BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP025268	2,940,107	G→A	G112D (GGC→GAC)	fucK →	L‑fuculokinase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP025268	2,940,107	0	G	A	99.7%	2603.2 / NA	665	G112D (GGC→GAC)	fucK	L‑fuculokinase
Reads supporting (aligned to +/- strand): ref base G (0/0); major base A (260/402); minor base T (1/1); total (261/404)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 665 total aligned reads displayed.

GTGAACTGACTGAATGCCACATCCGCGGTATCGCCGTCACCACCTTTGGTGTGGATGGCGCTCTGGTAGATAAGCAAGGCAATCTGCTCTATCCGATTATTAGCTGGAAATGTCCGCGAACAGCAGCGGTTATGGGCAATATTGAACGGTTAATCTCCGCACAGCGGTTGCAGGCTATTTCTGGCGTCGGAGCCTTTAGTTTCAATACGTTATATAAGTTGGTGTGGTTGAAAGAAAATCATCCACAACTGCTGGAACGCGCGCACGCCTGG  >  NZ_CP025268/2939972‑2940243

 tGAACTGACTGAATGCCACATCCGCGGTATCGCCGTCACCACCTTTGGTGTGGATGGCGCTCTGGTAGATAAGCAAGGCAATCTGCTCTATCCGATTATTAGCTGGAAATGTCCGCGAACAGCAGCGGTTATGGACAata                                                                                                                                     <  1:289119/140‑1 (MQ=255)

   aaCTGACTGAATGCCACATCCGCGGTATCGCCGTCACCACCTTTGGTGTGGATGGCGCTCTGGTAGATAAGCAAGGCAATCTGCTCTATCCGATTATTAGCTGGAAATGTCCGCGAACAGCAGCGGTTCTGGACAATAtt                                                                                                                                   >  2:9226283/1‑140 (MQ=255)

   aaCTGACTGAATGCCACATCCGCGGTATCGCCGTCACCACCTTTGGTGTGGATGGCGCTCTGGTAGATAAGCAAGGCAATCTGCTCTATCCGATTATTAGCTGGAAATGTCCGCGAACAGCAGCGGTTATGGACAATAtt                                                                                                                                   >  1:1252309/1‑140 (MQ=255)

   aaCTGACTGAATGCCACATCCGCGGTATCGCCGTCACCACCTTTGGTGTGGATGGCGCTCTGGTAGATAAGCAAGGCAATCTGCTCTATCCGATTATTAGCTGGAAATGTCCGCGAACAGCAGCGGTTATGGACAATAtt                                                                                                                                   >  2:2735781/1‑140 (MQ=255)

   aaCTGACTGAATGCCACATCCGCGGTATCGCCGTCACCACCTGTGGTGTGGATGGCGCTCTGGTAGATAAGCAAGGCAATCTGCTCTATCCGATTATTAGCTGGAAATGTCCGCGAACAGCAGCGGTTATGGACAATAtt                                                                                                                                   >  1:1562428/1‑140 (MQ=255)

      tgactgaATGCCACATCCGCGGTATCGCCGTCACCACCTTTGGTGTGGATGGCGCTCTGGTAGATAAGCAAGGCAATCTGCTCTATCCGATTATTAGCTGGAAATGTCCGCGAACAGCAGCGGTTATGGACAATATTGaa                                                                                                                                <  2:5952312/140‑1 (MQ=255)

      tgactgaATGCCACATCCGCGGTATCGCCGTCACCACCTTTGGTGTGGATGGCGCTCTGGTAGATAAGCAAGGCAATCTGCTCTATCCGATTATTAGCTGGAAATGTCCGCGAACAGCAGCGGTTATGGACAATATTGaa                                                                                                                                <  2:9273184/140‑1 (MQ=255)

      tgactgaATGCCACATCCGCGGTATCGCCGTCACCACCTTTGGTGTGGATGGCGCTCTGGTAGATAAGCAAGGCAATCTGCTCTATCCGATTATTAGCTGGAAATGTCCGCGAACAGCAGCGGTTATGGACAATATTGaa                                                                                                                                <  1:7640626/140‑1 (MQ=255)

      tgactgaATGCCACATCCGCGGTATCGCCGTCACCACCTTTGGTGTGGATGGCGCTCTGGTAGATAAGCAAGGCAATCTGCTCTATCCGATTATTAGCTGGAAATGTCCGCGAACAGCAGCGGTTATGGACAATATTGaa                                                                                                                                >  2:3213909/1‑140 (MQ=255)

      tgactgaATGCCACATCCGCGGTATCGCCGTCACCACCTTTGGTGTGGATGGCGCTCTGGTAGATAAGCAAGGCAATCTGCTCTATCCGATTATTAGCTGGAAATGTCCGCGAACAGCAGCGGTTATGGACAATATTGaa                                                                                                                                <  2:4298265/140‑1 (MQ=255)

