Predicted mutation
evidence seq id position mutation annotation gene description
RA NZ_CP025268 2,773,510 A→G *274* (TAA→TAG)  yfjQ → DUF932 domain‑containing protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0252682,773,5100AG99.8% 2090.0 / NA 603*274* (TAA→TAG) yfjQDUF932 domain‑containing protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  major base G (313/289);  minor base T (1/0);  total (314/289)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01

Only 100 of 603 total aligned reads displayed.

ATTAAGGGCGGACTCAGTGGGCGTAATGCTAAAGGCGGACGAACTCATACTCGTGCCGTGCGTGGCATTGACGGGGATGTGAAGCTTAACCGTGCGCTGTGGGTGATGGCAGAAACACTGCTCACGCAACTGCAGTAAACGTTTCATGTTGCCACGTTGTTAATATCGGACACCACCTGTCCGCATCGCTATGTGCTCGTGTGCCTCAATCCCCCGGTTATAGCTTTTAACCCCCGTTACATCTGGCTTTTGCAGAAATAAAAAATAGTTTCT  >  NZ_CP025268/2773373‑2773645
                                                                                                                                         |                                                                                                                                       
      ggCGGACTCAGTGGGCGTAATGCTAAAGGCGGACGAACTCATACTCGTGCCGTGCGTGGCATTGACGGGGATGTGAAGCTTAACCGTGCGCTGTGGGTGATGGCAGAAACACTGCTCACGCAACTGCAGTAGACGTTTCa                                                                                                                                 >  2:1028262/1‑140 (MQ=255)
          gACTCAGTGGGCGTAATGCTAAAGGCGGACGAACTCATACTCGTGCCGTGCGTGGCATTGACGGGGATGTGAAGCTTAACCGTGCGCTGTGGGTGATGGCAGAAACACTGCTCACGCAACTGCAGTAGACGTTTCATGtt                                                                                                                             >  1:4858619/1‑140 (MQ=255)
          gACTCAGTGGGCGTAATGCTAAAGGCGGACGAACTCATACTCGTGCCGTGCGTGGCATTGACGGGGATGTGAAGCTTAACCGTGCGCTGTGGGTGATGGCAGAAACACTGCTCACGCAACTGCAGTAGACGTGTCATGtt                                                                                                                             >  2:5173071/1‑140 (MQ=255)
          gACTCAGTGGGCGTAATGCTAAAGGCGGACGAACTCATACTCGTGCCGAGCGTGGCATTGACGGGGATGTGAAGCTAAACCGTGCGCTGTGGGTGATGGCAGAAACACTGCTCACGCACCTGCAGTAGACGTTTCATGtt                                                                                                                             >  2:4023517/1‑140 (MQ=255)
           aCTCAGTGGGCGTAATGCTAAAGGCGGACGAACTCATACTCGTGCCGTGCGTGGCATTGACGGGGATGTGAAGCTTAACCGTGCGCTGTGGGTGATGGCAGAAACACTGCTCACGCAACTGCAGTAGACGTTTCATGTTg                                                                                                                            <  1:1618245/140‑1 (MQ=255)
             tCAGTGGGCGTAATGCTAAAGGCGGACGAACTCATACTCGTGCCGTGCGTGGCATTGACGGGGATGTGAAGCTTAACCGTGCGCTGTGGGTGATGGCAGAAACACTGCTCACGCAACTGCAGTAGACGTTTCATGTTGcc                                                                                                                          <  1:545431/140‑1 (MQ=255)
             tCAGTGGGCGTAATGCTAAAGGCGGACGAACTCATACTCGTGCCGTGCGTGGCATTGACGGGGATGTGAAGCTTAACCGTGCGCTGTGGGTGATGGCAGAAACACTGCTCACGCAACTGCAGTAGACGTTTCATGTTGcc                                                                                                                          <  2:5297363/140‑1 (MQ=255)
             tCAGTGGGCGTAATGCTAAAGGCGGACGAACTCATACTCGTGCCGTGCGTGGCATTGACGGGGATGTGAAGCTTAACCGTGCGCTGTGGGTGATGGCAGAAACACTGCTCACGCAACTGCAGTAGACGTTTCATGTTGcc                                                                                                                          <  2:433738/140‑1 (MQ=255)
              cAGTGGGCGTAATGCTAAAGGCGGACGAACTCATACTCGTGCCGTGCGTGGCATTGACGGGGATGTGAAGCTTAACCGTGCGCTGTGGGTGATGGCAGAAACACTGCTCACGCAACTGCAGTAGACGTTTCATGTTGCCa                                                                                                                         <  1:881605/140‑1 (MQ=255)
               aGTGGGCGTAATGCTAAAGGCGGACGAACTCATACTCGTGCCGTGCGTGGCATTGACGGGGATGTGAAGCTTAACCGTGCGCTGTGGGTGATGGCAGAAACACTGCTCACGCAACTGCAGTAGACGTTTCATGTTGCCAc                                                                                                                        <  2:9141204/140‑1 (MQ=255)
