BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP025268	990,729	A→G	183 (TAA→TAG)	mepK →	YcbK family protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP025268	990,729	0	A	G	99.7%	2541.2 / NA	725	183 (TAA→TAG)	mepK	YcbK family protein
Reads supporting (aligned to +/- strand): ref base A (0/0); major base G (421/302); minor base T (2/0); total (423/302)
Fisher's exact test for biased strand distribution p-value = 5.13e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 736 total aligned reads displayed.

GCGATGGATTTCCATATTGAAGGTATCGCGTTAAGCAATATTCGCAAAGCCGCGTTATCTATGCGCGCAGGTGGTGTAGGATATTATCCACGTAGTAACTTTGTGCATATTGATACCGGGCCAGCACGGCACTGGTAATAATTGCTTAACGAAACAGGGGCAGTATGAACTATCGTATTATTCCGGTCACCGCATTCTCCCAGAACTGTTCATTAATCTGGTGTGAACAAACCCGTCTGGCCGCACTGGTCGATCCTGGCGGCGATGCGGAAAA  >  NZ_CP025268/990592‑990865

    tGGATTTCCATATTGAAGGTATCGCGTTAAGCAATATTCGCAAAGCCGCGTTATCTATGCGCGCAGGTGGTGTAGGATATTATCCACGTAGTAACTTTGTGCATATTGATACCGGGCCAGCACGGCACTGGTAGTAATTg                                                                                                                                    <  1:6796708/140‑1 (MQ=255)

    tGGATTTCCATATTGAAGGTATCGCGTTAAGCAATATTCGCAAAGCCGCGTTATCTATGCGCGCAGGTGGTGTAGGATATTATCCACGTAGTAACTTTGTGCATATTGATACCGGGCCAGCACGGCACTGGTAGTAATTg                                                                                                                                    >  1:7700604/1‑140 (MQ=255)

         ttCCATATTGAAGGTATCGCGTTAAGCAATATTCGCAAAGCCGCGTTATCTATGCGCGCAGGTGGTGTAGGATATTATCCACGTAGTAACTTTGTGCATATTGTTACCGGGCCAGCACGGCACTGGTAGTAATTGCTTaa                                                                                                                               >  2:7698828/1‑140 (MQ=255)

          tCCATATTGAAGGTATCGCGTTAAGCAATATTCGCAAAGCCGCGTTATCTCTGCGCGCAGGTGGTGTAGGATATTATCCACGTAGTAACTTTGTGCATATTGATACCGGGCCAGCACGGCACTGGTAGTAATTGCTTAAc                                                                                                                              >  2:5986932/1‑140 (MQ=255)

          tCCATATTGAAGGTATCGCGTTAAGCAATATTCGCAAAGCCGCGTTATCTATGCGCGCAGGTGGTGTAGGATATTATCCACGTAGTAACTTTGTGCATATTGATACCGGGCCAGCACGGCACTGGTAGTAATTGCTTAAc                                                                                                                              <  1:4966939/140‑1 (MQ=255)

          tCCATATTGAAGGTATCGCGTTAAGCAATATTCGCAAAGCCGCGTTATCTATGCGCGCAGGTGGTGTAGGATATTATCCACGTAGTAACTTTGTGCATATTGATACCGGGCCAGCACGGCACTGGTAGTAATTGCTTAAc                                                                                                                              >  1:6086690/1‑140 (MQ=255)

                  gAAGGTATCGCGTTAAGCAATATTCGCAAAGCCGCGTTATCTATGCGCGCAGGTGGTGTAGGATATTATCCACGTAGTAACATTGTGAATATTGATACCGGGCCAGCACGGCACTGGTAGTAATTGCTTAACGAAACAgg                                                                                                                      >  2:6418950/1‑140 (MQ=255)

                   aaGGTATCGCGTTAAGCAATATTCGCAAAGCCGCGTTATCTATGCGCGCAGGTGGTGTAGGATATTATCCACGTAGTAACTTTGTGCATATTGATACCGGGCCAGCACGGCACTGGTAGTAATTGCTTAACGAAACAggg                                                                                                                     <  1:6988049/140‑1 (MQ=255)

                    aGGTATCGCGTTAAGCAATATTCGCAAAGCCGCGTTATCTATGCGCGCAGGTGGTGTAGGATATTATCCACGTAGTAACTTTGTGCATATTGATACCGGGCCAGCACGGCACTGGTAGTAATTGCTTAACGAAACAgggg                                                                                                                    <  2:931374/140‑1 (MQ=255)

                    aGGTATCGCGTTAAGCAATATTCGCAAAGCCGCGTTATCTATGCGCGCAGGTGGTGTAGGATATTATCCACGTAGTAACTTTGTGCATATTGATACCGGGCCAGCACGGCACTGGTAGTAATTGCTTAACGAAACAgggg                                                                                                                    <  1:4775968/140‑1 (MQ=255)

