BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP025268	1,570,577	T→C	461 (TAA→TAG)	dosC ←	diguanylate cyclase DosC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP025268	1,570,577	0	T	C	99.9%	2362.7 / NA	675	461 (TAA→TAG)	dosC	diguanylate cyclase DosC
Reads supporting (aligned to +/- strand): ref base T (0/0); major base C (312/362); minor base A (0/1); total (312/363)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 675 total aligned reads displayed.

TTGAAAAACATCACTTCATCATTTTCGTTAATTAACACCGCACCCATCATATTTTGCTCAAGGGCGGGG‑AAAAAAATGCCATCGGCGGCATTATCCGCATCGGTTAGCTTCATGATTACCTCTGCATCCTGGCGCATTTAAAGACTGGCTTTCCAGAGTTCAACACGGTTTCTACCTCGTCTTTTGGCGATATACAGAGCTTCATCGGCTATTTGAATGAGGCGCTCATAGTCAGGATGACCATTAAACATGGCGGCACCGATGGAAAGTGAGA  >  NZ_CP025268/1570440‑1570713

     aaaCATCACTTCATCATTTTCGTTACTTAACACCGCACCCATCATATTTTGCTCACGGGCGGGG‑AAAAAAATGCCATCGGCGGCATTATCCGCATCGGTTAGCTTCAGGATTACCTCTGCATCCTGGCGCATCTAAAGAc                                                                                                                                   <  2:5085409/140‑1 (MQ=255)

     aaaCATCACTTCATCATTTTCGTTAATTAACACCGCACCCATCATATTTTGCTCAAGGGCGGGG‑AAAAAAATGCCATCGGCGGCATTATCCGCATCGGTTAGCTTCATGATTACCTCTGCATCCTGGCGCATCTAAAGAc                                                                                                                                   <  1:7775759/140‑1 (MQ=255)

     aaaCATCACTTCATCATTTTCGTTAATTAACACCGCACCCATCATATTTTGCTCAAGGGCGGGG‑AAAAAAATGCCATCGGCGGCATTATCCGCATCGGTTAGCTTCATGATTACCTCTGCATCCTGGCGCATCTAAAGAc                                                                                                                                   <  1:3690328/140‑1 (MQ=255)

     aaaCATCACTTCATCATTTTCGTTAATTAACACCGCACCCATCATATTTTGCTCAAGGGCGGGG‑AAAAAAATGCCATCGGCGGCATTATCCGCATCGGTTAGCTTCATGATTACCTCTGCATCCTGGCGCATCTAAAGAc                                                                                                                                   <  1:3364987/140‑1 (MQ=255)

        cATCACTTCATCATTTTCGTTAATTAACACCGCACCCATCATATTTTGCTCAAGGGCGGGG‑AAAAAAATGCCATCGGCGGCATTATCCGCATCGGTTAGCTTCATGATTACCTCTGCATCCTGGCGCATCTAAAGACTgg                                                                                                                                >  1:2515977/1‑140 (MQ=255)

          tCACTTCATCATTTTCGTTAATTAACACCGCACCCATCATATTTTGCTCAAGGGCGGGG‑AAAAAAATGCCATCGGCGGCATTATCCGCATCGGTTAGCTTCATGATTACCTCTGCATCCTGGCGCATCTAAAGACTGGCt                                                                                                                              <  1:5259070/140‑1 (MQ=255)

           cACTTCATCATTTTCGTTAATTAACACCGCACCCATCATATTTTGCTCACGGGCGGGG‑AAAAAAATGCCATCGGCGGCATTATCCGCATCGGTTAGCTTCATGATTACCTCTGCATCCTGGCGCATCTAAAGACTGGCtt                                                                                                                             <  2:605773/140‑1 (MQ=255)

              ttCATCATTTTCGTTAATTAACACCGCACCCATCATATTTTGCTCAAGGGCGGGG‑AAAAAAATGCCATCGGCGGCATTATCCGCATCGGTTAGCTTCATGAGTACCTCTGCATCCTGGCGCATCTAAAGACTGGCTTTcc                                                                                                                          >  2:7502045/1‑140 (MQ=255)

               tcatcaTTTTCGTTAATTAACACCGCACCCATCATATTTTGCTCAAGGGCGGGG‑AAAAAAATGCCATCGGCGGCATTATCCGCATCGGTTAGCTTCATGATTACCTCTGCATCCTGGCGCATCTAAAGACTGGCTTTCCa                                                                                                                         <  2:9366820/140‑1 (MQ=255)

               ccatcatTGTCGTTAATTTGCACCGCACCCCTCATATTTTGCTCAAGGGCGGGG‑AAAAAAATGCCATCGGCGGCATTATCCGCATCGGTTAGCTTCATGATTACCTCTGCATCCTGGAGCATCTAAAGACTGGCTTTCCa                                                                                                                         <  2:8621182/139‑1 (MQ=255)

