BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP025268	543,804	T→C	H180R (CAT→CGT)	allC ←	allantoate deiminase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP025268	543,804	0	T	C	99.6%	1672.1 / NA	480	H180R (CAT→CGT)	allC	allantoate deiminase
Reads supporting (aligned to +/- strand): ref base T (0/0); major base C (305/173); minor base A (1/1); total (306/174)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 489 total aligned reads displayed.

GGTTTGATTCGCCGTTCAGCGTTACCGTATAACGACGCTGCCCGACAATTGCATTCACCACGCCAATTGATTGCCCATTACTTTCCAGCACACAGCCCTGTTCAATATGCAGTTCAACAAAGGCTTTAATATCCTGATGCGGAGTTAGTGGGGCGTTCGGAAGAGTAAATCCGCAAGCCTTCATCGCATCGACAAAACTATTTCCTTTGGCATCACAGATATTCCGCACGTCGTCAGGATTCGCCAGCCCAAAAATATTTTTACTGCCCCAGAA  >  NZ_CP025268/543667‑543940

ggTTTGATTCGCCGTTCAGCGTTACCGTATAACGACGCTGCCCGACAATTGCATTCACCACGCCAATTGATTGCCCATTACTTTCCAGCACACAGCCCTGTTCAATATGCAGTTCAACAAAGGCTTTAATATCCTGACGc                                                                                                                                        <  2:6938409/140‑1 (MQ=255)

 gTTTGATTCGCCGTTCAGCGTTACCGTATAACGACGCTGCCCGACAATTGCATTCACCACGCCAATTGATTGCCCATTACTTTCCAGCACACAGCCCTGTTCAATATGCAGTTCAACAAAGGCTTTAATATCCTGAcgcg                                                                                                                                       >  2:5560324/1‑140 (MQ=255)

 gTTTGATTCGCCGTTCAGCGTTACCGTATAACGACGCTGCCCGACAATTGCATTCACCACGCCAATTGATTGCCCATTACTTTCCAGCACACAGCCCTGTTCAATATGCAGTTCAACAAAGGCTTTAATATCCTGAcgcg                                                                                                                                       >  2:4297310/1‑140 (MQ=255)

 gTTTGATTCGCCGTTCAGCGTTACCGTATAACGACGCTGCCCGACAATTGCATTCACCACGCCAATTGATTGCCCATTACTTTCCAGCACACAGCCCTGTTCAATATGCAGTTCAACAAAGGCTTTAATATCCTGAcgcg                                                                                                                                       >  1:4703729/1‑140 (MQ=255)

 gTTTGATTCGCCGTTCAGCGTTACCGTATAACGACGCTGCCCGACAATTGCATTCACCACGCCAATTGATTGCCCATTACTTTCCAGCACACAGCCCTGTTCAATATGCAGGTCAACAAAGGCTTTAATATCCTGAcgcg                                                                                                                                       >  2:9251355/1‑140 (MQ=255)

  tttGAGTCGCCGTTCAGCGTTCCCGGAGAACGTCGCTGCCCGACACTTGCATTCACCACGCCAACTGATGGCCCTGTACTTGCCAGCCCCCAGCCCTGTTGTAGATGCAGTTCACCAAAGGCTTTAATATCCTGACGCgg                                                                                                                                      <  2:6675456/140‑1 (MQ=255)

   ttGATTCGCCGTTCAGCGTTACCGTATAACGACGCTGCCCGACAATTGCATTCACCACGCCAATTGATTGCCCATTACTTTCCAGCACACAGCCCTGTTCAATATGCAGTTCAACAAAGGCTTTAATATCCTGACGCGGa                                                                                                                                     <  2:3139080/140‑1 (MQ=255)

       ttCGCCGTTCAGCGTTACCGTATAACGACGCTGCCCGACAATTGCATTCACCACGCCAATTGATTGCCCATTACTTTCCAGCACACAGCCCTGTTCAATATGCAGTTCAACAAAGGCTTTAATATCCTGACGCGGAGTTa                                                                                                                                 >  2:81419/1‑140 (MQ=255)

                cAGCGTTACCGTATAACGACGCTGCCCGACAATTGCATTCACCACGCCAATTGATTGCCCATTACTTTCCAGCACACAGCCCTGTTCAATATGCAGTTCAACAAAGGCTTTAATATCCTGACGCGGAGTTAGTGGGGCGt                                                                                                                        >  1:2543444/1‑140 (MQ=255)

                    gtcaCCGTATAACGACGCTGCCCGACAATTGCATTCACCACGCCAATTGATTGCCCATTACTTTCCAGCACACAGCCCTGTTCAATATGCAGTTCAACAAAGGCTTTAATATCCTGACGCGGAGTTAGTGGGGCGTTCgg                                                                                                                    <  1:7731340/137‑1 (MQ=255)

                    gTTACCGTATAACGACGCTGCCCGACAATTGCATTCACCACGCCAATTGATTGCCCATTACTTTCCAGCACACAGCCCTGTTCAATATGCAGTTCAACAAAGGCTTTAATATCCTGACGCGGAGTTAGTGGGGCGTTCgg                                                                                                                    <  1:657994/140‑1 (MQ=255)

                    gTTACCGTATAACGACGCTGCCCGACAATTGCATTCACCACGCCAATTGATTGCCCATTACTTTCCAGCACACAGCCCTGTTCAATATGCAGTTCAACAAAGGCTTTAATATCCTGACGCGGAGTTAGTGGGGCGTTCgg                                                                                                                    <  2:53818/140‑1 (MQ=255)