                    aGGTATCGCGTTAAGCAATATTCGCAAAGCCGCGTTATCTATGCGCGCAGGTGGTGTAGGATATTATCCACGTAGTAACTTTGTGCATATTGATACCGGGCCAGCACGGCACTGGTAGTAATTGCTTAACGAAACAgggg                                                                                                                    <  1:7026452/140‑1 (MQ=255)

                    aGGTATCGCGTTAAGCAATATTCGCAAAGCCGCGTTATCTATGCGCGCAGGTGGTGTAGGATATTATCCACGTAGTAACTTTGTGCATATTGATACCGGGCCAGCACGGCACTGGTAGTAATTGCTTAACGAAACAgggg                                                                                                                    <  1:364819/140‑1 (MQ=255)

                    aGGTATCGCGTTAAGCAATATTCGCAAAGCCGCGTTATCTATGCGCGCAGGTGGTGTAGGATATTATCCACGTAGTAACTTTGTGCATATTGATACCGGGCCAGCACGGCACTGGTAGTAATTGCTTAACGAAACAgggg                                                                                                                    <  1:9088960/140‑1 (MQ=255)

                    aGGTATCGCGTTAAGCAATATTCGCAAAGCCGCGTTATCTATGCGCGCAGGTGGTGTAGGATATTATCCACGTAGTAACTTTGTGCATATTGATACCGGGCCAGCACGGCACTGGTAGTAATTGCTTAACGAAACAgggg                                                                                                                    <  2:260745/140‑1 (MQ=255)

                    aGGTATCGCGTTAAGCAATATTCGCAAAGCCGCGTTATCTATGCGCGCAGGTGGTGTAGGATATTATCCACGTAGTAACTTTGTGCATATTGATACCGGGCCAGCACGGCACTGGTAGTAATTGCTTAACGAAACAgggg                                                                                                                    <  2:4457528/140‑1 (MQ=255)

                    aGGTATCGCGTTAAGCAATATTCGCAAAGCCGCGTTATCTATGCGCGCAGGTGGTGTAGGATATTATCCACGTAGTAACTTTGTGCATATTGATACCGGGCCAGCACGGCACTGGTAGTAATTGCTTAACGAAACAgggg                                                                                                                    <  2:4592876/140‑1 (MQ=255)

                    aGGTATCGCGTTAAGCAATATTCGCAAAGCCGCGTTATCTATGCGCGCAGGTGGTGTAGGATATTATCCACGTAGTAACTTTGTGCATATTGATACCGGGCCAGCACGGCACTGGTAGTAATTGCTTAACGAAACAgggg                                                                                                                    <  1:2279732/140‑1 (MQ=255)

                    aGGTATCGCGTTAAGCAATATTCGCAAAGCCGCGTTATCTATGCGCGCAGGTGGTGTAGGATATTATCCACGTAGTAACTTTGTGCATATTGATACCGGGCCAGCACGGCACTGGTAGTAATTGCTTAACGAAACAgggg                                                                                                                    <  2:5635332/140‑1 (MQ=255)

                      gTATCGCGTTAAGCAATATTCGCAAAGCCGCGTTATCTATGCGCGCAGGTGGTGTAGGATATTATCCACGTAGTAACTTTGTGCATATTGATACCGGGCCAGCACGGCACTGGTAGTAATTGCTTAACGAAACAGGGGCa                                                                                                                  <  1:2049118/140‑1 (MQ=255)

                           gcgTTAAGCAATATTCGCAAAGCCGCGTTATCTATGCGCGCAGGTGGTGTAGGATATTATCCACGTAGTAACTTTGTGCATATTGATACCGGGCCAGCACGGCACTGGTAGTAATTGCTTAACGAAACAGGGGCAGTATg                                                                                                             <  2:7004293/140‑1 (MQ=255)

                             gTTAAGCAATATTCGCAAAGCCGCGTTATCTATGCGCGCAGGTGGTGTAGGATATTATCCACGTAGTAACTTTGTGCATATTGATACCGGGCCAGCACGGCACTGGTAGTAATTGCTTAACGAAACAGGGGCAGTATGaa                                                                                                           <  1:2995305/140‑1 (MQ=255)

                              ttAAGCAATATTCGCAAAGCCGCGTTATCTATGCGCGCAGGTGGTGTAGGATATTATCCACGTAGTAACTTTGTGCATATTGATACCGGGCCAGCACGGCACTGGTAGTAATTGCTTAACGAAACAGGGGCAGTATGAAc                                                                                                          <  2:4277982/140‑1 (MQ=255)

                                aaGCAATATTCGCAAAGCCGCGTTATCTATGCGCGCAGGTGGTGTAGGATATTATCCACGTAGTAACTTTGTGCATATTGATACCGGGCCAGCACGGCACTGGTAGTAATTGCTTAACGAAACAGCGGCAGTATGAACTa                                                                                                        <  2:8268894/140‑1 (MQ=255)

