BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP025268	345,338	C→T	L17L (TTG→TTA)	yahN ←	LysE family transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP025268	345,338	0	C	T	99.6%	1731.0 / NA	441	L17L (TTG→TTA)	yahN	LysE family transporter
Reads supporting (aligned to +/- strand): ref base C (0/0); major base T (247/192); minor base G (2/0); total (249/192)
Fisher's exact test for biased strand distribution p-value = 5.07e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 441 total aligned reads displayed.

CCCAGCGCCACGCCCAGCCCGGTCAGCACCCCTGCGCGTCGACCGGAAGCCAGGCTGGTTTGTACTACCACAAAGAGATTGGCTCCCGGATTAAAAAAAGTAATCACGAACAGTCCTACGGTCAGGTAAACGGCATGCAAAGGATCCATAGTGATTTCATCCATAAATAAGTGAACTAACTGCATCATATTTCTACCAAAAATAATCGGGTGCGAGAGAGATCACAAAGTGTCTTATTTCCGGTTACTGGCGTTTATGCCCTGACTGAACTAAT  >  NZ_CP025268/345201‑345474

cccAGCGCCACGCCCAGCCCGGTCAGCACCCCTGCGCGTCGACCGGAAGCCAGGCTGGTTTGTACTACCACAAAGAGATTGGCTCCCGGATTAAAAAAAGTAATCACGAACAGTCCTACGGTCAGGTAAACGGCATGTaa                                                                                                                                        <  1:5180489/140‑1 (MQ=255)

cccAGCGCCACGCCCAACCCGGTCAGCACCCCTGCGCGTCGACCGGAAGCCAGGCTGGTTTGTACTACCACAAAGAGATTGGCTCCCGGATTAAAAAAAGTAATCACGAACAGTCCTACGGTCAGGTAAACGGCATGTaa                                                                                                                                        <  1:2564759/140‑1 (MQ=255)

  cAGCGCCACGCCCAGCCCGGTCAGCACCCCTGCGCGTCGACCGGAAGCCAGGCTGGTTTGTACTACCACAAAGAGATTGGCTCCCGGATTAAAAAAAGTAATCACGAACAGTCCTACGGTCAGGTAAACGGCATGTAAAg                                                                                                                                      <  2:5136690/140‑1 (MQ=255)

    gcgcCACGCCCAGCCCGGTCAGCACCCCTGCGCGTCGACCGGAAGCCAGGCTGGTTTGTACTACCACAAAGAGATTGGCTCCCGGATTAAAAAAAGTAATCACGAACAGTCCTACGGTCAGGTAAACGGCATGTAAAGGa                                                                                                                                    >  1:5915402/1‑140 (MQ=255)

     cgcCACGCCCAGCCCGGTCAGCACCCCTGCGCGTCGACCGGAAGCCAGGCTGGTTTGTACTACCACAAAGAGATTGGCTCCCGGATTAAAAAAAGTAATCACGAACAGTCCTACGGTCAGGTAAACGGCATGTAAAGGAt                                                                                                                                   >  2:2435344/1‑140 (MQ=255)

     cgcCACGCCCAGCCCGGTCAGCACCCCTGCGCGTCGACCGGAAGCCAGGCTGGTTTGTACTACCACAAAGAGATTGGCTCCCGGATTAAAAAAAGTAATCACGAACAGTCCTACGGTCAGGTAAACGGCATGTAAAGGAt                                                                                                                                   <  2:1333916/140‑1 (MQ=255)

     cgcCACGCCCAGCCCGGTCAGCACCCCTGCGCGTCGACCGGAAGCCAGGCTGGTTTGTACTACCACAAAGAGATTGGCTCCCGGATTAAAAAAAGTAATCACGAACAGTCCTACGGTCAGGTAAACGGCATGTAAAGGAt                                                                                                                                   >  1:7096884/1‑140 (MQ=255)

          cGCCCAGCCCGGTCAGCACCCCTGCGCGTCGACCGGAAGCCAGGCTGGTTTGTACTACCACAAAGAGATTGGCTCCCGGATTAAAAAAAGTAATCACGAACAGTCCTACGGTCAGGTAAACGGCATGTAAAGGATCCATa                                                                                                                              >  2:1490783/1‑140 (MQ=255)

                 cccTGTAAGCACCCCTGCGAGTCGTCCGGAAGCCAGGCTGGTTAGTACTCCCACAATGAGATTGTCTCACGGATAAAAAAAAGTAATCACTAACTGTCCTACGGTCAGGTAAAAGGAATGTAATGGATCCATAGTGAgtt                                                                                                                       <  2:8647058/140‑1 (MQ=255)

                 cccGGTCAGCACCCCTGCGCGTCGACCGGAAGCCAGGCTGGTTTGTACTACCACAAAGAGATTGGCTCCCGGATTAAAAAAAGTAATCACGAACAGTCCTACGGTCAGGTAAACGGCATGTAAAGGATCCATAGTGAttt                                                                                                                       <  1:6806651/140‑1 (MQ=255)