      cgacggaCTGCCACATCCGCGGGATCGCCGTCACCACCTTTGGTGTGGATGGCGCTCTGGTAGATAAGCAAGGCAATCTGCTCTATCCGATTATTAGCTGGAAATGTCCGCGAACAGCAGCGGTTATGGACAATATTGaa                                                                                                                                <  1:9385002/139‑1 (MQ=255)

        actgaATGCCACATCCGCGGTATCGCCGTCACCACCTTTGGTGTGGATGGCGCTCTGGTAGATAAGCAAGGCAATCTGCTCTATCCGATTATTAGCTGGAAATGTCCGCGAACAGCAGCGGTGATGGACAATATTGAACg                                                                                                                              >  2:5040470/1‑140 (MQ=255)

        actgaATGCAACATCAGAGGTATCGCCGTCACCACCTTTGGTGAGGATTGCGCTCTGTTAGATAAGAAAGGCAATCTGCTCTATCCGATTATTAGCTGGACATGTCCGCGAACAGCAGCGCATATGGACAATATTGAACg                                                                                                                              >  2:4498656/1‑140 (MQ=255)

         ctgaATGCCACATCCGCGGTATCGCCGTCACCACCTTTGGTGTGGATGGCGCTCTGGTAGATAAGCAAGGCAATCTGCTCTATCCGATTATTAGCTGGAAATGTCCGCGAACAGCAGCGGTTATGGACAATATTGAACgg                                                                                                                             <  2:304669/140‑1 (MQ=255)

           gaATGCCACATCCGCGGTATCGCCGTCACCACCTTTGGTGTGGATGGCGCTCTGGTAGATAAGCAAGGCAATCTGCTCTATCCGATTATTAGCTGGAAATGTCCGCGAACAGCAGCGGTTCTGGACAATATTGACCGGtt                                                                                                                           >  1:1075087/1‑140 (MQ=255)

           gaATGCCACATCCGCGGTATCGCCGTCACCACCTTTGGTGTGGATGGCGCTCTGGTAGATAAGCAAGGCAATCTGCTCTATCCGATTATTAGCTGGAAATGTCCGCGAACAGCAGCGGTTATGGACAATATTGAACGGtt                                                                                                                           <  2:8351918/140‑1 (MQ=255)

           gaATGCCACATCCGCGGTATCGCCGTCACCACCTTTGGTGTGGATGGCGCTCTGGTAGATAAGCAAGGCAATCTGCTCTATCCGATTATTAGCTGGAAATGTCCGCGAACAGCAGCGGTTATGGACAATATTGAACGGtt                                                                                                                           >  2:3944289/1‑140 (MQ=255)

           gaATGCCACATCCGCGGTATCGCCGTCACCACCTTTGGTGTGGATGGCGCTCTGGTAGATAAGCAAGGCAATCTGCTCTATCCGATTATTAGCTGGAAATGTCCGCGAACAGCAGCGGTTATGGACAATATTGAACGGtt                                                                                                                           <  2:6504730/140‑1 (MQ=255)

                ccACATCCGCGGTATCGCCGTCACCACCTTTGGTGTGGATGGCGCTCTGGTAGATAAGCAAGGCAATCTGCTCTATCCGATTATTAGCTGGAAATGTCCGCGAACAGCAGCGGTTATGGACAATATTGAACGGTTAAtct                                                                                                                      <  2:1254406/140‑1 (MQ=255)

                  acaTCCGCGGTATCGCCGTCACCACCTTTGGTGTGGATGGCGCTCTGGTAGATAAGCAAGGCAATCTGCTCTATCCGATTATTAGCTGGAAATGTCCGCGAACAGCAGCGGTTATGGACAATATTGAACGGTTAATCTcc                                                                                                                    >  2:4737777/1‑140 (MQ=255)

                  acaTCCGCGGTATCGCCGTCACCACCTTTGGTGTGGATGGCGCTCTGGTAGATAAGCAAGGCAATCTGCTCTATCCGATTATTAGCTGGAAATGTCCGCGAACAGCAGCGGTTATGGACAATATTGAACGGTTAATCTcc                                                                                                                    >  1:4391173/1‑140 (MQ=255)

                  acaTCCGCGGTATCGCCGTCACCACCTTTGGTGTGGATGGCGCTCTGGTAGATAAGCAAGGCAATCTGCTCTATCCGATTATTAGCTGGAAATGTCCGCGAACAGCAGCGGTTATGGACAATATTGAACGGTTAATCTcc                                                                                                                    >  1:9236271/1‑140 (MQ=255)

                      ccGCGGTATCGCCGTCACCACCTTTGGTGTGGATGGCGCTCTGGTAGATAAGCAAGGCAATCTGCTCTATCCGATTATTAGCTGGAAATGTCCGCGAACAGCAGCGGTTATGGACAATATTGAACGGTTAATCTCCGcac                                                                                                                >  2:5001486/1‑140 (MQ=255)