                                 aGCAATATTCGCAAAGCCGCGTTATCTATGCGCGCAGGTGGTGTAGGATATTATCCACGTAGTAACTTTGTGCATATTGATACCGGGCCAGCACGGCACTGGTAGTAATTGCTTAACGAAACAGGGGCAGTATGAACTAt                                                                                                       >  1:4436408/1‑140 (MQ=255)

                                 aGCAATATTCGCAAAGCCGCGTTATCTATGCGCGCAGGTGGTGTAGGATATTATCCACGTAGTAACTTTGTGCATATTGATACCGGGCCAGCACGGCACTGGTAGTAATTGCTTAACGAAACAGGGGCAGTATGAACTAt                                                                                                       >  1:4167936/1‑140 (MQ=255)

                                      tatTCGCAAAGCCGCGTTATCTATGCGCGCAGGTGGTGTAGGATATTATCCACGTAGTAACTTTGTGCATATTGATACCGGGCCAGCACGGCACTGGTAGTAATTGCTTAACGAAACAGGGGCAGTATGAACTATCGtat                                                                                                  >  1:2616674/1‑140 (MQ=255)

                                      tatTCGCAAAGCCGCGTTATCTATGCGCGCAGGTGGTGTAGGATATTATCCACGTAGTAACTTTGTGCATATTGATACCGGGCCAGCACGGCACTGGTAGTAATTGCTTAACGAAACAGGGGCAGTATGAACTATCGtat                                                                                                  >  1:7853753/1‑140 (MQ=255)

                                           gCAAAGCCGCGTTATCTATGCGCGCAGGTGGTGTAGGATATTATCCACGTAGTAACTTTGTGCATATTGATACCGGGCCAGCACGGCACTGGTAGTAATTGCTTAACGAAACAGGGGCAGTATGAACTATCGTATTATTc                                                                                             >  2:3687261/1‑140 (MQ=255)

                                             aaaGCCGCGTTATCTATGCGCGCAGGTGGTGTAGGATATTATCCACGTAGTAACTTTGTGCATATTGATACCGGGCCAGCACGGCACTGGTAGTAATTGCTTAACGAAACAGGGGCAGTATGAACTATCGTATTATTCCg                                                                                           >  2:3092572/1‑140 (MQ=255)

                                               aGCCGCGTTATCTATGCGCGCAGGTGGTGTAGGATATTATCCACGTAGTAACTTTGTGCATATTGATACCGGGCCAGCACGGCACTGGTAGTAATTGCTTAACGAAACAGGGGCAGTATGAACTATCGTATTATTCCGGt                                                                                         <  1:317302/140‑1 (MQ=255)

                                                gCCGCGTTATCTATGCGCGCAGGTGGTGTAGGATATTATCCACGTAGTAACTTTGTGCATATTGATACCGGGCCAGCACGGCACTGGTAGTAATTGCTTAACGAAACAGGGGCAGTATGAACTATCGTATTATTCCGGTc                                                                                        >  2:3903649/1‑140 (MQ=255)

                                                      ttATCTATGCGCGCAGGTGGTGTAGGATATTATCCACGTAGTAACTTTGTGCATATTGATACCGGGCCAGCACGGCACTGGTAGTAATTGCTTAACGAAACAGGGGCAGTATGAACTATCGTATTATTCCGGTCACCGCa                                                                                  <  1:5630669/140‑1 (MQ=255)

                                                      ttATCTATGCGCGCAGGTGGTGTAGGATATTATCCACGTAGTAACTTTGTGCATATTGATACCGGGCCAGCACGGCACTGGTAGTAATTGCTTAACGAAACAGGGGCAGTATGAACTATCGTATTATCCCGGTCACCGCa                                                                                  <  2:3379690/140‑1 (MQ=255)

                                                       tATCTATGCGCGCAGGTGGTGTAGGATATTATCCACGTAGTAACTTTGTGCATATTGATACCGGGCCAGCACGGCACTGGTAGTAATTGCTTAACGAAACAGGGGCAGTATGAACTATCGTATTATTCCGGTCACCGCAt                                                                                 <  1:986723/140‑1 (MQ=255)

                                                       tATCTATGCGCGCAGGTGGTGTAGGATATTATCCACGTAGTAACTTTGTGCATATTGATACCGGGCCAGCACGGCACTGGTAGTAATTGCTTAACGAAACAGGGGCAGTATGAACTATCGTATTATTCCGGTCACCGCAt                                                                                 <  1:8765669/140‑1 (MQ=255)

                                                       tATCTATGCGCGCAGGTGGTGTAGGATATTATCCACGTAGTAACTTTGTGCATATTGATACCGGGCCAGCACGGCACTGGTAGTAATTGCTTAACGAAACAGGGGCAGTATGAACTATCGTATTATTCCGGTCACCGCAt                                                                                 <  2:1756889/140‑1 (MQ=255)

