BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP025268	160,613	T→C	235 (TAA→TAG)	sfsA ←	DNA/RNA nuclease SfsA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP025268	160,613	0	T	C	99.8%	1795.9 / NA	513	235 (TAA→TAG)	sfsA	DNA/RNA nuclease SfsA
Reads supporting (aligned to +/- strand): ref base T (0/0); major base C (276/236); minor base G (0/1); total (276/237)
Fisher's exact test for biased strand distribution p-value = 4.62e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 537 total aligned reads displayed.

GGGGAAAAATGAGGCCGCTATAAATAGCAGATGCTTTTCCGGATAGCAATTATCTAAACGTAACACTTGACAACTGTGTGAGGAAAAGCGTATTTGCGCACGCGACCAGAATGTAAATTAACCAGTTACTTACTTTATTACAATGTAACCGGCAGTGATTTTTTAAGAGCCATGCCTTCAGCAGAAATTTCCGCTTTGTAAGCCAGAATTTCTACCCCCCTCTGTTGAGCTTCTGACAATAGTTGCGCGTATTTCTCATCGATGTGGCGCGC  >  NZ_CP025268/160476‑160747

ggggAAAAATGAGGCCGCTATAAATAGCAGATGCTTTTCCGGATAGCAATTATCTAAACGTAACACTTGACAACTGTGTGAGGAAAAGCGTATTTGCGCACGCGACCAGAATGTAAATTAACCAGTTACTTACTttacta                                                                                                                                      <  1:5704350/140‑1 (MQ=255)

ggggAAAAATGAGGCCGCTATAAATAGCAGATGCTTTTCCGGATAGCAATTATCTAAACGTAACACTTGACAACTGTGTGAGGAAAAGCGTATTTGCGCACGCGACCAGAATGTAAATTAACCAGTTACTTACTttacta                                                                                                                                      <  2:4054510/140‑1 (MQ=255)

ggggAAAAATGAGGCCGCTATAAATAGCAGATGCTTTTCCGGATAGCAATTATCTAAACGTAACACTTGACAACTGTGTGAGGAAAAGCGTATTTGCGCACGCGACCAGAATGTAAATTAACCAGTTACTTACTttacta                                                                                                                                      <  1:7896810/140‑1 (MQ=255)

ggggAAAAATGAGGCCGCTATAAATAGCAGATGCTTTTCCGGATAGCAATTATCTAAACGTAACACTTGACAACTGTGTGAGGAAAAGCGTATTTGCGCACGCGACCAGAATGTAAATTAACCAGTTACTTACTttacta                                                                                                                                      <  1:3667347/140‑1 (MQ=255)

  ggAAAAATGAGGCCGCTATAAATAGCAGATGCTTTTCCGGATAGCAATTATCTAAACGTAACACTTGACAACTGTGTGAGGAAAAGCGTATTTGCGCACGCGACCAGAATGTAAATTAACCAGTTACTTACTTTACTACa                                                                                                                                    <  2:2949408/140‑1 (MQ=255)

  ggAAAAATGAGGCCGCTATAAATAGCAGATGCTTTTCCGGATAGCAATTATCTAAACGTAACACTTGACAACTGTGTGAGGAAAAGCGTATTTGCGCACGCGACCAGAATGTAAATTAACCAGTTACTTACTTTACTACa                                                                                                                                    <  2:1019058/140‑1 (MQ=255)

    aaaaaTGAGGCCGCTATAAATAGCAGATGCTTTTCCGGATAGCAATTATCTAAACGTAACACTTGACAACTGTGTGAGGAAAAGCGTATTTGCGCACGCGACCAGAATGTAAATTAACCAGTTACTTACTTTACTACAAt                                                                                                                                  <  2:9054677/140‑1 (MQ=255)

       aaTGAGGCCGCTATAAATAGCAGATGCTTTTCCGGATAGCAATTATCTAAACGTAACACTTGACAACTGTGTGAGGAAAAGCGTATTTGCGCACGCGACCAGAATGTAAATTAACCAGTTACTTACTTTACTACAATGTa                                                                                                                               <  1:6415687/140‑1 (MQ=255)

         tGAGGCCGCTATAAATAGCAGATGCTTTTCCGGATAGCAATTATCTAAACGTAACACTTGACAACTGTGTGAGGAAAAGCGTATTTGCGCACGCGACCAGAATGTAAATTAACCAGTTACTTACTTTACTACAATGTAAc                                                                                                                             >  1:9372054/1‑140 (MQ=255)

                 cTATAAATAGCAGATGCTTTTCCGGATAGCAATTATCTAAACGTAACACTTGACAACTGTGTGAGGAAAAGCGTATTTGCGCACGCGACCAGAATGTAAATTAACCAGTTACTTACTTTACTACAATGTAACCGGCAGTg                                                                                                                     >  1:5379011/1‑140 (MQ=255)

                 cTATAAATAGCAGATGCTTTTCCGGATAGCAATTATCTAAACGTAACACTTGACAACTGTGTGAGGAAAAGCGTATTTGCGCACGCGACCAGAATGTAAATTAACCAGTTACTAACTTTACTACAATGTAACCGGCAGTg                                                                                                                     >  1:3885562/1‑140 (MQ=255)

