BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP025268	3,255,705	A→G	234 (TAA→TAG)	yhaK →	pirin family protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP025268	3,255,705	0	A	G	98.9%	1637.5 / NA	472	234 (TAA→TAG)	yhaK	pirin family protein
Reads supporting (aligned to +/- strand): ref base A (0/0); major base G (270/197); minor base T (1/4); total (271/201)
Fisher's exact test for biased strand distribution p-value = 1.69e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 474 total aligned reads displayed.

AAATTTCATGCGCTTACGCATCATGAAGAGAAAGCGGCGCTGACCTGCGGTGATGGGGCGTTTATTCGTGACGAGGCTAACATTACGCTGGTTGCCGATTCCCCACTGCGCGCTTTGCTGATAGATTTGCCTGTCTAATTGTTTTTACAGGGAGATGATGATGAGTAAAAAATTGGCCAAAAAGCGCCAGCCGGTGAAGCCCGTGGTGGCGAAAGAACCTGCTCGCACCGCCAAAAATTTTGGCTATGAAGAGATGTTGAGCGAGCTGGAAGCT  >  NZ_CP025268/3255568‑3255841

 aagttCATGCGCTTACGCATTATGTAGAGATAGCGGCGCTGTCCTGCTGTGATGGGTCGTTTATTAGTGACGAGGCTAAAATTTCGCTGGTTGCCGATTCCCCACTGCGCGCTTTGCTGATAGATTTGCCTTTCTAGTTg                                                                                                                                       <  2:769439/137‑1 (MQ=255)

 aaTTTCATGCGCTTACGCATCATGAAGAGAAAGCGGCGCTGACCTGCGGTGATGGGGCGTTTATTCGTGACGAGGCTAACATTACGCTGGTTGCCGATTCCCCACTGCGCGCTTTGCTGATAGATTTGCCTGTCTAGTTg                                                                                                                                       <  1:1314202/140‑1 (MQ=255)

 aaTTTCATGCGCTTACGCATCATGAAGAGAAAGCGGCGCTGACCTGCGGTGATGGGGCGTTTATTCGTGACGAGGCTAACATTACGCTGGTTGCCGATTCCCCACTGCGCGCTTTGCTGATAGATTTGCCTGTCTAGTTg                                                                                                                                       <  1:6012408/140‑1 (MQ=255)

  aTTTCATGCGCTTACGCATCATGAAGAGAAAGCGGCGCTGACCTGCGGTGATGGGGCGTTTATTCGTGACGAGGCTAACATTACGCTGGTTGCCGATTCCCCACTGCGCGCTTTGCTGATAGATTTGCCTGTCTAGTTGt                                                                                                                                      >  2:4003652/1‑140 (MQ=255)

   tttCATGCGCTTACGCATCATGAAGAGAAAGCGGCGCTGACCTGCGGTGATGGGGCGTTTATTCGTGACGAGGCTAACATTACGCTGGTTGCCGATTCCCCACTGCGCGCTTTGCTGATAGATTTGCCTGTCTAgttgtt                                                                                                                                     <  2:5080152/140‑1 (MQ=255)

       aTGCGCTTACGCATCATGAAGAGAAAGCGGCGCTGACCTGCGGTGATGGGGCGTTTATTCGTGACGAGGCTAACATTACGCTGGTTGCCGATTCCCCACTGCGCGCTTTGCTGATAGATTTGCCTGTCTAGTTGTTTTTa                                                                                                                                 <  1:5693802/140‑1 (MQ=255)

         gcgcTTACGCATCATGAAGAGAAAGCGGCGCTGACCTGCGGTGATGGGGCGTTTATTCGTGACGAGGCTAACATTACGCTGGTTGCCGATTCCCCACTGCGCGCTTTGCTGATAGATTTGCCTGTCTAGTTGTTTTTACa                                                                                                                               <  2:6203389/140‑1 (MQ=255)

          cgcTTACGCATCATGAAGAGAAAGCGGCGCTGACCTGCGGTGATGGGGCGTTTATTCGTGACGAGGCTAACATTACGCTGGTTGCCGATTCCCCACTGCGCGCTTTGCTGATAGATTTGCCTGTCTAGTTGTTTTTACAg                                                                                                                              <  2:462198/140‑1 (MQ=255)

          cgcTTACGCATCATGAAGAGAAAGCGGCGCTGACCTGCGGTGATGGGGCGTTTATTCGTGACGAGGCTAACATTACGCTGGTTGCCGATTCCCCACTGCGCGCTTTGCTGATAGATTTGCCTGTCTAGTTGTTTTTACAg                                                                                                                              <  1:994172/140‑1 (MQ=255)

            cTTACGCATCATGAAGAGAAAGCGGCGCTGACCTGCGGTGATGGGGCGTTTATTCGTGACGAGGCTAACATTACGCTGGTTGCCGATTCCCCACTGCGCGCTTTGCTGATAGATTTGCCTGTCTAGTTGTTTTTACAggg                                                                                                                            <  1:506997/140‑1 (MQ=255)

