Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0252683,505,5590TA26.0% 500.2 / 70.7 270intergenic (+90/+62)phoB/yhfSDNA‑binding transcriptional regulator FrlR/aminotransferase class I/II‑fold pyridoxal phosphate‑dependent enzyme
Reads supporting (aligned to +/- strand):  ref base T (55/133);  new base A (65/1);  total (135/135)
Fisher's exact test for biased strand distribution p-value = 9.62e-26
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

Only 100 of 287 total aligned reads displayed.

TCTGCCGCGCCAATCGCATCACCTTAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTC‑TCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAAAATCCGCAGCACCGTCTCTTCACCGCTGCGATTCGGGTTAATGCGAATCGC  >  NZ_CP025268/3505420‑3505698
                                                                                                                                           |                                                                                                                                            
tctGCCGCGCCAATCGCATCACCTTAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCct                                                                                                                                              <  1:7081578/140‑1 (MQ=255)
   gCCGCGCCAATCGCATCACCTTAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCACACCCGAACCCtc‑tc                                                                                                                                          >  2:702020/1‑140 (MQ=255)
         ccAATCGCATCACCTTAACTGTTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTGCCCACACCCAAAACCAC‑ACCCCaaa                                                                                                                                    >  1:3263942/1‑140 (MQ=38)
         ccAATCGCATCACCTTAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTGCCCGCACCCCAACCCCC‑TACCCcaa                                                                                                                                    >  1:2672965/1‑140 (MQ=38)
                cATCACCTTAACTATTGATAATAACAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTC‑TCCCCAAAGGGGCGa                                                                                                                             <  2:4153752/140‑1 (MQ=255)
                cATCACCTTAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTC‑TCCCCAAAGGGGCGa                                                                                                                             <  1:3519356/140‑1 (MQ=255)
                  tCACCTTAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTC‑TCCCCAAAGGGGCGAgg                                                                                                                           <  2:6294221/140‑1 (MQ=255)
                   cACCTTACCTATTGATAAGAACAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCGACCCCTC‑TCCCCACAGGGGCGAggg                                                                                                                          <  2:1657847/140‑1 (MQ=38)
                     ccTTAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTC‑TCCCCAAAGGGGCGAGGGGa                                                                                                                        <  1:3759997/140‑1 (MQ=255)
                     ccTTAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGGTCCCCTCACCCTAACCCTC‑TCCCCAAAGGGGCGAGGGGa                                                                                                                        <  1:5408922/140‑1 (MQ=255)
                        tAACTATTGATCATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGGTCCCCCCCCCCACACCCGC‑TCCCCAACGGGGCGAGGGGACCg                                                                                                                     >  2:7168794/1‑140 (MQ=38)
                        tAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTC‑TCCCCAAAGGGGCGAGGGGACCg                                                                                                                     >  2:1822146/1‑140 (MQ=255)
                          aCTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTC‑TCCCCAAAGGGGCGAGGGGACCGtt                                                                                                                   <  1:4756439/140‑1 (MQ=255)
                          aCTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCGAACCCTC‑TCCCCAACGGGGCGAGGGGACCGtt                                                                                                                   <  1:7766121/140‑1 (MQ=255)
                          aCTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCACACCCAAACCCAC‑TCCCCCAAGGGGCGAGGGGAcccgt                                                                                                                   >  2:219236/1‑137 (MQ=39)
                           cTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTACCCACACCCAAAAACAC‑TCCCCAAAAGGGCGAGGGGCCCGGTc                                                                                                                  >  1:6792309/1‑140 (MQ=37)
                            tATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTC‑TCCCCAAAGGGGCGAGGGGACCGTTCa                                                                                                                 <  1:6443397/140‑1 (MQ=255)
                             aTTGATAATAAATGACATTAACCGTAGGCCGGATACGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGGTCCCCTCACCCGAACCCTC‑TCCCCAAAGGGGCGAGGGGACCGTTCAg                                                                                                                >  2:5086950/1‑140 (MQ=255)
                             aTTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTACCCACCCCCAAACCCACAACCCCAAAGGGCCAGGGGGCCCGtaat                                                                                                                 >  1:3673036/1‑137 (MQ=25)
