Predicted mutation
evidence seq id position mutation annotation gene description
RA NZ_CP025268 3,479,488 T→C *602* (TAA→TAG)  kefB ← glutathione‑regulated potassium‑efflux system protein KefB

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0252683,479,4880TC100.0% 1754.8 / NA 500*602* (TAA→TAG) kefBglutathione‑regulated potassium‑efflux system protein KefB
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (273/227);  total (273/227)

Only 100 of 501 total aligned reads displayed.

GCATCTGATGTCCGCACTTAACACATTCAACAATATCAATATTATTTTCGCGCCACATCGCCATTGAATCCTGCGCCTGACAGGCCGGGCATTTTGCGCCCGCAATAAAACGTTTTCGGATTGCCATCTTTACCCTTTACTCAAATTCATCCCAGCCGTCCAGCTGGCGTCGTTCTTGTTGCATTTCACGCTGGAAAATCTCTTCCAGTTCGCGTCGGGCTTCCCTGGCGCGAGAAATTTGTACGGTATCGGCATGCATTGGGATGAGCTCT  >  NZ_CP025268/3479352‑3479623
                                                                                                                                        |                                                                                                                                       
gCATCTGATGTCCGCACTTAACACATTCAACAATATCAATATTATTTTCGCGCCACATCGCCATTGAATCCTGCGCCTGACAGGCCGGGCATTTTGCGCCCGCACTAAAACGTTTTCGGATTGCCATCTTTACCCTCTAc                                                                                                                                      >  1:3821588/1‑140 (MQ=255)
 cATCTGATGTCCGCACTTAACACATTCAACAATATCAATATTATTTTCGCGCCACATCGCCATTGAATCCTGCGCCTGACAGGCCGGGCATTTTGCGCCCGCAATAAAACGTTTTCGGATTGCCATCTTTACCCTCTACt                                                                                                                                     <  1:2990304/140‑1 (MQ=255)
     tGATGTCCGCACTTAACACATTCAACAATATCAATATTATTTTCGCGCCACATCGCCATTGAATCCTGCGCCTGACAGGCCGGGCATTTTGCGCCCGCAATAAAACGTTTTCGGATTGCCATCTTTACCCTCTACTCaaa                                                                                                                                 >  1:1128329/1‑140 (MQ=255)
     tGATGTCCGCACTTAACACATTCAACAATATCAATATTATTTTCGCGCCACATCGCCATTGAATCCTGCGCCTGACAGGCCGGGCATTTTGCGCCCGCAATAAAACGTTTTCGGATTGCCATCTTTACCCTCTACTCaaa                                                                                                                                 >  2:710946/1‑140 (MQ=255)
       aTGTCCGCACTTAACACATTCAACAATATCAATATTATTTTCGCGCCACATCGCCATTGAATCCTGCGCCTGACAGGCCGGGCATTTTGCGCCCGCAATAAAACGTTTTCGGATTGCCATCTTTACCCTCTACTCAAAtt                                                                                                                               <  1:4617177/140‑1 (MQ=255)
        tGTCCGCACTTAACACATTCAACAATATCAATATTATTTTCGCGCCACATCGCCATTGAATCCTGCGCCTGACAGGCCGGGCATTTTGCGCCCGCAATAAAACGTTTTCGGATTGCCATCTATACCCTCTACTCAAATTc                                                                                                                              >  1:8430618/1‑140 (MQ=255)
            cGCACTTAACACATTCAACAATATCAATATTATTTTCGCTCCACATCTCCATTGAATCCTGCGCCTGACAGGCCGGGCATTTTGCGCCCGCAATAAAACGTTTTCGGATTGCCATCTTTACACTCTACTCAAATTCATcc                                                                                                                          >  1:7416723/1‑140 (MQ=255)
            cGCACTTAACACATTCAACAATATCAATATTATTTTCGCGCCACATCGCCATTGAATCCTGCGCCTGACAGGCCGGGCATTTTGCGCCCGCAATAAAACGTTTTCGGATTGCCATCTTTACCCTCTACTCAAATTCATcc                                                                                                                          >  2:1997408/1‑140 (MQ=255)
            cGCACTTAACACATTCAACAATATCAATATTATTTTCGCGCCACATCGCCATTGAATCCTGCGCCTGACAGGCCGGGCATTTTGCGCCCGCAATAAAACGTTTTCGGATTGCCATCTTTACCCTCTACTCAAATTCATcc                                                                                                                          <  2:1784602/140‑1 (MQ=255)
            cGCACTTAACACATTCAACAATATCAATATTATTTTCGCGCCACATCGCCATTGAATCCTGCGCCTGACAGGCCGGGCATTTTGCGCCCGCAATAAAACGTTTTCGGATTGCCATCTTTACCCTCTACTCAAATTCATcc                                                                                                                          >  1:7323635/1‑140 (MQ=255)
