BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP025268	14,302	T→C	V45A (GTG→GCG)	dnaJ →	molecular chaperone DnaJ

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP025268	14,302	0	T	C	99.6%	1586.4 / NA	459	V45A (GTG→GCG)	dnaJ	molecular chaperone DnaJ
Reads supporting (aligned to +/- strand): ref base T (0/0); major base C (245/212); minor base A (1/1); total (246/213)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 459 total aligned reads displayed.

AAAGATGGCTAAGCAAGATTATTACGAGATTTTAGGCGTTTCCAAAACAGCGGAAGAGCGTGAAATCAGAAAGGCCTACAAACGCCTGGCCATGAAATACCACCCGGACCGTAACCAGGGTGACAAAGAGGCCGAGGTGAAATTTAAAGAGATCAAGGAAGCTTATGAAGTTCTGACCGACTCGCAAAAACGTGCGGCATACGATCAGTATGGTCATGCTGCGTTTGAGCAAGGTGGCATGGGCGGCGGCGGTTTTGGCGGCGGCGCAGACT  >  NZ_CP025268/14165‑14436

        cTAAGCAAGATTATTACGAGATTTTAGTCGTGTCCAAAACAGCGGAAGAGCGTGAAATCAGAAAGGCATACAAACGCCTGGCCATGAAATACCACCCGGACCGTAACCAGGGTGAAAAAGAGGCCGAGGCGAAATTTaaa                                                                                                                              <  2:2261887/140‑1 (MQ=255)

        cTAAGCAAGATTATTACGAGATTTTAGGCGTTTCCAAAACAGCGGAAGAGCGTGAAATCAGAAAGGCCTACAAACGCCTGGCCATGAAATCCCACCCGGACCGTAACCAGGTTGACAAAGAGGCCGAGGCGAAATTTaaa                                                                                                                              <  2:1924533/140‑1 (MQ=255)

        cTAAGCAAGATTATTACGAGATTTTAGGCGTTTCCAAAACAGCGGAAGAGCGTGAAATCAGAAAGGCCTACAAACGCCTGGCCATGAAATACCACCCGGACCGTAACCAGGGTGACAAAGAGGCCGAGGCGAAATTTaaa                                                                                                                              <  1:8469921/140‑1 (MQ=255)

        cTAAGCAAGATTATTACGAGATTTTAGGCGTTTCCAAAACAGCGGAAGAGCGTGAAATCAGAAAGGCCTACAAACGCCTGGCCATGAAATACCACCCGGACCGTAACCAGGGTGACAAAGAGGCCGAGGCGAAATTTaaa                                                                                                                              <  1:483090/140‑1 (MQ=255)

        cTAAGCAAGATTATTACGAGATTTTAGGCGTTTCCAAAACAGCGGAAGAGCGTGAAATCAGAAAGGCCTACAAACGCCTGGCCATGAAATACCACCCGGACCGTAACCAGGGTGACAAAGAGGCCGAGGCGAAATTTaaa                                                                                                                              <  2:7643056/140‑1 (MQ=255)

        cTAAGCAAGATTATTACGAGATTTTAGGCGTTTCCAAAACAGCGGAAGAGCGTGAAATCAGAAAGGCCTACAAACGCCTGGCCATGAAATACCACCCGGACCGTAACCAGGGTGACAAAGAGGCCGAGGCGAAATTTaaa                                                                                                                              <  1:2889919/140‑1 (MQ=255)

        cTAAGCAAGATTATTACGAGATTTTAGGCGTTTCCAAAACAGCGGAAGAGCGTGAAATCAGAAAGGCCTACAAACGCCTGGCCATGAAATACCACCCGGACCGTAACCAGGGTGACAAAGAGGCCGAGGCGAAATTTaaa                                                                                                                              <  1:2582714/140‑1 (MQ=255)

           aGCAAGATTATTACGAGATTTTAGGCGTTTCCAAAACAGCGGACGAGCGTGAAATCAGAAAGGCCTACAAACGCCTGGCCATGAAATACCACCCGGACCGTAACCAGGGTGACAATGAGGCCGAGGCGAAATTTAAagag                                                                                                                           >  2:1997656/1‑140 (MQ=255)

           aGCAAGATTATTACGAGATTTTAGGCGTTTCCAAAACAGCGGAAGAGCGTGAAATCAGAAAGGCCTACAAACGCCTGGCCATGAAATACCACCCGGACCGTAACCAGGGTGACAAAGAGGCCGAGGCGAAATTTAAagag                                                                                                                           <  2:3699827/140‑1 (MQ=255)

           aGCAAGATTATTACGAGATTTTAGGCGTTTCCAAAACAGCGGAAGAGCGTGAAATCAGAAAGGCCTACAAACGCCTGGCCATGAAATACCACCCGGACCGTAACCAGGGTGACAAAGAGGCCGAGGCGAAATTTAAagag                                                                                                                           <  1:5204829/140‑1 (MQ=255)