                                                       tATCTATGCGCGCAGGTGGTGTAGGATATTATCCACGTAGTAACTTTGTGCATATTGATACCGGGCCAGCACGGCACTGGTAGTAATTGCTTAACGAAACAGGGGCAGTATGAACTATCGTATTATTCCGGTCACCGCAt                                                                                 <  1:3932610/140‑1 (MQ=255)

                                                       tATCTATGCGCGCAGGTGGTGTAGGATATTATCCACGTAGTAACTTTGTGCATATTGATACCGGGCCAGCACGGCACTGGTAGTAATTGCTTAACGAAACAGGGGCAGTATGAACTATCGTATTATTCCGGTCACCGCAt                                                                                 <  1:663022/140‑1 (MQ=255)

                                                       tATCTATGCGCGCAGGTGGTGTAGGATATTATCCACGTAGTAACTTTGTGCATATTGATACCGGGCCAGCACGGCACTGGTAGTAATTGCTTAACGAAACAGGGGCAGTATGAACTATCGTATTATTCCGGTCACCGCAt                                                                                 <  1:6242022/140‑1 (MQ=255)

                                                       tATCGATGCGCGCAGGTGGAGTAGGATATTATCCACGTAGTAACTGTGTGCATATTGATACCGGGCCAGCACGGCACTGGTAGTAATTGCTTAACGAAACAGGGGCAGTATGAACTATCGTATTATTCCGGTCACCGCag                                                                                 <  2:65893/140‑2 (MQ=255)

                                                         tCTATGCGCGCAGGTGGTGTAGGATATTATCCACGTAGTAACTTTGTGCATATTGATACCGGGCCAGCACGGCACTGGTAGTAATTGCTTAACGAAACAGGGGCAGTATGAACTATCGTATTATTCCGGTCACCGCATtc                                                                               <  1:9205849/140‑1 (MQ=255)

                                                             tGCGCGCAGGTGGTGTAGGATATTATCCACGTAGTAACTTTGTGCATATTGATACCGGGCCAGCACGGCACTGGTAGTAATTGCTTAACGAAACAGGGGCAGTATGAACTATCGTATTATTCCGGTCACCGCATTCTccc                                                                           >  2:6823606/1‑140 (MQ=255)

                                                              gcgcgcAGGTGGTGTAGGATATTATCCACGTAGTAACTTTGTGCATATTGATACCGGGCCAGCACGGCACTGGTAGTAATTGCTTAACGAAACAGGGGCAGTATGAACTATCGTATTATTCCGGTCACCGCATTCTCCCa                                                                          >  1:1491895/1‑140 (MQ=255)

                                                               cgcgcAGGTGGTGTAGGATATTATCCACGTAGTAACTTTGTGCATATTGATACCGGGCCAGCACGGCACTGGTAGTAATTGCTTAACGAAACAgggg                                                                                                                    >  2:5492300/1‑97 (MQ=255)

                                                                 cgcAGGTGGTGTAGTATATTATCCACGTAGTAACTTTGTGCATATTGATACCGGGCCAGCACGGCACTGGTAGTAATTGCTTAACGAAACAGGGGCAGTATGAACTATCGTATTATTCCGGTCAACGCATTCTCCCAGaa                                                                       <  1:8597394/140‑1 (MQ=255)

                                                                 cgcAGGTGGTGTAGGATATTATCCACGTAGTAACTTTGTGCATATTGATACCGGGCCAGCACGGCACTGGTAGTAATTGCTTAACGAAACAGGGGCAGTATGAACTATCGTATTATTCCGGTCACCGCATTCTCCCAGaa                                                                       >  2:1938510/1‑140 (MQ=255)

                                                                 cgcAGGTGGTGTAGGATATTATCCACGTAGTAACTTTGTGCATATTGATACCGGGCCAGCACGGCACTGGTAGTAATTGCTTAACGAAACAGGGGCAGTATGAACTATCGTATTATTCCGGTCACCGCATTCTCCCAGaa                                                                       >  2:8159186/1‑140 (MQ=255)

                                                                  gcAGGTGGTGTAGGATATTATCCACGTAGTAACTTTGTGCATATTGATACCGGGCCAGCACGGCACTGGTAGTAATTGCTTAACGAAACAGGGGCAGTATGAACTATCGTATTATTCCGGTCACCGCATTCTCCCAGAAc                                                                      <  1:1953053/140‑1 (MQ=255)

                                                                   catgtggtgTAGGATAATATACACTTATTAAAATTGAGAATATAGATACCCTTACAGCACGGCACTGGTCGAAAATGCTTAACGAAACAGGGGCAGTATGAACTAGCGTATTATTCCGGTCACCGCATTCTCCCAGAACt                                                                     >  2:4751323/4‑140 (MQ=255)