                        gcgGTATCGCCGTCACCACCTTTGGTGTGGATGGCGCTCTGGTAGATAAGCAAGGCAATCTGCTCTATCCGATTATTAGCTGGAAATGTCCGCGAACAGCAGCGGTTATGGACAATATTGAACGGTTAATCTCCGCACAg                                                                                                              <  1:4619028/140‑1 (MQ=255)

                        gcgGTATCGCCGTCACCACCTTTGGTGTGGATGGCGCTCTGGTAGATAAGCAAGGCAATCTGCTCTATCCGATTATTAGCTGGAAATGTCCGCGAACAGCAGCGGTTATGGACAATATTGAACGGTTAATCTCCGCACAg                                                                                                              >  2:5874290/1‑140 (MQ=255)

                         cgGTATCGCCGTCACCACCTTTGGTGTGGATGGCGCTCTGGTAGATAAGCAAGGCAATCTGCTCTATCCGATTATTAGCTGGAAATGTCCGCGAACAGCAGCGGTTATGGACAATATTGAACGGTTAATCTCCGCACAGc                                                                                                             >  1:66911/1‑140 (MQ=255)

                         cgGTATCGCCGTCACCACCTTTGGTGTGGATGGCGCTCTGGTAGATAAGCAAGGCAATCTGCTCTATCCGATTATTAGCTGGAAATGTCCGCGAACAGCAGCGGTTATGGACAATATTGAACGGTTAATCTCCGCACAGc                                                                                                             >  1:8915344/1‑140 (MQ=255)

                           gTATCGCCGTCACCACCTTTGGTGTGGATGGCGCTCTGGTAGATAAGCAAGGCAATCTGCTCTATCCGATTATTAGCTGGAAATGTCCGCGAACAGCAGCGGTTATGGACAATATTGAACGGTTAATCTCCGCACAGCgg                                                                                                           >  1:9132288/1‑140 (MQ=255)

                              tCGCCGTCACCACCTTTGGTGTGGATGGCGCTCTGGTAGATAAGCAAGGCAATCTGCTCTATCCGATTATTAGCTGGAAATGTCCGCGAACAGCAGCGGTTATGGACAATTTTGAACGGTTAATCTCCGCACAGCGGTTg                                                                                                        >  2:6965426/1‑140 (MQ=255)

                              tCGCCGTCACCACCTTTGGTGTGGATGGCGCTCTGGTAGATAAGCAAGGCAATCTGCTCTATCCGATTATTAGCTGGAAATGTCCGCGAACAGCAGCGGTTATGGACAATATTGAACGGTTAATCTCCGCACAGCGGTTg                                                                                                        <  1:5190978/140‑1 (MQ=255)

                                 ccGTCACCACCTTTGGTGTGGATGGCGCACTGGTAGATAAGCAAGGCAATCTGCTCTATCCGATTATTAGCTGGAAATGTCCGCGAACAGCAGCGGTTATGGACAATATTGAACGGTTAATCTCCGCACAGCGGTTGCAg                                                                                                     <  1:1936389/140‑1 (MQ=255)

                                              tGGTGTGGATGGCGCTCTGGTAGATAAGCAAGGCAATCTGCTCTATCCGATTATTAGCTGGAAATGTCCGCGAACAGCAGCGGTTATGGACAATATTGAACGGTTAATCTCCGCACAGCGGTTGCAGGCTATTTCTGGCg                                                                                        >  1:204875/1‑140 (MQ=255)

                                                 tgtgGATGGCGCTCTGGTAGATAACCAAGGCAATCTGCTCTATCCGATTATGAGCTGGAAATGTCCGCGAACAGCAGCGGTTATGGACAATATTGAACGGTTAATCTCCGCACAGCGGTCGCAGGCTATTTCTGGCGTCg                                                                                     <  1:6436082/140‑1 (MQ=255)

                                                    ggATGGCGCTCTGGTAGATAAGCAAGGCAATCTGCTCTATCCGATTATTAGCTGGAAATGTCCGCGAACAGCAGCGGTTATGGACAATATTGAACGGTTAATCTCCGCACAGCGGTTGCAGGCTATTTCTGGCGTCGGAg                                                                                  <  1:3482545/140‑1 (MQ=255)

                                                     gATGGCGCTCTGGTAGATAAGCAAGGCAATCTGCTCTATCCGATTATTAGCTGGAAATGTCCGCGAACAGCAGCGGTTATGGACAATATTGAACGGTTAATCTCCGCACAGCGGTTGCAGGCTATTTCTGGCGTCGGAGc                                                                                 >  1:6016418/1‑140 (MQ=255)

                                                       tGGCGCTCTGGTAGATAAGCAAGGCAATCTGCTCTATCCGATTATTAGCTGGAAATGTCCGCGAACAGCAGCGGTTATGGACAATATTGAACGGTTAATCTCCGCACAGCGGTTGCAGGCTATTTCTGGCGTCGGAGCCt                                                                               <  1:3283157/140‑1 (MQ=255)

