BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP025268	581,201	T→C	pseudogene (667/667 nt)	CXP41_RS03085 ←	class I SAM‑dependent methyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP025268	581,201	0	T	C	99.6%	2521.4 / NA	729	pseudogene (667/667 nt)	CXP41_RS03085	class I SAM‑dependent methyltransferase
Reads supporting (aligned to +/- strand): ref base T (0/0); major base C (405/321); minor base A (0/3); total (405/324)
Fisher's exact test for biased strand distribution p-value = 8.73e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 729 total aligned reads displayed.

ATATCGGGTGTTGCTGAACCGTGTTGATACATCAACTGCACCTGATATTGAGTGGCCTACGAACCCTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGCGGT‑T‑G‑ATGTTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTAACATTCTCGAAATGCTCTGAGAGTATATGTGTTAAGCCTTCTTCGGAATCTTTTGTGTTTGAAAAGATGCCTTTCTGATTGT  >  NZ_CP025268/581069‑581337

           tGCTGAACCGTGTTGATACATCAACTGCACCTGATATTGAGTGGCCTACGAACCCTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTAAAGCAAGAACAAGCTGCGGTGG‑G‑ATGCAATTTTGTACCTGAAGc                                                                                                                         >  1:7026342/1‑140 (MQ=38)

               gAACCGTGTTGATACATCAACTGCACCTGATATTGAGTGGCCTACGAACCCTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGCGGT‑TGG‑ATGCTATTTTTTTCCTGAAGCGGaa                                                                                                                     <  2:8319140/140‑1 (MQ=255)

               gAACCGTGTTGATACATCAACTGCACCTGATATTGAGTGGCCTACGAACCCTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGCGGT‑TGG‑ATGCTATTTTTTTCCTGAAGCGGaa                                                                                                                     <  1:2516979/140‑1 (MQ=255)

               gAACCGTGTTGATACATCAACTGCACCTGATATTGAGTGGCCTACGAACCCTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGCGGT‑TGG‑ATGCTATTTTTTTCCTGAAGCGGaa                                                                                                                     <  1:9003194/140‑1 (MQ=255)

               gAACCGTGTTGATACATCAACTGCACCTGATATTGAGTGGCCTACGAACCCTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGCGGT‑TGG‑ATGCTATTTTTTTCCTGAAGCGGaa                                                                                                                     <  1:2741040/140‑1 (MQ=255)

                 aacGTGTTGATAAATCAACTGCACCTTATATTGAGTGGCCTACGAACCCTGTCAGTTAGTAATCATTGGGATTATGCCGCAGAAAGTCTTAAGCTAGAACATGATGAGGT‑T‑GTATGATATTTTTTTCATGAAGCGGtaaa                                                                                                                   <  2:2835291/138‑1 (MQ=255)

                 aCCTTGTTGATACATCATCTGCACCTGATATTGAGTGGCCTACGAACCCTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGCGGT‑TGG‑ATGCTATTTTTTTCCTGAAGCGGaaaa                                                                                                                   <  2:8668098/140‑1 (MQ=255)

                  ccGTGTTGATACATCAACTGCACCTGATATTGAGTGGCCTACGAACCCTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGCGGT‑TGG‑ATGCTATTTTTTTCCTGAAGCGGAAAAc                                                                                                                  >  2:1379064/1‑140 (MQ=255)

                    gtgtTGATACATCAACTGCACCTGATATTGAGTGGCCTACGAACCCTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGCGGT‑TGG‑ATGCTATTTTTTTCCTGAAGCGGAAAACAt                                                                                                                <  1:7657910/140‑1 (MQ=255)

                    gtgtTGATACATCAACTGCACCTGATATTGAGTGGCCTACGAACCCTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGCGGT‑TGG‑ATGCTATTTTTTTCCTGAAGCGGAAAACAt                                                                                                                <  2:2041382/140‑1 (MQ=255)

                    gtgtTGATACATCAACTGCACCTGATATTGAGTGGCCTACGAACCCTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGCGGT‑TGG‑ATGCTATTTTTTTCCTGAAGCGGAAAACAt                                                                                                                >  2:2284446/1‑140 (MQ=255)