                                                                   cAGGTGGTGTAGGATATTATCCACGTAGTAACTTTGTGCATATTGATACCGGGCCAGCACGGCACTGGTAGTAATTGCTTAACGAAACAGGGGCAGTATg                                                                                                             >  1:5182762/1‑100 (MQ=255)

                                                                   cAGGTGGTGTAGGATATTATCCACGTAGTAACTTTGTGCATATTGATACCGGGCCAGCACGGCACTGGTAGTAATTGCTTAACGAAACAGGGGCAGTATGAACTATCGTATTATTCCGGTCACCGCATTCTCCCAGaa                                                                       <  1:7276873/138‑1 (MQ=255)

                                                                    aGGTGGTGTAGGATATTATCCACGTAGTAACTTTGTGCATATTGATACCGGGCCAGCACGGCACTGGTAGTAATTGCTTAACGAAACAGGGGCAGTATGAACTATCGTATTATTCCGGTCACCGCATTCTCCCAGAACTg                                                                    <  1:4676157/140‑1 (MQ=255)

                                                                          tgtAGGATATTATCCACGTAGTAACTTTGTGCATATTGATACCGGGCCAGCACGGCACTGGTAGTAATTGCTTAACGAAACAGGGGCAGTATGAACTATCGTATTATTCCGGTCACCGCATTCTCCCAGAACTGTTCAtt                                                              <  2:2113432/140‑1 (MQ=255)

                                                                                atatTATCCACGTAGTAACTTTGTGCATATTGATACCGGGCCAGCACGGCACTGGTAGTAATTGCTTAACGAAACAGGGGCAGTATGAACTATCGTATTATTCCGGTCACCGCATTCTCCCAGAACTGTTCATTAATc                                                          <  2:7153409/138‑1 (MQ=255)

                                                                                atatTATCCACGTAGTAACTTTGTGCATATTGATACCGGGCCAGCACGGCACTGGTAGTAATTGCTTAACGAAACAGGGGCAGTATGAACTATCGTATTATTCCGGTCACCGCATTCTCCCAGAACTGTTCATTAATCTg                                                        >  1:278969/1‑140 (MQ=255)

                                                                                 tattatCCACGTAGTAACTTTGTGCATATTGATACCGGGCCAGCACGGCACTGGTAGTAATTGCTTAACGAAACAGGGGCAGTATGAACTATCGTATTATTCCGGTCACCGCATTCTCCCAGAACTGTTCATTAATCTgg                                                       >  2:1422252/1‑140 (MQ=255)

                                                                                 tattatCCACGTAGTAACGTTGTGCATATTGATACCGGGCCAGCACGGCACTGGTAGTAATTGCTTAACGAAACAGGGGCAGTATGAACTATCGTATTATTCCGGTCACCGCATTCTCCCAGAACTGTTCATTAATCTgg                                                       >  2:6094320/1‑140 (MQ=255)

                                                                                    tatCCACGTAGTAACTTTGTGCATATTGATACCGGGCCAGCACGGCACTGGTAGTAATTGCTTAACGAAACAGGGGCAGTATGAACTATCGTATTATTCCGGTCAGCGCATTCTCCCAGAACTGTTCATTAATCTGgtgt                                                    >  2:5264418/1‑140 (MQ=255)

                                                                                    tatCCACGTAGTAACTTTGTGCATATTGATACCGGGCCAGCACGGCACTGGTAGTAATTGCTTAACGAAACAGGGGCAGTATGAACTATCGTATTATTCCGGTCACCGCAt                                                                                 <  2:8688510/111‑1 (MQ=255)

                                                                                    tatCCACGTAGTAACTTTGTGCATATTGATACCGGGCCAGCACGGCACTGGTAGTAATTGCTTAACGAAACAGGGGCAGTATGAACTATCGTATTATTCCGGTCACCGCATTCTCCCAGAACTGTTCATTAATCTGgtgt                                                    >  2:1237840/1‑140 (MQ=255)

                                                                                    tatCCACGTAGTAACTTTGTGCATATTGATACCGGGCCAGCACGGCACTGGTAGTAATTGCTTAACGAAACAGGGGCAGTATGAACTATCGTATTATTCCGGTCACCGCATTCTCCCAGAACTGTTCATTAATCTGgtgt                                                    >  1:210232/1‑140 (MQ=255)

                                                                                    tatCCACGTAGTAACTTTGTGCATATTGATACCGGGCCAGCACGGCACTGGTAGTAATTGCTTAACGAAACAGGGGCAGTATGAACTATCGTATTATTCCGGTCACCGCATTCTCCCAGAACTGTTCATTAATCTGgtgt                                                    >  1:1614380/1‑140 (MQ=255)

