BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP025268	247,291	A→G	250 (TAA→TAG)	yafL →	C40 family peptidase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP025268	247,291	0	A	G	99.2%	1709.1 / NA	493	250 (TAA→TAG)	yafL	C40 family peptidase
Reads supporting (aligned to +/- strand): ref base A (0/0); major base G (206/283); minor base T (3/1); total (209/284)
Fisher's exact test for biased strand distribution p-value = 3.16e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 493 total aligned reads displayed.

GTGTATTTGGGCGATGGGCAATTTATCGAGTCGCCACGTACCGGCGAAACCATTCGGATAAGCCGATTAGCCGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAAGAC‑G‑ATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACGAGCCCGCGAATATTTGCGAGCCGCTTTCCCGATATAAAACAACCTCATTGCCAA  >  NZ_CP025268/247154‑247425

gtgtATTTGGGCGATGGGCAATTTATCGAGTCGCCACGTACCGGCGAAACCATTCGGATAAGCCGATTAGCCGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAGGa                                                                                                                                        <  1:7328999/140‑1 (MQ=255)

gtgtATTTGGGCGATGGGCAATTTATCGAGTCGCCACGTACCGGCGAAACCATTCGGATAAGCCGATTAGCCGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAGGa                                                                                                                                        <  2:5475305/140‑1 (MQ=255)

gtgtATTAGGGCGATGTGCAATTTATCGAGTCGCCACGTACCGGCGAAACCATTCGGATAATCCGATTAGCCGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGAGCAGGATTTTGACGGAATAGACGATTTTGTAGGa                                                                                                                                        >  2:2959774/1‑140 (MQ=255)

 tgtATTTGGGCGATGGGCAATTTATCGAGTCGCCACGTACCGGCGAAACCATTCGGATAAGCCGATTAGCCGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGACGAGACGATTTTGTAGGAc                                                                                                                                       >  2:8953352/1‑140 (MQ=255)

 tgtATTTGGGCGATGGGCAATTTATCGAGTCGCCACGTACCGGCGAAACCATTCGGATAAGCCGATTAGCCGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAGGAc                                                                                                                                       >  2:3596382/1‑140 (MQ=255)

 tgtATTTGGGCGATGGGCAATTTATCGAGTCGCCACGTACCGGCGAAACCATTCGGATAAGCCGATTAGCCGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAGGAc                                                                                                                                       >  1:6784022/1‑140 (MQ=255)

 tgtATTTGGGCGATGGGCAATTTATCGAGTCGCCACGTACCGGCGAAACCATTCGGATAAGCCGATTAGCCGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAGGAc                                                                                                                                       >  2:6464224/1‑140 (MQ=255)

 tgtATTTGGGCGATGGGCAATTTATCGAGTCGCCACGTACCGGCGAAACCATTCGGATAAGCCGATTAGCCGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAGGAc                                                                                                                                       >  2:5381905/1‑140 (MQ=255)

 tgtATTTGGGCGATGGGCAATTTATCGAGTCGCCACGTACCGGCGAAACCATTCGGATAAGCCGATTAGCCGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAGGAc                                                                                                                                       >  2:7741750/1‑140 (MQ=255)

 tgtATTTGGGCGATGGGCAATTTATCGAGTCGCCACGTACCGGCGAAACCATTCGGATAAGCCGATTAGCCGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAGGAc                                                                                                                                       >  2:3123014/1‑140 (MQ=255)

 tgtATTTGGGCGATGGGCAATTTATCGAGTCGCCACGTACCGGCGAAACCATTCGGATAAGCCGATTAGCCGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAGGAc                                                                                                                                       >  1:1169768/1‑140 (MQ=255)

 tgtATTTGGGCGATGGGCAATTTATCGAGTCGCCACGTACCGGCGAAACCATTCGGATAAGCCGATTAGCCGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAGGAc                                                                                                                                       >  1:1946782/1‑140 (MQ=255)

  gtATTTGGGCGATGGGCAATTTATCGAGTCGCCACGTACCGGCGAAACCATTCGGATAAGCCGATTAGCCGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAGGAC‑g                                                                                                                                     >  2:5745714/1‑140 (MQ=255)