                                                        ggCGCTCTGGTAGATAAGCAAGGCAATCTGCTCTATCCGATTATTAGCTGGAAATGTCCGCGAACAGCAGCGGTTATGGACAATATTGAACGGTTAATCTCCGCACAGCGGTTGCAGGCTATTTCTGGCGTCGGAGCCtt                                                                              <  1:1817226/140‑1 (MQ=255)

                                                         gcgcTCTGGTAGATAAGCAAGGCAATCTGCTCTATCCGATTATTAGCTGGAAATGTCCGCGAACAGCAGCGGTTATGGACAATATTGAACGGTTAATCTCCGCACAGCGGTTGCAGGCTATTTCTGGCGTCGGAGCCttt                                                                             <  1:1393301/140‑1 (MQ=255)

                                                          cgcTCTGGTAGATAAGCAAGGCAATCTGCTCTATCCGATTATTAGCTGGAAATGTCCGCGAACAGCAGCGGTTATGGACAATATTGaa                                                                                                                                <  1:5715485/88‑1 (MQ=255)

                                                               tGGTAGATAAGCAAGGCAATCTGCTCTATCCGATTATTAGCTGGAAATGTCCGCGAACAGCAGCGGTTATGGACAATATTGAACGGGTAATCTCCGCACAGCGGTTGCAGGCTATTTCTGGCGTCGGAGCCTTTAGTTTc                                                                       >  2:8144135/1‑140 (MQ=255)

                                                                ggTAGATAAGCAAGGCAATCTGCTCTATCCGATTATTAGCTGGAAATGTCCGCGAACAGCAGCGGTTATGGACAATATTGAACGGTTAATCTCCGCACAGCGGTTGCAGGCTATTTCTGGCGTCGGAGCCTTTAGTTTCa                                                                      <  2:7069481/140‑1 (MQ=255)

                                                                ggTAGATAAGCAAGGCAATCTGCTCTATCCGATTATTAGCTGGAAATGTCCGCGAACAGCAGCGGTTATGGACAATATTGAACGGTTAATCTCCGCACAGCGGTTGCAGGCTATTTCTGGCGTCGGAGCCTTTAGTTTCa                                                                      <  1:1009413/140‑1 (MQ=255)

                                                                ggTAGATAAGCAAGGCAATCTGCTCTATCCGATTATTAGCTGGAAATGTCCGCGAACAGCAGCGGTTATGGACAATATTGAACGGTTAATCTCCGCACAGCGGTTGCAGGCTATTTCTGGCGTCGGAGCCTTTAGTTTCa                                                                      <  1:1606763/140‑1 (MQ=255)

                                                                ggTAGATAAGCAAGGCAATCTGCTCTATCCGATTATTAGCTGGAAATGTCCGCGAACAGCAGCGGTTATGGACAATATTGAACGGTTAATCTCCGCACAGCGGTTGCAGGCTATTTCTGGCGTCGGAGCCTTTAGTTTCa                                                                      <  2:7224124/140‑1 (MQ=255)

                                                                ggTAGATAAGCAAGGCAATCTGCTCTATCCGATTATTAGCTGGAAATGTCCGCGAACAGCAGCGGTTATGGACAATATTGAACGGTTAATCTCCGCACAGCGGTTGCAGGCTATTTCTGGCGTCGGAGCCTTTAGTTTCa                                                                      <  1:4825861/140‑1 (MQ=255)

                                                                   aGATAATCAAGGCAATCTGCTCTATCCGAGTATTAGCTGGAAATGTCCGCGAACAGCAGCGGTTATGTACTATATTGAACGGTGAATATCCGCACATCGGTTGCAGGCTATTTCTTGCGTCGGAGCCTTTAGTTTCAATa                                                                   <  2:8637702/140‑1 (MQ=255)

                                                                   aGATAAGCAAGGCAATCTGCTCTATCCGATTATTAGCTGGAAATGTCCGCGAACAGCAGCGGTTATGGACAATATTGAACGGTTAATCTCCGCACAGCGGTTGCAGGCTATTTCTGGCGTCGGAGCCTTTAGTTTCAATa                                                                   <  1:7557243/140‑1 (MQ=255)

                                                                   aGATAAGCAAGGCAATCTGCTCTATCCGATTATTAGCTGGAAATGTCCGCGAACAGCAGCGGTTATGGACAATATTGAACGGTTAATCTCCGCACAGCGGTTGCAGGCTATTTCTGGCGTCGGAGCCTTTAGTTTCAATa                                                                   <  1:633887/140‑1 (MQ=255)

                                                                   aGATAAGCAAGGCAATCTGCTCTATCAGATTATTAGCTGTAAATGTCCGCGAACAGCAGCGTTTATGGACAATATTGAACTGTTAATCTCCGCACAGCGGTTGCAGGCTATTTCTGGCGTCGGAGCCTTTAGTTTCAATa                                                                   <  2:9378395/140‑1 (MQ=255)