                           tataACGACGCTGCCCGACAATTGCATTCACCACGCCAATTGATTGCCCATTACTTTCCAGCACACAGCCCTGTTCAATATGCAGTTCAACAAAGGCTTTAATATCCTGACGCGGAGTTAGTGGGGCGTTCGGAAGAGTa                                                                                                             >  1:7429787/1‑140 (MQ=255)

                             taACGACGCTGCCCGACAATTGCATTCACCACGCCAATTGATTGCCCATTACTTTCCAGCACACAGCCCTGTTCAATATGCAGTTCAACAAAGGCTTTAATATCCTGACGCGGAGTTAGTGGGGCGTTCGGAAGAGTaaa                                                                                                           >  1:789342/1‑140 (MQ=255)

                                   cgCTGCCCGACAATTGCATTCACCACGCCAATTGATTGCCCATTACTTTCCAGCACACAGCCCTGTTCAATATGCAGTTCAACAAAGGCTTTAATATCCTGACGCGGAGTTAGTGGGGCGTTCGGAAGAGTAAATCCGCa                                                                                                     <  2:1801120/140‑1 (MQ=255)

                                   cgCTGCCCGACAATTGCATTCACCACGCCAATTGATTGCCCATTACTTTCCAGCACACAGCCCTGTTCAATATGCAGTTCAACAAAGGCTTTAATATCCTGACGCGGAGTTAGTGGGGCGTTCGGAAGAGTAAATCCGCa                                                                                                     <  1:8587825/140‑1 (MQ=255)

                                   cgCTGCCCGACAATTGCATTCACCACGCCAATTGATTGCCCATTACTTTCCAGCACACAGCCCTGTTCAATATGCAGTTCAACAAAGGCTTTAATATCCTGACGCGGAGTTAGTGGGGCGTTCGGAAGAGTAAATCCGCa                                                                                                     >  2:2749129/1‑140 (MQ=255)

                                   cgCTGCCCGACAATTGCATTCACCACGCCAATTGATTGCCCATTACTTTCCAGCACACAGCCCTGTTCAATATGCAGTTCAACAAAGGCTTTAATATCCTGACGCGGAGTTAGTGGGGCGTTCGGAAGAGTAAATCCGCa                                                                                                     >  2:5060581/1‑140 (MQ=255)

                                     cTGCCCGACAATTGCATTCACCACGCCAATTGATTGCCCATTACTTTCCAGCACACAGCCCTGTTCAATATGCAGTTCAACAAAGGCTTTAATATCCTGACGCGGAGTTAGTGGGGCGTTCGGAAGAGTAAATCCGCAAg                                                                                                   >  2:2164201/1‑140 (MQ=255)

                                     cTGCCCGACAATTGCATTCACCACGCCAATTGATTGCCCATTACTTTCCAGCACACAGCCCTGTTCAATATGCAGTTCAACAAAGGCTTTAATATCCTGACGCGGAGTTAGTGGGGCGTTCGGAAGAGTAAATCCGCAAg                                                                                                   >  2:4914552/1‑140 (MQ=255)

                                                ttGCATTCACCACGCCAATTGATTGCCCATTACTTTCCAGCACACAGCCCTGTTCAATATGCAGTTCAACAAAGGCTTTAATATCCTGACGCGGAGTTAGTGGGGCGTTCGGAAGAGTAAATCCGCAAGCCTTcatcgca                                                                                        >  1:9016818/1‑140 (MQ=255)

                                                     ttCACCACGCCAATTGATTGCCCATTACTTTCCAGCACACAGCCCTGTTCAATATGCAGTTCAACAAAGGCTTTAATATCCTGACGCGGAGTTAGTGGGGCGTTCGGAAGAGTAAATCCGCAAGCCTTCATCGCATCGAc                                                                                   <  1:8271157/140‑1 (MQ=255)

                                                     ttCACCACGCCAATTGATTGCCCATTACTTTCCAGCACACAGCCCTGTTCAATATGCAGTTCAACAAAGGCTTTAATATCCTGACGCGGAGTTAGTGGGGCGTTCGGAAGAGTAAATCCGCAAGCCTTCATCGCATCGAc                                                                                   >  2:5658930/1‑140 (MQ=255)