                    gtgtTGATACATCAACTGCACCTGATATTGAGTGGCCTACGAACCCTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGCGGT‑TGG‑ATGCTATTTTTTTCCTGAAGCGGAAAACAt                                                                                                                <  1:2971051/140‑1 (MQ=255)

                     tgtTGATACATCAACTGCACCTGATATTGAGTGGCCTACGAACCCTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGCGGT‑TGG‑ATGCTATTTTTTTCCTGAAGCGGaaaa                                                                                                                   <  2:872771/136‑1 (MQ=255)

                     tgtTGATACATCAACTGCACCTGATATTGAGTGGCCTACGAACCCTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGCGGT‑TGG‑ATGCTATTTTTTTCCTGAAGCGGAAAACAtt                                                                                                               >  2:8448505/1‑140 (MQ=255)

                     tgtTGATACATCAACTGCACCTGATATTGAGTGGCCTACGAACCCTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGCGGT‑TGG‑ATGCTATTTTTTTCCTGAAGCGGAAAACAtt                                                                                                               >  2:3763040/1‑140 (MQ=255)

                     tgtTGATACATCAACTGCACCTGATATTGAGTGGCCTACGAACCCTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGCGGT‑TGG‑ATGCTATTTTTTTCCTGAAGCGGAAAACAtt                                                                                                               >  1:4603342/1‑140 (MQ=255)

                     tgtTGATACATCAACTGCACCTGATATTGAGTGGCCTACGAACCCTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGCGGT‑TGG‑ATGCTATTTTTTTCCTGAAGCGGAAAACAtt                                                                                                               >  1:4398364/1‑140 (MQ=255)

                     tgtTGATACATCAACTGCACCTGATATTGAGTGGCCTACGAACCCTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGCGGT‑TGG‑ATGCTATTTTTTTCCTGAAGCGGAAAACAt                                                                                                                >  1:6193914/1‑139 (MQ=255)

                      gtTGATACATCAACTGCACCTGATATTGAGTGGCCTACGAACCCTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGCGGT‑TGG‑ATGCTATTTTTTTCCTGAAGCGGAAAACATta                                                                                                              <  1:1289551/140‑1 (MQ=255)

                          aTACATCAACTGCACCTGATATTGAGTGGCCTACGAACCCTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGCGGT‑TGG‑ATGCTATTTTTTTCCTGAAGCGGAAAACATtactac                                                                                                          >  2:6589818/1‑140 (MQ=255)

                           tACATCAACTGCACCTGATATTGAGTGGCCTACGAACCCTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGCGGT‑TGG‑ATGCTATTTTTTTCCTGAAGCGGAAAACATTACTACa                                                                                                         <  2:9069030/140‑1 (MQ=255)

                               tCAACTGCACCTGATATTGAGTGGCCTACGAACCCTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGCGGT‑TGG‑ATGCTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTAc                                                                                                     >  2:3059340/1‑140 (MQ=255)

                               tCAACTGCACCTGATATTGAGTGGCCTACGAACCCTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGCGGT‑TGG‑ATGCTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTAc                                                                                                     >  2:5987043/1‑140 (MQ=255)

                               tCAACTGCACCTGATATTGAGTGGCCTACGAACCCTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGCGGT‑TGG‑ATGCTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTAc                                                                                                     >  1:7746338/1‑140 (MQ=255)

                               tCAACTGCACCTGATATTGAGTGGCCTACGAACCCTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGCGGT‑TGG‑ATGCTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTAc                                                                                                     >  1:6128827/1‑140 (MQ=255)

                                cAACTGCACCTGATATTGAGTGGCCTACGAACCCTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGCGGT‑TGG‑ATGCTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTAcc                                                                                                    <  1:5387432/140‑1 (MQ=255)

                                    tGCACCTGATATTGAGTGGCCTACGAACCCTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGCGGT‑TGG‑ATGCTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGa                                                                                                >  1:2626349/1‑140 (MQ=255)

                                     gCACCTGATATTGAGTGGCCTACGAACCCTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGCGGT‑TGG‑ATGCTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGaa                                                                                               >  2:473367/1‑140 (MQ=255)

                                      cACCTGATATTGAGTGGCCTACGAACCCTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGCGGT‑TGG‑ATGCTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAAc                                                                                              >  2:1145555/1‑140 (MQ=255)