                         aGCAGATGCTTTTCCGGATAGCAATTATCTAAACGTAACACTTGACAACTGTGTGAGGAAAAGCGTATTTGCGCACGCGACCAGAATGTAAATTAACCAGTTACTTACTTTACTACAATGTAACCGGCAGTGATTTTTTa                                                                                                             >  2:2991406/1‑140 (MQ=255)

                           cAGATGCTTTTCCGGATAGCCATTATCTAAACGTAACACTTGACAACTGTGTGAGGCAAAGCGTATTTGCGCACGCGACCAGAATGTCAATTAACCAGTTACTTACTTTACTCCAATGTAACCGGCAGTGATTTTTTAag                                                                                                           >  2:1160455/1‑140 (MQ=255)

                           cAGATGCTTTTCCGGATAGCAATTATCTAAACGTAACACTTGACAACTGTGTGAGGAAAAGCGTATTTGCGCACGCGACCAGAATGTAAATTAACCAGTTACTTACTTTACTACAATGTAACCGGCAGTGATTTTTTAag                                                                                                           >  1:2683139/1‑140 (MQ=255)

                           cAGATGCTTTTCCGGATAGCAATTATCTAAACGTAACACTTGACAACTGTGTGAGGAAAAGCGTATTTGCGCACGCGACCAGAATGTAAATTAACCAGTTACTTACTTTACTACAATGTAACCGGCAGTGATTTTTTAag                                                                                                           >  1:8714309/1‑140 (MQ=255)

                           cAGATGCTTTTCCGGATAGCAATTATCTAAACGTAACACTTGACAACTGTGTGAGGAAAAGCGTATTTGCGCACGCGACCAGAATGTAAATTAACCAGTTACTTACTTTACTACAATGTAACCGGCAGTGATTTTTTAag                                                                                                           >  2:9303110/1‑140 (MQ=255)

                            aGATGCTTTTCCGGATAGCAATTATCTAAACGTAACACTTGACAACTGTGTGAGGAAAAGCGTATTTGCGCACGCGACCAGAATGTAAATTAACCAGTTACTTACTTTACTGCAATGTAACCGGCAGTGATTTTTTAtga                                                                                                          >  1:8367068/1‑140 (MQ=255)

                              aTGCTTTTCCGTATAGCAATTATCTAAACGTAACACTTGACAACTGTGTGAGGAAAAGCGTATTTGCGCACGCGACCAGAATGTAAATTAACCAGTTACTTACTTTACTACAATGTAACCGGCAGTGATTTTTTAAGAGc                                                                                                        >  1:171696/1‑140 (MQ=255)

                              aTGCTTTTCCGGATAGCAATTATCTAAACGTAACACTTGACAACTGTGTGAGGAAAAGCGTATTTGCGCACGCGACCAGAATGTAAATTAACCAGTTACTTACTTTACTACAATGTAACCGGCAGt                                                                                                                      <  2:2133239/126‑1 (MQ=255)

                              aTGCTTTTCCGGATAGCAATTATCTAAACGTAACACTTGACAACTGTGTGAGGAAAAGCGTATTTGCGCACGCGACCAGAATGTAAATTAACCAGTTACTTACTTTACTACAATGTAACCGGCAGTGATTTTTTATGAGc                                                                                                        >  2:6295915/1‑140 (MQ=255)

                              aTGCTTTTCCGGATAGCAATTATCTAAACGTAACACTTGACAACTGTGTGAGGAAAAGCGTATTTGCGCACGCGACCAGAATGTAAATTAACCAGTTACTTACTTTACTACAATGTAACCGGCAGTGATTTTTTAAGAGc                                                                                                        >  1:1135684/1‑140 (MQ=255)

                              aTGCTTTTCCGGATAGCAATTATCTAAACGTAACACTTGACAACTGTGTGAGGAAAAGCGTATTTGCGCACGCGACCAGAATGTAAATTAACCAGTTACTTACTTTACTACAATGTAACCGGCAGTGATTTTTTAAGAGc                                                                                                        >  2:3082612/1‑140 (MQ=255)

                               tGCTTTTCCGGATAGCAATTATCTAAACGTAACACTTGACAACTGTGTGAGGAAAAGCGTATTTGCGCACGCGACCAGAATGTAAATTAACCAGTTACTTACTTTACTACAATGTAACCGGCAGTGATTTTTTAAGAGcc                                                                                                       <  1:751671/140‑1 (MQ=255)

                                gCTTTTCCGGATAGCAATTATCTAAACGTAACACTTGACAACTGTGTGAGGAAAAGCGTATTTGCGCACGCGACCAGAATGTAAATTAACCAGTTACTTACTTTACTACAATGTAACCGGCAGTGATTTTTTAAGAGCCa                                                                                                      >  1:3958778/1‑140 (MQ=255)

