATCGCCGCCGCCCCCAGCACCCCCGGGcact                                                                                                                                      >  2:7221358/1‑140 (MQ=255)
    atcaGGTCCGAAAATAACGAGAATATTTCAGTCTCTCAGCCTGGTGCGCTCCTGTCATGTGCATTGCTTCATATAAGCACTGGCGCAAGGAGCGC‑GCAGGGGGCGGCCAATCGCCGCCGCCCCCTGCACCCCCGGGcgca                                                                                                                                      >  2:1880769/1‑137 (MQ=255)
              aaaaTAACGAGAATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGC‑GCAGGGGGCGGCCAAACGCCGCCCCCCCCAGCCCCCCCCGGCTCTGGCGAACaaa                                                                                                                            >  2:5376072/1‑140 (MQ=25)
                aaTAACGAGAATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGC‑GCAGGGGGCGGCCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGAACAAAAt                                                                                                                          <  2:1380990/140‑1 (MQ=255)
                   aaCGAGAATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGC‑GCAGGGGGCGGCCCCAACCCCCCCCCCCCCGCCCCCCCCCGCCCAGGCGCACaaaaaacc                                                                                                                       >  1:2867670/1‑136 (MQ=25)
                   aaCGAGAATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGC‑GCAGGGGGCGGCCAATCGCCGCCGCCCCCAGCACCCCCGGGCTCAGGCGAACAAAATcgc                                                                                                                       >  2:1925989/1‑140 (MQ=255)
                    aCGAGAATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGC‑GCCGGGGGCGGCCAAACCCCCCCCCCCCCAGCAACCCCCCGCACATGCGAaaaaaaaaccc                                                                                                                      >  1:6894577/1‑135 (MQ=21)
                    aCGAGAATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGC‑GCAGGGGGCGGCCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGAACAAAATcgcc                                                                                                                      <  2:8922287/140‑1 (MQ=255)
                         aaTATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGC‑GCAGGGGGCGGCCAATAGCCCCCGCCCCCAGCACCCCCGGGCTCATGCGAACAAAAAAGCCGCGgc                                                                                                                 >  2:3730079/1‑140 (MQ=21)
                                   tctctcATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGC‑GCAAGGGGCGGCCAAAAGCCCCCCCCCCCAGCACCCCCCGGCACAGGCGAACAAAAACAACCCAGCGCCGTGccca                                                                                                       >  2:1445228/1‑139 (MQ=18)
                                      ctcATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGC‑GCAGGGGGCGGCCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCgg                                                                                                    <  2:5593190/140‑1 (MQ=255)
                                       tcATCCTGTTGCGCTCCTGTCATGTGCATTTCTTCATATAATCCCTGGCGCAAGGAGCGC‑GCAGGGGGCGGCCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCGCGGc                                                                                                   <  2:7157818/140‑1 (MQ=255)
                                       tcATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGC‑GCAGGGGGCGGCCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCGGCGCGGTGCCCTCGGc                                                                                                   >  2:7831293/1‑140 (MQ=255)
                                       tcATCCTGTTGCGCTCCTGTCATGTGCATTGCTACATATAATCACTGGCGCAAGGAGCGC‑GCAGGGGGCGGCCAAACGCCGCCCCCCCCAGCACCCCCGGGCACAGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGc                                                                                                   >  2:5119664/1‑140 (MQ=17)
                                        cATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGC‑GCAGGGGGCGGCCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCt                                                                                                  >  2:7504288/1‑140 (MQ=255)
                                        cATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGC‑GCAGGGGGCGGCCAAACGCCCCCGCCCCCAGCACCCCCCGGCACACGCGAACAAAAACGCCCCAGCGCGGTGCCCACGGCt                                                                                                  >  2:7504153/1‑140 (MQ=18)