                 atcatTTTCGTTAATTAACACCGCACCCATCATATTTTGCTCAAGGGCGGGG‑AAAAAAATGCCATCGGCGGCATTATCCGCATCGGTTAGCTTCATGATTACCTCTGCATCCTGGCGCATCTAAAGACTGGCTTTCCaga                                                                                                                       <  1:5028565/140‑1 (MQ=255)

                   catTTTCGTTAATTAACACCGCACCCATCATATTTTGCTCAAGGGCGGGG‑AAAAAAATGCCATCGGCGGCATTATCCGCATCGGTTAGCTTCATGATTACCTCTGCATCCTGGCGCATCTAAAGACTGGCTTTCCAGAGt                                                                                                                     >  1:8560388/1‑140 (MQ=255)

                      tttCGTTAATTAACACCGCACCCATCATATTTTGCTCAAGGGCGGGG‑AAAAAAATGCCATCGGCGGCATTATCCGCATCGGTTAGCTTCATGATTACCTCTGCATCCTGGCGCATCTAAAGACTGGCTTTCCAGAGTTCa                                                                                                                  >  1:9368197/1‑140 (MQ=255)

                      tttCGTTAATTAACACCGCACCCATCATATTTTGCTCAAGGGCGGGG‑AAAAAAATGCCATCGGCGGCATTATCCGCATCGGTTAGCTTCATGATTACCTCTGCATCCTGGCGCATCTAAAGACTGGCTTTCCAGAGTTCa                                                                                                                  >  1:7091945/1‑140 (MQ=255)

                         cGTTAATTAACACCGCACCCATCATATTTTGCTCAAGGGCGGGG‑AAAAAAATGCCATCGGCGGCATTATCCGCATCGGTTAGCTTCATGATTACCTCTGCATCCTGGCGCATCTAAAGACTGGCTTTCCAGAGTTCAaca                                                                                                               <  2:6448485/140‑1 (MQ=255)

                                        cACCCATCATATTTTGCTCAAGGGCGGGG‑AAAAAAATGCCATCGGCGGCATTATCCGCATCGGTTAGCTTCATGATTACCTCTGCATCCTGGCGCATCTAAAGACTGGCTTTCCAGAGTTCAACACGGTTTCTACCTCgg                                                                                                >  2:5917537/1‑139 (MQ=255)

                                        cACCCATCATATTTTGCTCAAGGGCGGGG‑AAAAAAATGCCATCGGCGGCATTATCCGCATCGGTTAGCTTCATGATTACCTCTGCATCCTGGCGCATCTAAAGACTGGCTTTCCAGAGTTCAACACGGTTTCTACCTCGt                                                                                                >  1:2020279/1‑140 (MQ=255)

                                            catcatATTTTGCTCAAGGGCGGGG‑AAAAAAATGCCATCGGCGGCATTATCCGCATCGGTTAGCTTCATGATTACCTCTGCATCCTGGCGCATCTAAAGACTGGCTTTCCAGAGTTCAACACGGTTTCTACCTCGTCttt                                                                                            <  1:1739155/140‑1 (MQ=255)

                                            cagcatCTTTTGCTCACGGGCGGGG‑AAACAAATGCCATCCGCGGCATGATCCGCATCGGTTAGCTTCATGATTACCTCTGCATCCTGGCGCATCTAAAGACTGGCTTTCCAGAGTTCAACACGGTTTCGACCTCGTCttt                                                                                            <  2:216353/137‑1 (MQ=255)

                                             atcatATTTTGCTCAAGGGCGGGG‑AAAAAAATGCCATCGGCGGCATTATCCGCATCGGTTAGCTTCATGATTACCTCTGCATCCTGGCGCATCTAAAGACTGGCTTTCCAGAGTTCAACACGGTTTCTACCTCGTCtttt                                                                                           >  1:5718048/1‑140 (MQ=255)

                                             atcatATTTTGCTCAAGGGCGGGG‑AAAAAAATGCCATCGGCGGCATTATCCGCATCGGTTAGCTTCATGATTACCTCTGCATCCTGGCGCATCTAAAGACTGGCTTTCCAGAGTTCAACACGGTTTCTACCTCGTCtttt                                                                                           >  2:827232/1‑140 (MQ=255)

                                             atcatATTTTGCTCAAGGGCGGGG‑AAAAAAATGCCATCGGCGGCATTATCCGCATCGGTTAGCTTCATGATTACCTCTGCATCCTGGCGCATCTAAAGACTGGCTTTCCAGAGTTCAACACGGTTTCTACCTCGTCtttt                                                                                           >  1:3506542/1‑140 (MQ=255)

                                              tcatATTTTGCTCAAGGGCGGGG‑AAANAAATGCCNTCGGCGGCATTATCCGCATCGGTTAGCTTCATGATTACCTCTGCATCCTGGCGCATCTAAAGACTGGCTTTCCAGAGTTCAACANGGTTTCCACCTCGTCTTTTg                                                                                          <  1:6225091/140‑1 (MQ=255)