                                    ttCCGGATAGCAATTATCTACACGTAACACTTGACAACTGTGTGAGGAAAAGCGTATTTGCGCACGCGACCAGAATGTAAATTAACCAGTTACTTACTTTACTACAATGTAACCGGCAGTGATTTTTTAAGAGCCATGcc                                                                                                  <  1:3592423/140‑1 (MQ=255)

                                    ttCCGGATAGCAATTATCTAAACGTAACACTTGACAACTGTGTGAGGAAAAGCGTATTTGCGCACGCGACCAGAATGTAAATTAACCAGTTACTTACTTTACTACAATGTAACCGGCAGTGATTTTTTAAGAGCCATGcc                                                                                                  <  2:243093/140‑1 (MQ=255)

                                    ttCCGGATAGCAATTATCTAAACGTAACACTTGACAACTGTGTGAGGAAAAGCGTATTTGCGCACGCGACCAGAATGTAAATTAACCAGTTACTTACTTTACTACAATGTAACCGGCAGTGATTTTTTAAGAGCCATGcc                                                                                                  >  1:7101965/1‑140 (MQ=255)

                                              cAATTATCTAAACGTAACACTTGACAACTGTGTGAGGAAAAGCGTATTTGCGCACGCGACCAGAATGTAAATTAACCAGTTACTTACTTTACTACAATGTAACCGGCAGTGATTTTTTAAGAGCCATGCCTTCAGCAGaa                                                                                        >  2:7692180/1‑140 (MQ=255)

                                                   aTCTAAACGTAACACTTGACAACTGTGTGAGGAAAAGCGTATTTGCGCACGCGACCAGAATGTAAATTAACCAGTTACTTACTTTACTACAATGTAACCGGCAGTGATTTTTTAAGAGCCATGCCTTCAGCAGAAATTTc                                                                                   >  1:1815146/1‑140 (MQ=255)

                                                     cTAAACGTAACACTTGACAACTGTGTGAGGAAAAGCGTATTTGCGCACGCGACCAGAATGTAAATTAACCAGTTACTTACTTTACTACAATGTAACCGGCAGTGATTTTTTAAGAGCCATGCCTTCAGCAGAAATTTCCg                                                                                 <  2:5972618/140‑1 (MQ=255)

                                                     cTAAACGTAACACTTGACAACTGTGTGAGGAAAAGCGTATTTGCGCACGCGACCAGAATGTAAATTAACCAGTTACTTACTTTACTACAATGTAACCGGCAGTGATTTTTTAAGAGCCATGCCTTCAGCAGAAATTTCCg                                                                                 <  1:8193796/140‑1 (MQ=255)

                                                        aaCGTAACACTTGACAACTGTGTGAGGAAAAGCGTATTTGCGCACGCGACCAGAATGTAAATTAACCAGTTACTTACTTTACTACAATGTAACCGGCAGTGATTTTTTAAGAGCCATGCCTTCAGCAGAAATTTCCGCtt                                                                              >  2:6835277/1‑140 (MQ=255)

                                                        aaCGTAACACTTGACAACTGTGTGAGGAAAAGCGTATTTGCGCACGCGACCAGAATGTAAATTAACCAGTTACTTACTTTACTACAATGTAACCGGCAGTGATTTTTTAAGAGCCATGCCTTCAGCAGAAATTTCCGCtt                                                                              >  1:1618315/1‑140 (MQ=255)

                                                        aaCGTAACACTTGACAACTGTGGGAGGAAAAGCGTATTTGCGCACGCGACCAGAATGTAAATTAACCAGTTACTTACTTTACTACAATGTAACAGGCAGTGATTTTTTATGAGCCATGCCTTCAGCAGACATTTACGCtt                                                                              >  2:6520234/1‑140 (MQ=255)

                                                        aaCGTAACACATGACAACGGAGTGAGGAAAAGCGTATTTCCGCACGCGACCAGAATGTAAAAAAACCAGTTACTTACTTAACTACAATGTAACCGGCAGTGATTTTTTAAGAGCCATGCCGTCAGCAGAAATTTCCGcat                                                                              >  2:4216809/1‑138 (MQ=255)

                                                          cGTAACACTTGACAACTGTGTGAGGAAAAGCGTATTTGCGCACGCGACCAGAATGTAAATTAACCAGTTACTTACTTTACTACAATGTAACCGGCAGTGATTTTTTAAGAGCCATGCCTTCAGCAGAAATTTCCGCTTTg                                                                            <  1:8027907/140‑1 (MQ=255)

                                                           gTAACACTTGACAACTGTGTGAGGAAAAGCGTATTTGCGCACGCGACCAGAATGTAAATTAACCAGTTACTTACTTTACTACAATGTAACCGGCAGTGATTTTTTAAGAGCCATGCCTTCAGCAGAAATTTCCGCTTTGt                                                                           <  1:2079241/140‑1 (MQ=255)