                                                      tCACCACGCCAATTGATTGCCCATTACTTTCCAGCACACAGCCCTGTTCAATATGCAGTTCAACAAAGGCTTTAATATCCTGACGCGGAGTTAGTGGGGCGTTCGGAAGAGTAAATCCGCAAGCCTTCATCGCATCGACa                                                                                  <  1:92168/140‑1 (MQ=255)

                                                        accacGCCAATTGATTGCCCATTACTTTCCAGCACACAGCCCTGTTCAATCTGCAGTTCAACAAAGGCTTTAATATCCTGACGCGGAGTTAGTGGGGCGTTCGGAAGAGTACATCCGCAAGCCTTCATCGCATCGACaaa                                                                                >  2:7164352/1‑140 (MQ=255)

                                                        accacGCCAATTGATTGCCCATTACTTTCCAGCACACAGCCCTGTTCAATATGCAGTTCAACAAAGGCTTTAATATCCTGACGCGGAGTTAGTGGGGCGTTCGGAAGAGTAAATCCGCAAGCCTTCATCGCATCGACaaa                                                                                >  1:1514493/1‑140 (MQ=255)

                                                        accacGCCAATTGATTGCCCATTACTTTCCAGCACACAGCACTGTTCAATATGCAGTTCAACAAAGGCTTTAATATCCTGACGCGGAGTTAGTGGGGCGTTCGGAAGAGTAAATCCGCAAGCCTTCATCGCATCGACaaa                                                                                >  2:1728880/1‑140 (MQ=255)

                                                            cGCCAATTGATTGCCCATTACTTTCCAGCACACAGCCCTGTTCAATATGCAGTTCAACAAAGGCTTTAATATCCTGACGCGGAGTTAGTGGGGCGTTCGGAAGAGTAAATCCGCAAGCCTTCATCGCATCGACAAAACTa                                                                            >  2:4559594/1‑140 (MQ=255)

                                                             gCCAATTGATTGCCCATTACTTTCCAGCACACAGCCCTGTTCAATATGCAGTTCAACAAAGGCTTTAATATCCTGACGCGGAGTTAGTGGGGCGTTCGGAAGAGTAAATCCGCAAGCCTTcatcgcat                                                                                       >  1:4865814/1‑128 (MQ=255)

                                                             gCCAATTGATTGCCCATTACTTTCCAGCACACAGCCCTGTTCAATATGCAGTTCAACAAAGGCTTTAATATCCTGACGCGGAGTTAGTGGGGCGTTCGGAAGAGTAAATCCGCAAGCCTTCATCGCATCGACAAAACTAt                                                                           >  1:1354315/1‑140 (MQ=255)

                                                             gCCAATTGATTGCCCATTACTTTCCAGCACACAGCCCTGTTCAATATGCAGTTCAACAAAGGCTTTAATATCCTGACGCGGAGTTAGTGGGGCGTTCGGAAGAGTAAATCCGCAAGCCTTCATCGCATCGACAAAACTAt                                                                           <  2:3049543/140‑1 (MQ=255)

                                                                 attgattgCCCATTACTTTCCAGCACACAGCCCTGTTCAATATGCAGTTCAACAAATGGTTTAATATCCTGACGCGGAGTTAGTGGGGCGTTCGGAAGAGTAAATCCGCAAGCCTTCATCGCATCGACAAAACTATTTcc                                                                       <  2:801976/140‑1 (MQ=255)

                                                                   tgattgCCCATGACTTTCCAGCACACAGCCCTGTTCAATATGCAGTTCAACAAAGGCTTTAATATCCTGACGCGGAGTTAGTGGGGCGTTCGGAAGAGTAAATCCGCAAGCCTTCATCGCATCGACAAAACTATTTCCtt                                                                     <  2:6395042/140‑1 (MQ=255)

                                                                     attgCCCATTACTTTCCAGCACACAGCCCTGTTCAATATGCAGTTCAACAAAGGCTTTAATATCCTGACGCGGAGTTAGTGGGGCGTTCGGAAGAGTAAATCCGCAAGCCTTCATCGCATCGACAAAACTATTTCCTTTg                                                                   <  2:4754882/140‑1 (MQ=255)

                                                                       tgCCCATTACTTTCCAGCACACAGCCCTGTTCAATATGCAGTTCAACAAAGGCTTTAATATCCTGACGCGGAGTTAGTGGGGCGTTCGGAAGAGTAAATCCGCAAGCCTTCATCGCATCGACAAAACTATTTCCTTTGGc                                                                 <  1:1611496/140‑1 (MQ=255)

                                                                         cccATTACTTTCCAGCACACAGCCCTGTTCAATATGCAGGTCAACAAAGGCTTTAATATCCTGACGCGGAGTTAGTGGGGCGTTCGGAAGAGTAAATCCGCAAGCCTTCATCGCATCGACTATACTATTTCCTTTGGCAt                                                               >  1:5019382/1‑140 (MQ=255)

                                                                              tACTTTCCAGCACACAGCCCTGTTCAATATGCAGTTCAACAAAGGCTTTAATATCCTGACGCGGAGTTAGTGGGGCGTTCGGAAGAGTAAATCCGCAAGCCTTCATCGCATCGACAAAACTATTTCCTTTGGCATCACAg                                                          <  2:2275147/140‑1 (MQ=255)