               aGTGGGCGTAATGCTAAAGGCGGACGAACTCATACTCGTGCCGTGCGTGGCATTGACGGGGATGTGAAGCTTAACCGTGCGCTGTGGGTGATGGCAGAAACACTGCTCACGCAACTGCAGTAGACGTTTCATGTTGCCAc                                                                                                                        <  1:7078093/140‑1 (MQ=255)
               aGTGGGCGTAATGCTAAAGGCGGACGAACTCATACTCGTGCCGTGCGTGGCATTGACGGGGATGTGAAGCTTAACCGTGCGCTGTGGGTGATGGCAGAAACACTGCTCACGCAACTGCAGTAGACGTTTCATGTTGCCAc                                                                                                                        <  1:2758216/140‑1 (MQ=255)
                gTGGGCGTAATGCTAAAGGCGGACGAACTCATACTCGTGCCGTGCGTGGCATTGACGGGGATGTGAAGCTTAACCGTGCGCTGTGGGTGATGGCAGAAACACTGCTCACGCAACTGCAGTAGACGTTTCATGTTGCCACg                                                                                                                       <  2:8893706/140‑1 (MQ=255)
                gTGGGCGTAATGCTAAAGGCGGACGAACTCATACTCGTGCCGTGCGTGGCATTGACGGGGATGTGAAGCTTAACCGTGCGCTGTGGGTGATGGCAGAAACACTGCTCACGCAACTGCAGTAGACGTTTCATGTTGCCACg                                                                                                                       <  1:567403/140‑1 (MQ=255)
                  gggCGTAATGCTAAAGGCGGACGAACTCATACTCGTGCCGTGCGTGGCATTGACGGGGATGTGAAGCTTAACCGTGCGCTGTGGGTGATGGCAGAAACACTGCTCACGCAACTGCAGTAGACGTTTCATGTTGCCACgtt                                                                                                                     >  2:3320006/1‑140 (MQ=255)
                    gCGTAATGCTAAAGGCGGACGAACTCATACTCGTGCCGTGCGTGGCATTGACGGGGATGTGAAGCTTAACCGTGCGCTGTGGGTGATGGCAGAAACACTGCTCACGCAACTGCAGTAGACGTTTCATGTTGCCACgttgt                                                                                                                   <  2:6203830/140‑1 (MQ=255)
                    gCGTAATGCTAAAGGCGGACGAACTCATACTCGTGCCGTGCGTGGCATTGACGGGGATGTGAAGCTTAACCGTGCGCTGTGGGTGATGGCAGAAACACTGCTCACGCAACTGCAGTAGACGTTTCATGTTGCCACgttgt                                                                                                                   <  1:8642547/140‑1 (MQ=255)
                     cGTAATGCTAAAGGCGGACGAACTCATACTCGTGCCGTGCGTGGCATTGACGGGGATGTGAAGCTTAACCGTGCGCTGTGGGTGATGGCAGAAACACTGCTCACGCAACTGCAGTAGACGTTTCATGTTGCCACgttgtt                                                                                                                  >  1:8452923/1‑140 (MQ=255)
                     cGTAATGCTAAAGGCGGACGAACTCATACTCGTGCCGTGCGTGGCATTGACGGGGATGTGAAGCTTAACCGTGCGCTGTGGGTGATGGCAGAAACACTGCTCACGCAACTGCAGTAGACGTTTCATGTTGCCACgttgtt                                                                                                                  >  2:5734395/1‑140 (MQ=255)
                      gTAATGCTAAAGGCGGACGAACTCATACTCGTGCCGTGCGTGGCATTGACGGGGATGTGAAGCTTAACCGTGCGCTGTGGGTGATGGCAGAAACACTGCTCACGCAACTGCAGTAGACGTTTCATGTTGCCACGTTGTTa                                                                                                                 <  1:4285525/140‑1 (MQ=255)
                         aTGCTAAAGGCGGACGAACTCATACTCGTGCCGTGCGTGTCATTGACGGGGATGTGAAGCTTAACCGTGCGCAGTGGGTGATTGCAGAAACACTGCTCACGCAACTGCAGTAGACGTTTAATTTTGCCACGTAGTTAata                                                                                                              >  2:1907789/1‑140 (MQ=25)
                         aTGCTAAAGGCGGACGAACTCATACTCGTGCCGTGCGTGGCATTGACGGGGATGTGAAGCTTAACCGTGCGCTGTGGGTGATGGCAGAAACACTGCTCACGCAACTGCAGTAGACGTTTCATGTTGCCACg                                                                                                                       >  2:3454667/1‑131 (MQ=255)
                         aTGCTAAAGGCGGACGAACTCATACTCGTGCCGTGCGTGGCATTGACGGGGATGTGAAGCTTAACCGTGCGCTGTGGGTGATGGCAGAAACACTGCTCACGCAACTGCAGTAGACGTTTCATGTTGCCACg                                                                                                                       <  1:3453667/131‑1 (MQ=255)