                                                            tAACACTTGACAACTGTGTGAGGAAAAGCGTATTTGCGCACGCGACCAGAATGTAAATTAACCAGTTACTTACTTTACTACAATGTAACCGGCAGTGATTTTTTAAGAGCCATGCCTTCAGCAGAAATTTCCGCTTTGTa                                                                          <  2:8319486/140‑1 (MQ=255)

                                                             aaCACTTGACAACTGTGTGAGGAAAAGCGTATTTGCGCACGCGACCAGAATGTAAATTAACCAGTTACTTAATTTACTACAATGTAACCGGCAGTGATTTTTTAAGAGCCATGCCTTCAGCAGAAATTTCCGCTTTGTaa                                                                         >  2:8104820/1‑140 (MQ=255)

                                                              acacTTGACAACTGTGTGAGGAAAAGCGTATTTGCGCACGCGACCAGAATGTAAATTAACCAGTTACTTACTTTACTACAATGTAACCGGCAGTGATTTTTTAAGAGCCATGCCTTCAGCAGAAATTTCCGCTTTGTAAg                                                                        >  2:51825/1‑140 (MQ=255)

                                                                acTTGACAACTGTGTGAGGAAAAGCGTATTTGCGCACGCGACCAGAATGTAAATTAACCAGTTACTTACTTTACTACAATGTAACCGGCAGTGATTTTTTAAGAGCCATGCCTTCAGCAGAAATTTCCGCTTTGTAAGcc                                                                      <  1:1425949/140‑1 (MQ=255)

                                                                acTTGACAACTGGTTGAGGATAAGCGTATTTGCGCACGCGACCAGAATGTAAATTAACCAGGTACTTACTTTACTACCATGTAACCGGCAGTGATTTTTTAAGAGCCAGGCCTTCAGCAGAAATTTCCGCTTTGTACGcc                                                                      <  2:3285474/140‑1 (MQ=255)

                                                                 cTTGACAACTGTGTGAGGAAAAGCGTATTTGCGCACGCGACCAGAATGTAAATTAACCAGTTACTTACTTTACTACAATGTAACCGGCAGTGATTTTTTAAGAGCCATGCCTTCAGCAGAAATTTCCGCTTTGTAAGCCa                                                                     <  1:4439676/140‑1 (MQ=255)

                                                                    gACAACTGTGTGAGGAAAAGCGTATTTGCGCACGCGACCAGAATGTAAATTAACCAGTTACTTACTTTACTACAATGTAACCGGCAGTGATTTTTTAAGAGCCATGCCTTCAGCAGAAATTTCCGCTTTGTAAGCCAGat                                                                  >  2:8825976/1‑139 (MQ=255)

                                                                     aCAACTGTGTGAGGAAAAGCGTATTTGCGCACGCGACCAGAATGTAAATTAACCAGTTACTTACTTTACTACAATGTAACCGGCAGTGATTTTTTAAGAGCCATGCCTTCAGCAGAAATTTCCGCTTTGTAAGCCAGAAt                                                                 <  2:9160687/140‑1 (MQ=255)

                                                                       aaCTGTGTGAGGAAAAGCGTATTTGCGCACGCGACCAGAATGTAAATTAACCAGTTACTTACTTTACTACAATGTAACCGGCAGTGATTTTTTAAGAGCCATGCCTTCAGCAGAAATTTCCGCTTTGTAAGCCAGAAttt                                                               <  1:7632929/140‑1 (MQ=255)

                                                                       aaCTGTGTGAGGAAAAGCGTATTTGCGCACGCGACCAGAATGTAAATTAACCAGTTACTTACTTTACTACAATGTAACCGGCAGTGATTTTTTAAGAGCCATGCCTTCAGCAGAAATTTCCGCTTTGTAAGCCAGAAttt                                                               <  1:1213875/140‑1 (MQ=255)

                                                                         cTGTGTGAGGAAAAGCTTATTTGCGCACGCGACCAGAATGTAAATTAACCAGTTACTTACTTTACTACAATGTAACCGGCAGTGATTTTTTAAGAGCCATGCCTTCAGCAGAAATTTCCGCTTTGTAAGCCAGAATATCt                                                             >  2:2577664/1‑140 (MQ=255)

                                                                         cTGTGTGAGGAAAAGCGTATTTGCGCACGCGACCAGAATGTAAATTAACCAGTTACTTACTTTACTACAATGTAACCGGCAGTGATTTTTTAAGAGCCATGCCTTCAGCAGAAATTTCCGCTTTGTAAGACAGAATTTCt                                                             >  2:8680456/1‑140 (MQ=255)

                                                                          tgtgtgAGGAAAAGCGTATTTGCGCACGCGACCAGAATGTAAATTAACCAGTTACTTACTTTACTACAATGTAACCGGCAGTGATTTTTTAAGAGCCATGCCTTCAGCAGAAATTTCCGCTTTGTAAGCCAGAATTTCTa                                                            <  1:343094/140‑1 (MQ=255)