                                                                                    ccAGCACACAGCCCTGTTCAATATGCAGTTCAACAAAGGCTTTAATATCCTGACGCGGAGTTAGTGGGGCGTTCGGAAGAGTAAATCCGCAAGCCTTCATCGCATCGACAAAACTATTTCCTTTGGCATCACAGATATTc                                                    >  1:7827071/1‑140 (MQ=255)

                                                                                       gCACACAGCCCTGTTCAATATGCAGTTCAACAAAGGCTTTAATATCCTGACGCGGAGTTAGTGGGGCGTTCGGAAGAGTACATCCGCAAGCCTTCATCGCATCGACAAAACTATTTCCTTTGGCATCACAGATATTCCGc                                                 >  2:7106726/1‑140 (MQ=255)

                                                                                        cacacaGCCCTGTTCAATATGCAGTTCAACAAAGGCTTTAATATCCTGACGCGGAGTTAGTGGGGCGTTCGGAAGAGTAAATCCGCAAGCCTTCATCGCATCGACAAAACTATTTCCTTTGGCATCACAGATATTCCGCa                                                >  1:2200570/1‑140 (MQ=255)

                                                                                        cacacaGCCCTGTTCAATATGCAGTTCAACAAAGGCTTTAATATCCTGACGCGGAGTTAGTGGGGCGTTCGGAAGAGTAAATCCGCAAGCCTTCATCGCATCGACAAAACTATTTCCTTTGGCATCACAGATATTCCGCa                                                <  1:2305261/140‑1 (MQ=255)

                                                                                        cacacaGCACTGTTCAATATGCAGTTCAACAAAGGCTTTAATATCCTGACGCGGAGTTAGTGGGGCGTTCGGAAGAGTAAATCCGCTAGCCTTCATCGCATCGACAATACTATTTCCTTTGGCATCACAGATATTCCGCa                                                <  2:3952227/140‑1 (MQ=255)

                                                                                         acacaGCCCTGTTCAATATGCAGTTCAACAAAGGCTTTAAGATCCTGACGCGGAGTTAGTGGGGCGTTCGGAAGAGTAAATCCGCAAGCCTTCATCGCATCGACAAAACTATTTCCTTTGGCATCACAGATATTCCGCAc                                               <  2:7353662/140‑1 (MQ=255)

                                                                                          cacaGCCCTGTTCAATATGCAGTTCAACAAAGGCTTTAATATCCTGACGCGGAGTTAGTGGGGCGTTCGGAAGAGTAAATCCGCAAGCCTTCATCGCATCGACAAAACTATTTCCTTTGGCATCACAGATATTCCGCAcg                                              <  1:6089462/140‑1 (MQ=255)

                                                                                             aGCCCTGTTCAATATGCAGTTCAACAAAGGCTTTAATATCCTGACGCGGAGTTAGTGGGGCGTTCGGAAGAGTAAATCCGCAAGCCTTCATCGCATCGACAAAACTATTTCCTTTGGCATCACAGATATTCCGCAcgtcg                                           >  2:3382884/1‑140 (MQ=255)

                                                                                             aGCCCTGTTCAATATGCAGTTCAACAAAGGCTTTAATATCCTGACGCGGAGTTAGTGGGGCGTTCGGAAGAGTAAATCCGCAAGCCTTCATCGCATCGACAAAACTATTTCCTTTGGCATCACAGATATTCCGCAcgtcg                                           >  2:8772116/1‑140 (MQ=255)

                                                                                             aGCCCTGTTCAATATGCAGTTCAACAAAGGCTTTAATATCCTGACGCGGAGTTAGTGGGGCGTTCGGAAGAGTAAATCCGCAAGCCTTCATCGCATCGACAAAACTATTTCCTTTGGCATCACAGATATTCCGCAcgtcg                                           >  2:2891042/1‑140 (MQ=255)

                                                                                             aGCCCTGTTCAATATGCAGTTCAACAAAGGCTTTAATATCCTGACGCGGAGTTAGTGGGGCGTTCGGAAGAGTAAATCCGCAAGCCTTCATCGCATCGACAAAACTATTTCCTTTGGCATCACAGATATTCCGCAcgtcg                                           >  1:3218397/1‑140 (MQ=255)

                                                                                             aGCCCTGTTCAATATGCAGTTCAACAAAGGCTTTAATATCCTGACGCGGAGTTAGTGGGGCGTTCGGAAGAGTAAATCCGCAAGCCTTCATCGCATCGACAAAACTATTTCCTTTGGCATCACAGATATTCCGCAcgtcg                                           >  1:6399463/1‑140 (MQ=255)

                                                                                             aGCCCTGTTCAATATGAAGTTCAACAAAGGCTTTAATATCATGACGCGGAGTTAGTGGGGCGTTCGGAAGAGTAAATCCGCAAGCCTTCATCGCATCGACAAAACTATTTCCTTTGGCATCACAGATATTCCGCAcgtcg                                           >  2:3810702/1‑140 (MQ=255)

