BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP025268	3,504,638	A→G	262 (TAA→TAG)	frlD →	fructoselysine 6‑kinase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP025268	3,504,638	0	A	G	99.5%	1389.4 / NA	399	262 (TAA→TAG)	frlD	fructoselysine 6‑kinase
Reads supporting (aligned to +/- strand): ref base A (0/0); major base G (170/227); minor base T (0/2); total (170/229)
Fisher's exact test for biased strand distribution p-value = 5.10e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 407 total aligned reads displayed.

ATCGACACCATGGGTGCCGGAGATTCGTTCATTGCCGGATTCCTTTGCGGCTGGTCTGCGGGGATGACATTACCGCAGGCGATAGCGCAGGGAACGGCGTGCGCGGCGAAAACCATTCAGTACCACGGTGCCTGGTAAGTATAACGTTGGCGTGAGCATCTTCACGCCAACGTGCTGTTACTTGCCGGAAAACGACCCTATAATCCGAGTAATTCATTCTTTATTTCAGGGTCGATTATGTCAGCTACGGACCGCTACTCTCATCAACTCCT  >  NZ_CP025268/3504501‑3504772

 tCGACACCATGGGTGCCGGAGATTCGTTCATTGCCGGATTCCTTTGCGGCTGGTCTGCGGGGATGACATTACCGCAGGCGATAGCGCAGGGAACGGCGTGCGCGGCGAAAACCATTCAGTACCACGGTGCCTggtaggta                                                                                                                                     <  1:8825660/140‑1 (MQ=255)

 tCGACACCATGGGTGCCGGAGATTCGTTCATTGCCGGATTCCTTTGCGGCTGGTCTGCGGGGATGACATTACCGCAGGCGATAGCGCAGGGAACGGCGTGCGCGGCGAAAACCATTCAGTACCACGGTGCCTggtaggta                                                                                                                                     <  1:6614778/140‑1 (MQ=255)

        cATGGGTGCCGGAGATTCGTTCATTGCCGGATTCCTTTGCGGCTGGTCTGCGGGGATGACATTACCGCAGGCGATAGCGCAGGGAACGGCGTGCGCGGCGAAAACCATTCAGTACCACGGTGCCTGGTAGGTATAACGtt                                                                                                                              >  1:5550621/1‑140 (MQ=255)

          tGGGTGCCGGAGATTCGTTCATTGCCGGATTCCTTTGCGGCTGGTCTGCGGGGATGACATTACCGCAGGCGATATCGCAGGGAACGGCGTGCGCGGAGAAAACCATTCAGTACCACGGTGCCTGGTAGGTATAACGTTgg                                                                                                                            >  1:943356/1‑140 (MQ=255)

          tGGGTGCCGGAGATTCGTTCATTGCCGGATTCCTTTGCGGCTGGTCTGCGGGGATGACATTACCGCAGGCGATAGCGCAGGGAACGGCGTGCGCGGCGAAAACCATTCAGTACCACGGTGCCTGGTAGGTATAACGTTgg                                                                                                                            >  1:7212829/1‑140 (MQ=255)

          tGGGTGCCGGAGATTCGTTCATTGCCGGATTCCTTTGCGGCTGGTCTGCGGGGATGACATTACCGCAGGCGATAGCGCAGGGAACGGCGTGCGCGGCGAAAACCATTCAGTACCACGGTGCCTGGTAGGTATAACGTTgg                                                                                                                            >  2:1053933/1‑140 (MQ=255)

          tGGGTGCCGGAGATTCGTTCATTGCCGGATTCCATTGCGGCTGGTCTGCGGGGATGACATTACCGCAGGCGAGAGCGCAGGGAACGGCGTGCGCGGCGAAATCCAGTCAGAACCACGGTGCCTGGTAGGTATAACGTTgg                                                                                                                            >  1:3798838/1‑140 (MQ=255)

           gggTGCCGGAGATTCGTTCATTGCCGGATTCCTTTGCGGCTGGTCTGCGGGGATGACATTACCGCAGGCGATAGCGCAGGGAACGGCGTGCGCGGCGAAAACCATTCAGTACCACGGTGCCTGGTAGGTATAACGTTGGc                                                                                                                           >  1:9231329/1‑140 (MQ=255)

           gggTGCCGGAGATTCGTTCATTGCCGGATTCCTTTGCGGCTGGTCTGCGGGGATGACATTACCGCAGGCGATAGCGCAGGGAACGGCGTGCGCGGCGAAAACCATTCAGTACCACGGTGCCTGGTAGGTATAACGTTGGc                                                                                                                           <  2:7576723/140‑1 (MQ=255)

           gggTGCCGGAGATTCGTTCATTGCCGGATTCCTTTGCGGCTGGTCTGCGGGGATGACATTACCGCAGGCGATAGCGCAGGGAACGGCGTGCGCGGCGAAAACCATTCAGTACCACGGTGCCTGGTAGGTATAACGTTGGc                                                                                                                           <  2:8688241/140‑1 (MQ=255)