           aGCAAGATTATTACGAGATTTTAGGCGTTTCCAAAACAGCGGAAGAGCGTGAAATCAGAAAGGCCTACAAAAGCCTGGCCATGAAATACCACCCGGACCGTAACCAGGGTGACAAAGAGGCCGAGGCGAAATTTAAagag                                                                                                                           >  1:7812110/1‑140 (MQ=255)

                 tttcttaCGAGATTGTAGTCGTTTACAAAACAGCGGAAGAGCGTGAAATCAGAAAGGACTACAAAAGCCTTGCCATGAAATACAACCCGGACCGTAAACAGGGTGACAAAGAGGCCGAGGAGAAATTTAAAGAGCTTAAg                                                                                                                     <  2:4017133/136‑1 (MQ=255)

                 attattACGAGATTTTAGGCGTTTCCAAAACAGCGGAAGAGCGTGAAATCAGAAAGGCCTACAAACGCCTGGCCATGAAATACCACCCGGACCGTAACCAGGGTGACAAAGAGGCCGAGGCGAAATTTAAAGAGATCAAg                                                                                                                     >  2:5261379/1‑140 (MQ=255)

                 attattACGAGATTTTAGGCGTTTCCAAAACAGCGGAAGAGCGTGAAATCAGAAAGGCCTACAAACGCCTGGCCATGAAATACCACCCGGACCGTAACCAGGGTGACAAAGAGGCCGAGGCGAAATTTAAAGAGATCAAg                                                                                                                     >  1:5603201/1‑140 (MQ=255)

                   tattaCGAGATTTTAGGCGTTTCCAAAACAGCGGAAGAGCGTGAAATCAGAAAGGCCTACAAACGCCTGGCCATGAAATACCACCCGGACCGTAACCAGGGTGACAAAGAGGCCGAGGCGAAATTTAAAGAGATCAAGGa                                                                                                                   <  1:5097921/140‑1 (MQ=255)

                   tattaCGAGAGATTAGGCCTTTCCACAACAGCGGAAGAGAGAGAAATCACACAGGCCTACAAACGCCTGGCCATGACATACCACCCGGACCGTAACCAGGGTGCCACAGCGGCCGAGCCGAAATTTAAAGCGATCAAGGa                                                                                                                   <  2:8139908/140‑1 (MQ=255)

                   gatttCGAGATTTTAGGCGTTTCCAAAACAGCGGAAGAGCGTGAAATCAGAAAGGCCTACAAACGCCTGTCCATGAAATACCACCCGGACCGTAACCAGGGTGACAAAGATGCCGATGCGAAATTTAAAGAGATCAAGGa                                                                                                                   <  2:8995702/139‑1 (MQ=255)

                      gaCGAGACGTCAGGCGGTTCCAAAACAGCGTAAGAGCGTGAAATCAGAAAGGCCTACAAACGCCTGGCCATGAAATACCACCCGGACCGTAACCAGGGTTACAAAGAGGCCGAGGCGAAATTTAAAGAGATCAAGGAAGc                                                                                                                <  2:6702079/139‑1 (MQ=255)

                        cGAGATTTTAGGCGTTTCCAAAACAGCGGAAGAGCGTGAAATCAGAAAGGCCTACAAACGCCTGGCCATGAAATACCACCCGGACCGTAACCAGGGTGACAAAGAGGCCGAGGCGAAATTTAAAGAGATCAAGGACGCtt                                                                                                              >  2:4716468/1‑140 (MQ=255)

                          agaTTTTAGGCGTTTCCAAAACAGCGGAAGAGCGTGAAATCAGAAAGGCCTACAAACGCCTGGCCATGAAATACCACCCGGACCGTAACCAGGGTGACAAAGAGGCCGAGGCGAAATTTAAAGAGATCAAGGACGCTTAt                                                                                                            >  1:6431127/1‑140 (MQ=255)

                                      tttCCAAAACAGCGGAAGAGCGTGAAATCAGAAAGGCCTACAAACGCCTGGCCATGAAATACCACCCGGACCGTAACCAGGGTGACAAAGAGGCCGAGGCGAAATTTAAACAGATCAAGGAAGCTTATGAAGTTCTGAcc                                                                                                >  1:1806580/1‑140 (MQ=255)

                                       ttCCAAAACAGCGGAAGAGCGTGAAATCAGACAGGCCTACAAACGCCTGGCCATGAAATACCACCCGGACCGTAACCAGGGTGACAAAGAGGCCGAGGCGAAATTTAAAGAGATCAAGGAAGCTTATGAAGTTCTGACCg                                                                                               >  1:4156987/1‑140 (MQ=255)

                                       ttCCAAAACAGCGGAAGAGCGTGAAATCAGAAAGGCCTACAAACGCCTGGCCATGAAATACCACCCGGACCGTAACCAGGGTGACAAAGAGGCCGAGGCGAAATTTAAAGAGATCAAGGAAGCTTATGAAGTTCTGACCg                                                                                               >  1:9301018/1‑140 (MQ=255)