                         aTGCTAAAGGCGGACGAACTCATACTCGTGCCGTGCGTGGCATTGACGGGGATGTGAAGCTTAACCGTGCGCTGTGGGTGATGGCAGAAACACTGCTCACGCAACTGCAGTAGACGTTTCATGTTGCCACGTTGTTAata                                                                                                              >  2:1420363/1‑140 (MQ=255)
                            cTAAAGGCGGACGAACTCATACTCGTGCCGTGCGTGGCATTGACGGGGATGTGAAGCTTAACCGTGCGCTGTGGGTGATGGCAGAAACACTGCTCACGCAACTGCAGTAGACGTTTCATGTTGCCACGTTGTTAATATCg                                                                                                           <  1:925718/140‑1 (MQ=255)
                              aaaGGCGGACGAACTCATACTCGTGCCGTGCGTGGCATTGACGGGGATGTGAAGCTTAACCGTGCGCTGTGGGTGATGGCAGAAACACTGCTCACGCAACTGCAGTAGACGTTTCATGTTGCCACGTTGTTAATATCGGa                                                                                                         >  1:9015438/1‑140 (MQ=255)
                               aaGGCGGACGAACTCATACTCGTGCCGTGCGTGGCATTGACGGGGATGTGAAGCTTAACCGTGCGCTGTGGGTGATGGCAGAAACACTGCTCACGCAACTGCAGTAGACGTTTCATGTTGCCACGTTGTTAATATCGGac                                                                                                        >  1:2289058/1‑140 (MQ=255)
                                     gACGAACTCATACTCGTGCCGTGCGTGGCATTGACGGGGATGTGAAGCTTAACCGTGCGCTGTGGGTGATGGCAGAAACACTGCTCACGCAACTGCAGTAGACGTTTCATGTTGCCACGTTGTTAATATCGGACaccacc                                                                                                  >  1:2559004/1‑140 (MQ=255)
                                      aCGAACTCATACTCGTGCCGTGCGTGGCATTGACGGGGATGTGAAGCTTAACCGTGCGCTGTGGGTGATGGCAGAAACACTGCTCACGCAACTGCAGTAGACGTTTCATGTTGCCACGTTGTTAATATCGGACACCACCt                                                                                                 <  1:5130798/140‑1 (MQ=255)
                                        gaccTCATACTCGTGCCGAGCGTGGCATTGCCGGGGATGTGAAGCTTAACCGTGCGCTGTGGGTGATGGCAGAAACACTGCTCACGCAACTGCAGTAGACGTTTCATGTTGCCACGTTGTTAATATCGGACACCACCTGt                                                                                               <  2:2372878/137‑1 (MQ=255)
                                           cTCATACTCGTGCCGTGCGTGGCATTGACGGGGATGTGAAGCTTAACCGTGCGCTGTGGGTGATGGCAGAAACACTGCTCACGCAACTGCAGTAGACGTTTCATGTTGCCACGTTGTTAATATCGGACACCACCTGTCCg                                                                                            <  1:4028565/140‑1 (MQ=255)
                                           cTCATACTCGTGCCGTGCGTGGCATTGACGGGGATGTGAAGCTTAACCGTGCGCTGTGGGTGATGGCAGAAACACTGCTCACGCAACTGCAGTAGACGTTTCATGTTGCCACGTTGTTAATATCGGACACCACCTGTCCg                                                                                            >  1:3006934/1‑140 (MQ=255)
                                             cATACTCGTGCCGTGCGTGGCATTGACGGGGATGTGAAGCTTAACCGTGCGCTGTGGGTGATGGCAGAAACACTGCTCACGCAACTGCAGTAGACGTTTCATGTTGCCACGTTGTTAATATCGGACACCACCTGTCCGCa                                                                                          <  1:7387432/140‑1 (MQ=255)
                                                  tCGTGCCGTGCGTGGCATGGCCGGGGATGTGACGCTTAACCGTGCGCTGTGGGTGATGGCAGAAACACTGCTCACGCAACTGCAGTAGACGTTTCATGTTGCCACGTTGTTACTATCGGACACCACCTGTCCGCATCGCt                                                                                     <  2:565752/140‑1 (MQ=21)
                                                    gtgccgtgcGGGGCATTGACGGGGATGTGAAGCTTAACAGTGCGCGGTGGGTGATGGCAGAAACACTGCTCACGCACCTGCAGTTGACGTGTCATGTTTCCACGTTGTTAAGCTCGGACACCCCCGGTCCGCAGCGCTAt                                                                                   >  2:7344107/1‑140 (MQ=255)
                                                     tgccgtgcGTGGCATTGACGGGGATGTGAAGCTTAACCGTGCGCTGTGGGTGATGGCAGAAACACTGCTCACGCACCTGCAGTAGACGTTTCATGTTGCCACGTTGTTAATATCGGACACAACCTGTCCGCATCGCTCtg                                                                                  >  2:7637941/1‑140 (MQ=255)