                                      cACCTGATATTGAGTGGCCTACGAACCCTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGCGGT‑TGG‑ATGCTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAAc                                                                                              >  2:5692444/1‑140 (MQ=255)

                                       aCCTGATATTGAGTGGCCTACGAACCCTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGCGGT‑TGG‑ATTCTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAAcc                                                                                             <  2:353362/140‑1 (MQ=255)

                                       aCCTGATATTGAGTGGCCTACGAACCCTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGCGGT‑TGG‑ATGCTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAAcc                                                                                             <  1:4205040/140‑1 (MQ=255)

                                       aCCTGATATTGAGTGGCCTACGAACCCTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGCGGT‑TGG‑ATGCTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAAcc                                                                                             <  1:378263/140‑1 (MQ=255)

                                       aCCTGATATTGAGTGGCCTACGAACCCTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGCGGT‑TGG‑ATGCTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAAcc                                                                                             <  1:5249998/140‑1 (MQ=255)

                                        ccTGATGTTGAGTGGCCTACGAACCCTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGCGGT‑TGG‑ATGCTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCt                                                                                            <  1:1653226/140‑1 (MQ=255)

                                        ccTGATATTGAGTGGCCTACGAACCCTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGCGGT‑TGG‑ATGCTATTTTTTTCCTGAAGCGGaa                                                                                                                     >  1:9081556/1‑115 (MQ=255)

                                        ccTGATATTGAGTGGCCTACGAACCCTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGCGGT‑TGG‑ATGCTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCt                                                                                            >  1:6753490/1‑140 (MQ=255)

                                        ccTGATATTGAGTGGCCTACGAACCCTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGCGGT‑TGG‑ATGCTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCt                                                                                            >  1:5887898/1‑140 (MQ=255)

                                             tatTGAGTGGCCTACGAACCCTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGCGGT‑TGG‑ATGCTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGtt                                                                                       >  1:7481157/1‑140 (MQ=255)

                                              atTGAGTGGCCTACGAACCCTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGCGGT‑TGG‑ATGCTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGttt                                                                                      >  1:8182939/1‑140 (MQ=255)

                                                 gAGTGGCCTACGAACCCTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGCGGT‑TGG‑ATGCTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTaa                                                                                   <  1:3947845/140‑1 (MQ=255)

                                                  aGTGGCCTACGAACCCTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGCGGT‑TGG‑ATGCTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTAAc                                                                                  >  2:5892587/1‑140 (MQ=255)

                                                  aGTGGCCTACGAACCCTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGCGGT‑TGG‑ATGCTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTAAc                                                                                  >  2:5422537/1‑140 (MQ=255)

                                                  aGTGGCCTACGAACCCTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGCGGT‑TGG‑ATGCTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTAAc                                                                                  >  1:3365026/1‑140 (MQ=255)

                                                  aGTGGCCTACGAACCCTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGCGGT‑TGG‑ATGCTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTAAc                                                                                  >  1:8033982/1‑140 (MQ=255)

                                                   gTGGCCTACGAACCCTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGCGGT‑TGG‑ATGCTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTAACa                                                                                 >  1:5456099/1‑140 (MQ=255)

                                                      gCCTACGAACCCTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGCGGT‑TGG‑ATGCTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTAACATtc                                                                              >  2:7910419/1‑140 (MQ=255)

                                                      gCCTACGAACCCTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGCGGT‑TGG‑ATGCTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTAACATtc                                                                              >  1:4864755/1‑140 (MQ=255)

                                                       ccTACGAACCCTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGCGGT‑TGG‑ATGCTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTAACATtct                                                                             >  1:7393447/1‑140 (MQ=255)

                                                           cGAACCCTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGCGGT‑TGG‑ATGCTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTAACATTCTCGaa                                                                         <  1:5661921/140‑1 (MQ=255)

                                                           cGAACCCTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGCGGT‑TGG‑ATGCTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTAACATTCTCGaa                                                                         <  2:6964388/140‑1 (MQ=255)

                                                            gAACCCTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGCGGT‑TGG‑ATGCTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTAACATTCTCGaaa                                                                        <  2:2674354/140‑1 (MQ=255)