                                              tcatATTTTGCTCAAGGGCGGGG‑AAAAAAATGCCATCGGCGGCATTATCCGCATCGGTTAGCTTCATGATTACCTCTGCATCCTGGCGCATCTAAAGACTGGCTTTCCAGAGTTCAACACGGTTTCTACCTCGTCTTTTg                                                                                          <  2:5214262/140‑1 (MQ=255)

                                               catATTTTGCTCAAGGGCGGGGAAAAAAAATGCCATCGGCGGCATTATCCGCATCGGTTAGCTTCATGATTACCTCTGCATCCTGGCGCATCTAAAGACTGGCTTTCCAGAGTTCAACACGGTTTCTACCTCGTCTTTTg                                                                                          <  2:5634455/140‑1 (MQ=255)

                                               catATTTTGCTCAAGGGCGGGG‑AAAAAAATGCCATCGGCGGCATTATCCGCATCGGTTAGCTTCATGATTACCTCTGCATCCTGGCGCATCTAAAGACTGGCTTTCCAGAGTTCAACACGGTTTCTACCTCGTCTTTTgg                                                                                         <  1:2236994/140‑1 (MQ=255)

                                                 tatTTTGCTCAAGGGCGGGG‑AAAAAAATGCCATCGGCGGCATTATCCGCATCGGTTAGCTTCATGATTACCTCTGCATCCTGGCGCATCTAAAGACTGGCTTTCCAGAGTTCAACACGGTTTCTACCTCGTCTTTTGGCg                                                                                       <  1:4485336/140‑1 (MQ=255)

                                                 tatTTTGCTCAAGGGCGGGG‑AAAAAAATGCCATCGGCGGCATTATCCGCATCGGTTAGCTTCATGATTACCTCTGCATCCTGGCGCATCTAAAGACTGGCTTTCCAGAGTTCAACACGGTTTCTACCTCGTCTTTTGGCg                                                                                       <  2:1349678/140‑1 (MQ=255)

                                                 tatTTTGCTCAAGGGCGGGG‑AAAAAAATGCCATCGGCGGCATTAACCGCATCGGTTAGCTTCATGATTACCTCTGCATCCTGGCGCATCTAAAGACTGGCTTTCCAGAGTTCAACACGGTTTCTACCTCGTCTTTTGGCg                                                                                       <  2:7737478/140‑1 (MQ=255)

                                                     ttGCTCAAGGGCGGGG‑AAAAAAATGCCATCGGCGGCATTATCCGCATCGGTTAGCTTCATGATTACCTCTGCATCCTGGCGCATCTAAAGACTGGCTTTCCAGAGTTCAACACGGTTTCTACCTCGTCTTTTGGCGatat                                                                                   >  1:3864191/1‑140 (MQ=255)

                                                     ttGCTCAAGGGCGGGG‑AAAAAAATGCCATCGGCGGCATTATCCGCATCGGTTAGCTTCATGATTACCTCTGCATCCTGGCGCATCTAAAGACTGGCTTTCCAGAGTTCAACACGGTTTCTACCTCGTCTTTTGGCGatat                                                                                   >  2:7946428/1‑140 (MQ=255)

                                                      tGCTCAAGGGCGGGG‑AAAAAAATGCCATCGGCGGCATTATCCGCATCGGTTAGCTTCATGATTACCTCTGCATCCTGGCGCATCTAAAGACTGGCTTTCCAGAGTTCAACTCGGTTTCTACCTCGTCTTTTGGCGAtata                                                                                  >  2:4022044/1‑140 (MQ=255)

                                                        cTCAAGGGCGGGG‑AAAAAAATGCCATCGGCGGCATTATCCGCATCGGTTAGCTTCATGNTTACCTCTGCATCCTGGCGCATCTAAAGACTGGCTTTCCAGAGTTCAACACGGTTTCTACCTCGTCTTTTGGCGATATACa                                                                                >  1:6281109/1‑140 (MQ=255)

                                                        cTCAAGGGCGGGG‑AAAAAAATGCCATCGGCGGCATTATCCGCATCGGTTAGCTTCATGATTACCTCTGCATCCTGGCGCATCTAAAGACTGGCTTTCCAGAGTTCAACACGGTTTCTACCTCGTCTTTTGGCGATATACa                                                                                <  2:3441740/140‑1 (MQ=255)

                                                        cTCAAGGGCGGGG‑AAAAAAATGCCATCGGCGGCATTATCCGCATCGGTTAGCTTCATGATTACCTCTGCATCCTGGCGCATCTAAAGACTGGCTTTCCAGAGTTCAACACGGTTTCTACCTCGTCTTTTGGCGATATACa                                                                                >  2:1571169/1‑140 (MQ=255)

                                                        cTCAAGGGCGGGG‑AAAAAAATGCCATCGGCGGCATTATCCGCATCGGTTAGCTTCATGATTACCTCTGCATCCTGGCGCATCTAAAGACTGGCTTTCCAGAGTTCAACACGGTTTCTACCTCGTCTTTTGGCGATATACa                                                                                >  1:2098512/1‑140 (MQ=255)