            ggTGCCGGAGATTCGGTCATTGCCGGATTCCTTTGCGGCTGGTCTGCGGGGATGACATTACCGCAGGCGATAGCGCAGGGAACGGCGTGCGCGGCGAAAACCATTCAGTACCACGGTGCCTGGTAGGTATAACGTTGGCg                                                                                                                          <  2:8153228/140‑1 (MQ=255)

               gCCGGAGATTCGTTCATTGCCGGATTCCTTTGCGGCTGGTCTGCGGGGATGACATTACCGCAGGCGATAGCGCAGGGAACGGCGTGCGCGGCGAAAACCATTCAGTACCACGGTGACTGGTAGGTATAACGTTGGCGTGa                                                                                                                       >  2:635685/1‑140 (MQ=255)

                ccGGAGATTCGTTCATTGCCGGATTCCTTTGCGGCTGGTCTGCGGGGATGACATTACCGCAGGCGATAGCGCAGGGAACGGCGTGCGCGGCGAAAACCATTCAGTACCACGGTGCCTGGTAGGTATAACGTTGGCGTGAg                                                                                                                      >  1:611039/1‑140 (MQ=255)

                        tCGTTCATTGCCGGATTCCTTTGCGGCTGGTCTGCGGGGATGACATTACCGCAGGCGATAGCGCAGGGAACGGCGTGCGCGGCGAAAACCATTCAGTACCACGGTGCCTGGTAGGTATAACGTTGGCGTGAGCATCTTCa                                                                                                              <  1:8175468/140‑1 (MQ=255)

                        tCGTTCATTGCCGGATTCCTTTGCGGCTGGTCTGCGGGGATGACATTACCGCAGGCGATAGCGCAGGGAACGGCGTGCGCGGCGAAAACCATTCAGTACCACGGTGCCTGGTAGGTATAACGTTGGCGTGAGCATCTTCa                                                                                                              >  1:104739/1‑140 (MQ=255)

                        tCGTTCATTGCCGGATTCCTTTGCGGCTGGTCTGCGGGGATGACATTACCGCAGGCGATAGCGCAGGGAACGGCGTGCGCGGCGAAAACCATTCAGTACCACGGTGCCTGGTAGGTATAACGTTGGCGTGAGCATCTTCa                                                                                                              <  2:4181012/140‑1 (MQ=255)

                          gTTCATTGCCGGATTCCTTTGCGGCTGGTCTGCGGGGATGACATTACCGCAGGCGATAGCGCAGGGAACGGCGTGCGCGGCGAAAACCATTCAGTACCACGGTGCCTGGTAGGTATAACGTTGGCGTGAGCATCTTCACg                                                                                                            <  2:63916/140‑1 (MQ=255)

                           ttCATTGCCGGATTCCTTTGCGGCTGGTCTGCGGGGATGACATTACCGCAGGCGATAGCGCAGGGAACGGCGTGCGCGGCGAAAACCATTCAGTACCACGGTGCCTGGTAGGTATAACGTTGGCGTGAGCATCTTCACGc                                                                                                           >  1:8425952/1‑140 (MQ=255)

                           ttCATTGCCGGATTCCTTTGCGGCTGGTCTGCGGGGATGACATTACCGCAGGCGATAGCGCAGGGAACGGCGTGCGCGGCGAAAACCATTCAGTACCACGGTGCCTGGTAGGTATAACGTTGGCGTGAGCATCTTCACGc                                                                                                           <  2:3076429/140‑1 (MQ=255)

                             cATTGCCGGATTCCTTTGCGGCTGGTCTGCGGGGATGACATTACCGCAGGCGATAGCGCAGGGAACGGCGTGCGCGGCGAAAACCATTCAGTACCACGGTGCCTGGTAGGTATAACGTTGGCGTGAGCATCTTCACGCCa                                                                                                         <  2:4768703/140‑1 (MQ=255)

                                tGCCGGATTCCTTTGCGGCTGGTCTGCGGGGATGACATTACCGCAGGCGATAGCGCAGGGAACGGCGTGCGCGGCGAAAACCATTCAGTACCACGGTGCCTGGTAGGTATAACGTTGGCGTGAGCATCTTCACGCCAACg                                                                                                      >  1:1497210/1‑140 (MQ=255)

                                   cGGATTCCTTTGCGGCTGGTCTGCGGGGATGACATTACCGCAGGCGATAGCGCAGGGAACGGCGTGCGCGGCGAAAACCATTCAGTACCACGGTGCCTGGTAGGTATAACGTTGGCGTGAGCATCTTCACGCCAACGTGc                                                                                                   <  1:207251/140‑1 (MQ=255)

                                     gATTCCTTTGCGGCTGGTCTGCGGGGATGACATTACCGCAGGCGATAGCGCAGGGAACGGCGTGCGCGGCGAAAACCATTCAGTACCACGGTGCCTGGTAGGTATAACGTTGGCGTGAGCATCTTCACGCCAACGTGCTg                                                                                                 >  1:5973397/1‑140 (MQ=255)