  gtATTTGGGCGATGGGCAATTTATCGAGTCGCCACGTACCGGCGAAACCATTCGGATAAGCCGATTAGCCGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAGGAC‑g                                                                                                                                     >  2:3466636/1‑140 (MQ=255)

  gtATTTGGGCGATGGGCAATTTATCGAGTCGCCACGTACCGGCGAAACCATTCGGATAAGCCGATTAGCCGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAGGAC‑g                                                                                                                                     >  2:6213494/1‑140 (MQ=255)

   tATTTGGGCGATGGGCAATTTATCGAGTCGCCACGTACCGGCGAAACCATTCGGATAAGCCGATTAGCCGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAGGAC‑gg                                                                                                                                    >  1:2698278/1‑139 (MQ=255)

   tATTTGGGCGATGGGCAATTTATCGAGTCGCCACGTACCGGCGAAACCATTCGGATAAGCCGATTAGCCGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATGTTGTAGGAC‑gg                                                                                                                                    >  1:6580605/1‑139 (MQ=255)

     tttGGGCGATGGGCAATTTATCGAGTCGCCACGTACCGGCGAAACCATTCGGATAAGCCGATGAGCCGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAGGAC‑ggat                                                                                                                                  <  1:4683351/140‑4 (MQ=255)

        gggCGATGGGCAATTTATCGAGTCGCCACGTACCGGCGAAACCATTCGGATAAGCCGATTAGCCGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAGGACGG‑ATAAg                                                                                                                               <  2:8051025/140‑1 (MQ=255)

             aTGGGCCATTTATCGAGTCGCCACGTACCGGCGAAACCATTCGGATCAGCCGATTAGCCGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCCGGATTTTGACGGAAGAGACGATTTTGTAGGACGG‑ATAAGGCGtt                                                                                                                          >  2:1649570/1‑140 (MQ=255)

             aTGGGCAATTTATCGAGTCGCCACGTACCGGCGAAACCATTCGGATAAGCCGATTAGCCGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAGGACGG‑ATAAGGCGtt                                                                                                                          >  1:2114573/1‑140 (MQ=255)

             aTGGGCAATTTATCGAGTCGCCACGTACCGGCGAAACCATTCGGATAAGCCGATTAGCCGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAGGACGG‑ATAAGGCGtt                                                                                                                          >  1:6137920/1‑140 (MQ=255)

             aTGGGCAATTTATCGAGTCGCCACGTACCGGCGAAACCATTCGGATAAGCCGATTAGCCGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAGGACGG‑ATAAGGCGtt                                                                                                                          >  2:4197914/1‑140 (MQ=255)

             aTGGGCAATTTATCGAGTCGCCACGTACCGGCGAAACCATTCGGATAAGCCGATTAGCCGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAGGACGG‑ATAAGGCGtt                                                                                                                          >  2:3753575/1‑140 (MQ=255)

             aTGGGCAATTTATCGAGTCGCCACGTACCGGCGAAACCATTCGGATAAGCCGATTAGCCGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAGGACGG‑ATAAGGCGtt                                                                                                                          >  2:8699592/1‑140 (MQ=255)

             aTGGGCAATTTATCGAGTCGCCACGTACCGGCGAAACCATTCGGATAAGCCGATTAGCCGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGAGTTTGTATGACGG‑ATAAGGCGtt                                                                                                                          >  2:2032864/1‑140 (MQ=255)

             aTGGGCAATTTATCGAGTCGCCACGTACCGGCGAAACCATTCGGATAAGCCGATTAGCCGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCACAGGATTTTGACGGAAGAGACGATTTTGTAGGACGG‑ATAAGGCGtt                                                                                                                          >  2:5609037/1‑140 (MQ=255)

             aTGGGCAATTTATCGAGTCGCCACGTACCGGCGAAACCATTCGGATAAGCCGAGTAGCCGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAGGACGG‑ATAAGGCGtt                                                                                                                          >  1:3061111/1‑140 (MQ=255)