                 cccGGTCAGCACCCCTGCGCGTCGACCGGAAGCCAGGCTGGTTTGTACTACCACAAAGAGATTGGCTCCCGGATTAAAAAAAGTAATCACGAACAGTCCTACGGTCAGGTAAACGGCATGTAAAGGATCCATAGTGAttt                                                                                                                       <  1:6378162/140‑1 (MQ=255)

                    ggTCAGCACCCCTGCGCGTCGACCGGAAGCCAGGCTGGTTTGTACTACCACAAAGAGATTGGCTCCCGGATTAAAAAAAGTGATCACGAACAGTCCTACGGTCAGGTAAACGGCATGTAAAGGATCCATAGTGATTTCAt                                                                                                                    <  2:1573692/140‑1 (MQ=255)

                     gTCAGCACCCCTGCGCGTCGACCGGAAGCCAGGCTGGTTTGTACTACCACAAAGAGATTGGCTCCCGGATTAAAAAAAGTAATCACGAACAGTCCTACGGTCAGGTAAACGGCATGTAAAGGATCCATAGTGATTTCATc                                                                                                                   <  2:2846041/140‑1 (MQ=255)

                         gCACCCCTGCGCGTCGACCGGAAGCCAGGCTGGTTTGTACTACCACAAAGAGATTGGCTCCCGGATTAAAAAAAGTAATCACGAACAGTCCTGCGGTCAGGTAAACGGCATGTAAAGGATCCATAGTGATTTCATCCata                                                                                                               <  2:3118212/140‑1 (MQ=255)

                           aCCCCTGCGCGTCGACCGGAAGCCAGGCTGGTTTGTACTACCACAAAGAGATTGGCTCCCGGATTAAAAAAAGTAATCACGAACAGTCCTACGGTCAGGTAAACGGCATGTAAAGGATCCATAGTGATTTCATCCataaa                                                                                                             >  2:8789142/1‑140 (MQ=255)

                             cccTGCGCGTCGTCCGGAAGCCAGGCTGGTTTGTACTACCACAAAGAGATTGTCTCCCGGATTAAAAAAAGTAATCACGAACAGTCCTACGGTCAGGTAAACGGCATGTAAAGGATCCATAGTGTTTTCATCCataaata                                                                                                           <  1:9291317/140‑1 (MQ=255)

                             cccTGCGCGTCGACCGGAAGCCAGGCTGGTTTGTACTACCACAAAGAGATTGGCTCCCGGATTAAAAAAAGTAATCACGAACAGTCCTACGGTCAGGTAAACGGCATGTAAAGGATCCATAGTGATTTCATCCataaata                                                                                                           <  2:4553540/140‑1 (MQ=255)

                             cccTGCGCGTCGACCGGAAGCCAGGCTGGTTTGTACTACCACAAAGAGATTGGCTCCCGGATTAAAAAAAGTAATCACGAACAGTCCTACGGTCAGGTAAACGGCATGTAAAGGATCCATAGTGATTTCATCCataaata                                                                                                           <  1:4165195/140‑1 (MQ=255)

                               cTGCGCGTCGACCGGAAGCCAGGCTGGTTTGTACTACCACAAAGAGATTGGCTCCCGGATTAAAAAAAGTAATCACGAACAGTCCTACGGTCAGGTAAACGGCATGTAAAGGATCCATAGTGATTTCATCCATAAATAAg                                                                                                         >  1:8867873/1‑140 (MQ=255)

                                tGCGCGTCGACCGGAAGCCAGGCTGGTTTGTACTACCACAAAGAGATTGGCTCCCGGATTAAAAAAAGTAATCACGAACAGTCCTACGGTCAGGTAAACGGCATGTAAAGGATCCATAGTGATTTCATCCATAAATAAGt                                                                                                        <  1:397441/140‑1 (MQ=255)

                                tGCGCGTCGACCGGAAGCCAGGCTGGTTTGTACTACCACAAAGAGATTGGCTCCCGGATTAAAAAAAGTAATCACGAACAGTCCTACGGTCAGGTAAACGGCATGTAAAGGATCCATAGTGATTTCATCCATAAATAAGt                                                                                                        >  1:2893952/1‑140 (MQ=255)

                                 gcgcGTCGACCGGAAGCCAGGCTGGTTTGTACTACCACAAAGAGATTGGCTCCCGGATTAAAAAAAGTAATCACGAACAGTCCTACGGTCAGGTAAACGGCATGTAAAGGATCCATAGTGATTTCATCCATAAATAAGTg                                                                                                       <  2:4288600/140‑1 (MQ=255)