                                                                                    tatCCACGTAGTAACTTTGTGCATATTGATACCGGGCCAGCACGGCACTGGTAGTAATTGCTTAACGAAACAGGGGCAGTATGAACTATCGTATTATTCCGGTCACCGCATTCTCCCAGAACTGTTCATTAATCTGgtgt                                                    >  2:9175122/1‑140 (MQ=255)

                                                                                    tatCCACGTAGTAACTTTGTGCATATTGATACCGGGCCAGCACGGCACTGGTAGTAATTGCTTAACGAAACAGGGGCAGTATGAACTATCGTATTATTCCGGTCACCGCATTCTCCCAGAACTGTTCATTAATCTGgtgt                                                    >  1:5744318/1‑140 (MQ=255)

                                                                                    tatCCACGTAGTAACGTTGTGCATATTGATACCGGGCCAGCACGGCACTGGTAGTAATTGCTTAACGAAACAGGGGCAGTATGAACTCTCGTATTATTCCGGTCACCGCATTCTCCCAGACCTGGTCATTAATCTGgtgt                                                    >  2:7591412/1‑140 (MQ=255)

                                                                                      tCCACGTAGTAACTTTGTGCATATTGATACCGGGCCAGCACGGCACTGGTCGTAATTGCTTAACGAAACAGGGGCAGTATGAACTATCGTATTATTCCGGTCACCGCATTCTCCCAGACCTGTTCAGTAATCTGGTGTGa                                                  >  1:8156789/1‑140 (MQ=255)

                                                                                      tCCACGTAGTAACTTTGTGCATATTGATACCGGGCCAGCACGGCACTGGTAGTAATTGCTTAACGAAACAGGGGCAGTATGAACTATCGTATTATTCCGGTCACCGCATTCTCCCAGAACTGTTCATTAATCTGGTGTGa                                                  >  1:7125994/1‑140 (MQ=255)

                                                                                      tCCACGTAGTAACTTTGTGCATATTGATACCGGGCCAGCACGGCACTGGTAGTAATTGCTTAACGAAACAGGGGCAGTATGAACTATCGTATTATTCCGGTCACCGCATTCTCCCAGAACTGTTCATTAATCTGGTGTGa                                                  >  1:7586390/1‑140 (MQ=255)

                                                                                      tCCACGTAGTAACTTTGTGCATATTGATACCGGGCCAGCACGGCACTGGTAGTAATTGCTTAACGAAACAGGGGCAGTATGAACTATCGTATTATTCAGGTCACCGCATTCTCCCAGAACTGTTCATTAATCTGGTGTGa                                                  >  2:578971/1‑140 (MQ=255)

                                                                                         aCGTAGTAACTTTGTGCATATTGATACCGGGCCAGCACGGCACTGGTAGTAATTGCTTAACGAAACAGGGGCAGTATGAACTATCGTATTATTCCGGTCACCGCATTCTCCCAGAACTGTTCATTAATCTGGTGTGAACa                                               >  2:2947938/1‑140 (MQ=255)

                                                                                         aCGTAGTAACTTTGTGCATATTGATACCGGGCCAGCACGGCACTGGTAGTAATTGCTTAACGAAACAGGGGCAGTATGAACTATCGTATTATTCCGGTCACCGCATTCTCCCAGAACTGTTCATTAATCTGGTGTGAACa                                               >  1:5096320/1‑140 (MQ=255)

                                                                                         aCGTAGTAACTTTGTGCATATTGATACCGGGCCAGCACGGCACTGGTAGTAATTGCTTAACGAAACAGGGGCAGTATGAACTATCGTATTATTCCGGTCACCGCATTCTCCCAGAACTGTTCAGTAATCTGGTGTGAACa                                               >  2:4953934/1‑140 (MQ=255)

                                                                                           gtagtaACTTTGTGCATATTGATACCGGGCCAGCACGGCACTGGTAGTAATTGCTTAACGAAACAGGGGCAGTATGaa                                                                                                           >  1:3792425/1‑78 (MQ=255)

                                                                                           gtagtaACTTTGTGCATATTGATACCGGGCCAGCACGGCACTGGTAGTAATTGCTTAACGAAACAGGGGCAGTATGAACTATCGTATTATTCCGGTCACCGCATTCTCCCAGAACTGTTCATTAATCTGGTGTGAACaaa                                             >  2:2374956/1‑140 (MQ=255)

                                                                                             agtaACTTTGTGCATATTGATACCGGGCCAGCACGGCACTGGTAGTAATTGCTTAACGAAACAGGGGCAGTATGAACTATCGTATTATTCCGGTCACCGCATTCTCCCAGAACTGTTCATTAATCTGGTGTGAACAAAcc                                           >  2:2779290/1‑140 (MQ=255)