            cGCACTTAACACATTCAACAATATCAATATTATTTTCGCGCCACATCGCCATTGAATCCTGCGCCTGACAGGCCGGGCATTTTGCGCCCGCAATAAAACGTTTTCGGATTGCCATCTTTACCCTCTACTCAAATTCATcc                                                                                                                          >  1:3487145/1‑140 (MQ=255)
             gCACTTAACACATTCAACAATATCAATATTATTTTCGCGCCACATCGCCATTGAATCCTGCGCCTGACAGGCCGGGCATTTTGCGCCCGCAATAAAACGTTTTCGGATTGCCATCTTTACCCTCTACTCAAATTCATccc                                                                                                                         <  1:6892164/140‑1 (MQ=255)
              cACTTAACACATTCAACAATATCAATATTATTTTCGCGCCACATCGCCATTGAATCCTGCGCCTGACAGGCCGGGCATTTTGCGCCCGCAATAAAACGTTTTCGGATTGCCATCTTTACCCTCTCCTCAAATTCATCCCa                                                                                                                        <  2:7851139/140‑1 (MQ=255)
               aCTTAACACATTCAACAATATCAATATTATTTTCGCGCCACATCGCCATTGAATCCTGCGCCTGACAGGCCGGGCATTTTGCGCCCGCAATAAAACGTTTTCGGATTGCCATCTTTACCCTCTACTCAAATTCATCCCAg                                                                                                                       <  2:5733094/140‑1 (MQ=255)
                  tAACACATTCAACAATATCAATATTATTTTCGCGCCACATCGCCATTGAATCCTGCGCCTGACAGGCCGGGCATTTTGCGCCCGCAATAAAACGTTTTCGGATGGCCATCTTTACCCTCTACTCAAATTCATCCCAGCCg                                                                                                                    <  2:1074655/140‑1 (MQ=255)
                   aaCACATTCAACAATATCAATATTATTTTCGCGCCACATCGCCATTGAATCCTGCGCCTGACAGGCCGGGCATTTTGCGCCCGCAATAAAACGTTTTCGGATTGCCATCTTTACCCTCTACTCAAATTCATCCCAGCCGt                                                                                                                   >  2:2730958/1‑140 (MQ=255)
                   aaCACATTCAACAATATCAATATTATTTTCGCGCCACATCGCCATTGAATCCTGCGCCTGACAGGCCGGGCATTTTGCGCCCGCAATAAAACGTTTTCGGATTGCCATCTTTACCCTCTACTCAAATTCATCCCAGCCGt                                                                                                                   >  1:6773956/1‑140 (MQ=255)
                        aTTCAACAATATCAATATTATTTTCGCGCCACATCGCCATTGAATCCTGCGCCTGACAGGCCGGGCATTTTGCGCCCGCAATAAAACGTTTTCGGATTGCCATCTTTACCCTCTACTCAAATTCATCCCAGCCGTCCAGc                                                                                                              <  2:1624236/140‑1 (MQ=255)
                         ttCAACAATATCAATATTATTTTCGCGCCACATCGCCATTGAATCCTGCGCCTGACAGGCCGGGCATTTTGCGCCCGCAATAAAACGTTTTCGGATTGCCATCTTTACCCTCTACTCAAATTCATCCCAGCCGTCCAGCt                                                                                                             >  2:2608355/1‑140 (MQ=255)
                         ttCAACAATATCAATATTATTTTCGCGCCACATCGCCATTGAATCCTGCGCCTGACAGGCCGGGCATTTTGCGCCCGCAATAAAACGTTTTCGGATTGCCATCTTTACCCTCTACTCAAATTCATCCCAGCCGTCCAGCt                                                                                                             >  2:4888367/1‑140 (MQ=255)
                         ttCAACAATATCAATATTATTTTCGCGCCACATCGCCATTGAATCCTGCGCCTGACAGGCCGGGCATTTTGCGCCCGCAATAAAACGTTTTCGGATTGCCATCTTTACCCTCTACTCAAATTCATCCCAGCCGTCCAGCt                                                                                                             >  1:5466183/1‑140 (MQ=255)
                         ttCAACAATATCAATATTATTTTCGCGCCACATCGCCATTGAATCCTGCGCCTGACAGGCCGGGCAATTTGCGCCCGCAATAAAACGTTTTCGGATTGCCATCTTTACCCTCTACTCAAATTCATCACAGCCGTCCAGCt                                                                                                             >  2:4480503/1‑140 (MQ=255)
                          tCAACAATATCAATATTATTTTCGCGCCACATCGCCATTGAATCCTGCGCCTGACAGGCCGGGCATTTTGCGCCCGCAATAAAACGTTTTCGGATTGCCATCTTTACCCTCTACTCAAATTCATCCCAGCCGTCCAGCTg                                                                                                            >  1:5799411/1‑140 (MQ=255)