                                        tCCAAAACAGCGGAAGAGCGTGAAATCAGAAAGGCCTACAAACGCCTGGCCATGAAATACCACCCGGACCGTAACCAGGGTGACAAAGAGGCCGAGGCGAAATTTAAAGAGATCAAGGAAGCTTATGAAGTTCTGACCGa                                                                                              >  1:546307/1‑140 (MQ=255)

                                         ccAAAACAGCGGAAGAGCGTGAAATCAGAAAGGCCTACAAACGCCTGGCCATGAAATACCACCCGGACCGTAACCAGGGTGACAAAGAGGCCGAGTCGAAATTTAAAGAGATCAAGGAAGCTTATGAAGTTCTGACCGAc                                                                                             <  2:3890151/140‑1 (MQ=255)

                                              aCAGCGGAAGAGCGTGAAATCAGAAAGGCCTACAAACGCCTGGCCATGAAATACCACCCGGACCGTAACCAGGGTGACAAAGAGGCCGAGGCGAAATTTAAAGAGATCAAGGAAGCTTATGAAGTTCTGACCGACTCGCa                                                                                        <  1:3922221/140‑1 (MQ=255)

                                              aCAGCGGAAGAGCGTGAAATCAGAAAGGCCTACAAACGCCTGGCCATGAAATACCACCCGGACCGTAACCAGGGTGACAAAGAGGCCGAGGCGAAATTTAAAGAGATCAAGGAAGCTTATGAAGTTCTGACCGACTCGCa                                                                                        <  1:8652056/140‑1 (MQ=255)

                                                 gCGGAAGAGCGTGAAATCAGAAAGGCCTACAAACGCCTGGCCATGAAATACCACCCGGACCGTAACCAGGGTGACAAAGAGGCCGAGGCGAAATTTAAAGAGATCAAGGAAGCTTATGAAGTTCTGACCGACTCGCaaaa                                                                                     >  1:1002525/1‑140 (MQ=255)

                                                 gCGGAAGAGCGTGAAATCAGAAAGGCCTACAAACGCCTGGCCATGAAATACCACCCGGACCGTAACCAGGGTGACAAAGAGGCCGAGGCGAAATTTAAAGAGATCAAGGAAGCTTATGAAGTTCTGACCGACTCGCaaaa                                                                                     >  1:7283510/1‑140 (MQ=255)

                                                 gCGGAAGAGCGTGAAATCAGAAAGGCCTACAAACGCCTGGCCATGAAATACCACCCGGACCGTAACCAGGGTGACAAAGAGGCCGAGGCGAAATTTAAAGAGATCAAGGAAGCTTATGAAGTTCTGACCGACTCGCaaaa                                                                                     >  2:1586945/1‑140 (MQ=255)

                                                 gCGGAAGAGCGTGAAATCAGAAAGGCCTACAAACGCCTGGCCATGAAATACCACCCGGACCGTAACCAGGGTGACAAAGAGGCCGAGGCGAAATTTAAAGAGATCAAGGAAGCTTATGAAGTTCTGACCGACTCGCaaaa                                                                                     >  1:1686491/1‑140 (MQ=255)

                                                  cGGAAGAGCGTGAAATCAGAAAGGCCTACAAACGCCTGGCCATGAAATACCACCCGGACCGTAACCAGGGTGACAAAGAGGCCGAGGCGAAATTTAAAGAGATCAAGGAAGCTTATGAAGTTCTGACCGACTCGCaaaaa                                                                                    >  1:234626/1‑140 (MQ=255)

                                                  cGGAAGAGCGTGAAATCAGAAAGGCCTACAAACGCCTGGCCATGAAATACCACCCGGACCGTAACCAGGGTGACAAAGAGGCCGAGGCGAAATTTAAAGAGATCAAGGAAGCTTATGAAGTTCTGACCGACTCGCaaaaa                                                                                    >  1:8520972/1‑140 (MQ=255)

                                                  cGGAAGAGCGTGAAATCAGAAAGGCCTACAAACGCCTGGCCATGAAATACCACCCGGACCGTAACCAGGGTGACAAAGAGGCCGAGGCGAAATTTAAAGAGATCAAGGAAGCTTATGAAGTTCTGACCGACTCGCaaaaa                                                                                    >  1:8333224/1‑140 (MQ=255)

                                                  cGGAAGAGCGTGAAATCAGAAAGGCCTACAAACGCCTGGCCAAGAAAGACCACCCGGACCCTAACCAGGGTGACAAAGAGGCCGAGGCGAAAATTAAAGAGATCAAGCAAGCTTATGCAGTTCTGACCGACTCGCaaaaa                                                                                    >  2:1141872/1‑140 (MQ=255)

                                                  cGGAAGAGCCTGAAATCAGAAAGGCCTACAAACGCCTGGCCATGAAATACCACCCGGACCGTAACCCGGGTGACAATGAGGCCGAGGCGACATTTAAAGAGATCAAGGAAGCTTATGAAGTTCTTACCGACTCGCaaaaa                                                                                    >  2:3507879/1‑140 (MQ=255)