                                                                                             agtaACTTTGTGCATATTGATACCGGGCCAGCACGGCACTGGTAGTAATTGCTTAACGAAACAGGGGCAGTATGAACTATCGTATTATTCCGGTCACCGCATTCTCCCAGAACTGTTCATTAATCTGGTGTGAACAAAcc                                           >  1:7449615/1‑140 (MQ=255)

                                                                                              gtaACTTTGTGCATATTGATACCGGGCCAGCACGGCACTGGTAGTAATTGCTTAACGAAACAGGGGCAGTATGAACTATCGTATTATTCCGGTCACCGCATTCTCCCAGAACTGTTCATTAATCTGGTGTGAACAAAccc                                          >  1:8330387/1‑140 (MQ=255)

                                                                                               taACTTTGTGCATATTGATACCGGGCCAGCACGGCACTGGTAGTAATTGCTTAACGAAACAGGGGCAGTATGAACTATCGTATTATTCCGGTCACCGCATTCTCCCAGAACTGTTCATTAATCTGGTGTGAACAAACCCg                                         >  2:511082/1‑140 (MQ=255)

                                                                                               taACTTTGTGCATATTGATACCGGGCCAGCACGGCACTGGAATAAATAGATTAACGAAACATGGGAAGTATGAACTATCGTATTATTCCGGTCACCGAATTCTCACAGAAATGTACATTAAACTGGAGTTAACAAACCCg                                         >  2:3534319/1‑140 (MQ=255)

                                                                                                aaCTTTGTGCATATTGATACCGGGCCAGCACGGCACTGGTAGTAATTGCTTAACGAAACAGGGGCAGTATGAACTATCGTATTATTCCGGTCACCGCATTCTCCCAGAACTGTTCATTAATCTGGTGTGAACAAACCCGt                                        >  2:1132617/1‑140 (MQ=255)

                                                                                                  cTTTGTGCATATTGATACCGGGCCAGCACGGCACTGGTAGTAATTGCTTAACGAAACAGGGGCAGTATGAACTATCGTATTATTCCGGTCACCGCATTCTCCCAGAACTGTTCATTAATCTGGTGTGAACAAACCCGTCt                                      >  1:3517441/1‑140 (MQ=255)

                                                                                                     tgtgCATATTGATACCGGGCCAGCACGGCACTGGTAGTAATTGCTTAACGAAACAGGGGCAGTATGAACTATCTTATTATTCCGGTCACCGCATTCTCCCAGAACTGTTCATTAATCTGGTGTGAACAAACCCGTCTGGc                                   >  2:1322037/1‑140 (MQ=255)

                                                                                                     tgtgCATATTGATACCGGGCCAGCACGGCACTGGTAGTAATTGCTTAACGAAACAGGGGCAGTATGAACTATCGTATTATTCCGGTCACCGCATTCTCCCAGAACTGTTCATTAATCTGGTGTGAACAAACCCGTCTGGc                                   >  1:7971237/1‑140 (MQ=255)

                                                                                                       tgCATATTGATACCGGGCCAGCACGGCACTGGTAGTAATTGCTTAACGAAACAGGGGCAGTATGAACTATCGTATTATTCCGGTCACCGCATTCTCCCAGAACTGTTCATTAATCTGGTGTGAACAAACCCGTCTGGCCg                                 >  2:7895453/1‑140 (MQ=255)

                                                                                                       tgCATATTGATACCGGGCCAGCACGGCACTGGTAGTAATTGCTTAACGAAACAGGGGCAGTATGAACTATCGTATTATTCCGGTCACCGCATTCTCCCAGAACTGTTCATTAATCTGGTGTGAACAAACCCGTCTGGCCg                                 >  1:1109289/1‑140 (MQ=255)

                                                                                                       tgCATATTGATACCGGGCCAGCACGGCACTGGTAGTAATTGCTTAACGAAACAGGGGCAGTATGAACTATCGTATTATTCCGGTCACCGCATTCTCCCAGAACTGTTCATTAATCTGGTGTGAACAAACCCGTCTGGCCg                                 >  1:3376788/1‑140 (MQ=255)

                                                                                                            atTGATACCGGGCCAGCACGGCACTGGTAGTAATTGCTTAACGAAACAGGGGCAGTATGAACTATCGTATTATTCCGGTCACCGCATTCTCCCAGAACTGTTCATTAATCTGGTGTGAACAAACCCGTCTGGCCGCACTg                            >  2:4092138/1‑140 (MQ=255)

                                                                                                               gATACCGGGCCAGCACGGCACTGGTAGTAATTGCTTAACGAAACAGGGGCAGTATGAACTATCGTATTATTCCGGTCACCGCATTCTCCCAGAACTGTTCATTAATCTGGTGTGAACAAACCCGTCTGGCCGCACTGGTc                         <  1:1777135/140‑1 (MQ=255)