              tACGCATCATGAAGAGAAAGCGGCGCTGACCTGCGGTGATGGGGCGTTTATTCGTGACGAGGCTAACATTACGCTGGTTGCCGATTCCCCACTGCGCGCTTTGCTGATAGATTTGCCTGTCTAGTTGTTTTTACAGGgag                                                                                                                          >  1:6811027/1‑140 (MQ=255)

              tACGCATCATGAAGAGAAAGCGGCGCTGACCTGCGGTGATGGGGCGTTTATTCGTGACGAGGCTAACATTACGCTGGTTGCCGATTCCCCACTGCGCGCTTTGCTGATAGATTTGCCTGTCTAGTTGTTTTTACAGGgag                                                                                                                          <  2:1227983/140‑1 (MQ=255)

                  catcatGAAGAGAAAGCGGCGCTGACCTGCGGTGATGGGGCGTTTATTCGTGACGAGGCTAACATTACGCTGGTTGCCGATTCCCCACTGCGCGCTTTGCTGATAGATTTGCCTGTCTAGTTGTTTTTGCAGGGAgatga                                                                                                                      <  1:448624/140‑1 (MQ=255)

                     catGAAGAGAAAGCGGCTCTGACCTGCGGTGATGGGGCGTTTATTCGTGACGAGGCTAACATTACGCTGGTTGCCGATTCCCCACTGCGCGCTTTGCTGATAGATTTGCCTGTCTAGTTGTTTTTACAGGGAgatgatga                                                                                                                   >  1:2711668/1‑140 (MQ=255)

                     catGAAGAGAAAGCGGCGCTGACCTGCGGTGATGGGGCGTTTATTCGTGACGAGGCTAACATTACGCTGGTTGCCGATTCCCCACTGCGCGCTTTGCTGATAGATTTGCCTGTCTAGTTGTTTTTACAGGGAgatgatga                                                                                                                   >  1:3970479/1‑140 (MQ=255)

                      atGAAGAGCAAGCGGCGCTGACCTGCGGAGAGGGGGCGTTTAATCGTGACGAGGATAACATTACGCTGGTTGCCGAGTCCCCACTGCGCGCTTTGCTGATAGATTTGCCTGTCTAGATGTTTTAACAGGGAgatgatgat                                                                                                                  <  2:5358878/140‑1 (MQ=255)

                      atGAAGAGAAAGCGGCGCTGACCTGCGGTGATGGGGCGTTTATTCGTGACGAGGCTAACATTACGCTGGTTGCCGATTCCCCACTGCGCGCTTTGCTGATAGATTTGCCTGTCTAGTTGTTTTTACAGGGAgatgatgat                                                                                                                  <  2:3880210/140‑1 (MQ=255)

                      atGAAGAGAAAGCGGCGCTGACCTGCGGTGATGGGGCGTTTATTCGTGACGAGGCTAACATTACGCTGGTTGCCGATTCCCCACTGCGCGCTTTGCTGATAGATTTGCCTGTCTAGTTGTTTTTACAGGGAgatgatgat                                                                                                                  >  2:3298149/1‑140 (MQ=255)

                       tGAAGAGAAAGCGGCGCTGACCTGCGGTGATGGGGCGTTTATTCGTGACGAGGCTAACATTACGCTGGTTGCCGATTCCCCACTGCGCGCTTTGCTGATAGATTTGCCTGTCTAGTTGTTTTTACAGGGAGatgatgatg                                                                                                                 >  1:2148679/1‑140 (MQ=255)

                           gagaAAGCGGCGCTGACCTGCGGTGATGGGGCGTTTATTCGTGACGAGGCTAACATTACGCTGGTTGCCGATTCCCCACTGCGCGCTTTGCTGATAGATTTGCCTGTCTAGTTGTTTTTACAGGGAGATGATGATGAGTa                                                                                                             >  1:3290526/1‑140 (MQ=255)

                           gagaAAGCGGCGCTGACCTGCGGTGATGGGGCGTTTATTCGTGACGAGGCTAACATTACGCTGGTTGCCGATTCCCCACTGCGCGCTTTGCTGATAGATTTGCCTGTCTAGTTGTTTTTACAGGGAGATGATGATGAGTa                                                                                                             >  1:101026/1‑140 (MQ=255)

                            agaAAGCGGCGCTGACCTGCGGTGATGGGGCGTTTATTCGTGACGAGGCTAACATTACGCTGGTTGCCGATTCCCCACTGCGCGCTTTGCTGATAGATTTGCCTGTCTAGTTGTTTTTACAGGGAGATGATGATGAGTaa                                                                                                            >  2:8917833/1‑140 (MQ=255)

                                  cggcgCTGACCTGCGGTGATGGGGCGTTTATTCGTGACGAGGCTAACATTACGCTGGTTGCCGATTCCCCACTGCGCGCTTTGCTGATAGATTTGCCTGTCTAGTTGTTTTTACAGGGAGATGATGATGAGTAAAAAAtt                                                                                                      <  1:4778688/140‑1 (MQ=255)