                                                                                             aGCCCTGTTCAATAGGCAGTTCAACAAAGGCTTTAATATCCTGTCGCGGAGGTCGTGGGGCGTTCGGAAGAGTAAATCCGCAAGCCTTCATCGCATCGACATAACTATGTCCTTTGGCATCTCAGATATTCCGCGcgtcg                                           >  2:3009517/1‑140 (MQ=255)

                                                                                              gCCCTGTTCACTATTCAGTTCAACAAAGGCTTTAATATCCTGAAGCGGAGTTAGTGGGGCGTTCGGACGAGTAAATCCGCAAGCCTTCATCGCATCGACAAAACTATTTCCTTTGGCATCACAGATATTCCGCAcgtcgt                                          <  2:7733745/140‑1 (MQ=255)

                                                                                               cccTGTTCAATATGCAGTTCAACAAAGGCTTTAATATCCTGACGCGGAGTTAGTGGGGCGTTCGGAAGAGTAAATCCGCAAGCCTTCATCGCATCGACAAAACTATTTCCTTTGGCATCACAGATATTCCGCACgtcgtc                                         >  1:2433357/1‑140 (MQ=255)

                                                                                               cccTGTTCAATATGCAGTTCAACAAAGGCTTTAATATCCTGACGCGGAGTTAGTGGGGCGTTCGGAAGAGTAAATCCGCAAGCCTTCATCGCATCGACAAAACTATTTCCTTTGGCATCACAGATATTCCGCACgtcgtc                                         >  1:950034/1‑140 (MQ=255)

                                                                                               cccTGTTCAATATGCAGTTCAACAAAGGCTTTAATATCCTGACGCGGAGTTAGTGGGGCGTTCGGAAGAGTAAATCCGCAAGCCTTCATCGCATCGACAAAACTATTTCCTTTGGCATCACAGATATTCCGCACgtcgtc                                         >  1:4588905/1‑140 (MQ=255)

                                                                                               cccTGTTCAATATGCAGTTCAACAAAGGCTTTAATATCCTGACGCGGAGTTAGTGGGGCGTTCGGAAGAGTAAATCCGCAAGCCTTCATCGCATCGACAAAACTATTTCCTTTGGCATCACAGATATTCCGCACgtcgtc                                         >  2:3581939/1‑140 (MQ=255)

                                                                                               cccTGTTCAATATGCAGTTCAACAAAGGCTTTAATATCCTGACGCGGAGTTAGTGGGGCGTTCGGAAGAGTAAATCCGCAAGCCTTCATCGCATCGACAAAACTATTTCCTTTGGCATCACAGATATTCCGCACgtcgtc                                         >  1:5885665/1‑140 (MQ=255)

                                                                                                  tGTTCAATATGCAGTTCAACAAAGGCTTTAATATCCTGACGCGGAGTTAGTGGGGCGTTCGGAAGAGTAAATCCGCAAGCCTTCATCGCATCGACAAAACTATTTCCTTTGGCATCACAGATATTCCGCACGTCGTCAgg                                      >  1:2063482/1‑140 (MQ=255)

                                                                                                  tGTTCAATATGCAGTTCAACAAAGGCTTTAATATCCTGACGCGGAGTTAGTGGGGCGTTCGGAAGAGTAAATCCGCAAGCCTTCATCGCATCGACAAAACTATTTCCTTTGGCATCACAGATATTCCGCACGTCGTCAgg                                      >  1:168314/1‑140 (MQ=255)

                                                                                                    ttCAATATGCAGTTCAACAAAGTCTTTAATATCCTGAAGCGGATTTACTGGCGCGTTAGGAAGTGTAAATCCTCAAGCATTCATCGAATCTACAAAACTATTTACTTTGGCATCACAGATATTACGCTCGTCGTCAGTAt                                    >  2:4392959/1‑140 (MQ=255)

                                                                                                    ttCAATATGCAGTTCAACAAAGGCTTTAATATCCTGACGCGGAGTTAGTGGGGCGTTCGGAAGAGTAAATCCGCAAGCCTTCATCGCATCGACAAAACTATTTCCTTTGGCATCACAGATATTCCGCACGTCGTCAGGAt                                    >  1:8912793/1‑140 (MQ=255)

                                                                                                         tatGCAGTTCAACAAAGGCTTTAATATCCTGACGCGGAGTTAGTGGGGCGTTCGGAAGAGTAAATCCGCAAGCCTTCATCGCATCGACAAAACTATTTCCTTTGGCATCACAGATATTCCGCACGTCGTCAGGATTCGcc                               >  1:1006601/1‑140 (MQ=255)

                                                                                                         tatGCAGTTCAACAAAGGCTTTAATATCCTGACGCGGAGTTAGTGGGGCGTTCGGAAGAGTAAATCCGCAAGCCTTCATCGCATCGACAAAACTATTTCCTTTGGCATCACAGATATTCCGCACGTCGTCAGGATTCGcc                               >  1:8749755/1‑140 (MQ=255)