                                     gATTCCTTTGCGGCTGGTCTGCGGGGATGACATTACCGCAGGCGATAGCGCAGGGAACGGCGTGCGCGGCGAAAACCATTCAGTACCACGGTGCCTGGTAGGTATAACGTTGGCGTGAGCATCTTCACGCCAACGTGCTg                                                                                                 >  2:3498348/1‑140 (MQ=255)

                                             tGCGGCTGGTCTGCGGGGATGACATTACCGCAGGCGATAGCGCAGGGAACGGCGTGCGCGGCGAAAACCAGTCAGAACCACGGGGCCTGGTAGGGATAACGTTGGCGGGAGCATCTTCACGCCAACGTGCTGTTACTTGc                                                                                         >  2:5148457/1‑140 (MQ=255)

                                                  cTGGTCTGCGGGGATGACATTACCGCAGGCGATAGCGCAGGGAACGGCGTGCGCGGCGAAAACCATTCAGTACCACGGTGCCTGGTAGGTATAACGTTGGCGTGAGCATCTTCACGCCAACGTGCTGTTACTTGCCGGaa                                                                                    >  2:3166944/1‑140 (MQ=255)

                                                    ggTCTGCGGGGATGACATTACCGCAGGCGATAGCGCAGGGAACGGCGTGCGCGGCGAAAACCATTCAGTACCACGGTGCCTGGTAGGTATAACGTTGGCGTGAGCATCTTCACGCCAACGTGCTGTTACTTGCCGGaaaa                                                                                  >  2:1978187/1‑140 (MQ=255)

                                                    ggTCTGCGGGGATGACATTACCGCAGGCGATAGCGCAGGGAACGGCGTGCGCGGCGAAAACCATTCAGTACCACGGTGCCTGGTAGGTATAACGTTGGCGTGAGCATCTTCACGCCAACGTGCTGTTACTTGCCGGaaaa                                                                                  >  1:7588846/1‑140 (MQ=255)

                                                    ggTCTGCGGGGATGACATTACCGCAGGCGATAGCGCAGGGAACGGCGTGCGCGGCGAAAACCATTCAGTACCACGGTGCCTGGTAGGTATAACGTTGGCGTGAGCATCTTCACGCCAACGGGCTGTTACTTGCCGGaaaa                                                                                  >  2:6221191/1‑140 (MQ=255)

                                                      tCTGCGGGGTTGACATTACCGCAGGCGATAGCGCAGGTAAGGGCGTGCGCGGCGAAAACCTTTCTGTACCACTGTGCCTGGTAGGTATAAGTTTGGCGTGAGAATTTTCACGCCAACGTGCTGTTACTTGCCGGAAAACg                                                                                <  1:4668910/140‑1 (MQ=255)

                                                      tCTGCGGGGATGACATTACCGCAGGCGATAGCGCAGGGAACGGCGTGCGCGGCGAAAACCATTCAGTACCACGGTGCCTGGTAGGTATAACGTTGGCGTGAGCATCTTCACGCCAACGTGCTGTTACTTGCCGGAAAACg                                                                                <  2:8880397/140‑1 (MQ=255)

                                                           ggggATGACATTACCGCAGGCGATAGCGCAGGGAACGGCGTGCGCGGCGAAAACCATTCAGTACCACGGTGCCTGGTAGGTATAACGTTGGCGTGAGCATCTTCACGCCAACGTGCTGTTACTTGCCGGAAAACGACCCt                                                                           <  2:7807739/140‑1 (MQ=255)

                                                              gATGACATTACCGCAGGCGAGAGCGCAGGGAACGGCGTGCGCGGCGAAAACCATTCAGTACCACGGTGCCTGGTAGGTATAACGTTGGCGTGAGCATCTTCACGCCAACGTGCTGTTACTTGCCGGAAAACGACCCtata                                                                        <  2:7364442/140‑1 (MQ=255)

                                                              gATCACATAACCGCAGGCTATAGCGCAGGTAACGGCGTGCGCGGCGAAAACAATTCAGTACCACGGTGCCTGTTAGGTATAACGTTGGCGTGACAAACTACACGCCAACGTGCTTTTACTTGCCGTAAAACGACCCtata                                                                        <  1:8022724/140‑1 (MQ=255)

                                                                tGACATTACCGCAGGCGATAGCGCAGGGAACGGCGTGCGCGGCGAAAACCATTCAGTACCACGGTGCCTGGTAGGTATAACGTTGGCGTGAGCATCTTCACGCCAACGTGCTGTTACTTGCCGGAAAACGACCCTATAAt                                                                      <  2:5985511/140‑1 (MQ=255)

                                                                    aTTACCGCTGGCGATAGCGCTGGGAACGGCGTGCGCGGCGAAAACCATTCAGTACCACGGTGCCTGGTAGGTATAACGTTGGCGTGAGCATCTTCACGCAAACGTGCTGTTACTTGCCGGAAAACGACCCTATAATCCGa                                                                  <  2:2122345/140‑1 (MQ=255)