                                        cATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGC‑GCACGGGGCGGCCAATACCCGCCCCCCCCATCACCCCCCGGCGATGGCGAACAAAAAACCCCCAGCGCGGGGcccaccgcg                                                                                                  >  2:4405241/1‑134 (MQ=18)
                                         aTCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGC‑GCAGGGGGCGGCCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCtt                                                                                                 <  1:354301/140‑1 (MQ=255)
                                         aTCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGC‑GCAGGGGGCGGCCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCtt                                                                                                 <  2:8893729/140‑1 (MQ=255)
                                             tGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGC‑GCAGGGGGCGGCCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATcc                                                                                             <  2:6633273/140‑1 (MQ=255)
                                               ttGCGCTCCTGTCATGTGCATTGCTTCATCTAATCACTGGCGCAAGGAGCGC‑GCCGGGGGCGGCCCAAGGGCGCCGCCCCCAGCACCCCCCGGCTCTTGGGAACAAAAAACCCCCGGCGCGGTGCCCTCCGCCTATcccg                                                                                           >  2:2512540/1‑139 (MQ=18)
                                               ttGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGC‑GCATGGGGCGGCCAATCGCCGCCGCCCCCTGCACCCCCCGGCTCTGGCGAACAAAATCGCCGCAGCGCGGTGCCCTCGGCATATacca                                                                                           >  2:7874169/1‑139 (MQ=18)
                                               ttGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGC‑GCAGGGGGGGGCCCCACCCCCCCCCCCCCACCACCCCCCCGCACAGGCGAACAAAAAAACCCCCGCGCCGGGCCCCCGGCATaaacca                                                                                           >  2:2188357/1‑135 (MQ=12)
                                               ttGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGC‑GCAGGGGGGGGCCCAAAGCCGCCCCCCCCAGCACCCCCCGGCTCTGGCGAACAAAAAAGCCGCGGCGCGGTGGCCTCGGCTTATccca                                                                                           >  2:1460644/1‑139 (MQ=18)
                                                       cTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGC‑GCAGGGGGCGGCCCATCGCCGCCGCCCCCGGCACCCCCGGGCTCTGGCGCACAAAATCGCCGCTGCGCGGGTCCCACGGCTTATACCAGAAGGCTa                                                                                   >  2:6404782/1‑140 (MQ=17)
                                                         gTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGC‑GCCGGGGGCGGCCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTAcc                                                                                 <  1:337547/140‑1 (MQ=255)
                                                               tgCATTGCTTCATATAATCACTGGCGCAAGGAGCGC‑GCCGGGGGCGGCCAAACGCCCCCGCCCCCCGCCACCCCCCGCACAGGCGAACAAAAAACCCGCAGCCCGGTGCCCACGGCATAAACCATACGGCAAACGGGTCg                                                                           >  2:6365817/1‑140 (MQ=9)
                                                               tgCATTGCTTCATATAATCACTGGCGCAAGGAGCGC‑GCAGGGGGCGGCCCATCCCCGCCCCCCCCAGCAACCCCCGGCTCAGGGGAACCAAATCTCCCCTGCGCGGGGCCCACCGCGTATCCCCAACCGCTAACCGGTCg                                                                           >  2:3701248/1‑140 (MQ=9)
                                                                 cATTGCTTCATATAATCACTGGCGCAAGGAGCGC‑GCAGGGGGCGGCCCAAAGCCCCCCCCCCCTTCACCCCCCGGCTCTGGCGAACAAAATAGCCCCTGCGCGGGGCCCGCGGGTTATCCCTTTCCGCTACCGGGGCggg                                                                         >  2:6372351/1‑140 (MQ=14)
                                                                 cATTGCTTCATATAATCACTGGCGCAAGGAGCGC‑GCAGGGGGCGGCCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCggg                                                                         <  2:6034012/140‑1 (MQ=255)
                                                                 cATTGCTTCATATAATCACTGGCGCAAGGAGCGC‑GCAGGGGGCGGCCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCggg                                                                         <  1:5851722/140‑1 (MQ=255)