                                      gcTGACCTGCGGTGATGGGGCGTTTATTCGTGACGAGGCTAACATTACGCTGGTTGCCGATTCCCCACTGCGCGCTTTGCTGATAGATTTGCCTGTCTAGTTGTTTTTACAGGGAGATGATGATGAGTAAAAAATTGGcc                                                                                                  >  1:2965035/1‑140 (MQ=255)

                                         gACCTGCGGTGATGGGGCGTTTATTCGTGACGAGGCTAACATTACGCTGGTTGCCGAGTCCCCACTGCGCGCTTTGCTGATAGATTGGCCTGTCTAGTTGTTTTTACAGGGAGATGATGATGAGTAAAAAATTGGCCaaa                                                                                               <  2:5797798/140‑1 (MQ=255)

                                              gCGGTGATGGGGCGTTTATTCGTGACGAGGCTAACATTACGCTGGTTGCCGATTCCCCACTGCGCGCTTTGCTGATAGATTTGCCTGTCTAGTTGTTTTTACAGGGAGATGATGATGAGTAAAAAATTGGCCAAAAAgcg                                                                                          <  1:1092705/140‑1 (MQ=255)

                                               cGGTGATGGGGCGTTTATTCGTGACGAGGCTAACATTACGCTGGTTGCCGATTCCCCACTGCGCGCTTTGCTGATAGATTTGCCTGTCTAGTTGTTTTTACAGGGAGATGATGATGAGTAAAAAATTGGCCAAAAAgcgc                                                                                         >  1:4930990/1‑140 (MQ=255)

                                                ggTGCTGGGGCGTTTATTCGTGACGAGGCGAACATTACGCTGGTTGCCGATACCCCCCTGCGCGCTTTGCTGATAGATTTGCCTGTCTAGTTGTAATTACTGGGAGATGATGATGAGAAAAAAATTGGCCAAAAAGCGcc                                                                                        <  2:6038605/140‑1 (MQ=255)

                                                  ttaTGGGGCGTTTATTCGTGACGAGGCTAACATTACGCGTGTTGCCGATTCCCCACTGCGCTCTTTGCTGATAGATTTGCCTGTCTCGTTGTTTTTACAGGTATATGATGTTGCGTAAAAAATTGGCCAACACGCGCCAg                                                                                      <  2:2402826/138‑1 (MQ=255)

                                                    aTGGGGCGTTTATTCGTGACGAGGCTAACATTACGCTGGTTGCCGATTCCCCACTGCGCGCTTTGCTGATAGATTTGCCTGTCTAGTTGTTTTTACAGGGAGATGATGATGAGTAAAAAATTGGCCAAAAAGCGCCAGcc                                                                                    >  1:5339513/1‑140 (MQ=255)

                                                    aTGGGGCGTTTATTCGTGACGAGGCTAACATTACGCTGGTTGCCGATTCCCCACTGCGCGCTTTGCTGATAGATTTGCCTGTCTAGTTGTTTTTACAGGGAGATGATGATGAGTAAAAAATTGGCCAAAAAGCGCCAGcc                                                                                    >  1:1602212/1‑140 (MQ=255)

                                                    aTGGGGCGTTTATTCGTGACGAGGCTAACATTACGCTGGTTGCCGATTCCCCACTGCGCGCTTTGCTGATAGATTTGCCTGTCTAGTTGTTTTTACAGGGAGATGATGATGAGTAAAAAATTGGCCAAAAAGCGCCAGcc                                                                                    >  1:6908063/1‑140 (MQ=255)

                                                      ggggCGTTTATTCGTGACGAGGCTAACATTACGCTGGTTGCCGATTCCCCACTGCGCGCTTTGCTGATAGATTTGCCTGTCTAGTTGTTTTTACAGGGAGATGATGATGAGTAAAAAATTGGCCAAAAAGCGCCAGCCgg                                                                                  >  1:8634539/1‑140 (MQ=255)

                                                       gggCGTTTATTCGTGACGAGGCTAACATTACGCTGGTTGCCGATTCCCCACTGCGCGCTTTGCTGATAGATTTGCCTGTCTAGTTGTTTTTACAGGGAGATGATGATGAGTAAAAAATTGGCCAAAACGCGCCAGCCGGt                                                                                 <  1:7994829/140‑1 (MQ=255)

                                                        ggCGTTTATTCGTGACGAGGCTAACATTACGCTGGTTGCCGATTCCCCACTGCGCGCTTTGCTGATAGATTTGCCTGTCTAGTTGTTTTTACAGGGAGATGATGATGAGTAAAAAATTGGCCAAAAAGCGCCAGCCGGTg                                                                                <  1:8560686/140‑1 (MQ=255)

                                                        ggCGTTTATTCGTGACGAGGCTAACATTACGCTGGTTGCCGATTCCCCACTGCGCGCTGTGCTGATAGATTTGCCTGTCTAGTTGATTTTACAGGGAGATGATGATGAGTAAAAAATTGGCCAAAAAGCGCCAGCCGGTg                                                                                <  1:7724123/140‑1 (MQ=255)

                                                         gCGTTTATTCGTGACGAGGCTAACATTACGCTGGTTGCCGATTCCCCACTGCGCGCTTTGCTGATAGATTTGCCTGTCTAGTTGTTTTTACAGGGAGATGATGATGAGTAAAAAATTGGCCAAAAAGCGCCAGCCGGTGa                                                                               >  1:1854300/1‑140 (MQ=255)