                                                      agagCGTGAAATCAGAAAGGCCTACAAACGCCTGGCCATGAAATACCACCCGGACCGTAACCAGGGTGACAAAGAGGCCGAGGCGAAATTTAAAGAGATCAAGGAAGCTTATGAAGTTCTGACCGACTCGCAAAAACGTg                                                                                <  2:6162196/140‑1 (MQ=255)

                                                      agagCGTGAAATCAGAAAGGCCTACAAACGCCTGGCCATGAAATACCACCCGGACCGTAACCAGGGTGACAAAGAGGCCGAGGCGAAATTTAAAGAGATCAAGGAAGCTTATGAAGTTCTGACCGACTCGCAAAAACGTg                                                                                <  2:5953540/140‑1 (MQ=255)

                                                       gagCGTGAAATCAGAAAGGCCTACAAACGCCTGGCCATGAAATACCACCCGGACCGTAACCAGGGTGACAAAGCGGCCGAGGCGAAATTTAAAGAGATCAAGGAAGCTTATGAAGTTCTGACCGACTCGCAAAAACGTGc                                                                               <  2:8583657/140‑1 (MQ=255)

                                                       gagCGTGAAATCAGAAAGGCCTACAAACGCCTGGCCATGAAATACCACCCGGACCGTAACCAGGGTGACAAAGAGGCCGAGGCGAAATTTAAAGAGATCAAGGAAGCTTATGAAGTTCTGACCGACTCGCAAAAACGTGc                                                                               <  1:2029875/140‑1 (MQ=255)

                                                       gagCGTGAAATCAGAAAGGCCTACAAACGCCTGGCCATGAAATACCACCCGGACCGTAACCAGGGTGACAAAGAGGCCGAGGCGAAATTTAAAGAGATCAAGGAAGCTTATGAAGTTCTGACCGACTCGCAAAAACGTGc                                                                               <  1:2123709/140‑1 (MQ=255)

                                                         gCGTGAAATCAGAAAGGCCTACAAACGCCTGGCCATGAAATACCACCCGGACCGTAACCAGGGTGACAAAGAGGCCGAGGCGAAATTTAAAGAGATCAAGGAAGCTTATGAAGTTCTGACCGACTCGCAAAAACGTGCgg                                                                             <  1:325557/140‑1 (MQ=255)

                                                         gCGTGAAATCAGAAAGGCCTACAAACGCCTGGCCATGAAATACCACCCGGACCGTAACCAGGGTGACAAAGAGGCCGAGGCGAAATTTAAAGAGATCAAGGAAGCTTATGAAGTTCTGACCGACTCGCAAAAACGTGCgg                                                                             >  2:1384613/1‑140 (MQ=255)

                                                            tGAAATCAGAACGGCCTACAAACGCCTGGCCATGAAATACCACCCGGACCGTAACCAGGGTGACAACGAGGCCGAGGCGAAATTTAAAGAGATCAAGGAAGCTTATGAAGTTCTGACCGACTCGCAAAAACGTGCGGCAt                                                                          <  1:2985317/140‑1 (MQ=255)

                                                            tGAAATCAGAAAGGCCTACAAACGCCTGGCCATGAAATACCACCCGGACCGTAACCAGGGTGACAAAGAGGCCGAGGCGAAATTTAAAGAGATCAAGGAAGCTTATGAAGTTCTGACCGACTCGCAAAAACGTGCGGCAt                                                                          <  2:7289245/140‑1 (MQ=255)

                                                               aaTCAGAAAGGCCTACAAACGCCTGGCCATGAAATACCACCCGGACCGTAACCAGGGTGACAAAGAGGCCGAGGCGAAATTTAAAGAGATCAAGGAAGCTTATGAAGTTCTGACCGACTCGCAAAAACGTGCGGCATACg                                                                       <  2:5490037/140‑1 (MQ=255)

                                                               aaTCAGAAAGGCCTACAAACGCCTGGCCATGAAATACCACCCGGACCGTAACCAGGGTGACAAAGAGGCCGAGGCGAAATTTAAAGAGATCAAGGAAGCTTATGAAGTTCTGACCGACTCGCAAAAACGTGCGGCATACg                                                                       <  2:205864/140‑1 (MQ=255)

                                                               aaTCAGAAAGGCCTACAAACGCCTGGCCATGAAATACCACCCGGACCGTAACCAGGGTGACAAAGAGGCCGAGGCGAAATTTAAAGAGATCAAGGAAGCTTATGAAGTTCTGACCGACTCGCAAAAACGTGCGGCATACg                                                                       <  2:5142346/140‑1 (MQ=255)

                                                                aTCAGAAAGGCCTACCAACGCCTGGCCATGAAATACCACCCGGACCCTAACCAGGGTGACAAAGAGGCCGAGGCGAAATTTAAAGAGATCAAGGAAGCTTATGAAGTTCTGACCGACTCGCAAAAACGTGCGGCATACGa                                                                      <  2:9278949/140‑1 (MQ=255)