                                                              aCCCTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGCGGT‑TGG‑ATGCTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTAACATTCTCGAAATg                                                                      <  2:5012872/140‑1 (MQ=255)

                                                                ccTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGCGGT‑TGG‑ATGCTATTATTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTAACATTCTCGAAATGct                                                                    <  2:1718380/140‑1 (MQ=255)

                                                                 cTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGCGGT‑TGG‑ATGCTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTTGTTTTAACATTCTCGAAATGctc                                                                   <  2:4515724/140‑1 (MQ=255)

                                                                 cTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGCGGT‑TGG‑ATGCTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTAACCTTCTCGAAATGctc                                                                   >  2:5269469/1‑140 (MQ=255)

                                                                 cTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGCGGT‑TGG‑ATGCTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTAACATTCTCGAAATGctc                                                                   >  2:4113697/1‑140 (MQ=255)

                                                                 cTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGCGGT‑TGG‑ATGCTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTAACATTCTCGAAATGctc                                                                   >  1:2914115/1‑140 (MQ=255)

                                                                  tGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGCGGT‑TGG‑ATGCTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTAACATTCTCGAAATGctct                                                                  >  1:5151947/1‑140 (MQ=255)

                                                                  tGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGCGGT‑TGG‑ATGCTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTAACATTCTCGAAATGctct                                                                  <  1:1942623/140‑1 (MQ=255)

                                                                    tCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGCGGT‑TGG‑ATGCTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTAACATTCTCGAAATGCTCTga                                                                <  1:9415547/140‑1 (MQ=255)

                                                                    tCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGCGGT‑TGG‑ATGCTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTAACATTCTCGAAATGCTCTga                                                                <  2:1890534/140‑1 (MQ=255)

                                                                    tCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGCGGT‑TGG‑ATGCTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTAACATTCTCGAAATGCTCTga                                                                <  2:5628839/140‑1 (MQ=255)

                                                                    tCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGCGGT‑TGG‑ATGCTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTAACATTCTCGAAATGCTCTga                                                                <  2:7582045/140‑1 (MQ=255)

                                                                     cAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGCGGT‑TGG‑ATGCTATTTTTGTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTAACATTCTCGAAATGCTCTgag                                                               <  2:2434231/140‑1 (MQ=255)

                                                                          aGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGCGGT‑TGG‑ATGCTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTAACATTCTCGAAATGCTCTGAGAGtat                                                          >  2:8241963/1‑140 (MQ=255)

                                                                              aTCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGCGGT‑TGG‑ATGCTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTAACATTCTCGAAATGCTCTGAGAGTATAtgt                                                      >  1:4970530/1‑140 (MQ=255)

                                                                              aTCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGCGGT‑TGG‑ATGCTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTAACATTCTCGAAATGCTCTGAGAGTATAtgt                                                      >  2:6153425/1‑140 (MQ=255)

                                                                              aTCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGCGGT‑TGG‑ATGCTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTAACATTCTCGAAATGCTCTGAGAGTATAtgt                                                      >  1:7217193/1‑140 (MQ=255)

                                                                                   tGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGCGGT‑TGG‑ATGCTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTAACATTCTCGAAATGCTCTGAGAGTATATGTGTTaa                                                 >  1:8681469/1‑140 (MQ=255)

                                                                                   tGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGCGGT‑TGG‑ATGCTATTTTTTTCCTGAAGCGG‑AAACATTACTACAGTACCTTGAACCTTGGTTTTAACATTCTCGAAATGCTCTGAGAGTATATGTGTTAAg                                                >  1:2325941/1‑140 (MQ=255)

                                                                                      gATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGCGGT‑TGG‑ATGCTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTAACATTCTCGAAATGCTCTGAGAGTATATGTGTTAAGcc                                              >  1:1115164/1‑140 (MQ=255)

                                                                                       tttATGCCGCAGCTCGTCTTAAGCAAGAACATGCTGCGGT‑TGG‑ATTATTTTTTTTTCCTGAAGCGGTTAACATTACTACAGTACCTTTTACCTTGGTTTTAACATTCTCTAAATGCTATGAGAGTATTTGTGTTAAGCct                                             <  1:3192746/139‑1 (MQ=255)