               gggCAATTTATCGAGTCGCCACGTACCGGCGAAACCATTCGGATAAGCCGATTAGCCGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAGGACGG‑ATAAGGCGTTTa                                                                                                                        >  1:3397823/1‑140 (MQ=255)

                ggCAATTTATCGAGTCGCCACGTACCGGCGAAACCATTCGGATAAGCCGATTAGCCGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAGGACGG‑ATAAGGCGTTTAc                                                                                                                       <  2:9035703/140‑1 (MQ=255)

                 gCAATTGATCGAGTCGCCACGGACCGGCGACACCAATCGGATAAGCCGATGAGCCGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCAGGCTTTTGACGGAAGAGACGATTTGGTAGGACGG‑ATAAGGCGTTTAcg                                                                                                                      <  2:8135615/140‑1 (MQ=255)

                   aaTTTATCGAGTCGCCACGTACCGGCGAAACCATTCGGATAAGCCGATTAGCCGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAGGACGG‑ATAAGGCGTTTAcgcc                                                                                                                    <  2:1376908/140‑1 (MQ=255)

                    aTTTATCGAGTCGCCACGTACCGGCGAAACCATTCGGATAAGCCGATTAGCCGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAGGACGG‑ATAAGGCGTTTAcgccg                                                                                                                   >  2:4115843/1‑140 (MQ=255)

                      ttATCGAGTCGCCACGTACCGGCGAAACCATTCGGATAAGCCGATTAGCCGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAGGACGG‑ATAAGGCGTTTACGCCGCa                                                                                                                 <  1:5984150/140‑1 (MQ=255)

                           gAGTCGCCACGTACCGGCGAAACCCTGCGGATAAGCCGATTAGCCGAACCTTGCTGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGCTGTTGGAGGACGG‑ATAAGGCGTTTACGCCGCATCCgg                                                                                                            <  2:6662282/140‑1 (MQ=255)

                           gAGTCGCCACGTACCGGCGAAACCATTCGGATAAGCCGATTAGCCGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAGGACGG‑ATAAGGCGTTTACGCCTCATCCgg                                                                                                            <  2:7931688/140‑1 (MQ=255)

                           gAGTCGCCACGTACCGGCGAAACCATTCGGATAAGCCGATTAGCCGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAGGACGG‑ATAAGGCGTTTACGCCGCATCCgg                                                                                                            <  1:6994259/140‑1 (MQ=255)

                           gAGTCGCCACGTACCGGCGAAACCATTCGGATAAGCCGATTAGCCGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAGGACGG‑ATAAGGCGTTTACGCCGCATCCgg                                                                                                            <  1:2455725/140‑1 (MQ=255)

                           gAGTCGCCACGTACCGGCGAAACCATTCGGATAAGCCGATTAGCCGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAGGACGG‑ATAAGGCGTTTACGCCGCATCCgg                                                                                                            <  1:3612666/140‑1 (MQ=255)

                           gAGTCGCCACGTACCGGCGAAACCATTCGGATAAGCCGATTAGCCGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAGGACGG‑ATAAGGCGTTTACGCCGCATCCgg                                                                                                            <  1:8900929/140‑1 (MQ=255)

                           gAGTCGCCACGTACCGGCGAAACCAGTCGGATAAGCCGATTAGCCGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAGGACGG‑ATAAGGCGTTTACGCCGCATCCgg                                                                                                            <  2:8394146/140‑1 (MQ=255)

                              tCGCCACGTACCGGCGAAACCATTCGGATAAGCCGATTAGCCGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAGGACGG‑ATAAGGCGTTTACGCCGCATCCGGCAg                                                                                                         <  1:5266551/140‑1 (MQ=255)

                              tCGCCACGTACCGGCGAAACCATTCGGATAAGCCGATTAGCCGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAGGACGG‑ATAAGGCGTTTACGCCGCATCCGGCAg                                                                                                         <  1:5170558/140‑1 (MQ=255)

                              tCGCCACGTACCGGCGAAACCATTCGGATAAGCCGATTAGCCGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAGGACGG‑ATAAGGCGTTTACGCCGCATCCGGCAg                                                                                                         <  1:126944/140‑1 (MQ=255)