                                                                    aTTACCGCAGGCGATAGCGCAGGGAACGGCGTGCGCGGCGAAAACCATTCAGTACCACGGTGCCTGGTAGGTATAACGTTGGCGTGAGCATCTTCACGCCAACGTGCTGTTACTTGCCGGAAAACGACCCTATAATCCGa                                                                  >  1:5897815/1‑140 (MQ=255)

                                                                       aCCGCAGGCGATAGCGCAGGGAACGGCGTGCGCGGCGAAAACCATTCAGTACCACGGTGCCTGGTAGGTATAACGTTGGCGTGAGCATCTTCACGCCAACGTGCTGTTACTTGCCGGAAAACGACCCTATAATCCGAGTa                                                               >  1:4904970/1‑140 (MQ=255)

                                                                        ccGCAGGCGATAGCGCAGGGAACGGCGTGCGCGGCGAAAACCATTCAGTACCACGGTGCCTGGTAGGTATAACGTTGGCGTGAGCATCTTCACGCCAACGTGCTGTTACTTGCCGGAAAACGACCCTATAATCCGAGTaa                                                              >  1:3541930/1‑140 (MQ=255)

                                                                         cGCAGGCGATAGCGCAGGGAACGGCGTGCGCGGCGAAAACCATTCAGTACCACGGTGCCTGGTAGGTATAACGTTGGCGTGAGCATCTTCACGCCAACGTGCTGTTACTTGCCGGAAAACGACCCTATAATCCGAGTAat                                                             >  2:7086911/1‑140 (MQ=255)

                                                                            aGGCGATAGCGCAGGGAACGGCGTGCGCGGCGAAAACCATTCAGTACCACGGTGCCTGGTAGGTATAACGTTGGCGTGAGCATCTTCACGCCAACGTGCTGTTACTTGCCGGAAAACGACCCTATAATCCGAGTAattca                                                          <  2:4338143/140‑1 (MQ=255)

                                                                            aGGCGATAGCGCAGGGAACGGCGTGCGCGGCGAAAACCATTCAGTACCACGGTGCCTGGTAGGTATAACGTTGGCGTGAGCATCTTCACGCCAACGTGCTGTTACTTGCCGGAAAACGACCCTATAATCCGAGTAattca                                                          <  1:2839062/140‑1 (MQ=255)

                                                                            aGGCGATAGCGCAGGGAACGGCGTGCGCGGCGAAAACCATTCAGTACCACGGTGCCTGGTAGGTATAACGTTGGCGTGAGCATCTTCACGCCAACGTGCTGTTACTTGCCGGAAAACGACCCTATAATCCGAGTAattca                                                          <  1:2728376/140‑1 (MQ=255)

                                                                             ggCGATAGCGCAGGGAACGGCGTGCGCGGCGAAAACCATTCAGTACCACGGTGCCTGGTAGGTATAACGTTGGCGTGAGCATCTTCACGCCAACGTGCTGTTACTTGCCGGAAAACGACCCTATAATCCGAGTAattcat                                                         >  2:5229375/1‑140 (MQ=255)

                                                                             ggCGATAGCGCAGGGAACGGCGTGCGCGGCGAAAACCATTCAGTACCACGGTGCCTGGTAGGTATAACGTTGGCGTGAGCATCTTCACGCCAACGTGCTGTTACTTGCCGGAAAACGACCCTATAATCCGAGTAattca                                                          <  2:1430911/139‑1 (MQ=255)

                                                                             ggCGATAGCGCAGGGAACGGCGTGCGCGGCGAAAACCATTCAGTACCACGGTGCCTGGTAGGTATAACGTTGGCGTGAGCATCTTCACGCCAACGTGCTGTTACTTGCAGGAAAACGACCCTATAATCCGAGTAattcat                                                         >  2:6970412/1‑140 (MQ=255)

                                                                              gCGATAGCGCAGGGAACGGCGTGCGCGGCGAAAACCATTCAGTACCCCGGTGCCTGGTAGGTATCCCGTTGGCGTGAGCATCTTCACGCCAACGTGCTGTTCCTGGCCGGAAAACGACCCTATAATCCGAGTAattcatt                                                        <  2:2709223/140‑1 (MQ=255)

                                                                              gCGATAGCGCAGGGAACGGCGTGCGCGGCGAAAACCATTCAGTACCACGGTGCCTGGTAGGTATAACGTTGGCGTGAGCATCTTCACGCCAACGTGCTGTTACTTGCCGGAAAACGACCCTATAATCCGAGTAattcatt                                                        >  1:3958714/1‑140 (MQ=255)

                                                                              gCGATAGCGCAGGGAACGGCGTGCGCGGCGAAAACCATTCAGTACCACGGTGCCTGGTAGGGATAACGTTGGCGTGAGCATCTTCACGCCAACGTGCTGTTACGTGCCGGAAAACGACCCTATAATCCGAGTAattcatt                                                        >  2:4236690/1‑140 (MQ=255)