                                                                   ttGCTTCATTTAATCACTGGCGAAAGGAGCGC‑GCAGGGGGCGGCCTATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGAACAAAATCGCCGATGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGgcg                                                                       <  2:1660546/140‑1 (MQ=255)
                                                                   ttGCTTCATATAATCACTGGCGCAAGGAGCGC‑GCCAGGGGGGGGCAATAGCCGCCCCCCCCAGCACCCCCGGGCTCTGGCGAAACAAAAAGCCGCTGCGCGGTGCCCACGGCATATACCATACGGCTAACCGGTCGGgcg                                                                       >  2:699495/1‑140 (MQ=14)
                                                                   ttGCTTAATTTACTCCCTGGCGCTTGGTGCGG‑GCTGGGGGCGGCCATTCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGGGCCCTCTGCTTATCCCTTACGGCTACCGGGTCGGgcg                                                                       <  1:429019/140‑1 (MQ=11)
                                                                    tGCTTCATATAATCACTGGCGCAAGGAGCGCGGCGGGGGGGGGCCAA‑AACCCCCCCCCCCCGCACCCCCCGGCTCTGGCGCAAAAAAACACCGCTGCGCGGGGGCCGCGGCGTATTCCCAAAGGCTACCCGGGGGGgcgc                                                                      >  2:2177641/1‑140 (MQ=255)
                                                                    tGCTTCATATAATCACTGGCGCAAGGAGCGC‑GCCGGGGGCGGCCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGgcgc                                                                      <  1:1099782/140‑1 (MQ=255)
                                                                    tGCTTCATATAATCACTGGCGCAAGGAGCGC‑GCAGGGGGCGGCCCATCGCCCCCGCCCCCTGCCCCCCCCGGCAAAGGCGAACAAACACCCCGCTGCGCGGTGCCCGCGGCTTATACCCTACCGCGACCCGGGCGGgcgc                                                                      >  2:8543930/1‑140 (MQ=14)
                                                                    tGCTTCATATAATCACTGGCGCAAGGAGCGC‑GCAGGGGGCGGCCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGgcgc                                                                      <  1:7961640/140‑1 (MQ=255)
                                                                    tGCTTCATATAATCACTGGCGCAAGGAGCGC‑GCAGGGGGCGGCCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGgcgc                                                                      <  1:2433412/140‑1 (MQ=255)
                                                                    tGCTTCATATAATCACTGGCGCAAGGAGCGC‑GCAGGGGGCGGCCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGgcgc                                                                      <  1:2375394/140‑1 (MQ=255)
                                                                         cATATAATCACTGGCGCAAGGAGCGC‑GCAGGGGGCGGCCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGt                                                                 <  2:9392425/140‑1 (MQ=39)
                                                                         cATATAATCACTGGCGCAAGGAGCGC‑GCAGGGGGCGGCCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGt                                                                 <  1:188633/140‑1 (MQ=39)
                                                                         cATATAATCACTGGCGCAAGGAGCGC‑GCAGGGGGCGGCCAATAGCCCCCGCCCCCAGCACCCCCCGGCTCAGGCGAACAAAAAAGCCCCGGCGCGGTGCCCACCGCATATCCCATACCGCAACCCGGTCGGGCGCGACGt                                                                 >  2:3028404/1‑140 (MQ=14)
                                                                           tataATCACTGGCGCATGGAGCGC‑GATGGGGGCGGCCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTaa                                                               <  1:2847370/140‑1 (MQ=255)
                                                                           tataATCACTGGCGCAAGGAGCGC‑GCAGGGGGCGGCCAAACGCCGCCGCCCCCAGCCCCCCCCGGCTCTGGCGCACAAAAAACCCGCGGCGCGGTGGCCACCGCTTATCCCTTACGGCTACCGGGTCGGGCGCGCGGGaa                                                               >  2:7414384/1‑140 (MQ=11)
                                                                              aaTCACTGGCGCAAGGAGCGC‑GCAGGGGGCGGCCCAAAGCCCCCCCCCCCAGCACCCCCCGGCACTGGCGAACAAAAAAGCCCCAGCGCCGTGCCCACCGCTTAAACCAAAACGCAACCCGGGCCGGCGCGAGGGAACaa                                                            >  2:2822178/1‑139 (MQ=2)
                                                                              aaTCACTGGCGCAAGGAGCGC‑GCAGGGGGCGGCCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTAACAt                                                            <  1:7004534/140‑1 (MQ=34)