                                                     tgccgtgcGTGGCATTGACGGGGATGTGAAGCTTAACCGTGCGCTGTGGGTGATGGCAGAAACACTGCTCACGCAACTGCAGTAGACGTTTCATGTTGCCACGTTGTTAATATCGGACACCACCTGTCCGCATCGCTAtg                                                                                  >  1:3292284/1‑140 (MQ=255)
                                                      gccgtgcGTGGCATTGACGGGGATGTGAAGCTTAACCGTGCGCTGTGGGTGATGGCAGAAACACTGCTCACGCAACTGCAGTAGACGTTTCATGTTGCCACGTTGTTAATATCGGACACCACCTGTCCGCATCGCTAtgt                                                                                 >  2:8571610/1‑140 (MQ=255)
                                                      gccgtgcGTGGCATTGACGGGGATGTGAAGCTTAACCGTGCGCTGTGGGTGATGGCAGAAACACTGCTCACGCAACTGCAGTAGACGTTTCATGTTGCCACGTTGTTAATATCGGACACCACCTGTCCGCATCGCTAtgt                                                                                 >  1:5882563/1‑140 (MQ=255)
                                                      gccgtgcGTGGCATTGACGGGGATGTGAAGCTTAACCGTGCGCTGTGGGTGATGGCAGAAACACTGCTCACGCAACTGCAGTAGACGTTTCATGTTGCCACGTTGTTAATATCGGACACCACCTGTCCGCATCGCTAtgt                                                                                 <  1:1896939/140‑1 (MQ=255)
                                                      gccgtgcGTGGCATTGACGGGGATGTGAAGCTTAACCGTGCGCTGTGGGTGATGGCAGAAACACTGCTCACGCAACTGCAGTAGACGTTTCATGTTGCCACGTTGTTAATATCGGACACCACCTGTCCGCATCGCTAtgt                                                                                 <  2:3741722/140‑1 (MQ=255)
                                                      gccgtgcGTGGCATTGACGGGGATGTGAAGCTTAACCGTGCGCTGTGGGTGATGGCAGAAACACTGCTCACGCAACTGCAGTAGACGTTTCATGTTGCCACGTTGTTAATATCGGACACCACCTGTCCGCATCGCTAtgt                                                                                 <  2:8280852/140‑1 (MQ=255)
                                                       ccgtgcGTGGCATTGACGGGGATGTGAAGCTTAACCGTGCGCTGTGGGTGATGGCAGAAACACTGCTCACGCAACTGCAGTAGACGTTTCATGTTGCCACGTTGTTAATATCGGACACCACCTGTCCGCATCGCTAtgtg                                                                                <  2:2752906/140‑1 (MQ=255)
                                                        cgtgcgtgGCATTGACGGGGATGTGAAGCTTAACCGTGCGCTGTGGGTGATGGCAGAAACACTGCTCACGCAACTGCAGTAGACGTTTCATGTTGCCACGTTGTTAATATCGGac                                                                                                        >  1:8318490/1‑115 (MQ=255)
                                                        cgtgcgtgGCATTGACGGGGATGTGAAGCTTAACCGTGCGCTGTGGGTGATGGCAGAAACACTGCTCACGCAACTGCAGTAGACGTTTCATGTTGCCACGTTGTTAATATCGGACACCACCTGTCCGCATCGCTATGTGc                                                                               >  1:3952980/1‑140 (MQ=255)
                                                          tgcgtgGCATTGACGGGGATGTGAAGCTTAACCGTGCGCTGTGGGTGATGGCAGAAACACTGCTCACGCAACTGCAGTAGACGTTTCATGTTGcc                                                                                                                          >  1:52658/1‑95 (MQ=255)
                                                          tgcgtgGCATTGACGGGGATGTGAAGCTTAACCGTGCGCTGTGGGTGATGGCAGAAACACTGCTCACGCAACTGCAGTAGACGTTTCATGTTGCCACGTTGTTAATATCGGNCACCACCTGTCCGCATCGCTATGTGCTc                                                                             >  1:6252319/1‑140 (MQ=255)
                                                          tgcgtgGCATTGACGGGGATGTGAAGCTTAACCGTGCGCTGTGGGTGATGGCAGAAACACTGCTCACGCAACTGCAGTAGACGTTTCATGTTGCCACGTTGTTAATATCGGACACCACCTGTCCGCATCGCTATGTGCTc                                                                             >  1:8042205/1‑140 (MQ=255)
                                                           gcgtgGCATTGACGGGGATGTGAAGCTTAACCGTGCGCTGTGGGTGATGGCAGAAACACTGCTCACGCAACTGCAGTAGACGTTTCATGTTGCCACGTTGTTAATATCGGACACCACCTGTCCGCATCGCTATGTGCTCg                                                                            <  2:6324118/140‑1 (MQ=255)