                                                                                 atcgcgcTGGTAACGGCGGGCGCGGCGAAAACAATTCAGTACCACGGGGCGTGGTTGGTATACCGTTGGCGTGCGCATCTTCACGCCAACGTGCTGTTACTTGCCGGAAAACGACCCTATAATCCGAGTAATTCATTCtt                                                     <  1:1290374/137‑1 (MQ=255)

                                                                                      gcAGGGAACGGCGTGCGCGGCGAAAACCATTCAGTACCACGGTGCCTGGTAGGTATAACGTTGGCGTGAGCATATTCACGCCAACGTGCTGTTACTTGCCGGAAAACGACACTATAATCCGAGTAATTCATTCTTTAttt                                                <  2:2424645/140‑1 (MQ=255)

                                                                                        aGGGAACGGCGTGCGCGGCGAAAACCATTCAGTACCACGGTGCCTGGTAGGTATAACGTTGGCGTGAGCATCTTCACGCCAACGTGCTGTTACTTGCCGGAAAACGACCCTATAATCCGAGTAATTCATTCTTTATTTCa                                              >  1:3066920/1‑140 (MQ=255)

                                                                                          ggAACGGCGTGCGCGGCGAAAACCATTCAGTACCACGGTGCCTGGTAGGTATAACGTTGGCGTGAGCATCTTCACGCCAACGTGCTGTTACTTGCCGGAAAACGACCCTATAATCCGAGTAATTCATTCTTTATTTCAgg                                            >  1:1838572/1‑140 (MQ=255)

                                                                                          ggAACGGCGTGCGCGGCGAAAACCATTCAGTACCACGGTGCCTGGTAGGTATAACGTTGGCGTGAGCATCTTCACGCCAACGTGCTGTTACTTGCCGGAAAACGACCCTATAATCCGAGTAATTCATTCTTTATTTCAgg                                            >  2:5856947/1‑140 (MQ=255)

                                                                                          ggAACGGCGTGCGCGGCGAAAACCATTCAGTACCACGGTGCCTGGTAGGTATAACGTTGGCGTGAGCATCTTCACGCCAACGTGCTGTTACTTGCCGGAAAACGACCCTATAATCCGAGTAATTCATTCTTTATTTCAgg                                            >  2:3255938/1‑140 (MQ=255)

                                                                                          ggAACGGCGTGCGCGGCGAAAACCATTCAGTACCACGGTGCCTGGTAGGTATAACGTTGGCGTGAGCATCTTCACGCCAACGTGCTGTTACTTGCCGGAAAACGACCCTATAATCCGAGTAATTCATTCTTTATTTCAgg                                            >  2:2909757/1‑140 (MQ=255)

                                                                                           gAACGGCGTGCGCGGCGAAAACCATTCAGTACCACGGTGCCTGGTAGGTATAACGTTGGCGTGAGCATCTTCACGCCAACGTGCTGTTACTTGCCGGAAAACGACCCTATAATCCGAGTAATTCATTCTTTATTTCAggg                                           <  2:5663233/140‑1 (MQ=255)

                                                                                           gAACGGCGTGCGCGGCGAAAACCATTCAGTACCACGGTGCCTGGTAGGTATAACGTTGGCGTGAGCATCTACACGCCAACGTGCTGTTACTTGCCGGAAAACGACCCTATAATCCGAGTAATTCATTCTTTATTTCAggg                                           <  1:1978187/140‑1 (MQ=255)

                                                                                            aaCGGCGTGCGCGGCGAAAACCATTCAGTACCACGGTGCCTGGTAGGTATAACGTTGGCGTGAGCATCTTCACGCCAACGTGCTGTTACTTGCCGGAAAACGACCCTATAATCCGAGTAATTCATTCTTTATTTCAGGGt                                          >  1:3330800/1‑140 (MQ=255)

                                                                                              cttcGTTCGCCGAGACAACCATTCAGAACCTCGGTGACTGTTAGGTATAACGTGGGCGTGAGCATCTTCACGCCAACGTGCTGTTACTTGCCGTTAAACGACCCTATAATACGAGTAATTCATTCTTTATTTCAGGGTCg                                        <  2:6576109/137‑1 (MQ=255)

                                                                                              cGGCGTGCGCGGCGAAAACCATTCAGTACCCCGGGGCCTGGTAGGTATAACGTTGGCGTGAGCATCTTCACGCCAACGTGCTGTTACTGGCCGGAAAACGACCCTATAATCCGAGTAATTCATTCTTTATTTCAGGGTCg                                        <  1:6326218/140‑1 (MQ=255)

                                                                                              cGGCGTGCGCGGCGAAAACCATTCAGTACCACGGTGCCTGGTAGGTATAACGTTGGCGTGAGCATCTTCACGCCAACGTGCTGTTACTTGCCGGAAAACGACCCTATAATCCGAGTAATTCATTCTTTATTTCAGGGTCg                                        <  2:6067622/140‑1 (MQ=255)