                                                                              aaTCACTGGCGCAAGGAGCGC‑GCAGGGGGCGGCCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTAACAt                                                            <  2:311971/140‑1 (MQ=34)
                                                                              aaTCACTGGCGCAAGGAGCGC‑GCAGGGGGCGGCCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTAACAt                                                            <  1:5516360/140‑1 (MQ=34)
                                                                              aaTCACTGGCGCAAGGAGCGC‑GCAGGGGGCGGCCAATAGCCGCCGCCCCCTGCAACCCCGGGCTCTGGCGAACAAAATCCGCCCAGCGCGGTGCCCACGGCATATCCCGTACGGCTACCCGGTCGGGCGCGAGGTAACAt                                                            >  2:7059965/1‑140 (MQ=11)
                                                                                tCACTGGCGCAAGGAGCGC‑GGAGGGGGCGGCCCAAAACCCCCGCCCCCAGCACCCCCCGGCTCATGCGGAAAAAAAAACCCCAGCGCGGTGCCCACCGCCTTTACCATAAGGCGAACCGGTAGGGCGCGAGGTAACaaac                                                          >  2:3336824/1‑137 (MQ=255)
                                                                                tCACTGGCGCAAGGAGCGC‑GCAGGGGGCGGCCCAACACCCCCCCCCCCCGCCCACCCCGGCGCAGGCGACCAAAAACGCCGCGTCGCGGTGCCCGCCGCATAACCCATACGGCTACCCGGGCCGGCGCGAGGTAACaaac                                                          >  2:1650785/1‑137 (MQ=2)
                                                                                tCACTGGCGCAAGGAGCGC‑GCAGGGGGCGGCCCAAAGCCCCCGCCCCCCGCCCCCCCCGGCACTGGCGAACAAAAACGCCCCGGCGCGGTGCCCACGGCGTATACCCTACCGCGACCCGGTCGGGCGCGCGGGAACAtac                                                          >  2:4785982/1‑138 (MQ=2)
                                                                                tCACTGGCGCAAGGAGCGC‑GCAGGGGGCGGCCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTAACATcc                                                          <  2:6768586/140‑1 (MQ=33)
                                                                                tCACTGGCGCAAGGAGCGC‑GCAGGGGGCGGCCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTAACATcc                                                          <  2:6768661/140‑1 (MQ=33)
                                                                                tCACTGGCGCAAGGAGCGC‑GCAGGGGGCGGCCAATAGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGAACAAAATAGCCGCGGCGCGGTGCCCACGGCTTATACCTTACGGCTACCGGGTCGGGCGCGAGGTAACATcc                                                          >  2:5084517/1‑140 (MQ=11)
                                                                                    tGGCGCAAGGAGCGC‑GCAGGGGGGGGCCCAAAGCCGCCGCCCCCAGCAACCCCGGGCTCTGTCGAACAAAAAAGCCCCGTCGCGGTGCCCTAGGCATATACCATAAGGCAACCGGGTCGGGCGCGAGGTAACCTCCCAGt                                                      >  2:833676/1‑140 (MQ=2)
                                                                                           aGGAGCGC‑GCAGGGGGCGGCCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTAACATCCCTGTAAAAcgc                                               <  2:8796130/140‑1 (MQ=32)
                                                                                                cgc‑gcAGGGGGCGGCCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTAACATCCCTGTAAAACGCGCCCt                                          <  2:1942375/140‑1 (MQ=32)
                                                                                                      aGGGGGCGGCCACTCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTAACATCCCTGTAAAACGCGCCCTCAGcc                                     <  2:7283641/140‑1 (MQ=17)
                                                                                                      aGGGGGCGGCCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTAACATCCCTGTAAAACGCGCCCTCAGcc                                     <  1:1857187/140‑1 (MQ=32)
                                                                                                       gggggCGGCCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTAACATCCCTGTAAAACGCGCCCTCAGccc                                    <  1:7504288/140‑1 (MQ=32)
                                                                                                       gggggCGGCCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTAACATCCCTGTAAAACGCGCCCTCAGccc                                    <  1:7504153/140‑1 (MQ=32)
                                                                                                          ggcggcCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTAACATCCCTGTAAAACGCGCCCTCAGCCcaca                                 <  2:2613603/140‑1 (MQ=32)