                                gCCACGTACCGGCGAAACCCTTCGGATAAGCCGATTAGCCGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAGGACGG‑ATAAGGCGTTTACGCCGCATCCGGCAGtt                                                                                                       <  1:3956752/140‑1 (MQ=255)

                                     gTACCGGCGAAACCATTCGGATAAGCCGATTAGCCGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAGGACGG‑ATAAGGCGTTTACGCCGCATCCGGCAGTTGTACg                                                                                                  <  1:5854457/140‑1 (MQ=255)

                                       aCCGGCGACACCATTCGGATAAGCCGATTAGCCGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAGGACGG‑ATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCa                                                                                                <  2:3286219/140‑1 (MQ=255)

                                       aCCGGCGAAACCATTCGGATAAGCCGATTAGCCGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAGGACGG‑ATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCa                                                                                                <  1:1089671/140‑1 (MQ=255)

                                        ccGGCGAAACCATTCGGATAAGCCGATTAGCCGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAGGACGG‑ATAAGGCGTTTACGCCGCATCCgg                                                                                                            >  1:6911043/1‑127 (MQ=255)

                                        ccGGCGAAACCATTCGGATAAGCCGATTAGCCGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAGGACGG‑ATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAg                                                                                               >  1:33464/1‑140 (MQ=255)

                                           gCGAAACCATTCGGATAAGCCGATTAGCCGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAGGACGG‑ATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGTg                                                                                            >  2:2143317/1‑140 (MQ=255)

                                           gCGAAACCATTCGGATAAGCCGATTAGCCGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAGGACGG‑ATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGTg                                                                                            >  2:7590225/1‑140 (MQ=255)

                                            cGAAACCATTCGGATAAGCCGATTAGCCGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAGGACGG‑ATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGc                                                                                           >  1:7524563/1‑140 (MQ=255)

                                                aCCATTCGGATAAGCCGATTAGCCGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAGGACGG‑ATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGCCTGa                                                                                       >  2:9373022/1‑140 (MQ=255)

                                                  cATTCGGATAAGCCGATTAGCCGAACCTTTCTGGCATGACCATTTTTTGGGCGCGCGCAGGATTTTTACGGAAGAGACGATTTTGTCGGACGG‑ATAAGGCGTTTACGCCGCATCCGGCATTTGTACGCATGTGCCTGATg                                                                                     <  2:6022689/140‑1 (MQ=38)

                                                  cATTCGGATAAGCCGATTAGCCGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAGGACGG‑ATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGCCTGATg                                                                                     <  1:849365/140‑1 (MQ=255)

                                                   aTTCGGATAAGCCGATTAGCCGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAGGACGG‑ATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGCCTGATGc                                                                                    <  2:2453590/140‑1 (MQ=255)

                                                        gATAAGCCGATTAGCCGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAGGACGG‑ATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGCCTGATGCGACGc                                                                               <  1:7584807/140‑1 (MQ=255)

                                                           aaGCCGATTAGCCGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAGGACGG‑ATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGCCTGATGCGACGCTgg                                                                            <  1:5421074/140‑1 (MQ=255)

                                                            aGCCGATTAGCCGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAGGACGG‑ATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGCCTGATGCGACGCTGgc                                                                           <  1:2635321/140‑1 (MQ=255)

                                                            aGCCGATTAGCCGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAGGACGG‑ATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGCCTGATGCGACGCTGgc                                                                           <  2:7683403/140‑1 (MQ=255)

                                                            aGCCGATTAGCCGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAGGACGG‑ATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGCCTGATGCGACGCTGgc                                                                           <  1:453683/140‑1 (MQ=255)

                                                            aGCCGATTAGCCGAACCTTTCTGGCAGGACCATAGTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAGGACGG‑ATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGCCTGATGCGACGCTGgc                                                                           <  2:7459830/140‑1 (MQ=37)

                                                              ccGATTAGCCGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAGGACGG‑ATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGCCTGATGCGACGCTGgcgc                                                                         >  2:7353914/1‑140 (MQ=255)