                                                                aTCAGAAAGGCCTACAAACGCCTGGCCATGAAATACCACCCGGACCGTAACCAGGGTGACAAAGAGGCCGAGGCGAAATTTAAAGAGATCAAGGAAGCTTATGAAGTTCTGACCGACTCGCAAAAACGTGCGGCATACGa                                                                      <  2:2852044/140‑1 (MQ=255)

                                                                 tCAGAAAGGCCTTCAAACGCCTGGCCATGAAATACCACCCGGACCGTAACCAGGGTGACAAAGAGGCCGAGGCGAAATTTAAAGAGATCAAGGAAGCTTAAGAAGTTCTGACCGAATCGCAAAAACGTGCGGCATACGAt                                                                     <  1:5857811/140‑1 (MQ=255)

                                                                 tCAGAAAGGCCTACAAACGCCTGGCCATGAAATACCACCCGGACCGTAACCAGGGTGACAAAGAGGCCGAGGCGAAATTTAAAGAGATCAAGGAAGCTTATGAAGTTCTGACCGACTCGCAAAAACGTGCGGCATACGAt                                                                     <  2:1308299/140‑1 (MQ=255)

                                                                      aaGGCCTACAAACGCCTGGCCATGAAATACCACCCGGACCGTAACCAGGGTGACAAAGAGGCCGAGGCGAAATTTAAAGAGATCAAGGAAGCTTATGAAGTTCTGACCGACTCGCAAAAACGTGCGGCATACGATCAGTa                                                                <  1:7097474/140‑1 (MQ=255)

                                                                      aaGGCCTACAAACGCCTGGCCATGAAATACCACCCGGACCGTAACCAGGGTGACAAAGAGGCCGAGGCGAAATTTAAAGAGATCAAGGAAGCTTATGAAGTTCTGACCGACTCGCAAAAACGTGCGGCATACGATCAGTa                                                                <  2:1731606/140‑1 (MQ=255)

                                                                          ccTACAAACGCCTGGCCATGAAATACCACCCGGACCGTAACCAGGGTGACAAAGAGGCCGAGGCGAAATTTAATGAGATCAAGGAAGCTTATGAAGTTCTGACCGACTCGCAAAAACGTGCGGCATACGATCAGTATGGt                                                            <  1:1423782/140‑1 (MQ=255)

                                                                            tACAAACGCCTGGCCATGAAATACCACCCGGACCGTAACCAGGGTGACAAAGAGGCCGAGGCGAAATTTAAAGAGATCAAGGAAGCTTATGAAGTTCTGACCGACTCGCAAAAACGTGCGGCATACGATCAGTATGGTCa                                                          >  2:6225166/1‑140 (MQ=255)

                                                                             aCAAACGCCTGGCCATGAAATACCACCCGGACCGTAACCAGGGTGACAAAGAGGCCGAGGCGAAATTTAAAGAGATCAAGGAAGCTTATGAAGTTCTGACCGACTCGCAAAAACGTGCGGCATACGATCAGTATGGTCAt                                                         >  2:6491769/1‑140 (MQ=255)

                                                                              cAAACGCCTGGCCATGAAATACCACCCGGACCGTAACCAGGGTGACAAAGAGGCCGAGGCGAAATTTAAAGAGATCAAGGAAGCTTATGAAGTTCTGACCGACTCGCAAAAACGTGCGGCATACGATCAGTATGGTCAtg                                                        <  1:7401916/140‑1 (MQ=255)

                                                                               aaaCGCCTGGCCATGAAATACCCCCCGGACCGTAACCAGGGTGACAAAGAGGCCGAGGCGAAATTTAAAGAGATCAAGGAAGCTTATGAAGTTCTGACCGACTCGCAAAAACGTGCGGCATACGATCAGTATGGTCAtgc                                                       <  2:3322445/140‑1 (MQ=255)

                                                                               aaaCGCCTGGCCATGAAATACCACCCGGACCGTAACCAGGGTGACAAAGAGGCCGAGGCGAAATTTAAAGAGATCAAGGAAGCTTATGAAGTTCTGACCGACTCGCAAAAACGTGCGGCATACGATCAGTATGGTCAtgc                                                       <  1:3448922/140‑1 (MQ=255)

                                                                                 aCGCCTGGCCATGAAATACCACCCGGACCGTAACCAGGGTGACAAAGAGGCCGAGGCGAAATTTAAAGAGATCAAGGAAGCTTATGAAGTTCTGACCGACTCGCAAAAACGTGCGGCATACGATCAGTATGGTCAtgctg                                                     <  1:7696837/140‑1 (MQ=255)