                                                        cTCAAGGGCGGGG‑AAAAAAATGCCATCGGCGGCATTATCCGCATCGGTTAGCTTCATGATTACCTCTGCATCCTGGCGCATCTAAAGACTGGCTTTCCAGAGTTCAACACGGTTTCTACCTCGTCTATTGGCGATATACa                                                                                >  2:6830837/1‑140 (MQ=255)

                                                             gggCGGGG‑AAAAAAATGCCATCGGCGGCATTATCCGCATCGGTTAGCTTCATGATTACCTCTGCATCCTGGCGCATCTAAAGACTGGCTTTCCAGAGTTCAACACGGTTTCTACCTCGTCTTTTGGCGATATACAGAGCt                                                                           >  1:8695518/1‑140 (MQ=255)

                                                             gggCGGGG‑AAAAAAATGCCATCGGCGGCATTATCCGCATCGGTTAGCTTCATGATTACCTCTGCATCCTGGCGCATCTAAAGACTGGCTTTCCAGAGTTCAACACGGTTTCTACCTCGTCTTTTGGCGATATACAGAGCt                                                                           >  1:1549432/1‑140 (MQ=255)

                                                                  ggg‑AAAAAAATGCCATCGGCGGCATTATCCGCATCGGTTAGCTTCATGATTACCTCTGCATCCTGGCGCATCTAAAGACTGGCTTTCCAGAGTTCAACACGGTTTCTACCTCGTCTTTTGGCGATATACAGAGCTTCATc                                                                      >  2:6271466/1‑140 (MQ=255)

                                                                  ggg‑AAAAAAATGCCATCGGCGGCATTATCCGCATCGGTTAGCTTCATGATTACCTCTGCATCCTGGCGCATCTAAAGACTGGCTTTCCAGAGTTCAACACGGTTTCTACCTCGTCTTTTGGCGATATACAGAGCTTCATc                                                                      <  1:7642541/140‑1 (MQ=255)

                                                                    g‑AAAACAATGCCCTCGGCGGCATTATCCGCATCGGTTCGCCTCATGATGACCGCTGCATCCTGGCGCATCTACAGACTGGCTTTCCAGAGTTCACCACGGTTTCTACCTCGTCTTTTGGCGATATACAGAGCTTCATCgg                                                                    <  2:1196663/140‑1 (MQ=255)

                                                                    g‑AAAAAAATGCCATCGGCGGCATTATCCGCATCGGTTAGCTTCATGATTACCTCTGCATCCTGGCGCATCTAAAGACTGGCTTTCCAGAGTTCAACACGGTTTCTACCTCGTCTTTTGGCGATATACAGAGCTTCATCgg                                                                    <  2:4932372/140‑1 (MQ=255)

                                                                    g‑AAAAAAATGCCATCGGCGGCATTATCCGCATCGGTTAGCTTCATGATTACCTCTGCATCCTGGCGCATCTAAAGACTGGCTTTCCAGAGTTCAACACGGTTTCTACCTCGTCTTTTGGCGATATACAGAGCTTCATCgg                                                                    <  1:648340/140‑1 (MQ=255)

                                                                    g‑AAAAAAATGCCATCGGCGGCATTATCCGCATCGGTTAGCTTCATGATTACCTCTGCATCCTGGCGCATCTAAAGACTGGCTTTCCAGAGTTCAACACGGTTTCTACCTCGTCTTTTGGCGATATACAGAGCTTCATCgg                                                                    <  1:1893382/140‑1 (MQ=255)

                                                                    g‑AAAAAAATGCCATCGGCGGCATTATCCGCATCGGTTAGCTTCATGATTACCTCTGCATCCTGGCGCATCTAAAGACTGGCTTTCCAGAGTTCAACACGGTTTCTACCTCGTCTTTTGGCGATATACAGAGCTTCATCgg                                                                    <  2:9016838/140‑1 (MQ=255)

                                                                    g‑AAAAAAATGCCATCGGCGGCATTATCCGCATCGGTTAGCTTCATGATTACCTCTGCATCCTGGCGCATCTAAAGACTGGCTTTCCAGAGTTCAACACGGTTTCTACCTCGTCTTTTGGCGATATACAGAGCTTCATCgg                                                                    <  1:7938865/140‑1 (MQ=255)

                                                                                 aTCGGCGGCATTATCCGCATCGGTTAGCTTCATGATTACCTCTGCATCCTGGCGCATCTAAAGACTGGCTTTCCAGAGTTCAACACGGTTTCTACCTCGTCTTTTGGCGATATACAGAGCTTCATCGGCTATTTGAATGa                                                        <  2:917176/140‑1 (MQ=255)

                                                                                   cggcggCATTATCCGCATCGGTTAGCTTCATGATTACCTCTGCATCCTGGCGCATCTAAAGACTGGCTTTCCAGAGTTCAACACGGTTTCTACCTCGTCTTTTGGCGATATACAGAGCTTCATCGGCTATTTGAATGAgg                                                      <  2:426654/140‑1 (MQ=255)