                                                                                               ggCGTGCGCGGCGAAAACCATTCAGTACCACGGTGCCTGGTAGGTATAACGTTGGCGTGAGCATCTTCACGCCAACGTGCTGTTACTTGCCGGAAAACGACCCTATAATCCGAGTAATTCATTCTTTATTTCAGGGTCGa                                       <  1:7796701/140‑1 (MQ=255)

                                                                                               ggCGTGCGCGGCGAAAACCATTCAGTACCACGGTGCCTGGTAGGTATAACGTTGGCGTGAGCATCTTCACGCCAACGTGCTGTTACTTGCCGGAAAACGACCCTATAATCCGAGTAATTCATTCTTTATTTCAGGGTCGa                                       >  1:7901835/1‑140 (MQ=255)

                                                                                                 cGTGCGCGGCGAAAACCATTCAGTACCACGGTGCCTGGTAGGTATACCGTGGGCGGGAGCATCGTCACGCCAACGTGCTGTTACTTGCCGGAAAACGACCCTATACTCCGAGTAATTCATTCTTTATTTCAGGGTCGAtt                                     <  2:5311430/140‑1 (MQ=255)

                                                                                                   tGCGCGGCGAAACCCATTCATTACCACGGGGCCTGGTCGTTATACCGTTGGCGTGAGCATCTTCACGCCAACTTGCTGTTCCGTGCCGTAACACGACCCTATAATCCGAGTAATTCATTCTTTATTTCAGGGTCGATTAt                                   <  1:5342821/140‑1 (MQ=255)

                                                                                                    gcgcGGCGAAAACCATTCAGTACCACGGTGCCTGGTAGGTATAACGGTGGCGTGAGCATCTTCACGCCAACGTGCTGTTACTTGCCGGAAAACGACCCTATAATCCGAGTAATGTATTCTTTATTTCAGGGTCGATTATg                                  >  2:7770800/1‑140 (MQ=255)

                                                                                                     cgcgGCGAAAACCATTCAGTACCACGGTGCCTGGTAGGTATAACGTTGGCGTGAGCATCTTCACGCCAACGTGCTGTTACTTGCCGGAAAACGACCCTATAATCCGAGTAATTCATTCTTTATTTCAGGGTCGATTATGt                                 >  2:2333252/1‑140 (MQ=255)

                                                                                                     cgcgGCGAAAACCATTCAGTACCACGGTGCCTGGTAGGTATAACGTTGGCGTGAGCATCTTCACGCCAACGTGCTGTTACTTGCCGGAAAACGACCCTATAATCCGAGTAATTCATTCTTTATTTCAGGGTCGATTATGt                                 <  1:6493374/140‑1 (MQ=255)

                                                                                                     cgcgGCGAAAACCATTCAGTACCACGGTGCCTGGTAGGTATAACGTTGGCGTGAGCATCTTCACGCCAACGTGCTGTTACTTGCCGGAAAACGACCCTATAATCCGAGTAATTCATTCTTTATTTCAGGGTCGATTATGt                                 <  2:6862434/140‑1 (MQ=255)

                                                                                                     cgcgGCGAAAACCATTCAGTACCACGGTGCCTGGTAGGTATAACGTTGGCGTGAGCATCTTCACGCCAACGTGCTGTTACTTGCCGGAAAACGACCCTATAATCCGAGTAATTCATTCTTTATTTCAGGGTCGATTATGt                                 <  1:7335973/140‑1 (MQ=255)

                                                                                                     cgcgGCGAAAACCATGCAGTACCCCGGGGCCTGGTAGGTATAACGTTGGCGTGAGCATCTTCACGCCAACGTGCTGTTACTTGCCGGAAAACGACCCTATAATCCGAGTAATTCATTCTTTATTTCAGGGTCGATTATGt                                 <  1:5592696/140‑1 (MQ=255)

                                                                                                             acaCCATTCAGTCCCCCGGTGCCTGGTCGGTATAACGTTGGCGTGAGCATCTTCACGCCACCGTGCTGTTACTTGCCGGACAACGACCCTATCATCCGAGTAATTCATTCTTTATTTCCGGGTCGATTATGTCAGCTACg                         <  1:3621153/138‑1 (MQ=255)

                                                                                                             aaaCCATTCAGTACCACGGTGCCTGGTAGGTATAACGTTGGCGTGAGCATCTTCACGCCAACGTGCTGTTACTTGCCGGAAAACGACCCTATAATCCGAGTAATTCATTCTTTATTTCAGGGTCGATTATGTCAGCTACg                         <  1:6737732/140‑1 (MQ=255)

                                                                                                             aaaCCATTCAGTACCACGGTGCCTGGTAGGTATAACGTTGGCGTGAGCATCTTCACGCCAACGTGCTGTTACTTGCCGGAAAACGACCCTATAATCCGAGTAATTCATTCTTTATTTCAGGGTCGATTATGTCAGCTACg                         <  1:579076/140‑1 (MQ=255)