                              ttGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTAACCCAAACCCAC‑AACCCAAATGGGGGAGGGGTCCGtggagt                                                                                                               >  1:894717/1‑135 (MQ=37)
                              ttGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCACACCCAAACCCAC‑AACCCCAAAGGGCGGGGGGGCCCTGCAgg                                                                                                               >  1:2530308/1‑139 (MQ=37)
                               tGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTC‑TCCCCAAAGGGGCGAGGGGACCGTTCAGTa                                                                                                              <  1:3022265/140‑1 (MQ=255)
                               tGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTC‑TCCCCAAAGGGGCGAGGGGACCGTTCAGTa                                                                                                              <  1:8658361/140‑1 (MQ=255)
                               tGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCAAAACCAC‑TCCCCCAAGGGGCGCGGGGCCCGGTCAGTa                                                                                                              >  2:3428983/1‑140 (MQ=38)
                               tGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCACACCCAAACCCAC‑ACCCCCAAGGGGCGAGGGGACCCTTCAGTa                                                                                                              >  2:3262873/1‑140 (MQ=38)
                               tGATAATAAAAGACATTAACCGAAGGCCGGAGAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATACCGTACGTTACCCTCACGCAAACCCTC‑TCCCCCAAGGGGCGAGGGGACCGGTCCGTa                                                                                                              >  2:6569270/1‑140 (MQ=38)
                                gATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTGCCCTCACCCAAACCCAC‑ACACCAAAGGGGCGCGGGGACCGGTCCGTAc                                                                                                             >  1:8203127/1‑140 (MQ=38)
                                gATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTC‑TCCCCa                                                                                                                                      >  1:5536878/1‑115 (MQ=255)
                                gATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTC‑TCCCCCAAGGGGGGAGGGGACCGGTCAGTaa                                                                                                             >  2:3923202/1‑139 (MQ=255)
                                gATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCAC‑TCCCCAAAGGGGCGAGGGGACCGTTCAGTAc                                                                                                             >  2:3778754/1‑140 (MQ=255)
                                gATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCAAACCCAC‑TACCCAAAGGGGCGAGGGGACCGTTCAGTAc                                                                                                             >  1:6530086/1‑140 (MQ=255)
                                gATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCAACCAAAACCAC‑AACCCAAAGGGGCGCTGGGTCCGTTCataac                                                                                                             >  1:95984/1‑136 (MQ=37)
                                gATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCGCACCCTAACCCAA‑AACCCAAATGGGCGTGGGGAACGTTCAGTAc                                                                                                             >  1:6037863/1‑140 (MQ=38)
                                gATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCACAACCTAACCCAC‑TACCCAAAGGGGCGAGGGGACCGTTCAGAAc                                                                                                             >  2:5324819/1‑140 (MQ=39)
                                gATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTACCCACACCCAAACACAC‑TCCCCCAAGGGGCGAGGGGACCGTTCAGTAc                                                                                                             >  2:2728030/1‑140 (MQ=38)
                                cataatcAAAGACATTAACCGTAGGCAGGATAAGATGCGCCAGCATCTCATCCGGCGATGCTGGCGCGTTGAATTTTACATACCGTACGTTACCCCAACCCTAACCCAC‑AGCCCAAAGGGGCGATGGGACCGTTCCGtga                                                                                                             >  2:2468342/2‑138 (MQ=37)
                                     taaAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCACACCCAAAACCAC‑AACCCAAAGGGGGGAGGGGACCGTGCCGTAAGGAAt                                                                                                        >  1:6485371/1‑140 (MQ=37)
                                            cATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGGTCCCCTCACCCTAACCCTC‑TCCCCACAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCtt                                                                                                 <  2:9257274/140‑1 (MQ=255)
                                             aTTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCCCCCCCCAACCCAC‑AACCCCAAAGGGCGCGGGGGCCCGGCAGTAAGGAAattttaatt                                                                                                >  1:3199786/1‑134 (MQ=21)
                                             aTTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCACACCCTAAACCAC‑TCCCCAAATGGGCGAGGGGACCGTTCAGTACGGAAATTATCTTg                                                                                                >  2:56844/1‑140 (MQ=38)
                                              ttAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTC‑TCCCCACAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGa                                                                                               <  1:3981511/140‑1 (MQ=255)