                                                                                   cggcggCATTATCCGCATCGGTTAGCTTCATGATTACCTCTGCATCCTGGCGCATCTAAAGACTGGCTTTCCAGAGTTCAACACGGTTTCTACCTCGTCTTTTGGCGATATACAGAGCTTCATCGGCTATTTGAATGAgg                                                      <  1:7495382/140‑1 (MQ=255)

                                                                                   cggcggCATTATCCGCATCGGTTAGCTTCATGATTACCTCTGCATCCTGGCGCATCTAAAGACTGGCTTTCCAGAGTTCAACACGGTTTCTACCTCGTCTTTTGGCGATATACAGAGCTTCATCGGCTATTTGAATGAgg                                                      <  2:4394083/140‑1 (MQ=255)

                                                                                   cggcggCATTATCCGCATCGGTTAGCTTCATGATTACCTCTGCATCCTGGCGCATCTAAAGACTGGCTTTCCAGAGTTCAACACGGTTTCTACCTCGTCTTTTGGCGATATACAGAGCTTCATCGGCTATTTGAATGAgg                                                      <  2:9072678/140‑1 (MQ=255)

                                                                                   cggcggCATTATCCGCATCGGTTAGCTTCATGATTACCTCTGCATCCTGGCGCATCTAAAGACTGGCTTTCCAGAGTTCAACACGGTTTCTACCTCGTCTTTTGGCGATATACAGAGCTTCATCGGCTATTTGAATGAgg                                                      <  1:1684663/140‑1 (MQ=255)

                                                                                     gcggcATTATCCGCATCGGTTAGCTTCATGATTACCTCTGCATCCTGGCGCATCTAAAGACTGGCTTTCCAGAGTTCAACACGGTTTCTACCTCGTCTTTTGGCGATATACAGAGCTTCATCGGCTATTTGAATGAGgcg                                                    >  1:2742434/1‑140 (MQ=255)

                                                                                       ggcATTATCCGCATCGGTTAGCTTCATGATTACCTCTGCATCCTGGCGCATCTAAAGACTGGCTTTCCAGAGTTCAACACGGTTTCTACCTCGTCTTTTGGCGATATACAGAGCTTCATCGGCTATTTGAATGAGGCGCt                                                  <  1:4746527/140‑1 (MQ=255)

                                                                                        gcATTATCCGCATCGGTTAGCTTCATGATTACCTCTGCATCCTGGCGCATCTAAAGACTGGCTTTCCAGAGTTCAACACGGTTTCTACCTCGTCTTTTGGCGATATACAGAGCTTCATCGGCTATTTGAATGAGGCGCTc                                                 >  1:8241833/1‑140 (MQ=255)

                                                                                         cATTATCCGCATCGGTTAGCTTCATGATTACCTCTGCATCCTGGCGCATCTAAAGACTGGCGTTCCAGAGTTCAACACGGTTTCTACCTCGTCTTTTGGCGATATACAGAGCTTCATCGGCTATTTGAATGAGGCGCTCa                                                <  2:6338449/140‑1 (MQ=255)

                                                                                             aTCCGCATCGGTTAGCTTCATGATTACCTCTGCATCCTGGCGCATCTAAAGACTGGCTTTCCAGAGTTCAACACGGTTTCTACCTCGTCTTTTGGCGATATACAGAGCTTCATCGGCTATTTGAATGAGGCGCTCATAGt                                            <  2:8403142/140‑1 (MQ=255)

                                                                                                  cATCGGTTAGCTTCATGATTACCTCTGCATCCTGGCGCATCTAAAGACTGGCTTTCCAGAGTTCAACACGGTTTCTACCTCGTCTTTTGGCGATATACAGAGCTTCATCGGCTATTTGAATGAGGCGCTCATAGTCAGGa                                       >  1:5206903/1‑140 (MQ=255)

                                                                                                   ctCGGTTAGCTTCATGATTACCTCTGCAGCCTGGCGCATCTAAAGACTGGCGTTCCATAGTTCAACACGGTTTCTACCTCGACTTTTGGCGATATACAGAGCTTCATCGGCTATTTGAAGGAGGCGCTCATAGTCAGGAt                                      <  2:1087899/139‑1 (MQ=255)

                                                                                                   agcGGTTAGCTTCATGATTACCTCTGCATCCTGGCGCATCTAAAGACTGGCTTTCCAGAGTTCAACACGGTTTCTACCTCGTCTTTTGGCGATATACAGAGCTTCATCGGCTATTTGAATGAGGCGCTCATAGTCAGGAt                                      >  2:6184721/3‑140 (MQ=255)

                                                                                                   aTCGGTTAGCTTCATGATTACCTCTTCATCCTGGCGCATCTCAAGACTGTATTTCCAGAGTTCAACACGGTTTCTACCTCGTCTTTTGGCGATATACAGATCTTAATCGGCTATTTGAATGAGGCGCTCATAGTCAGGAt                                      <  2:3021159/140‑1 (MQ=255)