                                                                                                         tatGCAGTTCAACAAAGGCTTTAATATCCTGACGCGGAGTTAGTGGGGCGTTCGGAAGAGTAAATCCGCAAGCCTTCATCGCATCGACAAAACTATTTCCTTTGGCATCACAGATATTCCGCACGTCGTCAGGATTCGcc                               >  1:8380046/1‑140 (MQ=255)

                                                                                                         tatGAAGTTCAACAAAGGCTATAATATCCTGACGCGGAGTTAGAGGTGCGTTCGGAAGAGTAAATCCGCAAGCCTACATCGCATCGACAAAACTATTTCCATTGCAATCACAGATATTCAGCACGTCGAAATGATTCTcc                               >  2:6259080/1‑140 (MQ=255)

                                                                                                              aGTTCAACAAAGGCTTTAATATCCTGACGCGGAGTTAGTGGGGCGTTCGGAAGAGTAAATCCGCAAGCCTTCATCGCATCGACAAAACTATTTCCTTTGGCATCACAGATATTCCGCACGTCGTCAGGATTCGCCAGccc                          >  2:6800106/1‑140 (MQ=255)

                                                                                                              aGTTCAACAAAGGCTTTAATATCCTGACGCGGAGTTAGTGGGGCGTTCGGAAGAGTAAATCCGCAAGCCTTCATCGCATCGACAAAACTATTTCCTTTGGCATCACAGATATTCCGCACGTCGTCAGGATTCGCCAGccc                          >  1:5278265/1‑140 (MQ=255)

                                                                                                              aGTTCAACAAAGGCTTTAATATCCTGACGCGGAGTTAGTGGGGCGTTCGGAAGAGTAAATCCGCAAGCCTTCATCGCATCGACAAAACTATTTCCTTTGGCATCACAGATATTCCGCACGTCGTCAGGATTCGCCAGccc                          >  1:8133349/1‑140 (MQ=255)

                                                                                                              aGTTCAACAAAGGCTTTAATATCCTGACGCGGAGTTAGTGGGGCGTTCGGAAGAGTAAATCCGCAAGCCTTCATCGCATCGACAAAACTATTTCCTTTGGCATCACAGATATTCCGCACGTCGTCAGGATTCGCCAGccc                          >  1:6892382/1‑140 (MQ=255)

                                                                                                              aGTTCAACAAAAGCTTTAATATCCTGACGCGGAGTTAGTGGGGCGTTCGGAAGAGTAAATCCGCAAGCCTTCATCGCATCGACAAAACTATTTCCGTTGGCATCACTGATCTTCCGCACGTCGTCAGGATTCGCCAGccc                          >  2:8246142/1‑140 (MQ=255)

                                                                                                               gTTCAACAAAGGCTTTAATATCCTGACGCGGAGTTAGTGGGGCGTTCGGAAGAGTAAATCCGCAAGCCTTCATCGCATCGACAAATCTATTTCCTTTGGCATCACAGATATTCCGCACGTCGTCAGGATTCGCCAGCCCa                         >  1:7590442/1‑140 (MQ=255)

                                                                                                               gTTCAACAAAGGCTTTAATATCCTGACGCGGAGTTAGTGGGGCGTTCGGAAGAGTAAATCCGCAAGCCTTCATCGCATCGACAAAACTATTTCCTTTGGCATCACAGATATTCCGCACGTCGTCAGGATTCGCCAGCCCa                         >  1:4143709/1‑140 (MQ=255)

                                                                                                                ttCAACAAAGGCTTTAATATCCTGACGCGGAGTTAGTGGGGCGTTCGGAAGAGTAAATCCGCAAGCCTTCATCGCATCGACAAAACTATTTCCTTTGGCATCACAGATATTCCGCACGTCGTCAGGATTCGCCAGCCCaa                        >  1:4062588/1‑140 (MQ=255)

                                                                                                                ttCAACAAAGGCTTTAATATCCTGACGCGGAGTTAGTGGGGCGTTCGGAAGAGTAAATCCGCAAGCCTTCATCGCATCGACAAAACTATTTCCTTTGGCATCACAGATATTCCGCACGTCGTCAGGATTCGCCAGCCCaa                        >  1:5470708/1‑140 (MQ=255)

                                                                                                                ttCAACAAAGGCTGTCATCTCCTGACGCGGAGTTAGTGGGGCGTTCGGAAGAGTAAATCCGCTAGCCGTCATCGCATCGACAAAACTATTTCCTTTGGCATCACAGAGATTCCGCACGGCGGCAGGATTCGCCCGCCCaa                        >  2:1960463/1‑140 (MQ=255)