                                                                                        ttATGCCGCAGCACGTCTTAAGCAAGAACATGCTGCGGT‑TGG‑ATGCTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTAACATTCTCGAAATGCTCTGAGAGTATATGTGTTAAGCctt                                            >  1:3469183/1‑140 (MQ=255)

                                                                                          aTGCCGCAGCACGTCTTAAGCAAGAACATGCTGCGGT‑TGG‑ATGCTATTTATTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTAACATTCTCGAAATGCTCTGAGAGTATATGTGTTAAGCcttct                                          >  2:3613583/1‑140 (MQ=255)

                                                                                           tGCCGCAGCACGTCTTAAGCAAGAACATGCTGCGGT‑TGG‑ATGCTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTAACATTCTCGAAATGCTCTGAGAGTATATGTGTTAATCcttatt                                         >  2:7312483/1‑140 (MQ=255)

                                                                                           tGCCGCAGCACGTCTTAAGCAAGAACATGCTGCGGT‑TGG‑ATGCTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTAACATTCTCGAAATGCTCTGAGAGTATATGTGTTAAGCcttctt                                         >  2:6086893/1‑140 (MQ=255)

                                                                                              cGCAGCACGTCTTAAGCAAGAACATGCTGCGGT‑TGG‑ATGCTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTAACATTCTCGAAATGCTCTGAGAGTATATGTGTTAAGCCTTCTTCgg                                      <  2:3293641/140‑1 (MQ=255)

                                                                                               gcagcaCGTCTTAAGCAAGAACATGCTGCGGT‑TGG‑ATGCTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTAACATTCTCGAAATGCTCTGAGAGTATATGTGTTAAGCCTTCTTCGGa                                     <  2:6493538/140‑1 (MQ=255)

                                                                                                cagcaCGTCTTAAGCAAGAACATGCTGCGGT‑TGG‑ATGCTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTAACATTCTCGAAATGCTCTGAGAGTATATGTGTTAAGCCTTCTTCGGaa                                    >  1:6848374/1‑140 (MQ=255)

                                                                                                   caCGTCTTAAGCAAGAACATGCTGCGGT‑TGG‑ATGCTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTAACATTCTCGAAATGCTCTGAGAGTATATGTGTTAAGCCTTCTTCGGAATCt                                 >  2:9375591/1‑140 (MQ=255)

                                                                                                     cGTCTTAAGCAAGAACATGCTGCGGT‑TGG‑ATGCTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTAACATTCTCGAAATGCTCTGAGAGTATATGTGTTAAGCCTTCTTCGGAATCttt                               >  2:9269421/1‑140 (MQ=255)

                                                                                                     cGTCTTAAGCAAGAACATGCTGCGGT‑TGG‑ATGCTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTAACATTCTCGAAATGCTCTGAGAGTATATGTGTTAAGCCTTCTTCGGAATCttt                               >  1:1499748/1‑140 (MQ=255)

                                                                                                     cGTCTTAAGCAAGAACATGCTGCGGT‑TGG‑ATGCTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTAACATTCTCGAAATGCTCTGAGAGTATATGTGTTAAGCCTTCTTCGGAATCttt                               >  1:1787922/1‑140 (MQ=255)

                                                                                                            aGCAAGAACATGCTGCGGT‑TGG‑ATGCTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTAACATTCTCGAAATGCTCTGAGAGTATATGTGTTAAGCCTTCTTCGGAATCTTTTGTGttt                        <  1:7841058/140‑1 (MQ=255)

                                                                                                            aGCAAGAACATGCTGCGGT‑TGG‑ATGCTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTAACATTCTCGAAATGCTCTGAGAGTATATGTGTTAAGCCTTCTTCGGAATCTTTTGTGttt                        >  2:1525195/1‑140 (MQ=255)

                                                                                                                 gAACATGCTGCGGT‑TGG‑ATGCTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTAACATTCTCGAAATGCTCTGAGAGTATATGTGTTAAGCCTTCTTCGGAATCTTTTGTGTTTGaaaa                   >  2:1828219/1‑140 (MQ=255)