                           caacaaTATCAATATTATTTTCGCGCCACATCGCCATTGAATCCTGCGCCTGACAGGCCGGGCATTTTGCGCCCGCAATAAAACGTTTTCGTATTGAAATCTGTACCCTCTACTCAAATTCATCCCAGCCGTCCAGCTgg                                                                                                           >  2:9346544/1‑140 (MQ=255)
                           caacaaTATCAATATTATTTTCGCGCCACATCGCCATTGAATCCTGCGCCTGACAGGCCGGGCATTTTGCGCCCGCAATAAAACGTTTTCGGATTGCCATCTTTACCCTCTACTCAAATTCATCCCAGCCGTCCAGCTgg                                                                                                           >  2:6212289/1‑140 (MQ=255)
                           caacaaTATCAATATTATTTTCGCGCCACATCGCCATTGAATCCTGCGCCTGACAGGCCGGGCATTTTGCGCCCGCAATAAAACGTTTTCGGATTGCCATCTTTACCCTCTACTCAAATTCAACCCAGCCGTCCAGCTgg                                                                                                           >  2:2034104/1‑140 (MQ=255)
                            aacaaTATCAATATTATTTTCGCGCCACATCGCCATTGAATCCTGCGCCTGACAGGCCGGGCATTTTGCGCCCGCAATAAAACGTTTTCGGATTGCCATCTTTACCCTCTACTCAAATTCATCCCAGCCGTCCAGCTGGc                                                                                                          <  1:1004241/140‑1 (MQ=255)
                             acaaTATCAATATTATTTTCGCGCCACATCGCCATTGAATCCTGCGCCTGACAGGCCGGGCATTTTGCGCCCGCAATAAAACGGTTTCGGATTGCCATCTTTACCCTCTACTCAAATTCATCCCAGCCGTCCAGCTGGcg                                                                                                         >  1:895830/1‑140 (MQ=255)
                              caatatcaatatTATTTTCGCGCCACATCGCCATTGAATCCTGCGCCTGACAGGCCGGGCATTTTGCGCCCGCAATAAAACGTTTTCGGATTGCCATCTTTACCCTCTACTCAAATTCATCCCAGCCGTCCAGCTGGcgt                                                                                                        <  1:9407456/140‑1 (MQ=255)
                                 tatcaatatTATTTTCGCGCCACATCGCCATTGAATCCTGCGCCTGACAGGCCGGGCATTTTGCGCCCGCAATAAAACGTTTTCGGATTGCCATCTTTACCCTCTACTCACATTCATCCCAGCCGTCCAGCTGGcgtcgt                                                                                                     >  2:6718775/1‑140 (MQ=255)
                                 tatcaatatTATTTTCGCGCCACATCGCCATTGAATCCTGCGCCTGACAGGCCGGGCATTTTGCGCCCGCAATAAAACGTTTTCGGATTGCCATCTTTACCCTCTACTCAAATTCATCCCAGCCGTCCAGCTGGcgtcgt                                                                                                     >  1:1767919/1‑140 (MQ=255)
                                  atcaatatTATTTTCGCGCCACATCGCCATTGAATCCTGCGCCTGACAGGCCGGGCATTTTGCGCCCGCAATAAAACGTTTTCGGATTGCCATCTTTACCCTCTACTCAAATTCATCCCAGCCGTCCAGCTGGCGTCGtt                                                                                                    >  1:2041218/1‑140 (MQ=255)
                                  atcaatatTATTTTCGCGCCACATCGCCATTGAATCCTGCGCCTGACAGGCCGGGCATTTTGCGCCCGCAATAAAACGTTTTCGGATTGCCATCTTTACCCTCTACTCAAATTCATCCCAGCCGTCCAGCTGGCGTCGtt                                                                                                    >  2:6040717/1‑140 (MQ=255)
                                   tcaatatTATTTTCGCGCCACATCGCCATTGAATCCTGCGCCTGACAGGCCGGGCATTTTGCGCCCGCACTAAAACGTTTTCGGATTGCCATCTTTACCCTCTACTCAAATTCATCCCAGCCGTCCAGCTGGCGTCGTTc                                                                                                   >  1:3356571/1‑140 (MQ=255)
                                   tcaatatTATTTTCGCGCCACATCGCCATTGAATCCTGCGCCTGACAGGCCGGGCATTTTGCGCCCGCAATAAAACGTTTTCGGATTGCCATCTTTACCCTCTACTCAAATTCATCCCAGCCGTCCAGCTGGCGTCGTTc                                                                                                   <  1:8686629/140‑1 (MQ=255)
                                    caatatTATTTTCGCGCCACATCGCCATTGAATCCTGCGCCTGACAGGCCGGGCATTTTGCGCCCGCACTAAAACGTTTTCGGATTGCCATCTTTACCCTCTACTCAAATTCATCCCAGCCGTCCAGCTGGCGTCGTTCt                                                                                                  >  2:4991547/1‑140 (MQ=255)