                                                                gATTAGCCGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAGGACGG‑ATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGCCTGATGCGACGCTGGCGCGt                                                                       <  2:7189297/140‑1 (MQ=255)

                                                                gATTAGCCGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAGGACGG‑ATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGCCTGATGCGACGCTGGCGCGt                                                                       <  2:6322884/140‑1 (MQ=255)

                                                                  ttAGCCGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAGGACGG‑ATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGCCTGATGCGACGCTGGCGCGTCt                                                                     <  1:998148/140‑1 (MQ=255)

                                                                  ttAGCCGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAGGACGG‑ATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGCCTGATGCGACGCTGGCGCGTCt                                                                     >  2:2745726/1‑140 (MQ=255)

                                                                   tAGCCGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAGGACGG‑ATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGCCTGATGCGACGCTGGCGCGTCtt                                                                    >  1:6371208/1‑140 (MQ=255)

                                                                   tAGCCGAACCTTTCTGGCAGGACCATTTTGTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAGGACGG‑ATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGCCTGATGCGACGCTGGCGCGTCtt                                                                    >  2:5281600/1‑140 (MQ=255)

                                                                     gCCGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAGGACGG‑ATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGCCTGATGCGACGCTGGCGCGTCTTAt                                                                  <  2:5084006/140‑1 (MQ=255)

                                                                      ccGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAGGACGG‑ATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGCCTGATGCGACGCTGGCGCGTCTTATc                                                                 <  2:4009195/140‑1 (MQ=255)

                                                                      cagAACCTTTCTGGCAGGACCATTTTTTGTGCGCGCGCAGGAATTTGACGGACGAGACGATTTTGAAGGAC‑GCATAACGCGATTACGCCGCATCCGGCAGTTGGACGCAGGTGCCTGATGCGACGCTGGCGCTTCTTATc                                                                 <  2:6522535/138‑1 (MQ=25)

                                                                        gAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAGGACGG‑ATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGCCTGATGCGACGCTGGCGCGTCTTATCAt                                                               <  2:3097161/140‑1 (MQ=255)

                                                                        gAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAGGACGG‑ATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGCCTGATGCGACGCTGGCGCGTCTTATCAt                                                               <  1:8155349/140‑1 (MQ=255)

                                                                               tCTGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAGGACGG‑ATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACg                                                        <  1:8736455/140‑1 (MQ=255)

                                                                                    cAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAGGACGG‑ATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACGAGccc                                                   <  1:3224519/140‑1 (MQ=255)

                                                                                    cAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAGGACGG‑ATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACGAGccc                                                   <  2:4799947/140‑1 (MQ=255)

                                                                                      ggACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAGGACGG‑ATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACGAGCCcgc                                                 >  2:2877112/1‑140 (MQ=255)

                                                                                      ggACCATGTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAGGACGG‑ATACGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACGAGCCcgc                                                 >  2:9177410/1‑140 (MQ=25)

                                                                                                tagggCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAGGACGG‑ATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGCCTGATGCGACGCTGGCGCGTATTATCATGCCTACGAGCCCGCGAATAATTGc                                       <  2:6925491/138‑1 (MQ=25)

                                                                                                 tGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAGGACGG‑ATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACGAGCCCGCGAATATTTGCg                                      <  1:49960/140‑1 (MQ=255)

                                                                                                  gggCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAGGACGG‑ATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACGAGCCCGCGAATATTTGCGa                                     <  2:2300545/140‑1 (MQ=255)

                                                                                                   ggCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAGGACGG‑ATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACGAGCCCGCGAATATTTGCGAg                                    <  1:9319294/140‑1 (MQ=255)

                                                                                                       cgcgcAGGATTTTGACGGAAGAGACGATTTTGTAGGACGG‑ATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACGAGCCCGCGAATATTTGCGAGCCGc                                <  2:1293438/140‑1 (MQ=255)