                                                                                                             ggcCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTAACATCCCTGTAAAACGCGCCCTCAGcc                                     <  1:8918208/133‑1 (MQ=32)
                                                                                                               ccAATCGCCTCCGCCCCCTGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTAACATCCCTGTAAAACGCGCCCTCAGCCCACATCCAt                            >  2:230535/1‑140 (MQ=17)
                                                                                                               ccAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTAACATCCCTGTAAAAcg                                                >  1:8147329/1‑120 (MQ=32)
                                                                                                               ccAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTAACATCCCTGTAAAACGCGCCCTCAGCCCACATCCAt                            <  2:1145176/140‑1 (MQ=32)
                                                                                                                  aTCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTAACATCCCTGTAAAACGCGCCCTCAGCCCACATCCAtgtg                         >  1:1310290/1‑140 (MQ=32)
                                                                                                                   tCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTAACATCCCTGTAGAACGCGCCCTCAGCCCACATCCATGTgg                        >  1:6245698/1‑140 (MQ=17)
                                                                                                                    cgccgccgcCCCCTGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTAACATCCCTGTAAAACGCGCCCTCAGCCCACATCCATGTggg                       >  2:6472204/1‑140 (MQ=32)
                                                                                                                    cgccgccgcCCCCTGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTAACATCCCTGTAAAACGCGCCCTCAGCCCACATCCATGTggg                       <  1:4833410/140‑1 (MQ=32)
                                                                                                                    cgccgccgcCCCCTGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTAACATCCCTGTAAAACGCGCCCTCAGCCCACATCCATGTggg                       <  2:6696401/140‑1 (MQ=32)
                                                                                                                    cgccgccgcCCCCTGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTAACATCCCTGTAAAACGCGCCCTCAGCCCACATCCATGTggg                       >  1:3544718/1‑140 (MQ=32)
                                                                                                                    cgccgccgcCCCCTGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTGATCCCGTACGGCAACCGGGTCGGGCGCGCGGTCACCTCCCTGTACCACGCGCCCTCCGCCCCCATCCATGTggg                       >  1:465643/1‑140 (MQ=11)
                                                                                                                      ccgccgccCCCTGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTAACATCCCTGTAAAACGCGCCCTCAGCCCACATCCATGTGGGCt                     >  1:6777922/1‑140 (MQ=32)
                                                                                                                         ccgccCCCTGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTAACATCCCTGTAAAACGCGCCCTCAGCCCACATCCATGTGGGCTGcc                  >  2:9368447/1‑140 (MQ=32)
                                                                                                                         ccgccCCCTGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTAACATCCCTGTAAAACGCGCCCTCAGCCCACATCCATGTGGGCTGcc                  >  2:5413751/1‑140 (MQ=32)
                                                                                                                          cgccCCCTGCACCCCCGGTCTCCGGCGCACACAATCGCCGCTGCGCCGTGCCCTCGGCTTATCCCTTACGGCTCCCGGGTCGGGCGCGAGGTAACATCCCTGTAAAACGCGCCCTCAGCCCACATCCATGTGGGCTGccc                 <  2:7007615/140‑1 (MQ=11)
                                                                                                                           gccCCCTGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTAACATCCCTGTAAAACGCGCCCTCAGCCCACATCCATGTGGGCTGcccc                <  1:238284/140‑1 (MQ=32)
                                                                                                                            cccccTGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTAACATCCCTGTAAAACGCGCCCTCAGCCCACATCCATGTGGGCTGCCCCg               <  1:5376245/140‑1 (MQ=32)