                                                         gCGTTTATTCGTGACGAGGCTAACATTACGCTGGTTGCCGATTCCCCACTGCGCGCTTTGCTGATAGATTTGCCTGTCTAGTTGTTTTTACAGGGAGATGATGATGAGTAAAAAATTGGCCAAAAAGCGCCAGCCGGTGa                                                                               >  2:4740242/1‑140 (MQ=255)

                                                            tttATTCGTGACGAGGCTAACATTACGCTGGTTGCCGATTCCCCACTGCGCGCTTTGCTGATAGATTTGCCTGTCTAGTTGTTTTTACAGGGAGATGATGATGAGTACAAAATTGGCCAAAAAGCGCCAGCCGGTGAAGc                                                                            >  2:6565418/1‑140 (MQ=255)

                                                            tttATTCGTGACGAGGCTAACATTACGCTGGTTGCCGATTCCCCACTGCGCGCTTTGCTGATAGATTTGCCTGTCTAGTTGTTTTTACAGGGAGATGATGATGAGTAAAAATTTGGCCATAAAGCGCCCGCCGGTGAAGc                                                                            >  2:8289984/1‑140 (MQ=255)

                                                            tttATTCGTGACGAGGCTAACATTACGCTGGTTGCCGATTCCCCACTGCGCGCTTTGCTGATAGATTTGCCTGTCTAGTTGTTTTTACAGGGAGATGATGATGAGTAAAAAATTGGCCAAAAAGCGCCAGCCTGTGAAGc                                                                            >  2:8480203/1‑140 (MQ=255)

                                                            tttATTCGTGACGAGGCTAACATTACGCTGGTTGCCGATTCCCCACTGCGCGCTTTGCTGATAGATTTGCCTGTCTAGTTGTTTTTACAGGGAGATGATGATGAGTAAAAAATTGGCCAAAAAGCGCCAGCCGGTGAAGc                                                                            >  1:7867854/1‑140 (MQ=255)

                                                            tttATTCGTGACGAGGCTAACATTACGCTGGTTGCCGATTCCCCACTGCGCGCTTTGCTGATAGATTTGCCTGTCTAGTTGTTTTTACAGGGAGATGATGATGAGTAAAAAATTGGCCAAAAAGCGCCAGCCGGTGAAGc                                                                            >  1:6650263/1‑140 (MQ=255)

                                                              tATTCGTGACGAGGCTAACATTACGCTGGTTGCCGATTCCCCACTGCGCGCTTTGCTGATAGATTTGCCTGTCTAGTTGTTTTTACAGGGAGATGATGATGAGTAAAAAATTGGCCAAAAAGCGCCAGCCGGTGAAGccc                                                                          >  2:2762060/1‑140 (MQ=255)

                                                                ttCGTGACGAGGCTAACATTACGCTGGTTGCCGATTCCCCACTGCGCGCTTTGCTGATAGATTTGCCTGTCTAGTTGTTTTTACAGGGAGATGATGATGAGTAAAAAATTGGCCAAAAAGCGCCAGCCGGTGAAGCCCgt                                                                        <  1:6178675/140‑1 (MQ=255)

                                                                 tCGTGACGAGGCTAACATTACGCTGGTTGCCGATTCCCCACTGCGCGCTTTGCTGATAGATTTGCCTGTCTAGTTGTTTTTACAGGGAGATGATGATGAGTAAAAAATTGGCCAAAAAGCGCCAGCCGGTGAAGCCCgtg                                                                       <  1:738633/140‑1 (MQ=255)

                                                                   gTGACGAGGCGAACATTACGCTGGTTGCCGATTCCCCACTGCGCGCTTTGCTGATATATTTGCCTGTCTAGGTGAATTTACAGGGCGATGATGATGAGTAAAAAATTGGCCAAAAAGCGCCAGCCGCTGAAGCCCgtggt                                                                     <  2:291728/140‑1 (MQ=255)

                                                                      aCGAGGCTAACATTACGCTGGTTGCCGATTCCCCACTGCGCGCTTTGCTGATAGATTTGCCTGTCTAGTTGTTTTTACGGGGAGATGATGATGAGTAAAAAATTGGCCAAAAAGCGCCAGCCGGTGAAGCCCGTGGTGGc                                                                  >  2:4384716/1‑140 (MQ=255)

                                                                      aCGAGGCTAACATTACGCTGGTTGCCGATTCCCCACTGCGCGCTTTGCTGATAGATTTGCCTGTCTAGTTGTTTTTACAGGGAGATGATGATGAGTAAAAAATTGGCCAAAAAGCGCCAGCCGGTGAAGCCCGTGGTGGc                                                                  >  1:2203628/1‑140 (MQ=255)

                                                                                   tACGCTGGTTGCCGATTCCCCACTGCGCGCTTTGCTGATAGATTTGCCTGTCTAGTTGTTTTTACAGGGAGATGATGATGAGTAAAAAATTGGCCAAAAAGCGCCAGCCGGTGAAGCCCGTGGTGGCGAAAGAACCTGCt                                                     >  1:4291481/1‑140 (MQ=255)