                                                             gtgGCATTGACGGGGATGTGAAGCTTAACCGTGCGCTGTGGGTGATGGCAGAAACACTGCTCACGCAACTGCAGTAGACGTTTCATGTTGCCACGTTGTTAATATCGGACACCACCTGTCCGCATCGCTATGTGCTCgtg                                                                          <  1:6925321/140‑1 (MQ=255)
                                                              tgGCATTGACGGGGATGTGAAGCTTAACCGTGCGCTGTGGGTGATGGCAGAAACACTGCTCACGCAACTGCAGTAGACGTTTCATGTTGCCACGTTGTTAATATCGGACACCACCTGTCCGCATCGCTATGTGCTCgtgt                                                                         >  2:8796662/1‑140 (MQ=255)
                                                                gCATTGACGGGGATGTGAAGCTTAACCGTGCGCTGTGGGTGATGGCAGAAACACTGCTCACGCAACTGCAGTAGACGTTTCATGTTGCCACGTTGTTAATATCGGACACCACCTGTCCGCATCGCTATGTGCTCGTGTGc                                                                       >  1:35315/1‑140 (MQ=255)
                                                                  aTTGACGGGGATGTGAAGCTTAACCGTGCGCTGTGGGTGATGGCAGAAACACTGCTCACGCAACTGCAGTAGACGTTTCATGTTGCCACGTTGTTAATATCGGACACCACCTGTCCGCATCGCTATGTGCTCGTGTGCCt                                                                     <  1:7659060/140‑1 (MQ=255)
                                                                    tGACGGGGATGTGAAGCTTAACCGTGCGCTGTGGGTGATGGCAGAAACACTGCTCACGCAACTGCAGTAGACGTTTCATGTTGCCACGTTGTTAATATCGGACACCACCTGTCCGCATCGCTATGTGCTCGTGTGCCTCa                                                                   <  2:7797510/140‑1 (MQ=255)
                                                                    ggccGGGGCTGTGAAGCTTAACCGTGCGCTGTGGGAGAGGGCAGAAACCCTGCTCACGCACCTGCAGTAGACGTTTCAGGTTGCCACGGTGTTAAAATCGGACACCCCCTGTCCGCATCGCTATGTGCTCGTGTGCCTCa                                                                   <  2:8079284/137‑1 (MQ=255)
                                                                         gggATGTGAAGCTTAACCGTGCGCTGTGGGTGATGGCAGAAACACTGCTCACGCAACTGCAGTAGACGTTTCATGTTGCCACGTTGTTAATATCGGACACCACCTGTCCGCATCGCTATGTGCTCGTGTGCCTCAATccc                                                              <  1:1310622/140‑1 (MQ=255)
                                                                           gATGTGAAGCTTAACCGTGCGCTGTGGGTGATGGCAGAAACACTGCTCACGCAACTGCAGTAGACGTTTCATGTTGCCACGTTGTTAATATCGGACACCACCTGTCCGCATCGCTATGTGCTCGTGTGCCTCAATccccc                                                            >  2:178276/1‑140 (MQ=255)
                                                                             tgtgAAGCTTAACCGTGCGCTGTGGGTGATGGCAGAACCACTGCTCACGCCACTGCAGTAGACGTTTCATGTTGCCACGTTGGTAATATCGGACACCACCTGTCCGCATCGCTATGTGCTCGTGTGCCTCAATCCCCCgg                                                          >  2:722712/1‑140 (MQ=255)
                                                                               tgAAGCTTAACCGTGCGCTGTGGGTGATGGCAGAAACACTGCTCACGCAACTGCAGTAGACGTTTCATGTTGCCACGTTGTTAATATCGGACACCACCTGTCCGCATCGCTATGTGCTCGTGTGCCTCAATCCCCCGGtt                                                        >  2:2640321/1‑140 (MQ=255)
                                                                                 aaGCTTACCCGTGCGCTGTGGGTGATGGCAGAAACACTGCTCACGCAACTGCAGTAGACGTTTCATGTTGCCACGTTGTTAATATCGGACACCACCTGTCCGCATCGCTATGTGCTCGTGTGCCTCAATCCCCCGGTtat                                                      <  2:3087439/140‑1 (MQ=255)
                                                                                 aaGCTTAACCGTGCGCTGTGGGTGATGGCAGAAACACTGCTCACGCAACTGCAGTAGACGTTTCATGTTGCCACGTTGTTAATATCGGACACCACCTGTCCGCATCGCTATGTGCTCGTGTGCCTCAATCCCCCGGTtat                                                      >  1:1681978/1‑140 (MQ=255)
                                                                                    cTTAACCGTGCGCTGTGGGTGATGGCAGAAACACTGCTCACGCAACTGCAGTAGACGTTTCATGTTGCGACGTTGGTAATATCGGACGCCACCTGTCCGCATCGCTATGTGCTCGTGTGCCTCAGTCCCCCGGTGATAGc                                                   >  2:4799621/1‑140 (MQ=25)