                                     aatatTATTTTCGCGCCACATCGCCATTGAATCCTGCGCCTGACAGGCCGGGCATTTTGCGCCCGCAATAAAACGTTTTCGGATTGCCATCTTTACCCTCTACTCAAATTCATCCCAGCCGTCCAGCTGGCGTCGTTCtt                                                                                                 >  1:7990938/1‑140 (MQ=255)
                                      atattatattCGCGCCACATCGCCATTGAATCCTGCGCCTGACAGGCCGGGCATTATGCGCCCGCAATAAAACGTATTCGGATTGACATCTTTACCCTCTACACAAATACATCCCAGCCGTCCAGCTGGCCTCGTTCtgg                                                                                                >  2:7990488/1‑138 (MQ=255)
                                      atatTATTTTCGCGCCACATCGCCATTGAATCCTGCGCCTGACAGGCCGGGCATTTTGCGCCCGCAATAAAACGTTTTCGGATTGCCATCTTTACCCTCTACTCAAATTCATCCCAGCCGTCCAGCTGGCGTCGTTCttg                                                                                                >  1:6348549/1‑140 (MQ=255)
                                      atatTATTTTCGCGCCACATCGCCATTGAATCCTGCGCCTGACAGGCCGGGCATTTTGCGCCCGCAATAAAACGTTTTCGGATTGCCATCTTTACCCTCTACTCAAATTCATCCCAGCCGTCCAGCTGGCGTCGTTCttg                                                                                                >  1:3420092/1‑140 (MQ=255)
                                         ttattTTCGCGCCACATCGCCATTGAATCCTGCGCCTGACAGGCCGGGCATTTTGCGCCCGCAATAAAACGTTTTCGGATTGCCATCTTTACCCTCTACTCAAATTCATCCCAGCCGTCCAGCTGGCGTCGTTCttgttg                                                                                             <  2:2453380/140‑1 (MQ=255)
                                           attTTCGCGCCACATCGCCATTGAATCCTGCGCCTGACAGGCCGGGCATTTTGCGCCCGCAATAAAACGTTTTCGGATTGCCATCTTTACCCTCTACTCAAATTCATCCCAGCCGTCCAGCTGGCGTCGTTCTTGTTGCa                                                                                           >  1:1693680/1‑140 (MQ=255)
                                           attTTCGCGCCACATCGCCATTGAATCCTGCGCCTGACAGGCCGGGCATTTTGCGCCCGCAATAAAACGTTTTCGGATTGCCATCTTTACCCTCTACTCAAATTCATCACAGCCGTCCAGCTGGCGTCGTTCTTGTTGCa                                                                                           >  2:3104982/1‑140 (MQ=255)
                                            ttttCGCGCCACATCGCCATTGAATCCTGCGCCTGACAGGCCGGGCATTTTGCGCCCGCAATAAAACGTTTTCGGATAGCCATCTTTACCCTCTACTCAAATTCATCCCAGCCGTCCAGCTGGCGTCGTTCTTGTTGAAt                                                                                          >  2:8457371/1‑140 (MQ=255)
                                               tCGCGCCACATCGCCATTGAATCCTGCGCCTGACAGGCCGGGCATTTTGCGCCCGCAATAAAACGTTTTCGGATTGCCATCTTTTCCCTCTACTCAAATTCATCCCAGCCGTCCAGCTGGCGTCGTTCTTGTTGCAGTTc                                                                                       >  2:5596426/1‑140 (MQ=255)
                                               tCGCGCCACATCGCCATTGAATCCTGCGCCTGACAGGCCGGGCATTTTGCGCCCGCAATAAAACGTTTTCGGATTGCCATCTTTACCCTCTACTCAAATTCATCCCAGCCGTCNAGCTGGCGTCGTTCTTGTTGCATTTc                                                                                       >  1:7107901/1‑140 (MQ=255)
                                               tCGCGCCACATCGCCATTGAATCCTGCGCCTGACAGGCCGGGCATTTTGCGCCCGCAATAAAACGTTTTCGGATTGCCATCTTTACCCTCTACTCAAATTCATCCCAGCCGTCCAGCTGGCGTCGTTCTTGTTGCATTTc                                                                                       >  2:3712182/1‑140 (MQ=255)
                                               tCGCGCCACATCGCCATTGAATCCTGCGCCTGACAGGCCGGGCATTGTGCGCCCGCAATCAAACGTTTTCGGATTGCCTGCTTGACCCTCTACTCAAATTCATCCCAGCCGGCCAGCTGGCGTCGTGCTTGTTGCATGTc                                                                                       >  2:8597771/1‑140 (MQ=255)
                                                 gcgcCACATCGCCATTGAATCCTGCGCCTGACAGGCCGGGCATTTTGCGCCCGCAATAAAACGTTTTCGGATTGCCATCTTTACCCTCTACTCAAATTCATCCCAGCCGTCCAGCTGGCGTCGTTCTTGTTGCATTTCAc                                                                                     >  1:6501860/1‑140 (MQ=255)