                                                                                                   aTCGGTTAGCTTCATGATTACCTCTGCATCCTGGCGCATCTAAAGACTGGCTTTCCAGAGTTCAACACGGTTTCTACCTCGTCTTTTGGCGATATACAGAGCTTCATCGGCTATTTGACTGAGGCGCTCATAGTCAGGAt                                      >  2:8844380/1‑140 (MQ=255)

                                                                                                   aTCGGTTAGCTTCATGATTACCTCTGCATCCTGGCGCATCTAAAGACTGGCTTTCCAGAGTTCAACACGGTTTCTACCTCGTCTTTTGGCGATATACAGAGCTTCATCGGCTATTTGAATGAGGCGCTCATAGTCAGGAt                                      <  1:4174453/140‑1 (MQ=255)

                                                                                                   aTCGGTTAGCTTCATGATTACCTCTGCATCCTGGCGCATCTAAAGACTGGCTTTCCAGAGTTCAACACGGTTTCTACCTCGTCTTTTGGCGATATACAGAGCTTCATCGGCTATTTGAATGAGGCGCTCATAGTCAGGAt                                      <  1:2999353/140‑1 (MQ=255)

                                                                                                   aTCGGTTAGCTTCATGATTACCTCTGCATCCTGGCGCATCTAAAGACTGGCTTTCCAGAGTTCAACACGGTTTCTACCTCGTCTTTTGGCGATATACAGAGCTTCATCGGCTATTTGAATGAGGCGCTCATAGTCAGGAt                                      >  1:3140950/1‑140 (MQ=255)

                                                                                                   aTCGGTTAGCTTCATGATTACCTCTGCATCCTGGCGCATCTAAAGACTGGCTTTCCAGAGTTCAACACGGTTTCTACCTCGTCTTTTGGCGATATACAGAGCTTCATCGGCTATTTGAATGAGGCGCTCATAGTCAGGAt                                      >  2:1835288/1‑140 (MQ=255)

                                                                                                   aTCGGTTAGCTTCATGATTACCTCTGCATCCTGGCGCATCTAAAGACTGGCTTTCCAGAGTTCAACACGGTTTCTACCTCGTCTTTTGGCGATATACAGAGCTTCATCGGCTATTTGAATGAGGCGCTCATAGTCAGGAt                                      <  1:8078640/140‑1 (MQ=255)

                                                                                                    tCGGTGAGCTTCATGATTACCTCTGCATCCTGGCGCATCTAAAGACTGGCTTTCCAGAGTTCAACACGGTTTCTACCTCGTCTTTTGGCGATATACAGAGCTTCATCGGCTATTTGAATGAGGCGCTCATAGTCAGGATg                                     <  2:8120728/140‑1 (MQ=255)

                                                                                                        ttAGCTTCATGATTACCTCTGCATCCTGGCGCATCTAAAGACTGGCTTTCCAGAGTTCAACACGGTTTCTACCTCGTCTTTTGGCGATATACAGAGCTTCATCGGCTATTTGAATGAGGCGCTCATAGTCAGGATGACCa                                 <  2:5702909/140‑1 (MQ=255)

                                                                                                        ttAGCTTCATGATTACCTCTGCATCCTGGCGCATCTAAAGACTGGCTTTCCAGAGTTCAACACGGTTTCTACCTCGTCTTTTGGCGATATACAGAGCTTCATCGGCTATTTGAATGAGGCGCTCATAGTCAGGATGACCa                                 >  1:9060736/1‑140 (MQ=255)

                                                                                                        ttAGCTTCATGATTACCTCTGCATCCTGGCGCATCTAAAGACTGGCTTTCCAGAGTTCAACACGGTTTCTACCTCGTCTTTTGGCGATATACAGAGCTTCATCGGCTATTTGAATGAGGCGCTCATAGTCAGGATGACCa                                 >  1:5489430/1‑140 (MQ=255)

                                                                                                        ttAGCTTCATGATTACCTCTGCATCCTGGCGCATCTAAAGACTGGCTTTCCAGAGTTCAACACGGTTTCTACCTCGTCTTTTGGCGATATACAGAGCTTCATCGGCTATTTGAATGAGGCGCTCATAGTCAGGATGACCa                                 <  1:724266/140‑1 (MQ=255)

                                                                                                        ttAGCTTCATGATTACCTCTGCATCCTGGCGCATCTAAAGACTGGCTTTCCAGAGTTCAACACGGTTTCTACCTCGTCTTTTGGAGATATACAGAGCTTCATCGGCTATTTGAATGAGGCGCTCATAGTCAGGATGACCa                                 >  2:4078993/1‑140 (MQ=255)

                                                                                                           gCTTCATGATTACCTCTGCATCCTGGCGCATCTAAAGACTGGCTTTCCAGAGTTCAACACGGTTTCTACCTCGTCTTTTGGCGATATACAGAGCTTCATCGGCTATTTGAATGAGGCGCTCATAGTCAGGATGACCATTa                              <  1:6837903/140‑1 (MQ=255)