                                                                     aTAATCAAGGCAATCTGCTCTATCCGATTATTAGCTGGAAATGTCCGCGTACAGCAGCGGTTATGGACAATATTGAACGGTTAATCTCCGCACAGCGGTTGAAGGCTATTTCTGGCGTCGGATCCTTTAGTTTCAATAct                                                                 <  2:877384/140‑2 (MQ=255)

                                                                     aTAAGCAAGGCAATCTTCTCTATCCGATTATTAGCTGGAAATGTCCGCGAACAGCAGCGGTTATGGACAATATTGAACGGTTAATCTCCGCACAGCGGTTGCAGGCTATTTCTGGCGTCGGAGCCTTTAGTTTCAATACg                                                                 <  2:6755364/140‑1 (MQ=255)

                                                                     aTAAGCAAGGCAATCTGCTCTATCCGATTATTAGCTGGAAATGTCCTCGAACAGCAGCGGTTATTGACAATATTGAACGGTTAATCTCCGCACAGCGGTTGCAGGATATTTCTGGCGTCGGAGCCTTTAGTTTCAATACg                                                                 <  2:7933296/140‑1 (MQ=255)

                                                                     aTAAGCAAGGCAATCTGCTCTATCCGATTATTAGCTGGAAATGTCCGCGAACAGCAGCGGTTATGGACAATATTGAACGGTTAATCTCCGCACAGCGGTTGCAGGCTATTTCTGGCGTCGGAGCCTTTAGTTTCAATACg                                                                 <  1:2274118/140‑1 (MQ=255)

                                                                     aTAAGCAAGGCAATCTGCTCTATCCGATTATTAGCTGGAAATGTCCGCGAACAGCAGCGGTTATGGACAATATTGAACGGTTAATCTCCGCACAGCGGTTGCAGGCTATTTCTGGCGTCGGAGCCTTTAGTTTCAATACg                                                                 <  2:893817/140‑1 (MQ=255)

                                                                     aTAAGCAAGGCAATCTGCTCTATCCGATTATTAGCTGGAAATGTCCGCGAACAGCAGCGGTTATGGACAATATTGAACGGTTAATCTCCGCACAGCGGTTGCAGGCTATTTCTGGCGTCGGAGCCTTTAGTTTCAATACg                                                                 <  1:486912/140‑1 (MQ=255)

                                                                     aTAAGCAAGGCAATCTGCTCTATCCGATTATTAGCTGGAAATGTCCGCGAACAGCAGCGGTTATGGACAATATTGAACGGTTAATCTCCGCACAGCGGTTGCAGGCTATTTCTGGCGTCGGAGCCTTTAGTTTCAATACg                                                                 <  2:1592574/140‑1 (MQ=255)

                                                                     aTAAGCAAGGCAATCTGCTCTATCCGATTATTAGCTGGAAATGTCCGCGAACAGCAGCGGTTATGGACAATATTGAACGGTTAATCTCCGCACAGCGGTTGCAGGCTATTTCTGGCGTCGGAGCCTTTAGTTTCAATACg                                                                 <  2:5577725/140‑1 (MQ=255)

                                                                     aTAAGCAAGGCAATCTGCTCTATCCGATTATTAGCTGGAAATGTCCGCGAACAGCAGCGGTTATGGACAATATTGAACGGTTAATCTCCGCACAGCGGTTGCAGGCTATTTCTGGCGTCGGAGCCTTTAGTTTCAATACg                                                                 <  2:2456536/140‑1 (MQ=255)

                                                                     aTAAGCAAGGCAATCTGCTCTATCCGATTATTAGCTGGAAATGTCCGCGAACAGCAGCGGTTATGGACAATATTGAACGGTTAATCTCCGCACAGCGGTTGCAGGCTATTTCTGGCGTCGGAGCCTTTAGTTTCAATACg                                                                 <  1:7062539/140‑1 (MQ=255)

                                                                     aTAAGCAAGGCAATCTGCTCTATCCGATTATTAGCTGGAAATGTCCGCGAACAGCAGCGGTTATGGACAATATTGAACGGTTAATCTCCGCACAGCGGTTGCAGGCTATTTCTGGCGTCGGAGCCTTTAGTTTCAATACg                                                                 <  2:3440207/140‑1 (MQ=255)

                                                                     aTAAGCAAGGCAATCTGCTCTATCCGATGATTATCTGGAAATGTCCGAGAACATCAGCGTTTTTGGACAATATTGAACGGTTAATCTCCGCACAGCGGTTGCAGGCTATTTCTGGCGTCGGAGCCTTTAGTTTCTATACg                                                                 <  2:7713793/140‑1 (MQ=255)

                                                                         gCAAGGCAATCTGCTCTATCCGATTATTAGCTGGAAATGTCCGCGAACAGCAGCGGTTATGGACAATATTGAACGGTTAATCTCCGCACAGCGGTTGCAGGCTATTTCTGGCGTCGGAGCCTTTAGTTTCAATACGTtat                                                             <  2:3660119/140‑1 (MQ=255)