                                                                                                                 tCAACAAAGGCTTTAATATCCTGACGCGGAGTTAGTGGGGCGTTCGGAAGAGTAAATCCGCAAGCCTTCATCGCATCGACAAAACTATTTCCTTTGGCATCACAGATATTCCGCACGTCGTCAGGATTCGCCAGCCCaaa                       <  1:2599365/140‑1 (MQ=255)

                                                                                                                 tCAACAAAGGCTTTAATATCCTGACGCGGAGTTAGTGGGGCGTTCGGAAGAGTAAATCCGCAAGCCTTCATCGCATCGACAAAACTATTTCCTTTGGCATCACAGATATTCCGCACGTCGTCAGGATTCGCCAGCCCaaa                       <  2:8545067/140‑1 (MQ=255)

                                                                                                                 tCAACAAAGGCTTTAATATCCTGACGCGGAGTTAGTGGGGCGTTCGGAAGAGTAAATCCGCAAGCCTTCATCGCATCGACAAAACTATTTCCTTTGGCATCACAGATATTCCGCACGTCGTCAGGATTCGCCAGCCCaaa                       >  1:5628540/1‑140 (MQ=255)

                                                                                                                   aacaaAGGCTTTAATATCCTGACGCGGAGTTAGTGGGGCGTTCGGAAGAGTAAATCCGCAAGCCTTCATCGCATCGACAAAACTATTTCCTTTGGCATCACAGATATTCCGCACGTCGTCAGGATTCGCCAGCCCaaaaa                     >  2:127605/1‑140 (MQ=255)

                                                                                                                        aGGCTTTAATATCCTGACGCGGAGTTAGTGGGGCGTTCGGAAGAGTAAATCCGCAAGCCTTCATCGCATCGACAAAACTATTTCCTTTGGCATCACAGATATTCCGCACGTCGTCAGGATTCGCCAGCCCAAAAATAttt                >  2:135450/1‑140 (MQ=255)

                                                                                                                          gCTTTAATATCCTGACGCGGAGTTAGTGGGGCGTTCGGAAGAGTAAATCCGCAAGCCTTCATCGCATCGACAAAACTATTTCCTTTGGCATCACAGATATTCCGCACGTCGTCAGGATTCGCCAGCCCAAAAATAttttt              >  1:673751/1‑140 (MQ=255)

                                                                                                                          gCTTTAATATCCTGACGCGGAGTTAGTGGGGCGTTCGGAAGAGTAAATCCGCAAGCCTTCATCGCATCGACAAAACTATTTCCTTTGGCATCACAGATATTCCGCACGTCGTCAGGATTCGCCAGCCCAAAAATAttttt              >  1:709630/1‑140 (MQ=255)

                                                                                                                          gCTTTAATATCCTGACGCGGAGTTAGTGGGGCGTTCGGAAGAGTAAATCCGCAAGCCGTCATCGCAGCGACAAAACTATTTCCGTTGGCATCACAGATTTTCAGCACGTCGTCAGGATTCGCCAGCCCGAAAGTAttgtt              >  2:4225135/1‑140 (MQ=255)

                                                                                                                            tttAATATCCTGACGCGGAGTTAGTGGGGCGTTCTGAAGAGTAAATCCGATAGCCTTCATCGCATCGACAAAACTATTTCCTTTTGCATCACAGATATTCCGCACGTCGTCAGGATTCGCCAGCCCAAAAATATTTTTAc            <  2:1534397/140‑1 (MQ=255)

                                                                                                                            tttAATATCCTGACGCGGAGTTAGTGGGGCGTTCGGCAGAGTAAATCCGCAAGCCTTCATCGCATCGACAAAACTATTTCCTTTGGCAACACAGATATTCCGCACGTCGTCAGGATTCGCCAGCCCAAAAATATTTTTAc            <  2:1440567/140‑1 (MQ=255)

                                                                                                                            tttAATATCCTGACGCGGAGTTAGTGGGGCGTTCGGAAGAGTAAATCCGCAAGCCTTCATCGCATCGACAAAACTATTTCCTTTGGCATCACAGATATTCCGCACGTCGTCAGGATTCGCCAGCCCAAAAATATTTTTAc            <  1:3312409/140‑1 (MQ=255)

                                                                                                                            tttAATATCCTGACGCGGAGTTAGTGGGGAGTTCGGAAGAGTAAATCCGCAATCCTTAATCGCATCGACAAAACTATTTCCTTTGGCATCACAGATATTCAGCACTTCGTCAGGATTCGCCAGCCCAAAAATATTTTTAc            <  2:2640264/140‑1 (MQ=255)

                                                                                                                             ttAATATCCTGACGCGGAGTTAGTGGGGCGTTCGGAAGAGTAAATCCGCAAGCCTTCATCGCATCGACAAAACTATTTCCTTTGGCATCACAGATATTCCGCACGTCGTCAGGATTCGCCAGCCCAAAAATATTTTTACt           >  1:3659723/1‑140 (MQ=255)