                                                 gcgcCACATCGCCATTGAATCCTGCGCCTGACAGGCCGGGCATTTTGCGCCCGCAATAAAACGTTTTCGGATTGCCATCTTTACCCTCTACTCAAATTCATCCCAGCCGTCCAGCTGGCGTCGTTCTTGTTGCATTTCAc                                                                                     >  1:1843952/1‑140 (MQ=255)
                                                 gcgcCACATCGCCATTGAATCCTGCGCCTGACAGGCCGGGCATTTTGCGCCCGCAATAAAACGTTTTCGGATTGCCATCTTTACCCTCTACTCAAATTCATCCCAGCCGTCCAGCTGGCGTCGTTCTTGTTGCATTTCAc                                                                                     >  1:6070476/1‑140 (MQ=255)
                                                 gcgcCACATCGCCATTGAATCCTGCGCCTGACAGGCCGGGCATTTTGCGCCCGCAATAAAACGTTTTCGGATTGCCATCTTTACCCTCTACTCAAATTCATCCCAGCCGTCCAGCTGGCGTCGGGCTTGTTGCATTTCAc                                                                                     >  1:2381911/1‑140 (MQ=255)
                                                  cgcCACATCGCCATTGAATCCTGCGCCTGACAGGCCGGGCATTTTGCGCCCGCAATAAAACGTTTTCGGATTGCCATCTTTACCCTCTACTCAAATTCATCCCAGCCGTCCAGCTGGCGTCGTTCTTGTTGCATTTCACg                                                                                    <  1:5682530/140‑1 (MQ=255)
                                                  cgcCACATCGCCATTGAATCCTGCGCCTGACAGGCCGGGCATTTTGCGCCCGCAATAAAACGTTGTCGGATTGCCATCTTTACCCTCTACTCAAAGTCATCCCAGCCGTCCAGCTGGCGTCGTTCTTGTTGCATTTCACg                                                                                    <  2:2354980/140‑1 (MQ=255)
                                                         tCGCCATTGAATCCTGCGCCTGACAGGCCGGGCATTTTGCGCCCGCAATAAAACGTTTTCGGATTGCCATCTTTACCCTCTACTCAAATTCATCCCAGCCGTCCAGCTGGCGTCGTTCTTGTTGCATTTCACGCTGGaaa                                                                             <  1:5575443/140‑1 (MQ=255)
                                                           gCCATTGAATCCTGCGCCTGACAGGCCGTGCATTTTGCGCACGCAATAAAACGTTTTCGGATTGCCATCTTTACCCTCTACTCAAATTCATCCCAGCCGTCCAGCTGGCGTCGTTCTTGTTGCATTTCACGCTGGAAAAt                                                                           <  2:6958687/140‑1 (MQ=255)
                                                                 gAATCCTGCGCCTGACAGGCCGGGCATTTTGCGCCCGCAATAAAACGTTTTCGGATTGCCATCTTTACCCTCTACTCAAATTCATCCCAGCCGTCCAGCTGGCGTCGTTCTTGTTGCATTTCACGCTGGAAAATCTCTTc                                                                     <  2:533970/140‑1 (MQ=255)
                                                                    tCCTGCGCCTGACAGGCCGGGCATTTTGCGCCCGCCATAAAACGTTTTCGGATTGCCATCTGTACCCTCTACTCAAATTCATCCCAGCCGTCCAGCTGGCGTCGTGCTGGTTGCATTTCACGCTGGAAAATCTCTTCCAg                                                                  >  1:134225/1‑140 (MQ=255)
                                                                    tCCTGCGCCTGACAGGCCGGGCATTTTGCGCCCGCAATAAAACGTTTTCGGATTGCCATCTTTACCCTCTACTCAAATTCATCCCAGCCGTCCAGCTGGCGTCGTTCTTGTTGCATTTCACGCTGGAAAATCTCTTCCAg                                                                  >  2:8145400/1‑140 (MQ=255)
                                                                    tCCTGCGCCTGACAGGCCGGGCATTTTGCGCCCGCAATAAAACGTTTTCGGATTGCCATCTTTACCCTCTACTCAAATTCATCCCAGCCGTCCAGCTGGCGTCGTTCTTGTTGCATTTCACGCTGGAAAATCTCTTCCAg                                                                  >  1:7005243/1‑140 (MQ=255)
                                                                     ccTGCGCCCGACAGGCCGGGCATTTTGCGCCCGCAATAAAACGTTTTCGGATTGCCATCTTTACCCTCTACTCAAATTCATCCCAGCCGTCCAGCTGGCGTCGTTCTTGTTGCATTTCACGCTGGAAAATCTCTTCCAGt                                                                 <  1:9222776/140‑1 (MQ=255)
                                                                       tGCGCCTGACAGGCCGGGCATTTTGCGCCCGCAATAAAACGTTTTCGGATTGCCATCTTTACCCTCTACTCAAATTCATCCCAGCCGTCCAGCTGGCGTCGTTCTTGTTGCATTTCACGCTGGAAAATCTCTTCCAGTTc                                                               >  2:8738515/1‑140 (MQ=255)