                                                                         gCAAGGCAATCTGCTCTATCCGATTATTAGCTGGAAATGTCCGCGAACAGCAGCGGTTATGGACAATATTGAACGGTTAATCTCCGCACAGCGGTTGCAGGCTATTTCTGGCGTCGGAGCCTTTAGTTTCAATACGTtat                                                             <  2:7503299/140‑1 (MQ=255)

                                                                         gCAAGGCAATCTGCTCTATCCGATTATTAGCTGGAAATGTCCGCGAACAGCAGCGGTTATGGACAATATTGAACGGTTAATCTCCGCACAGCGGTTGCAGGCTATTTCTGGCGTCGGAGCCGTTAGTGTCAATACGTgat                                                             >  1:2397814/1‑140 (MQ=255)

                                                                            aGGCAATCTGCTCTATCCGATTATTAGCTGGAAATGTCCGCGAACAGCAGCGGTTATGGACAATATTGAACGGTTAATCTCCGCACAGCGGTTGCAGGCTATTTCTGGCGTCGGAGCCTTTAGTTTCAATACGTtatata                                                          >  1:3411736/1‑140 (MQ=255)

                                                                            aGGCAATCTGCTCTATCCGATTATTAGCTGGAAATGTCCGCGAACAGCAGCGGTTATGGACAATATTGAACGGTTAATCTCCGCACAGCGGTTGCAGGCTATTTCTGGCGTCGGAGCCTTTAGTTTCAATACGTtatata                                                          <  2:2025543/140‑1 (MQ=255)

                                                                            aGGCAATCTGCTCTATCCGATTATTAGCTGGAAATGTCCGCGAACAGCAGCGGTTATGGACAATATTGAACGGTTAATCTCCGCACAGCGGTTGCAGGCTATTTCTGGCGTCGGAGCCTTTAGTTTCAATACGTtatata                                                          <  1:8626603/140‑1 (MQ=255)

                                                                                aaTCTGCTCGATCCGTGTATTCGCTGGAAATGTCCGCGAACAGCAGCGGTTATGGACAATATTGACCGGGTAATCTCCGCACAGCGGTTGCAGGCTCTTTCTGGGGTCGGAGCGTTTAGGGTCAATACGGTAGATAAGtt                                                      >  2:802594/1‑140 (MQ=255)

                                                                                 aTCTGCTCTATCCGATTATTAGCTGGAAATGTCCGCGAACAGCAGCGGTTATGGACAATATTGAACGGTTAATCTCCGCACAGCGGTTGCTGGCTATTTCTGGCGTCGGAGCCTTTAGTTTCAATACGTTATATAAGTTg                                                     <  2:1901878/140‑1 (MQ=255)

                                                                                   cggCTCTATCCGATTATGAGCTGGAAATGTCCGCGAACAGCAGCGGTTATGGACAATATTGAACGGTTAATCTCCGCACAGCGGTTGCAGGCTATTTCTGGCGTCGGAGCCTTTAGTTTCAATACGTTATATAAGTTGgt                                                   <  1:4982693/138‑1 (MQ=255)

                                                                                   cTGCTCTATCCGATTATTAGCTGGAAATGTCCGCGAACAGCAGCGGTTATGGACAATATTGAACGGTTAATCTCCGCACAGCGGTTGCAGGCTATTTCTGGCGTCGGAGCCTTTAGTTTCAATACGTTATATAAGTTGgt                                                   <  2:6658537/140‑1 (MQ=255)

                                                                                    tGCTCTATCCGATTATTAGCTGGAAATGTCCGCGAACAGCAGCGGTTATGGACAATATTGAACGGTTAATCTCCGCACAGCGGTTGCAGGCTATTTCTGGCGTCGGAGCCTTTAGTTTCAATACGTTATATACGTTGgtg                                                  <  2:2276168/140‑1 (MQ=255)

                                                                                     gCTCTATCCGATTATTAGCTGGAAATGTCCGCGAACAGCAGCGGTTATGGACAATATTGAACGGTTAATCTCCGCACAGCGGTTGCAGGCTATTTCTGGCGTCGGAGCCTTTAGTTTCAATACGTTATATAAGTTGgtgt                                                 <  1:4140864/140‑1 (MQ=255)

                                                                                        ctATCCGATTATTAGCTGGAAATGTCCGCGAACAGCAGCGGTTATGGACAATATTGAACGGTTAATCTCCGCACAGCGGTTGCAGGCTATTTCTGGCGTCGGAGCCTTTAGTTTCAATACGTTATATAAGTTGGTGTGGt                                              <  1:3866845/140‑1 (MQ=255)

                                                                                            ccGATTATTAGCTGGAAATGTCCGAGAACAGCAGCGGTTATGGACAATATTGAACGGTTACTCTCCGAACAGCGGTTGCAGGCTAGTTCTTGCGTAGGAGCATTTAGTTTCAATAAGTTATATAAGTTGGTGTGGTTgaa                                          <  2:2961218/140‑1 (MQ=255)