                                                                                                                                 tGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTAACATCCCTGTAAAACGCGCCCTCAGCCCACATCCATGTGGGCTGCCCCGGCCtt          >  1:1225775/1‑140 (MQ=32)
                                                                                                                                 tGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTAACATCCCTGTAAAACGCGCCCTCAGCCCACATCCATGTGGGCTGCCCCGGCCtt          >  2:4808818/1‑140 (MQ=32)
                                                                                                                                       cccGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTAACATCCCTGTAAAACGCGCCCTCAGCCCACATCCATGTGGGCTGCCCCGGCCTTCAGGGa    >  2:6161852/1‑140 (MQ=32)
                                                                                                                                       cccGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGGGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTCACATCCCTGTAAAACGCGCCCTCAGCCCACATCCATGTGGGCTGCCCCGGCCTTCAGGGa    >  2:4590699/1‑140 (MQ=17)
                                                                                                                                        ccGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTACCATCCCTGTAAAACGCGCCCTCAGCCCACATCCATGTGGGCTGCCCCGGCCTTCAGGGaa   >  2:6040813/1‑140 (MQ=17)
                                                                                                                                        ccGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTAACATCCCTGTAAAACGCGCCCTCAGCCCACATCCATGTGGGCTGcccc                >  1:5676983/1‑127 (MQ=32)
                                                                                                                                        ccGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTAACATCCCTGTAAAACGCGCCCTCAGCCCACATCCATGTGGGCTGcccc                <  2:5676983/127‑1 (MQ=32)
                                                                                                                                        ccGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTAACATCCCTGTAAAACGCGCCCTCAGCCCACATCCATGTGGGCTGCCCCGGCCTTCAGGGac   >  2:1860144/1‑139 (MQ=17)
                                                                                                                                        ccGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTAACATCCCTGTAAAACGCGCCCTCAGCCCACATCCATGTGGGCTGCCCCGGCCTTCAGGGaa   >  1:6457633/1‑140 (MQ=32)
                                                                                                                                        ccGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTAACATCCCTGTAAAACGCGCCCTCAGCCCACATCCATGTGGGCTGCCCCGGCCTTCAGGGaa   >  1:9259770/1‑140 (MQ=32)
                                                                                                                                         cGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTAACATCCCTGTAAAACGCGCCCTCAGCCCACATCCATGTGGGCTGCCCCGGCCTTCAGGGacc  >  2:9043106/1‑138 (MQ=17)
                                                                                                                                         cGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTAACATCCCTGTAAAACGCGCCCTCAGCCCACATCCATGTGGGCTGCCCCGGCCTTCAGGGAAc  >  1:3064981/1‑140 (MQ=32)
                                                                                                                                         cGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTAACATCCCTGTAAAACGCGCCCTCAGCCCACATCCATGTGGGCTGCCCCGGCCTTCAGGGAAc  >  1:7062619/1‑140 (MQ=32)
                                                                                                                                         cGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTAACATCCCTGTAAAACGCGCCCGCAGCCCACATCCATGTGGGCTGCCCCGGCCTTCAGGGAAc  >  1:4151968/1‑140 (MQ=17)
                                                                                                                                          |                                                                                                                                          
TGTCATCAGGTCCGAAAATAACGAGAATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGC‑GCAGGGGGCGGCCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTAACATCCCTGTAAAACGCGCCCTCAGCCCACATCCATGTGGGCTGCCCCGGCCTTCAGGGAAC  >  NZ_CP025268/2233111‑2233386

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