                                                                                       aaCCGTGCGCTGTGGGTGATGGCAGAAACACTGCTCACGCAACTGCAGTAGACGTTTCATGTTGCCACGTTGTTAATATCGGACACCACCTGTCCGCATCGCTCTGTGCTCGTGTGCCTCAATCCCCCGGTTATTGCttt                                                >  1:6816775/1‑140 (MQ=255)
                                                                                         ccGTGCGCTGTGGGTGATGGCAGAAACACTGCTCACGCAACTGCAGTAGACGTTTCATGTTGCCACGTTGTTAATATCGGACACCACCTGTCCGCATCGCTCTGTGCTCGTGTGCCTCAATCCCCCGGTTATAGCTTTTa                                              >  2:5991663/1‑140 (MQ=255)
                                                                                           gTGCGCTGTGGGTGATGGCAGAAACACTGCTCACGCAACTGCAGTAGACGTTTCATGTTGCCACGTTGTTAATATCGGACACCACCTGTCCGCATCGCTATGTGCTCGTGTGCCTCATTCCCCCGGTGCTAGCTGTTAAc                                            >  2:6751428/1‑140 (MQ=25)
                                                                                           gTGCGCTGTGGGTGATGGCAGAAACACTGCTCACGCAACTGCAGTAGACGTTTCATGTTGCCACGTTGTTAATATCGGACACCACCTGTCCGCATCGCTATGTGCTCGTGTGCCTCAATCCCCCGGTTATAGCtttttag                                            >  1:6593583/1‑137 (MQ=255)
                                                                                           gTGCGCTGTGGGTGATGGCAGAAACACTGCTCACGCAACTGCAGTAGACGTTTCATGTTGCCACGTTGTTAATATCGGACACCACCTGTCCGCATCGCTATGTGCTCGTGTGCCTCAATCCCCCGGTTATAGCTTTTAAc                                            >  2:2897412/1‑140 (MQ=255)
                                                                                           gTGCGCTGTGGGTGATGGCAGAAACACTGCTCACGCAACTGCAGTAGACGTTTCATGTTGCCACGTTGTTAATATCGGACACCACCTGTCCGCATCGCTATGTGCTCGTGTGCCTCAATCCCCCGGTTATAGCTTTTAAc                                            <  1:9089193/140‑1 (MQ=255)
                                                                                           gTGCGCTGTGGGTGATGGCAGAAACACTGCTCACGCAACTGCAGTAGACGTTTCATGTTGCCACGTTGTTAATATCGGACACCACCTGTCCGCATCGCTATGTGCTCGTGTGCCGCAATCCCCCGGTTATAGCTTTTAAc                                            >  2:6588147/1‑140 (MQ=255)
                                                                                            tGCGCTGTGGGTGATGGCAGAAACACTGCTCACGCAACTGCAGTAGACGTTTCATGTTGCCACGTTGTTAATATCGGACACCACCTGTCCGCATCGCTATGTGCTCGTGTGCCTCAATCCCCCGGTTATAGCTTTTAAcc                                           <  2:3174991/140‑1 (MQ=255)
                                                                                            tGCGCTGTGGGTGATGGCAGAAACACTGCTCACGCAACTGCAGTAGACGTTTCATGTTGCCACGTTGTTAATATCGGACACCACCTGTCCGCATCGCTATGTGCTCGTGTGCCTCAATCCCCCGGTTATAGCTTTTAAcc                                           >  1:2676587/1‑140 (MQ=255)
                                                                                            tGCGCTGTGGGTGATGGCAGAAACACTGCTCACGCAACTGCAGTAGACGTTTCATGTTGCCACGTTGTTAATATCGGACACCACCTGTCCGCATCGCTATGTGCTCGTGTGCCTCAATCCCCCGGTTATAGCTTTTAAcc                                           <  1:4527044/140‑1 (MQ=255)
                                                                                                cTGTGGGTGATGGCAGAAACACTGCTCACGCAACTGCAGTAGACGTTTCATGTTGCCACGTTGTTAATATCGGACACCACCTGTCCGCATCGCTATGTGCTCGTGTGCCTCAATCCCCCGGTTATAGCTTTTAACCCCCg                                       <  2:6440621/140‑1 (MQ=255)
                                                                                                   tgGGTGATGGCAGAAACACTGCTCACGCAACTGCAGTAGACGTTTCATGTTGCCACGTTGTTAATATCGGACACCACCTGTCCGCATCGCTATGTGCTCGTGTGCCTCAATCCCCCGGTTATAGCTTTTCTCCCCCGTTa                                    >  1:6700867/1‑140 (MQ=255)
                                                                                                    gggTGATGGCAGAAACACTGCTCACGCAACTGCAGTAGACGTTTCATGTTGCCACGTTGTTAATATCGGACACCACCTGTCCGCATCGCTATGTGCTCGTGTGCCTCAATCCCCCGGGTATAGCTTTTAACCCCCGTGAc                                   >  2:8456924/1‑140 (MQ=255)
                                                                                                      gTGATGGCAGAAACACTGCTCACGCAACTGCAGTCGACGTTTCATGTTGCCACGTTGTTAATATCGGACACCACCTGTCCGCATCGCTATGTGCTCGTGTGCCTCATTCCCCCGGTTATCGCGGTTTACCCCCggttcgt                                 >  2:7035425/1‑134 (MQ=25)