                                                                                              gATTATTAGCTGGAAATGTCCGCGAACAGCAGCGGTTATGGACAATATTGAACGGTTAATCTCCGCACAGCGGTTGCAGGCTATTTCTGGCGTCGGAGCCTTTAGTTTCAATACGTTATATAAGTTGGTGTGGTTgaaag                                        >  2:5142787/1‑140 (MQ=255)

                                                                                                ttattaGCTGGAAATGTCCGCGAACAGCAGCGGTTATGGACACTATTGAACGGTTAATCTCCGCACAGCGGTTGCAGGCTATTTCTGGCGTCGGAGCCTTTAGTTTCAATACGTTATATAAGTTGGTGTGGTTgaaagaa                                      >  2:4066997/1‑140 (MQ=255)

                                                                                                ttattaGCTGGAAATGTCCGCGAACAGCAGCGGTTATGGACAATATTGAACGGTTAATCTCCGCACAGCGGTTGCAGGCTATTTCTGGCGTCGGAGCCTTTAGTTTCAATACGTTATATAAGTTGGTGTGGTTgaaagaa                                      >  1:3798898/1‑140 (MQ=255)

                                                                                                ttattaGCTGGAAATGTCCGCGAACAGCAGCGGTTATGGACAATATTGAACGGTTAATCTCCGCACAGCGGTTGCAGGCTATTTCTGGCGTCGGAGCCTTTAGTTTCAATACGTTATATAAGTTGGTGTGGTTgaaagaa                                      >  2:8479520/1‑140 (MQ=255)

                                                                                                ttattaGCTGGAAATGTCCGCGAACAGCAGCGGTTATGGACAATATTGAACGGTTAATCTCCGCACAGCGGTTGCAGGCTATTTCTGGCGTCGGAGCCTTTAGTTTCAATACGTTATATAAGTTGGTGTGGTTgaaagaa                                      >  1:1471096/1‑140 (MQ=255)

                                                                                                ttattaGCTGGAAATGTCCGCGAACAGCAGCGGTTATGGACAATATTGAACGGTTAATCTCCGCACAGCGGTTGCAGGCTATTTCTGGCGTCGGAGCCTTTAGTTTCAATACGTTATATAAGTTGGTGTGGTTgaaagaa                                      >  2:6310986/1‑140 (MQ=255)

                                                                                                ttattaGCTGGAAATGTCCGCGAACAGCAGCGGTTATGGACAATATTGAACGGTTAATCTCCGCACAGCGGTTGCAGGCTATTTCTGGCGTCGGAGCCTTTAGTTTCAATACGTTATATAAGTTGGTGTGGTTgaaagaa                                      >  1:5867741/1‑140 (MQ=255)

                                                                                                ttattaGCTGGAAATGTCCGCGAACAGCAGCGGTTATGGACAATATTGAACGGTTAATCTCCGCACAGCGGTTGCAGGCTATTTCTGGCGTCGGAGCCTTTAGTTTCAATACGTTATATAAGTTGGTGTGGTTgaaagaa                                      >  1:6837400/1‑140 (MQ=255)

                                                                                                    taGCTGGAAATGTCCGCGAACAGCAGCGGTTATGGACAATATTGAACGGTTAATCTCCGCACAGCGGTTGCAGGCTATTTCTGGCGTCGGAGCCTTTAGTTTCAATACGTTATATAAGTTGGTGTGGTTGAAAGAAAatc                                  <  2:1434400/140‑1 (MQ=255)

                                                                                                     aGCTGGAAATGTCCGCGAACAGCAGCGGTTATGGACAATATTg                                                                                                                                  <  1:7389892/43‑1 (MQ=255)

                                                                                                     aGCTGGAAATGTCCGCGAACAGCAGCGGTTATGGACAATATTGAACGGTTAATCTCCGCACAGCGGTTGCAGGCTATTTCTGGCGTCGGAGCCTTTAGTTTCAATACGTTATATAAGTTGGTGTGGTTGAAAGAAAatca                                 >  2:6155266/1‑140 (MQ=255)

                                                                                                     aGCTGGAAATGTCCGCGAACAGCAGCGGTTATGGACAATATTGAACGGTTAATCTCCGCACAGCGGTTGCAGGCTATTTCTGGCGTCGGAGCCTTTAGTTTCAATACGTTATATAAGTTGGTGTGGTTGAAAGAAAatca                                 >  2:1041967/1‑140 (MQ=255)

                                                                                                          gAAATGTCCGCGAACAGCAGCGGTTATGGACAATATTGAACGGTTAATCTCCGCACAGCGGTTGCAGGCTATTTCTGGCGTCGGAGCCTTTAGTTTCAATACGTTATATAAGTTGGTGTGGTTGAAAGAAAATCATCcac                            <  1:6181561/140‑1 (MQ=255)

                                                                                                          gAAATGTCCGCGAACAGCAGCGGTTATGGACAATATTGAACGGTTAATCTCCGCACAGCGGTTGCAGGCTATTTCTGGCGTCGGAGCCTTTAGTTTCAATACGTTATATAAGTTGGTGTGGTTGAAAGAAAATCATCcac                            <  1:1671646/140‑1 (MQ=255)