                                                                                     cGCTGGTTGCCGATTCCCCACTGCGCGCTTTGCTGATAGATTTGCCTGTCTAGTTGTTTTTACAGGGAGATGATGATGAGTAAGAAATTGGCCAAAAAGCGCCAGCCGGTGAAGCCCGTGGTGGCGAAAGAACCTGCTCg                                                   >  2:7438806/1‑140 (MQ=255)

                                                                                     cGCTGGTTGCCGATTCCCCACTGCGCGCTTTGCTGATAGATTTGCCTGTCTAGTTGTTTTTACAGGGAGATGATGATGAGTAAAAAATTGGCCAAAAAGCGCCAGCCGGTGAAGCCCGTGGTGGCGAAAGAACCTGCTCg                                                   >  1:3086966/1‑140 (MQ=255)

                                                                                     cGCTGGTTGCCGATTCCCCACTGCGCGCTTTGCTGATAGATTTGCCTGTCTAGTTGTTTTTACAGGGAGATGATGATGAGTAAAAAATTGGCCAAAAAGCGCCAGCCGGTGAAGCCCGTGGTGGCGAAAGAACCTGCTCg                                                   >  2:5423420/1‑140 (MQ=255)

                                                                                     cGCTGGTTGCCGATTCCCCACTGCGCGCTTTGCTGATAGATTTGCCTGTCTAGTTGTTTTTACAGGGAGATGATGATGAGTAAAAAATTGGCCAAAAAGCGCCAGCCGGTGAAGCCCGTGGTGGCGAAAGAACCTGCTCg                                                   >  1:8231239/1‑140 (MQ=255)

                                                                                     cGCTGGTTGCCGATTCCCCACTGCGCGCTTTGCTGATAGATTTGCCTGTCTAGTTGTTTTTACAGGGAGATGATGATGAGTAAAAAATTGGCCAAAAAGCGCCAGCCGGTGAAGCCCGTGGTGGCGAAAGAACCTGCTCg                                                   >  1:5843354/1‑140 (MQ=255)

                                                                                     cGCTGGTTGCCGATTCCCCACTGCGCGCTTTGCTGATAGATTTGCCTGTCTAGTTGTTTTTACAGGGAGATGATGATGAGGAAAAAATTGGCCAAAAAGCGCCAGCCGGTGAAGCCCGTGGTGGCGAAAGAACCTGCTCg                                                   >  2:7568742/1‑140 (MQ=255)

                                                                                     cGCTGGTTGCCGATTCCCCACTGCGCGCTTTGCTGATAGATTTGCCTGGCTAGTTGTTTGTACAGGGAGATGAGGATGCGTCAAAAATTGGCCAAAAAGCGCCAGCCGGTGAAGCCCGGGGGGGCGAATGAACCTGCTCg                                                   >  2:357592/1‑140 (MQ=255)

                                                                                      gCTGGTTGCCGATTCCCCACTGCGCGCTTTGCTGATAGATTTGCCTGTCTAGTTGTTTTTa                                                                                                                                 <  1:5441561/61‑1 (MQ=255)

                                                                                        tGGTTGCCGATTCCCCACTGCGCGCTTTGCTGATAGATTTGCCTGTCTAGTTGTTTTTACAGGGAGATGATGATGAGTAAAAAATTGGCCAAAAAGCGCCAGCCGGTGAAGCCCGTGGTGGCGAAAGAACCTGCTCGCAc                                                <  2:1413479/140‑1 (MQ=255)

                                                                                         ggTTGCCGATTCCCCACTGCGCGCTTTGCTGATAGATTTGCCTGTCTAGTTGTTTTTACAGGGAGATGATGATGAGTCAAAAATTGGCCAAAAAGCGCCAGCCGGTGAAGCCCGTGGTGGCGAAAGAACCTGCTCGCAcc                                               >  1:1711175/1‑140 (MQ=255)

                                                                                          gTTGCCGATTCCCCACTGCGCGCTTTGCTGATAGATTTGCCTGTCTAGTTGTTTTTACAGGGAGATGATGATGAGTAAAAAATTGGCCAAAAAGCGCCAGCCGGTGAAGCCCGTGGTGGCGAAAGAACCTGCTCGCACCg                                              >  1:811632/1‑140 (MQ=255)

                                                                                          gTTGCCGATTCCCCACTGCGCGCTTTGCTGATAGATTTGCCTGTCTAGTTGTTTTTACAGGGAGATGATGATGAGTAAAAAATTGGCCAAAAAGCGCCAGCCGGTGAAGCCCGTGGTGGCGAAAGAACCTGCTCGCACCg                                              >  2:884388/1‑140 (MQ=255)

                                                                                          gTTGCCGATTCCCCACTGCGCGCTTTGCTGATAGATTTGCCTGTCTAGTTGTTTTTACAGGGAGATGATGATGAGTAAAAAATTGGCCAAAAAGCGCCAGCCGGTGAAGCCCGTGGTGGCGAAAGAACCTGCTCGCACCg                                              >  1:5273194/1‑140 (MQ=255)