                                                                                                             ttCATGATTACCTCTGCATCCTGGCGCATCTAAAGACTGGCTTTCCAGAGTTCAACACGGTTTCTACCTCGTCTTTTGGCGATATACAGAGCTTCATCGGCTATTTGAATGAGGCGCTCATAGTCAGGATGACCATTaaa                            <  2:3627622/140‑1 (MQ=255)

                                                                                                             ttCATGATTACCTCTGCATCCTGGCGCATCTAAAGACTGGCTTTCCAGAGTTCAACACGGTTTCTACCTCGTCTTTTGGCGATATACAGAGCTTCATCGGCTATTTGAATGAGGCGCTCATAGTCAGGATGACCATTaaa                            <  1:2321873/140‑1 (MQ=255)

                                                                                                             ttCATGATTACCTCTGCATCCTGGCGCATCTAAAGACTGGCTTTCCAGAGTTCAACACGGTTTCTACCTCGTCTTTTGGCGATATACAGAGCTTCATCGGCTATTTGAATGAGGCGCTCATAGTCAGGATGACCATTaaa                            <  2:1650638/140‑1 (MQ=255)

                                                                                                             ttCATGATTACCTCTGCATCCTGGCGCATCTAAAGACTGGCTTTCCAGAGTTCAACACGGTTTCTACCTCGTCTTTTGGCGATATACAGAGCTTCATCGGCTATTTGAATGAGGCGCTCATAGTCAGGATGACCATTaaa                            <  1:1000685/140‑1 (MQ=255)

                                                                                                                 tGATTACCTCTGCATCCTGGCGCATCTAAAGACTGGCTTTCCAGAGTTCAACACGGTTTCTACCTCGTCTTTTGGCGATATACAGAGCTTCATCGGCTATTTGAATGAGGCGCTCATAGTCAGGATGACCATTAAACATg                        >  1:1195198/1‑140 (MQ=255)

                                                                                                                     tACCTCTGCATCCTGGCGCATCTAAAGACTGGCTTTCCAGAGTTCAACACGGTTTCTACCTCGTCTTTTGGCGATATACAGAGCTTCATCGGCTATTTGAATGAGGCGCTCATAGTCAGGATGACCATTAAACATggcgg                    <  1:5887257/140‑1 (MQ=255)

                                                                                                                      aCCTCTGCATCCTGGCGCATCTAAAGACTGGCTTTCCAGAGTTCAACACGGTTTCTACCTCGTCTTTTGGCGATATACAGAGCTTCATCGGCTATTTGACTGAGGCGCTCATAGTCAGGATGACCATTAAACATggcggc                   >  2:4681201/1‑140 (MQ=255)

                                                                                                                      aCCTCTGCATCCTGGCGCATCTAAAGACTGGCTTTCCAGAGTTCAACACGGTTTCTACCTCGTCTTTTGGCGATATACAGAGCTTCATCGGCTATTTGAATGAGGCGCTCATAGTCAGGATGACCATTAAACATggcggc                   >  2:3840861/1‑140 (MQ=255)

                                                                                                                       ccTCTGCATCCTGGCGCATCTAAAGACGGGCTGTCCAGAGTTCAACACGGTTTCTACCTCATCTTTTGGCGATATACAGAGCTTCATCGGCTATTTGAATGAGGCGCTCATAGTCAGGATGACCATTAAACATGGCGGCa                  <  1:6734485/140‑1 (MQ=255)

                                                                                                                          ctGCATCCTGGCGCATCTAAAGACTGGCTTTCCAGAGTTCAACACGGTTTCTACCTCGTCTTTTGGCGATATACAGAGCTTCATCGGCTATTTGAATGAGGCGCTCATAGTCAGGATGACCCTTAAACATGGCGGCACCg               >  2:2607862/1‑140 (MQ=255)

                                                                                                                           tGCATCCTGGCGCATCTAAAGACTGGCTTTCCAGAGTTCAACACGGTTTCTACCTCGTCTTTTGGCGATATACAGAGCTTCATCGGCTATTTGAATGAGGCGCTCATAGTCAGGATGACCATTAAACATGGCGGCACCGa              <  1:3932674/140‑1 (MQ=255)

                                                                                                                           tGCATCCTGGCGCATCTAAAGACTGGCTTTCCAGAGTTCAACACGGTTTCTACCTCGTCTTTTGGCGATATACAGAGCTTCATCGGCTATTTGAATGAGGCGCTCATAGTCAGGATGACCATTAAACATGGCGGCACCGa              <  1:8890787/140‑1 (MQ=255)

                                                                                                                               tCCTGGCGCATCTAAAGACTGGCTTTCCAGAGTTCAACACGGTTTCTACCTCGTCTTTTGGCGATATACAGAGCTTCATCGGCTATTTGAATGAGGCGCTCATAGTCAGGATGTCCATTAAACATGGCGGCACCGATGGa          <  1:1309718/140‑1 (MQ=255)

                                                                                                                               tCCTGGCGCATCTAAAGACTGGCTTTCCAGAGTTCAACACGGTTTCTACCTCGTCTTTTGGCGATATACAGAGCTTCATCGGCTATTTGAATGAGGCGCTCATAGTCAGGATGACCATTAAACATGGCGGCACCGATGGa          >  2:7259951/1‑140 (MQ=255)