                                                                            tgtgAGGAAAAGCGTATTTGCGCACGCGACCAGAATGTAAATTAACCAGTTACTTACTTTACTACAATGTAACCGGCAGTGATTTTTTAAGAGCCATGCCTTCAGCAGAAATTTCCGCTTTGTAAGCCAGAATTTCTAcc                                                          >  1:2203847/1‑140 (MQ=255)

                                                                            tgtgAGGAAAAGCGTATTTGCGCACGCGACCAGAATGTAAATTAACCAGTTACTTACTTTACTACAATGTAACCGGCAGTGATTTTTTAAGAGCCATGCCTTCAGCAGAAATTTCCGCTTTGTAAGCCAGAATTTCTAcc                                                          <  2:160765/140‑1 (MQ=255)

                                                                            tgtgAGGAAAAGCGTATTTGCGCACGCGACCAGAATGTAAATTAACCAGTTACTTACTTTACTACAATGTAACCGGCAGTGATTTTTTAAGAGCCATGCCTTCAGCAGAAATTTCCGCTTTGTAAGCCAGAATTTCTAcc                                                          >  1:9087930/1‑140 (MQ=255)

                                                                            tgtgAGGAAAAGCGTATTTGCGCACGCGACCAGAATGTAAATTAACCAGTTACTTACTTTACTACAATGTAACCGGCAGTGATTTTTTAAGAGCCATGCCTTCAGCAGAAATTTCCGCTTTGTAAGCCAGAATTTCTAcc                                                          >  1:1011483/1‑140 (MQ=255)

                                                                            tgtgAGGAAAAGCGTATTTGCGCACGCGACCAGAATGTAAATTAACCAGTTACTTACTTTACTACAATGTAACCGGCAGTGATTTTTTAAGAGCCATGCCTTCAGCAGAAATTTCCGCTTTGTAAGCCAGAATTTCTAcc                                                          >  1:4897678/1‑140 (MQ=255)

                                                                            tgtgAGGAAAAGCGTATTTGCGCACGCGACCAGAATGTAAATTAACCAGTTACTTACTTTACTACAATGTAACCGGCAGTGATTTTTTAAGAGCCATGCCTTCAGCAGAAATTTCCGCTTTGTAAGCCAGAATTTCTAcc                                                          >  1:6928008/1‑140 (MQ=255)

                                                                               caGGAAAAGCGTAATTGCGCAAGCGACCAGAAAGTAAAAGAACCAGTTACTTACTTTACTCCAATGAAACCGGCAGTGATGTTTTAAGAGCCATGCCTTCAGCAGAAATTTCCGCTTTGTAAGCCAGAATTTCTAccccc                                                       <  2:8492076/139‑1 (MQ=255)

                                                                                  gAAAAGCGTATTTGCGCACGCGACCAGAATGTAAATTAACCAGTTACTTACTTTACTACAATGTAACCGGCAGTGATTTTTTAAGAGCCATGCCTTCAGCAGAAATTTCCGCTTTGTAAGCCAGAATTTCTACCCCCctc                                                    <  1:1971884/140‑1 (MQ=255)

                                                                                  gAAAAGCGTATTTGCGCACGCGACCAGAATGTAAATTAACCAGTTACTTACTTTACTACAATGTAACCGGCAGTGATTTTTTAAGAGCCATGCCTTCAGCAGAAATTTCCGCTTTGTAAGCCAGAATTTCTACCCCCctc                                                    <  1:6798473/140‑1 (MQ=255)

                                                                                  gAAAAGCGTATTTGCGCACGCGACCAGAATGTAAATTAACCAGTTACTTACTTTACTACAATGTAACCGGCAGTGATTTTTTAAGAGCCATGCCTTCAGCAGAAATTTCCGCTTTGTAAGCCAGAATTTCTACCCCCctc                                                    <  2:2717138/140‑1 (MQ=255)

                                                                                      aGCGTATTTGCGCACGCGTCCAGAATGTAAATTAACCAGTTACTTACTTTACTACAATGTAACCGGCAGTGATTTTTTAAGAGCCATGCCTTCAGCAGAAATTTCCGCTTTGTAAGCCAGAATTTCTACCCCCCTCTGtt                                                >  2:746659/1‑140 (MQ=255)

                                                                                      aGCGTATTTGCGCACGCGACCAGAATGTAAATTAACCAGTTACTTACTTTACTACAATGTAACCGGCAGTGATTTTTTAAGAGCCATGCCTTCAGCAGAAATTTCCGCTTTGTAAGCCAGAATTTCTa                                                            <  2:4989765/128‑1 (MQ=255)

                                                                                      aGCGTATTTGCGCACGCGACCAGAATGTAAATTAACCAGTTACTTACTTTACTACAATGTAACCGGCAGTGATTTTTTAAGAGCCATGCCTTCAGCAGAAATTTCCGCTTTGTAAGCCAGAATTTCTa                                                            >  1:4989765/1‑128 (MQ=255)