                                                                                                                              tAATATCCTGACGCGGAGTTAGTGGGGCGTTCGGAAGAGTAAATCCGCAAGCCTTCATCGCATCGACAAAACTATTTCCTTTGGCATCACAGATATTCCGCACGTCGTCAGGATTCGCCAGCCCAAAAATATTTTTACTg          <  1:4308134/140‑1 (MQ=255)

                                                                                                                              tAATATCCTGACGCGGAGTTAGTGGGGCGTTCGGAAGAGTAAATCCGCAAGCCTTCATCGCATCGACAAAACTATTTCCTTTGGCATCACAGATATTCCGCACGTCGTCAGGATTCGCCAGCCCAAAAATATTTTTACTg          >  1:3015827/1‑140 (MQ=255)

                                                                                                                              tAATATCCTGACGCGGAGTTAGTGGGGCGTTCGGAAGAGTAAATCCGCAAGCCTTCATCGCATCGACAAAACTATTTCCTTTGGCATCACAGATATTCCGCACGTCGTCAGGATTCGCCAGCCCAAAAATATTTTTACTg          >  1:4229816/1‑140 (MQ=255)

                                                                                                                              tAATATCCTGACGCGGAGTTAGTGGGGCGTTCGGAAGAGTAAATCCGCAAGCCTTCATCGCATCGACAAAACTATTTCCTTTGGCATCACAGATATTCCGCACGTCGTCAGGATTCGCCAGCCCAAAAATATTTTTACTg          <  1:8222184/140‑1 (MQ=255)

                                                                                                                                atatCCTGACGCGGAGTTAGTGGGGCGTTCGGAAGAGTAAATCCGCAAGCCTTCATCGCATCGACAAAACTATTTCCTTTGGCATCACAGATATTCCGCACGTCGTCAGGATTCGCCAGCCCAAAAATATTTTTACTGcc        <  2:1096947/140‑1 (MQ=255)

                                                                                                                                    ccTGACGCGGAGTTAGTGGGGCGTTCGGAAGAGTAAATCCGCAAGCCTTCATCGCATCGACAAAACTATTTCCTTTGGCAt                                                               <  2:7584414/81‑1 (MQ=255)

                                                                                                                                    ccTGACGCGGAGTTAGTGGGGCGTTCGGAAGAGTAAATCCGCAAGCCTTCATCGCATCGACAAAACTATTTCCTTTGGCATCACAGATATTCCGCACGTCGTCAGGATTCGCCAGCCCAAAAATATTTTTACTGCCCCag    <  1:3870377/140‑1 (MQ=255)

                                                                                                                                    ccTGACGCGGAGTTAGTGGGGCGTTCGGAAGAGTAAATCCGCAAGCCTTCATCGCATCGACAAAACTATTTCCTTTGGCATCACAGATATTCCGCACGTCGTCAGGATTCGCCAGCCCAAAAATATTTTTACTGCCCCag    >  2:9127417/1‑140 (MQ=255)

                                                                                                                                    ccTGACGCGGAGTTAGTGGGGCGTTCGGAAGAGTAAATCCGCAAGCCTTCATCGCATCGACAAAACTATTTCCTTTGGCATCACAGATATTCCGCACGTCGTCAGGATTCGCCAGCCCAAAAATATTTTTACTGCCCCag    >  2:9360309/1‑140 (MQ=255)

                                                                                                                                    ccTGACGCGGAGTTAGTGGGGCGTTCGGAAGAGTAAATCCGCAAGCCTTCATCGCATCGACAAAACTATTTCCTTTGGCATCACAGATATTCCGCACGTCGTCAGGATTCGCCAGACCAAAAATATTTTTACTGCCCCag    <  2:6070714/140‑1 (MQ=255)

                                                                                                                                     cTGACGCGGAGTTAGTGGGGCGTTCGGAAGAGTAAATCCGCAAGCCTTCATCGCATCGACAAAACTATTTCCTTTGGCATCACAGATATTCCGCACGTCGTCAGGATTCGCCAGCCCAAAAATATTTTTACTGCCCCaga   <  2:8049998/140‑1 (MQ=255)

                                                                                                                                      tGACGCGGAGTTTGTGGGGCGTTCGGAAGAGTAAATCCGCAAGCCTTCATCGCATCGACAAAACTATTTCCTTTGGCATCACAGATATTCCGCACGTCGTCAGGATTCGCCAGCCCAAAAATATTTTTACTGCCCCagaa  <  2:7941854/140‑1 (MQ=255)

GGTTTGATTCGCCGTTCAGCGTTACCGTATAACGACGCTGCCCGACAATTGCATTCACCACGCCAATTGATTGCCCATTACTTTCCAGCACACAGCCCTGTTCAATATGCAGTTCAACAAAGGCTTTAATATCCTGATGCGGAGTTAGTGGGGCGTTCGGAAGAGTAAATCCGCAAGCCTTCATCGCATCGACAAAACTATTTCCTTTGGCATCACAGATATTCCGCACGTCGTCAGGATTCGCCAGCCCAAAAATATTTTTACTGCCCCAGAA  >  NZ_CP025268/543667‑543940

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`