                                                                                                             aaaCCATTCAGTACCACGGTGCCTGGTAGGTATAACGTTGGCGTGAGCATCTTCACGCCAACGTGCTGTTACTTGCCGGAAAACGACCCTATAATCCGAGTAATTCATTCTTTATTTCAGGGTCGATTATGTCAGCTACg                         <  1:8623720/140‑1 (MQ=255)

                                                                                                             aaaCCATTCAGTACCACGGTGCCTGGTAGGTATAACGTTGGCGTGAGCATCTTCACGCCAACGTGCTGTTACTTGCCGGAAAACGACCCTATAATCCGAGTAATTCATTCTTTATTTCAGGGTCGATTATGTCAGCTACg                         <  1:5172370/140‑1 (MQ=255)

                                                                                                             aaaCCATTCAGTACCACGGTGCCTGGTAGGTATAACGTTGGCGTGAGCATCTTCACGCCAACGTGCTGTTAATTGCCGGAACACGACCCTATAATCCGAGTAATTCATTCTTTATTTCAGGGTCGATTATGTCAGCTACg                         <  1:2375905/140‑1 (MQ=255)

                                                                                                             aaaCCATTCAGTACCACGGTGCCTGGTAGGTATAACGGTGGCGTGAGCATCTTCACGCCAACGTGCTGTTACTTGCCGGAAAACGACCCTATAATCCGAGTAATTCATTCTTTATTTCAGGGTCGATTATGTCAGCTACg                         <  2:9274868/140‑1 (MQ=255)

                                                                                                               aCCATTCAGTACCACGGTGCCTGGTAGGTATAACGTTGGCGTGAGCATCTTCACGCCAACGTGCTGTTACTTGCCGTAAAACGACCCTATAATCCGAGTAATTCATTCTTTATTTCAGGGTCGATTATGTCAGCTACGGa                       <  2:4513305/140‑1 (MQ=255)

                                                                                                                  aTTCAGTACCACGGTGCCTGGTAGGTATAACGTTGGCGTGAGCATCTTCACGCCAACGTGCTGTTACTTGCCGGAAAACGACCCTATAATCCGAGTAATTCATTCTTTATTTCAGGGTCGATTATGTCAGCTACGGACCg                    <  2:5523564/140‑1 (MQ=255)

                                                                                                                  aTTCAGTACCACGGTGCCTGGGAGGTATAACGTGGGCGGCCGCATCTTCACGCCAACGGGCTGTTACTTGCCGGAAAAAGACCCTATACGCCGAGTAATTCATTCTTTATTTCACGTTCGATTAGGTCAGCTACGGACCg                    <  2:1650987/140‑1 (MQ=255)

                                                                                                                     cAGTACCACGGTGCCTGGTAGGTATAACGTTGGCGTGAGCATCTTCACGCCAACGTGCTGTTACTTGCCGGAAAACGACCCTATAATCCGAGTAATTCATTCTTTATTTCAGGGTCGATTATGTCAGCTACGGACCGCTa                 <  1:4799204/140‑1 (MQ=255)

                                                                                                                     cAGTACCACGGTGCCTGGTAGGTATAACGTTGGCGTGAGCATCTTCACGCCAACGTGCTGTTACTTGCCGGAAAACGACCCTATAATCCGAGTAATTCATTCTTTATTTCAGGGTCGATTATGTCAGCTACGGACCGCTa                 <  1:6876835/140‑1 (MQ=255)

                                                                                                                     cAGTACCACGGTGCCTGGTAGGTATAACGTTGGCGTGAGCATCTTCACGCCAACGTGCTGTTACTTGCCGGAAAACGACCCTATAATCCGAGTAATTCATTCTTTATTTCAGGGTCGATTATGTCAGCTACGGACCGCTa                 <  2:3872957/140‑1 (MQ=255)

                                                                                                                       gTACCACGGTGCCTGGTAGGTATAACGTTGGCGTGAGCATCTTCACGCCAACGTGCTGTTACTTGCCGGAAAACGACCCTATAATCCGAGTAATTCATTCTTTATTTCAGGGTCGATTATGTCAGCTACGGACCGCTAct               <  2:492929/140‑1 (MQ=255)

                                                                                                                        tACCACGGTGCCTGGTAGGTATAACGTTGGCGTGAGCATCTTCACGCCAACGTGCTGTTACTTGCCGGAAAACGACCCTATAATCCGAGTAATTCATTCTTTATTTCAGGGTCGATTATGTCAGCTACGGACCGCTActc              <  1:8943271/140‑1 (MQ=255)

                                                                                                                            aCGGTGCCTGGTAGGTATAACGTTGGCGTGAGCATCTTCACGCCAACGTGCTGTTACTTGCCGGAAAACGACCCTATAATCCGATTAATTCATTCTTTATTTCAGGGTCGATTATGTCAGCTACGGACCGCTACTCtcat          <  2:7506034/140‑1 (MQ=255)