                                                                                   gCCTGGCCATGAAATACCACCCGGACCGTAACCAGGGTGACAAAGAGGCCGAGGCGAAATTTAAAGAGATCAAGGAAGCTTATGAAGTTCTGACCGACTCGCAAAAACGTGCGGCATACGATCAGTATGGTCATGCTGCg                                                   >  2:7738582/1‑140 (MQ=255)

                                                                                   gCCAGGCCATGAAATACCAACCGGACCGTAACCAGGGTGACAAAGTGGCCGAGGCGAAATTTAAAGAGATCAAGGAAGCTTATGAAGTTCTGACCGACTCGCAAAAACGTGCGGCAAACGATCAGTCTGGTCATGCTGCg                                                   >  2:8835762/1‑140 (MQ=255)

                                                                                    ccTGGCCATGAAATACCACCCGGACCGTAACCAGGGTGACAAAGAGGCCGAGGCGAAATTTAAAGAGATCAAGGAAGCTTATGAAGTTCTGACCGACTCGCAAAAACGTGCGGCATACGATCAGTATGGTCATGCTGCGt                                                  >  2:5647706/1‑140 (MQ=255)

                                                                                    ccTGGCCATGAAATACCACCCGGACCGTAACCAGGGTGACAAAGAGGCCGAGGCGAAATTTAAAGAGATCAAGGAAGCTTATGAAGTTCTGACCGACTCGCAAAAACGTGCGGCATACGATCAGTATGGTCATGCTGCGt                                                  >  2:4384628/1‑140 (MQ=255)

                                                                                    ccTGGCCATGAAATACCACCCGGACCGTAACCAGGGTGACAAAGAGGCCGAGGCGAAATTTAAAGAGATCAAGGAAGCTTATGAAGTTCTGACCGACTCGCAAAAACGTGCGGCATACGATCAGTATGGTCATGCTGCGt                                                  >  2:7412441/1‑140 (MQ=255)

                                                                                      tGGCCATGAAATACCACCCGGACCGTAACCAGGGTGACAAAGAGGCCGAGGCGAAATTTAAAGAGATCAAGGAAGCTTATGAAGTTCTGACCGACTCGCAAAAACGTGCGGCATACGATCAGTATGGTCATGCTGCGttt                                                >  1:5272652/1‑140 (MQ=255)

                                                                                        gCCATGAAATACCACCCGGACCGTAACCAGGGTGACAAAGAGGCCGAGGCGAAATTTAAAGAGATCAAGGAAGCTTATGAAGTTCTGACCGACTCGCAAAAACGTGCGGCATACGATCAGTATGGTCATGCTGCGTTTGa                                              >  2:5850858/1‑140 (MQ=255)

                                                                                         ccATGAAATACCACCCGGACCGTAACCAGGGTGACAAAGAGGCCGAGGCGAAATTTAAAGAGATCAAGGAAGCTTATGAAGTTCTGACCGACTCGCAAAAACGTGCGGCATACGATCAGTATGGTCATGCTGCGTTTGAg                                             <  2:4835001/140‑1 (MQ=255)

                                                                                             gAAATACCACCCGGACCGTAACCAGGGTGACAAAGCCGCCGAGGCGAAATTTAAAGAGATCAAGGAAGCTTATGAAGTTCTGACCGACTCGCAAAAACGTGCGGCATACGATCAGTATGGTCATGCTGCGTTTGAGCAAg                                         <  1:6214376/140‑1 (MQ=255)

                                                                                             gAAATACCACCCGGACCGTAACCAGGGTGACAAAGAGGCCGAGGCGAAATTTaaa                                                                                                                              <  1:6099771/55‑1 (MQ=255)

                                                                                             gAAATACCACCCGGACCGTAACCAGGGTGACAAAGAGGCCGAGGCGAAATTTAAAGAGATCAAGGAAGCTTATGAAGTTCTGACCGACTCGCAAAAACGTGCGGCATACGATCAGTATGGTCATGCTGCGTTTGAGCAAg                                         <  1:6952001/140‑1 (MQ=255)

                                                                                                   ccaccCGGACCGTAACCAGGGTGACAAAGAGGCCGAGGCGAAATTTAAAGAGATCAAGGAAGCTTATGAAGTTCTGACCGACTCGCAAAAACGTGCGGCATACGATCAGTATGGTCATGCTGCGTTTGAGCAAGGTGGCa                                   >  1:4611629/1‑140 (MQ=255)

                                                                                                      cccGGACCGTAACCAGGGTGACAAAGAGGCCGAGGCGAAATTTAAAGAGATCAAGGAAGCTTATGAAGTTCTGACCGACTCGCAAAAACGTGCGGCATACGATCAGTATGGTCATGCTGCGTTTGAGCAAGGTGGCATgg                                >  1:976917/1‑140 (MQ=255)

                                                                                                      cccGGACCGTAACCAGGGTGACAAAGAGGCCGAGGCGAAATTTAAAGAGATCAAGGAAGCTTATGAAGTTCTGACCGACTCGCAAAAACGTGCGGCATACGATCAGTATGGTCATGCTGCGTTTGAGCAAGGTGGCATgg                                >  1:1328559/1‑140 (MQ=255)