                                                                                                                 gAACATGCTGCGGT‑TGG‑ATGCTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTAACATTCTCGAAATGCTCTGAGAGTATATGTGTTAAGCCTTCTTCGGAATCTTTTGTGTTTGaaaa                   >  2:4406282/1‑140 (MQ=255)

                                                                                                                  aaCATGCTGCGGT‑TGG‑ATGCTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTAACATTCTCGAAATGCTCTGAGAGTATATGTGTTAAGCCTTCTTCGGAATCTTTTGTGTTTGAAAAg                  >  2:4005551/1‑140 (MQ=255)

                                                                                                                  aaCATGCTGCGGT‑TGG‑ATGCTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTAACATTCTCGAAATGCTCTGAGAGTATATGTGTTAAGCCTTCTTCGGAATCTTTTGTGTTTGAAAAg                  >  1:5542194/1‑140 (MQ=255)

                                                                                                                  aaCATGCTGCGGT‑TGG‑ATGCTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTAACATTCTCGAAATGCTCTGAGAGTATATGTGTTAAGCCTTCTTCGGAATCTTTTGTGTTTGAAAAg                  >  2:6308013/1‑140 (MQ=255)

                                                                                                                  aaCATGCTGCGGT‑TGG‑ATGCTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTAACATTCTCGAAATGCTCTGAGAGTATATGTGTTAAGCCTTCTTCGGAATCTTTTGTGTTTGAAAAg                  >  1:855032/1‑140 (MQ=255)

                                                                                                                      tgctgcGGT‑TGG‑ATGCTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTAACATTCTCGAAATGCTCTGAGAGTATATGTGTTAAGCCTTCTTCGGAATCTTTTGTGTTTGAAAAGATGc              <  1:3604338/140‑1 (MQ=255)

                                                                                                                          gcGGT‑TGG‑ATGCTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTAACATTCTCGAAATGCTCTGAGAGTATATGTGTTAAGCCTTCTTCGGAATCTTTTGTGTTTGAAAAGATGCCttt          <  1:5735770/140‑1 (MQ=255)

                                                                                                                          gcGGT‑TGG‑ATGCTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTAACATTCTCGAAATGCTCTGAGAGTATATGTGTTAAGCCTTCTTCGGAATCTTTTGTGTTTGAAAAGATGCCttt          <  1:2266012/140‑1 (MQ=255)

                                                                                                                          gcGGT‑TGG‑ATGCTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTAACATTCTCGAAATGCTCTGAGAGTATATGTGTTAAGCCTTCTTCGGAATCTTTTGTGTTTGAAAAGATGCCttt          <  1:1033283/140‑1 (MQ=255)

                                                                                                                          gcGGT‑TGG‑ATGCTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTAACATTCTCGAAATGCTCTGAGAGTATATGTGTTAAGCCTTCTTCGGAATCTTTTGTGTTTGAAAAGATGCCttt          <  1:9208935/140‑1 (MQ=255)

                                                                                                                          gcGGT‑TGG‑ATGATATTTTTTTCCTGACGCTGAAAACATTACTAAAGTACCTTGTACCTTGGTTTTATCATTCTCGAAATGCTCTGAGAGTATATGTGTTAAGCCTTCTTCGGAATCTTTTGTGTTTGAAAAGATGCCttt          <  2:7088378/140‑1 (MQ=255)

                                                                                                                           cGGT‑TGG‑ATGCTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTATCATTCTCGATATGCTCTGAGAGTATATGTGTTAAGCCTTCTTCGGAATCTTTTGTGTTTGAAAAGATGCCttgc         >  1:6443070/1‑138 (MQ=255)

                                                                                                                              gttgg‑ATGCTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTAACATTCTCGAAATGCTCTGAGAGTATATGTGTTAAGCCTTCTTCGGAATCTTTTGTGTTTGAAAAGATGCCTTTCTg       >  1:8327944/5‑140 (MQ=255)

ATATCGGGTGTTGCTGAACCGTGTTGATACATCAACTGCACCTGATATTGAGTGGCCTACGAACCCTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGCGGT‑T‑G‑ATGTTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTAACATTCTCGAAATGCTCTGAGAGTATATGTGTTAAGCCTTCTTCGGAATCTTTTGTGTTTGAAAAGATGCCTTTCTGATTGT  >  NZ_CP025268/581069‑581337

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`