                                                                                      aGCGTATTTGCGCACGCGACCAGAATGTAAATTAACCAGTTACTTACTTTACTACAATGTAACCGGCAGTGATTTTTTAAGAGCCATGCCTTCAGCAGAAATTTCCGCTTTGTAAGCCAGAATTTCTACCCCCCTCTGtt                                                >  2:2084764/1‑140 (MQ=255)

                                                                                      aGCGTATTTGCGCACGCGACCAGAATGTAAATTAACCAGTTACTTACTTTACTACAATGTAACCGGCAGTGATTTTTTAAGAGCCATGCCTTCAGCAGAAATTTCCGCTTTGTAAGCCAGAATTTCTACCCCCCTCTGtt                                                >  2:2013183/1‑140 (MQ=255)

                                                                                      aGCGTATTTGCGCACGCGACCAGAATGTAAATTAACCAGTTACTTACTTTACTACAATGTAACCGGCAGTGATTTTTTAAGAGCCATGCCTTCAGCAGAAATTTCCGCTTTGTAAGCCAGAATTTCTACCCCCCTCTGtt                                                >  1:5839298/1‑140 (MQ=255)

                                                                                        cGTATTTGCGCACGCGACCAGAATGTAAATTAACCAGTTACTTACTTTACTACAATGTAACCGGCAGTGATTTTTTAAGAGCCATGCCTTCAGCAGAAATTTCCGCTTTGTAAGCCAGAATTTCTACCCCCCTCTGTTGa                                              >  2:7004920/1‑140 (MQ=255)

                                                                                          tATTTGCGCACGCGACCAGAATGTAAATTAACCAGTTACTTACTTTACTACAATGTAACCGGCAGTGATTTTTTAAGAGCCATGCCTTCAGCAGAAATTTCCGCTTTGTAAGCCAGAATTTCTACCCCCCTCTGTTGAGc                                            >  1:4103392/1‑140 (MQ=255)

                                                                                           aTTTGCGCACGCGACCAGAATGTAAATTAACCAGTTACTTACTTTTCTACAATGTAACCGGCAGTGTTTTTTTAAGAGCCATGCCTTCAGCAGATATTTCCGCTTTGTAAGCCTGAATTTCTACGCCGCTCTGTTGAGCt                                           <  1:3172764/140‑1 (MQ=255)

                                                                                           aTTTGCGCACGCGACCAGAATGTAAATTAACCAGTTACTTACTTTACTACAATGTAACCGGCAGTGATTTTTTAAGAGCCATGCCTTCAGCAGAAATTTCCGCTTTGTAAGCCAGAATTTCTACCCCCCTCTGTTGAGCt                                           <  1:5537467/140‑1 (MQ=255)

                                                                                            tttGCGCACGCGACCAGAATGTAAATTAACCAGTTACTTACTTTACTACAATGTAACCGGCAGTGATTTTTTAAGAGCCATGCCTTCAGCAGAAATTTCCGCTTTGTAAGCCAGAATTTCTACCCCCCTCTGTTGAGCtt                                          <  1:8887087/140‑1 (MQ=255)

                                                                                              tGCGCACGCGACCAGAATGTAAATTAACCAGTTACTTACTTTACTACAATGTAACCGGCAGTGATTTTTTAAGAGCCATGCCTTCAGCAGAAATTTCCGCTTTGTAAGCCAGAATTTCTACCCCCCTCTGTTGAGCTTCt                                        <  2:6690011/140‑1 (MQ=255)

                                                                                              tGCGCACGCGACCAGAATGTAAATTAACCAGTTACTTACTTTACTACAATGTAACCGGCAGTGATTTTTTAAGAGCCATGCCTTCAGCAGAAATTTCCGCTTTGTAAGCCAGAATTTCTACCCCCCTCTGTTGAGCTTCt                                        <  2:6074944/140‑1 (MQ=255)

                                                                                                  cACGCGACCAGAATGTAAATTAACCAGTTACTTACTTTACTACAATGTAACCGGCAGTGATTTTTTAAGAGCCATGCCTTCAGCAGAAATTTCCGCTTTGTAAGCCAGAATTTCTACCCCCCTCTGTTGAGCTTCTGACa                                    >  1:4296989/1‑140 (MQ=255)

                                                                                                  cACGCGACCAGAATGTAAATTAACCAGTTACTTACTTTACTACAATGTAACCGGCAGTGATTTTTTAAGAGCCATGCCTTCAGCAGAAATTTCCGCTTTGTAAGCCAGAATATCTACCCCCCTCTGTTGAGCTTCTGACa                                    >  2:3747825/1‑140 (MQ=255)

                                                                                                               tGTAAATTAACCAGTTACTTACTTTACTACAATGTAACCGGCAGTGATTTTTTAAGAGCCATGCCTTCAGCAGAAATTTCCGCTTTGTAAGCCAGAATTTCTACCCCCCTCTGTTGAGCTTCTGACAATAGTTGCGCGTa                       >  1:7377849/1‑140 (MQ=255)