                                                                                          gTTGCCGATTCCCCACTGCGCGCTTTGCTGATAGATTTGCCTGTCTAGTTGTTTTTACAGGGAGATGATGATGAGTAAAAAATTGGCCAAAAAGCGCCAGCCGGTGAAGCCCGTGGTGGCGAAAGAACCTGCTCGCACCg                                              >  1:9338484/1‑140 (MQ=255)

                                                                                          gTTGCCGATTCCCCACTGCGCGCTTTGCTGATAGATTTGCCTGTCTAGTTGTTTTTACAGGGAGATGATGATGAGTAAAAAATTGGCCAAAAAGCGCCAGCCGGTGAAGCCCGTGGTGGCGAAAGAACCTGCTCGCACCg                                              >  2:798203/1‑140 (MQ=255)

                                                                                           tagCCGATTCCCCACTGCGCGCTATGCTGATAGATTTGCCTGTCTAGTAGTTTTTACAGGGAGATGATGATGAGTAAAAAATTGGCCAAAAAGCTCCAGCCGGTGAAGCCCGTGGTGGCGAAAGAAACTGCTCGCACCGc                                             >  2:2491748/3‑140 (MQ=255)

                                                                                                    ccccACTGCGCGCTTTGCTGATAGATTTGCCTGTCTAGTTGTTTTTACAGGGAGATGATGATGAGTAAAAAATTGGCCAAAAAGCGCCAGCCGGTGAATCCCGTGGTGGCGAAAGAACCTGCTCGCACCGCCAAAAAttt                                    >  2:2650517/1‑140 (MQ=255)

                                                                                                    ccccACTGCGCGCTTTGCTGATAGATTTGCCTGTCTAGTTGTTTTTACAGGGAGATGATGATGAGTAAAAAATTGGCCAAAAAGCGCCAGCCGGTGAAGCCCGTGGTGGCGAAAGAACCTGCTCGCACCGCCAAAAAttt                                    >  2:1104541/1‑140 (MQ=255)

                                                                                                         cTGCGCGCTTTGCTGATAGATTTGCCTGTCTAGTTGTTTTTACAGGGAGATGATGATGAGTAAAAAATTGGCCAAAAAGCGCCAGCCGGTGAAGCCCGTGGTGGCGAAAGAACCTGCTCGCACCGCCAAAAATTTTGGCt                               >  1:8998611/1‑140 (MQ=255)

                                                                                                          tGCGCGCTTTGCTGATAGATTTGCCTGTCTAGTTGTTTTTACAGGGAGATGATGATGAGTAAAAAATTGGCCAAAAAGCGCCAGCCGGTGAAGCCCGTGGTGGCGAAAGAACCTGCTCGCACCGCCAAAAATTTTGGCTa                              <  2:3713028/140‑1 (MQ=255)

                                                                                                           gcgcgcTTTGCTGATAGATTTGCCTGTCTAGTTGTTTTTACAGGGAGATGATGATGAGTACAAAATTGGCCAAAAAGCGCCAGCCGGTGAAGCCCGTGGTGGCGTAAGAACCTGCTCGCACCGCCAAAAATTTTGGCTag                             >  1:8710871/1‑139 (MQ=255)

                                                                                                           gcgcgcTTTGCTGATAGATTTGCCTGTCTAGTTGTTTTTACAGGGAGATGATGATGAGTAAAAAATTGGCCAAAAAGCGCCAGCCGGTGAAGCCCGTGGTGGCGAAAGAACCTGCTCGCACCGCCAAAAATTTTGGCTAt                             <  2:6811027/140‑1 (MQ=255)

                                                                                                            cgcgcTTTGCTGATAGATTTGCCTGTCTAGTTGTTTTTACCGGGAGATGATGATGAGTAAAAAATTGGCCAAAAAGCGCCAGCCGGTGAAGCCCGTGGTGGCGAAAGAACCTGCTCGCACCGCCAAAAATATTGGCTATg                            >  2:8137778/1‑140 (MQ=255)

                                                                                                            cgcgcTTTGCTGATAGATTTGCCTGTCTAGTTGTTTTTACAGGGAGCTGATGATGAGTAAAAAATTGGCCAAAAAGCGCCAGCCGGTGAAGCCCGTGGTGGCGAAAGAACCTGNTCGCACCGCCAAAAATTTTGGCTATg                            >  1:7114288/1‑140 (MQ=255)

                                                                                                            cgcgcTTTGCTGATAGATTTGCCTGTCTAGTTGTTTTTACAGGGAGATGATGATGAGTAAAAAATTGGCCAAAAAGCGCCAGCCGGTGAAGCCCGTGGTGGCGAAAGAACCTGCTCGCACCGCCAAAAATTTTGGCTATg                            >  1:6982000/1‑140 (MQ=255)

                                                                                                            cgcgcTTTGCTGATAGATTTGCCTGTCTAGTTGTTTTTACAGGGAGATGATGATGAGTAAAAAATTGGCCAAAAAGCGCCAGCCGGTGAAGCCCGGGGTGGCGAAAGACACTGCTCGCACCGCCACAAATTTTGGCTATg                            >  2:2167047/1‑140 (MQ=255)