                                                                                                                 tACCGGGCCAGCACGGCACTGGTAGTAATTGCTTAACGAAACAGGGGCAGTATGAACTATCGTATTATTCCGGTCACCGCATTCTCCCAGAACTGTTCATTAATCTGGTGTGAACAAACCCGTCTGGCCGCACTGGTCGa                       >  1:9375365/1‑140 (MQ=255)

                                                                                                                 tACCGGGCCAGCACGGCACTGGTAGTAATTGCTTAACGAAACAGGGGCAGTATGAACTATCGTATTATTCCGGTCACCGCATTCTCCCAGAACTGTTCATTAATCTGGTGTGAACAAACCCGTCTGGCCGCACTGGTCGa                       >  2:8443749/1‑140 (MQ=255)

                                                                                                                      ggCCAGCACGGCACTGGTAGTAATTGCTTAACGAAACAGGGGCAGTATGAACTATCGTATTATTCCGGTCACCGCATTCTCCCAGAACTGTTCATTAATCTGGTGTGAACACACCCGTCTGGCCGCACTGGTCGATCCTg                  >  2:1543114/1‑140 (MQ=255)

                                                                                                                      ggCCAGCACGGCACTGGTAGTAATTGCTTAACGAAACAGGGGCAGTATGAACTATCGTATTATTCCGGTCACCGCATTCTCCCAGAACTGTTCATTAATCTGGTGTGAACAAACCCg                                         >  1:5538084/1‑117 (MQ=255)

                                                                                                                      ggCCAGCACGGCACTGGTAGTAATTGCTTAACGAAACAGGGGCAGTATGAACTATCGTATTATTCCGGTCACCGCATTCTCCCAGAACTGTTCATTAATCTGGTGTGAACAAACCCGTCTGGCCGCACTGGTCGATCCTg                  >  2:624263/1‑140 (MQ=255)

                                                                                                                        ccAGCACGGCACTGGTAGTAATTGCTTAACGAAACAGGGGCAGTATGAACTATCGTATTATTCCGGTCACCGCATTCTCCCAGAACTGTTCATTAATCTGGTGTGAACAAACCCGTCTGGCCGCACTGGTCGATCCTggc                >  1:1259186/1‑140 (MQ=255)

                                                                                                                            cACGGCACTGGTAGTAATTGCTTAACGAAACAGGGGCAGTATGAACTATCGTATTATTCCGGTCACCGCATTCTCCCAGAACTGTTCATTAATCTGGTGTGAACAAACCCGTCTGGCCGCACTGGTCGATCCTGgcggcg            >  2:7397214/1‑140 (MQ=255)

                                                                                                                            cACGGCACTGGTAGTAATTGCTTAACGAAACAGGGGCAGTATGAACTATCGTATTATTCCGGTCACCGCATTCTCCCAGAACTGTTCATTAATCTGGTGTGAACAAACCCGTCTGGCCGCACTGGTCGATCCTGgcggcg            >  2:8987237/1‑140 (MQ=255)

                                                                                                                              cGGCACTGGTAGTAATTGCTTAACGAAACAGGGGCAGTATGAACTATCGTATTATTCCGGTCACCGCATTCTCCCAGAACTGTTCATTAATCTGGTGTGAACAAACCCGTCTGGCCGCACTGGTCGATCCTGGCGGCGAt          >  1:8433940/1‑140 (MQ=255)

                                                                                                                                 cACTGGTAGTAATTGCTTAACGAAACAGGGGCAGTATGAACTATCGTATTATTCCGGTCACCGCAt                                                                                 <  1:4105764/66‑1 (MQ=255)

                                                                                                                                 cACTGGTAGTAATTGCTTAACGAAACAGGGGCAGTATGAACTATCGTATTATTCCGGTCACCGCATTCTCCCAGAACTGTTCATTAATCTGGTGTGAACAAACCCGTCTGGCCGCa                               >  1:6460238/1‑116 (MQ=255)

                                                                                                                                 cACTGGTAGTAATTGCTTAACGAAACAGGGGCAGTATGAACTATCGTATTATTCCGGTCACCGCATTCTCCCAGAACTGTTCATTAATCTGGTGTGAACAAACCCGTCTGGCCGCACTGGTCGATCCTGGCGGCGATGCg       >  2:5201424/1‑140 (MQ=255)

GCGATGGATTTCCATATTGAAGGTATCGCGTTAAGCAATATTCGCAAAGCCGCGTTATCTATGCGCGCAGGTGGTGTAGGATATTATCCACGTAGTAACTTTGTGCATATTGATACCGGGCCAGCACGGCACTGGTAATAATTGCTTAACGAAACAGGGGCAGTATGAACTATCGTATTATTCCGGTCACCGCATTCTCCCAGAACTGTTCATTAATCTGGTGTGAACAAACCCGTCTGGCCGCACTGGTCGATCCTGGCGGCGATGCGGAAAA  >  NZ_CP025268/990592‑990865

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`