                                                                                                                 tAAATTAACCAGTTACTTACTTTACTACAATGTAACCGGCAGTGATTTTTTAAGAGCCATGCCTTCAGCAGAAATTTCCGCTTTGTAAGCCAGAATTTCTACCCCCCTCTGTTGAGCTTCTGACACTAGTTGCGCGTAtt                     >  2:7178249/1‑140 (MQ=255)

                                                                                                                 tAAATTAACCAGTTACTTACTTTACTACAATGTAACCGGCAGTGATTTTTTAAGAGCCATGCCTTCAGCAGAAATTTCCGCTTTGTAAGCCAGAATTTCTACCCCCCTCTGTTGAGCTTCTGACAATAGTTGCGCGTAtt                     >  1:6021785/1‑140 (MQ=255)

                                                                                                                    aTTAACCAGTTACTTACTTTACTACAATGTAACCGGCAGTGATTTTTTAAGAGCCATGCCTTCAGCAGAAATTTCCGCTTTGTAAGCCAGAATTTCTACCCCCCTCTGTTTAGCTTCTGACAATAGTTGCGCGTATTtct                  <  2:1407096/140‑1 (MQ=255)

                                                                                                                    aTTAACCAGTTACTTACTTTACTACAATGTAACCGGCAGTGATTTTTTAAGAGCCATGCCTTCAGCAGAAATTTCCGCTTTGTAAGCCAGAATTTCTACCCCCCTCTGTTGAGCTTCTGACAATAGTTGCGCGTATTtct                  <  1:4824462/140‑1 (MQ=255)

                                                                                                                    aTTAACCAGTTACTTACTTTACTACAATGTAACCGGCAGTGATTTTTTAAGAGCCATGCCTTCAGCAGAAATTTCCGCTTTGTAAGCCAGAATTTCTACCCCCCTCTGTTGAGCTTCTGACAATAGTTGCGCGTATTtct                  <  2:5684404/140‑1 (MQ=255)

                                                                                                                    aTTAACCAGTTACTTACTTTACTACAATGTAACCGGCAGTGATTTTTTAAGAGCCATGCCTTCAGCAGAAATTTCCGCTTTGTAAGCCAGAATTTCTACCCCCCTCTGTTGAGCTTCTGACAATAGTTGCGCGTATTtct                  <  2:4306062/140‑1 (MQ=255)

                                                                                                                      tAACCAGTTACTTACTTTACTACAATGTAACCGGCAGTGATTTTTTAAGAGCCATGCCTTCAGCAGAAATTTCCGCTTTGTAAGCCAGAATTTCTACCCCCCTCTGTTGAGCTTCTGACAATAGTTGCGCGTATTTCTCa                <  1:5116724/140‑1 (MQ=255)

                                                                                                                       aaCCAGTTACTTACTTTACTACAATGTAACCGGCAGTGATTTTTTAAGAGCCATGCCTTCAGCAGAAATTTCCGCTTTGTAAGCCAGAATTTCTACCCCCCTCTGTTGAGCTTCTGACAATAGTTGCGCGTCTTTCTCAt               >  1:6657525/1‑140 (MQ=255)

                                                                                                                       aaCCAGTTACTTACTTTACTACAATGTAACCGGCAGTGATTTTTTAAGAGCCATGCCTTCAGCAGAAATTTCCGCTTTGTAAGCCAGAATTTCTACCCCCCTCTGTTGAGCTTCTGACAATAGTTGCGCGTATTTCTCAt               >  2:3514298/1‑140 (MQ=255)

                                                                                                                        aCCAGTTACTTACTTTACTACAATGTAACCGGCAGTGATTTTTTAAGAGCCATGCCTTCAGCAGAAATTTCCGCTTTGTAAGCCAGAATTTCTACCCCCCTCTGTTGAGCTTCTGACAATAGTTGCGCGTATTTCTCATc              >  1:4551851/1‑140 (MQ=255)

                                                                                                                        aCCAGTTACTTACTTTACTACAATGTAACCGGCAGTGATTTTTTAAGAGCCATGCCTTCAGCAGAAATTTCCGCTTTGTAAGCCAGAATTTCTACCCCCCTCTGTTGAGCTTCTGACAATAGTTGCGCGTATTTCTCATc              >  1:8299647/1‑140 (MQ=255)

                                                                                                                         ccAGTTACTTACTTTACTACAATGAAACCGGCAGTGATTTTTTAAGAGCCATGCCTTCAGCCGAAATTTCCGCTTTGTAAGCCAGAATTTCTACCCCCCTCTGTTGAGCTTCTGACAATAGTTGCGCGTATTTCTCATCg             >  2:4714319/1‑140 (MQ=255)

                                                                                                                           aGTTACTTACTTTACTACAATGTAACCGGCAGTGATTTTTTAAGAGCCATGCCTTCAGCAGAAATTTCCGCTTTGTAAGCCAGAATTTCTACCCCCCTCTGTTGAGCTTCTGACAATAGTTGCGCGTATTTCTCATCGAt           >  2:5783263/1‑140 (MQ=255)