                                    cgTCGACCGGAAGCCAGGCTGGTTTGTACTACCACAAAGAGATTGGCTCCCGGATTAAAAAAAGTAATCACGAACAGTCCTACGGTCAGGTAAACGGCATGTAAAGGATCCATAGTGATTTCATCCATAAATAAGTGaac                                                                                                    >  1:4984227/1‑140 (MQ=255)

                                     gTCGACCGGAAGCCAGGCTGGTTTGTACTACCACAAAGAGATTGGCTCCCGGATTAAAAAAAGTAATCACGAACAGTCCTACGGTCAGGTAAACGGCATGTAAAGGATCCATAGTGATTTCATCCATAAATAAGTGaact                                                                                                   <  2:3879821/140‑1 (MQ=255)

                                     gTCGACCGGAAGCCAGGCTGGTTTGTACTACCACAAAGAGATTGGCTCCCGGATTAAAAAAAGTAATCACGAACAGTCCTACGGTCAGGTAAACGGCATGTAAAGGATCCATAGTGATTTCATCCATAAATAAGTGaact                                                                                                   <  1:650438/140‑1 (MQ=255)

                                       cGACCGGAAGCCAGGCTGGTTTGTACTACCACAAAGAGATTGGCTCCCGGATTAAAAAAAGTAATCACGAACAGTCCTACGGTCAGGTAAACGGCATGTAAAGGATCCATAGTGATTTCATCCATAAATAAGTGaactaa                                                                                                 <  2:4410823/140‑1 (MQ=255)

                                          ccGGAAGCCAGGCTGGTTTGTACTACCACACAGAGCTTGGCTCCCGGATTAAAAAAAGTAATCACGAACAGTCCTACGGTCAGGTAAACGGCATGTAAAGGATCCATAGTGATTTCATCCATAACTAAGTGAACTAACTg                                                                                              <  2:7264545/140‑1 (MQ=255)

                                          ccGGAAGCCAGGCTGGTTTGTACTACCACAAAGAGATTGGCTCCCGGATTAAAAAAAGTAATCACGAACAGTCCTACGGTCAGGTAAACGGCATGTAAAGGATCCATAGTGATTTCATCCATAAATAAGTGAACTAACTg                                                                                              <  2:9382903/140‑1 (MQ=255)

                                          ccGGAAGCCAGGCTGGTTTGTAATACCACAACGATATGGGCTCCCGGATTAAAATAAGTAATCCCGAACAGTCCTACGGTCAGGTAAACGGCATGTAAAGGATCCATAGTGATTTCATCCATAAATAAGTGAACTAACTg                                                                                              <  2:6929735/140‑1 (MQ=255)

                                               aGCCAGGCTGGTTTGTACTACCACAAAGAGATTGGCTCCCGGATTAAAAAAAGTAATCACGAACAGTCCTACGGTCAGGTAAACGGCATGTAAAGGATCCATAGTGATTTCATCCATAAATAAGTGAACTAACTGcatca                                                                                         <  1:5706158/140‑1 (MQ=255)

                                                  cAGGCTGGTTTGTACTACCACAAAGAGATTGGCTCCCGGATTAAAAAAAGTAATCACGAACAGTCCTACGGTCAGGTAAACGGCATGTAAAGGATCCATAGTGATTTCATCCATAAATAAGTGAACTAACTGCATCatat                                                                                      >  1:1722825/1‑140 (MQ=255)

                                                    ggCTGGTTTGTACTACCACAAAGAGATTGGCTCCCGGATTAAAAAAAGTAATCACGAACAGTCCTACGGTCAGGTAAACGGCATGTAAAGGATCCATAGTGATTTCATCCATAAATAAGTGAACTAACTGCATCATAttt                                                                                    >  1:7011446/1‑140 (MQ=255)

                                                       tGGTTTGTACTACCACAAAGAGATTGGCTCCCGGATTAAAAAAAGTAATCACGAACAGTCCTACGGTCAGGTAAACGGCATGTAAAGGATCCATAGTGATTTCATCCATAAATAAGTGAACTAACTGCATCATATTTCTa                                                                                 <  1:8482545/140‑1 (MQ=255)

                                                         gTTTGTACTACCACACAGAGACTGGCTCCCGGATTAAAAAAAGTAATCACGAACAGTCCTACGGTCAGGTAAACGGCATGTAAAGGATCCATAGTGATTTCATCCATAAATAAGTGAACTAACTGCATCATATTTCTAcc                                                                               <  2:6737862/140‑1 (MQ=255)

                                                         gTTTGTACTACCACAAAGAGATTGGCTCCCGGATTAAAAAAAGTAATCACGAACAGTCCTACGGTCAGGTAAACGGCATGTAAAGGATCCATAGTGATTTCATCCATAAATAAGTGAACTAACTGCATCATATTTCTAcc                                                                               <  1:1174613/140‑1 (MQ=255)