                                              ttAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTC‑TCCCCACAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGa                                                                                               <  2:2394027/140‑1 (MQ=255)
                                              ttAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTC‑TCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGa                                                                                               <  2:6920584/140‑1 (MQ=255)
                                              ttAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTGACATCCCGTACGTTCCCCTCCCCCTAACCCTC‑TCCCCAACGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGa                                                                                               <  2:4172995/140‑1 (MQ=255)
                                              tgaaCCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTC‑TCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGa                                                                                               <  2:7494206/138‑1 (MQ=255)
                                               tAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCACACCCAAACCCAC‑ACCCCCCAGGGGCGGGGGGTCCCGGCAGTACGGGAATTAGCATggc                                                                                              >  2:2077266/1‑138 (MQ=25)
                                               tAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTACCCACACCCAAACCCAC‑AACCCAAAAGGGAGAGGGGACCGATAAGTACCGAAATTAACATGAc                                                                                              >  2:8029562/1‑140 (MQ=25)
                                                 aCCGTNGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCACACCCTAACCCAC‑TCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGTCgg                                                                                            >  1:7626198/1‑140 (MQ=255)
                                                   cGTCGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTC‑TCCCCCAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTg                                                                                          <  2:6751990/140‑1 (MQ=255)
                                                   cGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTC‑TCCCCCCAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTg                                                                                          <  1:105813/140‑1 (MQ=255)
                                                       ggCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTGCCCAAACCCAAACCCCC‑GCCCCCAAGGGGGGCGGGGGCCGGTCCGTACCGAATTTAGCTTGACGGGGcgag                                                                                      >  1:1581162/1‑137 (MQ=25)
                                                       ggCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTC‑TCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTAt                                                                                      >  2:8748389/1‑140 (MQ=255)
                                                        gCCGGCAAAGCTGCGCCAGCCTCGCATCCGGCGATGCTTGCGCGAAGAATTTTACATACCGTACCTACCCCTCAACCTAACCCTC‑TCCCCAAAGGGGCGAGGGGACCGTACATTACGGAATTTAGCCTGACGGTGCTATc                                                                                     <  2:3983758/140‑1 (MQ=25)
                                                        gCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTC‑TCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATc                                                                                     <  1:1643184/140‑1 (MQ=255)
                                                        gCCGCATAAGATGCGCCAGCCTCGCATCGGGCGATGCTGGCGCGTTGAATTTTACCTCCCGTACGTTCCCCTCACCCTAACCCAC‑TCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATc                                                                                     <  1:8368703/140‑1 (MQ=38)
                                                          cGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTC‑TCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCa                                                                                   <  1:2123340/140‑1 (MQ=255)
                                                           ggATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTACCCGACCCCCAAAACAC‑ACACCCAAGGGGCGAGGGGTACGGTCACACCGGAATATAGCCTGACGGTGcgaacccc                                                                                  >  1:8768476/1‑133 (MQ=255)
                                                            gATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTNCGTTCCCCACACCCAAACCCAC‑ACCCCCAAGGGGCGGGGGGGACGGTAAGTAAGGGATTTATAGTTAAGGTGCTATcaacc                                                                                 >  1:6283819/1‑136 (MQ=255)
                                                            gATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCACACCCTAACCCTC‑TCCCCAAAGGGGCGAGGGGACCGGTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGc                                                                                 >  2:7373090/1‑140 (MQ=255)
                                                            gATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCACACCCAAAACCAC‑ACCCCAAAGGGGCGAGGGGACCGGTCAGTAAGGAAATTAGCTTGACGGTGCTATACAGc                                                                                 >  2:2556740/1‑140 (MQ=255)
                                                             aTAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGGTCCCCGGGCCAGGACCCCC‑ACCCCAAAGGGGGGAGGGGTCCGGTAAGTAAGGAAATTATGTTGTCGGTGCTAtaccgcg                                                                                >  1:393457/1‑134 (MQ=255)