                                                                                                          gAAAGGTCCGCGAACAGCAGCGGTTATGGCCAATATTGAACGGTTAATCTCCGCACAGCGGTTGCAGGCTATTTCTGGCGTCGGAGCCTTTAGTTTCAATACGTTATATAAGTTGGTGTGGTTGAAAGAAAATCATCcac                            <  1:2613685/140‑1 (MQ=255)

                                                                                                                tCCCCGAACAGCAGCGGTTATGGACAATATTGAACGGTTAATCTCCGCACAGCGGTTGCAGGCTATTTCTGGCGTCGGAGCCTTTAGTTTCAATACGTTATATAAGTTGGTGTGGTTGAAAGAAAATCATCCACAActgc                      >  2:1137267/1‑140 (MQ=255)

                                                                                                                        cagcagCGGTTATGGACAATATTGAACGGTTAATCTCCGCACAGCGGTTGCAGGCTATTTCTGGCGTCGGAGCCTTTAGTTTCAATACGTTATATAAGTTGGTGTGGTTGAAAGAAAATCATCCACAACTGCTGGAAcgc              >  2:3863190/1‑140 (MQ=255)

                                                                                                                          gcagcGGTTATGGACAATATTGAACGGTTAATCTCCGCACAGCGGTTGCAGGCTATTTCTGGCGTCGGAGCCTTTAGTTTCAATACGTTATATAAGTTGGTGTTGTTGAAAGAAAATCATCCACAACTGCTGGAAcgcgc            <  2:4843345/140‑1 (MQ=255)

                                                                                                                          gcagcGGTGATGGACAATATTGAACGGTTAATCTCCGCACAGCGGTTGCAGGCTATATCTGGCGTCGGAGCCTTTAGTTTCAAAACGTTATCTAAGTTGGTGTGGTTGAAAGAAAATCATCCACAACTGCTGGAAcgcgc            <  1:5300637/140‑1 (MQ=255)

                                                                                                                           cagcGGTTATGGACAATATTGAACGGTTAATCTCCGCACAGCGGTTGCAGGCTATTTCTGGCGTCGGAGCCTTTAGTTTCAATACGTTATATAAGTTGGTGTGGTTGAAAGAAAATCATCCACAACTGCTGGAAcgcgcg           <  1:8995903/140‑1 (MQ=255)

                                                                                                                            agcGGTTATGGACAATATTGAACGGTTAATCTCCGCACAGCGGTTGCAGGCTATTTCTGGCGTCGGAGCCTTTAGTTTCAATACGTTATATAAGTTGGTGTGGTTGAAAGAAAATCATCCACAACTGCTGGAACgcgcgc          <  1:8380876/140‑1 (MQ=255)

                                                                                                                                gTTATGGACAATATTGAACGGTTAATCTCCGCACAGCGGTTGCAGGCTATTTCTGGCGTCGGAGCCTTTAGTTTCAATACGTTATATAAGTTGGTGTGGTTGAAAGAAAATCATCCACAACTGCTGGAACGCGCGCACGc      >  1:3620339/1‑140 (MQ=255)

                                                                                                                                 ttATGGACAATATTGAACGGTTAATCTCCGCACAGCGGTTGCAGGCTATTTCTGGCGTCGGAGCCTTTAGTTTCAATACGTTATATAAGTTGGTGTGGTTGAAAGAAAATCATCCACAACTGCTGGAACGCGCGCACGcc     <  1:3133863/140‑1 (MQ=255)

                                                                                                                                 ttATGGACAATATTGAACGGTTAATCTCCGCACAGCGGTTGCAGGCTATTTCTGGCGTCGGAGCCTTTAGTTTCAATACGTTATATAAGTTGGTGTGGTTGAAAGAAAATCATCCACAACTGCTGGAACGCGCGCACGcc     <  1:817273/140‑1 (MQ=255)

                                                                                                                                    tGGACAATATTGAACGGTTAATCTCCGCACAGCGGTTGCAGGCTATTTCTGGCGTCGGAGCCTTTAGTTTCAATACGTTATATAAGTTGGTGTGGTTGAAAGAAAATCATCCACAACTGCTGGAACGCGCGCACGCCTgg  <  2:4376869/140‑1 (MQ=255)

GTGAACTGACTGAATGCCACATCCGCGGTATCGCCGTCACCACCTTTGGTGTGGATGGCGCTCTGGTAGATAAGCAAGGCAATCTGCTCTATCCGATTATTAGCTGGAAATGTCCGCGAACAGCAGCGGTTATGGGCAATATTGAACGGTTAATCTCCGCACAGCGGTTGCAGGCTATTTCTGGCGTCGGAGCCTTTAGTTTCAATACGTTATATAAGTTGGTGTGGTTGAAAGAAAATCATCCACAACTGCTGGAACGCGCGCACGCCTGG  >  NZ_CP025268/2939972‑2940243

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`