                                                                                                                            ggtacttactTTACTACAATGTAACCGGCAGTGATTTTTTAAGAGCCATGCCTTCAGCAGAAATTTCCGCTTTGTAAGCCAGAATTTCTACCCCCCTCTGTTGAGCTTCTGACAATAGTTGCGCGTATTTCTCATCGAtg          <  2:1793898/138‑1 (MQ=255)

                                                                                                                             ttacttacTTTACTACAATGTAACCGGCAGTGATTTTTTAAGAGCCATGCCTTCAGCAGAAATTTCCGCTTTGTAAGCCAGAATTTCTACCCCCCTCTGTTGAGCTTCTGACAATAGTTGCGCGTATTTCTCATCGAtgt         >  2:5444472/1‑140 (MQ=255)

                                                                                                                              tacttactTTACTACAATGTAACCGGCAGTGATTTTTTAAGAGCCATGCCTTCAGCAGAAATTTCCGCTTTGTAAGCCAGAATTTCTACCCCCCTCTGTTGAGCTTCTGACAATAGTTGCGCGTATTTCTCATCGAtgtg        <  2:7984500/140‑1 (MQ=255)

                                                                                                                               acttacttTACTACAATGTAACCGGCAGTGATTTTTTAAGAGCCATGCCTTCAGCAGAAATTTCCGCTTTGTAAGCCAGAATTTCTACCCCCCTCTGTTGAGCTTCTGACAATAGTTGCGCGTATTTCTCATCGATGTgg       >  1:5483700/1‑140 (MQ=255)

                                                                                                                                   acttTACTACAATGTAACCGGCAGTGATTTTTTAAGAGCCATGCCTTCAGCAGAAATTTCCGCTTTGTAAGCCAGAATTTCTACCCCCCTCTGTTGAGCTTCTGACAATAGTTGCGCGTATTTCTCATCGATGTGgcgcg   <  2:2176857/140‑1 (MQ=255)

                                                                                                                                   acttTACTACAATGTAACCGGCAGTGATTTTTTAAGAGCCATGCCTTCAGCAGAAATTTCCGCTTTGTAAGCCAGAATTTCTACCCCCCTCTGTTGAGCTTCTGACAATAGTTGCGCGTATTTCTCATCGATGTGgcgcg   <  1:2386612/140‑1 (MQ=255)

                                                                                                                                   acttTACTACAATGTAACCGGCAGTGATTTTTTAAGAGCCATGCCTTCAGCAGAAATTTCCGCTTTGTAAGCCAGAATTTCTACCCCCCTCTGTTGAGCTTCTGACAATAGTTGCGCGTATTTCTCATCGATGTGgcgcg   <  1:2942255/140‑1 (MQ=255)

                                                                                                                                   acttTACTACAATGTAACCGGCAGTGATTTTTTAAGAGCCATGCCTTCAGCAGAAATTTCCGCTTTGTAAGCCAGAATTTCTACCCCCCTCTGTTGAGCTTCTGACAATAGTTGCGCGTATTTCTCATCGATGTGgcgcg   <  2:4559807/140‑1 (MQ=255)

                                                                                                                                   acttTACTACAATGTAACCGGCAGTGATTTTTTAAGAGCCATGCCTTCAGCAGAAATTTCCGCTTTGTAAGCCAGAATTTCTACCCCCCTCTGTTGAGCTTCTGACAATAGTTGCGCGTATTTCTCATCGATGTGgcgcg   <  1:7204437/140‑1 (MQ=255)

                                                                                                                                   acttTACTACAATGTAACCGGCAGTGATTTTTTAAGAGCCATGCCTTCAGCAGAAATTTCCGCTTTGTAAGCCAGAATTTCTACCCCCCTCTGTTGAGCTTCTGACAATAGTTGCGCGTATTTCTCATCGATGTGgcgcg   <  1:6185042/140‑1 (MQ=255)

                                                                                                                                   acttTACTACAATGTAACCGGCAGTGATTTTTTAAGAGCCATGCCTTCAGCAGAAATTTCCGCTTTGTAAGCCAGAATTTCTACCCCCCTCTGTTGAGCTTCTGACAATAGTTGCGCGCATTTCTCATCGATGTGgcgcg   <  1:7697951/140‑1 (MQ=255)

GGGGAAAAATGAGGCCGCTATAAATAGCAGATGCTTTTCCGGATAGCAATTATCTAAACGTAACACTTGACAACTGTGTGAGGAAAAGCGTATTTGCGCACGCGACCAGAATGTAAATTAACCAGTTACTTACTTTATTACAATGTAACCGGCAGTGATTTTTTAAGAGCCATGCCTTCAGCAGAAATTTCCGCTTTGTAAGCCAGAATTTCTACCCCCCTCTGTTGAGCTTCTGACAATAGTTGCGCGTATTTCTCATCGATGTGGCGCGC  >  NZ_CP025268/160476‑160747

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`