                                                                       tGCGCCTGACAGGCCGGGCATTTTGCGCCCGCAATAAAACGTTTTCGGATTGCCATCTTTACCCTCTACTCAAATTCATCCCAGCCGTCCAGCTGGCGTCGTTCTTGTTGCATTTCACGCTGGAAAATCTCTTCCAGTTc                                                               >  1:8274442/1‑140 (MQ=255)
                                                                          gcCTGACAGGCCGGGCATTTTGCGCCCGCAATAAAACGTTTTCGGATTGCCATCTTTACCCTCTACTCAAATTCATCCCAGCCGTCCAGCTGGCGTCGTTCTTGTTGCATTTCACGCTGGAAAATCTCTTCCAGTTcgcg                                                            >  2:3901827/1‑140 (MQ=255)
                                                                            cTGACAGGCCGGGCATTTTGCGCCCGCAATAAAACGTTTTCGGATTGCCATCTTTACCCTCTACTCAAATTCATCCCAGCCGTCCAGCTGGCGTCGTTCTTGTTGCATTTCACGCTGGAAAATCTCTTCCAGTTCGCGTc                                                          >  1:4120358/1‑140 (MQ=255)
                                                                             tGACAGGCCGGGCATTTTGCGCCCGCAATAAAACGTTTTCGGATTGCCATCTTTACCCTCTACTCAAATTCATCCCAGCCGTCCAGCTGGCGTCGTTCTTGTTGCATTTCACGCTGGAAAATCTCTTCCAGTTCGCGTCg                                                         <  2:4604883/140‑1 (MQ=255)
                                                                               aCAGGCCGGGCATTTTGCGCCCGCAATAAAACGTGTTCGGATTGCCATCTTTACCCTCTACTCAAATTCATCCCAGCCGTCCAGCTGGCGTCGTTCTTGTTGCATTTCACGCTGGAAAATCTCTTCCAGTTCGCGTCggg                                                       <  2:7709166/140‑1 (MQ=255)
                                                                                   gCCGGGCATTTTGCGCCCGCAATAAAACGTTTTCGGATTGCCATCTTTAACCTCTACTCAAATTCATCCCAGCCGTCCAGCTGGCGACGTTCTTGTTGCATTTCACGCTGGAAAATCTCTTCCAGTTCGCGTCGGGCTTc                                                   >  2:889174/1‑140 (MQ=255)
                                                                                        gCATTTTGCGCCCGCAATAAAACGTTTTCGGATTGCCATCTTTACCCTCTACTCTAAGTCATCCCAGCCGTCCAGCTGGCGTCGTTCTTGTTGCATTTAACGCTGGAATATCTCTTCCAGTTCGCGTCGGGCTTcggtgg                                              >  2:9178047/1‑135 (MQ=255)
                                                                                        gCATTTTGCGCCCGCAATAAAACGTTTTCGGATTGCCATCTTTACCCTCTACTCAAATTCATCCCAGCCGTCCAGCTGGCGTCGTTCTTGTTGCATTTCACGCTGGAAAATCTCTTCCAGTTCGCGTCGGGCTTCCCTgg                                              <  1:7502694/140‑1 (MQ=255)
                                                                                             ttGCGCCCGCAATAAAACGTTTTCGGATTGCCATCTTTACCCTCTACACAAATTCATCCCAGCCGTCCAGCTGGCGTCGTTCTTGTTGCATTTCACGCTGGAAAATCTCTTCCAGTTCGCGTCGGGCTTCCCTGGCGCga                                         <  1:3211746/140‑1 (MQ=255)
                                                                                                cgcCCGCAATAAAACGTTTTCGGATTGCCATCTTTACCCTCTACTCAAATTCATCCCAGCCGTCCAGCTGGCGTCGTTCTTGTTGCATTTCACGCTGGAAAATCTCTTCCAGTTCGCGTCGGGCTTCCCTGGCGCGAGaa                                      <  2:3365403/140‑1 (MQ=255)
                                                                                                  cccGCAATAAAACGTTTTCGGATTGCCATCTTTACCCTCTACTCAAATTCATCCCAGCCGTCCAGCTGGCGTCGTTCTTGTTGCATTTCACGCTGGAAAATCTCTTCCAGTTCGCGTCGGGCTTCCCTGGCGCGAGAAAt                                    <  2:1242723/140‑1 (MQ=255)
                                                                                                  cccGCAATAAAACGTTTTCGGATTGCCATCTTTACCCTCTACTCAAATTCATCCCAGCCGTCCAGCTGGCGTCGTTCTTGTTGCATTTCACGCTGGAAAATCTCTTCCAGTTCGCGTCGGGCTTCCCTGGCGCGAGAAAt                                    <  1:6725274/140‑1 (MQ=255)
                                                                                                  cccGCAATAAAACGTTTTCGGATTGCCATCTTTACCCTCTACTCAAATTCATCCCAGCCGTCCAGCTGGCGTCGTTCTTGTTGCATTTCACGCTGGAAAATCTCTTCCAGTTCGCGTCGGGCTTCCCTGGCGCGAGAAAt                                    >  2:6450585/1‑140 (MQ=255)
                                                                                                   ccGCAATAAAACGTTTTCGGATTGCCATCTTTACCCTCTACTCAAATTCATCCCAGCCGTCCAGCTGGCGTCGTTCTTGTTGCATTTCACGCTGGAAAATCTCTTCCAGTTCGCGTCGGGCTTCCCTGGCGCGAGAAAtt                                   <  1:5260620/140‑1 (MQ=255)