                                                                                                            cgcgcTTTGCTGATAGATTTGCCTGTCGAGTTGTTTTTACAGGGAGATGATGATGAGTAAAAAATTGGCCAAAAAGCGCCAGCCGGTGAAGCCCGTGGTGGCGAAAGAACCTGCTCGCACCGCCAAAAATTTTGGCTATg                            >  1:8086649/1‑140 (MQ=255)

                                                                                                              cgcTTTGCTGATAGATTTGCCTGTCTAGTTGTTTTTACAGGGAGATGATGATGAGTAAAAAATTGGCCAAAAAGCGCCAGCCGGTGAAGCCCGTGGTGGCGAAAGAACCTGCTCGCACCGCCAAAAATTTTGGCTATGaa                          <  2:4224963/140‑1 (MQ=255)

                                                                                                              cgcTTTGCTGATAGATTTGCCTGTCTAGTTGTTTTTACAGGGAGATGATGATGAGTAAAAAATTGGCCAAAAAGCGCCAGCCGGTGAAGCCCGTGGTGGCGAAAGAACCTGCTCGCACCGCCAAAAATTTTGGCTATGaa                          >  1:2491257/1‑140 (MQ=255)

                                                                                                               gcTTTGCTGATAGATTTGCCTGTCTAGTTGTTTTTACAGGGAGATGATGATGAGTAAAAAATTGGCCAAAAAGCGCCAGCCGGTGAAGCCCGTGGTGGCGAAAGAACCTGCTCGCACCGCCAAAAATTTTGGCTATGAag                         <  1:3749835/140‑1 (MQ=255)

                                                                                                                cTTTGCTGAGAGAGTTGCCTGTCTAGTTGTTGTTACAGGGAGATGATGATGAGTAAAAAATTGGCCAAAAAGCGCCAGCCGGTGAAGCCCGTGGTGGCGAAAGAACCTGCTCGCACCGCCAAAAATTTTGGCTATGAaga                        <  2:7284261/140‑1 (MQ=255)

                                                                                                                 ttttCTGATAGATTTGCCTGTAGAGTTGTATTTACAGGGAGAGGATGATGAGTAAAAAATTGGCCAAAAAGCGCCAGCCGGTGAAGCCCGTGGTGGCGAAAGAACCTGCTCGCACCGCCAAAAATTTTGGCTATGAagag                       <  2:1917806/140‑1 (MQ=255)

                                                                                                                 tttGCTGATAGATTTGCCTGTCTAGTTGTTTTTACAGGGAGATGATGATGAGTAAAAAATTGGCCAAAAAGCGCCAGCCGGTGAAGCCCGTGGTGGCGAAAGAACCTGCTCGCACCGCCAAAAATTTTGGCTATGAagag                       >  1:2116381/1‑140 (MQ=255)

                                                                                                                         tAGATTTGCCTGTCTAGTTGTTTTTACAGGGAGATGATGATGAGTAAAAAATTGGCCAAAAAGCGCCAGCCGGTGAAGCCCGTGGTGGCGAAAGAACCTGCTCGCACCGCCAAAAATTTTGGCTATGAAGAGATGTTgag               <  2:842216/140‑1 (MQ=255)

                                                                                                                         tAGATTTGCCTGTCTAGTTGTTTTTACAGGGAGATGATGATGAGTAAAAAATTGGCCAAAAAGCGCCAGCCGGTGAAGCCCGTGGTGGCGAAAGAACCTGCTCGCACCGCCAAAAATTTTGGCTATGAAGAGATGTTgag               <  1:7180642/140‑1 (MQ=255)

                                                                                                                         tAGATGTGCCTGTCTAGTTGTTTTTACAGGGAGATGATGAAGAGTAAAAAATTGGCCAAAAATCGCCAGCCTGTGAAGCAAGTGGTGGCGAAAGAACCTGCTCGCACCGCCAAAAATTTTGGCTATGAAGAGATTTTgag               <  2:1648143/140‑1 (MQ=255)

                                                                                                                          aGATTTGCCTGTCTAGTTGTTTTTACAGGGAGATGATGATGAGTAAAAAATTGGCCAAACAGCGCCAGCCGGTGAAGCCCGTGGTGGCGAAAGAACCTGCTCGCACCGCCAAAAATTTTGGCTATGAAGAGATGTTgagc              <  1:3515726/140‑1 (MQ=255)

                                                                                                                          aGATTTGCCTGTCTAGTTGTTTTTACAGGGAGATGATGATGAGTAAAAAATTGGCCAAAAAGCGCCAGCCGGTGAAGCCCGTGGTGGCGAAAGAACCTGCACGCACCGCCAAAAATTTTGGCTATGAAGAGATGTTgagc              <  2:5298726/140‑1 (MQ=255)

                                                                                                                           gATTTGCCTGTCTAGTTGTTTTTACAGGGAGATGATGATGAGTAAAAAATTGGCCAAAAAGCGCCAGCCGGTGAAGCCCGTGGTGGCGAAAGAACCTGCTCGCACCGCCAAAAATTTTGGCTATGAAGAGATGTTgagcg             >  2:3212086/1‑140 (MQ=255)