                                                              tactacCACAAAGAGATTGGCTCCCGGATTAAAAAAAGTAATCACGAACAGTCCTACGGTCAGGTAAACGGCATGTAAAGGATCCATAGTGATTTCATCCATAAATAAGTGAACTAACTGCATCATATTTCTACCaaaaa                                                                          >  2:324123/1‑140 (MQ=255)

                                                               actacCACAAAGAGATTGGCTCCCGGATTAAAAAAAGTAATCACGAACAGTCCTACGGTCAGGTAAACGGCATGTAAAGGATCCATAGTGATTTCATCCATAAATAAGTGAACTAACTGCATCATATTTCTACCAAAaat                                                                         >  1:7539856/1‑140 (MQ=255)

                                                                   ccACAAAGAGATTGGCTCCCGGATTAAAAAAAGTAATCACGAACAGTCCTACGGTCAGGTAAACGGCATGTAACGGATCCATAGTGATTTCATCCATAAATAAGTGAACTAACTGCATCATATTTCTACCAAAAATAATc                                                                     <  1:5108541/140‑1 (MQ=255)

                                                                   ccACAAAGAGATTGGCTCCCGGATTAAAAAAAGTAATCACGAACAGTCCTACGGTCAGGTAAACGGCATGTAAAGGATCCATAGTGATTTCATCCATAAATAAGTGAACTAACTGCATCATATTTCTACCAAAAATAATc                                                                     >  1:1010886/1‑140 (MQ=255)

                                                                   ccACAAAGAGATTGGCTCCCGGATTAAAAAAAGTAATCACGAACAGTCCTACGGTCAGGTAAACGGCATGTAAAGGATCCATAGTGATTTCATCCATAAATAAGTGAACTAACTGCATCATATTTCTACCAAAAATAATc                                                                     >  2:2128509/1‑140 (MQ=255)

                                                                   ccACAAAGAGATTGGCTCCCGGATTAAAAAAAGTAATCACGAACAGTCCTACGGTCAGGTAAACGGCATGTAAAGGATCCATAGTGATTTCATCCATAAATAAGTGAACTAACTGCATCATATTTCTACCAAAAATAATc                                                                     <  2:5979911/140‑1 (MQ=255)

                                                                   ccACAAAGAGATTGGCTCCCGGATTAAAAAAAGTAATCACGAACAGTCCTACGGTCAGGTAAACAGCATGTAAAGGATCCATAGTGATTTCATCCATAAATAAGTGAACTAACTGCATCATATTTCTACCAAAAATAATc                                                                     <  2:7157734/140‑1 (MQ=255)

                                                                      caAAGAGATTGGCTCCCGGATTAAAAAAAGTAATCACGAACAGTCCTACGGTCAGGTAAACGGCATGTAAAGGATCCATAGTGATTTCATCCATAAATAAGTGaact                                                                                                   >  2:7824855/1‑107 (MQ=255)

                                                                      caAAGAGATTGGCTCCCGGATTAAAAAAAGTAATCACGAACAGTCCTACGGTCAGGTAAACGGCATGTAAAGGATCCATAGTGATTTCATCCATAAATAAGTGaact                                                                                                   <  1:7824855/107‑1 (MQ=255)

                                                                      caAAGAGATTGGCTCCCGGATTAAAAAAAGTAATCACGAACAGTCCTACGGTCAGGTAAACGGCATGTAAAGGATCCATAGTGATTTCATCCATAAATAAGTGAACTAACTGCATCATATTTCTACCAAAAATAATCggg                                                                  >  1:6332238/1‑140 (MQ=255)

                                                                      caAAGAGATTGGCTCCCGGATTAAAAAAAGTAATCACGAACAGTCCTACGGTCAGGTAAACGGCATGTAAAGGATCCATAGTGATTTCATCCATAAATAAGTGAACTAACTGCATCATATTTCTACCAAAAATAATCggg                                                                  >  1:3890685/1‑140 (MQ=255)

                                                                        aaGAGATTGGCTCCCGGATTAAAAAAAGTAATCACGAACAGTCCTACGGTCAGGTAAACGGCATGTAAAGGATCCATAGTGATTTCATCCATAAATAAGTGAACTAACTGCATCATATTTCTACCAAAAATAATCGGGTg                                                                >  1:6076689/1‑140 (MQ=255)

                                                                        aaGAGATTGGCTCCCGGATTAAAAAAAGTAATCACGAACAGTCCTACGGTCAGGTAAACGGCATGTAAAGGATCCATAGTGATTTCATCCATAAATAAGTGAACTAACTGCATCATATTTCTACCAAAAATAATCGGGTg                                                                >  1:2703468/1‑140 (MQ=255)

                                                                        aaGAGATTGGCTCCCGGATTAAAAAAAGTAATCACGAACAGTCCTACGGTCAGGTAAACGGCATGTAAAGGATCCATAGTGATTTCATCCATAAATAAGTGAACTAACTGCATCATATTTCTACCAAAAATAATCGGGTg                                                                >  2:3438854/1‑140 (MQ=255)