                                                                                                       aaTAAACCGTTTTCGGAGTGCCATCTTTACCCTCTCCTCAAATTCATCCCAGCCGTCCAGCTGGCGTCGTTCTTGTTGCATTTCACGCTGGAAAATCTCTTCCAGTTCGCGTCGGGCTTCCCTGGCGCGAGAAATTTGTa                               <  2:6776518/140‑1 (MQ=255)
                                                                                                       aaTAAAACGTTTTCGGATTGCCATCTTTACCCTCTACTCAAATTCATCCCAGCCGTCCAGCTGGCGTCGTTCTTGTTGCATTTCACGCTGGAAAATCTCTTCCAGTTCGCGTCGGGCTTCCCTGGCGCGAGAAATTTGTa                               <  1:2650156/140‑1 (MQ=255)
                                                                                                       aaTAAAACGTTTTCGGATTGCCATCTTTACCCTCTACTCAAATTCATCCCAGCCGTCCAGCTGGCGTCGTTCTTGTTGCATTTCACGCTGGAAAATCTCTTCCAGTTCGCGTCGGGCTTCCCTGGCGCGAGAAATTTGTa                               <  2:6357311/140‑1 (MQ=255)
                                                                                                       aaTAAAACGTTTTCGGATTGCCATCTTTACCCTCTACTCAAATTCATCCCAGCCGTCCAGCTGGCGTCGTTCTTGTTGCATTTCACGCTGGAAAATCTCTTCCAGTTCGCGTCGGGCTTCCCTGGCGCGAGAAATTTGTa                               <  1:1457733/140‑1 (MQ=255)
                                                                                                       aaTAAAACGTTTTCGGATTGCCATCTTTACCCTCTACTCAAATTCATCCCAGCCGTCCAGCTGGCGTCGTTCTTGTTGCATTTCACGCTGGAAAATCTCTTCCAGTTCGCGTCGGGCTTCCCTGGCGCGAGAAATTTGTa                               <  2:1929182/140‑1 (MQ=255)
                                                                                                       aaTAAAACGTTTTCGGATTGCCATCTTTACCCTCTACTCAAATTCATCCCAGCCGTCCAGCTGGCGTCGTTCTTGTTGCATTTCACGCTGGAAAATCTCTTCCAGTTCGCGTCGGGCTTCCCTGGCGCGAGAAATTTGTa                               <  1:8217356/140‑1 (MQ=255)
                                                                                                                 tttCGGATTGCCCTCTTTACCCTCTACTCAAATTCATCCCAGCCGTCCAGCTGGCGTGGTTCTTGTTGCATTTGACGCTGGACACTCTCGTCCAGTTCGCGTCGGGCTTCCCTGGCGCGAGAAAGTGGTGCGGTATCGgc                     >  2:1435855/1‑140 (MQ=255)
                                                                                                                 tttCGGATTGCCATCTTTACCCTCTACTCAAATTCATCCCAGCCGTCCAGCTGGCGTCGTTCTTGTTGCATTTCACGCTGGAAAATCTCTTCCAGTTCGCGTCGGGCTTCCCTGGCGCGAGAAATTTGTACGGTATCGgc                     <  1:9127850/140‑1 (MQ=255)
                                                                                                                 tttCGGATTGCCATCTTTACCCTCTACTCAAATTCATCCCAGCCGTCCAGCTGGCGTCGTTCTTGTTGCATTTCACGCTGGAAAATCTCTTCAAGTTCGCGTCGGGCTTCCCTGGCGCGAGAACTTTGTACGGTATCGgc                     >  2:7454239/1‑140 (MQ=255)
                                                                                                                 tttCGGATTGCCATCTTTACCCTCTACTCAAAGTCATCCCAGCCGTCCAGCTGGCGTCGTTCTTGTTGCATTTCACGCTGGAAAATCTCTTCCAGTTCGCGTCGGGCTTCCCTGGCGCGAGAAATTTGTACGGTATCGgc                     <  2:5098780/140‑1 (MQ=255)
                                                                                                                 tgtCGGATTGCCATCTTTACCCTCTACTCAAATTCCTCCCAGCCGTCCAGCTGGCGTCGTTCTTGTTGCATTTCACGCTGGATAATCTCTTCCAGTTCGCTTCGGGCTTCCCTGTCGCGAGAAATTTGTACGGTATCTgc                     <  2:9061840/138‑1 (MQ=255)
                                                                                                                 tgtCGGATTGCCATCTTTACCCTCTACTCAAATTCATCCCAGCCGTCCAGCTGGCGTCGTTCTTGTTGCATTTCACGCTGGAAAATCTCTTCCAGTTCGCGTCGGGCTTCCCTGGCGCGAGAAATTTGTACGGTATCGgc                     <  2:8058534/138‑1 (MQ=255)
                                                                                                                  ttCGGATTGCCATCTTTACCCTCTACTCAAATTCATCCCAGCCGTCCAGCTGGCGTCGTTCTTGTTGCATTTCACGCTGGAAAATCTCTTCCAGTTCGCGTCGGGCTTCCCTGGCGCGAGAAATTTGTACGGTATCGgca                    <  1:5325547/140‑1 (MQ=255)