                                                                                                                                  tGGCGCATCTAAAGACTGGCTTTCCAGAGTTCAACACGGTTTCTACCTCGTCTTTTGGCGATATACAGAGCTTCATCGGCTATTTGAATGAGGCGCTCATAGTCAGGATGACCATTAAACATGGCGGCACCGATGGAAAg       >  2:6683289/1‑140 (MQ=255)

                                                                                                                                  tGGCGCATCTAAAGACTGGCTTTCCAGAGTTCAACACGGTTTCTACCTCGTCTTTTGGCGATATACAGAGCTTCATCGGCTATTTGAATGAGGCGCTCATAGTCAGGATGACCATTAAACATGGCGGCACCGATGGAAAg       >  1:7429270/1‑140 (MQ=255)

                                                                                                                                  tGGCGCATCTAAAGACTGGCTTTCCAGAGTTCAACACGGTTTCTACCTCGTCTTTTGGCGATATACAGAGCTTCATCGGCTATTTGAATGAGGCGCTCATAGTCAGGATGACCATTAAACATGGCGGCACCGATGGAAAg       >  2:5372435/1‑140 (MQ=255)

                                                                                                                                   ggCGCATCTAAAGACTGGCTTTCCAGAGTTCAACTCGGTTTCTCCCTCGTCTTTTGGCGATATAGAGAGCGTCATCGGCTATTTGAATGAGGCGCTCATAGTGAGGTTGTCCATTAAACATGGCGGAACCGTTGGAAAGt      >  2:1933843/1‑140 (MQ=255)

                                                                                                                                   ggCGCATCTAAAGACTGGCTTTCCAGAGTTCAACACGGTTTCTACCTCGTCTTTTGGCGATATACAGAGCTTCATCGGCTATTTGAATGAGGCGCTCATAGTCAGGATGACCATTAAACATGGCGGCACCGATGGAAAGt      <  1:6078260/140‑1 (MQ=255)

                                                                                                                                   ggCGCATCTAAAGACTGGCTTTCCAGAGTTCAACACGGTTTCTACCTCGTCTTTTGGCGATATACAGAGCTTCATCGGCTATTTGAATGAGGCGCTCATAGTCAGGATGACCATTAAACATGGCGGCACCGATGGAAAGt      >  1:9177988/1‑140 (MQ=255)

                                                                                                                                   ggCGCATCTAAAGACTGGCTTTCCAGAGTTCAACACGGTTTCTACCTCGTCTTTTGGCGATATACAGAGCTTCATCGGCTATTTGAATGAGGCGCTCATAGTCAGGATGACCATTAAACATGGCGGCACCGATGGAAAGt      >  1:4525080/1‑140 (MQ=255)

                                                                                                                                    gcgcATCTAAAGACTGGCTTTCCAGAGTTCAACACGGTTTCTACCTCGTCTTTTGGCGATATACAGAGCTTCATCGGCTATTTGAATGAGGCGCTCATAGTCAGGATGACCATTAAACATGGCGGCACCGATGGAAAGTg     >  2:2360874/1‑140 (MQ=255)

                                                                                                                                      gcATCTAAAGACTGGCTTTCCAGAGTTCAACACGGTTTCTACCTCGTCTTTTGGCGATATACAGAGCTTCATCGGCTATTTGAATGAGGCGCTCATAGTCAGGATGACCATTAAACATGGCGGCACCGATGGAAAGTgag   <  2:3268930/140‑1 (MQ=255)

                                                                                                                                       cATCTAAAGACTGGCTTTCCAGAGTTCAACACGGTTTCTACCTCGTCTTTTGGCGATATACAGAGCTTCATCGGCTCTTTGAATGAGGCGCTCATAGTCAGGATGACCATTAAACATGGCGGCACCGATGGAAAGTgaga  >  1:4856844/1‑140 (MQ=255)

                                                                                                                                       cATCTAAAGACTGGCTTTCCAGAGTTCAACACGGTTTCTACCTCGTCTTTTGGCGATATACAGAGCTTCATCGGCTATTTGAATGAGGCGCTCATAGTCAGGATGACCATTAAACATGGCGGCACCGATGGAAAGTgaga  >  1:4314354/1‑140 (MQ=255)

TTGAAAAACATCACTTCATCATTTTCGTTAATTAACACCGCACCCATCATATTTTGCTCAAGGGCGGGG‑AAAAAAATGCCATCGGCGGCATTATCCGCATCGGTTAGCTTCATGATTACCTCTGCATCCTGGCGCATTTAAAGACTGGCTTTCCAGAGTTCAACACGGTTTCTACCTCGTCTTTTGGCGATATACAGAGCTTCATCGGCTATTTGAATGAGGCGCTCATAGTCAGGATGACCATTAAACATGGCGGCACCGATGGAAAGTGAGA  >  NZ_CP025268/1570440‑1570713

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`