                                                                                                      gTGATGGCAGAAACACTGCTCACGCAACTGCAGTAGACGTTTCATGTTGCCACGTTGTTAATATCGGACACCACCTGTCCGCATCGCTATGTGCTCGTGTGCCTCAATCCCCCGGTTATAGCTTTTAACCCCCGTTACAt                                 <  1:1984076/140‑1 (MQ=255)
                                                                                                         aTGGCAGAAACACTGCTCACGCAACTGCAGTAGACGTTTCATGTTGCCACGTTGTTAATATCGGACACCACCTGTCCGCATCGCTATGTGCTCGTGTGCCTCAATCCCCCGGTTATTGCTTTTATCCCCCGTTACATCTg                              >  1:374937/1‑140 (MQ=255)
                                                                                                         aTGGCAGAAACACTGCTCACGCAACTGCAGTAGACGTTTCATGTTGCCACGTTGTTAATATCGGACACCACCTGTCCGCATCGCTATGTGCTCGTGTGCCTCAATCCCCCGGTTATAGCTTTTAACCCCCGTTACATCTg                              >  1:4760722/1‑140 (MQ=255)
                                                                                                           ggCAGAAACACTGCTCACGCAACTGCAGTAGACGTTTCATGTTGCCACGTTGTTAATATCGGACACCACCTGTCCGCATCGCTATGTGCTCGTGTGCCTCAATCCCCCGGTTATAGCTTTTCACCCCCGTTACCTCTggg                            >  2:8130719/1‑139 (MQ=255)
                                                                                                           ggCAGAAACACTGCTCACGCAACTGCAGTAGACGTTTCATGTTGCCACGTTGTTAATATCGGACACCACCTGTCCGCATCGCTATGTGCTCGTGTGCCTCAATCCCCCGGTTATAGCTTTTAAcc                                           <  1:4690389/125‑1 (MQ=255)
                                                                                                           ggCAGAAACACTGCTCACGCAACTGCAGTAGACGTTTCATGTTGCCACGTTGTTAATATCGGACACCACCTGTCCGCATCGCTATGTGCTCGTGTGCCTCAATCCCCCGGTTATAGCTTTTAACCCCCGTGACATCTGGc                            >  1:7979581/1‑140 (MQ=255)
                                                                                                           ggCAGAAACACTGCTCACGCAACTGCAGTAGACGTTTCATGTTGCCACGTTGTTAATATCGGACACCACCTGTCCGCATCGCTATGTGCTCGTGTGCCTCAATCCCCCGGTTATAGCTGTTAACCCCCGGTTCATCTGGc                            >  1:3126954/1‑140 (MQ=255)
                                                                                                                aaaCACTGCTCACGCAACTGCAGTAGACGTTTCATGTTGCCACGTTGTTAATATCGGACACCACCTGTCCGCATCGCTATGTGCTCGTGTGCCTCAATCCCCCGGTTATAGCTTTTAACCCCCGTTACATCTGGCTTTTg                       <  2:2161132/140‑1 (MQ=255)
                                                                                                                   cacTGCTCACGCAACTGCAGTAGACGTTTCATGTTGCCACGTTGTTAATATCGGACACCACCTGTCCGCATCGCTATGTGCTCGTGTGCCTCAATCCCCCGGTTATAGCTTTTAACCCCCGTTACATCTGGCTTTTGCAg                    <  1:6506030/140‑1 (MQ=255)
                                                                                                                     cTGCTCACGCAACTGCAGTAGACGTTTCATGTTGCCACGTTGTTAATATCGGACACCACCTGTCCGCATCGCTATGTGCTCGTGTGCCTCAATCCCCCGGTTATAGCTTTTAACCCCCGTTACATCTGGCTTTTGCAGaa                  <  2:1616926/140‑1 (MQ=255)
                                                                                                                      tGCTCACGCAACTGCATTAGACGTTTCATGTTGCCACGTTGTTAATATCGGACACCACCTGTCCGCATCGCTATGTGCTCGTGTGCCTCAATCCCCCGGGTATAGCTTTTAACAACCGGGGGATCTGGCTGTTGCAgtgt                 >  2:5796885/1‑137 (MQ=25)
                                                                                                                      tGCTCACGCAACTGCAGTAGACGTTTCATGTTGCCACGTTGTTAATATCGGACACCACCTGTCCGCCTCGCTATGTGCTCGTGTGCCTCAATCCCCCGGTTATAGCTTTTAACCGCCGGTACTTCTGGGTTTTGCAGaaa                 >  1:2849250/1‑140 (MQ=38)
                                                                                                                      tGCTCACGCAACTGCAGTAGACGTTTCATGTTGCCACGTTGTTAATATCGGACACCACCTGTCCGCATCGCTATGTGCTCGTGTGCCTCAATCCCCCGGTTATAGGTTTTATGCGGCGTTTGATCTGGCGTTTGCAGaaa                 >  2:1299633/1‑140 (MQ=25)