                                                                                                      cccGGACCGTAACCAGGGTGACAAAGAGGCCGAGGCGAAATTTAAAGAGATCAAGGAAGCTTATGAAGTTCTGACCGACTCGCAAAAACGTGCGGCATACGATCAGTATGGTCATGCTGCGTTTGAGCAAGGTGGCATgg                                >  2:2389069/1‑140 (MQ=255)

                                                                                                         ggACCGTAACCAGGGTGACAAAGAGGCCGAGGCGAAATTTAAAGAGATCAAGGAAGCTTATGAAGTTCTGACCGACTCGCAAAAACGTGCGGCATACGATCAGTATGGTCATGCTGCGTTTGAGCAAGGTGGCATGggcg                             >  1:3097765/1‑140 (MQ=255)

                                                                                                         ggACCGTAACCAGGGTGACAAAGAGGCCGAGGCGAAATTTAAAGAGATCAAGGAAGCTTATGAAGTTCTGACCGACTCGCAAAAACGTGCGGCATACGATCAGTATGGTCATGCTGCGTTTGAGCAAGGTGGCATGggcg                             >  1:6038174/1‑140 (MQ=255)

                                                                                                           aCCGTAACCAGGGTGACAAAGAGGCCGAGGCGAAATTTAAAGAGATCAAGGAAGCTTATGAAGTTCTGACCGACTCGCAAAAACGTGCGGCATACGATCAGTATGGTCATGCTGCGTTTGAGCAAGGTGGCATGggcggc                           >  2:4970972/1‑140 (MQ=255)

                                                                                                           aCCGTAACCAGGGTGACAAAGAGGCCGAGGCGAAATTTAAAGAGATCAAGGAAGCTTATGAAGTTCTGACCGACTCGCAAAAACGTGCGGCATACGATCAGTATGGTCATGCTGCGTTTGAGCAAGGTGGCATGggcggc                           >  1:1268094/1‑140 (MQ=255)

                                                                                                              gTAACCAGGGTGACAAAGAGGCCGAGGCGAAATTTAAAGAGATCAAGGAAGCTTATGAAGTTCTGACCGACTCGCAAAAACGTGCGGCTTACGATCAGTATGGTCATGCTGCGTTTGAGCAAGGTGGCATGggcggcggc                        >  2:8360085/1‑140 (MQ=255)

                                                                                                               tAACCAGGGTGACAAAGAGGCCGAGGCGAAATTTAAAGAGATCAAGGAAGCTTATGAAGTTCTGACCGACTCGCAAAATCGTGCGGCATACGATCAGTATGGTCATGCTGCGTTTGAGCAAGGTGGCATGGgcggcggcg                       >  1:8958413/1‑140 (MQ=255)

                                                                                                               tAACCAGGGTGACAAAGAGGCCGAGGCGAAATTTAAAGAGATCAAGGAAGCTTATGAAGTTCTGACCGACTCGCAAAAACGTGCGGCATACGATCAGTATGGTCATGCTGCGTTTGAGCAAGGTGGCATGGgcggcggcg                       >  1:2201000/1‑140 (MQ=255)

                                                                                                               tAACCAGGGTGACAAAGAGGCCGAGGCGAAATTTAAAGAGATCAAGGAAGCTTATGAAGTTCTGACCGACTCGCAAAAACGTGCGGCATACGATCAGTATGGTCATGCTGCGTTTGAGCAAGGTGGCATGGgcggcggcg                       >  2:6843647/1‑140 (MQ=255)

                                                                                                               tAACCAGGGTGACAAAGAGCCCGAGGCGAAATTTAACGAGATCAAGGACGCTTATGAAGTTCGGACCGACTCGCAAAAACGTGCGGCATACGATCAGTATGGTCATGCTGCGTTTGAGCAAGGTGGCATGGgcggcggcg                       <  2:307171/140‑1 (MQ=255)

                                                                                                                 aCCAGGGTGACAAAGAGGCCGAGGCGAAATTTAAAGAGATCAAGGAAGCTTATGAAGTTCTGACCGACTCGCAAAAACGTGCGGCATACGATCAGTATGGTCATGCTGCGTTTGAGCAAGGTGGCATGGGCGGCGGCGGt                     >  2:6970047/1‑140 (MQ=255)

                                                                                                                 aCCAGGGTGACAAAGAGGCCGAGGCGAAATTTAAAGAGATCAAGGAAGCTTATGAAGTTCTGACCGACTCGCAAAAACGTGCGGCATACGATCAGTATGGTCATGCTGCGTTTGAGCAAGGTGGCATGGGCGGCGGCGGt                     >  2:2484343/1‑140 (MQ=255)

                                                                                                                 aCCAGGGTGACAAAGAGGCCGAGGCGAAAGTTAAAGAGATCAAGGAAGCTTATGAAGTTCTGACCGACTCGCAAAAACGTGCGGCATACGATCAGTATGGTCATGCTGCGTTTGAGCAAGGTGGCATGGGCGGCGGCGGt                     >  1:3132924/1‑140 (MQ=255)