                                                                aGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTC‑TCCCCCAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTa                                                                             <  1:5033188/140‑1 (MQ=255)
                                                                    gcgcCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTACCCCTC‑TCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATa                                                                         <  1:1182119/140‑1 (MQ=255)
                                                                      gcCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTC‑TCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACt                                                                       <  2:4813889/140‑1 (MQ=255)
                                                                       ccAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTGCCCACACCCAAACCCGC‑AACCCCAAGGGGCGGGGGGGCCCGTCAGTACGGAAATTAGGTTTTCGGGGGTGTACCGCAGTAAATaaag                                                                      >  1:9141977/1‑137 (MQ=255)
                                                                       ccAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCAAACCCAC‑ACCCCAAAGGGGCGAGGGGACCGTTCAGTAAGGAAATTAGATTGACGGTGCTATACAGCaataaataaag                                                                      >  2:4739048/1‑137 (MQ=37)
                                                                             tcgcatcCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTC‑TCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATa                                                                <  1:5513808/140‑1 (MQ=255)
                                                                             tcgcatcCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTC‑TCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATa                                                                <  1:4356466/140‑1 (MQ=255)
                                                                               gcatcCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTC‑TCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATAct                                                              <  1:5748787/140‑1 (MQ=255)
                                                                                         aTGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTC‑TCCCCCAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCaaa                                                    <  2:8876995/140‑1 (MQ=255)
                                                                                         aTGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTC‑TCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCaaa                                                    <  1:4493569/140‑1 (MQ=255)
                                                                                         aTGCTGGCGCGTTGAAATTTACATCCCGTACGTTCCCCTCACCCTAACCCTC‑TCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCaaa                                                    <  2:4788535/140‑1 (MQ=255)
                                                                                                cgcgTTGAATTTTACATCCCGTACGTTCCCCGCACCCTAAACCAC‑TACCCCAAGGGGCGAGGGGGCCGGTCAGTACGGAATTTAGCGTGACGGTGCTATACAGCAATAAATAAAGGCCAAACTCACACGCAAAATCCgcc                                             >  2:5800402/1‑139 (MQ=255)
                                                                                                    ttGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTC‑TCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAAAATCCGCAGCAc                                         <  2:5503770/140‑1 (MQ=255)
                                                                                                    atGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTC‑TCACCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAAAATCCGCAGCAc                                         <  2:4589696/139‑1 (MQ=255)
                                                                                                     tGAATTTTACATCCCGTACGTTCCCCTCACCCTACCCCTC‑TCCCCAAAGGGGCGAGGGGACCGGTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAAAATCCGCAGCAcc                                        <  2:2793467/140‑1 (MQ=255)
                                                                                                     tGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTC‑TCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAAAATCCGCAGCAcc                                        <  1:1019082/140‑1 (MQ=255)
                                                                                                         ttttACATCCCGTACGTTCCCCTCACCCTAACCCTC‑TCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAAAATCCGCAGCACCGtct                                    <  2:9101/140‑1 (MQ=255)
                                                                                                               aTCCCGTACGGTCCCCTCACCCTAACCCTC‑TCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAAAATCCGCAGCACCGTCTCTTCAc                              <  1:1485469/140‑1 (MQ=255)
                                                                                                                   cGTACGTTACCCACACCCTAACCCAC‑TACCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCATGACGGTGCTATCCAGCAATAAAAACTGGCAAAACTCTCACGCAAAATCCGCAGCACCGTCACTTCAcccca                          >  2:5103164/1‑137 (MQ=255)
                                                                                                                     tACGTTCCCCACAACCTAACCCAC‑TCCCCAAAGGGGCGATGGGACCGTTCAGTAAGGAATTTAACTTTACGGTGCTATCCAGCATTAAATAAAGGCAATACTCTCAAGCAAAATCAGCAGCACCCTCTCTTCAACGCttc                        >  2:8462865/1‑138 (MQ=255)