                                                                        aaGAGATTGGCTCCCGGATTAAAAAAAGTAATCACGAACAGTCCTACGGTCAGGTAAACGGCATGTAAAGGATCCATAGTGATTTCATCCATAAATAAGTGAACTAACTGCATCATATTTCTACCAAAAATAATCGGGTg                                                                >  2:6603747/1‑140 (MQ=255)

                                                                           agaTTGGCTCCCGGATTAAAAAAAGTAATCACGAACAGTCCTACGGTCAGGTAAACGGCATGTAAAGGATCCATAGTGATTTCATCCATAAATAAGTGAACTAACTGCATCATATTTCTACCAAAAATAATCGGGTGCga                                                             >  2:6099464/1‑140 (MQ=255)

                                                                             aTTGGCTCCCGGATTAAAAAAAGTAATCACGAACAGTCCTACGGTCAGGTAAACGGCATGTAAAGGATCCATAGTGATTTCATCCATAAATAAGTGaactaa                                                                                                 >  1:4442914/1‑102 (MQ=255)

                                                                             aTTGGCTCCCGGATTAAAAAAAGTAATCACGAACAGTCCTACGGTCAGGTAAACGGCATGTAAAGGATCCATAGTGATTTCATCCATAAATAAGTGAACTAACTGCATCATATTTCTACCAAAAATAATCGGGTGCgaga                                                           >  1:947793/1‑140 (MQ=255)

                                                                             aTTGGCTCCCGGATTAAAAAAAGTAATCACGAACAGTCCTACGGTCAGGTAAACGGCATGTAAAGGATCCATAGTGATTTCATCCATAAATAAGTGAACTAACTGCATCATATTTCTACCAAAAATAATCGGGTGCgaga                                                           >  2:9069455/1‑140 (MQ=255)

                                                                             aTTGGCTCCCGGATTAAAAAAAGTAATCACGAACAGTCCTACGGTCAGGTAAACGGCATGTAAAGGATCCATAGTGATTTCATCCATAAATAAGTGAACTAACTGCATCATATTTCTACCAAAAATAATCGGGTGCgaga                                                           >  1:3204104/1‑140 (MQ=255)

                                                                             aTTGGCTCCCGGATTAAAAAAAGTAATCACGAACAGTCCTACGGTCAGGTAAACGGCATGTAAAGGATCCATAGTGATTTCATCCATAAATAAGTGAACTAACTGCATCATATTTCTACCAAAAATAATCGGGTGCgaga                                                           >  1:3483422/1‑140 (MQ=255)

                                                                             aTTGGCTCCCGGATTAAAAAAAGTAATCACGAACAGTCCTACGGTCAGGTAAACGGCATGTAAAGGATCCATAGTGATTTCATCCATAAATAAGTGAACTAACTGCATCATATTTCTACCAAAAATAATCGGGTGCgaga                                                           >  2:7384985/1‑140 (MQ=255)

                                                                              ttGGCTCCCGGAATAAAAAAAGTAATCACGAACAGTCCTACGGTCAGGTAAACGTCAAGTAAAGGATCCATAGTGATTTCATCCATAAATAAGTGAACTAACTGCATCATATTTCTACCAAAAATAATCGGGTGCgagag                                                          <  2:6406537/140‑1 (MQ=255)

                                                                              atgtcTCCCTGATTAAAAAAAGTAATCACGAACATTCCTCCGGTCAGGTACACGGCATGTAAAGGATCCATATTGATTTCAGCCATCAAAAAGTCACCTAACTGCATCAGATTTCTACCAAAAATAATCGGGTGCgagag                                                          <  2:1941093/136‑1 (MQ=255)

                                                                                 gCTCCCGGATTAAAAAAAGTAATCACGAACAGTCCTACGGTCAGGTAAACGGCATGTAAAGGATCCATAGTGATTTCATCCATAAATAAGTGAACTAACTGCATCATATTTCTACCAAAAATAATCGGGTGCgagagaga                                                       >  2:8387998/1‑140 (MQ=255)

                                                                                    cccGGATTAAAAAAAGTAATCACGAACAGTCCTACGGTCAGGTAAACGGCATGTAAAGGATCCATAGTGATTTCATCCATAAATAAGTGAACTAACTGCATCATATTTCTACCAAAAATAATCGGGTGCGAGAGAGATca                                                    <  1:4677251/140‑1 (MQ=255)

                                                                                      cGGATTAAAAAAAGTAATCACGAACAGTCCTACGGTCAGGTAAACGGCATGTAAAGGATCCATAGTGATTTCATCCATAAATAAGTGAACTAACTGCATCATATTTCTACCAAAAATAATCGGGTGCGAGAGAGATcaca                                                  <  1:5318206/140‑1 (MQ=255)