                                                                                                                             cGGTGCCTGGTAGGTATAACGTTGGCGTGAGCATCTTCACGcc                                                                                                          <  1:5084099/43‑1 (MQ=255)

                                                                                                                               gTGCCTGGTAGGTATAACGTTGGCGTGAGCATCTTCACGCCAACGTGCTGTTACTTGCCGGAAAACGACCCTATAATCCGAGTAATTCATTCTTTATTTCAGGGTCGATTATGTCAGCTACGGACCGCTACTCTCATCaa       <  2:25531/140‑1 (MQ=255)

                                                                                                                                tGCCTGGTAGGTATAACGTTGGCGTGAGCATCTTCACGCCAACGTGCTGTTACTTGCCGGAAAACGACCCTATAATCCGAGTAATTCATTCTTTATTTCAGGGTCGATTATGTCAGCTACGGACCGCTACTCTCATCAAc      <  1:298678/140‑1 (MQ=255)

                                                                                                                                tGCCTGGTAGGTATAACGTTGGCGTGAGCATCTTCACGCCAACGTGCTGTTACTTGCCGGAAAACGACCCTATAATCCGAGTAATTCATTCTTTATTTCAGGGTCGATTATGTCAGCTACGGACCGCTACTCTCATCAAc      >  1:4499825/1‑140 (MQ=255)

                                                                                                                                tGCCTGGTAGGTATAACGTTGGCGTGAGCATCTTCACGCCAACGTGCTGTTACTTGCCGGAAAACGACCCTATAATCCGAGTAATTCATTCTTTATTTCAGGGTCGATTATGTCAGCTACGGACCGCTACTCTCATCAAc      >  2:1478121/1‑140 (MQ=255)

                                                                                                                                tGCCTGGTAGGTATAACGTTGGCGTGAGCATCTTCACGCCAACGTGCTGTTACTTGCCGGAAAACGACCCTATAATCCGAGTAATTCATTCTTTATTTCAGGGTCGATTATGTCAGCTACGGACCGCTACTCTCATCAAc      >  2:1301586/1‑140 (MQ=255)

                                                                                                                                tGCCTGGAAGGTATAACGTTCGCGTGAGCATCTTCACGCCACCGTGCTGTTACTTGCCGGAAAACGACCCTATAATCCGAGTAATTCATTCATTATTTCAGGGTCGATTATGTCAGCTACGGACCGCTACTCTCATCAAc      <  2:1762954/140‑1 (MQ=255)

                                                                                                                                  ccTGGTAGGTATAACGTTGGCGTGAGCATCTTCACGCCAACGTGCTGTTACTTGCCGGAAAACGACCCTATAATCCGAGTAATTCATTCTTTATTTCAGGGTCGATTATGTCAGCTACGGACCGCTACTCTCATCAActc    >  2:370685/1‑140 (MQ=255)

                                                                                                                                  ccTGGTAGGTATAACGTTGGCGTGAGCATCTTCACGCCAACGTGCTGTTACTTGCCGGAAAACGACCCTATAATCCGAGTAATTCATTCTTTATTTCAGGGTCGATTATGTCAGCTACGGACCGCTACTCTCATCAActc    >  1:706224/1‑140 (MQ=255)

                                                                                                                                  ccTGGTAGGTATAACGTTGGCGTGAGCATCTTCACGCCAACGTGCTGTTACTTGCCGGAAAACGACCCTATAATCCGAGTAATTCATTCTTTATTTCAGGGTCGATTATGTCAGCTACGGACCGCTACTCTCATCAActc    >  2:7207726/1‑140 (MQ=255)

                                                                                                                                  ccTGGTAGGTATAACGTTGGCGTGAGCATCTTCACGCCAACGTGCTGTTACTTGCCGGAAAACGACCCTATAATCCGAGTAATTCATTCTTTATTTCAGGGTCGATTATGTCAGCTACGGACCGCTACTCTCATCAActc    >  1:1471355/1‑140 (MQ=255)

                                                                                                                                    tGGTAGGTATAACGTTGGCGTGAGAATCTTCACGCCAACGTGCTGTTACTTGCCGGAACACGACCCTATAATCCGAGTAATTCATTCTTTATTTCAGGGTCGATTATGTCAGCTACGGACCGCTACTCTCATCAActcct  <  1:4276117/140‑1 (MQ=255)

ATCGACACCATGGGTGCCGGAGATTCGTTCATTGCCGGATTCCTTTGCGGCTGGTCTGCGGGGATGACATTACCGCAGGCGATAGCGCAGGGAACGGCGTGCGCGGCGAAAACCATTCAGTACCACGGTGCCTGGTAAGTATAACGTTGGCGTGAGCATCTTCACGCCAACGTGCTGTTACTTGCCGGAAAACGACCCTATAATCCGAGTAATTCATTCTTTATTTCAGGGTCGATTATGTCAGCTACGGACCGCTACTCTCATCAACTCCT  >  NZ_CP025268/3504501‑3504772

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`