                                                                                                                           gATTTGCCTGTCTAGTTGTTTTTACAGGGAGATGATGATGAGTAAAAAATTGGCCAAAAAGCGCCAGCCGGTGAAGCCCGTGGTGGCGAAAGAACCTGCTCGCACCGCCAAAAATTTTGGCTATGAAGAGATGTTgagcg             >  1:8462621/1‑140 (MQ=255)

                                                                                                                             tttGCCTGTCTAGTTGTTTTTACAGGGAGATGATGATGAGTAAAAAATTGGCCAAAAAGCGCCAGCCGGTGAAGCCCGTGGTGGCGAAAGAACCTGCTCGCACCGCCAAAAATTTTGGCTATGAAGAGATGTTgagcgag           <  2:5627676/140‑1 (MQ=255)

                                                                                                                               tGCCTGTCTAGTTGTTTTTACAGGGAGATGATGATGAGTAAAAAATTGGCCAAAAAGCGCCAGCCGGTGAAGCCCGTGGTGGCGAAAGAACCTGCTCGCACCGCCAAAAATTTTGGCTATGAAGAGATGTTgag               >  2:9179998/1‑134 (MQ=255)

                                                                                                                               tGCCTGTCTAGTTGTTTTTACAGGGAGATGATGATGAGTAAAAAATTGGCCAAAAAGCGCCAGCCGGTGAAGCCCGTGGTGGCGAAAGAACCTGCTCGCACCGCCAAAAATTTTGGCTATGAAGAGATGTTgag               <  1:9179998/134‑1 (MQ=255)

                                                                                                                               tGCCTGTCTAGTTGTTTTTACAGGGAGATGATGATGAGTAAAAAATTGGCCAAAAAGCGCCAGCCGGTGAAGCCCGTGGTGGCGAAAGAACCTGCTCGCACCGCCAAAAATTTTGGCTATGAAGAGATGTTgag               >  2:7030851/1‑134 (MQ=255)

                                                                                                                               tGCCTGTCTAGTTGTTTTTACAGGGAGATGATGATGAGTAAAAAATTGGCCAAAAAGCGCCAGCCGGTGAAGCCCGTGGTGGCGAAAGAACCTGCTCGCACCGCCAAAAATTTTGGCTATGAAGAGATGTTGAGCGAGCt         <  1:7614892/140‑1 (MQ=255)

                                                                                                                               tGCCTGTCTAGTTGTTTTTACAGGGAGATGATGATGAGTAAAAAATTGGCCAAAAAGCGCCAGCCGGTGAAGCCCGTGGTGGCGAAAGAACCTGCTCGCACCGCCAAAAATTTTGGCTATGAAGAGATGTTGAGCGAGCt         >  1:5508287/1‑140 (MQ=255)

                                                                                                                                gCCTGTCTAGTTGTTTTTACAGGGAGATGATGATGAGTAAAAAATTGGCCAAAAAGCGCCAGCCGGTGAAGCCCGTGGTGGCGAAAGAACCTGCTCGCACCGCCCAAAATTTTGGCGATGAAGAGATGTTGAGCGAGCTg        >  2:6305181/1‑140 (MQ=255)

                                                                                                                                gCCTGTCTAGTTGTTTTTACAGGGAGATGATGATGAGTAAAAAATTGGCCAAAAAGCGCCAGCCGGTGAAGCCCGTGGTGGCGAAAGAACCTGCTCGCACCGCCAAAAATTTTGGCTATGAAGAGATGTTGAGCGAGCTg        <  1:7825087/140‑1 (MQ=255)

                                                                                                                                     tCTAGTTGTTTTTACAGGGAGATGATGATGAGTCACAACTTGGCCAAAAAGCGCCCGCCGGTGAAGCACGTGGTGGCGAATGAACCTGCTCGCACCGCCCAAAATTTGGGCGCGGAAGAGATGTTGAGCGAGCTGGAAGc   >  2:9425125/1‑140 (MQ=255)

                                                                                                                                      cTAGTTGTTTTTACAGGGAGATGATGATGAGTAAAAAATTGGCCAAAAAGCGCCAGCCGGTGAAGCCAGTGGTGGCGAAATAACCTGCTCGCACCGCCAAAAATTTTGGCTATTAAGAGATGTTGAGCGAGCTGGAAGCt  >  2:3046817/1‑140 (MQ=255)

AAATTTCATGCGCTTACGCATCATGAAGAGAAAGCGGCGCTGACCTGCGGTGATGGGGCGTTTATTCGTGACGAGGCTAACATTACGCTGGTTGCCGATTCCCCACTGCGCGCTTTGCTGATAGATTTGCCTGTCTAATTGTTTTTACAGGGAGATGATGATGAGTAAAAAATTGGCCAAAAAGCGCCAGCCGGTGAAGCCCGTGGTGGCGAAAGAACCTGCTCGCACCGCCAAAAATTTTGGCTATGAAGAGATGTTGAGCGAGCTGGAAGCT  >  NZ_CP025268/3255568‑3255841

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`