                                                                                        gATTAAAAAAAGTAATCACGAACAGTCCTACGGTCAGGTAAACGGCATGTAAAGGATCCATAGTGATTTCATCCATAAATAAGTGAACTAACTGCATCATATTTCTACCAAAAATAATCGGGTGCGAGAGAGATCACaaa                                                <  1:7191446/140‑1 (MQ=255)

                                                                                                  aGTAATCACGAACAGTCCTACGGTCAGGTAAACGGCATGTAAAGGATCCATAGTGATTTCATCCATAAATAAGTGAACTAACTGCATCATATTTCTACCAAAAATAATCGGGTGCGAGAGAGATCACAAAGTGTCTTAtt                                      >  2:8063260/1‑140 (MQ=255)

                                                                                                   gTAATCACGAACAGTCCTACGGTCAGGTAAACGGCATGTAAAGGATCCATAGTGATTTCATCCATAAATAAGTGAACTAACTGCATCATATTTCTACCAAAAATAATCGGGTGCGAGAGAGATCACAAAGTGTCTTAttt                                     <  2:5882434/140‑1 (MQ=255)

                                                                                                   gTAATCACGAACAGTCCTACGGTCAGGTAAACGGCATGTAAAGGATCCATAGTGATTTCATCCATAAATAAGTGAACTAACTGCATCATATTTCTACCAAAAATAATCGGGTGCGAGAGAGATCACAAAGTGTCTTAttt                                     >  2:3652426/1‑140 (MQ=255)

                                                                                                       tCACGAACAGTCCTACGGTCAGGTAAACGGCATGTAAAGGATCCATAGTGATTTCATCCATAAATAAGTGAACTAACTGCATCATATTTCTACCAAAAATAATCGGGTGCGAGAGAGATCACAAAGTGTCTTATTTCCgg                                 >  2:9192681/1‑140 (MQ=255)

                                                                                                       tCACGAACAGTCCTACGGTCAGGTAAACGGCATGTAAAGGATCCATAGTGATTTCATCCATAAATAAGTGAACTAACTGCATCATATTTCTACCAAAAATAATCGGGTGCGAGAGAGATCACAAAGTGTCTTATTTCCgg                                 >  2:5755987/1‑140 (MQ=255)

                                                                                                            aaCAGTCCTACGGTCAGGTAAACGGCATGTAAAGGATCCATAGTGATTTCATCCATAAATAAGTGAACTAACTGCATCATATTTCTACCAAAAATAATCGGGTGCGAGAGAGATCACAAAGTGTCTTATTTCCGGTTACt                            <  1:8189436/140‑1 (MQ=255)

                                                                                                            aaCAGTCCTACGGTCAGGTAAACGGCATGTAAAGGATCCATAGTGATTTCATCCATAAATAAGTGAACTAACTGCATCATATTTCTACCAAAAATAATCGGGTGCGAGAGAGATCACAAAGTGTCTTATTTCCGGTTACt                            <  1:1974442/140‑1 (MQ=255)

                                                                                                              cAGTCCTACGGTCAGGTAAACGGCATGTAAAGGATCCATAGTGATTTCATCCATAAATAAGTGAACTAACTGCATCATATTTCTACCAAAAATAATCGGGTGCGAGAGAGATCACAAAGTGTCTTATTTCCGGTTACTgg                          >  2:5673785/1‑140 (MQ=255)

                                                                                                              cAGTCCTACGGTCAGGTAAACGGCATGTAAAGGATCCATAGTGATTTCATCCATAAATAAGTGAACTAACTGCATCATATTTCTACCAAAAATAATCGGGTGCGAGAGAGATCACAAAGTGTCTTATTTCCGGTTACTgg                          <  1:3748730/140‑1 (MQ=255)

                                                                                                                 tCCTACGGTCAGGTAAACGGCATGTAAAGGATCCATAGTGATTTCATCCATAAATAAGTGAACTTACTGCATCATATTTCTACCAAAAATAATCGGGTGCGAGAGAGATCACAAAGTGTCTTATTTCCGGTTACTGGCGt                       >  1:3328304/1‑140 (MQ=255)

                                                                                                                 tCCTACGGTCAGGTAAACGGCATGTAAAGGATCCATAGTGATTTCATCCATAAATAAGTGAACTAACTGCATCATATTTCTACCAAAAATAATCGGGTGCGAGAGAGATCACAAAGTGTCTTATTTCCGGTTACTGGCGt                       >  1:617970/1‑140 (MQ=255)

                                                                                                                 tCCTACGGTCAGGTAAACGGCATGTAAAGGATCCATAGTGATTTCATCCATAAATAAGTGAACTAACTGCATCATATTTCTACCAAAAATAATCGGGTGCGAGAGAGATCACAAAGTGTCTTATTTCCGGTTACTGGCGt                       >  1:5672294/1‑140 (MQ=255)