                                                                                                                       gTGACAAAGAGGCCGAGGCGAAATTTAAAGAGATCAAGGAAGCTTATGAAGTTCTGACCGACTCGCAAAAACGTGCGGCATACGATCAGTATGGTCATGCTGCGTTTGAGCAAGGTGGCATGGGCGGCGGCGGTTTTggc               >  1:6786713/1‑140 (MQ=255)

                                                                                                                       gTGACAAAGAGGCCGAGGCGAAATTTAAAGAGATCAAGGAAGCTTATGAAGTTCTGACCGACTCGCAAAAACGTGCGGCATACGATCAGTATGGTCATGCTGCGTTTGAGCAAGGTGGCATGGGCGGCGGCGGTTTTggc               >  2:2715263/1‑140 (MQ=255)

                                                                                                                         gACAAAGAGGCCGAGGCGAAATTTACAGAGATCAAGGAAGCTTAAGAAGTTCTGACCGACTCGCAAAAACGTGCGGCATACGATCAGTATGGTCATGCTGCGTTTGAGCAAGGTGGCATGGGCGGCGGCGGTTTTggcgg             <  1:3658961/140‑1 (MQ=255)

                                                                                                                         gACAAAGAGGCCGAGGCGAAATTTAAAGAGATCAAGGAAGCTTATGAAGTTCTGACCGACTCGCAAAAACGTGCGGCATACGATCAGTATGGTCATGCTGCGTTTGAGCAAGGTGGCATGGTCGGCGGCGGTTTTggcgg             >  2:7066484/1‑140 (MQ=255)

                                                                                                                         gACAAAGAGGCCGAGGCGAAATTTAAAGAGATCAAGGAAGCTTATGAAGTTCTGACCGACTCGCAAAAACGTGCGGCATACGATCAGTATGGTCATGCTGCGTTTGAGCAAGGTGGCATGGGCGGCGGCGGTTTTggcgg             >  1:4251582/1‑140 (MQ=255)

                                                                                                                          aCAAAGAGGCCGAGGCGAAATTTAAAGAGATCATGGAAGCTTATGAAGTTCTGACCGACTCGCAAAAACGTGCGGCATACGATCAGTATGGTCATGCTGCGTTTGAGCAAGGTGGCATGGGCGGCGGCGGTTTTggcggc            >  1:7988504/1‑140 (MQ=255)

                                                                                                                          aCAAAGAGGCCGAGGCGAAATTTAAAGAGATCAAGGAAGCTTATGAAGTTCTGACCGACTCGCAAAAACGTGCGGCATACGATCAGTATGGTCAtgct                                                      <  1:1898110/98‑1 (MQ=255)

                                                                                                                                agGCCGAGGCGAAATTTAAAGAGATCAAGGAAGCTTATGAAGTTCTGACCGACTCGCAAAAACGTGc                                                                               <  2:6603532/67‑1 (MQ=255)

                                                                                                                                agGCCGAGGCGAAATTTAAAGAGATCAAGGAAGCTTATGAAGTTCTGACCGACTCGCAAAAACGTGc                                                                               >  1:6603532/1‑67 (MQ=255)

                                                                                                                                agGCCGAGGCGAAATTTAAAGAGATCAAGGAAGCTTATGAAGTTCTGACCGACTCGCAAAAACGTGCGGCATACGATCAGTATGGTCATGCTGCGTTTGAGCAAGGTGGCATGGGCGGCGGCGGTTTTGGCGGCGGCGCa      >  1:1575998/1‑140 (MQ=255)

                                                                                                                                  gCCGAGGCGAAATTTAAAGAGATCAAGGAAGCTTATGAAGTTCTGACCGACTCGCAAAAACGTGCGGCATACGATCAGTATGGTCATGCTGCGTTTGAGCAAGGTGGCATGGGCGGCGGCGGTTTTGGCGGCGGCGCAGa    >  2:2819489/1‑140 (MQ=255)

                                                                                                                                    cGAGGCGAAATTTAAAGAGATCAAGGAAGCTTATGAAGTTCTGACCGACTCGCAAAAACGTGCGGCATAc                                                                        >  2:4130265/1‑70 (MQ=255)

AAAGATGGCTAAGCAAGATTATTACGAGATTTTAGGCGTTTCCAAAACAGCGGAAGAGCGTGAAATCAGAAAGGCCTACAAACGCCTGGCCATGAAATACCACCCGGACCGTAACCAGGGTGACAAAGAGGCCGAGGTGAAATTTAAAGAGATCAAGGAAGCTTATGAAGTTCTGACCGACTCGCAAAAACGTGCGGCATACGATCAGTATGGTCATGCTGCGTTTGAGCAAGGTGGCATGGGCGGCGGCGGTTTTGGCGGCGGCGCAGACT  >  NZ_CP025268/14165‑14436

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`