                                                                                                                      aCGTTCCCCACACCCAAACCCAC‑TCCCCAAAGGGGCGAGGGGACCGGTCAGTACCGAAATTATCATTACGGTGCTAAACAGCATTAAATACTGGCAAAAAACTAACGCAAAAAACGCAGCACCCTCTCATCACCGCTGCg                       >  2:1844383/1‑140 (MQ=255)
                                                                                                                         ttCCCCTCACCCTAACCCTC‑TCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAAAATCCGCAGCACCGTCTCTTCACCGCTGCGAtt                    <  1:1895997/140‑1 (MQ=255)
                                                                                                                           ccccTCACCCTAACCCTC‑ACCCCAAAGGGGCGAGGGGAACGTTCAGTACGGAATTTAGCATGACGGTGCTATCCAGCATTAAATACTGGCAATAATCTCACGCAAAATCCGCCGCACCGTCTCTTCACCGCTGCGATTCg                  >  2:7765566/1‑140 (MQ=38)
                                                                                                                             ccTCAACCTAACCCTC‑TCCCCAAAGGGGCGAGGGGACCGGTCAGTACGGAATGTAGCTTGACGGTGCTAACCAGCATTAAATACTGGCACTACTCTCACGCAAAATCCGCAGCACCGTCTCTACACCGCTGCGATTCggg                >  2:1898953/1‑140 (MQ=39)
                                                                                                                              cTCACCCTAACCCTC‑TCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAAAATCCGCAGCACCGTCTCTTCACCGCTGCGATTCGGGt               <  2:5971446/140‑1 (MQ=255)
                                                                                                                                  cccAAACCCGC‑ACCCCAAAGGGGCGACGGGACCGTTAAGTACGGCAATTAGCTTGACGGTGCGAACCAGCATTAAAAAATGGCAAAACACTAACGCAAAATCCGCAGCACCCACACGACACCCCGGCGAATAGGGTTAAt           >  2:6195801/1‑140 (MQ=255)
                                                                                                                                      aaCCCTC‑TCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAAAATCCGCAGCACCGTCTCTTCACCGCTGCGATTCGGGTTAATGCGa       >  1:1739517/1‑140 (MQ=255)
                                                                                                                                      aaCCCTC‑TCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAAAATCCGCAGCACCGTCTCTTCACCGCTGCGATTCGGGTTAATGCGa       >  1:7253160/1‑140 (MQ=255)
                                                                                                                                      aaCCCTC‑TCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAAAATCCGCAGCACCGTCTCTTCACCGCTGCGATTCGGGTTAATGCGa       >  1:5801291/1‑140 (MQ=255)
                                                                                                                                      aaCCCTC‑TCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAAAATCCGCAGCACCGTCTCTTCACCGCTGCGATTCGGGTTAATGCGa       >  1:1996975/1‑140 (MQ=255)
                                                                                                                                      aaCCCTC‑TCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAAAATCCGCAGCACCGTCTCTTCACCGCTGCGATTCGGGTTAATGCGa       >  2:296071/1‑140 (MQ=255)
                                                                                                                                      aaCCCTC‑TCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAAAATCCGCAGCACCGACTCTTCACCGCTGCGATTCGGGTTAATGCGa       >  1:8494751/1‑140 (MQ=255)
                                                                                                                                      aaCCCTC‑TCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAAAATCCGCAGAACCTTCTCTTCACCGCTGCGATTCGGGTTAATGCGa       >  2:8638314/1‑140 (MQ=255)
                                                                                                                                       aCCCTC‑TCCCCACAGGGGCGAGGGGACCGTTCAGTACGGAATTGAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCGCACGCAAAATCCGCAGCACCGTCTCGTCACCGCTGCGATTCGGGTTAATGCGac      <  2:5040804/140‑2 (MQ=255)
                                                                                                                                         ccTC‑TCCCCACAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAAAATCCGCAGCACCGTCTCTTCACCGCTGCGATTCGGGTTAATGCGAATc    <  1:2246966/140‑1 (MQ=255)
                                                                                                                                           tc‑tcCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAAAATCCGCAGCACCGTCTCTTCACCGCTGCGATTCGGGTTAATGCGAATCGc  >  1:1436277/1‑140 (MQ=255)
                                                                                                                                           tc‑tcCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAAAATCCGCAGCACCGTCTCTTCACCGCTGCGATTCGGGTTAATGCGAATCGc  >  1:9120910/1‑140 (MQ=255)
                                                                                                                                           tc‑tcCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAAAATCCGCAGCACCGTCTCTTCACCGCTGCGATTCGGGTTAATGCGAATCGc  >  1:4100304/1‑140 (MQ=255)
                                                                                                                                           tc‑tcCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAAAATCCGCAGCACCGTCTCTTCACCGCTGCGATTCGGGTTAATGCGAATCGc  >  1:9356555/1‑140 (MQ=255)
                                                                                                                                           |                                                                                                                                            
TCTGCCGCGCCAATCGCATCACCTTAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTC‑TCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAAAATCCGCAGCACCGTCTCTTCACCGCTGCGATTCGGGTTAATGCGAATCGC  >  NZ_CP025268/3505420‑3505698

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