                                                                                                                 tCCAACGGTCAGGTAAACGGCATGTAAAGGATCCATAGTGATTTCATCCATAAATAAGTGAACTAACTGCATCATATTTCTACCAAAAATAATCGGGTGCGAGAGAGATCACAAAGTGTCTTATTTCCGGTTACTGGCGt                       >  1:2462950/1‑140 (MQ=255)

                                                                                                                  ccTACGGTCAGGTAAACGGCATGTAAAGGATCCATAGTGATTTCATCCATAAATAAGTGAACTAACTGCATCATATTTCTACCAAAAATAATCGGGTGCGAGAGAGATCACAAAGTGTCTTATTTCCGGTTACTGGCGtt                      <  1:4352019/140‑1 (MQ=255)

                                                                                                                  ccTACGGTCAGGTAAACGGCATGTAAAGGATCCATAGTGATTTCATCCATAAATAAGTGAACTAACTGCATCATATTTCTACCAAAAATAATCGGGTGCGAGAGAGATCACAAAGTGTCTTATTTCCGGTTACTGGCGtt                      <  1:1505415/140‑1 (MQ=255)

                                                                                                                   cTACGGTCAGGTAAACGGCATGTAAAGGATCCATAGTGATTTCATCCATAAATAAGTGAACTAACTGCATCATATTTCTACCAAAAATAATCGGGTGCGAGAGAGATCACAAAGTGTCTTATTTCCGGTTACTGGCGttt                     <  2:2590549/140‑1 (MQ=255)

                                                                                                                     aCGGTCAGGTAAACGGCATGTAAAGGATCCATAGTGATTTCATCCATAAATAAGTGAACTAACTGCATCATATTTCTACCAAAAATAATCGGGTGCGAGAGAGATCACAAAGTGTCTTATTTCCGGTTACTGGCGTTTAt                   >  1:7709533/1‑140 (MQ=255)

                                                                                                                     aCGGTCAGGTAAACGGCATGTAAAGGATCCATAGTGATTTCATCCATAAATAAGTGAACTAACTGCATCATATTTCTACCAAAAATAATCGGGTGCGAGAGAGATCACAAAGTGTCTTATTTCCGGTTACTGGCGTTTAt                   >  1:1325138/1‑140 (MQ=255)

                                                                                                                     aCGGTCAGGTAAACGGCATGTAAAGGATCCATAGTGATTTCATCCATAAATAAGTGAACTAACTGCATCATATTTCTACCAAAAATAATCGGGTGCGAGAGAGATCACAAAGTGTCTTATTTCCGGTTACTGGCGTTTAt                   >  1:5411593/1‑140 (MQ=255)

                                                                                                                         tCAGGTAAACGGCATGTAAAGGATCCATAGTGATTTCATCCATAAATAAGTGAACTAACTGCATCATATTTCTACCAAAAATAATCGGGTGCGAGAGAGATcaca                                                  >  2:4927682/1‑105 (MQ=255)

                                                                                                                           aGGTAAACGGCATGTAAAGGATCCATAGTGATTTCAt                                                                                                                    <  2:4100542/37‑1 (MQ=255)

                                                                                                                           aGGTAAACGGCATGTAAAGGATCCATAGTGATTTCATCCATAAATAAGTGAACTAACTGCATCATATTTCTACCAAAAATAATCGGGTGCGAGAGAGATCACAAAGTGTCTTATTTCCGGTTACTGGCGTTTATGCCctg             >  1:7603440/1‑140 (MQ=255)

                                                                                                                           aGGTAAACGGCATGTAAAGGATCCATAGTGATTTCATCCATAAATAAGTGAACTAACTGCATCATATTTCTACCAAAAATAATCGGGTGCGAGAGAGATCACAAAGTGTCTTATTTCCGGTTACTGGCGTTTATGCCctg             >  2:760659/1‑140 (MQ=255)

                                                                                                                           aGGTAAACGGCATGTAAAGGATCCATAGTGATTTCATCCATAAATAAGTGAACTAACTGCATCATATTTCTACCAAAAATAATCGGGTGCGAGAGAGATCACAAAGTGTCTTATTTCCGGTTACTGGCGTTTATGCCctg             >  2:3033023/1‑140 (MQ=255)

                                                                                                                           aGGTAAACGGCATGTAAAGGATCCATAGTGATTTCATCCATAAATAAGTGAACTAACTGCATCATATTTCTACCAAAAATAATCGGGTGCGAGAGAGATCACAAAGTGTCTTATTTCCGGTTACTGGCGTTTATGCCctg             >  1:8053736/1‑140 (MQ=255)

                                                                                                                           aGGTAAACGGCATGTAAAGGATCCATAGTGATTTCATCCATAAATAAGTGAACTAACTGCATCATATTTCTACCAAAAATAATCGGGTGCGAGAGAGATCACAAAGTGTCTTATTTCCGGTTACTGGCGTTTATGCCctg             >  1:2174103/1‑140 (MQ=255)