                                                                                                                      tGCTCACGCAACTGCAGTAGACGTTTCATGTTGCCACGTTGTTAATATCGGACACCACCTGACCGCATCGCTATGTGCTCGTGTGCCTCAATCCCCCGGTTAGAGCTTTTAACCCCCGTTACATCTGGCTTTTGCAGaac                 >  2:7105390/1‑139 (MQ=255)
                                                                                                                       gCTCACGCAACTGCAGTAGACGTTTCATGTTGCCACGTTGTTAATATCGGACACCACCTGTCCGCATCGCTATGTGCTCGTGTGCCTCAATCCCCCGGTTATAGCTTTTAACCCCCGTTACATCTGGCTTTTGCAGAAAt                >  2:9073770/1‑140 (MQ=255)
                                                                                                                        cTCACGCAACTGCAGTAGACGTTTCATGTTGCCACGTTGTTAATATCGGACACCACCTGTCCGCATCGCTCTGTGCTCGTGTGCCTCAATCCCCCGGTTATAGCTTGTCACCCCCGGTACATCTGGCTGTTGCAGAAAtt               >  2:9254550/1‑139 (MQ=37)
                                                                                                                           aCGCAACTGCAGTAGACGTTTCATGTTGCCACGTTGTTAATATCGGACACCACCTGTCCGCATCGCTATGTGCTCGTGTGCCTCAATCCCCCGGTTATAGCTTTTAACCCCCGTTACATCTGGCTTTTGCAGAAATaaaa            <  1:8388631/140‑1 (MQ=255)
                                                                                                                             gCAACTGCAGTAGACGTTTCATGTTGCCACGTTGTTAATATCGGACACCACCTGTCCGCATCGCTATGTGCTCGTGTGCCTCAATCCCCCGGTTATAGCTTTTAACCCCCGTTACATCTGGCTTTTGCAGAAATaaaaaa          <  1:9432294/140‑1 (MQ=255)
                                                                                                                              cAACTGCAGTAGACGTTTCATGTTGCCACGTTGTTAATATCGGACACCACCTGTCCGCATCGCTATGTGCTCGTGTGCCTCAATCCCCCGGTTATAGCTTTTAACCCCCGTTACATCTGGCTTTTGCAGAAATAAAAAAt         <  1:1069944/140‑1 (MQ=255)
                                                                                                                                aCTGCAGTAGACGTTTCATGTTGCCACGTTGTTAATATCGGACACCACCTGTCCGCATCGCTATGTGCTCGTGTGCCTCAATCCCCCGGTTATAGCTTTTAACCCCCGTTACTTCTGGGGTTTGCAGATATAAAGAGTAg       >  1:1141070/1‑140 (MQ=38)
                                                                                                                                aCTGCAGTAGACGTTTCATGTTGCCACGTTGTTAATATCGGACACCACCTGTCCGCATCGCTATGTGCTCGTGTGCCTCAATCCCCCGGTTATAGCTTTTAACCCCCGTTACATCTGGCTTTTGCAGAAATAATATATAg       >  1:7216969/1‑140 (MQ=255)
                                                                                                                                   gCAGTAGACGTTTCCTGTTGCCACGTTGTTAAGATCGGACACCACCTGTCCGCATCGCTATGTGCTCGTGTGCCTCAATCCCCCGGTTATAGCTTTTAACCCCCGTTACATCTGGCTTTTGCAGAAATAAAAAATAGttt    <  2:464491/140‑1 (MQ=255)
                                                                                                                                   gCAGTAGACGTTTCATGTTGCCACGTTGTTAATATCGGACACCACCTGTCCGCATCGCTATGTGCTCGTGTGCCTCAATCCCCCGGTTATAGCTTTTAACCCCCGTTACATCTGGCTTTTGCAGAAATAAAAAATAGttt    <  2:3929407/140‑1 (MQ=255)
                                                                                                                                   gCAGTAGACGTTTCATGTTGCCACGTTGTTAATATCGGACACCACCTGTCCGCATCGCTATGTGCTCGTGTGCCTCAATCCCCCGGTTATAGCTTTTAACCCCCGTTACATCTGGCTTTTGCAGAAATAAAAAATAGttt    <  1:1014240/140‑1 (MQ=255)
                                                                                                                                         |                                                                                                                                       
ATTAAGGGCGGACTCAGTGGGCGTAATGCTAAAGGCGGACGAACTCATACTCGTGCCGTGCGTGGCATTGACGGGGATGTGAAGCTTAACCGTGCGCTGTGGGTGATGGCAGAAACACTGCTCACGCAACTGCAGTAAACGTTTCATGTTGCCACGTTGTTAATATCGGACACCACCTGTCCGCATCGCTATGTGCTCGTGTGCCTCAATCCCCCGGTTATAGCTTTTAACCCCCGTTACATCTGGCTTTTGCAGAAATAAAAAATAGTTTCT  >  NZ_CP025268/2773373‑2773645

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