                                                                                                       cgcgcAGGATTTTGACGGAAGAGACGATTTTGTAGGACGG‑ATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACGAGCCCGCGAATATTTGCGAGCCGc                                >  2:780528/1‑140 (MQ=255)

                                                                                                       cgcgcAGGATTTTGACGGAAGAGACGATTTTGTAGGACGG‑ATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGCCTGATGCGACGCTGGCGCGTCTTATAATGCCTACGAGCCCGCGAATATTTGCGAGCCGc                                >  1:7733834/1‑140 (MQ=255)

                                                                                                         cgcAGGATTTTGACGGAAGAGACGATTTTGTAGGACGG‑ATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACGAGCCCGCGAATATTTGCGAGCCGCtt                              >  1:5672862/1‑140 (MQ=255)

                                                                                                            aGGATTTTGACGGAAGAGACGATTTTGTAGGACGG‑ATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACGAGCCCGCGAATATTTGCGAGCCGCTTTcc                           <  1:9070045/140‑1 (MQ=255)

                                                                                                            aGGATTTTGACGGAAGAGACGATTTTGTAGGACGG‑ATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACGAGCCCGCGAATATTTGCGAGCCGCTTTcc                           <  2:4510476/140‑1 (MQ=255)

                                                                                                            aGGATTTTGACGGAAGAGACGATTTTGTAGGACGG‑ATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACGAGCCCGCGAATATTTGCGAGCCGCTTTcc                           <  1:4100569/140‑1 (MQ=255)

                                                                                                            aGGATTTTGACGGAAGAGACGATTTTGTAGGACGG‑ATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACGAGCCCGCGAATATTTGCGAGCCGCTTTcc                           <  1:1474899/140‑1 (MQ=255)

                                                                                                            aGGATTTTGACGGAAGAGACGATTTTGTAGGACGG‑ATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACGAGCCCGCGAATATTTGCGAGCCGCTTTcc                           <  1:7980577/140‑1 (MQ=255)

                                                                                                                  ttGACGGAAGAGACGATTTTGTAGGACGG‑ATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACGAGCCCGCGAATATTTGCGAGCCGCTTTCCCGatat                     <  2:1105425/140‑1 (MQ=255)

                                                                                                                        gAAGAGACGATTTTGTAGGACGG‑ATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACGAGCCCGCGAATATTTGCGAGCCGCTTTCCCGATATAAaaca               >  1:8370413/1‑140 (MQ=255)

                                                                                                                        gAAGAGACGATTTTGAAGGACGG‑ATAATGCGTTTACGCCGCAACCGGCAGTTGTACGCAGGTGCCTGATGCGACGCTGGCGCGTCTTATCATGACTACGAGCCCGCGAATCTTTGCGAGCCGCTTTCCCGATATAAaaca               >  2:1574862/1‑140 (MQ=25)

                                                                                                                           gagaCGATTTTGTAGGACGG‑ATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACGAGCCCGCGAATATTTGCGAGCCGCTTTCCCGATATAAAACAAcc            <  1:1630106/140‑1 (MQ=255)

                                                                                                                            agaCGATTTTGTAGGACGG‑ATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACGAGCCCGCGAATATTTGCGAGCCGCTTTCCCGATATAAAACAACCt           <  1:2932005/140‑1 (MQ=255)

                                                                                                                                     tGTAGGACGG‑ATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACGAGCCCGCGAATATTTGCGAGCCGCTTTCCCGATATAAAACAACCTCATTGcc    <  2:2543450/138‑1 (MQ=255)

                                                                                                                                     tGTAGGAC‑GTATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACGAGCCCGCGAATATTTGCGAGCCGCTTTCCCGATATAAAACAACCTCATTGCCaa  >  1:1316974/1‑140 (MQ=255)

GTGTATTTGGGCGATGGGCAATTTATCGAGTCGCCACGTACCGGCGAAACCATTCGGATAAGCCGATTAGCCGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAAGAC‑G‑ATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACGAGCCCGCGAATATTTGCGAGCCGCTTTCCCGATATAAAACAACCTCATTGCCAA  >  NZ_CP025268/247154‑247425

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`