                                                                                                                           aGGTAAACGGCATGTAAAGGATCCATAGTGATTTCATCCATAAATAAGTGAACTAACTGCATCATATTTCTACCAAAAATAATCGGGTGCGAGAGAGATCACAAAGTGTCTTATTTCCGGTTACTGGCGTTTATGCCctg             >  1:900057/1‑140 (MQ=255)

                                                                                                                           aGGTAAACGGCATGTAAAGGATCCATAGTGATTTCATCCATAAATAAGTGAACTAACTGCATCATATTTCTACCAAAAATAATCGGGTGCGAGAGAGATCACAAAGTGTCTTATTTCCGGTTACTGGCGTTTATGCCctg             >  2:5395008/1‑140 (MQ=255)

                                                                                                                           aGGTAAACGGCATGTAAAGGATCCATAGTGATTTCATCCATAAATAAGTGAACTAACTGCATCATATTTCTACCAAAAATAATCGGGTGCGAGAGAGATCACAAAGTGTCTTATTTCCGGTTACTGGCGTTTATGCCctg             >  1:2980546/1‑140 (MQ=255)

                                                                                                                                 aCGGCATGTAAAGGATCCATAGTGATTTCATCCATAAATAAGTGAACTAACTGCATCATATTTCTACCAAAAATAATCGGGTGCGAGAGAGATCACAAAGTGTCTTATTTCCGGTTACTGGCGTTTATGCCCTGACTGaa       <  1:9133749/140‑1 (MQ=255)

                                                                                                                                 aCGGCATGTAAAGGATCCATAGTGATTTCATCCATAAATAAGTGAACTAACTGCATCATATTTCTACCAAAAATAATCGGGTGCGAGAGAGATCACAAAGTGTCTTATTTCCGGTTACTGGCGTTTATGCCCTGACTGaa       <  2:4817705/140‑1 (MQ=255)

                                                                                                                                  cGGCATGTAAAGGATCCATAGTGATTTCATCCATAAATAAGTGAACTAACTGCATCATATTTCTACCAAAAATAATCGGGTGCGAGAGAGATCACAAAGTGTCTTATTTCCGGTTACTGGCGTTTATGCCCTGACTGAAc      >  1:6558780/1‑140 (MQ=255)

                                                                                                                                   ggCATGTAAAGGATCCATAGTGATTTCATCCATAAATAAGTGAACTAACTGCATCATATTTCTACCAAAAATAATCGGGTGCGAGAGAGATCACAAAGTGTCTTATTTCCGGTTACTGGCGTTTATGCCCTGACTGAACt     >  2:6499445/1‑140 (MQ=255)

                                                                                                                                   ggCATGTAAAGGATCCATAGTGATTTCATCCATAAATAAGTGAACTAACTGCATCATATTTCTACCAAAAATAATCGGGTGCGAGAGAGATCACAAAGTGTCTTATTTCCGGTTACTGGCGTTTATGCCCTGACTGAACt     <  2:6219546/140‑1 (MQ=255)

                                                                                                                                   ggCATGTAAAGGATCCATAGTGATTTCATCCATAAATAAGTGAACTAACTGCATCATATTTCTACCAAAAATAATCGGGTGCGAGAGAGATCACAAAGTGTCTTATTTCCGGTTACTGGCGTTTATGCCCTGACTGAACt     <  2:1697715/140‑1 (MQ=255)

                                                                                                                                     cATGTAAAGGATCCATAGTGATTTCATCCATAAATAAGTGAACTAACTGCATCATATTTCTACCAAAAATAATCGGGTGCGAGAGAGATCACAAAGTGTCTTATTTCCGGTTACTGGCGTTTATGCCCTGACTGAACTaa   >  1:4854506/1‑140 (MQ=255)

                                                                                                                                      aTGTAAAGGATCCATAGTGATTTCATCCATAAATAAGTGAACTAACTGCATCATATTTCTACCAAAAATAATCGGGTGCGAGAGAGATCACAAAGTGTCTTATTTCCGGTTACTGGCGTTTATGCCCTGACTGAACTAat  <  1:8685761/140‑1 (MQ=255)

CCCAGCGCCACGCCCAGCCCGGTCAGCACCCCTGCGCGTCGACCGGAAGCCAGGCTGGTTTGTACTACCACAAAGAGATTGGCTCCCGGATTAAAAAAAGTAATCACGAACAGTCCTACGGTCAGGTAAACGGCATGCAAAGGATCCATAGTGATTTCATCCATAAATAAGTGAACTAACTGCATCATATTTCTACCAAAAATAATCGGGTGCGAGAGAGATCACAAAGTGTCTTATTTCCGGTTACTGGCGTTTATGCCCTGACTGAACTAAT  >  NZ_CP025268/345201‑345474

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`