                                                                                                                  ttCGGATTGCCATCTTTACCCTCTACTCAAATTCATCCCAGCCGTCCAGCTGGCGTCGTTCTTGTTGCATTTCACGCTGGAAAATCTCTTCCAGTTCGCGTCGGGCTTCCCTGGCGCGAGAAATTTGTACGGTATCGgca                    <  2:1348275/140‑1 (MQ=255)
                                                                                                                  ttCGGATTGCCATCTTTACCCTCTACTCAAATTCATCCCAGCCGTCCAGCTGGCGTCGTTCTTGTTGCATTTCACGCTGGAAAATCTCTTCCAGTTCGCGTCGGGCTTCCCTGGCGCGAGAAATTTGTACGGTATCGgca                    <  1:640725/140‑1 (MQ=255)
                                                                                                                   tCGGATTGCCATCTTTACCCTCTACTCAAATTCATCCCAGCCGTCCAGCTGGCGTCGTTCTTGTTGCATTTCACGCTGGAAAATCTCTTCCAGTTCGCGTCGGGCTTCCCTGGCGCGAGAAATTTGTACGGTATCGgcat                   >  1:1168747/1‑140 (MQ=255)
                                                                                                                      gATTGCCATCTTTACCCTCTACTCAAATTCATCCCAGCCGTCCAGCTGGCGTCGTTCTTGTTGCATTTCACGCTGGAAAATCTCTTCCAGTTCGCGTCGGGCTTCCCTGGCGCGAGAAATTTGTACGGTATCGgcatgca                <  1:5044981/140‑1 (MQ=255)
                                                                                                                       aTTGCCATCTTTACCCTCTACTCAAATTCATCCCAGCCGTCCAGCTGGCGTCGTTCTTGTTGCATTTCACGCTGGAAAATCTCTTCCAGTTCGCGTCGGGCTTCCCTGGCGCGAGAAATTTGTACGGTATCGgcatgcat               <  1:4755693/140‑1 (MQ=255)
                                                                                                                       aTTGCCATCTTTACCCTCTACTCAAATTCATCCCAGCCGTCCAGCTGGCGTCGTTCTTGTTGCATTTCACGCTGGAAAATCTCTTCCAGTTCGCGTCGGGCTTCCCTGGCGCGAGAAATTTGTACGGTATCGgcatgcat               <  2:4229464/140‑1 (MQ=255)
                                                                                                                         tGCCATCTTTACCCTCTACTCAAATTCATCCCAGCCGTCCAGCTGGCGTCGTGCTTGTTGCATTTCACGCTGGACAATCTCTTCCAGTTCGCGTCGGGCTTCCCTGGCGCGTGAAATTTGTACGGTATCGgcatgaagtg             >  2:8268950/1‑135 (MQ=255)
                                                                                                                           ccATCTTTACCCTCTACTCAAATTCATCCCAGCCGTCCAGCTGGCGTCGTTCTTGTTGCATTTCACGTTGGAAAATCTCtt                                                                      <  1:6237596/81‑1 (MQ=255)
                                                                                                                              tCTTTACCCTCTACTCAAATTCATCCCAGCCGTCCAGCTGGCGTCGTTCTTGTTGCATTTCACGCTGGAAAATCTCTTCCAGTTCGCGTCGGGCTTCCCTGGCGCGAGAAATTTGTACGGTATCGGCATGCATTGGGATg        <  2:7274669/140‑1 (MQ=255)
                                                                                                                                 ttACCCTCTACTCAAATTCATCCCAGCCGTCCAGCTGGCGTCGTTCTTGTTGCATTTCACGCTGGAAAATCTCTTCCAGTTCGCGTCGGGCTTCCCTGGCGCGAGAAATTTGTACGGTATCGGCATGCATTGGGATGAGc     >  1:3924029/1‑140 (MQ=255)
                                                                                                                                  tACCCTCTACTCAAATTCATCCCAGCCGTCCAGCTGGCGTCGTTCTTGTTGCATTTCACGCTGGAAAATCTCTTCCAGTTCGCGTCGGGCTTCCCTGGCGCGAGAAATTTGTACGGTATCGGCATGCATTGGGATGAGct    <  1:7837233/140‑1 (MQ=255)
                                                                                                                                        |                                                                                                                                       
GCATCTGATGTCCGCACTTAACACATTCAACAATATCAATATTATTTTCGCGCCACATCGCCATTGAATCCTGCGCCTGACAGGCCGGGCATTTTGCGCCCGCAATAAAACGTTTTCGGATTGCCATCTTTACCCTTTACTCAAATTCATCCCAGCCGTCCAGCTGGCGTCGTTCTTGTTGCATTTCACGCTGGAAAATCTCTTCCAGTTCGCGTCGGGCTTCCCTGGCGCGAGAAATTTGTACGGTATCGGCATGCATTGGGATGAGCTCT  >  NZ_CP025268/3479352‑3479623

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